ATBECHDEDKESFRGBGRITLILUNLSEMCPTIESILTLVFIROMKCYALTRBGCZEEHUPLSKBAHRIS..MTNORSMESM..................*J2.0.21 - 2999001/03707143CORRECTED EUROPEAN PATENT SPECIFICATIONB9B120230412W1deAnsprüche ENenClaims ENfrRevendications ENEP18795687.5201811062020052120211115enenen1738274120171106EP2023041220231520200916202038202212282022522022071420230412202315C07D 495/04 20060101AFI20190510BHEP A61P 35/00 20060101ALI20190510BHEP A61P 5/00 20060101ALI20190510BHEP A61K 31/4422 20060101ALI20190510BHEP C07D 495/04 20130101 FI20180328BHEP A61P 35/00 20180101 LI20180328BHEP A61P 5/00 20180101 LI20180328BHEP deNEUE ANALOGA ALS ANDROGEN-REZEPTOR UND GLUCOCORTICOID-REZEPTOR-MODULATORENenNEW ANALOGS AS ANDROGEN RECEPTOR AND GLUCOCORTICOID RECEPTOR MODULATORSfrNOUVEAUX ANALOGUES EN TANT QUE MODULATEURS DES RÉCEPTEURS D'ANDROGÈNE ET DES RÉCEPTEURS DES GLUCOCORTICOÏDESWO-A2-2017/059401US-A- 4 659 717KURZ, GuidoLluís Domenech i Montaner 6, Escalera 5, Piso 3AE-08950 Esplugues Llobregat, BarcelonaESCAMACHO GÓMEZ, JuanEstafeta 61 5-EE-31001 Pamplona, NavarraESOncostellae, S.L.101706129P15232EPPCEdificio FEUGA, D5 Campus Vida. Rúa Lope Gómez de Marzoa s/n15705 Santiago de CompostelaESABG Intellectual Property Law, S.L.100061541Avenida de Burgos, 16D Edificio Euromor28036 MadridESALATBEBGCHCYCZDEDKEEESFIFRGBGRHRHUIEISITLILTLULVMCMKMTNLNOPLPTRORSSESISKSMTRBA20200521ME20200521EP201808036720181106enWO201908672020190509201919 Field of the invention

This invention relates to novel pharmaceutically-useful, optionally substituted dihydropyridines derivatives which are useful as modulators of nuclear receptors, such as receptors selected from androgen receptor and glucocorticoid receptor. The compounds are of potential utility in the treatment of diseases and conditions mediated by the nuclear receptors selected from androgen receptor and glucocorticoid receptor, such as cancer. The invention also relates to the use of such compounds as medicaments, to pharmaceutical compositions containing them, and to synthetic routes for their production.

Background of the invention

Nuclear receptors constitute a large superfamily of ligand-dependent transcription factors that are involved in many physiologic and developmental processes and are linked to a wide range of human diseases. That makes them attractive targets for drug discovery. In fact, approximately 13% of all FDA-approved drugs act on nuclear receptors. (Mangelsdorf DJ. et al, The nuclear receptor superfamily: the second decade, Cell 1995, 83, 835-839).

Nuclear receptors bind specific DNA elements in the regulatory regions of genes via a highly-conserved DNA-binding domain (DBD) and specific ligands via another highly conserved domain, the ligand binding domain (LBD), which consists of a series of approximately 12 helices that form a hydrophobic pocket. The binding of ligands in the pocket induces conformational changes in the receptor that affect the recruitment of coregulatory molecules (cofactors) that stimulate (co-activators) or repress (corepressors) transcription. (Huang P. et al, Structural overview of the nuclear receptor superfamily: Insights into physiology and therapeutics, Annu Rev Physiol 2010, 72, 247-272).

The androgen receptor (AR) belongs to the subfamily of steroid hormone-activated nuclear receptors which include also the estrogen, glucocorticoid, minerocorticoid and progesterone receptors (ER, GR, MR and PR). In the cytoplasm, unliganded AR is stabilized by heat shock proteins. Upon ligand binding of testosterone or dihydrotestosterone (DHT), a conformational change arises in the AR causing dissociation of specific chaperones, dimerization and phosphorylation of AR, and AR translocation to the nucleus. The DNA-binding domain (DBD) in AR binds to androgen response elements (AREs) inside the nucleus, causing recruitment of DNA transcriptional machinery and gene transcription. AR is found in a variety of tissues throughout the human body including prostate, scalp, skin and muscle.

Androgen receptor is the primary therapeutic target in prostate cancer which is a major health problem in industrialized countries, as it represents the second main cause of death from cancer in men. In newly diagnosed patients, the tumor is frequently confined to the prostate where it can be removed surgically or treated by radiotherapy. When metastases are detected or rising prostate-specific antigen (PSA) biomarker levels indicate disease progression, the first course of treatment is lowering the supply of androgens to the tumor (androgen deprivation therapy, ADT) because, in the primary stage, prostate tumor growth is androgen-dependent. Androgen levels can be decreased by surgical castration (elimination of testosterone production in the testis) or by medical treatment with antiandrogens (deactivation of AR), LHRH (luteinizing hormone-releasing hormone) agonists or antagonists (suppression of testosterone production in the testis) and CYP17 or 5alpha-reductase inhibitors (inhibition of androgen biosynthesis).

Initially ADT is effective in controlling the disease, but within a few years tumor cells usually evolve mechanisms for continued growth under conditions of androgen depletion and the cancer becomes what is known as recurrent or castration-resistant prostate cancer (CRPC). Most of the mechanisms promoting CRPC are still AR-dependent and include intratumoral androgen synthesis, increased expression of AR through gene amplification or overexpression, upregulation of coactivator proteins that augment AR activity, acquisition of mutations within the AR protein that increase its activity in response to antagonists or other steroid hormones, constitutively active AR splice variants, and signaling through alternative pathways (e.g. induced by glucocorticoid receptor overexpression). (Watson PA. et al, Emerging mechanisms of resistance to AR inhibitors in prostate cancer, Nat Rev Cancer 2015, 15, 701-711).

There is no cure for CRPC and current medication provides only a modest overall survival benefit of 4 to 5 months. The median survival of patients with advanced metastatic prostate cancer, who have failed androgen deprivation therapy, was typically 16 to 20 months in 2009. (Thoreson GR. et al, Emerging therapies in castration resistant prostate cancer, Can J Urol 2014, 21, 98-105).

Nonsteroidal antiandrogens have been preferred over steroidal compounds for prostate cancer because they are more selective and have fewer side effects. (Elancheran R, Recent discoveries and developments of androgen receptor based therapy for prostate cancer, Med Chem Commun 2015, 6, 746-768).

Therefore, there is still a great need for new treatment options including improved, non-steroidal AR antagonists that could be useful for the treatment of both primary and especially castrate-resistant prostate cancer.

AR antagonists may also be useful for the treatment of other conditions, disorders and diseases which are regulated by the androgen receptor including but not limited to breast cancer (Hickey TE et al, Expression of androgen receptor splice variants in clinical breast cancer, Oncotarget 2015, advanced publication Nov 5), epithelial ovarian cancer, benign prostate hyperplasia, alopecia and acne (Jie JL, et al, Rational design and synthesis of 4-((1R,2R)-2-hydroxycyclohexyl)-2(trifluoromethyl)benzonitrile (PF-998425), a novel, nonsteroidal androgen receptor antagonist devoid of phototoxicity for dermatological indications, J Med Chem 2008, 51, 7010-7014), hirsutism and polycystic ovary syndrome.

As has been pointed out previously, glucocorticoid receptor might play a role in one of the mechanisms promoting CRPC. At first, the hypothesis that GR can confer resistance may seem inconsistent with clinical evidence that glucocorticoid administration can be beneficial to some patients with CRPC. This apparent contradiction is explained by the fact that glucocorticoids inhibit adrenocorticotropic hormone (ACTH) production by the pituitary gland, which results in reduced androgen levels. This androgen-lowering activity explains the decline in serum PSA levels that is observed in men taking prednisone alone, which was documented in the comparator arm of the Phase III clinical trial that led to approval of abiraterone for chemotherapy-naive CRPC. However, in men with prostate cancers that express high levels of GR, this androgen-lowering benefit would be counteracted by GR activation in tumour cells. In this setting, a more effective treatment strategy could be combined inhibition of AR and GR, as is currently being explored in an early-phase clinical trial of enzalutamide in combination with the GR antagonist mifepristone/RU486 (ClinicalTrials.gov identifier: NCT02012296). One potential confounder of this study is the fact that mifepristone also has a high binding affinity for AR and can function as an AR agonist. Therefore, mifepristone treatment could unintentionally result in AR activation through agonism by displacing the potent antagonism of enzalutamide. (Watson PA. et al, Emerging mechanisms of resistance to androgen receptor inhibitors in prostate cancer, Nature Reviews Cancer | AOP, published online 13 November 2015, and references therein).

There are studies showing that GR agonists, such as dexamethasone, are sufficient to induce enzalutamide resistance, whereas a GR antagonist could restore sensitivity. Currently, with the widespread use of enzalutamide, resistance is increasing as a clinical problem, in which activation of the glucocorticoid signalling pathway seems to have an important role. These findings suggest the role that glucocorticoids might have in promoting tumour growth. A major implication of these studies is that glucocorticoids might promote tumour growth and progression in men whose cancers express the GR. Thus, new strategies that block both AR signalling and GR signalling might be necessary and efforts to develop GR-specific antagonists that do not have off-target effects on the AR would be useful to prevent or overcome enzalutamide resistance. As opposed to AR inhibition, which can cause unpleasant but tolerable adverse effects, total GR inhibition can be lethal. (Narayanan S, et al, Androgen-glucocorticoid interactions in the era of novel prostate cancer therapy, NATURE REVIEWS | UROLOGY, doi:10.1038/nrurol.2015.254, Published online 8 Dec 2015, and references therein).

Glucocorticoids (GR agonists) are strong immunosuppressive and antiinflammatory agents that are widely used as co-medication in cancer therapy of solid tumors to alleviate adverse effects, reduce toxicity and protect normal tissue. However, glucocorticoids may interfere with therapeutic efficacy of anti-cancer medication, e.g. by inhibiting apoptosis and promoting proliferation. Therefore, there have been suggestions to minimize the use of glucocorticoids in anti-cancer therapy. Mechanistically, dexamethasone (an important glucocorticoid drug) has been found to suppress immune response by enhancing expression of the T cell checkpoint proteins PD-1 and CTLA-4. GR antagonist mifepristone has been shown to reverse this effect (K. Xing et al., Dexamethasone enhances programmed cell death 1 (PD-1) expression during T cell activation: an insight into the optimum application of glucocorticoids in anti-cancer therapy, BMC Immunology 2015, 16, 39; M. Xia et al., Dexamethasone enhances CTLA-4 expression during T cell activation, Cell Mol Life Sci 1999, 55, 1649-1659) .

GR antagonists that have the opposite effect to glucocorticoids might therefore be useful as activators of the immune system for the treatment of solid tumors as a single agent and in combination with checkpoint inhibitors. Currently, Mifepristone is being studied in cancer patients, wherein stress-response mechanisms including the hypothalamic-pituitary-adrenal (HPA) axis are frequently activated and dysregulated.

High levels and altered diurnal rhythms of cortisol, a glucocorticoid hormone involved in stress signaling, inflammation and metabolism, have been correlated with poor prognosis of terminal cancer patients, e.g. lung, breast, colorectal and ovarian cancer (H.M. Kimet al., Random serum cortisol as a predictor for survival of terminally ill patients with cancer, Am J Hosp Pall Med 2016, 33, 281-285; A. Schrepf et al., Diurnal cortisol and survival in epithelial ovarian cancer, Psychoneuroendocrinology 2015, 53, 256-267).

Recently it was shown that cortisol production is a common feature of a broad spectrum of malignant cells and that cancer-derived cortisol inhibits tumor-specific CD8+ T cell proliferation in a GR-specific fashion. The effect was inhibited in the presence of GR antagonist mifepristone/RU486. These data suggest that the production of cortisol by tumor cells may have an important immunoregulatory function and that GR antagonists may have a therapeutic potential as reactivators of the immune system (N. Cirillo et al., Characterisation of the cancer-associated glucocorticoid system: key role of 11b-hydroxysteroid dehydrogenase type 2, Br. J. Cancer 2017, advanced online publication 1-10, doi: 10.1038/bjc.2017.243).

Currently, there are few glucocorticoid receptor antagonists marketed and in clinical assays. Among them is mifepristone, a synthetic steroid compound which has been investigated as an antiglucocorticoid drug in the treatment of persistent or recurrent Cushing's disease. Its affinity to the progesterone receptor is a main drawback. (Schteingart D.E., Drugs in the medical treatment of Cushing's syndrome, Expert Opin. Emerging Drugs (2009) 14(4):661-671). Other glucocorticoid receptor antagonists are in (pre)clinical development (Kach J. et al, Selective glucocorticoid receptor modulators (SGRMs) delay castrate-resistant prostate cancer growth, mct.aacrjournals.org on April 21, 2017; Hunt H.J. et al, The Identification of the Clinical Candidate (R)-(1-(4-fluorophenyl)-6-((1-methyl-1Hpyrazol-4-yl)sulfonyl)-4,4a, 5, 6,7,8-hexahydro-1H-pyrazolo[3,4-g]isoquinolin-4a-yl) (4-(trifluoromethyl)pyridin-2-yl)methanone (CORT125134): A Selective Glucocorticoid Receptor (GR) Antagonist, J. Med. Chem, DOI: 10.1021/acs.jmedchem.7b00162 • Publication Date (Web): 03 Apr 2017).

The listing or discussion of an apparently prior-published document in this specification should not necessarily be taken as an acknowledgement that the document is part of the state of the art or is common general knowledge.

1,4-dihydropyridines are an important class of cardiovascular drugs such as nifedipine, nitrendipine, amlodipine and many other analogs which exert their antihypertensive and antianginal actions by blocking L-type calcium channels. The 1,4-dihydropyridine nucleus is also a privileged scaffold that can, when appropriately substituted, interact at diverse receptors and ion channels, providing a wide range of biological activities (P. loan et al., 1,4-Dihydropyridine scaffold in medicinal chemistry, The story so far and perspectives. (Part 1): Action in Ion Channels and GPCRs. Curr. Med. Chem. 2011, 18, 4901-4922; E. Carosati et al., 1,4-Dihydropyridine Scaffold in Medicinal Chemistry, The story so far and perspectives (Part 2): Action in Other Targets and Antitargets, Curr. Med. Chem. 2012, 19, 4306-4323).

International patent application WO 2005/016885 A2 discloses various dihydropyridines with inhibitory activity against beta-adrenergic receptors and L-type calcium channels that may be useful for the treatment of heart disease. However, there is no mention that these compounds may be useful for the treatment of cancer.

European patent application EP 0 217 530 A1 discloses dihydropyridines derivatives as vasodilators useful in the treatment of stroke, angina and migraine. In addition, the compounds are useful as antiasthma agents. Among the disclosed compounds there are 4-benzothiophene-1,4-dihydropyridine-3,5-diester derivatives which are not active as modulators of androgen receptor, therefore are not included in the scope of the present invention.

Patent application US 2016/0151388 A1 dislcoses a method and compositions related to glucocorticoid receptor antagonist in prostate cancer, alone or in combination with an androgen receptor antagonist. The prostate cancer may be one that has become resitant to androgen deprivation therapy, for example, by increase in glucocorticoid receptor expression and /or activity.

Patent application WO 2017 059401 A2 discloses derivatives of dihydropyridine with an annellated ring as ligands on the androgen receptor whereby the dihydropyridine ring is fused to an aromatic ring and a non-aromatic ring. The disclosed compounds are useful for treating hormone refractory prostate cancer.

The problem to be solved by the present invention is to provide compounds as modulators of nuclear receptors selected from androgen receptor and glucocorticoid receptor.

SUMMARY OF THE INVENTION

In one of its aspects (aspect 1), the present invention refers to dihydropyridine derivative of formula (I): wherein:

each R6 and R7 independently represents: and pharmaceutically acceptable salts thereof.

Other aspects of the present invention are:
In a second aspect, the present invention refers to processes for the preparation of the compounds defined in the first aspect.

In a third aspect, the present invention refers to pharmaceutical compositions comprising an effective amount of a compound defined in the first aspect.

In a fourth, the present invention refers to a combination product comprising a compound as defined in the first aspect and another therapeutic agent selected from agents for treating prostate cancer, castration-resistant prostate cancer, pancreatic cancer, bladder cancer, renal cancer, gastric cancer, lung cancer, breast cancer, colon cancer, colorectal cancer, ovarian cancer, and other solid tumours, melanoma, metastasizing cancers, benign prostate hyperplasia, polycystic ovary syndrome (PCOS), hair loss, hirsutism, acne, hypogonadism, muscle wasting diseases, cachexia and Cushing's syndrome, anti-psychotic drug induced weight gain, obesity, post-traumatic stress disorder and alcoholism.

In a fifth aspect, the present invention relates to a compound as defined in the first aspect for use as a medicament.

In a sixth aspect the present invention relates to a compound as defined in the first aspect for use in the treatment of a disease or pathological condition selected from the group consisting of cancer, prostate cancer, castration-resistant prostate cancer, pancreatic cancer, bladder cancer, renal cancer, gastric cancer, lung cancer, breast cancer, colon cancer, colorectal cancer, ovarian cancer, and other solid tumours, melanoma, metastasizing cancers, benign prostate hyperplasia, polycystic ovary syndrome (PCOS), hair loss, hirsutism, acne, hypogonadism, muscle wasting diseases and cachexia, and Cushing's syndrome, anti-psychotic drug induced weight gain, obesity, post-traumatic stress disorder and alcoholism.

In a seventh aspect, the present invention relates to a pharmaceutical composition comprising a compound as defined the first aspect of the invention and a pharmaceutically acceptable diluent or carrier.

As it is said before, the invention relates to dihydropyridine derivatives for use in the treatment or prevention of diseases known to be susceptible to amelioration by treating with modulators of nuclear receptors, in particular by modulators of nuclear receptors selected from androgen receptor and glucocorticoid receptor. Such diseases are, for example cancer, prostate cancer, castration-resistant prostate cancer, pancreatic cancer, bladder cancer, renal cancer, gastric cancer, lung cancer, breast cancer, colon cancer, colorectal cancer, ovarian cancer, and other solid tumours, melanoma, metastasizing cancers, benign prostate hyperplasia, polycystic ovary syndrome (PCOS), hair loss, hirsutism, acne, hypogonadism, muscle wasting diseases and cachexia, and Cushing's syndrome, anti-psychotic drug induced weight gain, obesity, post-traumatic stress disorder and alcoholism.

Accordingly, the invention relates to compounds according to the first aspect, and pharmaceutically acceptable salts thereof, and pharmaceutical compositions comprising such compounds and / or salts thereof for use in the treatment of pathological conditions or disease of human body which comprises administering to a subject in need of said treatment, an effective amount of the dihydropyridine derivatives of the invention or a pharmaceutically acceptable salt thereof.

As used herein, the term halogen atom is used to designate an atom selected from the group consisting of chlorine, fluorine, bromine or iodine atom, preferably bromine, fluorine or chlorine atom.

As used herein the term alkyl is used to designate linear or branched hydrocarbon radicals (CnH2n+1) having 1 to 12 carbon atoms. Examples include methyl, ethyl, n-propyl, i-propyl, n-butyl, sec-butyl, tert-butyl, n-pentyl, 1-methyl-butyl, 2-methyl-butyl, isopentyl, 1-ethylpropyl, 1,1-dimethylpropyl, 1,2-dimethylpropyl, n-hexyl, 1-ethylbutyl, 2-ethylbutyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 1,3dimethylbutyl, 2,2-dimethylbutyl, 2,3-dimethylbutyl, 2-methylpentyl and 3-methylpentyl, n-heptyl, 3-methylheptyl, n-octyl, 2,2-dimethyloctyl radicals. In a preferred embodiment said alkyl groups have 1 to 6 carbon atoms.

As used herein, the term haloalkyl is used to designate C1-C6 alkyl substituted by one or more halogen atoms, preferably one, two or three halogen atoms. The haloalkyl groups may be linear or branched. Preferably, the halogen atoms are selected from the group consisting of fluorine or chlorine atoms. In a preferred embodiment, the haloalkyl group is a linear or branched C1-C4 alkyl substituted by one, two or three fluorine or chlorine atoms.

As used herein, the term cycloalkyl is used to designate hydrocarbon cyclic groups (CnH2n-1) having 3 to 6 carbon atoms. Such cycloalkyl groups include, by way of example, single ring structures such as cyclopropyl, cyclobutyl, cyclopentyl, cyclooctyl, and the like.

As used herein, the term heterocyclyl is used to designate saturated rings comprising 3 to 6 carbon atoms and 1, 2 or 3 heteroatoms selected from O, S and N as part of the ring. The heterocyclyl groups include, for example, azetidinyl, pyrrolidinyl, piperidinyl, morpholinyl and piperazinyl.

As used herein, the term C1-C6 alkoxy is used to designate radicals which contain a linear or branched C1-C6 alkyl group linked to an oxygen atom (CnH2n+1-O-). Preferred alkoxy radicals include methoxy, ethoxy, n-propoxy, i-propoxy, n-butoxy, sec-butoxy, t-butoxy, trifluoromethoxy, difluoromethoxy, hydroxymethoxy, 2-hydroxyethoxy or 2-hydroxypropoxy.

As used herein, the term carbonyl group is used to designate a group C=O. As used herein, when the term oxo (=O) is used to designate a subsitutent in a ring, it means that a carbon atom of said ring is present in the form of a carbonyl (C=O) group.

As used herein, the term heteroaryl group is used to designate a 5 or 6-membered heteroaromatic ring containing carbon, hydrogen and one or more atoms selected from O, N and S. Said groups may optionally be substituted by 1, 2 or 3 substituents selected from the group consisting of halogen atom, cyano group, linear or branched C1-C6 haloalkyl, linear or branched C1-C6 alkyl, hydroxy, linear or branched C1-C6 alkoxy, amino, alkylamino and dialkylamino. The heteroaryl groups may optionally be substituted by substituents such as those defined under R6 and B1. The preferred groups are optionally substituted pyridyl and pyrimidinyl. When a heteroaryl radical carries 2 or more substituents, the substituents may be the same or different.

As used herein, the term alkylamino is used to designate radicals which contain a linear or branched C1-C6 alkyl group linked to a group -NH-. Preferred alkylamino radicals include methylamino, ethylamino, n-propylamino, i-propylamino, n-butylamino, sec-butylamino, t-butylamino, trifluoromethylamino, difluoromethylamino, hydroxymethylamino, 2-hydroxyetylamino or 2-hydroxypropylamino.

As used herein, the term dialkylamino is used to designate radicals which contain two linear or branched C1-C6 alkyl groups linked to a group nitrogen atom which alkyl groups may be identical or different. Preferred dialkylamino radicals include di(methyl)amino, di(ethyl)amino, di-(n-propyl)amino, di-(i-propyl)amino, di-(n-butyl)amino, di-(sec-butyl)amino, di-(t-butyl)amino, di-(trifluoromethyl)amino, di(hydroxymethyl)amino,di(2-hydroxyetyl)amino, di(2-hydroxypropyl)amino, methylethylamino, methyl-i-propylamino and ethyl-n-propylamino.

As used herein, some of the atoms, radicals, chains or cycles present in the general structures of the invention are "optionally substituted". This means that these atoms, radicals, chains or cycles can be either unsubstituted or substituted in any position by one or more, for example 1, 2, 3 or 4, substituents, whereby the hydrogen atoms bound to the unsubstituted atoms, radicals, chains or cycles are replaced by chemically acceptable atoms, radicals, chains or cycles. When two or more substituents are present, each substituent may be the same or different.

As used herein, the term pharmaceutically acceptable salt is used to designate salts with a pharmaceutically acceptable acid or base. Pharmaceutically acceptable acids include both inorganic acids, for example hydrochloric, sulphuric, phosphoric, diphosphoric, hydrobromic, hydroiodic and nitric acid and organic acids, for example citric, fumaric, maleic, malic, mandelic, ascorbic, oxalic, succinic, tartaric, benzoic, acetic, methanesulphonic, ethanesulphonic, benzenesulphonic or p-toluenesulphonic acid. Pharmaceutically acceptable bases include alkali metal (e.g. sodium or potassium), alkali earth metal (e.g. calcium or magnesium) hydroxides, and organic bases, for example alkyl amines, phenylalkyl amines and heterocyclic amines.

Other preferred salts according to the invention are quaternary ammonium compounds wherein an equivalent of an anion (X-n), wherein "-n" indicates the negative charge of the anion and is typically -1, -2 or -3, is associated with the positive charge of the N atom. X-n may be an anion of various mineral acids such as, for example, chloride, bromide, iodide, sulphate, nitrate, phosphate, or an anion of an organic acid such as, for example, acetate, maleate, fumarate, citrate, oxalate, succinate, tartrate, malate, mandelate, trifluoroacetate, methanesulphonate and ptoluenesulphonate. X-n is preferably an anion selected from chloride, bromide, iodide, sulphate, nitrate, acetate, maleate, oxalate, succinate or trifluoroacetate. More preferably, X- is chloride, bromide, trifluoroacetate or methanesulphonate.

According to one embodiment of the present invention in the compounds of formula (I), X1, X2, X3 and X5 represent C-H or C-B1 group. In a preferred embodiment B1 represents a halogen atom. In a more preferred embodiment X1, X3 and X5 represent C-H and X2 is C-B1, wherein B1 represents a halogen atom.

According to another embodiment of the present invention in the compounds of formula (I), X4 represents a group selected from C-B1 and N atom. In a preferred embodiment X4 represents C-B1. In a more preferred embodiment B1 is selected from -CN group and halogen atom.

According to another embodiment of the present invention in the compounds of formula (I), R1 is a group selected from -COR5, -COOR 5 and -CN group. In a preferred embodiment, R5 is independently selected from C3-C5 cycloalkyl, -C1-C3 alkyl wherein the terminal methyl is unsubstituted or substituted by three fluorine atoms (-CF3) and C1-C3 alkyl optionally substituted at any position by an alkynyl group. In a more preferred embodiment, each R5 is independently selected from -C1-C3 alkyl and -C3-C5 cycloalkyl.

According to one embodiment of the present invention in the compounds of formula (I), R2 is a group selected from -COR8 and -COOR8 preferably -COOR8. In a preferred embodiment, R8 is independently selected from:

In a more preferred embodiment R2 represents -COOR8, wherein R8 represents independently:

According to another embodiment of the present invention in the compounds of formula (I), R2 represents a -CN group.

According to another embodiment of the present invention in the compounds of formula (I), R3 is a group selected from C3-C6 cycloalkyl optionally substituted by 1, 2 or 3 halogen atoms; and linear or branched C1-C4 alkyl optionally substituted by 1, 2 or 3 substituents selected from halogen atom, -N(R6)R7, and -OR6. In a preferred embodiment, R6 and R7 are selected from hydrogen atom and a linear or branched C1-C6 alkyl.

In a more preferred embodiment R3 is selected from linear or branched C1-C4 alkyl and C3-C6 cycloalkyl. In a more preferred embodiment R3 is selected from methyl or cyclopropyl group, said groups being optionally substituted by 1, 2 or 3 fluorine atoms.

According to another embodiment of the present invention in the compounds of formula (I), R4 represents a linear or branched C1-C6 alkyl optionally substituted by 1, 2 or 3 substituents selected from A1 or B2. In a more preferred embodiment A1 represents C3-C6 cycloalkyl and B2 represents a group selected from halogen atom, -N(R6)R7, and-OR6. In a more preferred embodiment, R6 and R7 are selected from hydrogen atom and a linear or branched C1-C6 alkyl. In a more preferred embodiment R4 represents a linear or branched C1-C3 alkyl optionally substituted by 1, 2 or 3 substituents selected from 1, 2 or 3 fluorine atoms and hydroxyl group.

According to another embodiment of the present invention in the compounds of formula (I), R4 represents an A1 group. In a more preferred embodiment, A1 represents C3-C6 cycloalkyl which optionally contains 1, 2 or 3 heteroatoms selected from O, S and N, and which ring is optionally substituted by C1-C3 alkyl. In a more preferred embodiment R4 represents an A1 group, which represents C3-C6 cycloalkyl.

According to one embodiment of the present invention in the compounds of formula (I), R4 represents a -N(R6)R7 group. In a more preferred embodiment, R6 and R7 are selected from a hydrogen atom and a linear or branched C1-C4 alkyl.

In a more preferred embodiment R4 is a group selected from:

According to one embodiment of the present invention in the compounds of formula (I), X1, X2, X3 and X5 represent -CH, X4 represents C-B1, wherein B1 represents -CN group or bromine atom, R1 is a group selected from -C(O)CH3, -C(O)OCH3, -C(O)OCH2-alkynyl and CN, R2 is a group selected from -C(O)OCH-lineal or branched C1-C5 alkyl group optionally substituted by 1, 2 or 3 fluorine atoms and C(O)OCH2-cyclopropyl optionally substituted by 1, 2 or 3 fluorine atoms, R3 is a group selected from linear or branched C1-C3 alkyl and C3-C4 cycloalkyl and R4 is a group selected from a linear or branched C1-C3 alkyl, C3-C4 cycloalkyl and -NH2.

Compound according to claim 1 wherein X1, X2, X3 and X5 represent a -CH, X4 represents a C-B1, wherein B1 represents -CN group, R1 is a group selected from - C(O)CH3, -C(O)OCH3 , R2 is a group selected from -C(O)OCH2 -cyclopropyl optionally substituted by 1, 2 or 3 fluorine atoms, and -C(O)OCH2-CF3 , R3 is a group selected from methyl and cyclopropyl and R4 is a group selected from cyclopropyl and -NH2.

According to another embodiment of the present invention in the compounds of formula (I), X1, X3, X4 and X5 represent a -CH and X2 represents a C-B1 group. In a more preferred embodiment B1 represents a halogen atom.

According to another embodiment of the present invention in the compounds of formula (I), X1, X2, X3, X4 and X5 represent a -CH.

Individual compounds of the present invention include:

When the compounds of the present invention are combined with other therapeutic agents, said other therapeutic agents are selected from the group consisting of:

Examples of gonadotropin-releasing hormone (GnRH) receptor agonists include, but are not limited to leuprolide, goserelin, and buserelin. Examples of gonadotropin-releasing hormone (GnRH) receptor antagonist include, but are not limited to degarelix and abarelix, among others.

Examples of androgen receptor antagonists include but are not limited to nilutamide, bicalutamide, flutamide and enzalutamide.

Examples of CYP17 inhibitors include but are not limited to abiraterone and gleterone.

Examples of VEGF receptor inhibitors include, but are not limited to bevacizumab, axitinib and brivanib alaninate.

Examples of EGFR inhibitors include, but are not limited to gefitinib, afatinib, cetuximab and panitumumab.

Examples of PI3K inhibitors include, but are not limited to pictilisib (also known as GDC 0941) and BKM120.

Examples of mTOR inhibitors include, but are not limited to temsirolimus, ridaforolimus and everolimus.

Examples of dual PI3K and mTOR inhibitors include, but are not limited to Apitolisib (GDC-0980), dactolisib (BEZ235) and LY3023414.

Examples of AKT inhibitors include, but are not limited to A-443654, perifosine and ipatasertib.

Examples of c-Met inhibitors include, but are not limited to tivantinib, JNJ-38877605, cabozantinib and capmatinib.

Examples of Src blockers include, but are not limited to dasatinib, saracatinib, bosutinib, and KX01, which are in clinical development.

Examples of PARP inhibitors include, but are not limited to olaparib and veliparib.

Examples of ALK inhibitors include, but are not limited to X-396, alectinib, ceritinib, lorlatinib and crizotinib (dual ALK and ROS-1 inhibitor).

Examples of anti-IGF antibodies include, but are not limited to figitumumab, ganitumab and BI836845.

Examples of taxane anti-neoplastic agents include, but are not limited to cabazitaxel and larotaxel.

Examples of topoisomerase II inhibitors include, but are not limited to etoposide and teniposide.

Examples of anti-tumor antibiotics include, but are not limited to doxorubicin, bleomycin, daunorubicin, daunorubicin liposomal, mitoxantrone, epirubicin, idarubicin, and mitomycin C.

Examples of HSP90 (heat shock protein 90) inhibitors include, but are not limited to Debio 0932, MPC-3100, IPI-504, NVP-AUY922.

Examples of Aurora kinase inhibitors include, but are not limited to alisertib, tozasertib, danusertib and barasertib.

Examples of PSA-directed vaccine include, but are not limited to ProstVac-VF.

The compounds of this invention can be prepared by using the procedures described below. To facilitate the description of the procedures, concrete examples have been used in some cases, however they do not restrict in any way the scope of the present invention.

When one of X1, X2, X3, X4 and X5 represents C-B1 and the rest of X1, X2, X3, X4 and X5 represent C-H the compounds of formula (I) are benzothiophenes of formula (1-a3), as depicted in Scheme 1-a and can be obtained be reacting thiophenol precursor (1-a1), where B1 is selected from halogen, alkyl, haloalkyl, cycloalkyl, heterocycloalkyl and aryl, with 2-bromo-1,1-diethoxyethane and potassium carbonate in acetone affording intermediates (1-a2) which are cyclized to benzothiophenes (1-a3) by heating with polyphosphoric acid in toluene.

When one of X1, X2, X3, X4 and X5 represents C-B1 and the rest of X1, X2, X3, X4 and X5 represent C-H the compounds of formula (I) are benzothiophene intermediates of formula (III), as depicted in Scheme 1-b, can be obtained from halogen precursor (II), where halogen (Hal) is selected from chloro, iodo or preferentially bromo.

More specifically, when B1 represents -C(O)CF3, one way to synthesize benzothiophene derivatives of formula (III) is by reacting the halogen precursor (II), specifically bromo precursor, with n-butyllithium and 2,2,2-trifluoro-1-morpholinoethan-1-one in diethyl ether at -60°C.

When B1 represents -CN, the cyano group can be introduced by heating the halogen precursor (II), specifically bromo precursor, with zinc cyanide and tetrakis(triphenylphosphine)palladium(0) in a sealed tube at 90°C. The cyano derivative (III) can be converted into the carboxylic acid by heating with potassium hydroxide in methanol and water at 80°C, which can be further transformed into the methyl ester by refluxing in saturated HCI in methanol.

When B1 represents mono- or dialkylamino, one way to synthesize benzothiophene derivatives of formula (III) is by refluxing the halogen precursor (II), specifically bromo precursor, with the corresponding amine, Pd2(dba)3, tricyclohexylphosphine and NaOtBu in dry toluene.

When B1 represents mono- or dialkylaminocarbonyl, benzothiophene intermediates of formula (V) can be obtained by reacting carboxylic acid precursor (IV) with the corresponding amine, HOBT and DIPEA in DMF at room temperature, as depicted in Scheme 2.

3-formylbenzo[b]thiophenes of formula (VII) can be obtained by reacting precursor (VI) with dichloro(methoxy)methane and titanium tetrachloride in dichloroethane at room temperature as represented in reaction Scheme 3.

Another way of introducing a formyl group in position 3 of the bicyclic heterocycle (X), as depicted in reaction Scheme 4, is by heating the 3-bromo derivative (VIII) with copper(l) cyanide in NMP at 200°C and subsequent reduction with DIBAL in toluene.

One way to synthesize dicarbonyl intermediates of formula (XI) and (XII) (used in the synthesis of compounds of formula (I) wherein R2 represents -COOR8 or - C(O)N(R 8)R9) is by heating commercially available 2,2,6-trimethyl-4H-1,3-dioxin-4-one and the corresponding alcohol or amine in toluene at 150 °C, as represented by the acetoacetylation reaction depicted in reaction Scheme 5.

One way to synthetize dicarbonyl intermediates (XIV) (used in the synthesis of compounds of formula (I) wherein R4 represents alkyl, cycloalkyl, phenyl or heteroaryl) is by reesterification in which methyl ester (XIII) and an alcohol are refluxed in dry toluene using a Dean-Stark trap, as shown in Scheme 6.

Another reesterification method affords dicarbonyl intermediates (XIV) by refluxing methyl ester (XIII) and an alcohol in dry toluene in a sealed tube using 10 mol-% trichlorobismuthane and molecular sieves, as represented in Scheme 7.

Dicarbonyl intermediates of formula (XIII) and (XVII) (used in the synthesis of compounds of formula (I) wherein R2 represents -COR8 or -COOR8) can be synthesized via Claysen condensation by treating a methylketone derivative (XV) or acetone with potassium tert-butoxide in THF and reacting the mixture with either dimethyl carbonate or a methyl or ethyl ester (XVI), as represented in reaction Scheme 8.

Ketoester intermediates of formula (XVIII) (used in the synthesis of compounds of formula (I) wherein R2 represents -COOR8 and R4 represent -CH2OR6 or-CH2N(R6)R7) can be synthesized by deprotonating an alcohol with sodium hydride in THF and treating the mixture with methyl 4-chloro-3-oxobutanoate, as represented in reaction Scheme 9. Ketoester intermediates of formula (XIX) can be synthesized accordingly by heating methyl 4-chloro-3-oxobutanoate with an amine in acetone and trimethylamine.

1,3-ketoester intermediates of formula (XIV) can be transformed into enamines (XX) either by heating with ammonium acetate, acetic acid and molecular sieves in THF or by reacting with ammonium bicarbonate in methanol, as represented in reaction Scheme 10. Accordingly, 1,3-diketones (XXI) can be converted into a mixture of enamines (XXII) and (XXIII) using the procedure described above or, alternatively, by reaction in aqueous ammonia.

Knoevenagel products of formula (XXIV) and (XXVI) can be prepared by reacting aldehydes (X) (e.g. benzo[b]thiophene-3-carbaldehyde) and 1,3-dicarbonyl intermediates (XIV) or (XXV), respectively, with acetic acid, piperidine and molecular sieves in toluene in the microwave oven, as adapted from Liu & Zhang, Angew. Chem. Int. Ed. 2009, 48, 6093-6 and represented in reaction Scheme 11.

Dihydropyridines of formula (XXVII) and (XXVIII) can be prepared in a 3-component Hantzsch synthesis by heating Knoevenagel products (XXVI) with 1,3-dicarbonyl compounds (XIV), (XXI) or (XXIX) and ammonia in alcohols such as e.g. isopropanol, ethanol or 2,2,2-trifluoroethanol, as represented in reaction Scheme 12. Instead of ammonia, NH4OAc in MeOH under microwave conditions can be used to synthesize dihydropyridines of formula (XXVII), (XXVIII) and (XXX).

Another way of preparing dihydropyridines (XXXI) and (XXXIII) is by heating Knoevenagel products (XXVI) or (XXIV) with enamines (XXII) or (XXXII), respectively, in methanol under microwave conditions (Scheme 13).

Dihydropyridines of formula (XXXIII) can also be prepared by heating Knoevenagel products (XXVI) and enamines (XX) in AcOH, as represented in reaction Scheme 14.

Dihydropyridines of formula (XXXV) (which are used as intermediates in the synthesis of compounds of formula (I) wherein R4 represents -NH2) can be synthesized by heating Knoevenagel product (XXVI) and amidine (XXXIV) with piperidine in isopropanol, as represented in reaction Scheme 15.

The dihydropyridine scaffold can be assembled without prior formation of a Knoevenagel product. For example, compounds of formula (I) in which R1 and R2 both represent -CN, can be synthesized by heating aldehyde (X) and enamines (XXXVIa) and (XXXVIb) with acetic acid in isopropanol, as represented in reaction Scheme 16. When R3 is not equal to R4, mixtures of dihydropyridines (XXXVIIa), (XXXVIIb) and (XXXVIIc) are obtained that need to be separated, e.g. by column chromatography.

In a variation of the method described above, one of the enamines, e.g. (XXXVIb), can be replaced by a ketone, e.g. (XXXVIc), to prepare the same mixture of dihydropyridines (XXXVIIa), (XXXVIIb) and (XXXVIIc).

The reaction conditions described above in Scheme 16 (heating with acetic acid in alcohols such as e.g. isopropanol, optionally in the presence of substoichiometric amounts of piperidine) can be utilized in a wider scope to synthesize dihydropyridines of formula (I), in which R1 represents either -CHO, -COR5 or -CN and R2 is selected from -COR8, -S(O)nR8 (wherein n is 1 or 2), -COOR8 and -C(O)N(R8)R9, provided that an aldehyde (X) (e.g. benzo[b]thiophene-3-carbaldehyde) is reacted with:

  1. (a) two enamines as exemplified by, but not limited to, the compounds of formulae (XX), (XXII), (XXIII), (XXXII) or (XXXVIa/b), or
  2. (b) one enamine as exemplified by, but not limited to, the compounds of formulae (XX), (XXII), (XXIII), (XXXII) or (XXXVIa/b) and a 1,3-dicarbonyl compound as exemplified by, but not limited to, the compounds of formulae (XIV), (XXV) or (XXIX).

The enamines described above can also be formed in situ giving rise to yet another variation of the Hantzsch synthesis of the dihydropyridine scaffold in which compounds of formula (I) are prepared in a 4-component reaction by heating an aldehyde (e.g. benzo[b]thiophene-3-carbaldehyde), two 1,3-dicarbonyl compounds (e.g. compounds of formula (XIV), (XXV) or (XXIX)) and aqueous ammonia in alcohols (e.g. ethanol).

The methods for synthesizing the 1,4-dihydropyridine scaffold, as described above, are variations of the well documented Hantzsch Reaction that has been reviewed, for example, in "Hantzsch reaction: Recent advances in Hantzsch 1,4-dihydropyridines", A. Saini et al., J Scient Indust Res 2008, 67, 95-111.

Dihydropyridines of formula (I) bearing halogen atoms, such as -Br, on an aromatic ring, can be further derivatized by metal-catalyzed coupling reactions in which the halogen atom is replaced by e.g. aryl, heteroaryl or small alkyl groups (Suzuki coupling), by amines (Buchwald coupling) or by a cyano group (Zn coupling).

For example, halogen-substituted benzyl esters of formula (XXXVIII) can be coupled with pyridinylboronic acid (or other heteroaryl, phenyl or alkyl boronic acids) by heating both components with a palladium catalyst, e.g. palladium-tetrakis(triphenylphosphine), and a base, e.g. potassium carbonate, in a 3:1 mixture of DME and water to afford dihydropyridine (XXXIX) as represented in Scheme 17.

In another example, benzothiophenes of formula (XL) that are substitued with halogen atoms as depicted in Scheme 18, can be converted into compounds of formula (XLI) by Suzuki coupling with pyridylboronic acid utilizing a catalyst (e.g. Pd(dppf)Cl2∗DCM) and aqueous CS2CO3 in dichloromethane at reflux temperature. In yet another example, coupling of halogen-substituted benzothiophenes (XL) with stannanes, like e.g. tributyl(phenyl) stannane, using catalysts such as palladium(ll)bis(triphenylphosphine)dichloride in hot toluene (2 mL) afford compounds of formula (XLII).

The bromine atom of benzothiophenes (XL) shown in Scheme 18 can be displaced by a cyano group by heating with Zn(CN)2 and Pd(PPh3)4 in DMF to afford dihydropyridines of formula (XLIII).

Pharmacological activity Rat androgen receptor binding

The assay was carried out following the instructions of polarscreen AR competitor assay green kit (Life Technologies, Cat#.: A15880). Briefly, the test is based on measuring the changes of fluorescence polarization shown by the Fluormone AL Green. Thus, when the Fluormone is bound to the receptor, a fluorescence polarization is observed that diminishes when a ligand competes for the receptor binding site and the Fluormone gets displaced.

The inhibition values for each of the compounds are measured in duplicate at 1 and 10 µM) and the plate is incubated for 4 hours at RT. Fluorescence polarization is the measured in a Tecan Infinite M1000 Pro reader (Aexc=485 nm, λem=535 nm). In order to obtain a dose-response curve and calculate the potency of inhibition (expressed as IC50) for AR, a series of 1:10 dilutions in test in 1 X buffer from 10 µM) to 0.1 nM for each compound was carried out.

Human androgen receptor binding

Radioligand binding studies were carried out on endogenously expressed human androgen receptor using the LnCap prostate cancer cell line. The test is based on measuring radioactively labelled AR ligand [3H]-methyltrienolone that gets displaced by increasing concentrations of test compound. The test is performed in 96-well plates (Falcon, #353072). Positive and negative controls are also required in each analyzed plate to evaluate the total and non-specific binding binding of the radioligand to the receptor. Cells (60000 per well) were incubated for 24 hours before executing the experiment at 37°C and in a 5% CO2 atmosphere. Medium was replaced by assay buffer (RPMI-640 (ATCC #30-2001) supplemented with 0.1% bovine serum albumin (Sigma #8806-56) and Triamcinolone acetonide (Sigma #T6501)). The compounds at the desired concentrations and [3H]methyltrienolone (Perkin Elmer #NET590) were added to the wells. <b>Table 1: Test conditions</b> Sample Non-specific binding Total binding Assay buffer 80 µL 80 µL 90 µL Compound (10x) 10 µL [3H]methyltrienolone (20 nM) 10 µL 10 µL 10 µL Testosterone (200 µM) 10 µL

In order to obtain a dose-response curve and calculate the potency of inhibition (expressed as IC50) for AR, a series of 1:10 dilutions in test in 1 X buffer from 10 µM) to 0.1 nM for each compound was carried out.

Cells were incubated for 2 hours at 37°C in a 5% CO2 atmosphere and then washed twice with ice-cold HBSS. Then cells were lysed with solubilization buffer (Hank's Balanced Salt Solution (HBSS, Sigma # H6648), 0.5% Sodium dodecyl sulfate 0.5% (SDS, Sigma #L7390-500G, Lote: 078K0102) and 20% Glycerol (Sigma Aldrich, #G2025) for 30 minutes at RT. 75 µL aliquots were taken from each well and transferred to a 96-well flexiplate (Perkin Elmer #1450-401) and mixed with 100 µL of scintillation cocktail (Optiphase supermix, PerkinElmer, # 1200-439). Plates are shaken for 60 minutes and radioactivity was measured in a beta scintillation counter (Microbeta Trilux, Perkin Elmer).

Competition binding in IM-9 human glucocorticoid receptor (GR IM-9)

Glucocorticoid receptor competition binding assays were carried out in a polypropilene 96-well plate. In each well was incubated 100 µg of cytosol from IM-9 cell line, 1.5 nM [3H]-Dexamethasone (71 Ci/mmol, 1 mCi/ml, Perkin Elmer NET1192001MC) and compounds studied and standard. Non-specific binding was determined in the presence of Triamcinolone 10µM (Sigma Aldrich T6376).

The reaction mixture (Vt: 200 µl/well) was incubated at 4°C for 6 hours, 180 µL was transfered to GF/B 96-well plate (Millipore, Madrid, Spain) pretreated with 0.5% of PEI and treated with binding buffer (TES 10mM, sodium molybdate 10mM, EDTA 1 mM, pH 7.4, 2-mercaptoethanol 20 mM,Glycerol 10%) after was filtered and washed six times with 250 µl wash buffer (TES 10mM, sodium molybdate 10mM, EDTA 1 mM, pH 7.4, 2-mercaptoethanol 20 mM ,Glycerol 10%), before measuring in a microplate beta scintillation counter (Microbeta Trilux, PerkinElmer, Madrid, Spain).

Functional Assay AR-receptor

MDA-MB-453 cells were cultured in DMEM (ATCC, ref 30-2002), 10% FBS, 1% penicillin, 1% streptomycin, 75 µg/ml gentamycin.

The day before performing assay, the cells were trypsinized and seeded in a 384-well plate (Greiner, ref 781098) (8000 cells/well) in 50 µl of complete culture medium. Cells were stores at 37°C in a 5% CO2 atmosphere overnight.

The medium was replaced by 50 µl complete growth medium with 0.1% FBS. Compounds were dispensed with Echo 550 (Labcyte) and incubated for 24 h at 37°C in a 5% CO2 atmosphere. Antagonist effect was measured in the presence of DHT 1nM.

After incubation, assay medium removed and 20 µl of 1× Promega lysis buffer were added to each well. The plate was incubated 15 minutes at RT and quick-frozen to -80°C and the thaw to room temperature. 50 µl of luciferase substrate (E4550, Promega) were added to each well and luminescence (integration time 1000 msec) was read in an EnSpire multilabel reader (Perkin Elmer).

Results

Table shows the binding of some compounds of the present invention in the differents assays. Example AR rat-fluorimetric AR LNCaP GR IM-9 AR antag KB A1 A A2 B A3 A A10 A B6 B C B B B12 C C A B18 A B21 B C B C1 B C2 B C B B C3 A C6 B B A C7 B C10 C B C14 B A B C19 C C20 B B C21 A C23 A C24 A A A C C26 A C27 C C28 B C29 A C32 C C34 C A C35 B A C36 B A C C37 A A C38 A C39 B A C C41 C B B C42 C A A C43 C B A C44 C B B C45 C C46 B A B C47 B A C48 C B B C52 B B C53 A C56 B A C57 B C58 A A B C60 B C61 C B C62 B C64 C B C65 C A B C66 B A A C67 A A A C68 C C71 C A C C72 C A C C73 C A C C74 B C C75 B C C76 C A C77 B C78 C B C79 A C C80 C C C81 A C82 A C83 B C84 A C85 C C86 B C91 A C92 A C93 B C94 A C95 A C96 B C97 A C98 B C99 B C100 A C101 A C102 B C103 A C104 A C105 B C106 B C107 A C C108 A C C109 A C110 A C111 A C112 B A C113 B A C114 C C115 C C116 B C117 B A B C118 A A B C119 C A B C120 B A C121 C B C122 B A C123 A A D1 A B C D2 C D3 A D5 A D6 B C D7 A C D8 A D10 A D11 A D12 A D13 A D14 C D15 B D16 A D17 A D18 A D19 A D20 B D21 A D22 C A D23 B A D24 B A B D25 C A D26 B B D27 B A B D28 B D29 B A D30 B A B D31 B A B D32 B A B D33 B A D34 B A D35 B B D36 B A D37 B A B D38 B A D39 B A B D40 B A D41 B A A D42 B A D43 B A C D44 A D45 B A D46 C A C D47 B A C D48 A D49 A A D50 A D51 A D52 A D53 A D54 A D55 A D56 A E1 A A G1 A A G2 C G3 C G4 C H1 A H2 A H3 C H4 C H5 C H6 C H7 C H8 C H9 C H10 C H11 C H12 C H13 C H14 C H15 A B I2 B B J1 A J2 A J3 B J6 C J10 B J11 A J12 A J13 C J14 C J15 C J16 C J17 C J18 C J19 C J20 B J21 A A J22 A A A J23 A A B C J24 C J25 B J25-a B J26 A A B J27 C C J28 A A J28-a B A J29 B J30 C J31 C A J32 C B J33 B B J34 B J36 C J38 B J40 C J41 A A B J42 B A A J42-a B J43 A B K1 B K2 C L1 C A B L2 C A B L3 A A B L4 A A B L5 A C L7 B C L8 C L9 B L12 B L13 B L14 C M1 C N1 C

Range:

  1. A: IC50 < 100 nM
  2. B: 100 nM =< IC50 < 1 µM
  3. C: IC50 >= 1 µM

As can be seen from the results described in Table 1, the compounds of the present invention are modulators of nuclear receptor selected from androgen receptor and glucocorticoid receptor.

The invention thus also relates to a compound as defined in the first aspect for use in the treatment or prevention of diseases known to be susceptible to improvement by treatment with a modulator of androgen receptor and glucocorticoid receptor said diseases being selected from prostate cancer, castration-resistant prostate cancer, pancreatic cancer, bladder cancer, renal cancer, gastric cancer, lung cancer, breast cancer, colon cancer, colorectal cancer, ovarian cancer, and other solid tumours, melanoma, metastasizing cancers, benign prostate hyperplasia, polycystic ovary syndrome (PCOS), hair loss, hirsutism, acne, hypogonadism, muscle wasting diseases and cachexia, and Cushing's syndrome, anti-psychotic drug induced weight gain, obesity, post-traumatic stress disorder and alcoholism. Accordingly, the invention also relates to the derivatives of the first aspect and pharmaceutically acceptable salts thereof, and pharmaceutical compositions comprising such compounds and/or salts thereof, for use in the treatment of disorders of the human body which comprises administering to a subject requiring such treatment an effective amount of the dihydropyridine derivative of the invention or a pharmaceutically acceptable salt thereof.

The present invention also provides pharmaceutical compositions which comprise, as an active ingredient, at least a dihydropyridine derivative of formula (I) or a pharmaceutically acceptable salt thereof in association with other therapeutics agents, as have been mentioned above, and with a pharmaceutically acceptable excipient such as a carrier or diluent. The active ingredient may comprise 0.001% to 99% by weight, preferably 0.01% to 90% by weight of the composition depending upon the nature of the formulation and whether further dilution is to be made prior to application. Preferably, the compositions are made up in a form suitable for oral, topical, nasal, rectal, percutaneous or injectable administration.

The pharmaceutically acceptable excipients, which are admixed with the active compound or salts of such compound, to form the compositions of this invention, are well known per se and the actual excipients used depend inter alia on the intended method of administering the compositions.

Compositions of this invention are preferably adapted for injectable and oral (per os) administration. In this case, the compositions for oral administration may take the form of tablets, sustained release tablets, sublingual tablets, capsules, inhalation aerosols, inhalation solutions, dry powder inhalation, or liquid preparations, such as mixtures, elixirs, syrups or suspensions, all containing the compound of the invention; such preparations may be made by methods well-known in the art.

The diluents, which may be used in the preparation of the compositions, include those liquid and solid diluents, which are compatible with the active ingredient, together with colouring or flavouring agents, if desired. Tablets or capsules may conveniently contain between 2 and 500 mg of active ingredient or the equivalent amount of a salt thereof.

The liquid composition adapted for oral use may be in the form of solutions or suspensions. The solutions may be aqueous solutions of a soluble salt or other derivative of the active compound in association with, for example, sucrose to form syrup. The suspensions may comprise an insoluble active compound of the invention or a pharmaceutically acceptable salt thereof in association with water, together with a suspending agent or flavouring agent.

Compositions for parenteral injection may be prepared from soluble salts, which may or may not be freeze-dried and which may be dissolved in pyrogen free aqueous media or other appropriate parenteral injection fluid.

Effective doses are normally in the range of 2-2000 mg of active ingredient per day. Daily dosage may be administered in one or more treatments, preferably from 1 to 4 treatments, per day.

The present invention will be further illustrated by the following examples. The following are given by way of illustration and do not limit the scope of the invention in any way. The synthesis of the compounds of the invention is illustrated by the following examples including the preparation of the intermediates, which do not limit the scope of the invention in any way.

EXAMPLES General

Reagents, solvents and starting products were acquired from commercial sources and used without further purification. All reactions were performed under an atmosphere of air and with anhydrous solvents unless otherwise stated. The term "concentration" refers to the vacuum evaporation using a Büchi rotavapor. When indicated, the reaction products were purified by "flash" chromatography on silica gel (40-63 µm) with the indicated solvent system. Yields refer to isolated compounds estimated to be > 95% pure as determined by 1H NMR. All the dihydropyridines were obtained as racemic mixtures.

Mass spectrometry was carried out on a Varian MAT-711 spectrometer employing EI and ESI methods. HPLC experiments were carried out on a LaChrom Elite L-2350 instrument (Sunfire silica gel column 5 µm, 4.6 mmx150 mm) equipped with an L-2455 diode array detector. HPLC-MS analysis was performed on a Gilson instrument equipped with a Gilson 321 piston pump, a Gilson 864 vacuum degasser, a Gilson 189 injection module, a 1/1000 Gilson splitter, a Gilson 307 pump, a Gilson 170 detector, and a Thermoquest Fennigan aQa detector.

1H and 13C nuclear magnetic resonance experiments were carried out using either a Varian Inova 500 MHz or Varian Mercury 300 or 400 MHz NMR spectrometers. Chemical shifts are reported relative to the deuterated solvent signal or tetramethylsilane as an internal reference. Coupling constants J are given in Hertz (Hz) and multiplicities as follows: s = singlet, d = doublet, t = triplet, q = quartet, m = multiplet or as a combination of them.

PREPARATION OF INTERMEDIATES General procedure aa Intermediate 1: 7-chlorobenzo[b]thiophene

To a solution of 2-chlorobenzenethiol (1.53 g, 10.58 mmol, 1.0 eq.) and 2-bromo-1,1-diethoxyethane (2.4 mL, 15.87 mmol, 1.5 eq.) in acetone (10 ml) was added potassium carbonate (3.65 g, 26.45 mmol, 2.5 eq). The mixture was refluxed for 90 minutes and then 0,5 eq of 2-bromo-1,1-diethoxyethanewas added. The mixture was refluxed for another 2 hours, cooled to room temperature and concentrated. The resulting residue was purified by flash column chromatography on silica gel using hexane/diethyl ether (2%) as eluent to afford (2-chlorophenyl)(2,2-diethoxyethyl)sulfane as a light yellow oil (2.71 g, 97%).

To a solution of polyphosphoric acid (6.0 g, 51.97 mmol, 9 eq.) in toluene (15 ml) was added under vigorous stirring a solution of (2-chlorophenyl)(2,2-diethoxyethyl)sulfane (1.5 g, 5.77 mmol, 1 eq) in toluene (5 mL). The mixture was stirred at reflux temperature for 5 hours and was then poured into ice cold water (30 mL) and stirred for 10 minutes. The mixture was washed with toluene, sat. aq. bicarbonate solution and brine, dried (Na2SO4) and concentrated. The resulting residue was purified by flash column chromatography on silica gel using hexane as eluent (as described in WO 2009/008992 A2) to afford 7-chlorobenzo[b]thiophene as a light-yellow oil (0.68 g, 70%).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 7.77 - 7.71 (m, 1H), 7.50 (d, J = 5.4 Hz, 1H), 7.41 - 7.30 (m, 3H).

Intermediate 2: 7-(trifluoromethyl)benzo[b]thiophene

General procedure aa yielded the title compound as a light-yellow oil (0.44 g, 41 %). 1H-NMR (300 MHz, CDCl3-d) δ (ppm): 8.00 (d, J = 7.9 Hz, 1H), 7.66 (d, J = 7.3 Hz, 1H), 7.57 (d, J = 5.5 Hz, 1H), 7.51 - 7.37 (m, 2H).

19F NMR (282 MHz, CDCl3-d) δ (ppm): -63.13 (CF3).

Intermediate 3: 7-fluorobenzo[b]thiophene

General procedure aa yielded the title compound as a yellow oil (1.78 g, 52 %). 1H-NMR (300 MHz, CDCl3-d) δ (ppm) δ 7.63 (d, J = 8.0 Hz, 1H), 7.49 (d, J = 5.3 Hz, 1H), 7.41 - 7.30 (m, 2H), 7.07 (dd, J = 9.8, 8.0 Hz, 1H).

19F NMR (282 MHz, CDCl3-d) δ (ppm): -115.37 (CF).

Intermediate 4: 1-(Benzo[b]thiophen-7-yl)-2,2,2-trifluoroethan-1-one

This compound was synthetized using a modified procedure of J. Med. Chem. 2002, 45, 4038-4046. 7-bromobenzo[b]thiophene (1.0 g, 4.69 mmol, 1.0 eq.) was dissolved in diethyl ether (25 ml) and cooled at -40°C. Then n-butyllithium 1.6 M (3.23 ml, 5.16 mmol, 1.1 eq) was added dropwise and the solution was warmed to 0°C over a 1 h period. The solution was cooled to -60°C and a solution of 2,2,2-trifluoro-1-morpholinoethan-1-one (0.86 g, 4.69 mmol, 1.0 eq) in diethyl ether (5 ml) was added in portions. The resultant mixture was stirred at -60°C for 7 h and then warmed up to room temperature. The solution was hydrolyzed with saturated NH4Cl (5 ml), washed with NH4Cl (3 × 5 ml) and water (3 × 5 ml), dried (Na2SO4) and concentrated. The resulting residue was purified by flash column chromatography on silica gel using ethyl hexane: ethyl acetate (10:1) as eluent to afford a light-yellow solid (0.98 g, 91%).
1H-NMR (300 MHz, CDCl3) δ = 8.20 (ddd, J = 7.9, 4.5, 1.4 Hz, 2H), 7.70 (d, J = 5.5 Hz, 1H), 7.58 (t, J = 7.9 Hz, 1H), 7.49 (d, J = 5.5 Hz, 1H). 19F NMR (282 MHz, CDCl3) δ = - 69.54 (s, CF3).

Intermediate 5: Benzo[b]thiophene-5-carbonitrile

Nitrogen gas was bubbled through a mixture of 5-bromobenzo[b]thiophene (510 mg, 2.34 mmol) and zinc cyanide (299 mg, 2.49 mmol) in dry DMF (3 mL) for 5 minutes. Tetrakis(triphenylphosphine)palladium(0) (153 mg, 0.13 mmol) was added, the tube sealed and the mixture stirred at 90°C for 7h. The reaction mixture was filtered over Celite, and the filtrate was extracted using AcOEt and NaHCO3. The organic phase was concentrated and the residue purified by column chromatography (Yield: 82%).
1H-NMR (400 MHz, DMSO-d6) δ = 7.58 (dd, 1H), 7.72 (m, 1H), 7.99 (d, 1H), 8.26 (m, 1H), 8.43 (d, 1H).

Intermediate 6: Benzo[b]thiophene-7-carbonitrile

The title compound was synthesized according to the method described above for benzo[b]thiophene-5-carbonitrile.
1H-NMR (400 MHz, DMSO) δ= 7.59 (dd, 1H), 7.65 (d, 1H), 7.96 (dd, 1H), 8.01 (d, 1H), 8.26 (dd, 1H).

Intermediate 7: Benzo[b]thiophene-5-carboxylic acid

To a solution of benzo[b]thiophene-5-carbonitrile (400 mg, 2.51 mmol) in MeOH (40 mL), KOH (2.0 g, 35 mmol) and water 10 mL were added in portions, and the mixture was stirred at 80°C for 36 hours. After acidification with 2N HCI (pH 4-5), the solid was filtered off and washed with pentane to yield the title compound as an offwhite solid (390 mg, 87%).
1H-NMR (400 MHz, DMSO-d6) δ= 7.58 (dd, 1H), 7.72 (m, 1H), 8.01 (d, 1H), 8.29 (m, 1H), 8.46 (d, 1H), 12.11 (s, 1H).

Intermediate 8: Methyl benzo[b]thiophene-5-carboxylate

Benzo[b]thiophene-5-carboxylic acid (390 mg, 2.19 mmol) was refluxed in sat. HCI in MeOH (30 mL) for 24 hours. The methyl ester was obtained after evaporation of the solvent (340 mg, 76%).
1H-NMR (400 MHz, DMSO-d6) δ= 3.85 (s, 3H), 7.59 (dd, 1H), 7.73 (m, 1H), 7.98 (d, 1H), 8.27 (m, 1H), 8.44 (d, 1H).

Intermediate 9: 5-Fluorobenzo[b]thiophene-7-carbonitrile Synthesis of 7-Bromo-5-fluorobenzo[b]thiophene

General procedure aa afforded the title compound as a light-yellow oil (1.02 g) that was used as such in the next step.

Synthesis of 5-Fluorobenzo[b]thiophene-7-carbonitrile

A solution of 7-bromo-5-fluorobenzo[b]thiophene (1.02 g, 4.41 mmol, 1.0 eq.) and copper cyanide (0.590 g, 6.62 mmol, 1.5 eq.) in N-methyl-2-pirrolydone (12 ml) was stirred for 2 days in a sealed tube at 200 °C. The mixture was cooled to room temperature, poured into sat. aq. NaHCO3 (30 mL) and kept at 4 °C for 60 min. The solid was filtered off and dissolved in a mixture of 40 mL NH4OH(28%)/NH4Cl(sat) (1:1) and ethyl acetate (60 mL) and stirred for 1h. Then, it was washed with ethyl acetate (x3), dried (Na2SO4), concentrated and purified by flash column chromatography on silica gel using hexane/diethyl ether (2%) as eluent to afford 5-fluoro-3-formylbenzo[b]thiophene-7-carbonitrile as a white solid (0.23 g, 29%).
1H-NMR (300 MHz, CDCl3) δ (ppm): 7.73 (dd, J = 8.8, 2.3 Hz, 1H), 7.69 (d, J = 5.5 Hz, 1H), 7.47 (dd, J = 8.1, 2.2 Hz, 1H), 7.39 (d, J = 5.5 Hz, 1H).

Intermediate 10: 4-Fluorobenzo[b]thiophene-7-carbonitrile

The title compound was prepared in a 2-step synthesis as described above for Intermediate 9 and was afforded as a white solid (0.33 g).
1H-NMR (300 MHz, CDCl3) δ (ppm): 7.70 (dd, J = 8.2, 4.5 Hz, 1H), 7.61 (d, J = 5.5 Hz, 1H), 7.53 (d, J = 5.5 Hz, 1H), 7.13 (t, J = 8.8 Hz, 1H).

Intermediate 11: 5,7-Dichlorobenzo[b]thiophene

General procedure aa afforded the title compound as a light-yellow oil (1.57 g). 1H-NMR (300 MHz, CDCl3) δ (ppm): 7.78 (dd, J = 8.0, 0.8 Hz, 1H), 7.52 (s, 1H), 7.50 (s, 1H), 7.46 - 7.42 (m, 1H).

Intermediate 12: 3-Bromo-6-chlorobenzo[b]thiophene-7-carbonitrile

To a solution of 2-chloro-6-mercaptobenzonitrile (1.5 g, 8.84 mmol, 1.0 eq.) in ethanol (20 ml) was added a solution of sodium hydroxide (0.35 g, 8.84 mmol, 1.0 eq.) in water (7 ml). Then, a solution of 2-bromoacetic acid (1.35 g, 9.73 mmol, 1.1 eq.), sodium carbonate (0.51 g, 4.86 mmol, 0.5 eq.) in water (8 mL) was added, and the mixture was stirred for 2 hours. The pH of the mixture was adjusted (~ 2) with HCI (2N) and extracted with ethyl acetate (x2) and DCM (x2). The organic phase was dried (Na2SO4) and concentrated affording 2-((3-chloro-2-cyanophenyl) thio) acetic acid as a yellow oil. This product was used in the next step without further purification.
1H-NMR (300 MHz, CDCl3) δ (ppm): 7.51 - 7.44 (m, 2H), 7.42 - 7.35 (m, 1H), 3.79 (s, 2H), 2.83 (bs, 1H).

To a solution of 2-((3-chloro-2-cyanophenyl) thio) acetic acid (2.0 g, 8.78 mmol, 1.0 eq.) in chloroform (60 ml), thionyl chloride (0.96 mL, 13.18 mmol, 1.5 eq.) was dropwise added. The mixture was refluxed for 1 hour and then cooled to 0 °C, when aluminum chloride (10.54 g, 79.06 mmol, 9 eq.) was added in portions. The mixture was stirred at RT overnight, cooled to 0 °C before adding water, and then extracted with ethyl acetate (x3). The organic phase was dried (Na2SO4), concentrated and purified by flash column chromatography on silica gel using hexane/ethyl acetate (4:1) as eluent to afford 6-chloro-3-oxo-2,3-dihydrobenzo[b]thiophene-7-carbonitrile (1.53 g, 83%).
1H-NMR (300 MHz, CDCl3) δ (ppm): 7.48 (d, J = 8.5 Hz, 1H), 7.35 (d, J = 8.5 Hz, 1H), 3.95 (s, 2H).

A solution of 6-chloro-3-oxo-2,3-dihydrobenzo[b]thiophene-7-carbonitrile (1.53 g, 7.79 mmol, 1.0 eq.) and sodium borohydride (0.32 g, 8.57 mmol, 1.1 eq.) in ethanol (60 ml) was stirred for 1 hour at RT, concentrated, redissolved in ethyl acetate and washed with water. The organic phase was dried over Na2SO4 and concentrated to afford 6-chloro-3-hydroxy-2,3-dihydrobenzo[b]thiophene-7-carbonitrile which was used in the next step without further purification.

A solution of 6-chloro-3-hydroxy-2,3-dihydrobenzo[b]thiophene-7-carbonitrile (7.79 mmol, 1.0 eq.) and p-toluensulfonic acid (0.15 g, 78 mmol, 0.1 eq.) in toluene (20 ml) was refluxed for 1 hour, concentrated and purified by flash column chromatography on silica gel using hexane/ethyl acetate (6:1) as eluent to afford 6-chlorobenzo[b]thiophene-7-carbonitrile (1.26 g, 83%).
1H-NMR (300 MHz, CDCl3) δ (ppm): 7.93 (d, J = 8.6 Hz, 1H), 7.60 (d, J = 5.4 Hz, 1H), 7.47 (d, J = 8.6 Hz, 1H), 7.39 (d, J = 5.5 Hz, 1H).

A solution of 6-chlorobenzo[b]thiophene-7-carbonitrile (1.26 g, 6.50 mmol, 1.0 eq.), N-Bromo succinimide (1.74 g, 9.76 mmol, 1.5 eq.), acetic acid (0.04 mL, 0.64 mmol, 0.1 eq.), in N, N-dimethylformamide (10 ml) was heated at 130 °C for 1h in a microwave reactor. Then water was added, and the mixture was extracted with ethyl acetate (x3). The organic phase was dried (Na2SO4), concentrated and purified by flash column chromatography on silica gel using hexane/ethyl acetate (8:1) as eluent to afford 3-bromo-6-chlorobenzo[b]thiophene-7-carbonitrile (1.33 g, 75%).

Intermediate 13: 3-Bromo-5-fluorothieno[2,3-b]pyridine

To a solution of 5-fluoropyridin-2(1H)-one (1.0 g, 8.84 mmol, 1.0 eq.) in dry toluene (20 ml) was added N-iodosuccinimide (1.99 g, 8.84 mmol, 1.0 eq). The resultant mixture was stirred for 30 minutes at 90 °C, then cooled to RT and concentrated. The residue was purified by flash column chromatography on silica gel using hexane/ethyl acetate (2:1) as eluent to afford 5-fluoro-3-iodopyridin-2-ol as a yellow solid (1.4 g, 65%).
1H-NMR (300 MHz, DMSO-d6) δ (ppm): 11.81 (s, 1H), 10.99 (bs, 1H), 8.18 (dd, J= 7.2, 3.1 Hz, 1H), 7.65 (t, J = 3.1 Hz, 1H).

A solution of 5-fluoro-3-iodopyridin-2-ol (0.5 g, 2.06 mmol, 1 eq.), palladium acetate (0.005 g, 0.02 mmol, 0.01 eq.), triphenylphosphine (0.011 g, 0.041 mmol, 0.02 eq.), copper iodide (0.008 g, 0.041 mmol, 0.02 eq.) in dry tetrahydrofuran (15 ml) was purged with argon gas for 5 minutes. Then, trimethylsilylacetylene (0.43 mL, 3.10 mmol, 1.5 eq) and propylamine (0.34 mL, 4.13 mmol, 2 eq) was added, and the mixture was stirred at 38 °C for 60 minutes, concentrated, taken up in ethyl acetate and washed with Rochelle salt (x2), hydrochloride acid 0.1 N (x2) and saturated sodium bicarbonate (x2). The organic phase was dried (Na2SO4), filtered, concentrated and purified by flash column chromatography on silica gel using hexane/ ethyl acetate (2:1) as eluent to afford 5-fluoro-3-((trimethylsilyl)ethynyl)pyridin-2-ol as a light yellow oil (0.263 g, 61%).
1H-NMR (300 MHz, DMSO-d6) δ (ppm): 13.57 (bs, 1H), 7.60 (dd, J = 7.6, 3.1 Hz, 1H), 7.39 (t, J = 3.1 Hz, 1H), 0.26 (s, 9H).

To a solution of 5-fluoro-3-((trimethylsilyl)ethynyl)pyridin-2-ol (0.26 g, 1.25 mmol, 1 eq.) in dry toluene (10 ml) was added Lawesson's reagent (0.0.25 mL, 0.63 mmol, 0.5 eq). The mixture was stirred at 120 °C for 60 minutes, concentrated and purified by flash column chromatography on silica gel using ethyl hexane: ethyl acetate (7:1) as eluent to afford 5-fluoro-2-(trimethylsilyl)thieno[2,3-b]pyridine as a yellow solid (0.22 g, 76%).
1H-NMR (300 MHz, DMSO-d6) δ (ppm): 3.68 (d, J = 2.4 Hz, 1H), 2.95 (dd, J = 9.0, 2.4 Hz, 1H), 2.58 (s, 1H), -4.37 (s, 9H).

To a solution of 5-fluoro-2-(trimethylsilyl)thieno[2,3-b]pyridine 0.22 g, 0.96 mmol, 1 eq.) in ethanol (4 ml) was added potassium carbonate (0.332 g, 2.40 mmol, 2.5 eq.). The mixture was stirred at 65 °C for 60 minutes. Then, the solid was filtered off and washed with dichloromethane. The filtrate was concentrated, taken up in ethyl acetate, washed with water (×2) and brine (×2), dried (Na2SO4) and concentrated, and the residue was purified by flash column chromatography on silica gel using hexane/ ethyl acetate (20:1) as eluent to afford 5-fluorothieno[2,3-b]pyridine as a light yellow oil (0.111 g, 75%).
1H-NMR (300 MHz, CDCL) δ (ppm): 8.47 (d, J = 2.4 Hz, 1H), 7.75 (dd, J = 8.9, 2.7 Hz, 1H), 7.63 (d, J = 6.0 Hz, 1H), 7.24 (d, J = 6.0 Hz, 1H).

To a solution of 5-fluorothieno[2,3-b]pyridine 0.78 g, 5.12 mmol, 1 eq.) in water (8mL) was added hydrobromic acid (5.22 mL, 46.09 mmol, 9 eq.) and bromine (0.39 g, 7.68 mmol, 1.5 eq.) and this mixture was stirred at 55 °C for 20 hours. The solid was filtered off and washed with saturated sodium bicarbonate, then taken up in ethyl acetate, washed with saturated sodium bicarbonate (×2), dried (Na SO4), concentrated and purified by flash column chromatography on silica gel using hexane/ ethyl acetate (20:1) as eluent to afford 3-bromo-5-fluorothieno[2,3-b]pyridine as a light yellow oil (0.73 g, 62%).
1H-NMR (300 MHz, CDCL) δ (ppm): 8.51 (s, 1H), 7.76 (dd, J = 8.5, 2.5 Hz, 1H), 7.64 (s, 1H).

General procedure a Intermediate a1: N-Cyclopropylbenzo[b]thiophene-5-carboxamide

To a solution of benzo[b]thiophene-5-carboxylic acid (700 mg, 3.93 mmol), EDC*HCl (1130 mg, 5.89 mmol), HOBT (601 mg, 3.93 mmol) in 15 mL of DMF at 5°C, were added cyclopropyl amine (336 mg, 5.89 mmol) and DIPEA (1270 g, 9.89 mmol). The reaction mixture was stirred for 18 hours at room temperature. The solution was poured into NaHCO saturated and the precipitate was washed with three times of cold water and one time of pentane to obtain the desired amide derivative (700 mg, 82%).
1H-NMR (400 MHz, DMSO-d6) δ= 0.60 (m, 2H), 0.71 (m, 2H), 2.93 (m, 1H), 7.56 (dd, 1H), 7.67 (d, 1H), 7.93 (d, 1H), 8.22 (dd, 1H), 8.46 (d, 1H).

The following derivatives were synthesized according to General procedure a as described above and were used in the next step without further purification.

Intermediate a2: N-(Cyclopropylmethyl)benzo[b] thiophene-5-carboxamide

1H-NMR (400 MHz, DMSO-d6) δ= 0.20 (m, 2H), 0.47 (m, 2H), 1.05 (m, 1H), 3.14 (m, 2H), 7.49 (dd, 1H), 7.63 (d, 1H), 7.89 (d, 1H), 8.18 (dd, 1H), 8.30 (t, 1H), 8.42 (d, 1H).

Intermediate a3: Benzo[b]thiophen-5-yl(4-methylpiperazin-1-yl)methanone

1H-NMR (400 MHz, DMSO-d6) δ= 2.15 (s, 3H), 2.29 (m, 4H), 3.41 (m, 4H), 7.47 (dd, 1H), 7.61 (d, 1H), 7.97 (d, 1H), 8.15 (dd, 1H), 8.50 (d, 1H).

Intermediate a4: Benzo[b]thiophen-5-yl(morpholino)methanone

1H-NMR (400 MHz, DMSO-d6) δ= 3.49 (m, 4H), 3.65 (m, 4H), 7.47 (dd, 1H), 7.61 (d, 1H), 7.97 (d, 1H), 8.15 (dd, 1H), 8.50 (d, 1H).

Intermediate a5: N-Cyclopropylbenzo[b]thiophene-7-carboxamide

1H-NMR (400 MHz, DMSO) δ= 0.62 (m, 2H), 0.74 (m, 2H), 2.91 (m, 1H), 7.47 (dd, 2H), 7.81 (d,1H), 7.92 (d, 1H), 8.04 (dd, 1H), 8.69 (d, 1H).

Intermediate a6: Benzo[b]thiophen-7-yl(4-methylpiperazin-1-yl)methanone

1H-NMR (400 MHz, DMSO) δ= 2.19 (s, 3H), 2.28 (d, 4H), 3.42 (t, 4H), 7.37 (d, 1H), 7.46 (dd, 1H), 7.51 (d, 1H), 7.83 (d, 1H), 7.96 (dd, 1H).

General procedure b Intermediate b1: 4-(Benzo[b]thiophen-5-yl)morpholine

A solution of 5-bromobenzo[b]thiophene (600 mg, 2.82 mmol), morpholine (368 mg, 4.23 mmol), Pd2(dba)3 (129 mg, 0.14 mmol), tricyclohexylphosphine (119 mg, 0.42 mmol) and NatButO (541 mg, 8.46 mmol) in dry toluene (20 mL) under N2 atmosphere was refluxed for 24 hours. After cooling, the muxture was poured into sat. aq. NaHCO3, extracted with AcOEt (3X), dried over Na2SO4, filtered over and concentrated. The product was isolated as grey solid after flash column chromatography (230 mg, 37%).
1H-NMR (400 MHz, DMSO-d6) δ= 3.45 (m, 4H), 3.23 (m,4H), 6.94 (d, 1H), 7.42 (d, 1H), 7.54 (dd, 1H), 7.66 (d, 1H), 7.71 (d, 1H).

The following derivatives were synthesized according to General procedure b as described above and were used in the next step without further purification:

Intermediate b2: 1-(Benzo[b]thiophen-5-yl)-4-methylpiperazine

1H-NMR (400 MHz, DMSO-d6) δ= 2.25 (s, 3H), 2.53 (m, 4H), 3.16 (m,4H), 6.93 (d, 1H), 7.39 (d, 1H), 7.61 (d, 1H), 7.51 (dd, 1H), 7.68 (d, 1H).

Intermediate b3: N-Benzylbenzo[b]thiophen-5-amine

1H-NMR (400 MHz, DMSO-d6) δ= 5.06 (d, 2H), 6.91 (d, 1H), 7.17 (m, 1H), 7.24 (m, 2H), 7.27 (m, 2H) 7.32 (d, 1H), 7.49 (d, 1H), 7.51 (dd, 1H), 7.74 (t, 1H), 7.81 (d, 1H).

Intermediate b4: 1-(Benzo[b]thiophen-7-yl)-4-methylpiperazine

1H-NMR (400 MHz, DMSO-d6) δ= 2.27 (s, 3H), 2.54 (m, 4H), 3.14 (m, 4H), 6.97 (d, 1H),7.33 (t, 1H),7.43 (d, 1H), 7.55 (d, 1H), 7.71 (d, 1H).

PREPARATION OF 3-FORMYLBENZO[b]THIOPHENE INTERMEDIATES General procedure c Intermediate c1: 3-Formylbenzo[b]thiophene-5-carbonitrile

5-cyanebenzo[b]thiophene (320 mg, 2 mmol) was dissolved in dry dichloroethane (4 mL) under a nitrogen atmosphere. Dichloro(methoxy)methane (0.277 mL, 3 mmol) and titanium tetrachloride (0.33 mL) were added dropwise. The mixture was stirred at RT for 20h, then poured onto a mixture of NaHCO3 solution and ice and extracted with DCM. The organic phases were combined and concentrated, and the residue was filtered off and rinsed with diethyl ether. (Yield: 32 %).
1H-NMR (400 MHz, DMSO-d6) δ= 7.89 (dd, 1H), 8.39 (m, 1H), 8.86 (m, 1H), 9.16 (s, 1H), 10.16 (d, 1H).

The following derivatives were synthesized according to General procedure c as described above and were used in the next step without further purification:

Intermediate c2: 5-Fluorobenzo[b]thiophene-3-carbaldehyde

535 mg, 90%.

1H-NMR (400 MHz, DMSO-d6) δ= 7.42 (m, 1H) 8.21 (m, 2H), 9.09 (s, 1H), 10.11 (s, 1H).

Intermediate c3: 5-Morpholinobenzo[b]thiophene-3-carbaldehyde

1H-NMR (400 MHz, DMSO-d6) δ= 3.46 (m, 4H), 3.23 (m,4H), 6.95 (d, 1H), 7.55 (dd, 1H), 7.73 (d, 1H), 9.05 (s, 1H), 10.16 (s, 1H).

Intermediate c4: 5-(4-Methylpiperazin-1-yl)benzo[b]thiophene-3-carbaldehyde

1H-NMR (400 MHz, DMSO-d6) δ= 2.19 (s, 3H), 2.49 (m, 4H), 3.25 (m,4H), 7.23 (d, 1H), 7.89 (d, 1H), 8.05 (d, 1H), 8.31 (s, 1H), 10.11 (s, 1H).

Intermediate c5: 5-(Benzylamino)benzo[b]thiophene-3-carbaldehyde

1H-NMR (400 MHz, DMSO-d6) δ= 4.98 (d, 2H), 6.98 (d, 1H), 7.23 (dd, 1H), 7.17 (m, 1H), 7.24 (m, 2H), 7.27 (m, 2H), 7.99 (d, 1H),8.24 (t, 1H) 8.51 (s, 1H), 10.12 (s, 1H).

Intermediate c6: N-Cyclopropyl-3-formylbenzo[b]thiophene-5-carboxamide

1H-NMR (400 MHz, DMSO-d6) δ= 0.60 (m, 2H), 0.71 (m, 2H), 2.93 (m, 1H), 7.68 (t, 1H), 7.97 (d, 1H), 8.16 (d, 1H), 8.56 (dd, 1H), 9.09 (s, 1H), 10.15 (s, 1H).

Intermediate c7: N-(Cyclopropylmethyl)-3-formylbenzo[b]thiophene-5-carboxamide

1H-NMR (400 MHz, DMSO-d6) δ= 0.27 (m, 2H), 0.52 (m, 2H), 2.26 (m, 1H), 3.27 (m, 2H), 7.95 (d, 1H), 8.18 (dd, 1H), 8.48 (t, 1H), 8.53 (d, 1H) 9.04 (s, 1H), 10.12 (s, 1H).

Intermediate c8: 5-(4-Methylpiperazine-1-carbonyl)benzo[b]thiophene-3-carbaldehyde

1H-NMR (400 MHz, DMSO-d6) δ= 2.16 (s, 3H), 2.29 (m, 4H), 3.41 (m, 4H), 7.97 (d, 1H), 8.17 (d, 1H), 8.50 (d, 1H), 9.06 (s, 1H), 10.11 (s, 1H).

Intermediate c9: 5-(morpholine-4-carbonyl)benzo[b]thiophene-3-carbaldehyde

1H-NMR (400 MHz, DMSO-d6) δ= 3.51 (m, 4H), 3.66 (m, 4H), 7.96 (d, 1H), 8.18 (d, 1H), 8.51 (d, 1H), 9.08 (s, 1H), 10.13 (s, 1H).

Intermediate c10: 3-Formylbenzo[b]thiophene-7-carbonitrile

1H-NMR (400 MHz, DMSO-d6) δ= 7.70 (t, 1H), 8.16 (d, 1H), 8.81 (d, 1H), 9.08 (s, 1H), 10.16 (s, 1H).

Intermediate c11: 7-(4-Methylpiperazin-1-yl)benzo[b]thiophene-3-carbaldehyde

1H-NMR (400 MHz, DMSO-d6) δ= 2.91 (s, 4H), 3.30 (s, 6H), 3.49 (s, 9H),7.16 (d, 1H), 7.35 (t, 1H),7.97 (d, 1H), 8.04 (d, 1H), 8.30 (d, 1H),10.10 (s, 1H).

Intermediate c12: 7-(4-methylpiperazine-1-carbonyl)benzo[b]thiophene-3-carbaldehyde

1H-NMR (400 MHz, DMSO-d6) δ= 3.41 (dd, 4H), 3.49 (dd, 4H), 3.90 (s, 3H), 7.66 (m, 1H), 8.23 (s, 2H), 9.08 (s, 1H), 10.15 (s, 1H).

Intermediate c13: N-Cyclopropyl-3-formylbenzo[b]thiophene-7-carboxamide

1H-NMR (400 MHz, DMSO-d6) δ= 0.64 (dt, 2H), 0.75 (td, 2H), 2.93 (m, 1H), 7.64 (t, 1H), 8.08 (dd, 1H), 8.72 (dd, 1H), 8.84 (d, 1H), 9.04 (s, 1H), 10.14 (s, 1H).

Intermediate c14: Methyl 3-formylbenzo[b]thiophene-5-carboxylate

The title compound was synthesized according to General procedure c and purified by trituration with n-pentane to yield a pale brown solid (320 mg, 83%). 1H-NMR (400 MHz, DMSO-d6) δ= 3.97 (s, 3H), 7.73 (m, 1H), 8.20 (m, 1H), 8.85 (dd, 1H), 9.08 (s, 1H), 10.15 (s, 1H).

The ester was hydrolysed as follows:

Intermediate c15: 3-Formylbenzo[b]thiophene-5-carboxylic acid

To a solution of methyl 3-formylbenzo[b]thiophene-5-carboxylate (320 mg, 1.45 mmol) in EtOH (15 mL) and water (5 mL), litium hydroxide (230 mg, 9.6 mmol) was added at 0°C. The mixture was stirred for 24 hours at room temperature. The product was isolated as a pale yellow solid after acidification (pH 4-5) with 2N HCl, followed by filtration and pentane washing (260 mg, 92% yield).
1H-NMR (400 MHz, DMSO-d6) δ= 7.81 (m, 1H), 8.23 (m, 1H), 8.88 (dd, 1H), 9.08 (s, 1H), 10.15 (s, 1H), 12.45 (s, 1H).

Intermediate c16: 7-(2,2,2-Trifluoroacetyl)benzo[b]thiophene-3-carbaldehyde

The title compound was synthesized according to General procedure c and purified by flash column chromatography yielding a yellow solid (0.31 g, 91%). 1H-NMR (300 MHz, CDCl3) δ= 10.22 (s, 1H), 9.20 (s, 1H), 9.03 (d, J = 8.0 Hz, 1H), 8.34 (d, J = 7.0 Hz, 1H), 7.88 (t, J = 7.9 Hz, 1H). 19F NMR (282 MHz, CDCl3) δ = - 68.92 (s, CF3).

Intermediate c17: 7-Chlorobenzo[b]thiophene-3-carbaldehyde

The title compound was synthesized according to General procedure c and purified by flash column chromatography yielding a yellow solid (0.38 g, 48 %). 1H-NMR (300 MHz, CDCl3-d) δ (ppm) δ 10.13 (s, 1H), 8.66 - 8.51 (m, 1H), 8.35 (s, 1H), 7.53 - 7.41 (m, 2H).

Intermediate c18: 7-(Trifluoromethyl)benzo[b]thiophene-3-carbaldehyde

The title compound was synthesized according to General procedure c and purified by flash column chromatography yielding a yellow solid (0.081 g, 16 %).
1H-NMR (300 MHz, CDCl3-d) δ (ppm): 10.18 (s, 1H), 8.92 (d, J = 8.0 Hz, 1H), 8.43 (s, 1H), 7.78 (d, J = 7.5 Hz, 1H), 7.63 (t, J = 8.0 Hz, 1H).
19F NMR (282 MHz, CDCl3-d) δ (ppm): -62.56 (CF3).

Intermediate c19: 7-Fluorobenzo[b]thiophene-3-carbaldehyde

The title compound was synthesized according to General procedure c and purified by flash column chromatography yielding a yellow solid (0.27 g, 19 %). 1H-NMR (300 MHz, CDCl3-d) δ (ppm): 10.15 (s, 1H), 8.45 (d, J = 8.1 Hz, 1H), 8.34 (s, 1H), 7.48 (td, J = 8.1, 5.1 Hz, 1H), 7.22 - 7.09 (m, 1H).
19F NMR (282 MHz, CDCl3-d) δ (ppm): -115.33 (CF).

Intermediate c20: Thieno[2,3-b]pyridine-3-carbaldehyde

Step 1: 3-Bromo-thieno[2,3-b]pyridine (500 mg, 2.35 mmol) and copper (I) cyanide (315 mg, 3.53 mmol) were suspended in NMP (10 mL) and stirred at 200°C for 20 hours under N2 atmosphere. The solution was poured into sat. aq. NaHCO3, filtered and washed with water to afford thieno[2,3-b]pyridine-3-carbonitrile as a grey solid (376 mg, 95% yield).
1H-NMR (400 MHz, DMSO-d6) δ= 7.65 (m, 1H), 8.39 (dd, 1H), 8.75 (m, 1H), 9.04 (d, 1H).

Step 2: To a solution of diisobutylaluminum hydride (DIBAL) (25%w in toluene, 2.7 mL, 4.1 mmol) in toluene (45 mL) at -78°C under N2 atmosphere, a solution of thieno[2,3-b]pyridine-3-carbonitrile (660 mg, 4.1 mmol) in toluene (4 mL) was added dropwise. The temperature was allowed to rise to 0°C and the mixture was stirred until completion. Then, water (30 mL) was added slowly and the product was extracted with AcOEt (3X), dried over Na2SO4, filtered over silica gel and concentrated. The title compound was obtained as a brown solid and used in the next step without further purification (569 mg, 85% yield).
1H-NMR (400 MHz, DMSO-d6) δ= 7.60 (m, 1H), 8.68 (t, 1H), 8.80 (d, 1H), 9.08 (d, 1H), 10.06 (s, 1H).

Intermediate c21: 5-Fluoro-3-formylbenzo[b]thiophene-7-carbonitrile

General procedure c gave the title compound as a yellow solid (0.153 g, 58 %).
1H-NMR (300 MHz, CDCl3-d) δ (ppm): 10.14 (s, 1H), 8.68 (d, J = 9.1 Hz, 1H), 8.53 (s, 1H), 7.59 (d, J = 9.1 Hz, 1H).

Intermediate c22: 4-Fluoro-3-formylbenzo[b]thiophene-7-carbonitrile

General procedure c gave the title compound as a yellow solid (0.125 g, 33 %).
1H-NMR (300 MHz, CDCl3-d) δ (ppm): 10.40 (s, 1H), 8.57 (s, 1H), 7.82 (dd, J = 8.1, 4.4 Hz, 1H), 7.40 - 7.28 (m, 1H).

Intermediate c23: 3-Formylthieno[3,2-b]pyridine-7-carbonitrile

This aldehyde was prepared in 4 steps using a different method.

First step: A solution of 7-chlorothieno[3,2-b]pyridine (3.0 g, 17.68 mmol, 1.0 eq.), hydrobromic acid (30 mL, 265.28 mmol, 15 eq.) and bromine (2.28 mL, 44.20 mmol, 2.5 eq.) in water (40 ml) was stirred at 80°C for 2 days. The mixture was cooled to room temperature and NaHCO3 was added until pH 8. The mixture was washed with ethyl acetate (x3) and dichloromethane (x2), dried over Na2SO4, concentrated and purified by flash column chromatography on silica gel using hexane/ethyl acetate (15:1) as eluent to afford 3-bromo-7-chlorothieno[3,2-b]pyridine as a yellow solid (3.45 g, 79%).
1H-NMR (300 MHz, CDCl3-d) 5 (ppm): 8.74 (dd, J = 5.0, 0.4 Hz, 1H), 7.84 (s, 1H), 7.44 - 7.30 (m, 1H).

Second step: To a solution of 3-bromo-7-chlorothieno[3,2-b]pyridine (1.2 g, 4.82 mmol, 1.0 eq.), bis(triphenylphosphine)palladium (II) dichloride (0.338 g, 0.48 mmol, 0.1 eq.) and potassium carbonate (2.0 g, 14.48 mmol, 3 eq.) in a mixture of dimethylether and water (3:1) (32 mL) was added (E)-styrylboronic acid (0.92 g, 6.28 mmol, 1.3 eq). The resultant mixture was stirred at 110 °C for 1 hour. The mixture was cooled to RT, filtered over celite, washed with DCM, concentrated and purified by flash column chromatography on silica gel using hexane/ethyl acetate (10:1) as eluent to afford (E)-7-chloro-3-styrylthieno[3,2-b]pyridine as a yellow oil ( 0.870 g, 66%).
1H-NMR (300 MHz, CDCl3-d) δ (ppm): 8.69 (d, J = 5.6 Hz, 1H), 7.83 (s, 1H), 7.76 (d, J = 16.4 Hz, 1H), 7.61 (d, J = 8.2 Hz, 2H), 7.53 (d, J = 16.4 Hz, 1H), 7.42 - 7.33 (m, 3H), 7.29 (d, J = 7.1 Hz, 1H).

Third step: A solution of (E)-7-chloro-3-styrylthieno[3,2-b]pyridine (0.870 g, 3.20 mmol, 1.0 eq.), zinc cyanide (0.75 g, 6.40 mmol, 2 eq.), tris(benzylideneacetone)dipalladium (0) (0.092 g, 0.16 mmol, 0.05 eq.), 1,1'ferrocenediyl-bis-(diphenylphosphine) (0.234 g, 0.32 mmol, 0.1 eq.) and zinc (0.063 mL, 0.96 mmol, 0.3 eq.) in N,N-dimethylformamide (7 ml) was stirred at 160°C for 90 min. The mixture was cooled to RT, filtered over celite, washed with DCM, concentrated and purified by flash column chromatography on silica gel using hexane/ethyl acetate (7:1) as eluent to afford (E)-3-styrylthieno[3,2-b]pyridine-7-carbonitrile as a light yellow solid (0.38 g, 47%).

Fourth step: A mixture of (E)-3-styrylthieno[3,2-b]pyridine-7-carbonitrile (0.370 g, 1.47 mmol, 1 eq.) and DCM (10 mL) at -78 °C was purged with N2 for 5 min. Then, ozone was bubbled into the mixture for 15 min and dimethyl sulphide (1.1 mL, 14.78 mmol, 10 eq.) was added. The mixture was stirred at -78 °C with a nitrogen current for 30 min, concentrated and purified by flash column chromatography on silica gel using hexane/ethyl acetate (7:1) as eluent to afford 3-formylthieno[3,2-b]pyridine-7-carbonitrile as a light yellow solid (0.125 g, 45%).
1H-NMR (300 MHz, CDCl3-d) δ (ppm): 10.53 (s, 1H), 9.01 (d, J = 4.7 Hz, 1H), 8.78 (s, 1H), 7.67 (d, J = 4.7 Hz, 1H).

Intermediate c24: 3-Formylbenzo[b]thiophene-5,7-dicarbonitrile

This aldehyde was prepared starting from 5,7-dichlorobenzo[b]thiophene following the 4-step synthesis described for Intermediate c23, except that the cyanation reaction was done before the bromination step.

Yellow solid (0.22 g).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 10.18 (s, 1H), 9.28 (d, J = 1.2 Hz, 1H), 8.60 (s, 1H), 8.04 (d, J = 1.2 Hz, 1H).

Intermediate c25: 6-Chloro-3-formylbenzo[b]thiophene-7-carbonitrile

This aldehyde was prepared starting from 3-bromo-6-chlorobenzo[b]thiophene-7-carbonitrile following Steps 2 and 4 of the synthesis described for Intermediate c23. Yellow solid (0.29 g).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 10.13 (s, 1H), 8.84 (d, J = 8.8 Hz, 1H), 8.44 (s, 1H), 7.63 (d, J = 8.8 Hz, 1H).

Intermediate c26: 5-Fluorothieno[2,3-b]pyridine-3-carbaldehyde

A solution of 3-bromo-5-fluorothieno[2,3-b]pyridine (0.73 g, 3.14 mmol, 1.0 eq.) and copper cyanide (0.42 g, 4.72 mmol, 1.5 eq.) in N-methyl-2-pirrolydone (18 ml) was stirred for 2 days in a sealed tube at 200 °C, then cooled to RT and poured into saturated aqueous NaHCO3 (30 mL). This mixture was stirred at 4 °C for 60 min. The solid was filtered off and dissolved in a mixture of 40 mL of NH4OH (28%)/NH4Cl(sat) (1:1) and ethyl acetate (60 mL) which was stirred for 1h and then extracted with ethyl acetate (x3). The organic phase was dried over Na2SO4, filtered, concentrated and purified by flash column chromatography on silica gel using hexane/ethyl acetate (20:1) as eluent to afford 5-fluorothieno[2,3-b]pyridine-3-carbonitrile as a white solid (0.32 g, 56%).
1H-NMR (300 MHz, CDCl3-d) δ (ppm): 8.61 (d, J = 2.4 Hz, 1H), 8.32 (s, 1H), 7.96 (dd, J = 8.1, 2.7 Hz, 1H).

To a solution of 5-fluorothieno[2,3-b]pyridine-3-carbonitrile (0.32 g, 1.77 mmol, 1 eq.) in toluene (30 ml) at -78 °C was dropwise added a solution of DIBAI-H (1.98 mL, 1.94 mmol, 1.1 eq) in toluene. This mixture was stirred at -78 °C for 5 minutes, then at - 40 °C for 6 hours, and finally it was allowed to warm up to RT. After the addition of water the mixture was extracted with ethyl acetate (x2) which was dried (Na2SO4), concentrated and the residue purified by flash column chromatography on silica gel using hexane/ ethyl acetate (10:1) as eluent to afford 5-fluorothieno[2,3-b]pyridine-3-carbaldehyde as a light yellow oil (0.149 g, 47%).
1H-NMR (300 MHz, CDCl3-d) δ (ppm): 10.05 (s, 1H), 8.63 (dd, J = 8.8, 2.6 Hz, 1H), 8.56 (d, J = 2.6 Hz, 1H), 8.50 (s, 1H).

PREPARATION OF DICARBONYL INTERMEDIATES

Dicarbonyl compounds were synthesized according to the literature as described in the general procedures below.

General procedure d(adapted from Haibin Mao et al., Chem. Int. Ed. 2013, 52, 6288-6291)

To a solution of 2,2,6-trimethyl-4H-1,3-dioxin-4-one (1 eq) in toluene (5 M) at room temperature, the corresponding alcohol or amine (1 eq) was added. The mixture was heated at 150 °C for 6 hours, then allowed to cool to room temperature and concentrated in vacuum. The remaining residue was purified by flash column chromatography on silica gel using ethyl acetate/hexane as eluent to afford the dicarbonyl compound.

The following dicarbonyl compounds were synthesized following General procedure d.

Intermediate d1: 2,2,2-Trifluoroethyl 3-oxobutanoate

Light brown oil (8.93 g, 70 %).

1H-NMR (300 MHz, CDCl3) δ = 4.53 (q, J = 8.4 Hz, 1H), 3.57 (s, 1H), 2.28 (s, 1H). 19F NMR (282 MHz, CDCl3) δ = -73.92.

Intermediate d2: N,N-Diethyl-3-oxobutanamide

Yellow oil (1.5 g, 91%).

1H-NMR (300 MHz, CDCl3) δ = 3.49 (s, 2H), 3.39 (q, J = 7.1 Hz, 2H), 3.28 (q, J = 7.2 Hz, 2H), 2.28 (s, 3H), 1.15 (dt, J = 10.6, 7.1 Hz, 6H).

Intermediate d3: 1-(4-Methylpiperazin-1-yl)butane-1,3-dione

Yellow oil (1.8 g, 91 %).

1H-NMR (300 MHz, CDCl3) δ = 3.69 - 3.61 (m, 2H), 3.55 (s, 2H), 3.47 - 3.38 (m, 2H), 2.44 - 2.33 (m, 4H), 2.30 (s, 3H), 2.27 (s, 3H).

Intermediate d4: 1-Morpholinobutane-1,3-dione

Dark yellow oil (1.6 g, 88 %).

1H-NMR (300 MHz, CDCl3) δ = 3.72 - 3.58 (m, 6H), 3.56 (s, 2H), 3.50 - 3.35 (m, 2H), 2.28 (s, 3H).

Intermediate d5: 2-Methoxyethyl 3-oxobutanoate

Yellow oil (1.5 g, 91 %).

1H-NMR (300 MHz, CDCl3) δ = 4.34 - 4.24 (m, 2H), 3.65 - 3.55 (m, 2H), 3.49 (s, 2H), 3.38 (s, 3H), 2.27 (s, 3H).

Intermediate d6: 3-Acetamidopropyl 3-oxobutanoate

General procedure d using N-(3-hydroxypropyl)acetamide (prepared according to K. Veejendra et al., J. Org. Chem. 2004, 69, 577-580) yielded light yellow oil (2.1 g, 92 %).
1H-NMR (300 MHz, CDCl3) δ = 5.96 (bs, 1H), 4.22 (t, J = 6.0 Hz, 2H), 3.50 (s, 2H), 3.33 (q, J = 6.3 Hz, 2H), 2.28 (s, 3H), 1.98 (s, 3H), 1.93 - 1.80 (m, 2H).

Intermediate d7: Benzyl 3-oxobutanoate

Light yellow oil (2 g, 98 %).

1H-NMR (300 MHz, CDCl3) δ = 7.36 (s, 5H), 5.18 (s, 2H), 3.50 (s, 2H), 2.25 (s, 3H).

Intermediate d8: 2-Morpholinoethyl 3-oxobutanoate

Light yellow oil (2 g, 98 %).

1H-NMR (300 MHz, CDCl3) δ = 4.27 (t, J = 5.8 Hz, 2H), 3.73 - 3.64 (m, 4H), 3.47 (s, 2H), 2.63 (t, J = 5.8 Hz, 2H), 2.51 - 2.44 (m, 4H), 2.28 (s, 3H).

Intermediate d9: 2-(Dimethylamino)ethyl 3-oxobutanoate

Light yellow oil (2.1 g, 92 %).

1H-NMR (300 MHz, CDCl3) δ = 4.24 (t, J = 5.7 Hz, 2H), 3.48 (s, 2H), 2.57 (t, J= 5.7 Hz, 2H), 2.27 (s, 9H).

Intermediate d10: 2-Acetamidoethyl 3-oxobutanoate

Yellow oil (1.1 g, 91%).

1H-NMR (300 MHz, CDCl3) δ = 4.26 (t, J = 4.6 Hz, 2H), 3.56 - 3.51 (m, 4H), 2.28 (s, 3H), 2.00 (s, 3H).

Intermediate d11: Cyclohexylmethyl 3-oxobutanoate

Light yellow oil (1.4 g, 67%).

1H-NMR (300 MHz, CDCl3) δ = 3.95 (d, J = 6.4 Hz, 2H), 3.44 (s, 2H), 2.27 (s, 3H), 1.78 - 1.56 (m, 6H), 1.33 - 1.09 (m, 3H), 1.06 - 0.83 (m, 2H).

Intermediate d12: Pyridin-4-ylmethyl 3-oxobutanoate

Yellow oil (1.2 g, 89%).

1H-NMR (300 MHz, CDCl3) δ = 8.61 (d, J = 5.6 Hz, 2H), 7.25 (d, J = 4.1 Hz, 2H), 5.19 (s, 2H), 3.57 (s, 2H), 2.28 (s, 3H).

Intermediate d13: Pyridin-2-ylmethyl 3-oxobutanoate

Yellow oil (1.0 g, 74%).

1H-NMR (300 MHz, CDCl3) δ = 8.58 (ddd, J = 4.9, 1.8, 1.0 Hz, 1H), 7.71 (td, J = 7.7, 1.8 Hz, 1H), 7.38 (ddt, J = 7.8, 1.1, 0.6 Hz, 1H), 7.26 - 7.20 (m, 1H), 5.29 (s, 2H), 3.57 (s, 2H), 2.28 (s, 3H).

Intermediate d14: 4-Methoxybenzyl 3-oxobutanoate

Light yellow oil (1.0 g, 43%).

1H-NMR (300 MHz, CDCl3) δ = 7.29 (d, J = 8.5 Hz, 2H), 6.88 (d, J = 8.5 Hz, 2H), 5.10 (s, 2H), 3.80 (s, 3H), 3.46 (s, 2H), 2.22 (s, 3H).

Intermediate d15: 2-((tert-Butoxycarbonyl)oxy)ethyl 3-oxobutanoate

Colorless oil (1.2 g, 46%).

1H-NMR (300 MHz, CDCl3) δ = 4.32 (dd, J = 19.8, 5.3 Hz, 4H), 3.47 (s, 2H), 2.27 (s, 2H), 1.49 (s, 9H).

Intermediate d16: tert-butyl 4-(((3-oxobutanoyl)oxy)methyl)piperidine-1-carboxylate Yellow oil (0.46 g, 67%).

1H-NMR (300 MHz, CDCl3) δ = 4.12 (d, J = 14.5 Hz, 2H), 4.00 (d, J = 6.5 Hz, 2H), 3.46 (s, 2H), 2.69 (t, J = 12.5 Hz, 2H), 2.27 (s, 3H), 1.82 (s, 1H), 1.68 (d, J = 13.6 Hz, 2H), 1.45 (s, 9H), 1.31 - 1.05 (m, 2H).

Intermediate d17: Tetrahydro-2H-(pyran-4-yl)methyl-3-oxobutanoate

Colorless oil (0.58 g, 83%).

1H-NMR (300 MHz, CDCl3) δ = 4.05 - 3.92 (m, 4H), 3.47 (d, J = 0.4 Hz, 2H), 3.39 (td, J = 11.9, 2.3 Hz, 2H), 2.27 (s, 3H), 2.03 - 1.83 (m, 1H), 1.63 (ddd, J = 12.8, 3.9, 1.9 Hz, 2H), 1.37 (dtd, J = 13.3, 11.8, 4.5 Hz, 2H).

Intermediate d18: Cyclohexyl 3-oxobutanoate

Colorless oil (1.08 g, 84%).

1H-NMR (300 MHz, CDCl3) δ = 4.81 (td, J = 8.8, 3.8 Hz, 1H), 3.42 (s, 2H), 2.26 (s, 3H), 1.91 - 1.80 (m, 2H), 1.77 - 1.66 (m, 2H), 1.61 - 1.49 (m, 2H), 1.47 - 1.29 (m, 4H).

Intermediate d19: Tetrahydro-2H-(pyran-4-yl)-3-oxobutanoate

Colorless oil (0.6 g, 92%).

1H-NMR (300 MHz, CDCl3) δ = 5.07 - 4.96 (m, 1H), 3.90 (dt, J = 11.9, 4.6 Hz, 2H), 3.54 (ddd, J = 11.9, 8.9, 3.0 Hz, 2H), 3.46 (s, 2H), 2.27 (s, 3H), 1.97 - 1.91 (m, 2H), 1.70 (dtd, J = 13.0, 8.9, 4.0 Hz, 2H).

Intermediate d20: Cyclopropylmethyl 3-oxobutanoate

Colorless oil (0.9 g, 82%).

1H-NMR (300 MHz, CDCl3) δ = 3.97 (d, J = 7.3 Hz, 2H), 3.46 (s, 2H), 2.28 (s, 3H), 1.23 - 1.06 (m, 1H), 0.63 - 0.52 (m, 2H), 0.33 - 0.25 (m, 2H).

Intermediate d21: tert-Butyl 4-(((3-oxobutanoyl)oxy)methyl)piperidine-1-carboxylate Colorless oil (0.45 g, 71%).

1H-NMR (300 MHz, CDCl3) δ = 4.13 (bs, 2H), 4.00 (d, J = 6.5 Hz, 2H), 3.46 (s, 2H), 2.69 (t, J = 12.7 Hz, 2H), 2.27 (s, 3H), 1.82 (tdd, J = 15.2, 6.5, 3.5 Hz, 1H), 1.68 (d, J = 12.7 Hz, 2H), 1.45 (s, 9H), 1.19 (dtd, J = 12.7, 12.3, 5.7 Hz, 2H).

Intermediate d22: phenethyl 3-oxobutanoate

Yellow oil (0.97 g, 58%).

1H-NMR (300 MHz, CDCl3) δ = 7.34 - 7.27 (m, 2H), 7.25 - 7.17 (m, 3H), 4.37 (t, J = 7.0 Hz, 2H), 3.43 (s, 2H), 2.97 (t, J = 7.0 Hz, 2H), 2.20 (s, 3H).

Intermediate d23: 1,3-dicyclopropylpropane-1,3-dione

Colorless oil (1.3 g, 87%).

1H-NMR (300 MHz, CDCl3) δ = 3.78 (s, 2H), 1.56 (td, J = 8.0, 4.1 Hz, 2H), 1.15 - 1.03 (m, 4H), 0.98 - 0.85 (m, 4H).

Intermediate d24: methyl 3-cyclopropyl-3-oxopropanoate

Light-yellow oil (2.01 g, 79%).

1H-NMR (300 MHz, CDCl3) δ = 3.74 (d, J = 1.5 Hz, 3H), 3.58 (d, J = 1.2 Hz, 2H), 2.17 - 1.95 (m, 1H), 1.28 - 1.07 (m, 2H), 1.07 - 0.82 (m, 2H).

Intermediate d25: methyl 4-fluoro-3-oxobutanoate

Light-yellow oil (2.8 g, 78%).

1H-NMR (300 MHz, CDCl3) δ = 4.91 (d, J = 47.5 Hz, 2H), 3.76 (s, 3H), 3.61 (d, J = 3.7 Hz, 2H). 19F NMR (282 MHz, CDCl3) δ = 123.96 (t, J = 47.5 Hz).

Intermediate d26: Methyl 4-(benzyloxy)-3-oxobutanoate

Yellow oil (0.75 g, 51%).

1H-NMR (300 MHz, CDCl3) δ = 7.41 - 7.29 (m, 5H), 4.59 (s, 2H), 4.14 (s, 2H), 3.71 (s, 3H), 3.56 (s, 2H).

Intermediate d27: Methyl 3-oxo-4-phenoxybutanoate

Yellow oil (47%).

1H-NMR (300 MHz, CDCl3) δ = 7.53 - 7.31 (m, 5H), 4.32 (s, 2H), 3.69 (s, 3H), 3.55 (s, 2H).

Intermediate d28: 3-oxo-N-phenylbutanamide

Yellow oil (0.98 g, 79%).

1H-NMR (300 MHz, CDCl3) δ = 9.08 (bs, 1H), 7.54 (dt, J = 8.8, 1.7 Hz, 2H), 7.38 - 7.28 (m, 2H), 7.12 (tt, J = 7.1, 1.1 Hz, 1H), 3.59 (s, 2H), 2.33 (s, 3H).

Intermediate d29: tert-butyl 4-((3-oxobutanoyl)oxy)piperidine-1-carboxylate

Light yellow oil (1.3 g, 89%).

1H-NMR (300 MHz, CDCl3) δ = 4.99 (tt, J = 8.5, 3.6 Hz, 1H), 3.77 - 3.61 (m, 2H), 3.46 (s, 2H), 3.24 (ddd, J = 13.3, 8.5, 3.6 Hz, 2H), 2.27 (s, 3H), 1.87 (ddt, J = 12.9, 6.5, 3.4 Hz, 2H), 1.63 (dp, J = 12.9, 4.3 Hz, 2H), 1.46 (s, 9H).

Intermediate d30: Cyclopentylmethyl 3-oxobutanoate

Colorless oil (1.63 g, 84%).

1H-NMR (300 MHz, CDCl3) δ = 4.03 (d, J = 7.1 Hz, 2H), 3.45 (s, 2H), 2.27 (s, 3H), 2.24 - 2.15 (m, 1H), 1.81 - 1.69 (m, 2H), 1.64 - 1.51 (m, 4H), 1.34 - 1.17 (m, 2H).

Intermediate d31: 1-Methylpiperidin-4-yl 3-oxobutanoate

Yellow oil (1.2 g, 57%).

1H-NMR (300 MHz, CDCl3) δ = 4.84 (tt, J = 7.9, 3.8 Hz, 1H), 3.44 (s, 2H), 2.70 - 2.55 (m, 2H), 2.34 - 2.18 (m, 8H), 2.02 - 1.86 (m, 2H), 1.73 (dtd, J = 12.7, 8.6, 3.8 Hz, 2H).

Intermediate d32: Cyclopentyl 3-oxobutanoate

Colorless oil (1.25 g, 70%).

1H-NMR (300 MHz, CDCl3) δ = 5.28 - 5.16 (m, 1H), 3.40 (s, 2H), 2.26 (s, 3H), 1.92-1.81 (m, 2H), 1.76-1.66 (m, 4H), 1.63-1.54 (m, 2H).

Intermediate d33: 4,4-Dimethylcyclohexyl 3-oxobutanoate

Light yellow oil (0.42 g, 57%).

1H-NMR (300 MHz, CDCl3) δ = 4.80 (tt, J = 8.8, 4.1 Hz, 1H), 3.42 (s, 2H), 2.27 (s, 3H), 1.82 - 1.70 (m, 2H), 1.65 - 1.52 (m, 2H), 1.48 - 1.38 (m, 2H), 1.25 (ddd, J = 14.0, 10.4, 4.1 Hz, 2H), 0.93 (s, 3H), 0.91 (s, 3H).

Intermediate d34: Cyclobutyl 3-oxobutanoate

Colorless oil (1.06 g, 51%).

1H-NMR (300 MHz, CDCl3) δ = 5.09 - 4.96 (m, 1H), 3.41 (s, 2H), 2.42 - 2.29 (m, 2H), 2.26 (s, 3H), 2.14 - 2.00 (m, 2H), 1.86 - 1.54 (m, 2H).

Intermediate d35: 4-Fluorobenzyl 3-oxobutanoate

Colorless oil (1.32 g, 89%).

1H-NMR (300 MHz, CDCl3) δ = 7.41 - 7.28 (m, 2H), 7.13 - 6.96 (m, 2H), 5.14 (s, 2H), 3.49 (s, 2H), 2.24 (s, 3H). 19F NMR (282 MHz, CDCl3) δ = -113.31.

Intermediate d36: Pyridin-3-ylmethyl 3-oxobutanoate

Light-yellow oil (0.95 g, 70%).

1H-NMR (300 MHz, CDCl3) δ = 8.62 (d, J = 2.2 Hz, 1H), 8.59 (dd, J = 4.8, 1.6 Hz, 1H), 7.76 - 7.65 (m, 1H), 7.31 (ddd, J = 7.9, 4.8, 0.9 Hz, 1H), 5.19 (s, 2H), 3.51 (s, 2H), 2.25 (s, 3H).

Intermediate d37: tert-butyl 4-(((3-oxobutanoyl)oxy)methyl)benzoate

Light-yellow solid (0.59 g, 79%).

1H-NMR (300 MHz, CDCl3) δ = 7.96 (d, J = 8.0 Hz, 2H), 7.39 (d, J = 8.0 Hz, 2H), 5.15 (s, 2H), 1.93 (s, 2H), 1.93 (s, 3H), 1.59 (s, 9H).

Intermediate d38: 4-(Cyclopropylcarbamoyl)benzyl 3-oxobutanoate

General procedure d using N-cyclopropyl-4-(hydroxymethyl)benzamide (J.Med. Chem., 2001, 44, 1491-1508) yielded yellow oil (0.14 g, 76%).

1H-NMR (300 MHz, CDCl3) δ = 7.73 (d, J = 8.2 Hz, 2H), 7.40 (d, J = 8.2 Hz, 2H), 6.23 (bs, 1H), 5.20 (s, 2H), 3.52 (s, 2H), 2.90 (dq, J = 7.2, 3.5 Hz, 1H), 2.25 (s, 3H), 0.88 (q, J = 6.5 Hz, 2H), 0.67 - 0.52 (m, 2H).

Intermediate d39: 4-bromobenzyl 3-oxobutanoate

Yellow oil (0.5 g, 34%).

1H-NMR (300 MHz, CDCl3) δ = 7.49 (d, J = 8.0 Hz, 2H), 7.23 (d, J = 8.0 Hz, 2H), 5.12 (s, 2H), 3.50 (s, 2H), 2.24 (s, 3H).

Intermediate d40: 3-bromobenzyl 3-oxobutanoate

Yellow oil (1.5 g, 69%).

1H-NMR (300 MHz, CDCl3) δ = 7.50 (d, J = 1.8 Hz, 1H), 7.46 (dt, J = 7.5, 1.8 Hz, 1H), 7.29 - 7.21 (m, 2H), 5.13 (s, 2H), 3.51 (s, 2H), 2.25 (s, 3H).

Intermediate d41: 2-bromobenzyl 3-oxobutanoate

Yellow oil (1.2 g, 83%).

1H-NMR (300 MHz, CDCl3) δ = 7.56 (dd, J = 7.8, 1.1 Hz, 1H), 7.41 (dd, J = 7.5, 1.6 Hz, 1H), 7.31 (td, J = 7.5, 1.1 Hz, 1H), 7.18 (td, J = 7.8, 1.6 Hz, 1H), 5.25 (s, 2H), 3.52 (s, 2H), 2.26 (s, 3H).

Intermediate d42: (3-Fluoropyridin-4-yl)methyl 3-oxobutanoate Yellow oil (0.305 g, 41%).

1H-NMR (300 MHz, CDCl3) δ = 8.54 - 8.38 (m, 2H), 7.46 - 7.31 (m, 1H), 5.29 (s, 2H), 3.57 (s, 2H), 2.29 (s, 3H). 19F NMR (282 MHz, CDCl3) δ = -132.13 (s, CF).

Intermediate d43: Pyrimidin-5-ylmethyl 3-oxobutanoate

Yellow oil (0.65 g, 96%).

1H-NMR (300 MHz, CDCl3) δ = 9.20 (s, 1H), 8.77 (s, 2H), 5.20 (s, 2H), 3.53 (s, 2H), 2.26 (s, 3H).

Intermediate d44: (5-Bromopyridin-3-yl)methyl 3-oxobutanoate Yellow oil (0.5 g, 63%).

1H-NMR (300 MHz, CDCl3) δ = 8.65 (d, J = 2.0 Hz, 1H), 8.53 (s, 1H), 7.87 (s, 1H), 5.17 (s, 2H), 3.53 (s, 2H), 2.26 (s, 3H).

Intermediate d45: 3-cyanobenzyl 3-oxobutanoate

Yellow oil (0.57 g, 75%).

1H-NMR (300 MHz, CDCl3) δ = 7.68 - 7.57 (m, 3H), 7.53 - 7.45 (m, 1H), 5.20 (s, 2H), 3.54 (s, 2H), 2.27 (s, 3H).

Intermediate d46: 4-cyanobenzyl 3-oxobutanoate

Yellow oil (0.62 g, 82%).

1H-NMR (300 MHz, CDCl3) δ = 7.66 (d, J = 8.1 Hz, 2H), 7.46 (d, J = 8.1 Hz, 2H), 5.22 (s, 2H), 3.54 (s, 2H), 2.26 (s, 3H).

Intermediate d47: (6-chloropyridin-3-yl)methyl 3-oxobutanoate Yellow oil (0.69 g, 87%).

1H-NMR (300 MHz, CDCl3) δ = 8.31 (s, 1H), 7.79 - 7.52 (m, 1H), 7.26 (d, J = 8.2 Hz, 1H), 5.09 (s, 2H), 3.45 (s, 2H), 2.17 (s, 3H).

Intermediate d48: 3-morpholinobenzyl 3-oxobutanoate

Yellow oil (0.71 g, 92%).

1H-NMR (300 MHz, CDCl3) δ = 7.30 (d, J = 7.9 Hz, 1H), 6.94 - 6.86 (m, 3H), 5.16 (s, 2H), 3.90 - 3.86 (m, 4H), 3.51 (s, 2H), 3.21 - 3.17 (m, 4H), 2.27 (s, 3H).

Intermediate d49: (2-Chloropyridin-4-yl)methyl 3-oxobutanoate

Yellow oil (0.50 g, 63%).

1H-NMR (300 MHz, CDCl3) δ = 8.41 - 8.33 (m, 1H), 7.32 (s, 1H), 7.19 (d, J = 5.1 Hz, 1H), 5.17 (s, 2H), 3.58 (s, 2H), 2.28 (s, 3H).

Intermediate d50: 4,4-Difluorocyclohexyl 3-oxobutanoate

Yellow oil (0.35 g, 91%).

1H-NMR (300 MHz, CDCl3) δ = 4.93 (s, 1H), 4.53 (s, 2H), 2.04 (d, J = 1.1 Hz, 3H), 1.89 - 1.78 (m, 8H). 19F NMR (282 MHz, CDCl3) δ = -94.48 (d, J = 252.2 Hz, CF), -100.51 (d, J = 235.8 Hz, CF).

Intermediate d51: N-benzyl-N-methyl-3-oxobutanamide

Yellow oil (2.50 g, 86%).

1H-NMR (300 MHz, CDCl3) δ = 7.38 - 7.23 (m, 5H), 4.56 (d, J = 32.5 Hz, 3H), 3.60 (d, J = 12.5 Hz, 2H), 2.89 (s, 2H), 2.29 (s, 3H).

Intermediate d52: 4-chlorobenzyl 3-oxobutanoate

Yellow oil (0.5 g, 34%).

1H-NMR (300 MHz, CDCl3) δ = 7.49 (d, J = 8.0 Hz, 2H), 7.23 (d, J = 8.0 Hz, 2H), 5.12 (s, 2H), 3.50 (s, 2H), 2.24 (s, 3H).

Intermediate d53: 3-chlorobenzyl 3-oxobutanoate

Yellow oil (0.119 g, 75 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 7.35 (s, 1H), 7.33 - 7.27 (m, 2H), 7.25 - 7.19 (m, 1H), 5.14 (s, 2H), 3.52 (s, 2H), 2.26 (s, 3H).

Intermediate d54: 2-phenylpropan-2-yl 3-oxobutanoate

Modified procedure d using 4-methyleneoxetan-2-one (1 eq) and sodium acetate (0.1 eq) yielded the title compound as yellow oil (2.46 g, 76%).
1H-NMR (300 MHz, CDCl3) δ = 7.41 - 7.27 (m, 5H), 3.42 (s, 2H), 2.25 (s, 3H), 1.80 (s, 6H).

Intermediate d55: 2-(4-Fluorophenyl)propan-2-yl 3-oxobutanoate

Modified procedure d using 4-methyleneoxetan-2-one (1 eq) yielded the title compound as yellow oil (0.52 g, 34 %).
1H-NMR (300 MHz, CDCl3-d) δ (ppm): 7.40 - 7.31 (m, 2H), 7.05 - 6.95 (m, 2H), 3.41 (s, 2H), 2.23 (s, 3H), 1.78 (s, 6H).
19F NMR (282 MHz, CDCl3-d) δ (ppm): -115.79 (CF).

Intermediate d56: 2H-thiopyran-3,5(4H,6H)-dione

Synthesis according to literature procedure J. Org.Chem. 1977, 42, 1163-1169 yielded yellow oil (2.1 g, 52%).
1H-NMR (300 MHz, CDCl3) δ = 3.57 (s, 2H), 3.39 (s, 4H).

Intermediate d57: 3-((4-Methylpiperazin-1-yl)methyl)benzyl 3-oxobutanoate Step 1: Synthesis of 3-((4-methylpiperazin-1-yl)methyl)benzoate

To a solution of 1-methylpiperazine (3.0 mL, 26.89 mmol, 3.08 eq.) in acetone (35 ml) was added potassium carbonate (2.41 g, 17.46 mmol, 2 eq). The mixture was stirred for 10 minutes. Then methyl 3-bromobenzoate (2.0 g, 8.73 mmol, 1.0 eq.) was added, and the mixture was stirred at room temperature overnight. The solid was removed by filtration, and the filtrate was concentrated and purified by flash column chromatography on silica gel using dichloromethane / 3% methanol as eluent to afford methyl 3-((4-methylpiperazin-1-yl)methyl)benzoate as a yellow oil (2.1 g, 97%).
1H-NMR (300 MHz, CDCl3-d) δ (ppm): 7.97 (s, 1H), 7.92 (dt, J = 7.7, 1.4 Hz, 1H), 7.53 (d, J = 7.7 Hz, 1H), 7.38 (t, J = 7.7 Hz, 1H), 3.91 (s, 3H), 3.55 (s, 2H), 2.48 (bs, 8H), 2.29 (s, 3H).

Step 2: Synthesis of (3-((4-methylpiperazin-1-yl)methyl)phenyl)metanol

To a solution of methyl 3-((4-methylpiperazin-1-yl)methyl)benzoate (2.02 g, 8.13 mmol, 1 eq.) in tetrahydrofurane (60 ml) was added under vigorous stirring diisobutylaluminium hydride ( 18.73 mL, 18.73 mmol, 2.26 eq). The mixture was stirred at room temperature for 5 hours. Then Rochele's salt solution (105 mL) and dichloromethane (105 mL) were added. The mixture was stirred at room temperature for 60 minutes and then extracted with dichloromethane (x3) and dried (Na2SO4). The solvent was removed under reduced pressure, and the resulting residue was purified by flash column chromatography on silica gel using dichloromethane / 7% methanol as eluent to afford (3-((4-methylpiperazin-1-yl)methyl)phenyl)methanol as a yellow oil (1.75 g, 98%).
1H-NMR (300 MHz, CDCl3-d) δ (ppm): 7.32 - 7.15 (m, 4H), 4.62 (s, 2H), 3.71 (t, J = 6.2 Hz, 1H), 3.45 (s, 2H), 2.37 (bs, 8H), 2.20 (s, 3H).

Step 3: Synthesis of 3-((4-Methylpiperazin-1-yl)methyl)benzyl 3-oxobutanoate

Transformation of (3-((4-methylpiperazin-1-yl)methyl)phenyl)methanol using General procedure d yielded the title compound as a yellow oil (0.55 g, 81%). 1H-NMR (300 MHz, CDCl3-d) δ (ppm): 7.36 - 7.28 (m, 3H), 7.24 - 7.20 (m, 1H), 5.17 (s, 2H), 3.52 (s, 2H), 3.50 (s, 2H), 2.53 (bs, 8H), 2.34 (s, 3H), 2.25 (s, 3H).

Intermediate d58: Cyclopropyl 3-oxobutanoate

Modified procedure d using 4-methyleneoxetan-2-one (1 eq) and sodium acetate (0.1 eq) yielded the title compound as a yellow oil (1.99 g, 81 %).

1H-NMR (300 MHz, CDCl3) δ = 4.19 - 4.04 (m, 1H), 3.39 (s, 2H), 2.22 (s, 3H), 0.79 - 0.58 (m, 4H).

Intermediate d59: Prop-2-yn-1-yl 3-oxobutanoate

General procedure d yielded the title compound as a yellow oil (2.5 g, 91 %).

1H-NMR (300 MHz, CDCl3) δ = 4.74 (d, J = 2.5 Hz, 2H), 3.50 (s, 2H), 2.50 (t, J = 2.5 Hz, 1H), 2.28 (s, 3H).

Intermediate d60: But-2-yn-1-yl 3-oxobutanoate

General procedure d yielded the title compound as a yellow oil (3.0 g, 92 %).

1H-NMR (300 MHz, CDCl3) δ = 4.65 (q, J = 2.2 Hz, 2H), 3.44 (s, 2H), 2.22 (s, 3H), 1.80 (t, J = 2.2 Hz, 3H).

Intermediate d61: 2,2,2-Trifluoroethyl 3-oxobutanoate

General procedure d yielded the title compound as a yellow oil (2.1 g, 81 %).

1H-NMR (300 MHz, CDCl3) δ = 4.53 (q, J = 7.6, 6.9 Hz, 2H), 3.57 (s, 2H), 2.29 (s, 3H).

General procedure e Intermediate e1: Oxetan-3-yl 3-cyclopropyl-3-oxopropanoate

To a solution of methyl 3-cyclopropyl-3-oxopropanoate (1 g, 7.03 mmol, 1.2 eq.) in toluene (5 ml) was added oxetan-3-ol (0.37 ml, 5.87 mmol, 1 eq). The resultant mixture was refluxed using a Dean-Stark trap for 12 hours, cooled to RT and concentrated. The resulting residue was purified by flash column chromatography on silica gel using ethyl acetate/hexane (1:3) as eluent to afford the title compound as a light-yellow oil (0.52 g, 44%).
1H-NMR (300 MHz, CDCl3) δ = 5.50 (p, J = 5.8 Hz, 1H), 4.99-4.81 (m, 2H), 4.66 (dd, J = 7.8, 5.8 Hz, 2H), 3.63 (s, 2H), 2.02 (tt, J = 7.8, 4.5 Hz, 1H), 1.17-1.08 (m, 2H), 1.05-0.93 (m, 2H).

Intermediate e2: Isopropyl 3-cyclopropyl-3-oxopropanoate

General procedure e yielded the title compound as a light-yellow oil (0.9 g, 75%). 1H-NMR (300 MHz, CDCl3) δ = 5.06 (hept, J = 6.4 Hz, 1H), 3.52 (s, 2H), 2.14 - 1.93 (m, 1H), 1.26 (s, 3H), 1.24 (s, 3H), 1.15 - 1.06 (m, 2H), 1.00 - 0.90 (m, 2H).

Intermediate e3: Benzyl 4,4,4-trifluoro-3-oxobutanoate

General procedure e yielded the title compound as a red oil (1.8 g, 62%).

1H-NMR (300 MHz, CDCl3) δ = 7.37 (s, 5H), 5.24 (s, 2H), 2.86 (s, 2H). 19F NMR (282 MHz, CDCl3) δ = -87.15.

Intermediate e4: 2-Phenylpropan-2-yl 3-cyclopropyl-3-oxopropanoate

General procedure e using 1 eq of DMAP yielded the title compound as a yellow oil (0.41 g, 29 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 7.40 - 7.31 (m, 4H), 7.23 (d, J = 6.9 Hz, 1H), 3.53 (s, 2H), 2.03 - 1.99 (m, 1H), 1.80 (s, 6H), 1.15 - 1.09 (m, 2H), 1.00 - 0.89 (m, 2H).

Intermediate e5: Cyclohexyl 3-cyclopropyl-3-oxopropanoate

General procedure e yielded the title compound as a light-yellow solid (1.2 g, 85 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 4.91 - 4.75 (m, 1H), 3.52 (s, 2H), 2.12 - 1.95 (m, 1H), 1.93 - 1.79 (m, 2H), 1.78 - 1.60 (m, 2H), 1.53 - 1.23 (m, 6H), 1.18 - 1.04 (m, 2H), 1.03 - 0.88 (m, 2H).

Intermediate e6: 3-Chlorobenzyl 3-cyclopropyl-3-oxopropanoate

General procedure e yielded the title compound as a yellow oil (0.69 g, 78 %).
1H-NMR (300 MHz, CDCl3-d) δ (ppm): 7.35 (s, 1H), 7.32 - 7.27 (m, 2H), 7.25 - 7.20 (m, 1H), 5.15 (s, 2H), 3.63 (s, 2H), 2.09 - 1.92 (m, 1H), 1.20 - 1.05 (m, 2H), 1.03 - 0.87 (m, 2H).

Intermediate e7: 2-(4-Fluorophenyl)propan-2-yl 3-cyclopropyl-3-oxopropanoate

General procedure e yielded the title compound as a yellow oil (0.52 g, 35 %).
1H-NMR (300 MHz, CDCI -d) δ (ppm): 7.40 - 7.31 (m, 2H), 7.05 - 6.96 (m, 2H), 3.53 (s, 2H), 2.01 (tt, J = 7.7, 4.4 Hz, 1H), 1.78 (s, 6H), 1.12 (pd, J = 3.5, 1.4 Hz, 2H), 0.96 (dt, J = 7.7, 3.5 Hz, 2H).
19F NMR (282 MHz, CDCI -d) δ (ppm): -115.92 (CF).

Intermediate e8: Cyclopropylmethyl 3-cyclopropyl-3-oxopropanoate

General procedure e using 1 eq DMAP yielded the title compound as a light-yellow oil (2.2 g, 34 %).
1H-NMR (300 MHz, CDCl3-d) δ (ppm) 3.98 (d, J = 7.3 Hz, 2H), 3.58 (s, 2H), 2.05 (tt, J = 7.9, 4.6 Hz, 1H), 1.24 - 1.04 (m, 3H), 1.02 - 0.91 (m, 2H), 0.61 - 0.51 (m, 2H), 0.29 (dt, J = 6.4, 4.6 Hz, 2H).

Intermediate e): 3-Bromobenzyl 3-cyclopropyl-3-oxopropanoate

General procedure e yielded the title compound as a yellow oil (0.28 g, 27 %).
1H-NMR (300 MHz, CDCl3-d) δ (ppm): 7.51 (s, 1H), 7.45 (dt, J = 7.5, 1.7 Hz, 1H), 7.31 - 7.21 (m, 2H), 5.14 (s, 2H), 3.63 (s, 2H), 2.01 (tt, J = 7.7, 4.5 Hz, 1H), 1.21 - 1.06 (m, 2H), 1.01 - 0.87 (m, 2H).

Intermediate e10: Cyclopentyl 3-cyclopropyl-3-oxopropanoate

General procedur e yielded the title compound as a light-yellow oil (0.54 g, 79 %).
1H-NMR (300 MHz, CDCI -d) δ (ppm): 5.22 (tt, J = 6.0, 2.7 Hz, 1H), 3.51 (s, 2H), 2.02 (tt, J = 7.8, 4.5 Hz, 1H), 1.92 - 1.78 (m, 2H), 1.77 - 1.65 (m, 4H), 1.64 - 1.53 (m, 2H), 1.16 - 1.04 (m, 2H), 0.95 (dt, J = 7.8, 3.2 Hz, 2H).

Intermediate e11: Cyclopentyl 2-cyanoacetate

General procedure e yielded the title compound as a light-yellow oil (2.34 g, 50 %).
1H-NMR (300 MHz, CDCl3-d) δ (ppm): 5.25 (td, J = 5.8, 2.9 Hz, 1H), 3.41 (s, 2H), 1.96 - 1.82 (m, 2H), 1.81 - 1.68 (m, 4H), 1.67 - 1.55 (m, 2H).

General procedure f

The synthetic method was adapted from Sabitha-G et al., Bismuth(III)Chloride-Catalyzed Highly Efficient Transeterification of β-Keto Esters, Helvetica Chimica Acta, Vol. 94. 2011.

Intermediate f1: Pyridin-4-ylmethyl 3-cyclopropyl-3-oxopropanoate

In a sealed flask, a mixture of 2.0 g of molecular sieves, 500 mg (4.58 mmol) of pyridin-4-ylmethanol, 0.83 mL (6.87 mmol) of methyl 3-cyclopropyl-3-oxopropanoate and 10% (0.45 mmol) of trichlorobismuthane in 10 mL of dry toluene was stirred at reflux for 36 hours. The reaction mixture was filtered and the filtrate concentrated, the remaining solid was purified by silica gel chromatography column (cyclohexane/ethyl acetate) to yield the desired β-keto ester (455 mg, 45%).
1H-NMR (400 MHz, DMSO-d6) δ = 0.94 (m, 4H), 2.12 (ddd, 1H), 3.86 (s, 2H), 5.20 (s, 2H), 7.36 (d, 2H), 8.56 (d, 2H).
HPLC-MS: Rt 3.040; m/z 220.1 (MH+).

The following intermediates were synthesized according to General procedure f. Intermediate f2: 4-Fluorobenzyl 3-cyclopropyl-3-oxopropanoate
1H-NMR (400 MHz, DMSO-d6) δ = 0.91 (m, 4H), 2.08 (m, 1H), 3.77 (s, 2H), 5.12 (s, 2H), 7.21 (t, 2H), 7.43 (dd, 2H).

Intermediate f3: 4-Cyanobenzyl 3-cyclopropyl-3-oxopropanoate

1H-NMR (400 MHz, DMSO-d6) δ = 0.93 (m, 4H), 2.10 (ddd, 1H), 3.83 (s, 2H), 5.24 (s, 2H), 7.56 (d, 2H), 7.86 (d, 2H).

HPLC-MS: Rt 4.133; m/z 244.0 (MH+).

General procedure g (adapted from M. A. Walker et al, Bioorg. Med. Chem. Lett. 2006, 16, 2920-2924)

An aromatic ketone (1 eq) was dissolved in THF (0.5 M) at room temperature, and potassium tert-butoxide (3 eq) was added. The mixture was stirred for 5 minutes before dimethyl carbonate (10 eq) was added. The reaction mixture was stirred at RT for 5 hours, concentrated and quenched by the addition of water. The mixture was extracted with dichloromethane (x3), and the combined organic layers were washed with brine, dried (Na2SO4), filtered and concentrated. The crude product was purified by flash column chromatography on silica gel using ethyl acetate/hexane as eluent to afford the corresponding dicarbonyl compound.

The following intermediates were synthesized according to General procedure g.

Intermediate g1: Methyl 3-oxo-3-phenylpropanoate

Yellow oil (1.11 g, 66%).

1H-NMR (300 MHz, CDCl3) δ = 7.95 (d, J = 7.8 Hz, 2H), 7.65 - 7.41 (m, 3H), 4.01 (s, 2H), 3.76 (s, 3H).

Intermediate g2: Methyl 3-oxo-3-(pyridin-3-yl)propanoate

Yellow oil (1.22 g, 83%).

1H-NMR (300 MHz, CDCl3) δ = 9.15 (dd, J = 2.3, 1 Hz, 1H), 8.81 (dd, J = 4.8, 1.7 Hz, 1H), 8.23 (ddd, J = 8.0, 2.3, 1.7 Hz, 1H), 7.45 (ddd, J = 8.0, 4.8, 0.9 Hz, 1H), 4.02 (s, 2H), 3.76 (s, 3H).

Intermediate g3: 1-Phenylpentane-2,4-dione

Yellow oil (1.11 g, 66%).

1H-NMR (300 MHz, CDCl3) δ = 7.37 - 7.26 (m, 5H), 3.59 (s, 4H), 2.02 (s, 3H).

Intermediate g4: 1,3-Dicyclopropylpropane-1,3-dione

Light-yellow oil (1.3 g, 87%).

1H-NMR (300 MHz, CDCl3) δ = 3.78 (s, 2H), 2.03 (td, J = 7.9, 3.9 Hz, 2H), 1.14 - 1.04 (m, 8H).

Intermediate g5: tert-Butyl 3-(3-methoxy-3-oxopropanoyl)azetidine-1-carboxylate

Yellow oil (0.4 g, 63%).

1H-NMR (300 MHz, CDCl3) δ = 4.11 - 3.98 (m, 4H), 3.73 (s, 3H), 3.66 - 3.53 (m, 1H), 3.47 (s, 2H), 1.42 (s, 9H).

Intermediate g6: 1-(Pyridin-3-yl)butane-1,3-dione

Yellow oil (0.20 g, 30%).

1H-NMR (300 MHz, CDCl3) δ = 15.95 (s, 1H), 9.05 (d, J = 2.1 Hz, 1H), 8.71 (dd, J = 4.8, 1.6 Hz, 1H), 8.14 (dt, J = 8.0, 2.0 Hz, 1H), 7.38 (ddd, J = 8.0, 4.9, 0.9 Hz, 1H), 6.17 (s, 1H), 2.21 (s, 3H).

Intermediate g7: Methyl 3-cyclobutyl-3-oxopropanoate Colorless oil (1.97 g, 62 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 3.71 (s, 3H), 3.45 - 3.31 (m, 3H), 2.32 - 2.06 (m, 4H), 2.05 - 1.74 (m, 2H).

Intermediate g8: Methyl 3-cyclopentyl-3-oxopropanoate Colorless oil (0.51 g, 67 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 3.73 (s, 3H), 3.50 (s, 2H), 2.98 (p, J = 7.9 Hz, 1H), 1.89 - 1.73 (m, 4H), 1.68 - 1.57 (m, 4H).

Intermediate g9: Methyl 3-cyclohexyl-3-oxopropanoate Colorless oil (1.14 g, 78 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 3.71 (s, 3H), 3.48 (s, 2H), 2.44 (tt, J = 11.1, 3.4 Hz, 1H), 1.91 - 1.83 (m, 2H), 1.84 - 1.71 (m, 2H), 1.44 - 1.13 (m, 6H).

Intermediate g10: (Z)-3-Amino-3-cyclopropylacrylonitrile

Colorless oil (1.3 g, 81 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 4.63 (bs, 2H), 3.78 (s, 1H), 1.54 - 1.35 (m, 1H), 1.00 - 0.81 (m, 2H), 0.81 - 0.60 (m, 2H).

General procedure h (adapted from S. Mitsuhashi et al, J. Am. Chem. Soc. 2008, 130, 4140).

Preparation of keto ethers:

To a suspension of sodium hydride (2.2 eq) in THF (0.5 M) at RT, an alcohol (1.1 eq) was added dropwise. The mixture was stirred for 5 min before methyl 4-chloro-3-oxobutanoate (1 eq) was dropwise added. This mixture was stirred for 30 min and then concentrated in vacuum. Water was added, and the pH was adjusted to 6-7 by the addition of 2N aq. HCl. The resulting mixture was extracted with dichloromethane (x2) and ethyl acetate (x2), and the combined organic layers were washed with brine, dried (Na2SO4), filtered and concentrated. The resulting residue was purified by flash column chromatography on silica gel using ethyl acetate/hexane as eluent to afford the corresponding dicarbonyl compound.

Preparation of keto amines:

Methyl 4-chloro-3-oxobutanoate (1 eq) was dissolved in acetone (0.5 M) at RT, and an amine (1.1 eq) was added. The mixture was stirred for 5 min before trimethylamine (2 eq) was added. This mixture was stirred at 100° C for 3h and then concentrated under vacuum. The residue was taken up in water and extracted with dichloromethane (x2) and ethyl acetate (x2). The combined organic layers were washed with brine, dried (Na2SO4), filtered and concentrated. The resulting residue was purified by flash column chromatography on silica gel using ethyl acetate/hexane as eluent to afford the corresponding dicarbonyl compound.

The following intermediates were synthesized according to General procedure h.

Intermediate h1: Methyl 4-methoxy-3-oxobutanoate

Colorless oil (0.9 g, 93%).

1H-NMR (300 MHz, CDCl3) δ = 4.10 (s, 2H), 3.75 (s, 3H), 3.50 (s, 2H), 3.40 (s, 3H).

Intermediate h2: Methyl 4-(dimethylamino)-3-oxobutanoate

Light-yellow oil (0.37 g, 35%).

1H-NMR (300 MHz, CDCl3) δ = 3.73 (s, 3H), 3.51 (s, 2H), 3.20 (s, 2H), 2.28 (s, 6H).

Intermediate h3: Methyl 4-morpholino-3-oxobutanoate

Light-yellow oil (0.5 g, 37%).

1H-NMR (300 MHz, CDCl3) δ = 3.76 - 3.78 (m, 7H), 3.51 (s, 2H), 3.25 (s, 2H), 2.55 - 2.43 (m, 4H).

PREPARATION OF ENAMINE INTERMEDIATES General procedure i Intermediate i1: Dimethyl (Z)-3-aminopent-2-enedioate

To a round bottom flask containing a dimethyl 3-oxopentanedioate (0.83 ml, 5.74 mmol, 1 eq.) in MeOH (10 ml) at room temperature was added ammonium bicarbonate (1.13 g, 14.35 mmol, 2.5 eq). The mixture was stirred at room temperature overnight and then concentrated. The resulting residue was purified by flash column chromatography on silica gel using ethyl acetate/hexane (1:3) as eluent to afford dimethyl (Z)-3-aminopent-2-enedioate as a yellow oil (0.8 g, 81%).

1H-NMR (300 MHz, CDCl3) δ = 7.81 (bs, 1H), 5.50 (bs, 1H). 4.58 (s, 1H), 3.73 (s, 3H), 3.65 (s, 3H), 3.15 (s, 2H).

The following intermediates were synthesized according to General procedure i.

Intermediate i2: Cyclopropylmethyl (Z)-3-aminobut-2-enoate

White solid (0.45 g, 53%).

1H-NMR (300 MHz, CDCl3) δ = 7.91 (bs, 2H), 4.56 (s, 1H), 3.88 (d, J = 7.2 Hz, 2H), 1.90 (s, 3H), 1.19 - 1.07 (m, 1H), 0.58 - 0.50 (m, 2H), 0.32 - 0.23 (m, 2H).

Intermediate i3: Methyl (Z)-3-amino-5-methoxypent-2-enoate

Light-yellow oil (0.93 g, 94%).

1H-NMR (300 MHz, CDCl3) δ = 7.83 (bs, 1H), 5.50 (bs, 1H), 4.49 (s, 1H), 3.63 (s, 3H), 3.59 - 3.54 (m, 2H), 3.36 (s, 3H), 2.37 (t, J = 5.7 Hz, 2H).

Intermediate i4: Methyl (Z)-3-amino-4-(benzyloxy)but-2-enoate

Orange oil (0.55 g, 76%).

1H-NMR (300 MHz, CDCl3) δ = 7.41 - 7.36 (m, 1H), 7.36 - 7.30 (m, 4H), 6.61 (bs, 1H), 5.27 (bs, 1H), 4.58 - 4.54 (m, 1H), 4.52 (s, 2H), 4.07 (d, J = 0.7 Hz, 2H), 3.66 (s, 3H).

Intermediate i5: Methyl (Z)-3-amino-4-phenoxybut-2-enoate

Yellow solid (0.11 g, 65%).

1H-NMR (300 MHz, CDCl3) δ = 7.35 - 7.27 (m, 2H), 7.05 - 6.87 (m, 3H), 5.86 (bs, 2H), 4.74 - 4.67 (m, 1H), 4.59 (d, J = 0.8 Hz, 2H), 3.68 (s, 3H).

Intermediate i6: 5-Amino-2H-thiopyran-3(6H)-one

Brown solid (0.05 g, 35%).

1H-NMR (300 MHz, CDCl3) δ = 6.97 (bs, 2H), 4.94 (s, 1H), 3.31 (s, 2H), 3.03 (s, 2H).

Intermediates i7 and i8: (Z)-4-amino-1-methoxypent-3-en-2-one and (Z)-4-amino-5-methoxypent-3-en-2-one

General procedure i yielded a mixture of the title compounds that were separated by flash chromatography.

i7: (Z)-4-amino-1-methoxypent-3-en-2-one; light yellow oil, 0.703 g, 74%.

1H-NMR (300 MHz, CDCl3) δ = 9.56 (bs, 1H), 5.72 (bs, 1H), 5.00 (s, 1H), 3.97 (s, 2H), 3.38 (s, 3H), 2.05 (s, 3H).

i8: (Z)-4-amino-5-methoxypent-3-en-2-one; light yellow oil, 0.25 g, 26%.

1H-NMR (300 MHz, CDCl3) δ = 9.85 (bs, 1H), 5.31 (s, 1H), 5.12 (bs, 1H), 3.89 (s, 2H), 3.41 (s, 3H), 1.97 (s, 3H).

Intermediate i9: Methyl (Z)-3-amino-4-fluorobut-2-enoate

Yellowish solid (0.22 g, 55%).

1H-NMR (300 MHz, CDCl3) δ = 6.35 (bs, 2H), 4.89 (d, J = 46.9 Hz, 2H), 4.59 (s, 1H), 3.67 (s, 3H). 19F NMR (282 MHz, CDCl3) δ = 122.24 (t, J = 46.9 Hz).

Intermediate i10: Cyclohexylmethyl (Z)-3-aminobut-2-enoate

Yellowish oil (0.8 g, 73%).

1H-NMR (300 MHz, CDCl3) δ = 7.88 (s, 2H), 4.53 (s, 1H), 3.86 (d, J= 6.6 Hz, 2H), 1.90 (s, 3H), 1.87 - 1.47 (m, 6H), 1.38 - 1.12 (m, 3H), 1.08 - 0.76 (m, 2H).

Intermediate i11: Cyclopentylmethyl (Z)-3-aminobut-2-enoate

Yellowish oil (0.3 g, 55%).

1H-NMR (300 MHz, CDCl3) δ = 7.92 (s, 2H), 4.54 (s, 1H), 3.93 (d, J = 7.1 Hz, 2H), 2.20 (p, J = 7.5 Hz, 1H), 1.90 (s, 3H), 1.74 (dtdd, J= 13.6, 7.5, 5.2, 3.4 Hz, 2H), 1.67 - 1.46 (m, 4H), 1.34 - 1.18 (m, 2H).

Intermediate i12: Cyclohexyl (Z)-3-aminobut-2-enoate

Yellowish oil (0.75 g, 63%).

1H-NMR (300 MHz, CDCl3) δ = 7.90 (s, 2H), 4.73 (tt, J = 8.8, 4.3 Hz, 1H), 4.52 (s, 1H), 2.27 (s, 3H), 1.72 (d, J = 10.3 Hz, 4H), 1.61 - 1.49 (m, 4H), 1.49 - 1.35 (m, 2H).

Intermediate i13: Cyclopentyl (Z)-3-aminobut-2-enoate

Yellowish oil (0.17 g, 24%).

1H-NMR (300 MHz, CDCl3) δ = 7.90 (s, 2H), 4.73 (tt, J = 8.8, 4.3 Hz, 1H), 4.52 (s, 1H), 2.27 (s, 3H), 1.72 (d, J = 10.3 Hz, 2H), 1.61 - 1.49 (m, 4H), 1.49 - 1.35 (m, 2H).

Intermediate i14: Benzyl (Z)-3-aminobut-2-enoate

Yellowish oil (0.203 g, 68%).

1H-NMR (300 MHz, CDCl3) δ = 7.91 (s, 2H), 7.36 - 7.28 (m, 5H), 5.12 (s, 2H), 4.61 (s, 1H), 1.91 (s, 3H).

Intermediate i15: (Tetrahydro-2H-pyran-4-yl)methyl (Z)-3-aminobut-2-enoate

Yellowish oil (0.23 g, 77%).

1H-NMR (300 MHz, CDCl3) δ = 7.85 (s, 2H), 4.51 (s, 1H), 4.05 - 3.85 (m, 4H), 3.37 (td, J = 11.8, 2.2 Hz, 2H), 1.96 - 1.86 (m, 4H), 1.62 (ddt, J = 10.8, 4.3, 2.2 Hz, 2H), 1.35 (dtd, J = 13.3, 11.8, 4.5 Hz, 2H).

Intermediate i16: (Tetrahydro-2H-pyran-4-yl)methyl (Z)-3-aminobut-2-enoate

Yellowish oil (0.23 g, 77%).

1H-NMR (300 MHz, CDCl3) δ = 7.85 (s, 2H), 4.51 (s, 1H), 4.05 - 3.85 (m, 4H), 3.37 (td, J = 11.8, 2.2 Hz, 2H), 1.96 - 1.86 (m, 4H), 1.62 (ddt, J = 10.8, 4.3, 2.2 Hz, 2H), 1.35 (dtd, J = 13.3, 11.8, 4.5 Hz, 2H).

Intermediate i17: Pyridin-4-ylmethyl (Z)-3-aminobut-2-enoate

Yellow oil (0.19 g, 69%).

1H-NMR (300 MHz, CDCl3) δ = 8.71 - 8.39 (m, 2H), 7.89 (s, 2H), 7.29 - 7.23 (m, 2H), 5.13 (s, 2H), 4.64 (s, 1H), 1.94 (s, 3H).

Intermediate i18: 4-Fluorobenzyl (Z)-3-aminobut-2-enoate

Yellow oil (0.72 g, 96%).

1H-NMR (300 MHz, CDCl3) δ = 7.96 (s, 2H), 7.40 - 7.30 (m, 2H), 7.11 - 6.89 (m, 2H), 5.07 (s, 2H), 4.58 (s, 1H), 1.97 - 1.81 (m, 3H).

Intermediate i19: tert-Butyl (Z)-4-(((3-aminobut-2-enoyl)oxy)methyl)benzoate

Yellow oil (0.40 g, 89%).

1H-NMR (300 MHz, CDCl3) δ = 7.98 - 7.92 (m, 2H), 7.42 - 7.33 (m, 2H), 5.15 (s, 2H), 4.61 (s, 1H), 1.92 (s, 3H), 1.58 (s, 9H) 1.28 (s, 2H).

Intermediate i20: Pyridin-3-ylmethyl (Z)-3-aminobut-2-enoate

Yellowish oil (0.50 g, 71%).

1H-NMR (300 MHz, CDCl3) δ = 8.62 (d, J = 1.7 Hz, 1H), 8.54 (dd, J = 4.8, 1.6 Hz, 1H), 7.94 (s, 2H), 7.70 (dt, J = 7.8, 1.9 Hz, 1H), 7.31 - 7.27 (m, 1H), 5.12 (s, 2H), 4.58 (s, 1H), 1.92 (s, 3H).

Intermediate i21: 4-Bromobenzyl (Z)-3-aminobut-2-enoate

Light-yellow oil (0.48 g, 95%).

1H-NMR (300 MHz, CDCl3) δ = 7.85 (s, 2H), 7.46 (d, J = 8.4 Hz, 2H), 7.23 (d, J = 8.4 Hz, 2H), 5.05 (s, 2H), 4.58 (s, 1H), 1.91 (s, 3H).

Intermediate i22: 3-Bromobenzyl (Z)-3-aminobut-2-enoate

Light-yellow oil (0.2 g, 69%).

1H-NMR (300 MHz, CDCl3) δ = 7.92 (s, 2H), 7.51 (s, 1H), 7.44 (m, 2H), 7.24 - 7.18 (m, 1H), 5.07 (s, 2H), 3.52 (s, 1H), 1.92 (s, 3H).

Intermediate i23: 2-Bromobenzyl (Z)-3-aminobut-2-enoate

Light-yellow oil (0.45 g, 90%).

1H-NMR (300 MHz, CDCl3) δ = 7.95 (bs, 2H), 7.61 - 7.44 (m, 1H), 7.41 (d, J = 7.6 Hz, 1H), 7.33 - 7.22 (m, 1H), 7.22 - 7.06 (m, 1H), 5.19 (s, 2H), 4.64 (s, 1H), 1.93 (s, 3H).

Intermediate i24: (3-Fluoropyridin-4-yl)methyl (Z)-3-aminobut-2-enoate

Light-yellow oil (0.207 g, 69%).

1H-NMR (300 MHz, CDCl3) δ = 8.55-8.30 (m, 2H), 7.92 (bs, 2H), 7.35 (t, J = 5.6 Hz, 1H), 5.22 (s, 2H), 4.64 (s, 1H), 1.95 (s, 3H). 19F NMR (282 MHz, CDCl3) δ = -132.71 (s, F).

Intermediate i25: Pyrimidin-5-ylmethyl (Z)-3-aminobut-2-enoate

Light-yellow oil (0.415 g, 64%).

1H-NMR (300 MHz, CDCl3) δ = 9.16 (s, 1H), 8.77 (d, J = 4.8 Hz, 2H), 7.94 (bs, 2H), 5.12 (s, 2H), 3.53 (s, 1H), 1.93 (s, 3H).

Intermediate i26: (5-Bromopyridin-3-yl)methyl (Z)-3-aminobut-2-enoate

Light-yellow oil (0.364 g, 73%).

1H-NMR (300 MHz, CDCl3) δ = 9.16 (d, J = 4.8 Hz, 1H), 8.77 (d, J = 4.8 Hz, 1H), 7.94 (s, 3H), 5.12 (s, 2H), 3.53 (s, 1H), 1.93 (s, 3H).

Intermediate i27: 2-Phenylpropan-2-yl (Z)-3-aminobut-2-enoate

Light-yellow oil (0.20 g, 69%).

1H-NMR (300 MHz, CDCl3) δ = 7.74 (bs, 1H), 7.41 - 7.29 (m, 5H), 4.61 (s, 1H), 4.34 (bs, 2H), 1.88 (s, 3H), 1.76 (s, 6H).

Intermediate i28: 3-cyanobenzyl (Z)-3-aminobut-2-enoate

Light-yellow oil (0.52 g, 93%).

1H-NMR (300 MHz, CDCl3) δ = 7.92 (bs, 2H), 7.66 (s, 1H), 7.57 (d, J = 7.9 Hz, 2H), 7.51 - 7.36 (m, 1H), 5.12 (s, 2H), 4.60 (s, 1H), 1.93 (s, 3H).

Intermediate i29: 4-cyanobenzyl (Z)-3-aminobut-2-enoate

Light-yellow oil (0.56 g, 90%).

1H-NMR (300 MHz, CDCl3) δ = 7.92 (bs, 2H), 7.67 - 7.61 (m, 2H), 7.44 (d, J = 7.9 Hz, 2H), 5.15 (s, 2H), 4.61 (s, 1H), 1.93 (s, 3H).

Intermediate i30: 4-cyanobenzyl (Z)-3-aminobut-2-enoate

Light-yellow oil (0.22 g, 75%).

1H-NMR (300 MHz, CDCl3) δ = 8.37 (s, 1H), 7.90 (s, 2H), 7.65 (dd, J = 8.2, 2.4 Hz, 1H), 7.30 (dd, J = 12.8, 8.2 Hz, 1H), 5.07 (s, 2H), 4.54 (s, 1H), 1.90 (s, 3H).

Intermediate i31: 3-Morpholinobenzyl (Z)-3-aminobut-2-enoate

Light-yellow oil (0.69 g, 97%).

1H-NMR (300 MHz, CDCl3) δ = 7.92 (s, 2H), 7.31 - 7.20 (m, 1H), 6.88 (dd, J = 16.4, 8.5 Hz, 3H), 5.07 (s, 2H), 4.60 (s, 1H), 3.86 (t, J = 4.8 Hz, 4H), 3.16 (t, J = 4.8 Hz, 4H), 1.91 (s, 3H).

Intermediate i32: 4,4-Dimethylcyclohexyl (Z)-3-aminobut-2-enoate

Light-yellow oil (0.55 g, 81%).

1H-NMR (300 MHz, CDCl3) δ = 7.85 (bs, 2H), 4.71 (tt, J = 8.9, 4.2 Hz, 1H), 4.52 (s, 1H), 1.90 (s, 3H), 1.82 - 1.70 (m, 2H), 1.58 (dt, J = 9.3, 5.8 Hz, 2H), 1.49 - 1.39 (m, 2H), 1.26 (ddd, J = 13.7, 10.8, 4.1 Hz, 2H), 0.93 (s, 3H), 0.91 (s, 3H).

Intermediate i33: (2-chloropyridin-4-yl)methyl (Z)-3-aminobut-2-enoate

Light-yellow oil (0.25 g, 83%).

1H-NMR (300 MHz, CDCl3) δ = 8.33 (d, J = 5.4 Hz, 1H), 7.92 (bs, 2H), 7.30 (s, 1H), 7.17 (d, J = 5.4 Hz, 1H), 5.10 (s, 2H), 4.64 (s, 1H), 1.95 (s, 3H).

Intermediate i34: 4,4-difluorocyclohexyl (Z)-3-aminobut-2-enoate

Light-yellow oil (0.30 g, 94%).

1H-NMR (300 MHz, CDCl3) δ = 7.86 (bs, 2H), 5.16 - 4.75 (m, 1H), 4.53 (s, 1H), 2.16 - 1.98 (m, 3H), 2 - 1,78 (m, 8H). 19F NMR (282 MHz, CDCl3) δ = -94.48 (d, J = 252.2 Hz), -100.51 (d, J = 235.8 Hz).

Intermediate i35: (Z)-3-Amino-N-benzyl-N-methylbut-2-enamide

Light-yellow oil (0.70 g, 71%).

1H-NMR (300 MHz, CDCl3) δ = 7.54 - 6.96 (m, 7H), 4.72 (s, 1H), 4.56 (m, 2H), 2.92 (s, 3H), 1.90 (s, 3H).

Intermediate i36: oxetan-3-yl (Z)-3-aminobut-2-enoate

Light-yellow oil (0.04 g, 17%).

1H-NMR (300 MHz, CDCl3) δ = 6.33 (s, 2H), 5.42 (tt, J = 6.4, 5.6 Hz, 1H), 4.87 (td, J = 6.8, 1.0 Hz, 2H), 4.66 (ddd, J = 6.9, 5.6, 1.0 Hz, 2H), 4.50 (s, 1H), 1.48 - 1.38 (m, 1H), 0.98 - 0.83 (m, 2H), 0.83 - 0.70 (m, 2H).

Intermediate i37: 3-Chlorobenzyl (Z)-3-aminobut-2-enoate

Light-yellow oil (0.198 g, 99 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 7.93 (bs, 1H), 7.36 (s, 1H), 7.33 - 7.26 (m, 1H), 7.24 (s, 2H), 5.08 (s, 2H), 4.89 - 4.39 (m, 2H), 1.93 (s, 3H).

Intermediate i38: 2-Phenylpropan-2-yl (Z)-3-amino-3-cyclopropylacrylate

Light-yellow solid (0.38 g, 93 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 7.40 - 7.31 (m, 4H), 7.24 - 7.17 (m, 1H), 6.19 (s, 2H), 4.56 (s, 1H), 1.76 (s, 6H), 1.47 - 1.31 (m, 1H), 0.87 - 0.79 (m, 2H), 0.78 - 0.70 (m, 2H).

Intermediate i39: Cyclohexyl (Z)-3-amino-3-cyclopropylacrylate

Light-yellow solid (0.22 g, 43 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 6.22 (bs, 2H), 4.80 - 4.58 (m, 1H), 4.46 (s, 1H), 1.97 - 1.77 (m, 2H), 1.80 - 1.61 (m, 2H), 1.56 - 1.18 (m, 7H), 0.90 - 0.78 (m, 2H), 0.78 - 0.68 (m, 2H).

Intermediate i40: (Z)-3-Amino-1,3-dicyclopropylprop-2-en-1-one

Light-yellow solid (0.22 g, 44 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 9.82 (bs, 1H), 5.12 (s, 1H), 4.83 (bs, 1H), 1.75 - 1.59 (m, 1H), 1.49 - 1.32 (m, 1H), 1.01 - 0.84 (m, 4H), 0.84 - 0.76 (m, 2H), 0.76 - 0.63 (m, 2H).

Intermediate i41: 3-Chlorobenzyl (Z)-3-amino-3-cyclopropylacrylate

Light-yellow solid (0.14 g, 70 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 7.37 - 7.29 (m, 1H), 7.23 - 7.17 (m, 3H), 6.25 (bs, 2H), 5.03 (s, 1H), 4.65 (s, 2H), 1.19 - 1.03 (m, 1H), 0.92 - 0.75 (m, 2H), 0.78 - 0.63 (m, 2H).

Intermediate i42: 2-(4-Fluorophenyl)propan-2-yl (Z)-3-aminobut-2-enoate

Light-yellow solid (0.20 g, 80 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 7.77 (bs, 1H), 7.39 - 7.27 (m, 2H), 7.08 - 6.93 (m, 2H), 4.58 (s, 1H), 4.36 (bs, 1H), 1.88 (s, 3H), 1.74 (s, 6H).

19F NMR (282 MHz, CDCl3-d) δ (ppm): -117.11 (CF).

Intermediate i43: 2-(4-Fluorophenyl)propan-2-yl (Z)-3-amino-3-cyclopropylacrylate

Yellow solid (0.43 g, 96 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 7.41 - 7.28 (m, 2H), 7.09 - 6.89 (m, 2H), 6.11 (bs, 2H), 4.54 (s, 1H), 1.73 (s, 6H), 1.40 (qt, J = 8.1, 5.0 Hz, 1H), 0.89 - 0.81 (m, 2H), 0.73 (dt, J = 8.1, 5.0 Hz, 2H).

19F NMR (282 MHz, CDCl3-d) δ (ppm): -117.14 (CF).

Intermediate i44: 3-Bromobenzyl (Z)-3-amino-3-cyclopropylacrylate

Light-yellow solid (0.15 g, 54 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 7.57 - 7.48 (m, 1H), 7.46 - 7.37 (m, 1H), 7.33 - 7.24 (m, 1H), 7.25 - 7.18 (m, 1H), 6.10 (bs, 2H), 5.06 (s, 1H), 3.75 (s, 2H), 2.10 - 1.93 (m, 1H), 1.18 - 1.03 (m, 2H), 1.03 - 0.92 (m, 2H).

Intermediate i45: Methyl (Z)-3-amino-3-cyclobutylacrylate

Light-yellow solid (0.35 g, 70 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 6.98 (bs, 2H), 4.53 (s, 1H), 3.64 (s, 3H), 3.05 (p, J = 8.4 Hz, 1H), 2.22 - 1.95 (m, 4H), 1.94 - 1.57 (m, 2H).

Intermediate i46: Methyl (Z)-3-amino-3-cyclopentylacrylate

Light-yellow solid (0.42 g, 83 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 7.52 (bs, 2H), 4.59 (s, 1H), 3.63 (s, 3H), 2.49 (p, J = 8.5 Hz, 1H), 2.07 - 1.85 (m, 2H), 1.85 - 1.40 (m, 6H).

Intermediate i47: Methyl (Z)-3-amino-3-cyclohexylacrylate

Light-yellow solid (0.45 g, 92 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 17.57 (bs, 2H), 4.54 (s, 1H), 3.63 (s, 3H), 1.98 - 1.60 (m, 6H), 1.35 - 1.13 (m, 5H).

Intermediate i48: 3-((4-Methylpiperazin-1-yl)methyl)benzyl (Z)-3-aminobut-2-enoate

Yellow solid (0.54 g, 98%).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 7.91 (s, 2H), 7.29 (s, 4H), 5.09 (s, 2H), 4.60 (s, 1H), 3.51 (s, 2H), 2.52 (bs, 8H), 2.33 (s, 3H), 1.91 (s, 3H).

Other methods of enamine preparation:

The following enamines have been prepared by alternative procedures:

Intermediate i49: 2,2,2-trifluoroethyl (Z)-3-aminobut-2-enoate

2,2,2-trifluoroethyl 3-oxobutanoate (1 g, 5.43 mmol, 1 eq) was dissolved in THF (1.2 M) at RT, and molecular sieves (2 g), ammonium acetate (0.83 g, 10.87 mmol, 2 eq) and acetic acid (0,310 ml, 5.43 mmol, 1 eq) were added. The reaction mixture was heated at 110 °C for 6 hours and then allowed to cool to RT. Water was addded, and the mixture was extracted with ethyl acetate (×3). The combined organic layers were washed with brine, dried (Na2SO4), filtered and concentrated. The residue was purified by flash column chromatography on silica gel using ethyl acetate/hexane (1:3) as eluent to afford 2,2,2-trifluoroethyl (Z)-3-aminobut-2-enoate (0.5 g, 56%).
1H-NMR (300 MHz, CDCl3) δ = 7.89 (bs, 1H), 4.84 (bs, 1H), 4.59 (s, 1H), 4.44 (q, J = 8.7 Hz, 2H), 1.93 (s, 3H). 19F NMR (282 MHz, CDCl3) δ = -73.40 (t, J = 8.6 Hz, 3F).

Intermediate i50: Methyl (Z)-3-amino-3-(pyridin-3-yl)acrylate

The procedure described for 2,2,2-trifluoroethyl (Z)-3-aminobut-2-enoate (see above) was followed to obtain the title compound (0.7 g, 72%).
1H-NMR (300 MHz, CDCl3) δ = 8.80 (s, 1H), 8.66 (s, 1H), 7.82 (d, J = 7.8 Hz, 1H), 7.40 - 7.30 (m, 1H), 4.96 (s, 1H), 3.72 (s, 3H).

Intermediate i51: Mixture of (Z)-4-amino-5-phenylpent-3-en-2-one and (Z)-4-amino-1-phenylpent-3-en-2-one

The procedure described for 2,2,2-trifluoroethyl (Z)-3-aminobut-2-enoate (see above) was followed to obtain a mixture of (Z)-4-amino-5-phenylpent-3-en-2-one and (Z)-4-amino-1-phenylpent-3-en-2-one as a yellow oil (0.5 g, 79%) that was used as such in the subsequent step.

Intermediate i52: (Z)-4-Aminopent-3-en-2-one

Prepared according to the process described in US 8,030,302 (3.7 g, 74%). 1H-NMR (300 MHz, CDCl3) δ = 9.70 (bs, 1H), 5.04 (bs, 1H), 4.92 (s, 1H), 2.03 (s, 3H), 1.91 (s, 3H).

Intermediate i53: 5-Amino-2H-pyran-3(6H)-one

Literature procedure Synth. Comm. 2004, 34, 557-565 yielded the title compound as a dark orange solid (0.35 g, 90%).
1H-NMR (300 MHz, CDCl3) δ = 7.03 (bs, 2H), 5.01 (s, 1H), 4.18 (s, 2H), 3.80 (s, 2H).

PREPARATION OF AMIDINE INTERMEDIATES General procedure y Intermediate y1: Cyclopentyl 3-amino-3-iminopropanoate hydrochloride Step 1: Synthesis of cyclopentyl 3-imino-3-phenoxypropanoate hydrochloride

A mixture of cyclopentyl 2-cyanoacetate (2.81 g, 18.34 mmol, 1.15 eq) and phenol (1.50 g, 15.95 mmol, 1.3 eq) was stirred at -20°C, when HCl gas was bubbled into the mixture for 90 min. Then, the mixture was left at 4°C for two days without stirring. Cold ether was added, and the mixture was stirred at 0°C. The solid formed and was filtered off and washed with cold ether to afford cyclopentyl 3-imino-3-phenoxypropanoate hydrochloride as a white solid (2.76 g, 61%).
1H-NMR (300 MHz, DMSO-d6) δ (ppm): 7.55 - 7.41 (m, 1H), 7.20 - 7.09 (m, 2H), 6.80 - 6.70 (m, 2H), 5.10 - 5.01 (m, 1H), 3.95 (s, 4H), 1.94 - 1.69 (m, 2H), 1.68 - 1.47 (m, 6H).

Step 2: Synthesis of cyclopentyl 3-imino-3-phenoxypropanoate

To a suspension of cyclopentyl 3-imino-3-phenoxypropanoate hydrochloride (1.0 g, 3.53 mmol, 1.0 eq.) in ether (25 ml) was added a solution of potassium carbonate (0.73 g, 5.30 mmol, 1.5 eq) in water (11 mL) at 0°C. The resultant mixture was stirred until the solid had dissolved and was then washed with ether, dried (Na2SO4) and concentrated to afford cyclopentyl 3-imino-3-phenoxypropanoate as light yellow oil (0.86 g, 99%).
1H-NMR (300 MHz, DMSO-d6) δ (ppm): 7.66 (bs, 2H), 7.51 - 7.39 (m, 2H), 7.35 - 7.23 (m, 1H), 7.20 - 7.10 (m, 2H), 4.97 (dq, J = 5.7, 3.1 Hz, 1H), 3.46 (s, 1H), 1.89 - 1.65 (m, 2H), 1.64 - 1.25 (m, 6H).

Step 3: Synthesis of cyclopentyl 3-amino-3-iminopropanoate hydrochloride

To a solution of cyclopentyl 3-imino-3-phenoxypropanoate (0.86 g, 3.49 mmol, 1.0 eq.) in methanol (5 ml) was added ammonium chloride (0.21 g, 3.84 mmol, 1.1 eq). The mixture was stirred at room temperature overnight and concentrated. The remaining solid was triturated with diethyl ether and filtered off to afford cyclopentyl 3-amino-3-iminopropanoate hydrochloride as a white solid (0.61 g, 84%).
1H-NMR (300 MHz, DMSO-d6) δ (ppm): 9.12 (bs, 2H), 8.82 (bs, 2H), 5.12 (tt, J = 4.9, 2.2 Hz, 1H), 3.55 (s, 2H), 1.81 (t, J = 7.5 Hz, 2H), 1.76 - 1.47 (m, 6H).

The cyclopentyl 2-cyanoacetate precursor used in Step 1 of the synthesis of Intermediate y1 was purchased from commercial vendors. Other alkyl 2-cyanoacetates were synthesized using the following ester formation method (Step 0), as shown for 3-fluorobenzyl 2-cyanoacetate, a precursor of Intermediate y2:

Step 0: Synthesis of 3-fluorobenzyl 2-cyanoacetate

To a solution of 2-cyanoacetic acid (4.45 g, 52.32 mmol, 1.0 eq.) in DCM (34 ml) was added (3-fluorophenyl)methanol (5.67 mL, 52.32 mmol, 1.0 eq) and cooled at 0°C. Then, a solution of N,N- dicylohexylcarbodiimide (10.79 g, 52.32 mmol, 1.0 eq) and dimethylaminopyridine (0.32 g, 2.62 mmol, 0.05 eq) in DCM (16 mL) was dropwise added, and the mixture was stirred at RT overnight. The solid was filtered off and washed with DCM. The filtrate was concentrated and purified by flash column chromatography on silica gel using ethyl acetate/hexane (1:3) as eluent to afford 3-fluorobenzyl 2-cyanoacetate as a light-yellow oil (7.2 g, 66%).
1H-NMR (300 MHz, DMSO-d6) δ (ppm): 7.45 - 7.27 (m, 1H), 7.20 - 7.01 (m, 3H), 5.22 (s, 2H), 3.51 (s, 2H).

The following amidine intermediates were prepared according to General Procedure y (Steps 0 to 3).

Intermediate y2: 3-Fluorobenzyl 3,3-diaminoacrylate hydrochloride

White solid (0.550 g).

1H-NMR (300 MHz, DMSO-d6) δ (ppm): 9.36 (bs, 2H), 9.05 (bs, 2H), 7.64 - 7.43 (m, 4H), 5.21 (s, 2H), 3.78 (s, 2H).

Intermediate y3: Cyclobutylmethyl 3,3-diaminoacrylate hydrochloride

White solid (0.542 g).

1H-NMR (300 MHz, DMSO-d6) δ (ppm): 9.27 (bs, 2H), 8.99 (bs, 2H), 4.19 - 3.85 (m, 2H), 3.67 (s, 2H), 2.67 - 2.53 (m, 1H), 2.11 - 1.54 (m, 6H).

Intermediate y4: 3,3-Difluorocyclobutyl)methyl 3,3-diaminoacrylate

White solid (0.450 g).

1H-NMR (300 MHz, DMSO-d6) δ (ppm): 9.33 (bs, 2H), 9.01 (bs, 2H), 4.17 (d, J = 5.1 Hz, 2H), 3.71 (s, 2H), 2.79 - 2.53 (m, 3H), 2.44 - 2.29 (m, 2H).

Intermediate y5: 2,2-Difluorocyclopropyl)methyl 3,3-diaminoacrylate hydrochloride White solid (0.36 g).

1H-NMR (300 MHz, DMSO-d6) δ (ppm): 9.33 (bs, 2H), 9.05 (bs, 2H), 4.42 - 4.21 (m, 1H), 4.14 - 3.90 (m, 1H), 3.73 (s, 2H), 2.20 - 2.00 (m, 1H), 1.82 - 1.61 (m, 1H), 1.61 - 1.36 (m, 1H).

Intermediate y6: Isopropyl 3,3-diaminoacrylate hydrochloride

White solid (0.47 g).

1H-NMR (300 MHz, DMSO-d6) δ (ppm): 9.28 (bs, 2H), 9.00 (bs, 2H), 4.95 (pd, J = 6.3, 1.5 Hz, 1H), 3.62 (s, 2H), 1.22 (d, J = 6.3 Hz, 6H).

Intermediate y7: 2,2-Difluoro-3,3-dimethylcyclopropyl)methyl 3,3-diaminoacrylate hydrochloride

White solid (0.132 g).

1H-NMR (300 MHz, DMSO-d6) δ (ppm): 9.31 (s, 2H), 9.03 (s, 2H), 4.21 (d, J = 8.8 Hz, 2H), 3.70 (s, 2H), 1.82 - 1.56 (m, 1H), 1.34 - 0.94 (m, 6H).

Intermediate y8: Neopentyl 3,3-diaminoacrylate hydrochloride

White solid (0.791 g).

1H-NMR (300 MHz, DMSO-d6) δ (ppm): 9.25 (s, 2H), 8.97 (s, 2H), 3.79 (s, 2H), 3.66 (s, 2H), 0.89 (s, 9H).

Intermediate y9: 2,2,2-Trifluoroethyl (E)-3-amino-3-(2,2,2-trifluoroethoxy)acrylate

The title compound was prepared following Step 0 of General Procedure y and a slightly modified version of Steps 1 and 2, as outlined below.

Step 1: Into a mixture of 2,2,2-trifluoroethyl 2-cyanoacetate (3.5 g, 20.94 mmol, 1.15 eq) and 2,2,2-trifluoroethyl-1-ol (1.32 mL, 18.21 mmol, 1 eq) that was stirred at - 20°C, HCl gas was bubbled for 60 min. Then, the mixture was left at 4°C for two days without stirring. After that time the mixture was stirred again, and cold ether was added at 0°C. The solid was filtered off and washed with cold ether to afford 2,2,2-trifluoroethyl (E)-3-amino-3-(2,2,2-trifluoroethoxy)acrylate hydrochloride as a white solid (2.5 g, 40%).
1H-NMR (300 MHz, DMSO-d6) δ (ppm): 7.70 (s, 1H), 5.83 (s, 2H), 4.73 (ddq, J = 37.4, 18.4, 9.0 Hz, 4H), 3.89 (s, 1H).

Step 2: To a mixture of 2,2,2-trifluoroethyl (E)-3-amino-3-(2,2,2-trifluoroethoxy) acrylate hydrochloride (0.13 g, 0.43 mmol, 1.0 eq.) and ether (5 ml) was added a solution of potassium carbonate (0.088 g, 0.64 mmol, 1.5 eq) in water (1.5 mL) at 0°C. The resultant mixture was stirred until the solid dissolved. The mixture was extracted with ether, and the organic phase was dried (Na2SO4) and concentrated to afford 2,2,2-trifluoroethyl (E)-3-amino-3-(2,2,2-trifluoroethoxy) acrylate as colorless oil (0.12 g, 98%) which was used as such in the next step.

Intermediate y10: 2-Fluoro-2-methylpropyl 3-amino-3-iminopropanoate hydrochloride

General Procedure y (Steps 0 to 3) afforded the title compound as white solid (0.28 g). 1H-NMR (300 MHz, DMSO-d6) δ (ppm): 9.33 (s, 2H), 9.04 (s, 2H), 4.19 (d, J = 21.1 Hz, 2H), 3.76 (s, 2H), 1.41-1.34 (m, 6H).

Intermediate y11: Cyclopropylmethyl 3,3-diaminoacrylate hydrochloride

General Procedure y (Steps 0 to 3) afforded the title compound as white solid (0.48 g).
1H-NMR (300 MHz, DMSO-d6) δ (ppm): 9.13 (bs, 3H), 8.83 (bs, 2H), 5.13 (t, J = 6.0 Hz, 1H), 3.57 (s, 2H), 1.86 - 1.80 (m, 1H), 1.68 - 1.58 (m, 4H).

Intermediate y12: 4,4-Difluorocyclohexyl 3-amino-3-iminopropanoate hydrochloride

General Procedure y (Steps 0 to 3) afforded title compound as white solid (0.098 g).
1H-NMR (300 MHz, DMSO-d6) δ (ppm): δ 9.32 (s, 2H), 9.02 (s, 2H), 4.96 - 4.66 (m, 1H), 3.41 (s, 2H), 2.10 - 1.76 (m, 8H).

Intermediate y13: 4-Fluorobenzyl 3-amino-3-iminopropanoate hydrochloride

General Procedure y (Steps 0 to 3) afforded title compound as white solid (0.45 g).
1H-NMR (300 MHz, DMSO-d6) δ (ppm): 9.30 (bs, 2H), 9.01 (bs, 2H), 7.49 - 7.43 (m, 2H), 7.26 - 7.17 (m, 2H), 5.17 (s, 2H), 3.73 (s, 2H).

Intermediate y14: Cyclopentylmethyl 3-amino-3-iminopropanoate hydrochloride

General Procedure y (Steps 0 to 3) afforded title compound as white solid (0.22 g).
1H-NMR (300 MHz, DMSO-d6) δ (ppm): 9.31 (bs, 2H), 9.03 (bs, 2H), 3.96 (d, 2H), 3.76 (s, 2H), 2.2 - 2.1 (m, 1H), 1:8 - 1.6 (m, 2H), 1.6 - 1.4 (m, 4H), 1.4 - 1.1 (m, 2H).

PREPARATION OF KNOEVENAGEL INTERMEDIATES General procedure j (adapted from Lu Liu et al, Chem. Int. Ed. 2009, 48, 6093-6096) Intermediate j1: 3-(benzo[b]thiophen-3-ylmethylene)pentane-2,4-dione

Benzo[b]thiophene-3-carbaldehyde (1 g, 6.16 mmol, 1 eq) was dissolved in toluene (0.5 M) at RT, and acetic acid (0.176 ml, 3.08 mmol, 0.5 eq), piperidine (0.061 ml, 0,61 mmol, 0.1 eq), molecular sieves (6 g) and 2,4-pentanedione (0.95 ml, 9.24 mmol, 1.5 eq) were added. The reaction mixture was heated in a microwave oven at 111 °C until the disappearance of starting material (4 h). The mixture was allowed to cool to RT and then concentrated. The resulting residue was purified by flash column chromatography on silica gel using ethyl acetate/hexane (1:6) as eluent to afford the Knoevenagel product as a yellow solid (1.8 g, 60%).
1H-NMR (300 MHz, CDCl3) δ = 7.89 (t, J = 7.5 Hz, 2H), 7.76 (s, 1H), 7.72 (s, 1H), 7.48 (p, J = 7.4 Hz, 2H), 2.49 (s, 3H), 2.30 (s, 3H).

The following intermediates were synthesized according to General procedure j.

Intermediate j2: (E)-2-(benzo[b]thiophen-3-ylmethylene)-1-(pyridin-3-yl)butane-1,3-dione

Yellow solid (0.25 g, 33%).

1H-NMR (300 MHz, CDCl3) δ = 9.03 (s, 1H), 8.72 (d, J = 3.6 Hz, 1H), 8.20 (ddd, J= 8.0, 2.3, 1.7 Hz, 1H), 8.14 (d, J = 0.9 Hz, 1H), 7.96 (dt, J = 8.1, 1.0 Hz, 1H), 7.83 (ddd, J = 7.9, 1.2, 0.7 Hz, 1H), 7.53 (s, 1H), 7.52 - 7.32 (m, 3H), 2.52 (s, 3H).

Intermediate j3: Methyl (Z)-2-(benzo[b]thiophen-3-ylmethylene)-4-chloro-3-oxobutanoate

Yellow solid (0.4 g, 32%).

1H-NMR (300 MHz, CDCl3) δ = 8.16 (s, 1H), 8.03 - 7.86 (m, 2H), 7.82 (s, 1H), 7.60 - 7.39 (m, 2H), 4.34 (s, 2H), 3.90 (s, 3H).

Intermediate j4: (E)-2-(Benzo[b]thiophen-3-ylmethylene)-1-cyclopropylbutane-1,3-dione

Yellow oil (0.4 g, 63%).

1H-NMR (300 MHz, CDCl3) δ = 7.96 - 7.84 (m, 3H), 7.75 (d, J = 1.1 Hz, 1H), 7.56 - 7.38 (m, 2H), 2.45 (s, 3H), 2.03 (dt, J = 7.9, 3.6 Hz, 1H), 1.28 - 1.08 (m, 2H), 1.04 - 0.83 (m, 2H).

Intermediate j5: Tetrahydro-2H-pyran-4-yl (Z)-3-oxo-2-(thieno[2,3-b]pyridin-3-ylmethylene)butanoate

Yellow oil (0.04 g, 25%).

1H-NMR (300 MHz, CDCl3) δ = 8.63 (d, J = 4.6 Hz, 1H), 8.14 (d, J = 8.1 Hz, 1H), 7.94 (s, 1H), 7.76 (s, 1H), 7.40 (dd, J = 8.1, 4.6 Hz, 1H), 5.16 (tt, J = 8.6, 4.1 Hz, 1H), 3.81 (dt, J = 11.8, 4.6 Hz, 2H), 3.49 (ddd, J = 11.8, 8.8, 3.0 Hz, 2H) , 2.46 (s, 3H), 1.91 (dp, J = 8.5, 4.1 Hz, 2H), 1.63 (dtd, J = 13.0, 8.5, 4.1 Hz, 2H).

Intermediate j6: Isopropyl (Z)-3-(7-bromobenzo[b]thiophen-3-yl)-2-(cyclopropanecarbonyl)acrylate

Yellow oil (0.13 g, 32%).

1H-NMR (300 MHz, CDCl3) δ = 7.88 (s, 2H), 7.85 (s, 1H), 7.57 - 7.53 (m, 1H), 7.38 - 7.31 (m, 1H), 5.19 (p, J = 6.3 Hz, 1H), 2.08 (tt, J = 7.8, 4.6 Hz, 1H), 1.35 (s, 3H), 1.32 (s, 3H), 1.21 - 1.11 (m, 2H), 1.02 - 0.91 (m, 2H).

Intermediate j7: Methyl (Z)-2-((7-bromobenzo[b]thiophen-3-yl)methylene)-4,4,4-trifluoro-3-oxobutanoate

Yellow solid (0.040 g, 10%).

1H-NMR (300 MHz, CDCl3) δ = 8.22 (d, J = 1.0 Hz, 1H), 7.87 (t, J = 1.3 Hz, 1H), 7.78 - 7.75 (m, 1H), 7.69 (d, J = 5.0 Hz, 1H), 7.38 - 7.34 (m, 1H), 3.92 (s, 3H). 19F NMR (282 MHz, CDCl3) δ = - 75.69 (s, CF3).

Intermediate j8: Cyclopropylmethyl (Z)-3-(7-bromobenzo[b]thiophen-3-yl)-2-(cyclopropanecarbonyl)acrylate

Yellow solid (0.25 g, 59%).

1H-NMR (300 MHz, CDCl3) δ = 7.96 - 7.84 (m, 3H), 7.58 (d, J = 7.6 Hz, 1H), 7.40 - 7.33 (m, 1H), 4.13 (d, J = 7.5 Hz, 2H), 2.10 (tt, J = 7.5, 4.6 Hz, 1H), 1.25 - 1.18 (m, 3H), 0.99 (dt, J = 7.9, 3.5 Hz, 2H), 0.71 - 0.46 (m, 2H), 0.41 - 0.23 (m, 2H).

Intermediate j9: Cyclopropylmethyl (Z)-3-(7-bromobenzo[b]thiophen-3-yl)-2-(cyclopropanecarbonyl)acrylate

Yellow solid (0.13 g, 50%).

1H-NMR (300 MHz, CDCl3) δ = 8.33 - 8.24 (m, 2H), 7.93 (s, 1H), 7.78 (s, 1H), 7.72 (t, J = 7.8 Hz, 1H), 2.50 (s, 3H), 2.30 (s, 3H). 19F NMR (282 MHz, CDCl3) δ = - 69.81 (s, CF3).

Intermediate j10: 3-((7-Chlorobenzo[b]thiophen-3-yl)methylene)pentane-2,4-dione Yellow solid (0.158 g, 56 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 7.77 (d, J = 7.1 Hz, 2H), 7.69 (s, 1H), 7.45 (d, J = 5.0 Hz, 2H), 2.48 (s, 3H), 2.29 (s, 3H).

Intermediate j11: 3-((7-(Trifluoromethyl)benzo[b]thiophen-3-yl)methylene)pentane-2,4-dione

Yellow solid (0.052 g, 50 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 8.06 (d, J = 8.0 Hz, 1H), 7.82 (s, 1H), 7.80 - 7.73 (m, 2H), 7.61 (t, J = 8.0 Hz, 1H), 2.50 (s, 3H), 2.30 (s, 3H).

19F NMR (282 MHz, CDCl3-d) δ (ppm): -62.87 (CF3).

Intermediate j12: 3-(2-Acetyl-3-oxobut-1-en-1-yl)benzo[b]thiophene-7-carbonitrile Dark-yellow solid (0.132 g, 68 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 8.10 (d, J = 8.1 Hz, 1H), 7.85 (s, 1H), 7.81 (d, J = 7.5 Hz, 1H), 7.69 (s, 1H), 7.60 (td, J = 8.1, 7.4, 1.0 Hz, 1H), 2.49 (s, 3H), 2.31 (s, 3H).

Intermediate j13: Cyclopropylmethyl (Z)-2-(cyclopropanecarbonyl)-3-(thieno[2,3-b]pyridin-3-yl)acrylate

Yellow solid (0.146 g, 61 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 8.63 (d, J = 4.6 Hz, 1H), 8.20 (d, J = 8.4 Hz, 1H), 7.91 (s, 1H), 7.90 (s, 1H), 7.40 (ddd, J = 8.4, 4.6, 0.8 Hz, 1H), 4.12 (d, J = 7.2 Hz, 2H), 2.21 - 2.04 (m, 1H), 1.29 - 1.13 (m, 3H), 1.11 - 0.93 (m, 2H), 0.67 - 0.54 (m, 2H), 0.43 - 0.27 (m, 2H).

Intermediate j14: Cyclopentyl (Z)-2-(cyclopropanecarbonyl)-3-(thieno[2,3-b]pyridin-3-yl)acrylate

Light-yellow solid (0.21 g, 90 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 8.63 (dd, J = 4.6, 1.4 Hz, 1H), 8.19 (dd, J= 8.2, 1.4 Hz, 1H), 7.89 (s, 1H), 7.86 (s, 1H), 7.40 (dd, J = 8.2, 4.6 Hz, 1H), 5.36 (tt, J = 5.7, 2.5 Hz, 1H), 2.10 (tt, J = 8.0, 4.6 Hz, 1H), 1.98 - 1.86 (m, 2H), 1.86 - 1.70 (m, 4H), 1.69 - 1.57 (m, 2H), 1.19 (dt, J = 6.9, 3.5 Hz, 2H), 0.99 (dt, J = 8.0, 3.5 Hz, 2H).

Intermediate j15: Cyclopropylmethyl (Z)-3-(7-cyanobenzo[b]thiophen-3-yl)-2-(cyclopropanecarbonyl)acrylate

Yellow solid (0.23 g, 69 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 8.19 - 8.09 (m, 1H), 7.95 (s, 1H), 7.92 (s, 1H), 7.81 - 7.74 (m, 1H), 7.62 - 7.53 (m, 1H), 4.13 (d, J = 7.2 Hz, 2H), 2.12 (tt, J = 7.8, 4.6 Hz, 1H), 1.29 - 1.13 (m, 3H), 1.07 - 0.93 (m, 2H), 0.67 - 0.55 (m, 2H), 0.35 (dt, J = 6.2, 4.6 Hz, 2H).

Intermediate j16: Methyl (Z)-3-(7-cyanobenzo[b]thiophen-3-yl)-2-(cyclopropanecarbonyl)acrylate

Yellow solid (0.21 g, 80 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 8.13 (t, J = 9.1 Hz, 1H), 8.02 - 7.86 (m, 2H), 7.79 (d, J = 7.7 Hz, 1H), 7.57 (td, J = 7.7, 2.7 Hz, 1H), 3.89 (s, 3H), 2.13 - 2.01 (m, 1H), 1.28 - 1.16 (m, 2H), 1.14 - 0.93 (m, 2H).

Intermediate j17: Methyl (Z)-2-((7-cyanobenzo[b]thiophen-3-yl)methylene)-3-oxobutanoate

Yellow solid (0.11 g, 60 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 8.11 (d, J = 8.2 Hz, 1H), 7.99 (s, 1H), 7.84 (s, 1H), 7.80 (d, J = 7.4 Hz, 1H), 7.62 - 7.54 (m, 1H), 3.87 (s, 3H), 2.47 (s, 3H).

Intermediate j18: (E)-2-((7-cyanobenzo[b]thiophen-3-yl)methylene)-3-oxobutanamide Yellow solid (0.17 g, 58 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 8.35 (s, 1H), 8.14 - 8.07 (m, 1H), 7.88 - 7.73 (m, 2H), 7.63 - 7.54 (m, 1H), 6.08 (bs, 1H), 5.87 (bs, 1H), 2.55 (s, 3H).

Intermediate j19: Cyclopropyl (E)-2-((7-cyanobenzo[b]thiophen-3-yl)methylene)-3-oxobutanoate

Yellow solid (0.104 g, 30 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 8.09 (d, J = 8.2 Hz, 1H), 8.02 (s, 1H), 7.80 (d, J = 7.3 Hz, 2H), 7.63 - 7.51 (m, 1H), 4.43 - 4.31 (m, 1H), 2.46 (s, 3H), 0.85 - 0.74 (m, 2H), 0.73 - 0.61 (m, 2H).

Intermediate j20: Methyl (E)-2-((7-cyano-5-fluorobenzo[b]thiophen-3-yl)methylene)-3-oxobutanoate

Yellow solid (0.136 g, 60 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 8.07 (s, 1H), 7.79 (d, J = 8.7 Hz, 1H), 7.72 (s, 1H), 7.56 (d, J = 7.7 Hz, 1H), 3.87 (s, 3H), 2.47 (s, 3H).

Intermediate j21: Methyl (E)-2-((7-cyano-4-fluorobenzo[b]thiophen-3-yl)methylene)-3-oxobutanoate

Yellow solid (0.116 g, 63 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 8.11 (s, 1H), 7.85 (s, 1H), 7.76 (dd, J = 8.2, 4.3 Hz, 1H), 7.24 - 7.16 (m, 1H), 3.89 (s, 3H), 2.35 (s, 3H).

Intermediate j22: Methyl (E)-2-((7-cyanothieno[3,2-b]pyridin-3-yl)methylene)-3-oxobutanoate

Yellow solid (0.77 g, 40 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 8.91 (d, J = 4.7 Hz, 1H), 8.32 (s, 1H), 8.15 (s, 1H), 7.63 (d, J = 4.7 Hz, 1H), 3.90 (s, 3H), 2.53 (s, 3H).

Intermediate j23: Cyclopropylmethyl (E)-2-((7-cyanobenzo[b]thiophen-3-yl)methylene)-3-oxobutanoate

Yellow solid (0.45 g, 52 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 8.11 (dd, J = 8.2, 0.9 Hz, 1H), 8.06 (s, 1H), 7.83 (d, J = 0.9 Hz, 1H), 7.79 (d, J = 7.5 Hz, 1H), 7.57 (dd, J = 8.2, 7.5 Hz, 1H), 4.13 (d, J = 7.4 Hz, 2H), 2.48 (s, 3H), 1.26 - 1.04 (m, 1H), 0.66 - 0.51 (m, 2H), 0.38 - 0.21 (m, 2H).

Intermediate j24: Prop-2-yn-1-yl (E)-2-((7-cyanobenzo[b]thiophen-3-yl)methylene)-3-oxobutanoate

Yellow solid (0.7 g, 85 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 8.13 (dd, J = 8.2, 0.9 Hz, 1H), 7.99 (s, 1H), 7.96 (s, 1H), 7.80 (d, J = 7.1 Hz, 1H), 7.62 - 7.55 (m, 1H), 4.89 (d, J = 2.5 Hz, 2H), 2.55 (t, J = 2.5 Hz, 1H), 2.40 (s, 3H).

Intermediate j25: Methyl (E)-2-((5,7-dicyanobenzo[b]thiophen-3-yl)methylene)-3-oxobutanoate

Yellow solid (0.135 g, 59 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 8.31 (d, J = 1.4 Hz, 1H), 7.96 - 7.87 (m, 2H), 7.49 (s, 1H), 4.13 (d, J = 1.0 Hz, 3H), 2.04 (d, J = 1.0 Hz, 3H).

Intermediate j26: But-2-yn-1-yl (E)-2-((7-cyanobenzo[b]thiophen-3-yl) methylene)-3-oxobutanoate

Yellow solid (0.125 g, 36 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 8.11 (td, J = 4.4, 0.8 Hz, 2H), 7.86 (d, J = 0.8 Hz, 1H), 7.80 (d, J = 7.4 Hz, 1H), 7.58 (dd, J = 8.2, 7.4 Hz, 1H), 4.86 (q, J = 2.3 Hz, 2H), 2.48 (s, 3H), 1.89 (t, J = 2.3 Hz, 3H).

Intermediate j27: (2,2,2-Trifluoroethyl) -2-((7-cyanobenzo[b]thiophen-3-yl) methylene)-3-oxobutanoate

Yellow solid (0.079 g, 38 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 8.15 - 7.98 (m, 2H), 7.95 - 7.88 (m, 1H), 7.86 - 7.72 (m, 2H), 4.66 (dq, J = 11.7, 8.3 Hz, 2H), 2.53 - 2.40 (m, 3H).

Intermediate j28: Methyl (E)-2-((6-chloro-7-cyanobenzo[b]thiophen-3-yl) methylene)-3-oxobutanoate

Yellow solid (0.24 g, 56 %).

1H-NMR (300 MHz, CDCl3-d) δ (ppm): 8.01 (d, J = 8.7 Hz, 1H), 7.91 (d, J = 0.9 Hz, 1H), 7.84 (d, J = 0.9 Hz, 1H), 7.58 (d, J = 8.7 Hz, 1H), 3.90 (s, 3H), 2.36 (s, 3H).

Intermediate j29: (E)-3-(2-Cyano-3-oxobut-1-en-1-yl)benzo[b]thiophene-7-carbonitrile

General procedure j using 3-oxobutanenitrile gave title compound as yellow solid (0.23 g, 69 %).

1H-NMR (300 MHz, DMSO-d6) δ (ppm): 9.07 (s, 1H), 8.47 (s, 1H), 8.22 (d, J = 8.2 Hz, 1H), 7.85 (d, J = 7.4 Hz, 1H), 7.64 (t, J = 7.8 Hz, 1H), 2.63 (s, 3H).

Intermediate j30: 3-((5-Fluorothieno[2,3-b]pyridin-3-yl)methylene)pentane-2,4-dione

General procedure j gave title compound as yellow solid (0.132 g, 61 %).
1H-NMR (300 MHz, DMSO-d6) δ (ppm): 8.56 (d, J = 2.4 Hz, 1H), 7.90 (s, 1H), 7.83 (dd, J = 8.7, 2.4 Hz, 1H), 7.55 (s, 1H), 2.48 (s, 3H), 2.32 (s, 3H).

EXAMPLES PREPARATION OF DIHYDROPYRIDINES Method A (Rampa, A et al, Forsch. 1992, 42, 1284). Example A1: Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

A mixture of 3-(benzo[b]thiophen-3-ylmethylene)pentane-2,4-dione (0.24 g, 0.98 mmol, 1 eq), methyl 3-oxobutanoate (0.158 ml, 1.47 mmol, 1.5 eq) and 30% aqueous ammonium (0.62 ml, 9.8 mmol, 10 eq) in i-PrOH (2 ml) was heated at reflux teperature for 12 h. The solvent was removed under reduced pressure. The residue was taken up in water and extracted with dichloromethane and ethyl acetate. The combined organic layers were washed with brine, dried (Na2SO4), filtered and concentrated, and the crude product was purified by flash column chromatography on silica gel using CH2Cl2/MeOH (1%) as eluent to afford the title compound as a yellow solid (114 mg, 25%).
1H-NMR (300 MHz, CDCl3) δ = 8.10 (d, J = 8.0 Hz, 1H), 7.79 (d, J = 7.9 Hz, 1H), 7.34 (dt, J = 24.0, 7.1 Hz, 2H), 7.13 (s, 1H), 6.27 (bs, 1H), 5.47 (s, 1H), 3.64 (s, 3H), 2.34 (s, 3H), 2.27 (s, 3H), 2.14 (s, 3H).

HRMS (IE) m/z calculated C19H19NO3S [M+]: 341.1086, found: 341.1069.

Example A1 is a racemic mixture of two stereoisomers that have been separated by semi-preparative chiral HPLC (column: ChiralPak IA, 5 µm, 4.6 x 250 mm, eluent: n- hexane/iPrOH 95:5) affording Example A2 (Enantiomer 1, Tr= 22,54 min, 95,60 % ee; 96,46% purity) and Example A3 (Enantiomer 2, Tr= 18,10 min, 99,88 % ee; 92,87 % purity). Purity has been determined on a RP-C18 analytical column (Gemini-NX C18 5 µm, 4,6 x 150 mm; eluents: ACN/water/100 mM ammonium acetate, pH 7). The absolute stereochemistry has not been determined.

Example A4: 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxamide

Method A yielded the title compound as a yellow solid (73 mg, 22%).
1H-NMR (300 MHz, CDCl3) δ = 8.05 (d, J = 7.7 Hz, 1H), 7.76 (d, J = 7.6 Hz, 1H), 7.29 (dt, J = 15.0, 7.0 Hz, 2H), 7.09 (s, 1H), 6.76 (s, 1H), 5.65 (bs, 2H), 5.34 (s, 1H), 2.25 (s, 3H), 2.08 (s, 6H).
HRMS (IE) m/z calculated C18H18N2O2S: 326.0910, found: 326.1011.

Example A5: 1-(4-(benzo[b]thiophen-3-yl)-5-benzoyl-2,6-dimethyl-1,4-dihydropyridin-3-yl)ethan-1-one

Method A yielded the title compound as a dark yellow solid (63 mg, 16%). 1H-NMR (300 MHz, CDCl3) δ = 7.79 - 7.70 (m, 1H), 7.65 - 7.56 (m, 1H), 7.53 - 7.31 (m, 5H), 7.25 - 7.18 (m, 2H), 7.06 (s, 1H), 5.72 (bs, 1H), 5.56 (s, 1H), 2.48 (s, 3H), 2.07 (s, 3H), 1.77 (s, 3H).
HRMS (IE): m/z calculated C24H21NO2S [M+]: 387.1293, found: 387.1286.

Example A6: Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-methyl-2-phenyl-1,4-dihydropyridine-3-carboxylate

Method A in MeOH yielded the title compound as a dark yellow solid (15 mg, 5%).
1H-NMR (300 MHz, CDCl3) δ = 8.21 (d, J = 8.2 Hz, 1H), 7.83 (d, J= 7.9 Hz, 1H), 7.45-7.31 (m, 5H), 7.30 - 7.24 (m, 3H), 6.02 (bs, 1H), 5.59 (s, 1H), 3.38 (s, 3H), 2.41 (s, 3H), 2.17 (s, 3H).
HRMS (IE): m/z calculated C24H21NO3S [M+]: 403.1242, found: 403.1252.

In the Hantzsch synthesis of the following examples, preformed enamines have been used instead of mixtures of 1,3-dicarbonyl compounds and ammonia:

Example A8: 1-(4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-5-nicotinoyl-1,4-dihydro pyridin-3-yl)ethan-1-one

Method A using (E)-4-aminopent-3-en-2-one in EtOH yielded the title compound as a dark yellow solid (60 mg, 19%).
1H-NMR (300 MHz, CDCl3) δ = 8.64 (d, J = 11.7 Hz, 2H), 7.84 - 7.60 (m, 3H), 7.35 - 7.21 (m, 3H), 7.08 (s, 1H), 5.97 (bs, 1H), 5.56 (s, 1H), 2.45 (s, 3H), 2.10 (s, 3H), 1.77 (s, 3H).
HRMS (IE): m/z calculated C23H20N2O2S [M+]: 388.1245, found: 388.1229.

Example A9: Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydro-[2,3'-bipyridine]-3-carboxylate

Method A using methyl (Z)-3-amino-3-(pyridin-3-yl)acrylate in acetic acid yielded the title compound as a yellow solid (31 mg, 16%).
1H-NMR (300 MHz, CDCl3) δ = 8.49 (s, 1H), 8.42 (s, 1H), 8.18 (d, J = 8.0 Hz, 1H), 7.83 (d, J = 8.0 Hz, 1H), 7.55 (d, J = 7.9 Hz, 1H), 7.41 (t, J = 7.5 Hz, 1H), 7.33 (t, J = 7.2 Hz, 1H), 7.26 - 7.16 (m, 2H), 6.68 (bs, 1H), 5.59 (s, 1H), 3.37 (s, 3H), 2.41 (s, 3H), 2.15 (s, 3H).
HRMS (IE) m/z calculated C23H20N2O3S [M+]: 404.1195, found: 404.1179.

Example A10: 2,2,2-trifluoroethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method A using 2,2,2-trifluoroethyl (E)-3-aminobut-2-enoate in 2,2,2-trifluoroethanol yielded the title compound as a yellow solid (190 mg, 58%).
1H-NMR (300 MHz, CDCl3) δ = 8.08 (d, J = 7.9 Hz, 1H), 7.81 (d, J = 8.0 Hz, 1H), 7.35 (dt, J = 21.4, 7.1 Hz, 2H), 7.17 (s, 1H), 6.10 (bs, 1H), 5.47 (s, 1H), 4.58 - 4.27 (m, 2H), 2.37 (s, 3H), 2.31 (s, 3H), 2.16 (s, 2H). 19F-NMR (300 MHz, CDCl3) δ = -73.39 (t, J = 8.1 Hz).
HRMS (IE) m/z calculated C20H18F3NO3S [M+]: 409.0960, found: 409.0943.

Example A10 was hydrolyzed to afford the carboxylic acid Example A11: Example A11: 5-Acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylic acid

2,2,2-trifluoroethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate (0.05 g, 0.14 mmol, 1 eq.) was dissolved in dioxane (3 ml) at room temperature. Sodium hydroxide (0.42 ml, 0.84 mmol, 6 eq) was added, and the reaction mixture was heated at 130 °C for 4 hours. The solvent was removed under reduced pressure, and water and 2N HCl was added (pH 6-7). The mixture was extracted with dichloromethane and ethyl acetate, and the combined organic layers were washed with brine, dried (Na2SO4), filtered and concentrated. The crude product was purified by flash column chromatography on silica gel using CH2Cl2/MeOH (4%) as eluent to afford the title compound (15 mg, 38%).
1H-NMR (300 MHz, CDCl3) δ = 11.82 (s, 1H), 8.95 (s, 1H), 8.14 (dd, J = 6.9, 1.3 Hz, 1H), 7.89 (dd, J = 7.0, 1.6 Hz, 1H), 7.32 (td, J = 7.6, 1.5 Hz, 2H), 7.18 (s, 1H), 5.40 (s, 1H), 2.32 (s, 3H), 2.24 (s, 3H), 2.10 (s, 3H).
HRMS (IE) m/z calculated C18H17NO3S [M+]: 327.0929, found: 327.0937.

Method B (adapted from WO2006047537) Example B1: 1-(4-(Benzo[b]thiophen-3-yl)-2,6-dimethyl-5-(4-methylpiperazine-1-carbonyl)-1,4-dihydropyridin-3-yl)ethan-1-one

A mixture of 3-(benzo[b]thiophen-3-ylmethylene)pentane-2,4-dione (0.12 g, 0.49 mmol, 1 eq), 1-(4-methylpiperazin-1-yl)butane-1,3-dione (0.1 g, 0.54 mmol, 1.1 eq), ammonium acetate (0.06 g, 0.73 mmol, 1.5 eq) in MeOH (2 ml) was heated in a microwave reactor at 130°C for 15 min. The reaction mixture was allowed to cool to RT and then concentrated. The residue was purified by flash column chromatography on silica gel using CH2Cl2/MeOH (5%) as eluent to afford the title compound as a yellow solid (30 mg, 16%).
1H-NMR (300 MHz, CDCl3) δ = 7.87 (d, J = 7.6 Hz, 1H), 7.80 (d, J = 7.3 Hz, 1H), 7.34 (dt, J = 14.8, 6.7 Hz, 2H), 7.09 (s, 1H), 5.91 (bs, 1H), 5.38 (s, 1H), 3.90 (d, J = 11.6 Hz, 1H), 3.11 - 2.92 (m, 1H), 2.91 - 2.73 (m, 1H), 2.68 - 2.55 (m, 1H), 2.37 (s, 3H), 2.33 - 2.19 (m, 2H), 1.90 (s, 3H), 1.85 (s, 3H), 1.69 (s, 3H), 1.55 - 1.36 (m, 1H), 0.40 - 0.17 (m, 1H).
HRMS (IE): m/z calculated C23H27N3O2S [M+]: 409.1824, found: 409.1834.

Example B2: 5-Acetyl-4-(benzo[b]thiophen-3-yl)-N,N-diethyl-2,6-dimethyl-1,4-dihydropyridine-3-carboxamide

Method B yielded the title compound as a yellow solid (30 mg, 16%).
1H-NMR (300 MHz, CDCl3) δ = 7.86 (d, J = 7.3 Hz, 1H), 7.78 (d, J = 7.8 Hz, 1H), 7.35-7.28 (m, 2H), 7.11 (s, 1H), 5.68 (bs, 1H), 5.37 (s, 1H), 3.50 - 3.28 (m, 1H), 3.23 - 2.94 (m, 1H), 2.86 - 2.75 (m, 1H), 2.70 - 2.45 (m, 1H), 2.37 (s, 3H), 1.93 (s, 3H), 1.71 (s, 3H), 0.94 (t, J = 6.0 Hz, 3H), 0.20 (s, 3H).
HRMS (IE) m/z calculated C22H26N2O2S [M+]: 382.1715, found: 382.1724.

Example B3: 1-(4-(Benzo[b]thiophen-3-yl)-2,6-dimethyl-5-(morpholine-4-carbonyl)-1,4-dihydropyridin-3-yl)ethan-1-one

Method B yielded the title compound as a yellow solid (18 mg, 12%).
1H-NMR (300 MHz, CDCl3) δ = 7.89 (d, J = 7.3 Hz, 1H), 7.83 (d, J = 7.9 Hz, 1H), 7.45-7.28 (m, 2H), 7.11 (s, 1H), 5.66 (bs, 1H), 5.39 (s, 1H), 3.84 (d, J = 11.8 Hz, 1H), 3.54 - 3.36 (m, 2H), 3.14 - 2.97 (m, 2H), 2.90 - 2.79 (m, 2H), 2.66 - 2.45 (m, 1H), 2.38 (s, 3H), 1.93 (s, 3H), 1.70 (s, 3H).
HRMS (IE): m/z calculated C22H24N2O3S [M+]: 396.1508, found: 396.1494.

Example B4: 2-Methoxyethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method B yielded the title compound as a yellow solid (30 mg, 16%).
1H-NMR (300 MHz, CDCl3) δ = 8.13 (d, J = 8.0 Hz, 1H), 7.80 (d, J = 7.9 Hz, 1H), 7.45-7.29 (m, 2H), 7.17 (s, 1H), 5.90 (bs, 1H), 5.49 (s, 1H), 4.20 (t, J = 4.8 Hz, 2H), 3.57 - 3.50 (m, 2H), 3.33 (s, 3H), 2.37 (s, 3H), 2.30 (s, 3H), 2.14 (s, 3H).
HRMS (IE): m/z calculated C21H23NO4S [M+]: 385.1348, found: 385.1335.

Example B5: 3-Acetamidopropyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method B yielded the title compound as a yellow solid (22 mg, 11%).
1H-NMR (300 MHz, CDCl3) δ = 8.14 (d, J = 8.0 Hz, 1H), 7.79 (d, J = 8.0 Hz, 1H), 7.38 (t, J = 7.6 Hz, 1H), 7.34 - 7.25 (m, 1H), 7.14 (s, 1H), 5.56 (bs, 1H), 5.46 (s, 1H), 4.24 - 3.95 (m, 2H), 3.06 - 2.71 (m, 2H), 2.32 (s, 3H), 2.30 (s, 3H), 2.16 (s, 3H), 2.04 - 1.94 (m, 1H), 1.85 (s, 3H), 1.81 - 1.43 (m, 2H).
HRMS (IE): m/z calculated C23H26N2O4S [M+]: 426.1613, found: 426.1603.

Example B6: Benzyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydro-pyridine-3-carboxylate

Method B yielded the title compound as a yellow solid (19 mg, 10%).
1H-NMR (300 MHz, CDCl3) δ = 8.01 (d, J = 8.7 Hz, 1H), 7.79 (d, J = 8.5 Hz, 1H), 7.41 - 7.26 (m, 5H), 7.26 - 7.19 (m, 2H), 7.09 (s, 1H), 5.87 (bs, 1H), 5.49 (s, 1H), 5.19 - 5.01 (m, 2H), 2.36 (s, 3H), 2.30 (s, 3H), 2.12 (s, 3H).
HRMS (IE): m/z calculated C25H23NO3S [M+]: 417.1399, found: 417.1399.

Example B7: 2-Morpholinoethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method B yielded the title compound as a yellow solid (30 mg, 11%).
1H-NMR (300 MHz, CDCl3) δ = 8.11 (d, J= 9.1 Hz, 1H), 7.79 (d, J= 7.1 Hz, 1H), 7.42 - 7.25 (m, 2H), 7.15 (s, 1H), 6.07 (bs, 1H), 5.49 (s, 1H), 4.29 - 4.07 (m, 2H), 3.59 (t, J = 4.6 Hz, 4H), 2.51 (t, J = 5.7 Hz, 2H), 2.38 - 2.33 (m, 7H), 2.31 (s, 3H), 2.16 (s, 3H).
HRMS (IE) m/z calculated C24H28N2O4S [M+]: 440.1770, found: 440.1783.

Example B8: 2-(Dimethylamino)ethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method B yielded the title compound as a yellow solid (15 mg, 7%).
1H-NMR (300 MHz, CDCl3) δ = 8.12 (d, J = 8.0 Hz, 1H), 7.79 (d, J = 7.9 Hz, 1H), 7.43-7.23 (m, 2H), 7.15 (s, 1H), 5.97 (bs, 1H), 5.48 (s, 1H), 4.19 (h, J = 5.8 Hz, 2H), 2.59 (q, J = 6.2 Hz, 2H), 2.36 (s, 3H), 2.30 (s, 3H), 2.23 (s, 6H), 2.15 (s, 3H).
HRMS (IE): m/z calculated C22H26N2O3S [M+]: 398.1664, found: 398.1663.

Example B9: 2-Acetamidoethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method B yielded the title compound as a yellow solid (34 mg, 14%).
1H-NMR (300 MHz, CDCl3) δ = 8.19 (d, J = 8.0 Hz, 1H), 7.82 (d, J = 7.9 Hz, 1H), 7.43 (t, J = 7.4 Hz, 1H), 7.34 (t, J = 7.4 Hz, 1H), 7.20 (s, 1H), 5.84 (bs, 1H), 5.47 (s, 1H), 4.86 (bs, 1H), 4.25 - 4.09 (m, 1H), 4.07 - 3.86 (m, 1H), 3.60 - 3.38 (m, 1H), 3.24 - 3.08 (m, 1H), 2.37 (s, 3H), 2.34 (s, 3H), 2.20 (s, 3H), 1.61 (s, 3H).
HRMS (IE): m/z calculated C22H24N2O4S [M+]: 412.1457, found: 412.1458.

Example B10: Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-(methoxymethyl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method B yielded the title compound as a light-yellow solid (25 mg, 14%). 1H-NMR (300 MHz, CDCl3) δ = 8.10 (d, J = 8.0 Hz, 1H), 7.80 (d, J = 7.9 Hz, 1H), 7.42 - 7.27 (m, 3H), 7.15 (s, 1H), 5.46 (s, 1H), 4.59 (dd, J = 26.8 Hz, 2H), 3.63 (s, 3H), 3.47 (s, 3H), 2.41 (s, 3H), 2.14 (s, 3H).
HRMS (IE): m/z calculated C20H21NO4S [M+]: 371.1191, found: 371.1176.

Example B11: Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-((dimethylamino)methyl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method B yielded the title compound as a yellow solid (25 mg, 11%).
1H-NMR (300 MHz, CDCl3) δ = 8.18 (s, 1H), 8.10 (d, J = 8.2 Hz, 1H), 7.80 (d, J = 7.6 Hz, 1H), 7.43 - 7.28 (m, 2H), 7.14 (s, 1H), 5.47 (s, 1H), 3.73 - 3.50 (m, 5H), 2.42 (s, 3H), 2.29 (s, 6H), 2.14 (s, 3H).
HRMS (IE) m/z calculated C21H24N2O3S [M+]: 384.1508, found: 384.1506.

Example B12: Pyridin-4-ylmethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method B yielded the title compound as a yellow solid (25 mg, 13%).
1H-NMR (300 MHz, CDCl3) δ = 8.49 - 8.34 (m, 2H), 8.03 (d, J = 6.8 Hz, 1H), 7.80 (d, J = 8.5 Hz, 1H), 7.35 - 7.20 (m, 2H), 7.14 (s, 1H), 6.93 (s, 1H), 6.91 (s, 1H), 6.33 (s, 1H), 5.52 (s, 1H), 5.08 (q, J = 13.7 Hz, 2H), 2.35 (s, 3H), 2.33 (s, 3H), 2.16 (s, 3H).
HRMS (IE): m/z calculated C24H22N2O3S [M+]: 418.1351, found: 418.1360.

Example B13: 4-Methoxybenzyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method B yielded the title compound as a yellow solid (28 mg, 13%).
1H-NMR (300 MHz, CDCl3) δ = 8.00 (d, J = 8.6 Hz, 1H), 7.78 (d, J = 8.5 Hz, 1H), 7.32 - 7.20 (m, 2H), 7.16 (d, J = 8.5 Hz, 2H), 7.08 (s, 1H), 6.83 (d, J = 8.6 Hz, 2H), 5.93 (s, 1H), 5.47 (s, 1H), 5.02 (d, J = 2.8 Hz, 2H), 3.81 (s, 3H), 2.35 (s, 3H), 2.28 (s, 3H), 2.11 (s, 3H).
HRMS (IE): m/z calculated C26H25NO4S [M+]: 447.1504, found: 447.1506.

Example B15: Pyridin-2-ylmethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method B yielded the title compound as a yellow solid (35 mg, 14%).
1H-NMR (300 MHz, CDCl3) δ = 8.53 (d, J = 4.0 Hz, 1H), 8.04 (d, J = 8.5 Hz, 1H), 7.78 (d, J = 8.5 Hz, 1H), 7.52 - 7.43 (m, 1H), 7.31 - 7.21 (m, 2H), 7.15 (d, J = 5.4 Hz, 2H), 6.91 (d, J = 7.8 Hz, 1H), 6.22 (s, 1H), 5.55 (s, 1H), 5.22 (d, J = 3.8 Hz, 2H), 2.34 (s, 3H), 2.31 (s, 3H), 2.14 (s, 3H).
HRMS (IQ) m/z calculated C24H23N2O3S [M+1]: 419.1429, found: 419.1436.

Example B16: Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-methyl-2-(morpholinomethyl)-1,4-dihydropyridine-3-carboxylate

Method B yielded the title compound as a yellow solid (25 mg, 10%).
1H-NMR (300 MHz, CDCl3) δ = 8.16 - 7.97 (m, 2H), 7.80 (d, J = 8.0 Hz, 1H), 7.42 - 7.17 (m, 2H), 7.11 (s, 1H), 5.47 (s, 1H), 3.82 - 3.69 (m, 5H), 3.64 (s, 4H), 2.52 (t, J = 4.6 Hz, 4H), 2.42 (s, 3H), 2.13 (s, 3H).
HRMS (IE) m/z calculated C23H26N2O4S [M+]: 426.1613, found: 426.1604.

Example B16-a: Dimethyl 4-(benzo[b]thiophen-3-yl)-2,6-bis(morpholinomethyl)-1,4-dihydropyridine-3,5-dicarboxylate

1H-NMR (300 MHz, CDCl3) δ = 9.52 (s, 1H), 8.06 (d, J = 8.4 Hz, 1H), 7.79 (d, J = 7.8 Hz, 1H), 7.47 - 7.14 (m, 2H), 7.05 (s, 1H), 5.48 (s, 1H), 3.80 (d, J = 4.6 Hz, 12H), 3.55 (d, J = 1.7 Hz, 6H), 2.56 (tq, J = 11.7, 5.9, 4.9 Hz, 8H).

HRMS (IE) m/z calculated C27H33N3O6S [M+]: 527.2090, found: 527.2064.

Example B17: 2-Hydroxyethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method B yielded the title compound as a yellow solid (25 mg, 10%).
1H-NMR (300 MHz, CDCl3) δ = 8.14 (d, J = 8.1 Hz, 1H), 7.81 (d, J = 7.9 Hz, 1H), 7.35 (dt, J = 25.8, 7.3 Hz, 2H), 7.17 (s, 1H), 6.07 (s, 1H), 5.48 (s, 1H), 4.25 - 4.05 (m, 2H), 3.68 (s, 2H), 2.34 (s, 3H), 2.32 (s, 3H), 2.16 (s, 3H), 1.83 (bs, 1H).
HRMS (IE): m/z calculated C20H21NO4S [M+]: 371.1191, found: 371.1190.

Example B18: 1-(4-(Benzo[b]thiophen-3-yl)-5-(cyclopropanecarbonyl)-6-cyclopropyl-2-methyl-1,4-dihydropyridin-3-yl)ethan-1-one

Method B yielded the title compound as a yellow solid (15 mg, 10%).
1H-NMR (300 MHz, CDCl3) δ = 8.07 (d, J = 8.0 Hz, 1H), 7.78 (d, J = 8.0 Hz, 1H), 7.45-7.26 (m, 2H), 7.04 (s, 1H), 5.78 (s, 1H), 5.57 (s, 1H), 2.39 (s, 3H), 2.37 - 2.23 (m, 1H), 2.13 (s, 4H), 1.27 (s, 1H), 1.11 (tdd, J = 5.6, 4.3, 2.7 Hz, 1H), 1.05 - 0.88 (m, 3H), 0.86 - 0.75 (m, 1H), 0.70 (d, J = 5.5 Hz, 2H).
HRMS (IE) m/z calculated C23H23NO2S [M+]: 377.1424, found: 377.1428.

Example B19: Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-(1-(tert-butoxycarbonyl) azetidin-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method B yielded the title compound as a light-yellow solid (0.065 mg, 19%).
1H-NMR (300 MHz, CDCl3) δ = 8.06 (d, J = 8.1 Hz, 1H), 7.80 (d, J = 7.8 Hz, 1H), 7.50-7.22 (m, 2H), 7.14 (s, 1H), 6.69 (s, 1H), 5.48 (s, 1H), 4.68 (tt, J = 8.8, 4.6 Hz, 1H), 4.22 (td, J = 8.9, 3.6 Hz, 2H), 3.82 (ddd, J = 14.7, 9.0, 5.0 Hz, 2H), 3.63 (s, 3H), 2.45 (s, 3H), 2.14 (s, 3H), 1.46 (s, 9H).
HRMS (IE): m/z calculated C26H30N2O5S [M+]: 482.1875, found: 482.1874.

Example B20: (1-(tert-Butoxycarbonyl)piperidin-4-yl)methyl 5-acetyl-4-(benzo[b] thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method B yielded the title compound as a yellow solid (0.06 mg, 26%).
1H-NMR (300 MHz, CDCl3) δ = 8.12 (d, J = 7.9 Hz, 1H), 7.78 (d, J = 7.9 Hz, 1H), 7.32 (ddd, J = 23.7, 11.3, 4.4 Hz, 2H), 7.12 (s, 1H), 6.23 (s, 1H), 5.47 (s, 1H), 4.11 - 3.79 (m, 4H), 2.72 - 2.44 (m, 2H), 2.34 (s, 3H), 2.30 (s, 3H), 2.17 (s, 3H), 1.71 - 1.58 (m, 1H), 1.44 (s, 10H), 1.38 - 1.21 (m, 1H), 0.99 (td, J = 11.8, 4.2 Hz, 2H).
HRMS (IE) m/z calculated C29H36N2O5S [M+]: 524.2345, found: 524.2349.

Example B21: Cyclohexylmethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method B yielded the title compound as a light-yellow solid (35 mg, 17%).
1H-NMR (300 MHz, CDCl3) δ = 8.13 (d, J= 7.9 Hz, 1H), 7.79 (d, J= 8.0 Hz, 1H), 7.43-7.19 (m, 2H), 7.12 (s, 1H), 5.96 (s, 1H), 5.49 (s, 1H), 3.87 (d, J = 6.2 Hz, 2H), 2.36 (s, 3H), 2.30 (s, 3H), 2.16 (s, 3H), 1.70 - 1.46 (m, 6H), 1.27 - 1.01 (m, 4H), 1.01 - 0.75 (m, 1H).
HRMS (IE) m/z calculated C25H29NO3S [M+]: 423.1868, found: 423.1876.

Example B22: Methyl 4-(benzo[b]thiophen-3-yl)-5-(cyclopropanecarbonyl)-2,6-dicyclopropyl-1,4-dihydropyridine-3-carboxylate

Method B yielded the title compound as a light-yellow solid (0.030 g, 11 %).
1H-NMR (300 MHz, CDCl3) δ = 8.09 (ddd, J = 8.1, 1.1, 0.7 Hz, 1H), 7.78 (ddd, J = 7.8, 1.1, 0.7 Hz, 1H), 7.45 - 7.27 (m, 2H), 7.03 (s, 1H), 5.66 (bs, 1H), 5.59 (s, 1H), 3.64 (s, 3H), 2.73 (tt, J = 8.5, 5.5 Hz, 1H), 2.37 (tt, J = 8.5, 5.5 Hz, 1H), 2.22 (tt, J = 7.8, 4.6 Hz, 1H), 1.13 - 0.86 (m, 6H), 0.80 - 0.52 (m, 6H).
HRMS (IE) m/z calculated C25H25NO3S [M+]: 419.1555, found: 419.1543.
HPLC (98.4%): Rt 11.68 min.

Example B23: 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-N-phenyl-1,4-dihydropyridine-3-carboxamide

Method B yielded the title compound as a yellow solid (0.04 g, 23 %).
1H-NMR (300 MHz, CDCl3) δ = 8.11 - 8.04 (m, 1H), 7.89 - 7.81 (m, 1H), 7.41 - 7.27 (m, 3H), 7.25 - 6.98 (m, 6H), 5.66 (bs, 1H), 5.44 (s, 1H), 2.38 (s, 3H), 2.22 (s, 3H), 2.16 (s, 3H).
HRMS (IE) m/z calculated C24H22N2O2S [M+]: 402.1402, found: 402.1403.
HPLC (96.5%): Rt 24.12 min.

Method C Example C1: Tetrahydro-2H-pyran-4-yl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

A mixture of tetrahydro-2H-pyran-4-yl (Z)-2-(benzo[b]thiophen-3-ylmethylene)-3-oxobutanoate (0.056 g, 0.17 mmol) and (E)-4-aminopent-3-en-2-one (0.017 g, 0.17 mmol) in methanol (1 mL) was heated in a microwave reactor at 130°C for 60 min. The mixture was allowed to cool to RT and the solvent was removed under reduced pressure. The residue was purified by column chromatography (2:1 hexane: ethyl acetate) affording a light yellow solid (0.07 g, 43%).
1H-NMR (300 MHz, CDCl3) δ = 8.13 (d, J = 7.8 Hz, 1H), 7.80 (d, J = 7.8 Hz, 1H), 7.42 - 7.26 (m, 2H), 7.13 (s, 1H), 5.77 (bs, 1H), 5.49 (s, 1H), 4.94 (tt, J = 8.9, 4.5 Hz, 1H), 3.84 (ddd, J = 15.8, 12.0, 4.5 Hz, 2H), 3.46 (dddd, J = 12.0, 8.9, 6.2, 3.0 Hz, 2H), 2.38 (s, 3H), 2.32 (s, 3H), 2.16 (s, 3H), 1.96 - 1.56 (m, 4H).
HRMS (IE) m/z calculated C23H25NO4S [M+]: 411.1504, found: 411.1503.
HPLC (99.4%): Rt 22.96 min.

The following examples have been prepared according to Method C:

Example C2: (Tetrahydro-2H-pyran-4-yl)methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Light-yellow solid (0.018 g, 28%).

1H-NMR (300 MHz, CDCl3) δ = 8.12 (d, J = 8.1 Hz, 1H), 7.79 (d, J = 8.1 Hz, 1H), 7.44-7.23 (m, 2H), 7.13 (s, 1H), 5.87 (bs, 1H), 5.48 (s, 1H), 4.09 - 3.57 (m, 4H), 3.53 - 3.08 (m, 2H), 2.36 (s, 3H), 2.32 (s, 3H), 2.16 (s, 3H), 1.88 - 1.52 (m, 1H), 1.52 - 1.08 (m, 4H).

HRMS (IE) m/z calculated C24H27NO4S [M+]: 425.1661, found: 425.1661.

HPLC (98.8%): Rt 17.86 min.

Example C3: Cyclohexyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Light-yellow solid (0.07 g, 36%).

1H-NMR (300 MHz, CDCl3) δ = 8.19 - 8.11 (m, 1H), 7.85 - 7.72 (m, 1H), 7.40 - 7.28 (m, 2H), 7.12 (s, 1H), 5.77 (bs, 1H), 5.49 (s, 1H), 4.77 (dt, J = 9.2, 5.0 Hz, 1H), 2.38 (s, 3H), 2.30 (s, 3H), 2.16 (s, 3H), 1.92 - 1.61 (m, 4H), 1.47 - 1.13 (m, 6H).

HRMS (IE) m/z calculated C24H27NO3S [M+]: 409.1712, found: 409.1714.

HPLC (98.4%): Rt 20.63 min.

Example C4: Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-(2-methoxy-2-oxoethyl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Light-yellow solid (0.148 g, 45%).

1H-NMR (300 MHz, CDCl3) δ = 8.08 (d, J = 8.1 Hz, 1H), 7.80 (d, J = 8.1 Hz, 1H), 7.42 - 7.26 (m, 2H), 7.21 (s, 1H), 6.96 (bs, 1H), 5.50 (s, 1H), 4.02 - 3.76 (m, 2H), 3.73 (s, 3H), 3.63 (s, 3H), 2.39 (s, 3H), 2.15 (s, 3H).

HRMS (IE) m/z calculated C21H21NO5S [M+]: 399.1140, found: 399.1148. HPLC (95.2%): Rt 20.91 min.

Example C5: Methyl 5-acetyl-2,6-dimethyl-4-(2-methylbenzo[b]thiophen-3-yl)-1,4-dihydropyridine-3-carboxylate

Light-yellow solid (0.065 g, 25%).

1H-NMR (300 MHz, CDCl3) δ = 7.83 (d, J = 8.1 Hz, 1H), 7.67 (d, J = 8.1 Hz, 1H), 7.35-7.14 (m, 2H), 5.63 (bs, 1H), 5.59 (s, 1H), 3.49 (s, 3H), 2.50 (s, 3H), 2.29 (s, 3H), 2.25 (s, 3H), 2.07 (s, 3H).

HRMS (IE) m/z calculated C20H21NO3S [M+]: 355.1242, found: 355.1241.

HPLC (98.43%): Rt 18.11 min.

Example C6: Cyclopropylmethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Light-yellow solid (0.065 g, 25%).

1H-NMR (300 MHz, CDCl3) δ = 8.15 (d, J = 8.0 Hz, 1H), 7.79 (d, J = 8.0 Hz, 1H), 7.34 (dt, J = 13.1, 7.4 Hz, 2H), 7.16 (s, 1H), 5.78 (bs, 1H), 5.51 (s, 1H), 3.88 (dd, J = 7.6, 3.9 Hz, 2H), 2.39 (s, 3H), 2.32 (s, 3H), 2.15 (s, 3H), 1.08 (ddt, J = 10.9, 7.6, 3.9 Hz, 1H), 0.51 (d, J = 7.6 Hz, 2H), 0.20 (d, J = 4.9 Hz, 2H).

HRMS (IE) m/z calculated C22H23NO3S [M+]: 381.1399, found: 381.1399.

HPLC (98.9%): Rt 17.72 min.

Example C7: Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-(2-methoxyethyl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Light-yellow solid (0.16 g, 64%).

1H-NMR (300 MHz, CDCl3) δ = 8.09 (d, J = 8.0 Hz, 1H), 7.80 (d, J = 8.0 Hz, 1H), 7.43-7.27 (m, 2H), 7.21 (bs, 1H), 7.16 (s, 1H), 5.49 (s, 1H), 3.63 (s, 3H), 3.61 (d, J = 5.1 Hz, 2H), 3.40 (s, 3H), 3.26 - 3.11 (m, 1H), 3.10 - 2.97 (m, 1H), 2.36 (s, 3H), 2.15 (s, 3H). HRMS (IE) m/z calculated C21H23NO4S [M+]: 385.1348, found: 385.1347.

HPLC (99.7%): Rt 19.65 min.

Example C8: Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-((benzyloxy)methyl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.18 g, 64%).

1H-NMR (300 MHz, CDCl3) δ = 8.09 (d, J = 8.0 Hz, 1H), 7.85 - 7.76 (m, 1H), 7.45 - 7.24 (m, 8H), 7.13 (s, 1H), 5.45 (s, 1H), 4.85 - 4.66 (m, 2H), 4.62 (s, 2H), 3.61 (s, 3H), 2.37 (s, 3H), 2.13 (s, 3H).

HRMS (IE) m/z calculated C26H25NO4S [M+]: 447.1504, found: 447.1490.

HPLC (95.6%): Rt 11.55 min.

Example C9: Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-methyl-2-(phenoxymethyl)-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.11 g, 52%).

1H-NMR (300 MHz, CDCl3) δ = 8.15 - 8.07 (m, 1H), 7.86 - 7.76 (m, 1H), 7.43 - 7.27 (m, 4H), 7.17 (s, 1H), 7.14 (bs, 1H), 7.07 - 6.93 (m, 3H), 5.50 (s, 1H), 5.36 - 5.12 (m, 2H), 3.67 (d, J = 0.6 Hz, 3H), 2.40 (s, 3H), 2.15 (d, 3H).

HRMS (IE) m/z calculated C25H23NO4S [M+]: 433.1348, found: 433.1346.

HPLC (96.0%): Rt 10.20 min.

Example C10: Phenethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.075 g, 21%).

1H-NMR (300 MHz, CDCl3) δ = 7.98 - 7.89 (m, 1H), 7.81 - 7.75 (m, 1H), 7.35 - 7.26 (m, 3H), 7.24 - 7.13 (m, 4H), 7.00 (s, 1H), 5.75 (bs, 1H), 5.40 (s, 1H), 4.38 (dt, J = 10.9, 6.6 Hz, 1H), 4.21 (dt, J = 10.9, 7.2 Hz, 1H), 2.88 (t, J = 6.6 Hz, 2H), 2.37 (s, 3H), 2.23 (s, 3H), 2.10 (s, 3H).

HRMS (IE) m/z calculated C26H25NO3S [M+]: 431.1555, found: 431.1549.

HPLC (97.6%): Rt 17.80 min.

Example C11: Methyl 4-(benzo[b]thiophen-3-yl)-5-(cyclopropanecarbonyl)-6-cyclopropyl-2-methyl-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.080 g, 40 %).

1H-NMR (300 MHz, CDCl3) δ = 8.53 (s, 1H), 8.09 - 7.91 (m, 1H), 7.91 - 7.79 (m, 1H), 7.37 - 7.16 (m, 2H), 7.03 (s, 1H), 5.28 (s, 1H), 3.51 (s, 3H), 2.47 - 2.39 (m, 1H), 2.28 (s, 3H), 2.28 - 2.20 (m, 1H), 1.00 - 0.59 (m, 8H).

HRMS (IE) m/z calculated C23H23NO3S [M+]: 393.1399, found: 393.1403.

HPLC (98.3%): Rt 16.05 min.

Example C14: Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-methyl-2-(trifluoromethyl)-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.012 g, 6%).

1H-NMR (300 MHz, CDCl3) δ = 8.00 (ddd, J = 8.1, 1.3, 0.7 Hz, 1H), 7.83 (ddd, J = 7.8, 1.3, 0.7 Hz, 1H), 7.46 - 7.30 (m, 2H), 7.17 (s, 1H), 6.22 (bs, 1H), 5.51 (s, 1H), 3.65 (s, 3H), 2.43 (s, 3H), 2.11 (s, 3H). 19F-NMR (300 MHz, CDCl3) δ = -63.89 (3F).

HRMS (IE) m/z calculated C19H16F3NO3S [M+]: 395.0803, found: 395.0792.

HPLC (99.5%): Rt 12.57 min.

Example C15 and Example C16: Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-benzyl-2-methyl-1,4-dihydropyridine-3-carboxylate and methyl 4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-5-(2-phenylacetyl)-1,4-dihydropyridine-3-carboxylate

Method C afforded Examples C15 and C16 which were separated by column chromatography.

Example C15: Yellow solid (0.02 g, 6%).

1H-NMR (500 MHz, CDCl3) δ = 8.07 (d, J= 8.2 Hz, 1H), 7.81 (d, J= 8.2 Hz, 1H), 7.41 - 7.26 (m, 7H), 7.14 (s, 1H), 5.70 (bs, 1H), 5.53 (s, 1H), 4.35 (d, J = 16.1 Hz, 1H), 4.11 (d, J = 16.1 Hz, 1H), 3.64 (s, 3H), 2.20 (s, 3H), 2.14 (s, 3H).

HRMS (IE) m/z calculated C25H23NO3S [M+]: 417.1399, found: 417.1400.

HPLC (98.6%): Rt 12.86 min.

Example C16: Yellow solid (0.078 g, 24%).

1H-NMR (500 MHz, CDCl3) δ = 8.13 (d, J = 8.0 Hz, 1H), 7.84 (d, J = 8.0 Hz, 1H), 7.40 (ddd, J = 8.2, 7.0, 1.3 Hz, 1H), 7.33 (ddd, J = 8.2, 7.0, 1.3 Hz, 1H), 7.26 - 7.20 (m, 2H), 7.20 - 7.15 (m, 2H), 7.00 (s, 1H), 6.99 (s, 1H), 5.82 (bs, 1H), 5.60 (s, 1H), 3.91 (d, J = 15.9 Hz, 1H), 3.67 (s, 3H), 3.58 (d, J = 15.9 Hz, 1H), 2.40 (s, 3H), 2.29 (s, 3H).

HRMS (IE) m/z calculated C25H23NO3S [M+]: 417.1399, found: 417.1396.

HPLC (95.4%): Rt 15.69 min.

Example C18: Methyl 4-(benzo[b]thiophen-3-yl)-5-(2-methoxyacetyl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.03 g, 23%).

1H-NMR (300 MHz, CDCl3) δ = 8.10 (d, J = 8.7 Hz, 1H), 7.82 (d, J = 8.7 Hz, 1H), 7.41 (t, J = 7.6 Hz, 1H), 7.33 (t, J = 7.6 Hz, 1H), 7.16 (s, 1H), 5.96 (s, 1H), 5.44 (s, 1H), 4.32 (d, J = 16.1 Hz, 1H), 3.92 (d, J = 16.6 Hz, 1H), 3.66 (s, 3H), 3.28 (s, 3H), 2.43 (s, 3H), 2.31 (s, 3H).

HRMS (IE) m/z calculated C20H21NO4S [M+]: 371.1191, found: 371.1183.

HPLC (97.4%): Rt 22.72 min.

Example C19: Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-(methoxymethyl)-2-methyl-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.062 g, 36%).

1H-NMR (300 MHz, CDCl3) δ = 8.08 (d, J = 8.0 Hz, 1H), 7.81 (d, J = 8.0 Hz, 1H), 7.42 - 7.36 (m, 2H), 7.31 (t, J = 7.5 Hz, 1H), 7.17 (s, 1H), 5.47 (s, 1H), 4.71 (s, 2H), 3.65 (s, 3H), 3.52 (s, 3H), 2.32 (s, 3H), 2.07 (s, 3H).

HRMS (IE) m/z calculated C20H21NO4S [M+]: 371.1191, found: 371.1187.

HPLC (96.0%): Rt 26.61 min.

Example C20: Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-(fluoromethyl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.015 g, 7%).

1H-NMR (300 MHz, CDCl3) δ = 8.09 (d, J = 8.0 Hz, 1H), 7.81 (d, J = 8.0 Hz, 1H), 7.36 (dt, J = 24.7, 7.3 Hz, 2H), 7.18 (s, 1H), 6.72 (d, J = 6.5 Hz, 1H), 5.65 (dd, J = 47.7, 16.3 Hz, 2H), 5.44 (s, 1H), 3.63 (s, 3H), 2.43 (s, 3H), 2.14 (s, 3H). 19F NMR (282 MHz, CDCl3) δ = 122.68 (td, J = 47.7, 6.5 Hz).

HRMS (IE) m/z calculated C19H18FNO3S [M+]: 359.0991, found: 359.0977.

HPLC (96.8%): Rt 13.25 min.

Example C21: Cyclopropylmethyl 5-acetyl-4-(5-fluorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.055 g, 52%).

1H-NMR (300 MHz, CDCl3) δ = 7.82 (dd, J = 10.5, 2.3 Hz, 1H), 7.70 (dd, J = 8.7, 5.0 Hz, 1H), 7.24 (s, 1H), 7.06 (td, J = 8.7, 2.3 Hz, 1H), 5.95 (s, 1H), 5.43 (s, 1H), 3.97 - 3.80 (m, 2H), 2.37 (s, 3H), 2.32 (s, 3H), 2.16 (s, 3H), 1.19 - 1.02 (m, 1H), 0.62 - 0.43 (m, 2H), 0.28 - 0.15 (m, 2H). 19F NMR (282 MHz, CDCl3) δ = -118.91 (s).

HRMS (IE) m/z calculated C22H22FNO3S [M+]: 399.1304, found: 399.1318.

HPLC (98.9%): Rt 17.97 min.

Example C22: 1-(tert-Butoxycarbonyl)piperidin-4-yl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method C using ammonium acetate (1.5 eq) in methanol afforded the title compound as a yellow solid (0.085 g, 21%).

1H-NMR (300 MHz, CDCl3) δ = 8.12 (d, J = 7.8 Hz, 1H), 7.79 (d, J = 7.8 Hz, 1H), 7.34 (dt, J = 12.9, 7.2 Hz, 2H), 7.12 (s, 1H), 5.80 (s, 1H), 5.48 (s, 1H), 4.91 (dt, J = 8.6, 4.4 Hz, 1H), 3.67 (dd, J = 15.3, 7.8 Hz, 2H), 3.07 (dt, J = 10.2, 5.2 Hz, 2H), 2.38 (s, 3H), 2.31 (s, 3H), 2.16 (s, 3H), 1.62 - 1.52 (m, 4H), 1.45 (s, 9H).

HRMS (IE) m/z calculated C28H34N2O5S [M+]: 510.2188, found: 510.2182.

HPLC (99.0%): Rt 21.85 min.

Example C23: Cyclopentylmethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method C using ammonium acetate (1.5 eq) in methanol afforded the title compound as a yellow solid (0.035 g, 14%).
1H-NMR (300 MHz, CDCl3) δ = 8.13 (d, J = 7.9 Hz, 1H), 7.79 (d, J = 7.4 Hz, 1H), 7.43-7.29 (m, 2H), 7.13 (s, 1H), 5.84 (bs, 1H), 5.50 (s, 1H), 3.95 (d, J = 7.3 Hz, 2H), 2.38 (s, 3H), 2.31 (s, 3H), 2.22 - 2.09 (m, 4H), 1.77 - 1.45 (m, 6H), 1.18 - 1.11 (m, 2H). HRMS (IE) m/z calculated C24H27NO3S [M+]: 409.1712, found: 409.1712.
HPLC (99.4%): Rt 16.93 min.

Example C24 Cyclopropylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.035 g, 43%).

1H-NMR (300 MHz, CDCl3) δ = 8.53 - 8.46 (m, 2H), 7.30 (dd, J = 7.9, 4.6 Hz, 1H), 7.23 (s, 1H), 6.53 (s, 1H), 5.47 (s, 1H), 3.95 - 3.73 (m, 2H), 2.37 (s, 3H), 2.32 (s, 3H), 2.17 (s, 3H), 1.03 (ddd, J = 12.7, 8.0, 5.0 Hz, 1H), 0.48 (d, J = 8.0 Hz, 2H), 0.17 (d, J = 4.4 Hz, 2H).

HRMS (IE) m/z calculated C21H22N2O3S [M+]: 382.1351, found: 382.1346.

HPLC (97.9%): Rt 25.95 min.

Example C25: 1-methylpiperidin-4-yl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method C using ammonium acetate (1.5 eq) in methanol afforded the title compound as a yellow solid (0.025 g, 10%).
1H-NMR (300 MHz, CDCl3) δ = 8.13 (d, J = 8.0 Hz, 1H), 7.78 (d, J = 8.0 Hz, 1H), 7.34 (dt, J = 15.9, 7.3 Hz, 2H), 7.11 (s, 1H), 6.03 (s, 1H), 5.49 (s, 1H), 4.78 (tt, J = 8.5, 4.1 Hz, 1H), 2.67 - 2.50(d, J = 17.6 Hz, 2H), 2.35 (s, 3H), 2.30 (s, 3H), 2.27 (d, J = 2.3 Hz, 1H), 2.20 (s, 3H), 2.16 (s, 3H), 2.12 (bs, 1H), 1.96 - 1.83 (m, 2H), 1.80 - 1.59 (m, 2H). HRMS (IE) m/z calculated C24H28N2O3S [M+]: 424.1821, found: 424.1827.
HPLC (96.8%): Rt 33.18 min.

Example C26: Cyclopentyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method C using ammonium acetate (1.5 eq) in methanol afforded the title compound as a yellow solid (0.04 g, 17%).
1H-NMR (300 MHz, CDCl3) δ = 8.14 (d, J = 8.2 Hz, 1H), 7.79 (d, J = 7.7 Hz, 1H), 7.42 - 7.28 (m, 2H), 7.11 (s, 1H), 5.81 (s, 1H), 5.48 (s, 1H), 5.27 - 5.11 (m, 1H), 2.38 (s, 3H), 2.29 (s, 3H), 2.16 (s, 3H), 1.90 - 1.47 (m, 8H).
HRMS (IE) m/z calculated C23H25NO3S [M+]: 395.1555, found: 395.1558.
HPLC (97.1%): Rt 17.56 min.

Example C27: 4,4-dimethylcyclohexyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method C using ammonium acetate (1.5 eq) in methanol afforded the title compound as a light yellow solid (0.03 g, 11 %).
1H-NMR (300 MHz, CDCl3) δ = 8.15 (ddd, J = 8.1, 1.3, 0.7 Hz, 1H), 7.79 (ddd, J = 7.8, 1.3, 0.7 Hz, 1H), 7.40 - 7.28 (m, 2H), 7.13 (s, 1H), 5.78 (bs, 1H), 5.50 (s, 1H), 4.74 (tt, J = 8.8, 4.2 Hz, 1H), 2.37 (s, 3H), 2.31 (s, 3H), 2.16 (s, 3H), 1.80 - 1.66 (m, 1H), 1.66 - 1.50 (m, 3H), 1.46 - 1.27 (m, 3H), 1.27 - 1.16 (m, 1H), 0.88 (s, 3H), 0.88 (s, 3H). HRMS (IE) m/z calculated C26H31NO3S [M+]: 437.2025, found: 437.2028.
HPLC (98.3%): Rt 16.78 min.

Example C28: Cyclobutyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method C using ammonium acetate (1.5 eq) in methanol afforded the title compound as a light yellow solid (0.02 g, 9%).
1H-NMR (300 MHz, CDCl3) δ = 8.14 (d, J = 7.9 Hz, 1H), 7.79 (d, J = 7.9 Hz, 1H), 7.35-7.31 (m, 2H), 7.13 (s, 1H), 6.09 (s, 1H), 5.47 (s, 1H), 5.00 - 4.89 (m, 1H), 2.36 (s, 3H), 2.32 - 2.23 (m, 5H), 2.15 (s, 3H), 2.12 - 1.99 (m, 1H), 1.99 - 1.85 (m, 1H), 1.82 - 1.66 (m, 1H), 1.66 - 1.51 (m, 1H).
HRMS (IE) m/z calculated C22H23NO3S [M+]: 381.1399, found: 381.1408.
HPLC (99.0%): Rt 17.42 min.

Example C29: Methyl 5-acetyl-4-(5-fluorobenzo[b]thiophen-3-yl)-6-methyl-2-(trifluoromethyl)-1,4-dihydropyridine-3-carboxylate

Method C using trifluoroacetic acid (1.5 eq) in methanol afforded the title compound as a yellow solid (0.035 g, 37%).
1H-NMR (300 MHz, CDCl3) δ = 7.71 (dd, J = 9.6, 3.6 Hz, 2H), 7.24 (s, 1H), 7.11 (td, J = 8.7, 2.4 Hz, 1H), 6.30 (s, 1H), 5.43 (s, 1H), 3.69 (s, 3H), 2.43 (s, 3H), 2.13 (s, 3H). 19F-NMR (282 MHz, CDCl3) δ = -63.84 (s, CF3), -118.19 (td, J= 9.6, 4.9 Hz, F).
HRMS (IE) m/z calculated C19H15NO3SF4 [M+]: 413.0709, found: 413.0702.
HPLC (99.1%): Rt 13.51 min.

Example C30: Methyl 5-acetyl-2,6-dimethyl-4-(thieno[3,2-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.015 g, 24%).

1H-NMR (300 MHz, CDCl3) δ = 8.66 (dd, J = 4.5, 1.5 Hz, 1H), 8.07 (dd, J = 8.2, 1.5 Hz, 1H), 7.29 (s, 1H), 7.18 (dd, J = 8.2, 4.5 Hz, 1H), 5.97 (s, 1H), 5.72 (s, 1H), 3.63 (s, 3H), 2.45 (s, 3H), 2.36 (s, 3H), 2.24 (s, 3H).

HRMS (IE) m/z calculated C18H18N2O3S [M+]: 342.1038, found: 342.1034.

HPLC (98.8%): Rt 22.75 min.

Example C31: Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-methyl-6-(trifluoromethyl)-1,4-dihydropyridine-3-carboxylate

Method C using trifluoroacetic acid (1.5 eq) in methanol afforded the title compound as a yellow solid (0.035 g, 24%).
1H-NMR (300 MHz, CDCl3) δ = 7.97 - 7.91 (m, 1H), 7.83 (ddd, J = 7.7, 1.4, 0.7 Hz, 1H), 7.44 - 7.30 (m, 2H), 7.24 (s, 1H), 5.94 (s, 1H), 5.40 (s, 1H), 3.58 (s, 3H), 2.39 (s, 3H), 1.99 (q, J = 0.7 Hz, 3H). 19F-NMR (282 MHz, CDCl3) δ = -63.65. (s, CF3).
HRMS (IE) m/z calculated C19H16NO3SF3 [M+]: 395.0803, found: 395.0810.
HPLC (98.5%): Rt 10.44 min.

Example C32: Cyclopropylmethyl 5-acetyl-4-(5-chlorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.075 g, 50%).

1H-NMR (300 MHz, CDCl3) δ = 8.12 (d, J = 2.0 Hz, 1H), 7.70 (d, J = 9.0 Hz, 1H), 7.28 (d, J = 2.5 Hz, 1H), 7.21 (s, 1H), 5.78 (s, 1H), 5.44 (s, 1H), 3.90 (d, J = 7.3 Hz, 2H), 2.39 (s, 3H), 2.33 (s, 3H), 2.16 (s, 3H), 1.29 - 1.13 (m, 1H), 0.59 - 0.46 (m, 2H), 0.21 (dd, J = 4.8, 1.8 Hz, 2H).

HRMS (IE) m/z calculated C22H22NO3SCI [M+]: 415.1009, found: 415.0997.

HPLC (99.6%): Rt 18.43 min.

Example C33: Cyclohexylmethyl 5-acetyl-4-(5-chlorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.080 g, 61%).

1H-NMR (300 MHz, CDCl3) δ = 8.10 (bs, 1H), 7.68 (d, J = 8.5 Hz, 1H), 7.31 - 7.21 (m, 1H), 7.17 (s, 1H), 6.05 (bs, 1H), 5.42 (s, 1H), 3.90 (ddd, J = 27.5, 10.8, 6.6 Hz, 2H), 2.36 (s, 3H), 2.30 (s, 3H), 2.17 (s, 3H), 1.85 - 1.43 (m, 6H), 1.32 - 1.01 (m, 3H), 1.01 - 0.78 (m, 2H).

HRMS (IE) m/z calculated C25H28NO3SCI [M+]: 457.1478, found: 457.1477.

HPLC (99.1%): Rt 18.16 min.

Example C34: Cyclohexylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.075 g, 58%).

1H-NMR (300 MHz, CDCl3) δ = 8.57 - 8.44 (m, 2H), 7.32 (dd, J = 8.2, 4.6 Hz, 1H), 7.20 (s, 1H), 6.40 (s, 1H), 5.46 (s, 1H), 3.86 (dd, J = 6.1, 3.5 Hz, 2H), 2.37 (s, 3H), 2.33 (s, 3H), 2.18 (s, 3H), 1.70 - 1.44 (m, 6H), 1.16 (d, J = 40.3 Hz, 3H), 0.91 - 0.74 (m, 2H). HRMS (IE) m/z calculated C24H28N2O3S [M+]: 424.1821, found: 424.1833.

HPLC (98.9%): Rt 25.30 min.

Example C35: Cyclopentylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.095 g, 75%).

1H-NMR (300 MHz, CDCl3) 5 = 8.49 (d, J = 8.2 Hz, 2H), 7.31 (dd, J= 8.1, 4.6 Hz, 1H), 7.20 (s, 1H), 6.44 (s, 1H), 5.46 (s, 1H), 4.01 - 3.86 (m, 2H), 2.37 (s, 3H), 2.32 (s, 3H), 2.17 (s, 3H), 2.14 - 2.02 (m, 1H), 1.67 - 1.44 (m, 6H), 1.21 - 1.05 (m, 2H).

HRMS (IE) m/z calculated C23H26N2O3S [M+]: 410.1664, found: 410.1665.

HPLC (98.4%): Rt 25.14 min.

Example C36: Cyclohexyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.028 g, 48%).

1H-NMR (300 MHz, CDCl3) δ = 8.59 - 8.40 (m, 2H), 7.31 (dd, J = 8.2, 4.6 Hz, 1H), 7.19 (s, 1H), 6.47 (s, 1H), 5.46 (s, 1H), 4.75 (tt, J = 8.8, 3.9 Hz, 1H), 2.37 (s, 3H), 2.31 (s, 3H), 2.17 (s, 3H), 1.87 - 1.74 (m, 1H), 1.75 - 1.55 (m, 3H), 1.56 - 1.45 (m, 1H), 1.45 - 1.13 (m, 5H).

HRMS (IE) m/z calculated C23H26N2O3S [M+]: 410.1664, found: 410.1665.

HPLC (98.2%): Rt 24.87 min.

Example C37: Cyclopentyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.034 g, 59%).

1H-NMR (300 MHz, CDCl3) 5 = 8.53 - 8.42 (m, 2H), 7.31 (dd, J = 8.2, 4.6 Hz, 1H), 7.17 (s, 1H), 5.85 (s, 1H), 5.44 (s, 1H), 5.16 (dq, J = 6.1, 3.0 Hz, 1H), 2.38 (s, 3H), 2.31 (s, 3H), 2.17 (s, 3H), 1.80 (td, J = 15.9, 14.5, 6.1 Hz, 2H), 1.67 - 1.46 (m, 6H).

HRMS (IE) m/z calculated C22H24N2O3S [M+]: 396.1508, found: 396.1505.

HPLC (98.9%): Rt 25.70 min.

Example C38: Methyl 5-acetyl-4-(5-chlorobenzo[b]thiophen-3-yl)-6-methyl-2-(trifluoromethyl)-1,4-dihydropyridine-3-carboxylate

Method C using trifluoroacetic acid (1.5 eq) in methanol afforded the title compound as a yellow solid (0.022 g, 51%).
1H-NMR (300 MHz, CDCl3) δ = 8.00 (d, J = 1.6 Hz, 1H), 7.73 (d, J = 8.6 Hz, 1H), 7.31 (dd, J = 8.6, 1.6 Hz, 1H), 7.20 (s, 1H), 6.29 (s, 1H), 5.45 (s, 1H), 3.71 (s, 3H), 2.44 (s, 3H), 2.13 (s, 3H). 19F-NMR (282 MHz, CDCl3) δ = -63.85 (s, CF3).
HRMS (IE) m/z calculated C19H15NO3SF3Cl [M+]: 429.0413, found: 429.0413.
HPLC (95.7%): Rt 13.94 min.

Example C39: Benzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.070 g, 69%).

1H-NMR (300 MHz, CDCl3) δ = 8.50 - 8.44 (m, 1H), 8.26 (dd, J = 8.2, 1.5 Hz, 1H), 7.34 - 7.24 (m, 2H), 7.26 (d, J = 0.8 Hz, 1H), 7.20 - 7.16 (m, 2H), 7.16 - 7.10 (m, 2H), 5.95 (s, 1H), 5.44 (s, 1H), 5.07 (s, 2H), 2.37 (s, 3H), 2.33 (s, 3H), 2.13 (s, 3H).

HRMS (IE) m/z calculated C24H22N2O3S [M+]: 418.1351, found: 418.1358.

HPLC (99.4%): Rt 26.38 min.

Example C40: (Tetrahydro-2H-pyran-4-yl)methyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.029 g, 45%).

1H-NMR (300 MHz, CDCl3) δ = 8.55-8.43 (m, 2H), 7.32 (dd, J = 8.1, 4.6 Hz, 1H), 7.19 (s, 1H), 6.18 (s, 1H), 5.45 (s, 1H), 3.99 - 3.77 (m, 4H), 3.24 (dtd, J = 20.2, 11.6, 2.5 Hz, 2H), 2.37 (s, 3H), 2.34 (s, 3H), 2.18 (s, 3H), 1.47 - 1.15 (m, 5H).

HRMS (IE) m/z calculated C23H26N2O4S [M+]: 426.1613, found: 426.1600.

HPLC (99.5%): Rt 32.56 min.

Example C41: Benzyl 5-acetyl-4-(5-fluorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.018 g, 11%).

1H-NMR (300 MHz, CDCl3) δ = 7.75 - 7.63 (m, 2H), 7.33 - 7.17 (m, 5H), 7.16 (s, 1H), 7.04 (td, J = 8.9, 8.5, 2.0 Hz, 1H), 5.92 (s, 1H), 5.42 (s, 1H), 5.10 (q, J = 12.3 Hz, 2H), 2.35 (s, 3H), 2.31 (s, 3H), 2.13 (s, 3H). 19F-NMR (282 MHz, CDCl3) δ = -118.53 (s, CF). HRMS (IE) m/z calculated C25H22NO3SF [M+]: 435.1304, found: 435.1307.

HPLC (97.1%): Rt 18.43 min.

Example C42: Benzyl 5-acetyl-4-(5-chlorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.065 g, 60%).

1H-NMR (300 MHz, CDCl3) δ = 8.06 (d, J = 1.7 Hz, 1H), 7.68 (d, J = 8.6 Hz, 1H), 7.39-7.18 (m, 6H), 7.13 (s, 1H), 6.00 (s, 1H), 5.44 (s, 1H), 5.27 - 4.96 (m, 2H), 2.35 (s, 3H), 2.30 (s, 3H), 2.13 (s, 3H).

HRMS (IE) m/z calculated C25H22NO3SCl [M+]: 451.1011, found: 451.1011.

HPLC (97.4%): Rt 18.38 min.

Example C43: (Tetrahydro-2H-pyran-4-yl)methyl 5-acetyl-4-(5-chlorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.076 g, 46%).

1H-NMR (300 MHz, CDCl3) δ = 8.10 (d, J = 1.8 Hz, 1H), 7.68 (d, J = 8.1 Hz, 1H), 7.27-7.24 (m, 1H), 7.18 (s, 1H), 6.15 (bs, 1H), 5.39 (s, 1H), 3.93 - 3.82 (m, 4H), 3.50 - 3.19 (m, 2H), 2.35 (s, 3H), 2.17 (s, 3H), 2.03 (s, 3H), 1.89 - 1.63 (m, 2H), 1.51 - 1.47 (m, 1H), 1.35 - 1.17 (m, 2H).

HRMS (IE) m/z calculated C24H26NO4SCl [M+]: 459.1271, found: 459.1272.

HPLC (99.4%): Rt 24.50 min.

Example C44: (Tetrahydro-2H-pyran-4-yl)methyl 5-acetyl-4-(5-fluorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.097 g, 57%).

1H-NMR (300 MHz, CDCl3) δ = 7.79 (d, J = 9.7 Hz, 1H), 7.70 (dd, J = 8.3, 5.1 Hz, 1H), 7.21 (s, 1H), 7.06 (t, J = 8.3 Hz, 1H), 6.02 (s, 1H), 5.40 (s, 1H), 3.99 - 3.73 (m, 4H), 3.28 (dt, J = 25.5, 12.5 Hz, 2H), 2.36 (s, 3H), 2.32 (s, 3H), 2.17 (s, 3H), 1.81 (s, 1H), 1.54 - 1.13 (m, 4H). 19F-NMR (282 MHz, CDCl3) δ = -118.74 (s, CF).

HRMS (IE) m/z calculated C24H26NO4SF [M+]: 443.1567, found: 443.1574.

HPLC (99.6%): Rt 24.69 min

Example C45: Benzyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-methyl-2-(trifluoromethyl)-1,4-dihydropyridine-3-carboxylate

Method C using trifluoroacetic acid (1.5 eq) in methanol afforded the title compound as a yellow solid (0.015 g, 27%).
1H-NMR (300 MHz, CDCl3) δ = 7.97 - 7.90 (m, 1H), 7.85 - 7.77 (m, 1H), 7.35 - 7.29 (m, 2H), 7.29 - 7.22 (m, 3H), 7.16 - 7.07 (m, 3H), 6.18 (s, 1H), 5.53 (s, 1H), 5.07 (q, J = 12.2 Hz, 2H), 2.42 (s, 3H), 2.10 (s, 3H). 19F-NMR (282 MHz, CDCl3) δ = -63.64 (s, CF3).
HRMS (IE) m/z calculated C25H20NO3SF3 [M+]: 471.1116, found: 471.1117.
HPLC (99.3%): Rt 12.46 min

Example C46: Pyridin-4-ylmethyl 5-acetyl-4-(5-fluorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.091 g, 55%).

1H-NMR (300 MHz, CDCl3) δ = 8.50 - 8.42 (m, 2H), 7.76 - 7.63 (m, 2H), 7.21 (s, 1H), 7.04 (td, J = 8.7, 2.5 Hz, 1H), 6.96 (d, J = 5.2 Hz, 2H), 6.13 (s, 1H), 5.45 (s, 1H), 5.11 (q, J = 13.7 Hz, 2H), 2.36 (s, 3H), 2.35 (s, 3H), 2.17 (s, 3H). 19F-NMR (282 MHz, CDCl3) δ = -118.54 (s, CF).

HRMS (IE) m/z calculated C24H21N2O3SF [M+]: 436.1257, found: 436.1255.

HPLC (99.5%): Rt 26.10 min.

Example C47: 4-Fluorobenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.050 g, 43%).

1H-NMR (300 MHz, CDCl3) 5 = 8.49 (d, J = 4.5 Hz, 1H), 8.28 (dd, J = 8.2, 1.6 Hz, 1H), 7.21 - 7.07 (m, 4H), 6.95 (t, J = 8.6 Hz, 2H), 6.06 (s, 1H), 5.43 (s, 1H), 5.11 - 4.93 (m, 2H), 2.37 (s, 3H), 2.33 (s, 3H), 2.14 (s, 3H). 19F-NMR (282 MHz, CDCl3) δ = -113.87 (s, CF).

HRMS (IE) m/z calculated C24H21N2O3SF [M+]: 436.1257, found: 436.1259.

HPLC (98.5%): Rt 20.40 min.

Example C48: Pyridin-3-ylmethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method C using ammonium acetate (1.5 eq) in methanol afforded the title compound as a yellow solid (0.025 g, 15%).
1H-NMR (300 MHz, CDCl3) δ = 8.50 (d, J = 11.7 Hz, 2H), 8.01 - 7.96 (m, 1H), 7.85 - 7.66 (m, 1H), 7.40 (d, J = 7.9 Hz, 1H), 7.32 - 7.21 (m, 2H), 7.17 (t, J = 6.5 Hz, 1H), 7.10 (s, 1H), 6.14 (s, 1H), 5.46 (s, 1H), 5.08 (d, J = 3.1 Hz, 2H), 2.34 (s, 3H), 2.30 (s, 3H), 2.12 (s, 3H).
HRMS (IE) m/z calculated C24H22N2O3S [M+]: 418.1351, found: 418.1346.
HPLC (98.0%): Rt 24.79 min.

Example C51: 1,1'-(4-(benzo[b]thiophen-3-yl)-2-benzyl-6-methyl-1,4-dihydropyridine-3,5-diyl)bis(ethan-1-one)

Yield: 8 % (18 mg, yellow solid).

1H-NMR (300 MHz, CDCl3) δ = 8.12 (d, J = 7.8 Hz, 1H), 7.80 (d, J = 8.5 Hz, 1H), 7.45-7.28 (m, 5H), 7.32 - 7.19 (m, 2H), 7.09 (s, 1H), 5.85 (s, 1H), 5.66 (s, 1H), 4.15 (dd, J = 16.5 Hz, J = 41.8 Hz, 1H), 2.26 (s, 3H), 2.24 (s, 3H), 2.17 (s, 3H).

HRMS (IE) m/z calculated C25H23NO2S [M+]: 401.1450, found: 401.1442

Example C52: 1-(5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-yl)-2-phenylethan-1-one

Light-yellow solid (35 mg, 15%).

1H-NMR (300 MHz, CDCl3) δ = 8.12 (d, J = 8.1 Hz, 1H), 7.80 (d, J = 7.9 Hz, 1H), 7.47-7.25 (m, 5H), 7.22 (d, J = 8.3 Hz, 2H), 7.09 (s, 1H), 5.85 (s, 1H), 5.66 (s, 1H), 4.15 (dd, J = 94.6, 16.2 Hz, 2H), 2.26 (s, 3H), 2.24 (s, 3H), 2.17 (s, 3H).

HRMS (IE) m/z calculated C25H23NO2S [M+]: 401.1450, found: 401.1437.

Example C53: Methyl 5-acetyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-2-(trifluoromethyl)-1,4-dihydropyridine-3-carboxylate

Method C using 1.5 eq of TFA yielded the title compound as a yellow solid (30 mg, 26%).
1H-NMR (300 MHz, CDCl3) δ = 8.56 (bs, 1H), 8.36 (dd, J = 8.2, 1.3 Hz, 1H), 7.36 (dd, J = 8.2, 4.5 Hz, 1H), 7.23 (s, 1H), 6.52 (bs, 1H), 5.49 (s, 1H), 3.66 (s, 3H), 2.44 (s, 3H), 2.16 (s, 3H). 19F-NMR (282 MHz, CDCl3) δ = -63.72 (CF3).
HRMS (IE) m/z calculated C18H15N2O3SF3 [M+]: 396.0755, found: 396.0768.

Example C54: 1-(2-methyl-5-(piperidine-1-carbonyl)-4-(thieno[2,3-b]pyridin-3-yl)-6-(trifluoromethyl)-1,4-dihydropyridin-3-yl)ethan-1-one

Method C using 1.5 eq of TFA yielded the title compound as a yellow solid (10 mg, 15%).
1H-NMR (300 MHz, CDCl3) δ = 8.60 (dd, J = 4.6, 1.6 Hz, 1H), 8.13 (dd, J = 8.2, 1.6 Hz, 1H), 7.42 - 7.31 (m, 2H), 5.65 (s, 1H), 5.47 (s, 1H), 3.35 - 3.14 (m, 2H), 2.80 - 2.65 (m, 1H), 2.41 (s, 3H), 2.26 - 2.12 (m, 1H), 1.93 (s, 3H), 1.39 - 1.22 (m, 2H), 1.23 - 1.04 (m, 2H), 1.06 - 0.82 (m, 2H). 19F-NMR (282 MHz, CDCl3) δ = -67.14 (CF3).
HRMS (IE) m/z calculated C22H22N3O2SF3 [M+]: 449.1385, found: 449.1378.

Example C55: 4-(((5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carbonyl)oxy)methyl)benzoic acid

Method C gave 4-(tert-butoxycarbonyl)benzyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate (yellow solid, 30 mg, 66%). 1H-NMR (300 MHz, CDCl3) δ = 8.00 (d, J = 8.1 Hz, 1H), 7.88 (d, J = 8.6 Hz, 2H), 7.79 (d, J = 8.1 Hz, 1H), 7.32 - 7.22 (m, 3H), 7.19 (d, J = 7.9 Hz, 2H), 5.86 (s, 1H), 5.49 (s, 1H), 5.11 (s, 2H), 2.37 (s, 3H), 2.31 (s, 3H), 2.13 (s, 3H), 1.60 (s, 9H).

Hydrolysis of the ester: A mixture of 4-(tert-butoxycarbonyl)benzyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate (0.05 g, 0.09 mmol) in dichloromethane (3 mL) was stirred at 0 °C. Trifluoroacetic acid (0,5 mL) in dichloromethane (2 mL) was dropwise added at the same temperature during 60 min. The mixture was allowed to warm up to RT and the solvent was removed under reduced pressure. The residue was purified by column chromatography (5% DCM in methanol) affording a yellow solid (0.012 g, 27%).
1H-NMR (300 MHz, CDCl3) δ = 17.14 (s, 1H), 9.14 (s, 1H), 8.02 (dd, J = 4.8, 3.0 Hz, 1H), 7.89 (dd, J = 6.2, 3.0 Hz, 1H), 7.81 (d, J = 7.9 Hz, 2H), 7.30 - 7.24 (m, 2H), 7.20 (s, 1H), 7.12 (d, J = 7.9 Hz, 2H), 5.46 (s, 1H), 5.05 (s, 2H), 2.31 (s, 3H), 2.27 (s, 3H), 2.11 (s, 3H).
HRMS (IE) m/z calculated C26H23NO5SNa [M+Na]: 484.1189, found: 484.1188.

Example C56: Benzyl 5-acetyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-2-(trifluoromethyl)-1,4-dihydropyridine-3-carboxylate

Method C using 1.5 eq of TFA in dioxane yielded the title compound as a yellow solid (35 mg, 12%).
1H-NMR (300 MHz, CDCl3) δ = 8.51 (s, 1H), 8.23 (d, J = 8.1 Hz, 1H), 7.32 - 7.07 (m, 7H), 6.45 (s, 1H), 5.49 (s, 1H), 5.09 (d, J = 2.6 Hz, 2H), 2.43 (s, 3H), 2.14 (s, 3H). 19F-NMR (282 MHz, CDCl3) δ = -63.44 (CF3).
HRMS (IE) m/z calculated C24H19N2O3SF3 [M+]: 472.1068, found: 472.1067.

Example C57: Pyridin-3-ylmethyl 5-acetyl-4-(5-fluorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (60 mg, 50%).
1H-NMR (300 MHz, CDCl3) δ = 8.52 (d, J = 4.3 Hz, 1H), 8.48 (s, 1H), 7.72 - 7.63 (m, 2H), 7.46 (d, J = 7.8 Hz, 1H), 7.24 - 7.18 (m, 1H), 7.17 (s, 1H), 7.04 (td, J = 8.7, 2.5 Hz, 1H), 6.27 (s, 1H), 5.39 (s, 1H), 5.11 (s, 2H), 2.34 (s, 3H), 2.30 (s, 3H), 2.14 (s, 3H). 19F-NMR (282 MHz, CDCl3) δ = -118.51 (q, J = 9.2 Hz, CF).
HRMS (IE) m/z calculated C24H21N2O3SF [M+]: 436.1257, found: 436.1252

Example C58: Pyridin-3-ylmethyl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (68 mg, 49%).
1H-NMR (300 MHz, CDCl3) δ = 8.52 (s, 2H), 7.98 (d, J = 7.9 Hz, 1H), 7.44 (d, J = 7.5 Hz, 1H), 7.36 (d, J = 7.1 Hz, 1H), 7.23 - 7.10 (m, 3H), 5.93 (s, 1H), 5.41 (s, 1H), 5.08 (q, J = 12.8 Hz, 2H), 2.36 (s, 3H), 2.32 (s, 3H), 2.13 (s, 3H).
HRMS (IE) m/z calculated C24H21N2O3SBr [M+]: 496.0456, found: 496.0462.

Example C59: Pyridin-3-ylmethyl 5-acetyl-4-(7-cyanobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (30 mg, 31%).
1H-NMR (300 MHz, CDCl3) δ = 8.52 (d, J = 15.9 Hz, 2H), 8.29 (d, J = 8.2 Hz, 1H), 7.64 (d, J = 8.2 Hz, 1H), 7.43 - 7.29 (m, 2H), 7.25 - 7.14 (m, 2H), 6.00 (s, 1H), 5.48 (s, 1H), 5.07 (q, J = 13.7, 13.0 Hz, 2H), 2.38 (s, 3H), 2.36 (s, 3H), 2.15 (s, 3H).
HRMS (IE) m/z calculated C25H21N3O3S [M+]: 443.1304, found: 443.1288.

Example C60: Pyridin-3-ylmethyl 5-acetyl-4-(5-chlorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (68 mg, 35%).
1H-NMR (300 MHz, CDCl3) δ = 8.52 (s, 2H), 8.04 (s, 1H), 7.69 (d, J = 8.6 Hz, 1H), 7.49 (d, J = 7.8 Hz, 1H), 7.29-7.19 (m, 2H), 7.15 (s, 1H), 6.00 (s, 1H), 5.41 (s, 1H), 5.13 (s, 2H), 2.36 (s, 3H), 2.32 (s, 3H), 2.14 (s,3H).
HRMS (IE) m/z calculated C24H21N2O3SCl [M+]: 452.0961, found: 452.0959.

Example C61: Pyridin-3-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (35 mg, 34%).
1H-NMR (300 MHz, CDCl3) δ = 8.62 - 8.40 (m, 3H), 8.29 (d, J = 8.0 Hz, 1H), 7.40 (d, J = 8.0 Hz, 1H), 7.23 - 7.14 (m, 3H), 6.05 (s, 1H), 5.43 (s, 1H), 5.09 (q, J = 12.6 Hz, 2H), 2.38 (s, 3H), 2.35 (s, 3H), 2.15 (s, 3H).
HRMS (IE) m/z calculated C23H21N3O3S [M+]: 419.1304, found: 419.1306.

Example C62: Pyridin-4-ylmethyl 5-acetyl-4-(5-chlorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (35 mg, 48%).
1H-NMR (300 MHz, CDCl3) δ = 8.46 (d, J = 4.7 Hz, 2H), 8.04 (s, 1H), 7.70 (d, J = 8.5 Hz, 1H), 7.23 - 7.16 (m, 2H), 6.99 (d, J = 5.0 Hz, 2H), 6.00 (s, 1H), 5.46 (s, 1H), 5.22 - 5.03 (m, 2H), 2.38 (s, 3H), 2.35 (s, 3H), 2.17 (s, 3H).
HRMS (IE) m/z calculated C24H21N2O3SCl [M+]: 452.0961, found: 452.0954.

Example C63: 4-(cyclopropylcarbamoyl)benzyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (30 mg, 13%).
1H-NMR (300 MHz, CDCl3) δ = 8.00 (dd, J = 6.9, 2.4 Hz, 1H), 7.84 - 7.75 (m, 1H), 7.61 (d, J = 8.2 Hz, 2H), 7.34 - 7.24 (m, 1H), 7.28 - 7.20 (m, 1H), 7.18 (d, J = 8.2 Hz, 2H), 7.10 (s, 1H), 6.18 (bs, 1H), 5.87 (bs, 1H), 5.49 (s, 1H), 5.10 (s, 2H), 2.90 (dt, J = 6.9, 3.6 Hz, 1H), 2.37 (s, 3H), 2.31 (s, 3H), 2.13 (s, 3H), 0.93 - 0.82 (m, 2H), 0.67 - 0.57 (m, 2H).
HRMS (IE) m/z calculated C29H29N2O4S [M+1]: 501.1843, found: 501.1841.

Example C64: Pyridin-4-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (28 mg, 40%).
1H-NMR (300 MHz, CDCl3) δ = 8.51 - 8.41 (m, 3H), 8.37 (dd, J = 8.2, 1.6 Hz, 1H), 7.22 - 7.15 (m, 2H), 6.96 (d, J = 5.5 Hz, 2H), 5.49 (s, 1H), 5.28 (s, 1H), 5.04 (q, J = 15.0 Hz, 2H), 2.35 (s, 6H), 2.18 (s, 3H).
HRMS (IE) m/z calculated C23H21N3O3S [M+]: 419.1304, found: 419.1304.

Example C65: 4-bromobenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (131 mg, 72%).
1H-NMR (300 MHz, CDCl3) δ = 8.50 (d, J = 4.7 Hz, 1H), 8.34 (s, 1H), 7.38 (d, J = 8.0 Hz, 2H), 7.18 (s, 2H), 6.99 (d, J = 8.0 Hz, 2H), 6.00 (s, 1H), 5.43 (s, 1H), 4.99 (q, J = 12.5 Hz, 2H), 2.39 (s, 3H), 2.36 (s, 3H), 2.16 (s, 3H).
HRMS (IE) m/z calculated C24H21N2O3SBr [M+]: 496.0456, found: 496.0441.

Example C66: 3-bromobenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (115 mg, 54%). 1H-NMR (300 MHz, CDCl3) δ = 8.50 (dd, J = 4.6, 1.5 Hz, 1H), 8.31 (d, J = 8.1 Hz, 1H), 7.48 - 7.37 (m, 1H), 7.33 (t, J = 1.5 Hz, 1H), 7.23 - 7.01 (m, 4H), 5.97 (s, 1H), 5.44 (s, 1H), 5.02 (q, J = 12 Hz, 2H), 2.38 (s,3), 2.36 (s, 3H), 2.15 (s, 3H).
HRMS (IE) m/z calculated C24H21N2O3SBr [M+]: 496.0456, found: 496.0456.

Example C67: 2-bromobenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (127 mg, 74%). 1H-NMR (300 MHz, CDCl3) δ = 8.46 (dd, J = 4.7, 1.6 Hz, 1H), 8.32 (d, J = 8.2 Hz, 1H), 7.57 - 7.48 (m, 1H), 7.23 - 7.05 (m, 5H), 6.04 (s, 1H), 5.47 (s, 1H), 5.14 (q, J = 15 Hz, 2H), 2.39 (s, 3H), 2.38 (s, 3H), 2.16 (s, 3H).
HRMS (IE) m/z calculated C24H21N2O3SBr [M+]: 496.0456, found: 496.0446.

Example C68: (3-fluoropyridin-4-yl)methyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (30 mg, 47%).
1H-NMR (300 MHz, CDCl3) δ = 8.50 (d, J = 4.6 Hz, 1H), 8.44 - 8.30 (m, 2H), 8.23 (s, 1H), 7.20 (q, J = 4.9 Hz, 2H), 6.88 (t, J = 5.4 Hz, 1H), 6.18 (s, 1H), 5.47 (s, 1H), 5.24 - 5.09 (m, 2H), 2.38 (s, 3H), 2.37 (s, 3H), 2.18 (s, 3H). 19F-NMR (282 MHz, CDCl3) δ = - 132.30 (s, CF).
HRMS (IE) m/z calculated C23H20N3O3SF [M+]: 437.1209, found: 437.1215.

Example C69: Pyrimidin-5-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (35 mg, 58%).
1H-NMR (300 MHz, CDCl3) δ = 9.13 (s, 1H), 8.56 (s, 2H), 8.50 (dd, J = 4.6, 1.6 Hz, 1H), 8.33 (dd, J = 8.2, 1.6 Hz, 1H), 7.23 (dd, J = 8.3, 4.6 Hz, 1H), 7.18 (s, 1H), 6.51 (s, 1H), 5.42 (s, 1H), 5.08 (q, J = 12 Hz, 2H), 2.36 (s, 3H), 2.33 (s, 3H), 2.16 (s, 3H).
HRMS (IE) m/z calculated C22H20N4O3S [M+]: 420.1256, found: 420.1262.

Example C70: (5-bromopyridin-3-yl)methyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (84 mg, 63%).
1H-NMR (300 MHz, CDCl3) δ = 8.58 (s, 1H), 8.50 (d, J = 4.3 Hz, 1H), 8.38 (s, 1H), 8.32 (d, J = 7.8 Hz, 1H), 7.54 (s, 1 H), 7.22 (dd, J = 4.3, 7.8 Hz, 1H), 7.18 (s, 1H), 6.32 (bs, 1H), 5.43 (s, 1H), 5.04 (q, J = 13 Hz, 2H), 2.37 (s, 3H), 2.35 (s, 3H), 2.16 (s, 3H).
HRMS (IE) m/z calculated C23H20N3O3SBr [M+]: 497.0409, found: 497.0412.

Example C71: 2-phenylpropan-2-yl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (17 mg, 27%).
1H-NMR (300 MHz, CDCl3) δ = 8.60 - 8.47 (m, 1H), 8.36 (dd, J = 8.2, 1.6 Hz, 1H), 7.23 - 7.09 (m, 5H), 7.08 - 6.99 (m, 2H), 6.02 (s, 1H), 5.49 (s, 1H), 2.38 (s, 3H), 2.31 (s, 3H), 2.15 (s, 3H), 1.70 (s, 3H), 1.64 (s, 3H).
HRMS (IE) m/z calculated C26H27N2O3S [M+1]: 447.1737, found: 447.1736.

Example C72: 3-Cyanobenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (38 mg, 58%).
1H-NMR (300 MHz, CDCl3) δ = 8.55 - 8.45 (m, 1H), 8.34 (dd, J = 8.2, 1.5 Hz, 1H), 7.54 (dt, J = 7.4, 1.5 Hz, 1H), 7.45 (s, 1H), 7.37 - 7.27 (m, 2H), 7.24 - 7.20 (m, 1H), 7.19 (s, 1H), 6.40 (s, 1H), 5.45 (s, 1H), 5.06 (q, J = 12 Hz, 2H), 2.37 (s, 3H), 2.35 (s, 3H), 2.17 (s, 3H).
HRMS (IE) m/z calculated C25H21N3O3S [M+]: 443.1304, found: 443.1310.

Example C73: 4-cyanobenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (25 mg, 40%).
1H-NMR (300 MHz, CDCl3) δ = 8.55 - 8.45 (m, 1H), 8.34 (dd, J = 8.2, 1.5 Hz, 1H), 7.54 (dt, J = 7.4, 1.5 Hz, 1H), 7.45 (s, 1H), 7.37 - 7.27 (m, 2H), 7.24 - 7.20 (m, 1H), 7.19 (s, 1H), 6.40 (s, 1H), 5.45 (s, 1H), 5.19 - 4.93 (q, J = 12 Hz, 2H), 2.37 (s, 3H), 2.35 (s, 3H), 2.17 (s, 3H).
HRMS (IE) m/z calculated C25H21N3O3S [M+]: 443.1304, found: 443.1306.

Example C74: (6-chloropyridin-3-yl)methyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (80 mg, 54%).
1H-NMR (300 MHz, CDCl3) δ = 8.57 - 8.46 (m, 1H), 8.37 (d, J = 8.2 Hz, 1H), 8.26 (d, J = 2.4 Hz, 1H), 7.28 (d, J = 2.7 Hz, 1H), 7.22 (d, J = 4.6 Hz, 1H), 7.20 - 7.12 (m, 2H), 6.16 (s, 1H), 5.41 (s, 1H), 5.13 - 4.93 (q, J = 12 Hz, 2H), 2.38 (s, 3H), 2.36 (s, 3H), 2.17 (s, 3H).
HRMS (IE) m/z calculated C23H20N3O3SCl [M+]: 453.0914, found: 453.0894.

Example C75: 3-morpholinobenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (50 mg, 69%).
1H-NMR (300 MHz, CDCl3) δ = 8.51 - 8.45 (m, 1H), 8.32 (d, J = 8.1 Hz, 1H), 7.24 - 7.11 (m, 3H), 6.89 (d, J = 8.1 Hz, 1H), 6.74 (d, J = 6.9 Hz, 2H), 5.92 (s, 1H), 5.45 (s, 1H), 5.04 (d, J = 3.0 Hz, 2H), 3.88 (d, J = 4.8 Hz, 4H), 3.09 (t, J = 4.8 Hz, 4H), 2.39 (s, 3H), 2.35 (s, 3H), 2.14 (s, 3H).
HRMS (IE) m/z calculated C28H29N3O4S [M+]: 503.1879, found: 503.1878.

Example C76: 4,4-dimethylcyclohexyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (45 mg, 73%).
1H-NMR (300 MHz, CDCl3) δ = 8.57 - 8.45 (m, 2H), 7.31 (dd, J = 8.1, 4.7 Hz, 1H), 7.20 (s, 1H), 6.90 (s, 1H), 5.46 (s, 1H), 4.71 (tt, J = 8.8, 4.1 Hz, 1H), 2.35 (s, 3H), 2.31 (s, 3H), 2.17 (s, 3H), 1.70 - 1.49 (m, 3H), 1.35 - 1.17 (m, 5H), 0.86 (s, 3H), 0.83 (s, 3H).
HRMS (IE) m/z calculated C25H30N2O3S [M+]: 438.1977, found: 438.1967.

Example C77: (2-chloropyridin-4-yl)methyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (117 mg, 75%).
1H-NMR (300 MHz, CDCl3) δ = 8.53 (s, 1H), 8.41 (d, J = 8.2 Hz, 1H), 8.22 (d, J = 5.1 Hz, 1H), 7.22 (s, 2H), 7.01 (d, J = 7.4 Hz, 1H), 6.85 (d, J = 5.1 Hz, 1H), 6.14 (s, 1H), 5.49 (s, 1H), 5.13 - 4.96 (q, J = 12 Hz, 2H), 2.40 (s, 6H), 2.19 (s, 3H).
HRMS (IE) m/z calculated C23H20N3O3SCl [M+]: 453.0914, found: 453.0927.

Example C78: Tetrahydro-2H-pyran-4-yl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (25 mg, 51%).
1H-NMR (300 MHz, CDCl3) δ = 8.50 (d, J = 8.1 Hz, 2H), 7.33 (dd, J = 8.1, 4.7 Hz, 1H), 7.24 - 7.17 (m, 1H), 6.70 (s, 1H), 5.46 (s, 1H), 4.92 (tt, J = 8.9, 4.2 Hz, 1H), 3.81 (ddt, J = 16.6, 11.5, 4.2 Hz, 2H), 3.46 (ddt, J = 12.0, 5.3, 2.9 Hz, 2H), 2.36 (s, 3H), 2.33 (s, 3H), 2.18 (s, 3H), 1.92 - 1.83 (m, 1H), 1.80 - 1.71 (m, 1H), 1.69 - 1.57 (m, 1H), 1.53 - 1.40 (m, 1H).
HRMS (IE) m/z calculated C22H24N2O4S [M+]: 412.1457, found: 412.1457.

Example C79: 4,4-difluorocyclohexyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (45 mg, 83%).
1H-NMR (300 MHz, CDCl3) δ = 8.57 - 8.48 (m, 2H), 7.33 (dd, J = 7.9, 4.8 Hz, 1H), 7.20 (s, 1H), 6.96 (s, 1H), 5.46 (s, 1H), 4.90 (s, 1H), 2.35 (s, 3H), 2.32 (s, 3H), 2.19 (s, 3H), 1.96 - 1.58 (m, 8H). 19F-NMR (282 MHz, CDCl3) δ = -95.77 (d, J = 247.3 Hz CF).
HRMS (IE) m/z calculated C23H24N2O3SF2 [M+]: 446.1476, found: 446.1475.

Example C80: 5-Acetyl-N-benzyl-N,2,6-trimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxamide

Method C yielded the title compound as a yellow solid (37 mg, 67%).
1H-NMR (300 MHz, CDCl3) δ = 8.51 (d, J = 4.6 Hz, 1H), 8.16 - 8.06 (m, 1H), 7.25 - 7.15 (m, 5H), 7.08 - 6.98 (m, 2H), 6.12 (s, 1H), 5.37 (s, 1H), 4.65 (d, J = 14.1 Hz, 1H), 4.07 (d, J = 14.1 Hz, 1H), 2.36 (s, 3H), 2.13 (s, 3H), 1.98 (s, 3H), 1.68 (s, 3H).
HRMS (IE) m/z calculated C25H25N3O2S [M+]: 431.1667, found: 431.1675.

Example C81: Oxetan-3-yl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (5.2 mg, 6%).
1H-NMR (300 MHz, CDCl3) δ = 8.12 (d, J = 8.0 Hz, 1H), 7.48 (d, J = 7.9 Hz, 1H), 7.30 (d, J = 7.9 Hz, 1H), 7.18 (s, 1H), 5.73 (s, 1H), 5.45 (s, 1H), 5.40 (q, J = 5.9 Hz, 1H), 4.89 - 4.80 (m, 2H), 4.61 - 4.49 (m, 2H), 2.55 (tt, J = 8.6, 5.7 Hz, 1H), 2.36 (s, 3H), 2.16 (s, 3H), 1.01 (ddp, J = 18.2, 8.6, 4.6 Hz, 2H), 0.69 (dq, J = 7.4, 4.6, 4.0 Hz, 2H).
HRMS (IE) m/z calculated C23H22NO4SBr [M+]: 487.0453, found: 487.0439.

Example C82: Isopropyl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (21 mg, 14%).
1H-NMR (300 MHz, CDCl3) δ = 8.14 (d, J = 8.1 Hz, 1H), 7.46 (d, J = 7.6 Hz, 1H), 7.30 (s, 1H), 7.24 (s, 1H), 7.15 (s, 1H), 5.68 (s, 1H), 5.44 (s, 1H), 5.03 (p, J = 6.2 Hz, 1H), 2.53 (ddd, J = 8.8, 5.7, 3.4 Hz, 1H), 2.36 (s, 3H), 2.14 (s, 3H), 1.21 (d, J = 6.2 Hz, 3H), 1.12 (d, J = 6.2 Hz, 3H), 1.01 - 0.88 (m, 2H), 0.67 (dqd, J = 11.1, 5.7, 3.4 Hz, 2H).
HRMS (IE) m/z calculated C23H24NO3SBr [M+]: 473.0660, found: 473.0677.

Example C83: Methyl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-6-methyl-2-(trifluoromethyl)-1,4-dihydropyridine-3-carboxylate

Method C using 1.5 eq of TFA in MeOH yielded the title compound as a yellow solid (8 mg, 18%).
1H-NMR (300 MHz, CDCl3) δ = 8.00 (d, J = 8.1 Hz, 1H), 7.51 (d, J = 7.5 Hz, 1H), 7.35-7.28 (m, 1H), 7.24 (s, 1H), 6.20 (bs, 1H), 5.47 (s, 1H), 3.65 (s, 3H), 2.44 (s, 3H), 2.12 (s, 3H). 19F-NMR (282 MHz, CDCl3) δ = 63.85 (s, CF3).
HRMS (IE) m/z calculated C19H15NO3SF3Br [M+]: 472.9908, found: 472.9915.

Example C84: Cyclopropylmethyl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate

Method C in dioxane yielded the title compound as a yellow solid (20 mg, 13%). 1H-NMR (300 MHz, CDCl3) δ = 8.15 (d, J = 8.1 Hz, 1H), 7.46 (d, J = 7.6 Hz, 1H), 7.29-7.23 (m, 1H), 7.17 (s, 1H), 5.70 (bs, 1H), 5.47 (bs, 1H), 3.95 - 3.86 (m, 2H), 2.59 (tt, J = 8.7, 5.7 Hz, 1H), 2.36 (s, 3H), 2.14 (s, 3H), 1.12 - 0.87 (m, 3H), 0.73 - 0.59 (m, 2H), 0.57 - 0.44 (m, 2H), 0.26 - 0.14 (m, 2H).
HRMS (IE) m/z calculated C24H24NO3SBr [M+]: 485.0660, found: 485.0676.

Example C85: Methyl 5-acetyl-2-cyclopropyl-6-methyl-4-(7-(2,2,2-trifluoroacetyl) benzo[b]thiophen-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (42 mg, 44%).
1H-NMR (300 MHz, CDCl3) δ = 8.64 (d, J = 8.1 Hz, 1H), 8.17 (d, J = 7.4 Hz, 1H), 7.62 (t, J = 7.8 Hz, 1H), 7.32 (s, 1H), 5.89 (bs, 1H), 5.54 (s, 1H), 3.64 (s, 3H), 2.59 (tt, J = 8.7, 5.6 Hz, 1H), 2.36 (s, 3H), 2.14 (s, 3H), 1.06 - 0.85 (m, 2H), 0.81 - 0.58 (m, 2H).
HRMS (IE) m/z calculated C23H20NO4SF3 [M+]: 463.1065, found: 463.1054.

Example C86: 2-phenylpropan-2-yl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (18 mg, 18%).
1H-NMR (300 MHz, CDCl3) δ = 8.06 (d, J = 8.1 Hz, 1H), 7.46 (d, J = 7.6 Hz, 1H), 7.21 - 7.10 (m, 5H), 7.07 - 6.98 (m, 2H), 5.87 (bs, 1H), 5.49 (s, 1H), 2.36 (s, 3H), 2.28 (s, 3H), 2.14 (s, 3H), 1.72 (s, 3H), 1.64 (s, 3H).
HRMS (ESI) m/z calculated C27H26NO3SBr [M+]: 546.0709, found: 546.0711.
HPLC (98.2%): Rt 18.30 min.

Example C87: Methyl 5-acetyl-4-(7-chlorobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (80 mg, 69%).
1H-NMR (300 MHz, CDCl3) δ = 8.07 - 7.99 (m, 1H), 7.39 - 7.28 (m, 2H), 7.15 (d, J = 1.7 Hz, 1H), 5.67 (s, 1H), 5.45 (s, 1H), 3.66 (s, 3H), 2.59 (tt, J = 8.4, 5.7 Hz, 1H), 2.35 (s, 3H), 2.13 (s, 3H), 1.04 - 0.90 (m, 2H), 0.66 (dtt, J = 9.0, 5.7, 3.4 Hz, 2H).
HRMS (IE) m/z calculated C21H20NO3SCl [M+]: 401.0852, found: 401.0855.
HPLC (99.1%): Rt 18.66 min.

Example C88: methyl 5-acetyl-2-cyclopropyl-6-methyl-4-(7-(trifluoromethyl)benzo[b]thiophen-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (55 mg, 80%).
1H-NMR (300 MHz, CDCl3) δ = 8.35 (d, J = 8.2 Hz, 1H), 7.62 (d, J = 7.5 Hz, 1H), 7.49 (t, J = 7.9 Hz, 1H), 7.20 (s, 1H), 5.66 (bs, 1H), 5.51 (s, 1H), 3.65 (s, 3H), 2.59 (h, J = 6.9, 6.2 Hz, 1H), 2.36 (s, 3H), 2.13 (s, 3H), 1.09 - 0.84 (m, 2H), 0.7509 - 0.57 (m, 2H). 19F-NMR (282 MHz, CDCl3) δ (ppm): -62.96 (s, CF3).
HRMS (IE) m/z calculated C22H20NO3SF3 [M+]: 435.1116, found: 435.1129.
HPLC (99.4%): Rt 19.68 min.

Example C89: 3-chlorobenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (78 mg, 53%).
1H-NMR (300 MHz, CDCl3) δ = 8.49 (dd, J = 4.6, 1.6 Hz, 1H), 8.29 (dd, J = 8.2, 1.6 Hz, 1H), 7.28 - 7.23 (m, 1H), 7.22 - 7.13 (m, 4H), 7.01 (dt, J = 7.6, 1.6 Hz, 1H), 6.26 (s, 1H), 5.44 (s, 1H), 5.09 - 4.97 (m, 2H), 2.37 (s, 3H), 2.34 (s, 6H), 2.15 (s, 3H).
HRMS (IE) m/z calculated C24H21N2O3SCl [M+]: 452.0961, found: 452.0966.
HPLC (98.6%): Rt 19.83 min.

Example C90: 2-phenylpropan-2-yl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (20 mg, 17%).
1H-NMR (300 MHz, CDCl3) δ = 8.05 (d, J = 8.0 Hz, 1H), 7.52 - 7.40 (m, 1H), 7.22 - 7.13 (m, 4H), 7.12 (s, 1H), 7.10 - 7.04 (m, 2H), 5.67 (bs, 1H), 5.50 (s, 1H), 2.63 - 2.47 (m, 1H), 2.35 (s, 3H), 2.13 (s, 3H), 1.72 (s, 3H), 1.67 (s, 3H), 1.03 - 0.77 (m, 2H), 0.76 - 0.57 (m, 2H).
HRMS (ESI) m/z calculated C29H28NO3SBrNa [M+Na]: 572.0865, found: 572.0865.
HPLC (98.82%): Rt 17.16 min.

Example C91: Cyclohexyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (70 mg, 79%).
1H-NMR (300 MHz, CDCl3) δ = 8.52 (s, 1H), 8.46 (d, J = 8.1 Hz, 1H), 7.31 (dd, J = 8.1, 4.7 Hz, 1H), 7.13 (s, 1H), 5.69 (bs, 1H), 5.47 (s, 1H), 4.83 - 4.71 (m, 1H), 2.64 - 2.47 (m, 1H), 2.36 (s, 3H), 2.16 (s, 3H), 1.90 - 1.70 (m, 2H), 1.68 - 1.50 (m, 4H), 1.35 - 1.19 (m, 4H), 1.08 - 0.87 (m, 2H), 0.78 - 0.58 (m, 2H).
HRMS (IE) m/z calculated C25H28N2O3S [M+]: 436.1821, found: 436.1841.
HPLC (99.3%): Rt 18.47 min.

Example C92: 2-phenylpropan-2-yl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (15 mg, 16 %).
1H-NMR (300 MHz, CDCl3) δ = 8.50 (dd, J = 4.6, 1.6 Hz, 1H), 8.33 (dd, J = 8.2, 1.6 Hz, 1H), 7.23 - 7.12 (m, 4H), 7.11 (s, 1H), 7.12 - 7.02 (m, 2H), 5.71 (s, 1H), 5.49 (s, 1H), 2.65 - 2.49 (m, 1H), 2.36 (s, 3H), 2.13 (s, 3H), 1.70 (s, 3H), 1.67 (s, 3H), 1.03 - 0.83 (m, 2H), 0.76 - 0.58 (m, 2H).
HRMS (ESI) m/z calculated C28H29N2O3S [M+1]: 473.1893, found: 473.1891.
HPLC (95.7%): Rt 25.13 min.

Example C93: 1-(4-(7-Bromobenzo[b]thiophen-3-yl)-5-(cyclopropanecarbonyl)-6-cyclopropyl-2-methyl-1,4-dihydropyridin-3-yl)ethan-1-one

Method C yielded the title compound as a yellow solid (80 mg, 56 %).
1H-NMR (300 MHz, CDCl3) δ = 8.09 (d, J = 8.1 Hz, 1H), 7.46 (d, J = 7.6 Hz, 1H), 7.28 (d, J = 8.1 Hz, 1H), 7.10 (s, 1H), 5.84 (bs, 1H), 5.50 (s, 1H), 2.41 (s, 3H), 2.38 - 2.27 (m, 1H), 2.20 - 2.01 (m, 4H), 1.41 - 1.22 (m, 1H), 1.20 - 1.06 (m, 1H), 1.07 - 0.90 (m, 3H), 0.84 - 0.65 (m, 3H).
HRMS (IE) m/z calculated C23H22NO2SBr [M+]: 455.0555, found: 455.0540.
HPLC (99.1%): Rt 22.48 min

Example C94: 3-chlorobenzyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (16 mg, 13 %).
1H-NMR (300 MHz, CDCl3) δ = 8.53 (d, J = 4.6 Hz, 1H), 8.32 (d, J = 8.1 Hz, 1H), 7.32 - 7.28 (m, 1H), 7.25 - 7.18 (m, 3H), 7.16 (s, 1H), 7.07 (d, J = 7.4 Hz, 1H), 5.79 (bs, 1H), 5.50 (s, 1H), 5.16 - 5.04 (m, 2H), 2.73 - 2.51 (m, 1H), 2.39 (s, 3H), 2.19 (s, 3H), 1.08 - 0.87 (m, 2H), 0.83 - 0.61 (m, 2H).
HRMS (IE) m/z calculated C26H23N2O3SCl [M+]: 478.1118, found: 478.1118.
HPLC (96.7%): Rt 19.79 min.

Example C95: 2-(4-fluorophenyl)propan-2-yl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (10 mg, 7 %).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.52 (d, J = 3.9 Hz, 1H), 8.35 (dd, J = 8.3, 1.6 Hz, 1H), 7.21 (dd, J = 8.2, 4.6 Hz, 1H), 7.16 (s, 1H), 7.00 - 6.91 (m, 2H), 6.87 - 6.75 (m, 2H), 5.90 (s, 1H), 5.48 (s, 1H), 2.38 (s, 3H), 2.30 (s, 3H), 2.15 (s, 3H), 1.68 (s, 3H), 1.61 (s, 3H). 19F-NMR (282 MHz, CDCl3) δ (ppm): -116.44 (CF).
HRMS (IE) m/z calculated C26H25N2O3SF [M+]: 464.1570, found: 464.1570.
HPLC (98.2%): Rt 19.52 min.

Example C96: 2-(4-fluorophenyl)propan-2-yl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (30 mg, 17 %).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.03 (d, J = 8.1 Hz, 1H), 7.47 (d, J = 7.6 Hz, 1H), 7.17 (t, J = 7.9 Hz, 1H), 7.12 (s, 1H), 6.98 (dd, J = 8.7, 5.4 Hz, 2H), 6.80 (t, J = 8.7 Hz, 2H), 5.61 (s, 1H), 5.48 (s, 1H), 2.62 - 2.45 (m, 1H), 2.35 (s, 3H), 2.13 (s, 3H), 1.69 (s, 3H), 1.65 (s, 3H), 1.04 - 0.82 (m, 2H), 0.76 - 0.59 (m, 2H). 19F-NMR (282 MHz, CDCl3) δ (ppm): -116.65 (CF).
HRMS (IE) m/z calculated C29H27N2O3SBrFNa [M+Na]: 590.0771, found: 590.0772.
HPLC (98.0%): Rt 18.52 min.

Example C97: 2-(4-fluorophenyl)propan-2-yl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (38 mg, 24 %).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.55 - 8.44 (m, 1H), 8.33 (dd, J = 8.2, 1.3 Hz, 1H), 7.19 (dd, J = 8.2, 4.6 Hz, 1H), 7.10 (s, 1H), 7.03 - 6.93 (m, 2H), 6.79 (t, J = 8.7 Hz, 2H), 5.97 (bs, 1H), 5.46 (s, 1H), 2.60 - 2.43 (m, 1H), 2.34 (s, 3H), 2.12 (s, 3H), 1.66 (s, 3H), 1.63 (s, 3H), 1.00 - 0.80 (m, 2H), 0.78 - 0.56 (m, 2H). 19F-NMR (282 MHz, CDCl3) δ (ppm): -116.53 (CF).
HRMS (IE) m/z calculated C28H28N2O3SF [M+]: 491.1799, found: 491.1800.
HPLC (98.9%): Rt 19.26 min.

Example C98: Methyl 5-acetyl-2-cyclopropyl-4-(7-fluorobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (94 mg, 71 %).
1H-NMR (300 MHz, CDCl3) δ (ppm): 7.89 (d, J = 8.2 Hz, 1H), 7.35 (td, J = 8.0, 5.2 Hz, 1H), 7.13 (s, 1H), 7.01 (dd, J = 9.7, 8.0 Hz, 1H), 5.63 (bs, 1H), 5.47 (s, 1H), 3.67 (s, 3H), 2.60 (tt, J = 8.7, 5.7 Hz, 1H), 2.36 (s, 3H), 2.14 (s, 3H), 1.08 - 0.85 (m, 2H), 0.74 - 0.56 (m, 2H). 19F-NMR (282 MHz, CDCl3) δ (ppm): -116.08 (CF).
HRMS (IE) m/z calculated C21H20NO3SF [M+]: 385.1148, found: 385.1132.
HPLC (99.3%): Rt 22.58 min.

Example C99: Cyclopropylmethyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C in dioxane yielded the title compound as a yellow solid (12 mg, 26 %).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.53 - 8.50 (m, 1H), 8.46 (dd, J = 8.2, 1.6 Hz, 1H), 7.29 (dd, J = 8.2, 4.6 Hz, 1H), 7.17 (s, 1H), 5.71 (bs, 1H), 5.48 (s, 1H), 3.90 (dd, J = 7.3, 2.7 Hz, 2H), 2.60 (tt, J = 8.6, 5.7 Hz, 1H), 2.37 (s, 3H), 2.16 (s, 3H), 1.05 - 0.91 (m, 3H), 0.72 - 0.61 (m, 2H), 0.55 - 0.43 (m, 2H), 0.27 - 0.12 (m, 2H).
HRMS (IE) m/z calculated C23H24N2O3S [M+]: 408.1508, found: 408.1509.
HPLC (99.7%): Rt 30.16 min.

Example C100: Cyclopentyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method C in dioxane yielded the title compound as a yellow solid (27 mg, 14 %).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.52 (dd, J = 4.6, 1.5 Hz, 1H), 8.46 (dd, J = 8.2, 1.5 Hz, 1H), 7.30 (dd, J = 8.2, 4.6 Hz, 1H), 7.12 (s, 1H), 5.71 (bs, 1H), 5.45 (s, 1H), 5.24 - 5.11 (m, 1H), 2.62 - 2.45 (m, 1H), 2.36 (s, 3H), 2.16 (s, 3H), 1.92 - 1.72 (m, 2H), 1.64 - 1.44 (m, 6H), 1.07 - 0.85 (m, 2H), 0.76 - 0.56 (m, 2H).
HRMS (IE) m/z calculated C24H26N2O3S [M+]: 422.1664, found: 422.1662.
HPLC (99.7%): Rt 30.24 min.

Example C101: Cyclopropylmethyl 5-acetyl-4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate

Method C in dioxane yielded the title compound as a yellow solid (9 mg, 26 %). 1H-NMR (300 MHz, CDCl3) δ (ppm): 8.56 - 8.41 (m, 1H), 7.66 (d, J = 7.5 Hz, 1H), 7.46 (t, J = 7.5 Hz, 1H), 7.24 (s, 1H), 5.71 (bs, 1H), 5.53 (s, 1H), 3.98 - 3.77 (m, 2H), 2.70 - 2.52 (m, 1H), 2.37 (s, 3H), 2.16 (s, 3H), 1.09 - 0.92 (m, 3H), 0.77 - 0.58 (m, 2H), 0.53 - 0.39 (m, 2H), 0.27 - 0.10 (m, 2H).
HRMS (IE) m/z calculated C25H24N2O3S [M+]: 432.1508, found: 432.1516.
HPLC (97.8%): Rt 22.16 min.

Example C102: Methyl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2-cyclobutyl-6-methyl-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (41 mg, 29 %).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.09 (d, J = 8.8 Hz, 1H), 7.47 (d, J = 7.5 Hz, 1H), 7.29 (d, J = 8.0 Hz, 1H), 7.18 (s, 1H), 6.10 (bs, 1H), 5.41 (s, 1H), 3.63 (s, 3H), 2.42 (s, 3H), 2.33 - 2.17 (m, 2H), 2.14 (s, 3H), 2.06 - 1.78 (m, 5H).
HRMS (IE) m/z calculated C22H22NO3SBr [M+]: 459.0504, found: 459.0505.
HPLC (95.2%): Rt 18.21 min.

Example C103: Dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Method C yielded the title compound as a yellow solid (80 mg, 65 %).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.36 (d, J = 9.2 Hz, 1H), 7.66 (d, J = 7.3 Hz, 1H), 7.46 (dd, J = 9.2, 7.3 Hz, 1H), 7.24 (s, 1H), 5.64 (bs, 1H), 5.47 (s, 1H), 3.56 (s, 3H), 3.54 (s, 3H), 2.77 - 2.67 (m, 1H), 2.34 (s, 3H), 1.07 - 0.96 (m, 2H), 0.77 - 0.65 (m, 2H).
HRMS (IE) m/z calculated C22H20N2O4S [M+]: 408.1144, found: 408.1153.
HPLC (99.2%): Rt 12.64 min.

Example C104: Methyl 5-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (40 mg, 48 %).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.24 (d, J = 9.1 Hz, 1H), 7.71 (d, J = 7.3 Hz, 1H), 7.56 - 7.44 (m, 1H), 7.23 (s, 1H), 5.73 (bs, 1H), 5.14 (s, 1H), 3.55 (s, 3H), 2.87 - 2.71 (m, 1H), 2.09 (s, 3H), 1.13 - 0.99 (m, 2H), 0.82 - 0.65 (m, 2H).
HRMS (IE) m/z calculated C21H17N3O2S [M+]: 375.1041, found: 375.1046.
HPLC (99.0%): Rt 21.27 min.

Example C105: Methyl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2-cyclopentyl-6-methyl-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (59 mg, 40 %).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.06 (d, J = 8.0 Hz, 1H), 7.47 (d, J = 7.4 Hz, 1H), 7.29 (d, J = 8.0 Hz, 1H), 7.19 (s, 1H), 5.88 (bs, 1H), 5.45 (s, 1H), 4.26 - 4.02 (m, 1H), 3.63 (s, 3H), 2.38 (s, 3H), 2.15 (s, 3H), 2.04 - 1.90 (m, 2H), 1.78 - 1.69 (m, 4H), 1.53 - 1.38 (m, 2H).
HRMS (IE) m/z calculated C23H24NO3SBr [M+]: 473.0660, found: 473.0647.
HPLC (97.4%): Rt 12.81 min.

Example C106: Methyl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2-cyclohexyl-6-methyl-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (89 mg, 59 %).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.05 (d, J = 8.8 Hz, 1H), 7.46 (d, J = 7.5 Hz, 1H), 7.28 (d, J = 7.5 Hz, 1H), 7.18 (s, 1H), 6.00 (bs, 1H), 5.44 (s, 1H), 3.80 - 3.66 (m, 1H), 3.62 (s, 3H), 2.38 (s, 3H), 2.15 (s, 3H), 1.92 - 1.69 (m, 5H), 1.44 - 1.20 (m, 5H). HRMS (IE) m/z calculated C24H26NO3SBr [M+]: 487.0817, found: 487.0801.
HPLC (96.7%): Rt 11.94 min.

Example C107: Methyl 5-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (15 mg, 13 %).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.25 (dd, J = 8.2, 0.9 Hz, 1H), 7.71 (d, J = 7.3 Hz, 1H), 7.55 - 7.45 (m, 1H), 7.20 (s, 1H), 5.57 (bs, 1H), 5.14 (s, 1H), 3.56 (s, 3H), 2.87 - 2.70 (m, 1H), 1.93 - 1.78 (m, 1H), 1.16 - 0.83 (m, 5H), 0.80 - 0.63 (m, 3H).
HRMS (IE) m/z calculated C23H19N3O2S [M+]: 401.1198, found: 401.1199.
HPLC (96.5%): Rt 15.42 min.

Example C108: 3-((4-methylpiperazin-1-yl)methyl)benzyl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

Method C yielded the title compound as a yellow solid (33 mg, 18 %).
1H-NMR (300 MHz, CDCl3) δ (ppm): 7.98 (d, J = 8.2 Hz, 1H), 7.43 (d, J = 7.6 Hz, 1H), 7.27 - 7.04 (m, 6H), 5.80 (bs, 1H), 5.44 (s, 1H), 5.07 (s, 2H), 3.44 (s, 2H), 2.48 - 2.41 (m, 4H), 2.37 (s, 3H), 2.32 (s, 3H), 2.28 (s, 3H), 2.12 (s, 3H), 1.73 - 1.60 (m, 4H). HRMS (IE) m/z calculated C31H34N3O3SBr [M+]: 607.1504, found: 607.1491.
HPLC (98.4%): Rt 9.94 min.

Example C109: 3-(cyclopropylmethyl) 5-methyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

The title compound was prepared using a variation of Method C by heating in dioxane in a sealed tube at 130 °C overnight yielding a yellow solid (35 mg, 28 %).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.40 (d, J = 8.2 Hz, 1H), 7.65 (d, J = 7.0 Hz, 1H), 7.44 (t, J = 7.7 Hz, 1H), 7.26 (s, 1H), 5.61 (bs, 1H), 5.49 (s, 1H), 3.84 - 3.73 (m, 2H), 3.55 (s, 3H), 2.81 - 2.65 (m, 1H), 2.34 (s, 3H), 1.02 (dd, J = 8.9, 5.5 Hz, 2H), 0.95 - 0.80 (m, 1H), 0.72 (d, J = 6.1 Hz, 2H), 0.38 (t, J = 9.7 Hz, 2H), 0.06 (s, 2H).
HRMS (IE) m/z calculated C25H24N2NaO4S [M+]: 471.1349, found: 471.1347.

Example C110: 3-(cyclopropylmethyl) 5-methyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate

The title compound was prepared using a variation of Method C by heating in dioxane in a sealed tube at 130 °C overnight yielding a yellow solid (20 mg, 15 %).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.41 (d, J = 8.3 Hz, 1H), 7.65 (d, J = 7.4 Hz, 1H), 7.44 (t, J = 7.8 Hz, 1H), 7.23 (s, 1H), 5.56 (bs, 1H), 5.51 (s, 1H), 3.79 (d, J = 7.3 Hz, 2H), 3.57 (s, 3H), 2.85 - 2.61 (m, 2H), 1.09 - 0.84 (m, 5H), 0.75 - 0.55 (m, 4H), 0.47 - 0.29 (m, 2H), 0.15 - -0.02 (m, 2H).
HRMS (IE) m/z calculated C27H26N2NaO4S [M+Na]: 497.1505, found: 497.1503.

Example C111: Cyclopropylmethyl 5-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate

The title compound was prepared using a variation of Method C by heating the reaction mixture in MeOH in a sealed tube at 130 °C for 7h yielding a yellow solid (8 mg, 7%).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.24 (d, J = 8.3 Hz, 1H), 7.70 (d, J = 7.4 Hz, 1H), 7.48 (t, J = 7.8 Hz, 1H), 7.27 (s, 1H), 5.73 (bs, 1H), 5.19 (s, 1H), 3.75 (d, J = 7.3 Hz, 2H), 2.92 - 2.70 (m, 1H), 2.09 (s, 3H), 1.14 - 0.97 (m, 2H), 0.88 - 0.66 (m, 3H), 0.40 - 0.12 (m, 2H), 0.08 - -0.17 (m, 2H)
HRMS (IE) m/z calculated C24H21N3NaO2S [M+Na]: 438.1247, found: 438.1245.

Example C112: Dimethyl 2-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-6-cyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate

The title compound was prepared in three steps following Y. Satoh et al., Chem. Pharm. Bull. 1991, 39, 3189-3201.

In a first step, dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-(dimethoxymethyl)-1,4-dihydropyridine-3,5-dicarboxylate was prepared using a variation of Method C by heating the reaction mixture in methanol in a sealed tube at 130 °C for 90 min yielding a yellow solid (130 mg, 48 %).

In a second step, dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-(dimethoxymethyl)-1,4-dihydropyridine-3,5-dicarboxylate (0.100 g, 0.21 mmol) was stirred in acetone (4 mL) and hydrochloride acid 4N (0.53 mL, 2.13 mmol) at room temperature for 60 minutes. The solvent was removed under reduced pressure, and the residue was dissolved in ethyl acetate and washed with toluene, saturated bicarbonate solution and brine. The organic phase was dried (Na2SO4), filtered and concentrated yielding dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-formyl-1,4-dihydropyridine-3,5-dicarboxylate that was used in the next step without further purification.

In a third step, a mixture of dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-formyl-1,4-dihydropyridine-3,5-dicarboxylate, sodium acetate (0.026 g, 0.32 mmol) and hydroxylamine hydrochloride (0.018 g, 0.25 mmol) in acetic acid (1 mL) was stirred at room temperature for 60 minutes. Acetic anhydride (0.142 mL, 1.48 mmol) was added and stirred at room temperature for 60 minutes and then at 95°C for 120 minutes. The solvent was removed under reduced pressure, and the residue was taken up in ethyl acetate and washed with toluene, saturated bicarbonate solution and brine. The organic phase was dried (Na2SO4), filtered and concentrated and the residue purified by column chromatography (4:1 hexane: ethyl acetate) affording the title compound dimethyl 2-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-6-cyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate as a yellow solid (0.030 g, 34%).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.33 (d, J = 8.3 Hz, 1H), 7.72 (d, J = 7.3 Hz, 1H), 7.58 - 7.49 (m, 1H), 7.35 (s, 1H), 6.73 (bs, 1H), 5.52 (s, 1H), 3.69 (s, 3H), 3.64 (s, 1H), 3.55 (s, 3H), 3.03 - 2.86 (m, 2H), 2.21 - 2.07 (m, 2H).
HRMS (IE) m/z calculated C22H18N3O4S [M+1]: 420.1013, found: 420.1012.

Example C113: Dimethyl 2-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Following the 3-step procedure described above for the synthesis of Example C112, the title compound was obtained as a yellow solid (0.046 g, 41 %).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.35 (d, J = 8.3 Hz, 1H), 7.71 (d, J = 7.3 Hz, 1H), 7.58 - 7.47 (m, 1H), 7.36 (s, 1H), 6.70 (bs, 1H), 5.52 (s, 1H), 3.69 (s, 3H), 3.56 (s, 3H), 2.42 (s, 3H).
HRMS (IE) m/z calculated C20H14N3O4S [M+1]: 392.0700, found: 392.0698.

Example C114: Methyl 5-acetyl-2-((2-aminoethoxy)methyl)-4-(benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

In a first step, methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-((2-(1,3-dioxoisoindolin-2-yl)ethoxy)methyl)-6-methyl-1,4-dihydropyridine-3-carboxylate was prepared using a variation of Method C by heating in methanol in a sealed tube at 130 °C for 7h yielding a yellow solid (120 mg, 60 %).

Deprotection: In a second step, methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-((2-(1,3-dioxoisoindolin-2-yl)ethoxy)methyl)-6-methyl-1,4-dihydropyridine-3-carboxylate (0.060 g, 0.11 mmol) and hydrazine monohydrate (0.018 g, 0.56 mmol) were heated in ethanol (3 mL) at 78°C for 5 minutes. The mixture was allowed to cool to RT and after addition of hydrazine monohydrate (0.018 g, 0.56 mmol) was stirred at room temperature overnight. The mixture was filtered, concentrated and the residue purified by column chromatography (dichloromethane: methanol 10%) affording a yellow solid (0.020 g, 44%).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.15 - 8.01 (m, 2H), 7.80 (d, J = 7.6 Hz, 1H), 7.34 (dt, J = 24.5, 6.8 Hz, 2H), 7.15 (bs, 1H), 5.45 (bs, 1H), 4.85 - 4.49 (m, 2H), 3.62 (s, 3H), 3.57 (t, J = 5.0 Hz, 2H), 2.97 (bs, 2H), 2.42 (s, 3H), 2.13 (s, 3H), 1.78 (bs, 3H).

Example C115: Methyl 5-acetyl-2-((2-aminoethoxy)methyl)-4-(7-bromobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Following the 2-step procedure described above for the synthesis of Example C114, the title compound was obtained as a yellow solid (0.030 g, 33 %).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.10 (d, J = 7.5 Hz, 2H), 7.46 (d, J = 7.3 Hz, 1H), 7.31 - 7.25 (m, 1H), 7.22 (s, 1H), 5.40 (s, 1H), 4.85 - 4.56 (m, 2H), 3.60 (s, 5H), 2.99 (s, 2H), 2.42 (s, 5H), 2.13 (s, 3H).

Example C116: Methyl 5-acetyl-2-((2-aminoethoxy)methyl)-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Following the 2-step procedure described above for the synthesis of Example C114, the title compound was obtained as a yellow solid (0.035 g, 51 %).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.41 (d, J = 8.2 Hz, 1H), 8.27 (bs, 1H), 7.66 (d, J= 7.2 Hz, 1H), 7.47 (t, J = 7.8 Hz, 1H), 7.27 (s, 1H), 5.47 (s, 1H), 4.85 - 4.56 (m, 2H), 3.59 (s, 5H), 2.98 (s, 2H), 2.42 (s, 3H), 2.15 (s, 3H), 1.83 (s, 2H).
HRMS (IE) m/z calculated C22H24N3O4S [M+1]: 426.1482, found: 426.1480.

Examples C117 and C118 have been prepared according to the following 3-step synthesis:

Example C117: Dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-formyl-1,4-dihydropyridine-3,5-dicarboxylate

In a first step, dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-(dimethoxymethyl)-1,4-dihydropyridine-3,5-dicarboxylate was prepared using a variation of Method C by heating in methanol in a sealed tube at 130°C for 1,5h yielding a yellow solid (0.130 g, 48%).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.36 (d, J = 8.3 Hz, 1H), 7.67 (d, J = 7.4 Hz, 1H), 7.47 (t, J = 7.8 Hz, 1H), 7.30 (s, 1H), 6.80 (s, 1H), 5.98 (s, 1H), 5.52 (s, 1H), 3.59 (s, 3H), 3.58 (s, 3H), 3.44 (s, 3H), 3.43 (s, 3H), 2.89 - 2.79 (m, 1H), 1.11 - 0.89 (m, 2H), 0.91 - 0.58 (m, 2H).

Deprotection: In a second step, a mixture of dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-(dimethoxymethyl)-1,4-dihydropyridine-3,5-dicarboxylate (0.100 g, 0.21 mmol) and hydrochloride acid 4N (0.53 mL, 2.13 mmol) in acetone (4 mL) was stirred at room temperature for 60 minutes. The solvent was removed under reduced pressure and the residue was taken up in ethyl acetate, washed with toluene, saturated bicarbonate solution and brine and then dried (Na2SO4) and concentrated yielding the title compound that was used without further purification.
1H-NMR (300 MHz, CDCl3) δ (ppm): 10.50 (s, 1H), 8.33 (d, J = 8.3 Hz, 1H), 7.71 (d, J = 7.0 Hz, 1H), 7.51 (t, J = 7.8 Hz, 1H), 7.32 (s, 1H), 7.26 (s, 1H), 5.59 (s, 1H), 3.68 (s, 3H), 3.62 (d, J = 6.2 Hz, 1H), 3.57 (s, 3H), 2.98 (t, J = 7.6 Hz, 2H), 2.17 - 2.05 (m, 2H).
HRMS (APCI) m/z calculated C22H19N2O5S [M+1]: 423.1009, found: 423.1007.

Example C118: Dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-(hydroxymethyl)-1,4-dihydropyridine-3,5-dicarboxylate

Reduction: In a third step, sodium borohydride (0.005 g, 0.13 mmol) was added to a mixture of dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-formyl-1,4-dihydropyridine-3,5-dicarboxylate (0.047 g, 0.11 mmol) in ethanol (3 mL) at 0°C which was stirred at same temperature for 1h. Then water was added, and the mixture was extracted with ethyl acetate. The organic phase was separated, dried (Na2SO4), filtered and concentrated, and the resulting residue was purified by column chromatography (2:1 hexane: ethyl acetate) affording the title compound as a yellow solid (0.026 g, 56%).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.33 (d, J = 7.9 Hz, 1H), 7.67 (d, J = 7.2 Hz, 1H), 7.52 - 7.45 (m, 2H), 7.30 (s, 1H), 5.46 (s, 1H), 4.82 (bs, 2H), 3.61 (t, J = 6.2 Hz, 2H), 3.54 (s, 3H), 3.53 (s, 3H), 2.93 (t, J = 7.4 Hz, 2H), 2.86 (bs, 1H), 2.17 - 2.05 (m, 2H).

Example C119: Dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-formyl-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

The title compound was prepared following the synthesis described for Example C117 yielding a solid that was used without further purification.
1H-NMR (300 MHz, CDCl3) δ (ppm): 10.50 (s, 1H), 8.35 (d, J = 8.3 Hz, 1H), 7.70 (d, J = 7.3 Hz, 1H), 7.55 - 7.46 (m, 1H), 7.33 (s, 1H), 7.10 (bs, 1H), 5.58 (s, 1H), 3.67 (s, 3H), 3.57 (s, 3H), 2.45 (s, 3H).
HRMS (APCI) m/z calculated C20H17N2O5S [M+1]: 397.0853, found: 397.0853.

Example C120: Dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-(hydroxymethyl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

The title compound was prepared following the synthesis described for Example C118 yielding a yellow solid (0.007 g, 16%).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.35 (d, J = 8.5 Hz, 1H), 7.66 (d, J = 7.2 Hz, 1H), 7.47 (t, J = 7.7 Hz, 1H), 7.30 (s, 1H), 7.24 (bs, 1H), 5.45 (s, 1H), 4.83 (d, J = 2.1 Hz, 2H), 3.54 (s, 3H), 3.53 (s, 3H), 2.41 (s, 3H), 1.61 (s, 1H).
HRMS (APCI) m/z calculated C20H19N2O5S [M+1]: 399.1009, found: 399.1010.

Example C121: Dimethyl 2-cyano-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate

The title compound was prepared from methyl (Z)-4,4-dimethoxy-3-oxo-2-(thieno[2,3-b]pyridin-3-ylmethylene)butanoate following the 3-step synthesis described for Examples C117 and C118 yielding a yellow solid (0.025 g).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.33 (d, J = 8.3 Hz, 1H), 7.72 (d, J = 7.3 Hz, 1H), 7.58 - 7.49 (m, 1H), 7.35 (s, 1H), 6.73 (bs, 1H), 5.52 (s, 1H), 3.69 (s, 3H), 3.64 (s, 1H), 3.55 (s, 3H), 3.03 - 2.86 (m, 2H), 2.21 - 2.07 (m, 2H).
HRMS (APCI) m/z calculated C22H18N3O4S [M+1]: 420.1013, found: 420.1012.

Example C122: Cyclopropylmethyl 5-acetyl-4-(6-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate

The title compound was prepared using a variation of Method C by heating in dioxane in a sealed tube at 130 °C for 2 h yielding a yellow solid (0.010 g, 10%).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.30 (d, J = 9.1 Hz, 1H), 8.11 (s, 1H), 7.59 (d, J = 9.1 Hz, 1H), 7.36 (s, 1H), 5.71 (bs, 1H), 5.54 (s, 1H), 3.88 (dd, J = 7.3, 2.1 Hz, 2H), 2.68 - 2.54 (m, 1H), 2.36 (s, 3H), 2.15 (s, 3H), 1.08 - 0.93 (m, 3H), 0.74 - 0.64 (m, 2H), 0.54 - 0.42 (m, 2H), 0.22 - 0.12 (m, 2H).
HRMS (APCI) m/z calculated C25H25N2O3S [M+1]: 433.1580, found: 433.1581.

Example C123: Cyclopropylmethyl 2,5-diacetyl-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

The title compound was prepared using a variation of Method C by heating in dioxane in a sealed tube at 130 °C for 2 h yielding a yellow solid (0.006 g, 11%).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.47 (d, J = 8.2 Hz, 1H), 7.66 (d, J = 7.3 Hz, 1H), 7.46 (t, J = 7.6 Hz, 1H), 7.29 (s, 1H), 5.86 (bs, 1H), 5.52 (s, 1H), 3.91 - 3.78 (m, 2H), 2.40 (s, 3H), 2.35 (s, 3H), 2.16 (s, 3H), 0.26 - 0.07 (m, 1H), 1.09 - 0.95 (m, 2H), 0.57 - 0.38 (m, 2H).

Method D Example D1: Methyl 5-acetyl-2-amino-4-(benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

A mixture of 3-(benzo[b]thiophen-3-ylmethylene)pentane-2,4-dione (0.15 g, 0.61 mmol, 1 eq), methyl 3-amino-3-iminopropanoate (0.093 g, 0.61 mmol, 1 eq) and piperidine (0.073 g, 0.74 mmol, 1.2 eq) in i-PrOH (5 ml) was stirred at reflux temperature for 4 hours. The reaction mixture was concentrated in vacuum, and the precipitate that formed was filtered off, washed with methanol, dichloromethane and ether and dried affording the title compound as a yellow solid (65 mg, 31%).
1H-NMR (300 MHz, CDCl3) δ = 8.00 (s, 1H), 7.22 (d, J = 7.8 Hz, 1H), 7.06 (d, J = 7.9 Hz, 1H), 6.63 - 6.43 (m, 2H), 6.40 (s, 1H), 5.80 (s, 2H), 4.46 (s, 1H), 2.66 (s, 3H), 1.46 (s, 3H), 1.24 (s, 3H).
HRMS (IE) m/z calculated C18H18N2O3S [M+]: 342.1038, found: 342.1044.

Example D2: Methyl 2-amino-4-(benzo[b]thiophen-3-yl)-5-(cyclopropanecarbonyl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method D yielded the title compound as a light-yellow solid (40 mg, 48 %).
1H-NMR (300 MHz, CDCl3) δ = 8.02 (d, J = 7.7 Hz, 1H), 7.78 (d, J = 7.5 Hz, 1H), 7.40-7.19 (m, 3H), 7.10 (s, 1H), 6.23 (s, 2H), 5.51 (s, 1H), 3.61 (s, 3H), 2.23 - 1.97 (m, 4H), 0.96 (dq, J = 6.9, 2.1 Hz, 1H), 0.82 (td, J = 7.2, 6.3, 3.5 Hz, 2H), 0.68 (dt, J = 8.6, 4.2 Hz, 1H).
HRMS (IE) m/z calculated C20H20N2O3S [M+]: 368.1195, found: 368.1183.

Example D3: Methyl 5-acetyl-2-amino-4-(5-fluorobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method D yielded the title compound as a yellow solid (0.075 g, 44%).
1H-NMR (300 MHz, CDCl3) δ = 7.75 - 7.67 (m, 2H), 7.48 (s, 1H), 7.19 (s, 1H), 7.05 (t, J = 7.7 Hz, 1H), 6.27 (s, 2H), 5.29 (s, 1H), 3.66 (s, 3H), 2.20 (s, 3H), 2.17 (s, 3H). 19F-NMR (282 MHz, CDCl3) δ = -119.10 (s).
HRMS (IE) m/z calculated C18H17FN2O3S [M+]: 360.0944, found: 360.0943.

Example D4: Methyl 5-acetyl-2-amino-4-(7-bromobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method D in methanol yielded the title compound as a yellow solid (0.031 g, 24%).
1H-NMR (300 MHz, CDCl3) δ = 8.82 (s, 1H), 8.06 (d, J = 8.0 Hz, 1H), 7.54 (d, J = 7.5 Hz, 1H), 7.39 - 7.27 (m, 2H), 6.61 (bs, 2H), 5.22 (s, 1H), 3.45 (s, 3H), 2.27 (s, 3H), 2.05 (s, 3H).
HRMS (IE) m/z calculated C18H17N2O3SBr [M+]: 420.0143, found: 420.0130.
HPLC (98.4%): Rt 16.46 min.

Example D5: Methyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method D in methanol yielded the title compound as a yellow solid (0.075 g, 64%).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.35 (d, J = 8.2 Hz, 1H), 7.66 (d, J = 7.3 Hz, 1H), 7.46 (t, J = 7.8 Hz, 1H), 7.27 (s, 1H), 6.33 (bs, 1H), 6.03 (bs, 2H), 5.39 (s, 1H), 3.61 (s, 3H), 2.35 (s, 3H), 2.14 (s, 3H).
HRMS (IE) m/z calculated C19H18N3O3S [M+]: 368.1063, found: 368.1064.
HPLC (98.4%): Rt 20.05 min.

Example D6: Cyclopentyl 5-acetyl-2-amino-4-(7-bromobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method D in methanol yielded the title compound as a yellow solid (0.070 g, 46 %).
1H-NMR (300 MHz, DMSO) δ (ppm): 8.83 (bs, 1H), 8.11 - 8.01 (m, 1H), 7.55 (d, J = 7.5 Hz, 1H), 7.39 - 7.26 (m, 2H), 6.65 (bs, 2H), 5.21 (s, 1H), 5.03 - 4.89 (m, 1H), 2.26 (s, 3H), 2.11 (s, 3H), 1.86 - 1.71 (m, 1H), 1.69 - 1.52 (m, 3H), 1.54 - 1.33 (m, 3H), 1.33 - 1.19 (m, 1H).
HRMS (IE) m/z calculated C22H23N2O3SBr [M+]: 474.0613, found: 474.0605.
HPLC (95.5%): Rt 13.81 min.

Example D7: Dimethyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D in methanol yielded the title compound as a yellow solid (0.045 g, 34 %).
1H-NMR (300 MHz, DMSO) δ (ppm): 8.33 (d, J = 8.0 Hz, 1H), 7.65 (d, J = 7.3 Hz, 1H), 7.43 (t, J = 8.0 Hz, 1H), 7.27 (s, 1H), 6.91 (bs, 1H), 6.20 (bs, 2H), 5.37 (s, 1H), 3.53 (s, 3H), 3.51 (s, 3H), 2.27 (s, 3H).

HRMS (IE) m/z calculated C19H17N3O4S [M+]: 383.0940, found: 383.0947.

HPLC (97.5%): Rt 14.58 min.

Example D8: Cyclopentyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method D in methanol yielded the title compound as a yellow solid (0.048 g, 31 %).
1H-NMR (300 MHz, DMSO) δ (ppm): 8.35 - 8.20 (m, 1H), 7.57 (d, J = 7.8 Hz, 1H), 7.35 (t, J = 7.8 Hz, 1H), 7.18 (bs, 2H), 6.16 (bs, 2H), 5.30 (s, 1H), 5.11 - 4.95 (m, 1H), 2.17 (s, 3H), 2.12 (s, 3H), 1.86 - 1.73 (m, 1H), 1.71 - 1.51 (m, 3H), 1.52 - 1.36 (m, 3H), 1.35 - 1.24 (m, 1H).
HRMS (IE) m/z calculated C23H23N3O3S [M+]: 421.1460, found: 421.1453.
HPLC (98.4%): Rt 17.10 min.

Example D9: Dimethyl 2,6-diamino-4-(benzo[b]thiophen-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate

Method D using benzo[b]thiophene-3-carbaldehyde and methyl 3-amino-3-iminopropanoate (2 eq) yielded the title compound as a yellow solid (0.115 g, 53 %). 1H-NMR (300 MHz, CDCl3) δ = 8.13-7.90 (m, 2H), 7.86 (d, J = 8.0 Hz, 1H), 7.58 - 7.34 (m, 3H), 7.19 (s, 1H), 6.98 (s, 1H), 6.54 (s, 1H), 4.74 (s, 1H), 3.72 (s, 3H), 3.60 (s, 1H), 3.37 (s, 3H).

Example D10: Cyclopropylmethyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method D using morpholine (1.2 eq.) in isopropanol yielded the title compound as a yellow solid (0.038 g, 51 %).
1H-NMR (300 MHz, CDCl3) δ = 8.43 (d, J = 8.3 Hz, 1H), 7.66 (d, J = 7.3 Hz, 1H), 7.43 (t, J = 7.8 Hz, 1H), 7.30 (s, 1H), 6.51 (bs, 1H), 6.08 (bs, 2H), 5.43 (s, 1H), 3.83 (d, J = 7.3 Hz, 2H), 2.33 (s, 3H), 2.17 (s, 3H), 1.10 - 0.95 (m, 1H), 0.60 - 0.40 (m, 2H), 0.27 - 0.03 (m, 2H).

Example D11: 4,4-difluorocyclohexyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method D using morpholine (1.2 eq.) in isopropanol yielded the title compound as a yellow solid (0.053 g, 30 %).
1H-NMR (300 MHz, CDCl3) δ = 8.35 (d, J = 8.2 Hz, 1H), 7.67 (d, J = 7.3 Hz, 1H), 7.45 (t, J = 7.8 Hz, 1H), 7.28 (s, 1H), 6.05 (bs, 2H), 5.42 (s, 1H), 4.82 (bs, 1H), 2.32 (s, 3H), 2.20 (s, 3H), 2.02 - 1.80 (m, 3H), 1.76 - 1.58 (m, 5H), 1.53 - 1.38 (m, 1H). 19F-NMR (282 MHz, CDCl3) δ (ppm): -97.60 (CF), -98.46 (CF).

Example D12: Methyl 2-amino-5-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method D using morpholine (1.2 eq.) in isopropanol yielded the title compound as a yellow solid (0.022 g, 15 %).
1H-NMR (300 MHz, CDCl3) δ = 8.24 - 8.16 (m, 1H), 7.74 - 7.67 (m, 1H), 7.47 (t, J = 7.8 Hz, 1H), 7.31 (s, 1H), 6.89 (bs, 1H), 6.26 (bs, 2H), 5.03 (s, 1H), 3.49 (s, 3H), 2.04 (s, 3H).

Example D13: 4-fluorobenzyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method D using morpholine (1.2 eq.) in isopropanol yielded the title compound as a yellow solid (0.074 g, 43 %).
1H-NMR (300 MHz, CDCl3) δ = 8.14 (d, J = 7.9 Hz, 1H), 7.60 (d, J = 7.0 Hz, 1H), 7.20 (s, 2H), 7.13 - 7.02 (m, 2H), 6.95 (t, J = 8.2 Hz, 2H), 6.48 (bs, 1H), 6.12 (bs, 2H), 5.37 (s, 1H), 4.98 (s, 2H), 2.31 (s, 3H), 2.12 (s, 3H). 19F-NMR (282 MHz, CDCl3) δ (ppm): - 113.91 (CF).

Example D14: Methyl 5-acetyl-2-amino-4-(5-fluorothieno[2,3-b]pyridin-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method D in methanol yielded the title compound as a yellow solid (0.012 g, 67 %).
1H-NMR (300 MHz, CDCl3) δ = 8.90 (s, 1H), 8.55 (d, J = 2.6 Hz, 1H), 8.16 (dd, J = 10.5, 2.6 Hz, 1H), 7.48 (s, 1H), 6.65 (bs, 2H), 5.20 (s, 1H), 3.48 (s, 3H), 2.30 (s, 3H), 2.11 (s, 3H). 19F-NMR (282 MHz, CDCl3) δ (ppm): -129.28 (d, J = 10.4 Hz).

Example D14 was further modified to render Example D15:

Example D15: Methyl 2-acetamido-5-acetyl-4-(5-fluorothieno[2,3-b]pyridin-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

A mixture of methyl 5-acetyl-2-amino-4-(5-fluorothieno[2,3-b]pyridin-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate (0.025 g, 0.07 mmol) and acetyl chloride (0.005 mL, 0.07 mmol) in pyridine (1 mL) was stirred at 75 °C for 3 hours. The mixture was allowed to cool to RT and the solvent was removed under reduced pressure. The residue was purified by column chromatography (DCM:MeOH 2%) affording a yellow solid (0.017 g, 61%).
1H-NMR (300 MHz, CDCl3) δ = 11.71 (bs, 1H), 10.50 (s, 1H), 8.43 (d, J = 2.4 Hz, 1H), 8.02 (dd, J = 9.8, 2.7 Hz, 1H), 7.34 (s, 1H), 5.33 (s, 1H), 3.66 (s, 3H), 2.39 (s, 3H), 2.21 (s, 3H), 2.18 (s, 3H). 19F-NMR (282 MHz, CDCl3) δ (ppm): -133.66 (d, J = 9.5 Hz).

Example D16: Cyclopentylmethyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method D using morpholine (1.2 eq.) in isopropanol yielded the title compound as a yellow solid (0.066 g, 51 %).
1H-NMR (300 MHz, CDCl3) δ = 8.36 (d, J = 8.2 Hz, 1H), 7.65 (d, J = 7.4 Hz, 1H), 7.43 (t, J = 7.8 Hz, 1H), 7.26 (s, 1H), 6.25 (bs, 1H), 6.02 (bs, 2H), 5.42 (s, 1H), 3.90 (dd, J = 7.2, 1.8 Hz, 2H), 2.32 (s, 3H), 2.18 (s, 3H), 1.58 (d, J = 34.3 Hz, 7H), 1.28 - 1.04 (m, 2H).

Example D17: 3-(cyclopropylmethyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine (1.2 eq.) in isopropanol yielded the title compound as a yellow solid (0.070 g, 47 %).
1H-NMR (300 MHz, CDCl3) δ = 8.36 (d, J = 8.7 Hz, 1H), 7.64 (d, J = 7.1 Hz, 1H), 7.47 - 7.37 (m, 1H), 7.32 (s, 1H), 5.99 (bs, 2H), 5.87 (bs, 1H), 5.41 (s, 1H), 3.71 (d, J = 7.2 Hz, 2H), 3.52 (s, 3H), 2.35 (s, 3H), 0.91 - 0.75 (m, 1H), 0.44 - 0.24 (m, 2H), 0.13 - - 0.10 (m, 2H).

Example D18: Dimethyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-cyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D in methanol yielded the title compound as a yellow solid (0.054 g, 41 %).
1H-NMR (300 MHz, CDCl3) δ = 8.32 (d, J = 8.2 Hz, 1H), 7.65 (d, J = 7.3 Hz, 1H), 7.44 (t, J = 7.8 Hz, 1H), 7.24 (s, 1H), 6.18 (bs, 2H), 6.15 (bs, 1H), 5.39 (s, 1H), 3.56 (s, 3H), 3.52 (s, 3H), 2.81 - 2.64 (m, 1H), 1.01 - 0.84 (m, 2H), 0.83 - 0.60 (m, 2H).

Example D19: 3-(cyclopropylmethyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-cyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D yielded the title compound as a yellow solid (0.017 g, 12 %).
1H-NMR (300 MHz, CDCl3) δ = 8.37 (d, J = 8.2 Hz, 1H), 7.64 (d, J = 7.3 Hz, 1H), 7.42 (t, J = 7.8 Hz, 1H), 7.28 (s, 1H), 6.15 (bs, 2H), 6.02 (bs, 1H), 5.42 (s, 1H), 3.72 (d, J = 7.2 Hz, 2H), 3.55 (s, 3H), 2.78 - 2.62 (m, 1H), 1.01 - 0.77 (m, 3H), 0.79 - 0.62 (m, 2H), 0.47 - 0.24 (m, 2H), 0.12 - -0.09 (m, 2H).

Example D20: dimethyl 2,6-diamino-4-(7-cyanobenzo[b]thiophen-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate

Method D in methanol yielded the title compound as a yellow solid (0.135 g, 66 %).
1H-NMR (300 MHz, CDCl3) δ = 8.14 (d, J = 7.9 Hz, 1H), 7.98 (d, J = 7.2 Hz, 1H), 7.69 (t, J = 7.9 Hz, 1H), 7.47 (bs, 1H), 7.21 (s, 1H), 7.16 (bs, 1H), 6.60 (bs, 2H), 4.74 (s, 1H), 3.70 (s, 3H), 3.56 (s, 1H), 3.35 (s, 3H).

Example D21: 5-(cyclopropylmethyl) 3-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-cyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine in isopropanol yielded the title compound as a yellow solid (0.040 g, 31 %).
1H-NMR (300 MHz, CDCl3) δ = 8.35 (d, J = 8.2 Hz, 1H), 7.64 (d, J = 7.2 Hz, 1H), 7.42 (t, J = 7.7 Hz, 1H), 7.26 (s, 1H), 6.26 (bs, 3H), 5.41 (s, 1H), 3.77 (d, J = 7.2 Hz, 2H), 3.52 (s, 3H), 2.84 - 2.66 (m, 1H), 0.97 - 0.80 (m, 3H), 0.81 - 0.61 (m, 2H), 0.48 - 0.25 (m, 2H), 0.15 - -0.08 (m, 2H).

Example D22: 3-(4-fluorobenzyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine in isopropanol yielded the title compound as a yellow solid (0.035 g, 25 %).
1H-NMR (300 MHz, CDCl3) δ = 8.11 (d, J = 8.3 Hz, 1H), 7.59 (d, J = 7.3 Hz, 1H), 7.30-7.15 (m, 2H), 6.97 - 6.77 (m, 4H), 6.68 (s, 1H), 6.22 (bs, 2H), 5.34 (s, 1H), 5.04 - 4.75 (m, 2H), 3.50 (s, 3H), 2.26 (s, 3H). 19F-NMR (282 MHz, CDCl3) δ (ppm): -114.23 (CF).

Example D23: 3-(4-fluorobenzyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-cyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine in isopropanol yielded the title compound as a yellow solid (0.035 g, 25 %).
1H-NMR (300 MHz, CDCl3) δ = 8.10 (d, J = 8.2 Hz, 1H), 7.60 (d, J = 7.3 Hz, 1H), 7.25-7.13 (m, 2H), 6.87 (q, J = 8.7, 7.6 Hz, 4H), 6.29 (s, 2H), 6.20 (s, 1H), 5.36 (s, 1H), 5.00 - 4.77 (m, 2H), 3.52 (s, 3H), 2.83 - 2.53 (m, 1H), 0.99 - 0.82 (m, 2H), 0.79 - 0.62 (m, 2H). 19F-NMR (282 MHz, CDCl3) δ (ppm): -114.26 (CF).

Example D24: cyclopropylmethyl 2-amino-5-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method D using piperidine in dioxane yielded the title compound as a yellow solid (0.035 g, 23 %).
1H-NMR (300 MHz, CDCl3) δ = 8.20 (d, J = 8.3 Hz, 1H), 7.69 (d, J = 7.4 Hz, 1H), 7.45 (t, J = 7.8 Hz, 1H), 7.35 (s, 1H), 7.04 (bs, 1H), 6.27 (bs, 2H), 5.08 (s, 1H), 3.69 (dt, J = 7.2, 3.8 Hz, 2H), 2.02 (s, 3H), 0.83 - 0.64 (m, 1H), 0.35 - 0.11 (m, 2H), 0.06 - -0.29 (m, 2H).
HRMS (APCI) m/z calculated C21H19N4O2S [M+1]: 391.1223, found: 391.1223.

Example D25: 4-Fluorobenzyl 2-amino-5-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method D using morpholine in dioxane yielded the title compound as a yellow solid (0.010 g, 8 %).
1H-NMR (300 MHz, CDCl3) δ = 8.08 (d, J = 8.2 Hz, 1H), 7.69 (d, J = 7.1 Hz, 1H), 7.38 (t, J = 7.8 Hz, 1H), 7.28 (s, 1H), 6.79 (s, 2H), 6.77 (s, 2H), 6.46 (bs, 1H), 6.28 (s, 2H), 5.05 (s, 1H), 4.98 - 4.75 (m, 2H), 2.09 (s, 3H). 19F-NMR (282 MHz, CDCl3) δ (ppm): - 114.12 (CF).
HRMS (APCI) m/z calculated C24H18N4O2S [M+1]: 445.1129, found: 445.1126.

Example D26: Cyclopropylmethyl 6-amino-5-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-1,4-dihydropyridine-3-carboxylate

Method D run at room temperature overnight yielded the title compound as a yellow solid (0.060 g, 51 %).
1H-NMR (300 MHz, DMSO-d6) δ = 8.20 (d, J = 8.3 Hz, 1H), 7.95 (d, J = 7.3 Hz, 1H), 7.76 (bs, 1H), 7.61 (t, J = 7.8 Hz, 1H), 6.77 (s, 1H), 5.51 (bs, 2H), 5.21 (s, 1H), 4.60 - 4.35 (m, 1H), 3.56 (d, J = 7.2 Hz, 2H), 1.23 - 1.10 (m, 1H), 0.81 - 0.64 (m, 1H),0.64 - 0.47 (m, 1H), 0.39 - 0.29 (m, 2H), 0.27 - 0.12 (m, 2H), 0.04 - -0.20 (m, 2H).
HRMS (APCI) m/z calculated C23H21N4O2S [M+1]: 417.1380, found: 417.1382.

Example D27: 3-Cyclopentyl 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.082 g, 51 %).
1H-NMR (300 MHz, CDCl3) δ = 8.30 (d, J = 8.7 Hz, 1H), 7.65 (d, J = 7.3 Hz, 1H), 7.43 (t, J = 7.8 Hz, 1H), 7.30 (s, 1H), 6.07 (s, 2H), 6.03 (s, 1H), 5.32 (s, 1H), 5.07 - 4.92 (m, 1H), 3.54 (s, 3H), 2.32 (s, 3H), 1.87 - 1.74 (m, 1H), 1.71 - 1.57 (m, 2H), 1.54 - 1.40 (m, 3H), 1.39 - 1.29 (m, 1H), 1.24 - 1.11 (m, 1H).
HRMS (APCI) m/z calculated C23H24N3O4S [M+1]: 438.1482, found: 438.1481.

Example D28: Cyclopropylmethyl 5-acetyl-2-amino-4-(6-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.062 g, 45 %).
1H-NMR (300 MHz, CDCl3) δ = 8.93 (bs, 1H), 8.53 (s, 1H), 8.25 (d, J = 8.4 Hz, 1H), 7.75 (d, J = 8.4 Hz, 1H), 7.59 (s, 1H), 6.69 (bs, 2H), 5.32 (s, 1H), 3.79 - 3.62 (m, 2H), 2.30 (s, 3H), 2.09 (s, 3H), 1.06 - 0.87 (m, 1H), 0.40 (d, J = 8.0 Hz, 2H), 0.20 - 0.02 (m, 2H).
HRMS (APCI) m/z calculated C22H22N3O3S [M+1]: 408.1376, found: 408.1378.

Example D29: Cyclohexyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.066 g, 43 %).
1H-NMR (300 MHz, CDCl3) δ = 8.37 (d, J = 8.0 Hz, 1H), 7.66 (d, J = 7.1 Hz, 1H), 7.43 (t, J = 7.8 Hz, 1H), 7.27 (s, 1H), 6.25 (bs, 1H), 6.03 (bs, 2H), 5.40 (s, 1H), 4.77 - 4.56 (m, 1H), 2.32 (s, 3H), 2.17 (s, 3H), 1.99 - 1.86 (m, 1H), 1.78 - 1.67 (m, 1H), 1.63 - 1.49 (m, 3H), 1.39 - 1.07 (m, 5H).
HRMS (APCI) m/z calculated C24H26N3O3S [M+1]: 436.1689, found: 436.1690.

Example D30: Cyclohexylmethyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.077 g, 46 %).
1H-NMR (300 MHz, CDCl3) δ = 8.37 (d, J = 8.1 Hz, 1H), 7.66 (d, J = 7.3 Hz, 1H), 7.45 (t, J = 7.8 Hz, 1H), 7.28 (s, 1H), 6.38 (bs, 1H), 6.06 (bs, 2H), 5.41 (s, 1H), 3.81 (d, J = 6.6 Hz, 2H), 2.31 (s, 3H), 2.18 (s, 3H), 1.69 - 1.53 (m, 4H), 1.51 - 1.37 (m, 2H), 1.16 - 0.96 (m, 3H), 0.87 - 0.73 (m, 2H).
HRMS (APCI) m/z calculated C25H28N3O3S [M+1]: 450.1846, found: 450.1849.

Example D31: Cyclopropylmethyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-5-(cyclopropanecarbonyl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.120 g, 40 %).
1H-NMR (300 MHz, CDCl3) δ = 8.38 (d, J = 8.2 Hz, 1H), 7.66 (d, J = 7.3 Hz, 1H), 7.42 (t, J = 7.8 Hz, 1H), 7.32 (s, 1H), 6.54 (bs, 1H), 6.14 (bs, 2H), 5.56 (s, 1H), 3.82 (d, J = 7.3 Hz, 2H), 2.25 (s, 3H), 2.13 - 1.98 (m, 1H), 1.04 - 0.94 (m, 2H), 0.91 - 0.69 (m, 3H), 0.50 - 0.38 (m, 2H), 0.21 - 0.05 (m, 2H).
HRMS (APCI) m/z calculated C24H24N3O3S [M+1]: 434.1533, found: 421.1537.

Example D32: 3-Fluorobenzyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.058 g, 34 %).
1H-NMR (300 MHz, CDCl3) δ = 8.17 (d, J = 8.5 Hz, 1H), 7.60 (d, J = 7.3 Hz, 1H), 7.23 (s, 3H), 6.96 (t, J = 8.5 Hz, 1H), 6.86 (d, J = 7.3 Hz, 1H), 6.74 (d, J = 11.4 Hz, 1H), 6.41 (bs, 1H), 6.12 (bs, 2H), 5.39 (s, 1H), 5.06 - 4.92 (m, 2H), 2.32 (s, 3H), 2.13 (s, 3H).
19F-NMR (282 MHz, CDCl3) δ (ppm): -112.76 (s, CF).
HRMS (APCI) m/z calculated C25H21N3O3SF [M+1]: 462.1282, found: 462.1278.

Example D33: 3-(Cyclobutylmethyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.040 g, 33 %).
1H-NMR (300 MHz, CDCl3) δ = 8.31 (d, J = 8.1 Hz, 1H), 7.66 (d, J = 7.2 Hz, 1H), 7.44 (t, J = 7.8 Hz, 1H), 7.29 (s, 1H), 6.82 (s, 1H), 6.22 (bs, 2H), 5.35 (s, 1H), 3.89 (t, J = 7.2 Hz, 2H), 3.54 (s, 3H), 2.41 - 2.29 (m, 1H), 2.26 (s, 3H), 1.93 - 1.63 (m, 4H), 1.58 - 1.27 (m, 2H).
HRMS (APCI) m/z calculated C23H24N3O4S [M+]:438.1482, found: 438.1483.

Example D34: 3-((3,3-Difluorocyclobutyl)methyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.035 g, 22 %).
1H-NMR (300 MHz, CDCl3) δ = 8.28 (d, J = 8.2 Hz, 1H), 7.67 (d, J = 7.2 Hz, 1H), 7.46 (t, J = 7.8 Hz, 1H), 7.31 (s, 1H), 6.19 (s, 1H), 6.14 (bs, 2H), 5.35 (s, 1H), 4.00 - 3.81 (m, 2H), 3.55 (s, 3H), 2.35 (m, 1H), 2.32 (s, 3H), 2.26 - 1.93 (m, 4H).
19F-NMR (282 MHz, CDCl3) δ (ppm): -83.87 (d, J = 193.0 Hz), -93.31 (d, J = 193.0 Hz).
HRMS (APCI) m/z calculated C23H22N3O4SF2 [M+]:474.1294, found: 474.1295.

Example D35: Cyclopropylmethyl 2-amino-5-carbamoyl-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.011 g, 11 %).
1H-NMR (300 MHz, CDCl3) δ = 8.28 (d, J = 8.2 Hz, 1H), 7.66 (d, J = 7.3 Hz, 1H), 7.46-7.33 (m, 2H), 6.93 (bs, 1H), 6.28 (bs, 2H), 5.37 (bs, 2H), 5.21 (s, 1H), 3.76 (d, J = 7.2 Hz, 2H), 2.16 (s, 3H), 0.95 - 0.85 (m, 1H), 0.53 - 0.27 (m, 2H), 0.23 - -0.06 (m, 2H). HRMS (APCI) m/z calculated C21H21N4O3S [M+1]: 409.1329, found: 409.1324.

Example D36: 3-((2,2-Difluorocyclopropyl)methyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.065 g, 40 %).
1H-NMR (300 MHz, CDCl3) δ = 8.31 (dd, J = 7.7, 2.8 Hz, 1H), 7.65 (d, J = 7.0 Hz, 1H), 7.52 - 7.38 (m, 1H), 7.31 (d, J = 3.3 Hz, 1H), 6.07 (bs, 3H), 5.37 (s, 1H), 4.16 - 3.95 (m, 1H), 3.91 - 3.71 (m, 1H), 3.52 (s, 3H), 2.35 (s, 3H), 1.71 - 1.60 (m, 1H), 1.29 - 1.16 (m, 1H), 0.90 (s, 1H). 19F-NMR (282 MHz, CDCl3) δ (ppm): -129.21 (ddt, J = 160.2, 70.2, 10.9 Hz), -143.62 (ddd, J = 160.2, 51.3, 10.9 Hz).
HRMS (APCI) m/z calculated C22H20N3O4SF2 [M+]: 460.1137, found: 460.1137.

Example D37: 5-Cyclopropyl 3-(cyclopropylmethyl) 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.008 g, 5 %).
1H-NMR (300 MHz, CDCl3) δ = 8.32 (d, J = 8.2 Hz, 1H), 7.65 (d, J = 7.2 Hz, 1H), 7.47 - 7.36 (m, 1H), 7.28 (s, 1H), 6.35 (bs, 1H), 6.11 (bs, 2H), 5.31 (s, 1H), 4.03 - 3.89 (m, 1H), 3.71 (d, J = 7.2 Hz, 2H), 2.33 (s, 3H), 1.25 (s, 1H), 1.00 - 0.69 (m, 2H), 0.60 - 0.28 (m, 4H), 0.22 - -0.03 (m, 2H).
HRMS (APCI) m/z calculated C24H24N3O4S [M+1]: 450.1482, found: 450.1481.

Example D38: 3-((2,2-Difluorocyclopropyl)methyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-cyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.004 g, 26 %).
1H-NMR (300 MHz, CDCl3) δ = 8.32 (d, J = 8.3 Hz, 1H), 7.65 (d, J = 7.2 Hz, 1H), 7.44 (t, J = 7.8 Hz, 1H), 7.27 (s, 1H), 6.30 (bs, 3H), 5.38 (s, 1H), 4.15 - 3.95 (m, 1H), 3.91 - 3.73 (m, 1H), 3.55 (s, 3H), 2.77 - 2.62 (m, 1H), 1.84 - 1.40 (m, 1H), 1.28 - 1.17 (m, 2H), 1.03 - 0.52 (m, 4H). 19F-NMR (282 MHz, CDCl3) δ (ppm): -129.11 (ddt, J = 160.0, 62.6, 11.7 Hz), -143.52 (ddd, J = 160.0, 35.4, 11.7 Hz).
HRMS (APCI) m/z calculated C24H22N3O4SF2 [M+1]: 486.1294, found: 486.1292.

Example D39: 3-(Cyclopropylmethyl) 5-methyl 2-amino-4-(7-cyano-5-fluorobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.020 g, 28 %).
1H-NMR (300 MHz, CDCl3) δ = 8.05 (dd, J = 10.0, 2.3 Hz, 1H), 7.46 - 7.36 (m, 2H), 6.14 (s, 1H), 6.08 (bs, 2H), 5.32 (s, 1H), 3.73 (d, J = 7.3 Hz, 2H), 3.55 (s, 3H), 2.35 (s, 3H), 0.94 - 0.78 (m, 1H), 0.53 - 0.25 (m, 2H), 0.17 - -0.11 (m, 2H). 19F-NMR (282 MHz, CDCl3) δ (ppm): -117.83 (d, J = 7.7 Hz).
HRMS (APCI) m/z calculated C22H21N3O4SF [M+1]: 442.1231, found: 442.1231.

Example D40: 3-(cyclopropylmethyl) 5-methyl 2-amino-4-(7-cyano-4-fluorobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.015 g, 16 %).
1H-NMR (300 MHz, CDCl3) δ = 7.62 (dd, J = 8.2, 4.0 Hz, 1H), 7.33 (s, 1H), 7.09 (dd, J = 11.0, 8.2 Hz, 1H), 6.15 - 6.01 (m, 3H), 5.66 (d, J = 4.0 Hz, 1H), 3.76 - 3.57 (m, 2H), 3.48 (s, 3H), 2.32 (s, 3H), 0.91 - 0.59 (m, 1H), 0.39 - 0.02 (m, 2H), 0.02 - -0.25 (m, 2H). 19F-NMR (282 MHz, CDCl3) δ (ppm): -104.35 (d, J = 11.0 Hz).
HRMS (APCI) m/z calculated C22H21N3O4SF [M+1]: 442.1231, found: 442.1229.

Example D41: 3-Isopropyl 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.045 g, 26 %).
1H-NMR (300 MHz, CDCl3) δ = 8.34 (d, J = 8.2 Hz, 1H), 7.65 (d, J = 7.2 Hz, 1H), 7.43 (t, J = 7.8 Hz, 1H), 7.29 (s, 1H), 6.91 (bs, 1H), 6.25 (bs, 2H), 5.34 (s, 1H), 4.95 - 4.76 (m, 1H), 3.53 (s, 3H), 2.26 (s, 3H), 1.18 (d, J = 6.1 Hz, 3H), 0.70 (d, J = 6.1 Hz, 3H).
HRMS (APCI) m/z calculated C21H22N3O4S [M+1]: 412.1326, found: 412.1335.

Example D42: 3-((2,2-Difluoro-3,3-dimethylcyclopropyl)methyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.052 g, 31 %).
1H-NMR (300 MHz, CDCl3) δ = 8.32 (ddd, J = 8.3, 5.3, 1.0 Hz, 1H), 7.65 (d, J = 7.4 Hz, 1H), 7.50 - 7.39 (m, 1H), 7.31 (s, 1H), 6.12 (d, J = 3.4 Hz, 1H), 6.08 (d, J = 2.2 Hz, 2H), 5.37 (s, 1H), 4.16 - 4.02 (m, 1H), 3.97 - 3.81 (m, 1H), 3.55 (d, J = 1.5 Hz, 3H), 2.33 (d, J = 2.9 Hz, 3H), 1.40 - 1.21 (m, 1H), 1.15 (dd, J = 2.4, 1.5 Hz, 2H), 1.01 - 0.92 (m, 3H), 0.90 (dd, J = 2.9, 1.4 Hz, 1H). 19F-NMR (282 MHz, CDCl3) δ (ppm): -137.08 (ddd, J = 156.1, 65.1, 13.6 Hz), -148.04 (dd, J = 155.7, 19.2 Hz)
HRMS (APCI) m/z calculated C24H24N3O4SF2 [M+1]: 488.1450, found: 488.1468.

Example D43: 5-Methyl 3-neopentyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.061 g, 40 %).
1H-NMR (300 MHz, CDCl3) δ = 8.34 (d, J = 8.2 Hz, 1H), 7.63 (d, J = 7.1 Hz, 1H), 7.45-7.36 (m, 1H), 7.32 (s, 1H), 6.10 (bs, 3H), 5.44 (s, 1H), 3.80 - 3.53 (m, 5H), 2.30 (s, 3H), 0.74 (s, 9H).
HRMS (APCI) m/z calculated C23H26N3O4S [M+1]: 440.1639, found: 440.1638.

Example D44: 3-(Cyclopropylmethyl) 5-methyl 2-amino-4-(7-cyanothieno[3,2-b]pyridin-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.048 g, 42 %).
1H-NMR (300 MHz, CDCl3) δ = 8.77 (d, J = 4.7 Hz, 1H), 7.64 (s, 1H), 7.42 (d, J = 4.7 Hz, 1H), 6.34 (s, 1H), 6.13 (s, 2H), 5.54 (s, 1H), 3.83 - 3.62 (m, 2H), 3.56 (s, 3H), 2.26 (s, 3H), 0.92 - 0.75 (m, 1H), 0.39 - 0.17 (m, 2H), 0.10 - -0.13 (m, 2H).
HRMS (APCI) m/z calculated C21H21N4O4S [M+1]: 425.1278, found: 425.1280.

Example D45: 5-Methyl 3-neopentyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-cyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.063 g, 35 %). 1H-NMR (300 MHz, CDCl3) δ = 8.33 (dd, J = 8.3, 1.0 Hz, 1H), 7.64 (dd, J = 7.6, 0.8 Hz, 1H), 7.41 (dd, J = 8.3, 7.4 Hz, 1H), 7.28 (s, 1H), 6.13 (bs, 2H), 5.91 (bs, 1H), 5.46 (s, 1H), 3.75 - 3.56 (m, 5H), 2.68 - 2.51 (m, 1H), 0.94 - 0.84 (m, 2H), 0.75 (s, 9H), 0.72 - 0.64 (m, 2H).
HRMS (APCI) m/z calculated C25H28N3O4S [M+1]: 466.1795, found: 466.1798.

Example D46: Bis(cyclopropylmethyl) 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.120 g, 56 %).
1H-NMR (300 MHz, CDCl3) δ = 8.40 (d, J = 7.3 Hz, 1H), 7.63 (d, J = 8.4 Hz, 1H), 7.39 (dd, J = 8.2, 7.4 Hz, 1H), 7.34 (s, 1H), 6.02 (bs, 3H), 5.44 (s, 1H), 3.83 - 3.64 (m, 4H), 2.36 (s, 3H), 0.97 - 0.75 (m, 2H), 0.51 - 0.23 (m, 4H), 0.16 - -0.07 (m, 4H).
HRMS (APCI) m/z calculated C25H26N3O4S [M+1]: 464.1639, found: 464.11638.

Example D47: 3-(Cyclopropylmethyl) 5-(prop-2-yn-1-yl) 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.112 g, 52 %).
1H-NMR (300 MHz, CDCl3) δ = 8.39 (dd, J = 8.3, 1.0 Hz, 1H), 7.64 (dd, J = 7.1, 1.2 Hz, 1H), 7.41 (dd, J = 8.2, 7.4 Hz, 1H), 7.34 (s, 1H), 6.02 (bs, 3H), 5.44 (s, 1H), 4.63 - 4.42 (m, 2H), 3.71 (d, J = 7.3 Hz, 2H), 2.46 - 2.26 (m, 4H), 0.91 - 0.73 (m, 1H), 0.49 - 0.20 (m, 2H), 0.12 - -0.14 (m, 2H).
HRMS (APCI) m/z calculated C24H22N3O4S [M+1]: 448.1326, found: 448.1327.

Example D48: 3-(Cyclopropylmethyl) 5-methyl 2-amino-4-(5,7-dicyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.005 g, 8 %).
1H-NMR (300 MHz, CDCl3) δ = 8.64 (s, 1H), 7.85 (s, 1H), 7.49 (s, 1H), 6.07 (bs, 2H), 5.97 (bs, 1H), 5.40 (s, 1H), 3.72 (d, J = 7.3 Hz, 2H), 3.55 (s, 3H), 2.37 (s, 3H), 0.87-0.82 (m, 1H), 0.58 - 0.29 (m, 2H), 0.16 - -0.11 (m, 2H).
HRMS (APCI) m/z calculated C23H21N4O4S [M+1]: 449.1278, found: 449.1277.

Example D49: 5-(But-2-yn-1-yl) 3-(cyclopropylmethyl) 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.007 g, 4 %).
1H-NMR (300 MHz, CDCl3) δ = 8.39 (dd, J = 8.3, 1.0 Hz, 1H), 7.64 (dd, J = 7.1, 1.2 Hz, 1H), 7.41 (dd, J = 8.2, 7.4 Hz, 1H), 7.34 (s, 1H), 6.02 (bs, 3H), 5.44 (s, 1H), 4.63 - 4.42 (m, 2H), 3.71 (d, J = 7.3 Hz, 2H), 2.46 - 2.26 (m, 4H), 0.91 - 0.73 (m, 1H), 0.49 - 0.20 (m, 2H), 0.12 - -0.14 (m, 2H).
HRMS (APCI) m/z calculated C25H24N3O4S [M+1]: 462.1482, found: 462.1480.

Example D50: 5-Methyl 3-(2,2,2-trifluoroethyl) 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

The title compound was prepared using a variation of Method D: A mixture of methyl (Z)-2-((7-cyanobenzo[b]thiophen-3-yl)methylene)-3-oxobutanoate (0.123 g, 0.43 mmol), 2,2,2-trifluoroethyl 3-amino-3-iminopropanoate (0.115 g, 0.43 mmol), ammonium acetate (0.033 g, 0.43 mmol) in trifluoroethanol (3 mL) was stirred at room temperature overnight and then concentrated. The residue was purified by column chromatography (1:1 hexane: ethyl acetate) affording a yellow solid (0.019 g, 10%).
1H-NMR (300 MHz, CDCl3) δ = 8.30 (d, J = 8.3 Hz, 1H), 7.68 (d, J = 7.2 Hz, 1H), 7.46 (t, J = 7.7 Hz, 1H), 7.34 (s, 1H), 6.55 (bs, 1H), 6.24 (bs, 2H), 5.41 (s, 1H), 4.54 - 4.38 (m, 1H), 4.23 - 4.07 (m, 1H), 3.57 (s, 3H), 2.35 (s, 3H). 19F-NMR (282 MHz, CDCl3) δ (ppm): -73.93 (t, J = 9.8 Hz).
HRMS (APCI) m/z calculated C20H17N3O4SF3 [M+1]: 452.0886, found: 452.0885.

Example D51: 3-(Cyclopropylmethyl) 5-(2,2,2-trifluoroethyl) 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.0065 g, 7 %).
1H-NMR (300 MHz, CDCl3) δ = 8.34 (d, J = 8.3 Hz, 1H), 7.65 (d, J = 7.2 Hz, 1H), 7.44-7.38 (m, 1H), 7.34 (s, 1H), 6.16 (bs, 1H), 6.04 (bs, 2H), 5.42 (s, 1H), 4.41 (dq, J = 12.7, 8.5 Hz, 1H), 4.20 (dt, J = 12.7, 8.5 Hz, 1H), 3.73 (d, J = 7.3 Hz, 2H), 2.38 (s, 3H), 0.90 - 0.82 (m, 1H), 0.47 - 0.29 (m, 2H), 0.10 - -0.04 (m, 2H). 19F-NMR (282 MHz, CDCl3) δ
(ppm): -73.88 (t, J = 9.1 Hz).
HRMS (APCI) m/z calculated C23H21N3O4SF3 [M+1]: 492.1199, found: 492.1197.

Example D52: 3-(Cyclopropylmethyl) 5-methyl 2-amino-4-(6-chloro-7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.060 g, 39 %).
1H-NMR (300 MHz, CDCl3) δ = 8.27 (d, J = 8.8 Hz, 1H), 7.44 (d, J = 8.8 Hz, 1H), 7.29 (s, 1H), 6.14 - 5.94 (m, 3H), 5.35 (s, 1H), 3.76 - 3.67 (m, 2H), 3.54 (s, 3H), 2.34 (s, 3H), 0.93 - 0.75 (m, 1H), 0.48 - 0.24 (m, 2H), 0.12 - -0.09 (m, 2H)
HRMS (APCI) m/z calculated C22H21N3O4SCl [M+1]: 458.0936, found: 458.0938.

Example D53: 3-(2-Fluoro-2-methylpropyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.060 g, 39 %).
1H-NMR (300 MHz, CDCl3) δ = 8.34 (d, J = 9.0 Hz, 1H), 7.64 (d, J = 7.4 Hz, 1H), 7.47-7.37 (m, 1H), 7.32 (s, 1H), 6.63 (bs, 1H), 6.20 (bs, 2H), 5.43 (s, 1H), 4.12 - 3.79 (m, 2H), 3.56 (s, 3H), 2.30 (s, 3H), 1.27 - 1.03 (m, 6H). 19F-NMR (282 MHz, CDCl3) δ (ppm): (-142.28) - (-148.26) (m, CF).
HRMS (APCI) m/z calculated C22H23N3O4SF [M+1]: 444.1388, found: 444.1388.

Example D54: 3-(Cyclopropylmethyl) 5-methyl 2-amino-4-(7-cyano-6-(trifluoromethyl)benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.003 g, 3 %).
1H-NMR (300 MHz, CDCl3) δ = 8.09 (d, J = 8.4 Hz, 1H), 7.44 (d, J = 8.2 Hz, 1H), 7.35 (s, 1H), 6.03 - 5.91 (m, 3H), 5.24 (s, 1H), 3.84 (d, J = 7.0 Hz, 2H), 3.58 (s, 3H), 2.22 (s, 3H), 0.88 - 0.77 (m, 1H), 0.50 - 0.22 (m, 2H), 0.14 - -0.10 (m, 2H).

Example D55: 5-Methyl 3-prop-2-yn-1-yl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.005 g, 4 %).
1H-NMR (300 MHz, CDCl3) δ = 8.34 (d, 1H), 7.63 (d, 1H), 7.49 (t, 1H), 7.31 (s, 1H), 6.1 (bs, 3H), 5.44 (s, 1H), 4.44 - 4.26 (m, 2H), 3.59 (s, 3H), 2.42-2.24 (m, 4H).

Example D56: 3-(Cyclopropylmethyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-(trifluoromethyl)-1,4-dihydropyridine-3,5-dicarboxylate

Method D using morpholine at room temperature overnight yielded the title compound as a yellow solid (0.007 g, 5 %).
1H-NMR (300 MHz, CDCl3) δ = 8.33 (d, 1H), 7.65 (d, 1H), 7.44 - 7.35 (m, 1H), 7.31 (s, 1H), 6.09 (bs, 2H), 5.96 (bs, 1H), 5.48 (s, 1H), 3.76 (d, 2H), 2.38 (s, 3H), 0.98 - 0.81 (m, 1H), 0.46 - 0.26 (m, 2H), 0.14 - -0.11 (m, 2H).

Method E: Example E1: 4-(Benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-dicarbonitrile

A mixture of thianaphthene-3-carboxaldehyde (0.5 g, 3.08 mmol), 3-aminocrotonitrile (0.554 mL, 6.74 mmol) and acetic acid (0.076 mL, 3.08 mmol) in isopropyl alcohol (10 mL) was stirred at 100°C for 18 hours. The mixture was allowed to cool to RT and concentrated. The residue was basified with aq. sodium bicarbonate, and the resulting solid was filtered off and washed with cold water and ethyl ether. The desired product was obtained as a light-yellow solid (0.738 g, 82 %).
1H-NMR (400 MHz, DMSO-d6) δ = 2.06 (s, 6H), 5.00 (s, 1H), 7.36-7.46 (m, 1H), 7.65 (s, 1H), 7.89 (d, 1H), 8.03 (d, 1H), 9.67 (s, 1H).
HPLC-MS: Rt 3.878 min, m/z 292.0 (MH+).

The following examples were synthesized according to Method E:

Example E2: 4-(6-hydroxybenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-dicarbonitrile

1H-NMR (400 MHz, DMSO-d6) δ = 2.05 (s, 6H), 4.80 (s, 1H), 6.86 (dd, 1H), 7.13 (s, 1H), 7.24 (d, 1H), 7.61 (d, 1H), 9.64 (s, 2H).

HPLC-MS: Rt 3.285 min, m/z 308.0 (MH+).

Example E3: 4-(6-methoxybenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-dicarbonitrile

1H-NMR (400 MHz, DMSO-d6) δ = 2.06 (s, 6H), 3.81 (s, 3H), 4.84 (s, 1H), 7.00 (d, 1H), 7.20 (s, 1H), 7.50 (d, 1H), 7.71 (d, 1H), 9.69 (s, 1H).

HPLC-MS: Rt 3.962 min, m/z 322.0 (MH+).

Example E4: 2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3,5-dicarbonitrile

1H-NMR (400 MHz, DMSO-d6) δ = 8.90 (s, 1H), 8.47 (s, 1H), 7.78 (s, 1H), 7.72 (d, 1H), 6.91 (t, 1H), 6.17 (s, 1H), 2.65 (s, 6H).

HPLC-MS: Rt 2.735; m/z 229.9 (MH+).

Method F: Example F1: 4-(benzo[b]thiophen-3-yl)-2-methyl-6-phenyl-1,4-dihydropyridine-3,5-dicarbonitrile

A mixture of thianaphthene-3-carboxaldehyde (0.104 g, 0.64 mmol), 3-aminocrotonitrile (0.052 g, 0.64 mmol), benzoylacetonitrile (0.0897 g, 0.62 mmol) and acetic acid (0.035 mL, 0.64 mmol) in isopropyl alcohol (3.5 mL) was heated to 100°C and left to stir for 16 hours. The mixture was allowed to cool to RT and concentrated. The residue was basified with aq. sodium bicarbonate, and the resulting solid was filtered off and purified by column chromatography (3:1 Hexane: Ethyl acetate) rendering a light-yellow solid (0.0162 g, 7 %).
1H-NMR (400 MHz, DMSO-d6) δ = 2.12 (s, 3H), 5.17 (s, 1H), 7.39-7.49 (m, 2H), 7.50-7.59 (m 5H), 7.78 (s, 1H), 8.01 (dd, 1H), 8.06 (dd, 1H), 9.92 (s, 1H).
HPLC-MS: Rt 4.329 min, m/z 354.1 (MH+).

The following examples were synthesized according to Method F.

Example F2: 4-(benzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3,5-dicarbonitrile

1H-NMR (400 MHz, DMSO-d6) δ = 0.95 (s, 4H), 2.06 (s, 3H), 4.98 (s, 1H), 7.41 (s, 2H), 7.64 (s 1H), 7.84 (s, 1H), 8.03 (s, 1H), 8.82 (s, 1H).

HPLC-MS: Rt 4.138 min, m/z 318.0 (MH+).

Example F3: 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carbonitrile

1H-NMR (400 MHz, DMSO-d6) δ = 2.00 (s, 6H), 2.31 (s, 3H), 5.16 (s, 1H), 7.30-7.48 (m, 3H), 7.97 (d 1H), 8.04 (d, 1H), 9.30 (s, 1H).

HPLC-MS: Rt 3.816 min, m/z 175.1 (MH+-134).

Example F4: Ethyl 4-(benzo[b]thiophen-3-yl)-5-cyano-2,6-dimethyl-1,4-dihydropyridine -3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.90 (t, 3H), 2.02 (s, 3H), 2.30 (s, 3H), 3.85 (dd, 2H), 5.02 (s, 1H), 7.31-7.45 (m 3H), 7.91 (d, 1H), 7.96 (d, 1H), 9.30 (s, 1H).

HPLC-MS: Rt 4.418 min, m/z 205.1 (MH+-134).

Example F5: Ethyl 4-(benzo[b]thiophen-3-yl)-5-cyano-2-methyl-6-phenyl-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.98 (t, 3H), 2.37 (s, 3H), 3.92 (q, 2H), 5.17 (s, 1H), 7.41 (ddd 2H), 7.49 (d, 5H), 8.00 (t, 2H), 9.60 (s, 1H).

HPLC-MS: Rt 4.946 min, m/z 267.1 (MH+-134).

Example F6: Ethyl 4-(benzo[b]thiophen-3-yl)-5-cyano-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.80-0.98 (m, 6H), 0.96 -1.06 (m, 1H), 2.03 (s, 3H), 2.73-2.84 (m, 1H), 3.82-3.95 (m, 2H), 5.02 (s, 1H), 7.32 (s, 1H), 7.33-7.44 (m, 2H), 7.90 (d, 1H), 7.96 (d, 1H), 8.23 (s, 1H).

HPLC-MS: Rt 4.693 min, m/z 231.1 (MH+-134).

Example F7: Methyl 4-(benzo[b]thiophen-3-yl)-5-cyano-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.79-0.95 (m, 3H), 1.02 (ddd, 1H), 2.03 (s, 3H), 2.78 (dq, 1H), 3.45 (s, 3H), 5.01 (s,1H), 7.30 (s, 1H), 7.39 (dt, 2H), 7.90 (d, 1H),7.96 (dd, 1H), 8.28 (s, 1H).

HPLC-MS: Rt 4.438 min, m/z 217.1 (MH+-134).

Method G: Example G1: 1,1'-(4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-diyl)diethanone

A mixture of thianaphthene-3-carboxaldehyde (0.2 g, 1.23 mmol), acetylacetone (0.25 mL, 2.47 mmol) and aqueous ammonium hydroxide solution (38-40%, 0.12 mL) in ethanol (1 mL) was heated to 90°C and left to stir for 16 hours. The mixture was allowed to cool to RT and was then poured into 10 ml of cold water. The precipitate formed was filtered off, dried and washed with cold diethyl ether. The desired product was obtained as a strongly yellow solid (0.193 g, 48 %).
1H-NMR (400 MHz, DMSO-d6) δ = 2.17 (s, 6H), 2.29 (s, 6H), 5.52 (s, 1H), 7.23 (s, 1H), 7.33 (dt 2H), 7.88 (d, 1H), 8.12 (d, 1H), 9.08 (s, 1H).
HPLC-MS: Rt 3.668 min, m/z 192.1 (MH+-134).

The following examples were synthesized according to Method G.

Example G2: 1,1'-(4-(6-hydroxybenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-diyl)bis(ethan-1-one)

1H-NMR (400 MHz, DMSO-d6) δ = 2.28 (s, 12H), 5.29 (s, 1H), 6.76 (d, 2H), 7.10 (s 1H), 7.45 (d, 1H), 9.10 (s, 1H), 9.40 (s, 1H).

HPLC-MS: Rt 2.950 min, m/z 192.1(MH+-150).

Example G3: 1,1'-(4-(6-Methoxybenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-diyl)diethanone

1H-NMR (400 MHz, DMSO-d6) δ = 2.29 (d, 12H), 3.75 (s, 3H), 5.32 (s, 1H), 6.86 (d 1H), 6.89 (dd, 1H), 7.36 (d, 1H), 7.55 (d, 1H), 9.12 (s, 1H).

HPLC-MS: Rt 3.676 min, m/z 192 (MH+-164).

Example G4: 1,1'-(4-(Benzo[b]thiophen-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridine-3,5-diyl)diethanone

1H-NMR (400 MHz, DMSO-d6) δ = 0.69 (dd, 2H), 0.74 (dd, 4H), 0.78-0.84 (m, 2H), 2.18-2.25 (m, 2H), 2.27 (s, 6H), 5.69 (s, 1H), 7.21 (s 1H), 7.26-7.36 (m, 2H), 7.89 (d, 1H), 7.97 (d, 1H), 83.99 (s, 1H).

Method H: Example H1: 1,1'-(4-(5-chlorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-diyl)diethanone

A mixture of 5-chlorobenzo[b]thiophene-3-carbaldehyde (0.05 g, 0.26 mmol), 4-amino-3-penten-2-one (0.60 g, 0.52 mmol) and acetic acid (0.015 mL) in isopropanol (1.5 mL) was heated to 100°C and left to stir for 16 hours. The mixture was allowed to cool to RT and was then treated with saturated aqueous sodium bicarbonate solution (10 mL). The solid was filtered off, washed with water, dried and purified by column chromatography (3:1 hexane: ethyl acetate) affording a fine yellow solid (8.8 mg, 9.5%).
1H-NMR (400 MHz, DMSO-d6) δ = 2.17 (s, 6H), 2.31 (s, 6H), 5.44 (s, 1H), 7.33 (d, 2H), 7.92 (d, 1H), 8.23 (s, 1H), 9.15 (s, 1H).
HPLC-MS: Rt 4.072 min, m/z 192.1 (MH+-168).

The following examples were synthesized according to Method H.

Example H2: 1,1'-(4-(5-bromobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-diyl)diethanone

1H-NMR (400 MHz, DMSO-d6) δ = 2.17 (s, 6H), 2.31 (s, 6H), 5.44 (s, 1H), 7.30 (d, 1H), 7.44 (d, 1H), 7.86 (d, 1H), 8.38 (s, 1H), 9.16 (s, 1H).

Example H3: 1,1'-(4-(5-fluorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-diyl)bis(ethan-1-one)

1H-NMR (400 MHz, DMSO-d6) δ = 9.15 (s, 1H), 7.92 (dd, J = 8.1, 4.2 Hz, 2H), 7.34 (s, 1H), 7.20 (td, J = 8.8, 2.3 Hz, 1H), 5.44 (s, 1H), 2.32 (s, 6H), 2.18 (s, 6H).

Example H4: 1,1'-(2,6-dimethyl-4-(5-morpholinobenzo[b]thiophen-3-yl)-1,4-dihydropyridine-3,5-diyl)bis(ethan-1-one)

1H-NMR (400 MHz, DMSO-d6) δ = 9.11 (s, 1H), 7.70 (d, 1H), 7.49 (d, 1H), 7.20 (s, 1H), 7.09 (dd, 1H), 5.48 (s, 1H), 3.80 (t, 4H), 3.12 (t, 4H), 2.27 (s, 6H), 2.19 (s, 6H).

Example H5: 1,1'-(2,6-dimethyl-4-(5-(4-methylpiperazin-1-yl)benzo[b]thiophen-3-yl)-1,4-dihydropyridine-3,5-diyl)bis(ethan-1-one)

1H-NMR (400 MHz, DMSO-d6) δ = 9.12 (s, 1H), 7.67 (d, 1H), 7.48 (d, 1H), 7.19 (s, 1H), 5.48 (s, 1H), 3.80 (t, 4H), 3.12 (t, 4H), 2.27 (s, 6H), 2.19 (s, 6H).

Example H6: 1,1'-(4-(5-(benzylamino)benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-diyl)bis(ethan-1-one)

1H-NMR (400 MHz, DMSO-d6) δ = 8.98 (s, 1H), 7.50 (d, 1H), 7.43 (d, 2H), 7.32 (t, 2H), 7.23 (t, 1H), 7.14 (d, 1H), 7.08 (s, 1H), 6,77 (dd, 1H), 6.21 (t, 1H), 5.33 (s, 1H), 4.30 (d, 2H), 2.22 (s, 6H), 2.09 (s, 6H).

Example H7: 3-(3,5-diacetyl-2,6-dimethyl-1,4-dihydropyridin-4-yl)benzo[b]thiophene-5-carbonitrile

1H-NMR (400 MHz, DMSO-d6) δ = 2.17 (s, 6H), 2.33 (s, 6H), 5.49 (s, 1H), 7.43 (s, 1H), 7.67 (dd, 1H), 8.13 (d, 1H), 8.66 (s, 1H), 9.20 (s, 1H).

HPLC-MS: Rt 3.563; m/z 351.1 (MH+).

Example H8: 3-(3,5-diacetyl-2,6-dimethyl-1,4-dihydropyridin-4-yl)benzo[b]thiophene-5-carboxylic acid

1H-NMR (400 MHz, DMSO-d6) δ = 9.13 (s, 1H), 8.82 (s, 1H), 7.96 (d, 1H), 7.85 (d, 1H), 7.33 (s, 1H), 5.54 (s, 1H), 2.30 (s, 6H), 2.18 (s, 6H).

Example H9: N-cyclopropyl-3-(3,5-diacetyl-2,6-dimethyl-1,4-dihydropyridin-4-yl)benzo[b]thiophene-5-carboxamide

1H-NMR (400 MHz, DMSO-d6) δ = 9.13 (s, 1H), 8.64 (s, 1H), 8.42 (d, 1H), 7.93 (d, 1H), 7.72 (d, 1H), 7.32 (s, 1H), 5.53 (s, 1H), 2.89 (m, 1H), 2.29 (s, 6H), 2.18 (s, 6H), 0.73 (m, 2H), 0.61 (m, 2H).

Example H10: N-(cyclopropylmethyl)-3-(3,5-diacetyl-2,6-dimethyl-1,4-dihydropyridin-4-yl)benzo[b]thiophene-5-carboxamide

1H-NMR (400 MHz, DMSO-d6) δ = 9.12 (s, 1H), 8.68 (s, 1H), 8.50 (d, 1H), 7.95 (d, 1H), 7.78 (d, 1H), 7.32 (s, 1H), 5.53 (s, 1H), 3.26 (t, 2H), 2.29 (s, 6H), 2.18 (s, 6H), 1.08 (m, 1H), 0.45 (m, 2H), 0.28 (m, 2H).

Example H11: 1,1'-(2,6-Dimethyl-4-(5-(4-methylpiperazine-1-carbonyl)benzo[b]thiophen-3-yl)-1,4-dihydropyridine-3,5-diyl)bis(ethan-1-one)

1H-NMR (400 MHz, CDCl3) δ = 8.21 (s, 1H), 7.82 (d, 1H), 7.42 (d, 1H), 7.21 (d, 1H), 6.17 (s, 1H), 5.61 (s, 1H), 3.77 (d, 4H), 2.54 (m, 4H), 2.38 (s, 3H), 2.34 (s, 3H), 2.22 (s, 3H), 2.08 (s, 3H).

Example H12: 1,1'-(2,6-Dimethyl-4-(5-(morpholine-4-carbonyl)benzo[b]thiophen-3-yl)-1,4-dihydropyridine-3,5-diyl)bis(ethan-1-one)

1H-NMR (400 MHz, DMSO-d6) δ = 9.15 (s, 1H), 8.21 (s, 1H), 7.97 (d, 1H), 7.35 (dd, 1H), 7.32 (s, 1H), 5.50 (s, 1H), 3.67 (m, 8H), 2.31 (s, 6H), 2.16 (s, 6H).

Example H13: 1,1'-(2,6-Dimethyl-4-(thieno[3,2-c]pyridin-3-yl)-1,4-dihydropyridine-3,5-diyl)bis(ethan-1-one)

1H-NMR (400 MHz, DMSO-d6) δ = 9.35 (s, 1H), 9.18 (s, 1H), 8.36 (d, 1H), 7.95 (d,1H), 7.34 (s, 1H), 5.58 (s, 1H), 2.31 (s, 6H), 2.19 (s, 6H).

Example H14: 1,1'-(2,6-Dimethyl-4-(thieno[2,3-c]pyridin-3-yl)-1,4-dihydropyridine-3,5-diyl)bis(ethan-1-one)

1H-NMR (400 MHz, DMSO-d6) δ = 9.17 (s, 1H), 9.13 (d, 1H), 8.44 (d, 1H), 8.05 (dd, 1H), 7.57 (s, 1H), 5.51 (s, 1H), 2.31 (s, 6H), 2.17 (s, 6H).

Example H15: 1,1'-(2,6-Dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3,5-diyl)bis(ethan-1-one)

1H-NMR (400 MHz, DMSO-d6) δ = 9.15 (s, 1H), 8.49 (d, 2H), 7.43 (s, 1H), 7.33 (s, 1H), 5.47 (s, 1H), 2.32 (s, 6H), 2.18 (s, 6H).

Method I: Example I1: Dimethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-2,3-dicarboxylate

A mixture of benzo[b]thiophene-3-carbaldehyde (0.20 g, 1.23 mmol), pentane-2,4-dione (0.123 g, 1.23 mmol), dimethyl but-2-ynedioate (0.182 mL, 1.48 mmol), ammonium acetate (0.143 g, 1.85 mmol), iodine (0.094 g, 0.37 mmol) and two drops of acetic acid were stirred in methanol (3 mL) at 60°C for 12h. The solvent was removed under reduced pressure. The residue was dissolved in ethyl acetate and the organic layer was washed twice with saturated NaS2O3 solution and brine, dried (Na2SO4), filtered and concentrated. The residue was purified by column chromatography (2:1 hexane: ethyl acetate) affording a yellow solid (0.028 g, 6%).
1H-NMR (300 MHz, CDCl3) δ = 7.94 - 7.80 (m, 2H), 7.46 - 7.29 (m, 2H), 7.19 (s, 1H), 6.38 (bs, 1H), 5.38 (s, 1H), 3.81 (s, 3H), 3.52 (s, 3H), 2.43 (s, 3H), 2.04 (s, 3H).
HRMS (APCI) m/z calculated C20H20NO5S [M+1]: 386.1056, found: 386.1058.

Example I2: Dimethyl 5-acetyl-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-2,3-dicarboxylate

The title compound was prepared according to Method I affording a yellow solid (0.04 g, 2%).
1H-NMR (300 MHz, CDCl3) δ = 8.24 (d, J = 7.9 Hz, 1H), 7.70 (d, J = 7.2 Hz, 1H), 7.50 (t, J = 7.9 Hz, 1H), 7.34 (s, 1H), 6.43 (bs, 1H), 5.41 (s, 1H), 3.84 (s, 3H), 3.55 (s, 3H), 2.44 (s, 3H), 2.11 (s, 3H).
HRMS (APCI) m/z calculated C21H19N2O5S [M+1]: 411.1009, found: 411.1006.

Method J: Example J1: Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate

A mixture of thianaphthene-3-carboxaldehyde (0.106 g, 0.65 mmol), methyl 3-cyclopropyl-3-oxopropanoate (0.071 g, 0.72 mmol) and 4-amino-3-penten-2-one (0.088 g, 0.61 mmol) and acetic acid (0.035 mL) in isopropylalcohol (1.5 mL) was heated to 100°C and left to stir for 19 hours. The mixture was allowed to cool to RT and was then treated with saturated aqueous sodium bicarbonate solution (10 mL). The solid was filtered off, washed with water, dried and purified by column chromatography (4:1 hexane: ethyl acetate) affording a fine yellow solid (0.075 g, 44 %).
1H-NMR (400 MHz, DMSO-d6) δ = 0.76 (s, 2H), 0.96 (d, 2H), 2.12 (s, 3H), 2.33 (s, 3H), 2.69 (s, 1H), 3.58 (s, 3H), 5.43 (s, 1H), 7.15 (s, 1H), 7.31 (s, 1H), 7.38 (s, 1H), 7.90 (s, 2H), 8.05 (d, 1H).
HPLC-MS: Rt 4.302 min, m/z 234.1 (MH+-133).

A second reaction product was isolated by column chromatography:

Example J1-a: Dimethyl 4-(benzo[b]thiophen-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.56 (s, 2H), 0.82 (s, 2H), 0.90 (d, 2H), 1.04 (s, 2H), 2.56 (m, 2H), 3.55 (s, 5H), 5.37 (s, 1H), 7.05 (s, 1H), 7.11 (s 1H), 7.32 (t, 1H), 7.39 (t, 1H), 7.90 (d, 1H), 7.98 (s, 1H).

HPLC-MS: Rt 4.935; m/z 276.1 (MH+).

The following examples were synthesized according to Method J.

Example J2: Ethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 1.1 (t, 3H), 2.11 (s, 3H), 2.25 (s, 3H), 2.31 (s, 3H), 3.99 (d, 2H), 5.40 (s, 1H), 7.23 (s, 1H), 7.31 (t 1H), 7.37 (m, 1H), 7.89 (d, 1H), 8.08 (d, 1H), 9.02 (s, 1H).

HPLC-MS: Rt 4.284 min, m/z 222.1 (MH+-134).

Example J3: Methyl 5-acetyl-4-(5-chlorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 2.13 (s, 3H), 2.25 (s, 3H), 2.33 (s, 3H), 3.54 (s, 3H), 5.33 (s, 1H), 7.33 (s, 1H), 7.93 (d, 1H), 8.11 (d, 1H), 8.52 (s, 1H), 9.11 (s, 1H). HPLC-MS: Rt 4.431 min, m/z 208.1 (MH+-168).

Example J6: 1,1'-(4-(benzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3,5-diyl)diethanone

1H-NMR (400 MHz, DMSO-d6) δ = 0.72 (m, 2H), 0.80 (m, 2H), 2.16 (s, 3H), 2.26 (d, 1H), 2.33 (s, 6H), 5.60 (s, 1H), 7.21 (s, 1H), 7.32 (dd, 2H), 7.89 (d, 1H), 8.04 (d, 1H), 9.02 (s, 1H).

HPLC-MS: Rt 4.692; m/z 352.0 (MH+).

Example J10: 1-(4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-5-(methylsulfonyl)-1,4-dihydro-pyridin-3-yl)ethanone

1H-NMR (400 MHz, DMSO-d6) δ = 2.16 (s, 3H), 2.29 (d, 6H), 2.47 (s, 3H), 5.47 (s, 1H), 7.33 (t, 1H), 7.43 - 7.37 (m, 2H), 7.93 (d, 1H), 8.07 (d, 1H), 9.26 (s, 1H).

HPLC-MS: Rt 3.395 min, m/z 228.0 (MH+-134).

Example J11: Methyl 5-acetyl-4-(5-fluorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 9.10 (s, 1H), 7.93 (dd, J = 8.8, 5.2 Hz, 1H), 7.81 (dd, J = 11.0, 2.5 Hz, 1H), 7.35 (s, 1H), 7.20 (td, J = 8.8, 2.6 Hz, 1H), 5.33 (s, 1H), 3.54 (s, 3H), 2.32 (s, 3H), 2.26 (s, 3H), 2.12 (s, 3H).

HPLC-MS: Rt 4.345; m/z 360.0 (MH+).

Example J12: Methyl 5-acetyl-4-(5-bromobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 9.11 (s, 1H), 8.26 (d, 1H), 7.88 (d, 1H), 7.45 (d, 1H), 7.31 (s, 1H), 5.34 (s, 1H), 3.58 (s, 3H), 2.34 (s, 3H), 2.25 (s, 3H), 2.16 (s, 3H).

Example J13: Methyl 5-acetyl-4-(5-cyanobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 9.16 (s, 1H), 8.53 (s, 1H), 8.15 (d, 1H), 7.67 (d, 1H), 7.46 (s, 1H), 5.40 (s, 1H), 3.55 (s, 3H), 2.35 (s, 3H), 2.27 (s, 3H), 2.17 (s, 3H).

Example J14: 3-(3-acetyl-5-(methoxycarbonyl)-2,6-dimethyl-1,4-dihydropyridin-4-yl)benzo[b]thiophene-5-carboxylic acid

1H-NMR (400 MHz, DMSO-d6) δ = 12.94 (s, 1H), 9.12 (s, 1H), 8.77 (s, 1H), 8.00 (d, 1H), 7.85 (d, 1H), 7.32 (s, 1H), 5.52 (s, 1H), 3.56 (s, 3H), 2.35 (s, 3H), 2.24 (s, 3H), 2.12 (s, 3H).

Example J15: Methyl 5-acetyl-4-(5-(cyclopropylcarbamoyl)benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 9.06 (s, 1H), 8.59 (s, 1H), 8.45 (d, 1H), 7.91 (d, 1H), 7.72 (dd, 1H), 7.26 (s, 1H), 5.39 (s, 1H), 3.53 (s, 3H), 2.85 (m, 1H), 2.37 (s, 3H), 2.19 (s, 3H), 2.06 (s, 3H), 0.69 (m, 2H), 0.58 (m, 2H).

Example J16: Methyl 5-acetyl-4-(5-((cyclopropylmethyl)carbamoyl)benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 9.10 (s, 1H), 8.66 (s, 1H), 8.60 (d, 1H), 7.97 (d, 1H), 7.81 (d, 1H), 7.30 (s, 1H), 5.43 (s, 1H), 3.57 (s, 3H), 3.20 (m, 2H), 2.85 (m, 1H), 2.34 (s, 3H), 2.22 (s, 3H), 2.10 (s, 3H), 0.45 (m, 2H), 0.26 (m, 2H).

Example J17: Methyl 5-acetyl-2,6-dimethyl-4-(5-(4-methylpiperazine-1-carbonyl)benzo[b]thiophen-3-yl)-1,4-dihydropyridine-3-carboxylate 1H-NMR (400 MHz, CDCl3) δ = 8.22 (s, 1H), 7.82 (d, 1H), 7.36 (dd, 1H), 7.22 (d, 1H), 5.94 (s, 1H), 5.46 (s, 1H), 3.77 (d, 4H), 3.65 (s, 3H), 2.53 (m, 4H), 2.38 (s, 3H), 2.36 (s, 3H), 2.32 (s, 3H), 2.12 (s, 3H). Example J18: Methyl 5-acetyl-2,6-dimethyl-4-(5-(morpholine-4-carbonyl)benzo[b]thiophen-3-yl)-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 9.11 (s, 1H), 8.13 (s, 1H), 7.97 (d, 1H), 7.35 (d, 1H), 7.32 (s, 1H), 5.39 (s, 1H), 3.64 (m, 8H), 3.36 (s, 3H), 2.32 (s, 3H), 2.25 (s, 3H), 2.12 (s, 3H).

Example J19: Methyl 5-acetyl-2,6-dimethyl-4-(thieno[3,2-c]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 9.27 (d, 1H), 9.13 (s, 1H), 8.36 (d, 1H), 7.97 (dd,1H), 7.35 (s, 1H), 5.48 (s, 1H), 3.52 (s, 3H), 2.32 (s, 3H), 2.27 (s, 3H), 2.14 (s, 3H).

Example J20: Methyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-c]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 9.15 (s, 1H), 9.12 (s, 1H), 8.46 (d, 1H), 7.97 (dd,1H), 7.59 (s, 1H), 5.41 (s, 1H), 3.52 (s, 3H), 2.32 (s, 3H), 2.27 (s, 3H), 2.12 (s, 3H).

Example J21: Methyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 9.10 (s, 1H), 8.50 (dd, 1H), 8.40 (dd, 1H), 7.45 (dd,1H), 7.35 (s, 1H), 5.36 (s, 1H), 3.52 (s, 3H), 2.32 (s, 3H), 2.27 (s, 3H), 2.13 (s, 3H).

Example J22: Methyl 5-acetyl-2-cyclopropyl-4-(5-fluorobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.76 (m, 1H), 0.87 (m, 2H), 1.05 (m, 1H), 2.14 (s, 3H), 2.33 (s, 3H), 2.70 (m, 1H), 3.57 (s, 3H), 5.34 (s, 1H), 7.21 (t, 1H), 7.27 (s, 1H), 7.80 (s, 1H), 7.93 (dd, 1H), 7.96 (s, 1H).

HPLC-MS: Rt 4.645 m/z 234.1 (MH+).

A second reaction product was isolated by column chromatography:

Example J22-a: Dimethyl 2,6-dicyclopropyl-4-(5-fluorobenzo[b]thiophen-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.60 (m, 2H), 0.68 (m, 2H), 1.00 (m, 4H), 2.76 (m, 2H), 3.61 (s, 6H), 5.42 (s, 1H), 5.54 (s, 1H), 7.05 (t, 1H), 7.14 (s, 1H), 7.71 (m, 1H).

HPLC-MS: Rt 5.256; m/z 428.1 (MH+).

Example J23: Methyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 8.51 (dd, 1H), 8.39 (dd, 1H), 7.95 (s, 1H), 7.44 (dd, 1H), 7.27 (s, 1H), 5.38 (s, 1H), 3.56 (s, 3H), 2.71 (dd, 1H), 2.34 (s, 3H), 2.14 (s, 3H), 0.84 (t, 4H).

A second reaction product was isolated by column chromatography:

Example J23-a: Dimethyl 2,6-dicyclopropyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.57 (q, 2H), 0.83 (m, 2H), 0.91 (t, 2H), 1.04 (q, 2H), 2.57 (td, 2H), 3.54 (s, 6H), 5.31 (s, 1H), 7.09 (s, 1H), 7.26 (s, 1H), 7.45 (dd, 1H), 8.30 (dd, 1H),8.52 (m, 1H).

HPLC-MS: Rt 4.848; m/z 411.0 (MH+).

Example J24: Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.81 (m, 7H), 1.07 (dt, 1H), 2.14 (m, 1H), 2.28 (s, 3H), 2.74 (m, 1H), 3.57 (s, 3H), 5.49 (s, 1H), 7.14 (s, 1H), 7.31 (t, 1H), 7.36 (t, 1H), 7.90 (m, 2H), 7.98 (d, 1H).

HPLC-MS: Rt 4.670; m/z 260.1 (MH+).

Example J25: Methyl 5-acetyl-4-(5-chlorobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.84 (m, 3H), 1.05 (m, 1H), 2.15 (s, 3H), 2.34 (s, 3H), 2.70 (ddd, 1H), 3.58 (s, 3H), 5.35 (s, 1H), 7.26 (s, 1H), 7.35 (dd, 1H), 7.94 (d, 1H), 7.97 (s, 1H), 8.08 (d, 1H).

HPLC-MS: Rt 5.312; m/z 402.9 (MH+).

A second reaction product was isolated by column chromatography:

Example J25-a: Dimethyl 4-(5-chlorobenzo[b]thiophen-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 2.28 (s, 6H), 3.50 (s, 6H), 5.26 (s, 1H), 7.33 (s, 2H), 7.93 (d, 1H), 8.00 (s, 1H), 9.10 (s, 1H).

HPLC-MS: Rt 4.773; m/z 224.1 (MH+).

Example J26: Methyl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.82 (m, 3H), 1.05 (dq, 1H), 2.13 (s, 3H), 2.33 (s, 3H), 2.69 (m, 1H), 3.56 (s, 3H), 5.38 (s, 1H), 7.28 (s, 1H), 7.37 (t, 1H), 7.57 (d, 1H), 7.93 (s, 1H), 8.10 (d, 1H).

HPLC-MS: Rt 5.355; m/z 448.8 (MH+).

Example J27: Methyl 5-acetyl-2-cyclopropyl-4-(7-(cyclopropylcarbamoyl)benzo[b] thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.59 (m, 2H), 0.71 (m, 2H), 0.84 (dd, 4H), 2.10 (s, 3H), 2.33 (s, 3H), 2.68 (m, 1H), 2.88 (m, 1H), 3.55 (s, 3H), 5.40 (s, 1H), 7.21 (s, 1H), 7.47 (m, 1H), 7.86 (d, 1H), 7.89 (s, 1H), 8.23 (d, 1H), 8.63 (d, 1H).

HPLC-MS: Rt 4.354; m/z 451.0 (MH+).

Example J28: Methyl 5-acetyl-4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.85 (m, 3H), 1.06 (m, 1H), 2.14 (s, 2H), 2.34 (s, 3H), 2.70 (t, 1H), 3.54 (s, 3H), 5.42 (d, 1H), 7.60 (dd, 1H), 7.38 (s, 1H), 7.91 (d, 1H), 7.98 (s, 1H), 8.42 (dd, 1H).

HPLC-MS: Rt 4.848; m/z 393.0 (MH+).

Example J28-a: Dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.58 (td, 2H), 0.88 (m, 4H), 1.03 (q, 2H), 2.56 (m, 2H), 3.53 (s, 6H), 5.36 (s, 1H), 7.10 (s, 1H), 7.38 (s, 1H), 7.62 (m, 1H), 7.92 (d, 1H), 8.32 (d, 1H).

HPLC-MS: Rt 5.493; m/z 435.0 (MH+).

Example J29: Methyl 5-acetyl-2-cyclopropyl-4-(7-(methoxycarbonyl) benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.76 (m, 1H), 0.87 (m, 2H), 1.06 (dt, 1H), 2.12 (s, 3H), 2.34 (s, 3H), 2.69 (m, 1H), 3.55 (s, 3H), 3.93 (s, 3H), 5.43 (s, 1H), 7.28 (s, 1H), 7.57 (t, 1H), 7.92 (s, 1H), 8.04 (d, 1H), 8.39 (d, 1H).

HPLC-MS: Rt 4.875; m/z 426.1 (MH+).

A second reaction product was isolated by column chromatography:

Example J29-a: Dimethyl 2,6-dicyclopropyl-4-(7-(methoxycarbonyl)benzo[b]thiophen-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.56 (td, 2H), 0.87 (m, 4H), 1.05 (td, 2H), 2.57 (m, 2H), 3.53 (s, 6H), 3.93 (s, 3H), 5.38 (s, 1H), 7.06 (s, 1H), 7.27 (s, 1H), 7.58 (t, 1H), 8.05 (d, 1H), 8.30 (d, 1H).

HPLC-MS: Rt 5.520; m/z 468.1 (MH+).

The ester functionality on the benzothiophene ring of methyl 5-acetyl-2-cyclopropyl-4-(7-(methoxycarbonyl) benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate (Example J29) was hydrolyzed as follows:

Example J30: 3-(3-Acetyl-6-cyclopropyl-5-(methoxycarbonyl)-2-methyl-1,4-dihydropyridin-4-yl)benzo[b]thiophene-7-carboxylic acid

A mixture of methyl 5-acetyl-2-cyclopropyl-4-(7-(methoxycarbonyl)benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate (0.004 g, 0.094 mmol) and 1M NaOH (0.47 mL, 0.470 mmol) in 1 mL THF was stirred at 40 °C over the weekend. The reaction mixture was diluted with 0.1 M NaOH and washed with DCM. The aqueous layer was cooled to 0°C and 4M HCl were added until a solid started to precipitate. Then 1M HCl was dropwise added until the pH reached 2-3. The precipitate was filtered off, washed with water and purified by column chromatography (DCM/MeoH) to give the title compound (0.0116 g, 30%).
1H-NMR (400 MHz, DMSO-d6) δ = 0.83 (m, 2H), 1.06 (m, 2H), 2.12 (s, 3H), 2.34 (s, 3H), 2.69 (m, 1H), 3.56 (s, 3H), 5.43 (s, 1H), 7.24 (s, 1H), 7.54 (t, 1H), 7.91 (s, 1H), 8.01 (d, 1H), 8.34 (d, 1H), 13.46 (s, 1H).
HPLC-MS: Rt 3.039; m/z 410.0 (MH+).

Example J31: Benzyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

The title compound was obtained following Method J.
1H-NMR (400 MHz, DMSO-d6) δ = 0.81 (m, 3H), 1.08 (m, 1H), 2.15 (s, 3H), 2.34 (s, 3H), 2.72 (dt, 1H), 5.06 (d, 2H), 5.41 (s, 1H), 7.17 (dd, 2H), 7.24 (s, 1H), 7.27 (m, 2H), 7.30 (dd, 2H), 8.00 (s, 1H), 8.31 (d, 1H), 8.47 (d, 1H).
HPLC-MS: Rt 4.976; m/z 445.1 (MH+).

Example J32: Pyridin-4-ylmethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.83 (m, 3H), 1.09 (m, 1H), 2.16 (s, 3H), 2.33 (s, 3H), 2.73 (m, 1H), 5.11 (q, 2H), 5.51 (s, 1H), 7.08 (d, 2H), 7.18 (s, 1H), 7.28 (m, 2H), 7.90 (d, 1H), 8.02 (d, 2H), 8.41 (d, 2H).

HPLC-MS: Rt 4.673; m/z 445.1 (MH+).

A second reaction product was isolated by column chromatography:

Example J32-a: bis(pyridin-4-ylmethyl) 2,6-dicyclopropyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.62 (m, 2H), 0.86 (dd, 4H) 1.06 (dt, 2H), 2.57 (m, 2H), 5.08 (m, 4H), 5.42 (s, 1H), 7.06 (d, 4H), 7.19 (dd, 1H), 7.28 (s, 1H), 7.33 (s, 1H), 8.15 (dd, 1H), 8.40 (d, 4H), 8.46 (dd, 1H).

HPLC-MS: Rt 4.445; m/z 565.2 (MH+).

Example J33: Pyridin-4-ylmethyl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 2.15 (s, 3H), 2.31 (d, 6H), 5.06 (dd, 2H), 5.43 (s, 1H), 7.00 (d, 2H), 7.24 (t, 1H), 7.39 (s, 1H), 7.53 (d, 1H), 8.08 (d, 1H), 8.38 (d, 2H), 9.18 (s, 1H).

HPLC-MS: Rt 4.799; m/z 497.0 (MH+).

Example J34: Pyridin-4-ylmethyl 5-acetyl-4-(5-chlorobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.86 (m, 3H), 1.09 (dd, 1H), 2.18 (s, 3H), 2.34 (s, 3H), 2.75 (ddd, 1H), 5.11 (d, 2H), 5.44 (s, 1H), 7.05 (d, 2H),7.30 (s, 1H) 7.32 (dd, 1H),7.94 (d, 1H), 8.07 (s, 1H), 8.10 (d, 1H), 8.40 (d, 2H).

HPLC-MS: Rt 5.002; m/z 479.0 (MH+).

Example J35: Pyridin-4-ylmethyl 5-acetyl-4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.86 (m, 3H), 1.10 (m, 1H), 2.18 (s, 3H), 2.35 (s, 3H), 2.75 (t, 1H), 5.08 (dd, 2H), 5.50 (s, 1H), 7.03 (d, 2H), 7.42 (s, 1H), 7.46 (t, 1H), 7.87 (d, 1H), 8.08 (s, 1H), 8.39 (dd, 3H).

HPLC-MS: Rt 4.603; m/z 470.1 (MH+).

Example J36: Pyridin-4-ylmethyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.85 (dt, 3H), 1.10 (m, 1H), 2.18 (s, 2H), 2.34 (s, 2H), 2.75 (dd, 1H), 5.10 (dd, 2H), 5.45 (s, 1H), 7.06 (d, 2H), 7.32 (m, 2H), 8.06 (s, 1H), 8.36 (d, 1H), 8.40 (d, 2H), 8.48 (d, 1H).

HPLC-MS: Rt 3.943; m/z 446.1 (MH+).

Example J37: Benzyl 5-acetyl-2-cyclopropyl-4-(7-(methoxycarbonyl)benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.80 (dd, 3H), 1.08 (d, 1H), 2.13 (s, 3H), 2.34 (s, 3H), 2.71 (t, 1H), 3.93 (s, 3H), 5.05 (s, 2H), 5.47 (s, 1H), 7.18 (d, 2H), 7.26 (s, 4H), 7.42 (t, 1H), 8.00 (m, 2H), 8.32 (d, 1H).

HPLC-MS: Rt 5.546; m/z 502.2 (MH+).

A second reaction product was isolated by column chromatography:

Example J37-a: Dibenzyl 2,6-dicyclopropyl-4-(7-(methoxycarbonyl)benzo[b]thiophen-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.55 (dd, 2H), 0.82 (dd, 4H), 1.05 (m, 2H), 2.57 (m, 2H), 3.93 (s, 3H), 5.01 (q, 4H), 5.44 (s, 1H), 7.15 (d, 4H), 7.26 (m, 8H), 7.99 (d, 1H), 8.12 (d, 1H).

HPLC-MS: Rt 6.574; m/z 621.3 (MH+).

Example J38: Benzyl 5-acetyl-4-(7-(methoxycarbonyl)benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 2.11 (s, 3H), 2.28 (s, 3H), 2.32 (s, 3H), 3.92 (s, 3H), 5.00 (s, 2H), 5.44 (s, 1H), 7.14 (d, 2H), 7.24 (d, 3H), 7.33 (s, 1H), 7.41 (dd, 1H), 8.00 (d, 1H), 8.33 (d, 1H), 9.12 (s, 1H).

HPLC-MS: Rt 5.546; m/z 502.2 (MH+).

Example J39: Methyl 3-(3,5-diacetyl-2,6-dimethyl-1,4-dihydropyridin-4-yl)benzo[b]thiophene-7-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 2.16 (s, 6H), 2.31 (s, 6H), 3.92 (s, 3H), 5.52 (s, 1H), 7.34 (s, 1H), 7.55 (t, 1H), 8.03 (d, 1H), 8.50 (d, 1H), 9.13 (s, 1H).

HPLC-MS: Rt 4.224; m/z 384.1 (MH+).

Example J40: 3-(3,5-diacetyl-2,6-dimethyl-1,4-dihydropyridin-4-yl)benzo[b]thiophene-7-carbonitrile

1H-NMR (400 MHz, DMSO-d6) δ = 2.17 (s, 5H), 2.32 (s, 5H), 5.52 (s, 1H), 7.43 (s, 1H), 7.59 (m, 1H), 7.90 (d, 1H), 8.54 (d, 1H), 9.19 (s, 1H).

HPLC-MS: Rt 4.212; m/z 351.1 (MH+).

Example J41: Pyridin-4-ylmethyl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.85 (m, 3H) 1.09 (t, 1H), 2.16 (s, 3H), 2.34 (s, 3H), 2.73 (m, 1H), 5.10 (d, 2H), 5.45 (s, 1H), 7.05 (d, 2H), 7.24 (m, 1H), 7.31 (s, 1H), 7.54 (d, 1H), 8.06 (m, 2H), 8.40 (d, 2H).

HPLC-MS: Rt 5.143; m/z 523.1 (MH+).

Example J42: 4-fluorobenzyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.80 (m, 8H), 1.05 (d, 3H), 2.15 (s, 7H), 2.33 (s, 8H), 5.04 (q, 5H), 5.39 (d, 2H), 7.08 (t, 4H), 7.24 (m, 7H), 7.30 (dd, 3H), 7.99 (s, 2H), 8.29 (dd, 2H), 8.48 (dd, 2H).

HPLC-MS: Rt 5.056; m/z 463.1 (MH+).

A second reaction product was isolated by column chromatography:

Example J42-a: bis(4-fluorobenzyl) 2,6-dicyclopropyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.57 (dd, 2H), 0.82 (m, 6H), 1.02 (d, 2H), 5.01 (d, 4H), 5.34 (d, 1H), 7.19 (m, 8H), 8.05 (dd, 2H), 8.46 (m, 2H).

HPLC-MS: Rt 6.223; m/z 599.2 (MH+).

Example J43: 4-cyanobenzyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

1H-NMR (400 MHz, DMSO-d6) δ = 0.83 (m, 3H), 1.09 (m, 1H), 2.16 (s, 3H), 2.34 (s, 3H), 2.73 (m, 1H), 5.14 (dd, 2H), 5.41 (s, 1H), 7.31 (m, 4H), 7.70 (d, 2H), 8.03 (s, 1H), 8.32 (d, 1H), 8.47 (d, 1H).

HPLC-MS: Rt 4.766; m/z 470.1 (MH+).

Method K Example K1: Methyl 5-acetyl-2-amino-4-(benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate hydrochloride

To a mixture of methyl 5-acetyl-2-amino-4-(benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate (0.07 g, 0,20 mmol) in DCM/MeOH (1:1, 2 mL), 2M HCl in ether (0.21 mL, 0.40 mmol) was added dropwise at 0°C. The mixture was allowed to warm up to RT and stirred at RT overnight. The solvent was removed under reduced pressure, and the remaining solid was washed with ether and dichloromethane affording a white solid (0.055 g, 71 %). Anal. calculated C18H19ClN2O3S (378.08): C, 57.06; H, 5.06; N, 7.39; S, 8.46. Found: C, 56,14; H,5,25; N7.05; S,8.22.
1H-NMR (300 MHz, DMSO-d6) δ = 12.36 (s, 1H), 10.27 (s, 1H), 9.60 (s, 1H), 8.06 (d, J = 8.0 Hz, 1H), 7.90 (d, J = 8.0 Hz, 1H), 7.63 - 7.40 (m, 2H), 7.40 - 7.14 (m, 1H), 4.98 (s, 1H), 4.34 (s, 1H), 3.81 (s, 3H), 2.44 (s, 3H), 2.17 (s, 3H).

Example K2: Methyl 5-acetyl-2-amino-4-(benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate trifluoromethanesulfonate

Method K yielded the title compound as a white solid (0.091 g, 89 %). Anal. calculated C19H19F3N2O6S2 (492.06): C, 46.34; H, 3.89; N, 5.69; S, 13.02. Found: C, 46.28; H, 4.21; N, 5.53; S, 13.15.
1H-NMR (300 MHz, DMSO-d6) δ = 11.67 (s, 1H), 10.04 (s, 1H), 9.34 (s, 1H), 8.06 (d, J = 8.0 Hz, 1H), 7.92 (d, J = 7.9 Hz, 1H), 7.65 - 7.46 (m, 2H), 7.41 - 7.17 (m, 1H), 4.99 (s, 1H), 4.24 (s, 1H), 3.81 (s, 3H), 2.42 (s, 3H), 2.17 (s, 3H).

Method L Example L1: 4-(pyridin-4-yl)benzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4 dihydropyridine-3-carboxylate

A mixture of 4-bromobenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate (0.030 g, 0.06 mmol), pyridin-4-ylboronic acid (0.011 g, 0.09 mmol), Palladium-tetrakis(triphenylphosphine) (0.003 g, 0.003 mmol) and potassium carbonate (0.019 g, 0.18 mmol) in DME:H2O (3:1, 1 mL) was heated at 110°C for 3h. The mixture was allowed to cool to RT, filtered over Celite and the solvent was removed under reduced pressure. The residue was purified by column chromatography (dichloromethane: methanol 2%) affording a yellow solid (0.036 g, 61%).
1H-NMR (300 MHz, CDCl3) δ = 8.67 (s, 2H), 8.45 (dd, J = 4.5, 1.6 Hz, 1H), 8.32 (dd, J = 8.2, 1.6 Hz, 1H), 7.54 - 7.47 (m, 4H), 7.25 - 7.17 (m, 3H), 7.13 (dd, J = 8.2, 4.6 Hz, 1H), 6.48 (bs, 1H), 5.48 (s, 1H), 5.09 (q, J = 15.0 Hz, 2H), 2.36 (s, 6H), 2.16 (s, 3H).
HRMS (IE) m/z calculated C29H25N3O3S [M+]: 495.1617, found: 495.1636.

The following Examples were prepared following Method L.

Example L2: 4-(pyridin-3-yl)benzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.025 g, 51%).

1H-NMR (300 MHz, CDCl3) δ = 8.83 (s, 1H), 8.65 - 8.54 (m, 1H), 8.45 (dd, J = 4.6, 1.6 Hz, 1H), 8.33 (dd, J = 8.2, 1.6 Hz, 1H), 7.87 (dt, J = 8.0, 1.8 Hz, 1H), 7.50 - 7.43 (m, 2H), 7.38 (dd, J = 7.9, 4.8 Hz, 1H), 7.26 - 7.20 (m, 2H), 7.18 (s, 1H), 7.15 (dd, J = 8.2, 4.6 Hz, 1H), 6.61 (s, 1H), 5.48 (s, 1H), 5.12 (q, J = 15.0 Hz, 2H), 2.36 (s, 3H), 2.36 (s, 3H), 2.16 (s, 3H).

HRMS (IE) m/z calculated C29H25N3O3S [M+]: 495.1617, found: 495.1619.

Example L3: 3-(pyridin-4-yl)benzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.037 g, 78%).

1H-NMR (300 MHz, CDCl3) δ = 8.65 (d, J = 4.8 Hz, 2H), 8.42 (dd, J = 4.8, 1.6 Hz, 1H), 8.31 (dd, J = 8.2, 1.6 Hz, 1H), 7.55 (dt, J = 7.7, 1.5 Hz, 1H), 7.46 - 7.33 (m, 4H), 7.22 (dt, J = 7.7, 1.5 Hz, 1H), 7.17 (s, 1H), 7.14 - 7.05 (m, 1H), 6.76 (s, 1H), 5.48 (s, 1H), 5.23 - 5.04 (m, 2H), 2.36 (s, 3H), 2.35 (s, 3H), 2.15 (s, 3H).

HRMS (IE) m/z calculated C29H25N3O3S [M+]: 495.1566, found: 495.1541.

Example L4: 3-(pyridin-3-yl)benzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Yellow solid, 0.26 g, 62%).

1H-NMR (300 MHz, CDCl3) δ = 8.77 (s, 1H), 8.60 (d, J = 4.9 Hz, 1H), 8.42 (dd, J = 4.9, 1.6 Hz, 1H), 8.31 (dd, J = 8.2, 1.6 Hz, 1H), 7.77 (dt, J = 7.9, 1.9 Hz, 1H), 7.49 (dt, J = 7.7, 1.5 Hz, 1H), 7.43 - 7.32 (m, 3H), 7.20 (dt, J = 7.5, 1.5 Hz, 1H), 7.17 (s, 1H), 7.10 (dd, J = 8.2, 4.5 Hz, 1H), 6.62 (s, 1H), 5.47 (s, 1H), 5.15 (q, J = 12.5 Hz, 2H), 2.36 (s, 3H), 2.35 (s, 3H), 2.14 (s, 3H).

HRMS (IE) m/z calculated C29H25N3O3S [M+]: 495.1566, found: 495.1565.

Example L5: 2-(pyridin-4-yl)benzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.023 g, 45%).

1H-NMR (300 MHz, CDCl3) δ = 8.75 (s, 1H), 8.52 (s, 1H), 8.41 (dd, J = 4.6, 1.6 Hz, 1H), 8.13 (dd, J = 8.2, 1.6 Hz, 1H), 7.54 (d, J = 5.3 Hz, 1H), 7.48 - 7.31 (m, 2H), 7.19 (dd, J = 7.3, 1.6 Hz, 1H), 7.13 (s, 1H), 7.08 - 6.94 (m, 3H), 6.37 (s, 1H), 5.36 (s, 1H), 4.97 (q, J = 7.3 Hz, 2H), 2.35 (s, 3H), 2.29 (s, 3H), 2.15 (s, 3H).

HRMS (IE) m/z calculated C29H26N3O3S [M+1]: 496.1689, found: 496.1689.

Example L6: 2-(pyridin-3-yl)benzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.018 g, 48%).

1H-NMR (300 MHz, CDCl3) δ = 8.53 (s, 1H), 8.42 (dd, J = 4.7, 1.6 Hz, 2H), 8.16 (dd, J = 8.2, 1.6 Hz, 1H), 7.45 - 7.27 (m, 4H), 7.25 - 7.16 (m, 2H), 7.14 (s, 1H), 7.09 - 6.94 (m, 1H), 6.42 (s, 1H), 5.38 (s, 1H), 4.97 (q, J = 12 Hz, 2H), 2.35 (s, 3H), 2.28 (s, 3H), 2.15 (s, 3H).

HRMS (IE) m/z calculated C29H26N3O3S [M+1]: 496.1689, found: 496.1687.

Example L7: [3,4'-bipyridin]-5-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.025 g, 64%).

1H-NMR (300 MHz, CDCl3) δ = 8.81 - 8.68 (m, 3H), 8.58 - 8.52 (m, 1H), 8.42 (dd, J = 4.6, 1.6 Hz, 1H), 8.32 (dd, J = 8.2, 1.6 Hz, 1H), 7.60 (t, J = 2.2 Hz, 1H), 7.37 (d, J = 5.2 Hz, 2H), 7.17 (s, 1H), 7.14 (dd, J = 8.2, 4.6 Hz, 1H), 6.38 (s, 1H), 5.46 (s, 1H), 5.17 (q, J = 12 Hz, 2H), 2.36 (s, 6H), 2.16 (s, 3H).

HRMS (IE) m/z calculated C28H24N4O3S [M+]: 496.1569, found: 496.1556.

Example L8: [3,3'-bipyridin]-5-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.022 g, 57%).

1H-NMR (300 MHz, CDCl3) δ = 8.77 - 8.62 (m, 3H), 8.55 - 8.50 (m, 1H), 8.41 (dd, J = 4.6, 1.6 Hz, 1H), 8.33 (dd, J = 8.2, 1.6 Hz, 1H), 7.73 (dt, J = 8.1, 1.8 Hz, 1H), 7.56 (t, J = 2.1 Hz, 1H), 7.43 (dd, J = 8.0, 4.8 Hz, 1H), 7.17 (s, 1H), 7.14 (dd, J = 8.2, 4.6 Hz, 1H), 6.54 (s, 1H), 5.45 (s, 1H), 5.26 - 5.04 (m, 2H), 2.36 (s, 6H), 2.15 (s, 3H).

HRMS (IE) m/z calculated C28H24N4O3S [M+]: 496.1569, found: 496.1580.

Example L9: [2,4'-bipyridin]-5-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.014 g, 23%).

1H-NMR (300 MHz, CDCl3) δ = 8.73 (d, J = 5.1 Hz, 2H), 8.59 (d, J = 2.2 Hz, 1H), 8.44 (dd, J = 4.6, 1.6 Hz, 1H), 8.31 (dd, J = 8.2, 1.6 Hz, 1H), 7.92 - 7.86 (m, 2H), 7.61 (d, J = 8.1 Hz, 1H), 7.40 (dd, J = 8.1, 2.2 Hz, 1H), 7.18 (s, 1H), 7.14 (dd, J = 8.2, 4.6 Hz, 1H), 6.38 (s, 1H), 5.45 (s, 1H), 5.26 - 5.01 (q, J = 15 Hz, 2H), 2.36 (s, 6H), 2.16 (s, 3H).

HRMS (IE) m/z calculated C28H24N4O3S [M+]: 496.1569, found: 496.1563.

Example L10: [2,3'-bipyridin]-5-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.032 g, 72%).

1H-NMR (300 MHz, CDCl3) δ = 9.19 (s, 1H), 8.78 - 8.63 (m, 1H), 8.59 (d, J = 2.3 Hz, 1H), 8.45 (dd, J = 4.6, 1.6 Hz, 1H), 8.31 (dt, J = 8.0, 1.6 Hz, 2H), 7.59 (dd, J = 8.0, 0.9 Hz, 1H), 7.47 - 7.36 (m, 2H), 7.18 (s, 1H), 7.17 - 7.11 (m, 1H), 6.31 (s, 1H), 5.45 (s, 1H), 5.22 - 5.04 (q, J = 12 Hz, 2H), 2.36 (s, 6H), 2.15 (s, 3H).

HRMS (IE) m/z calculated C28H24N4O3S [M+]: 496.1569, found: 496.1563.

Example L11: [2,3'-bipyridin]-4-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.040 g, 82%).

1H-NMR (300 MHz, CDCl3) δ = 9.11 - 9.01 (m, 1H), 8.64 (dd, J = 5.0, 1.5 Hz, 1H), 8.56 (dd, J = 5.0, 0.9 Hz, 1H), 8.43 (dt, J = 4.6, 1.2 Hz, 1H), 8.36 (dt, J = 8.2, 1.2 Hz, 1H), 8.16 (dq, J = 8.0, 1.5, 1.0 Hz, 1H), 7.46 - 7.37 (m, 2H), 7.21 (s, 1H), 7.15 (ddd, J = 8.2, 4.6, 0.9 Hz, 1H), 6.96 (dt, J = 5.1, 1.2 Hz, 1H), 6.76 (s, 1H), 5.51 (s, 1H), 5.27 - 5.03 (q, J = 15 Hz, 2H), 2.38 (d, J = 0.9 Hz, 3H), 2.37 - 2.35 (m, 3H), 2.17 (d, J = 1.0 Hz, 3H).

HRMS (IE) m/z calculated C28H24N4O3S [M+]: 496.1569, found: 496.1570.

Example L12: [2,4'-bipyridin]-4-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Yellow solid (0.024 g, 50%).

1H-NMR (300 MHz, CDCl3) δ = 8.78 - 8.62 (m, 2H), 8.57 (d, J = 5.0 Hz, 1H), 8.44 (dd, J = 4.6, 1.6 Hz, 1H), 8.36 (dd, J = 8.2, 1.6 Hz, 1H), 7.84 - 7.66 (m, 2H), 7.45 (s, 1H), 7.21 (s, 1H), 7.15 (dd, J = 8.2, 4.6 Hz, 1H), 7.00 (dd, J = 5.0, 1.5 Hz, 1H), 6.60 (s, 1H), 5.52 (s, 1H), 5.12 (q, J = 12 Hz, 2H), 2.39 (s, 3H), 2.37 (s, 3H), 2.19 (s, 3H).

HRMS (IE) m/z calculated C28H24N4O3S [M+]: 496.1569, found: 496.1545.

Example L13: Methyl 5-acetyl-2-cyclopropyl-6-methyl-4-(7-(pyridin-4-yl)benzo[b]thiophen-3-yl)-1,4-dihydropyridine-3-carboxylate

Method L in dioxane/water 3:1 gave the title compound as a yellow solid (0.018 g, 64%).
1H-NMR (300 MHz, CDCl3) δ = 8.72 (bs, 2H), 8.21 (dd, J = 8.2, 1.1 Hz, 1H), 7.69 - 7.59 (m, 2H), 7.53 (t, J = 7.7 Hz, 1H), 7.42 - 7.35 (m, 1H), 7.15 (s, 1H), 5.72 (bs, 1H), 5.52 (s, 1H), 3.69 (s, 3H), 2.61 (tt, J = 8.6, 5.7 Hz, 1H), 2.37 (s, 3H), 2.16 (s, 3H), 0.98 (dtq, J = 17.6, 8.6, 4.1 Hz, 2H), 0.79 - 0.56 (m, 2H).
HRMS (IE) m/z calculated C26H24N2O3S [M+]: 444.1508, found: 444.1526.

Example L14: Methyl 5-acetyl-2-cyclopropyl-4-(7-cyclopropylbenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate

Method L in toluene/water 19:1 gave the title compound as a yellow solid (0.030 g, 66%).
1H-NMR (300 MHz, CDCl3) δ = 7.92 (d, J = 8.1 Hz, 1H), 7.32 (t, J = 7.7 Hz, 1H), 7.11 (s, 1H), 6.96 (d, J = 7.7 Hz, 1H), 5.63 (bs, 1H), 5.47 (s, 1H), 3.67 (d, J = 1.4 Hz, 3H), 2.59 (tt, J = 8.6, 5.7 Hz, 1H), 2.36 (s, 3H), 2.13 (s, 3H), 2.11 - 2.03 (m, 1H), 1.07 - 0.86 (m, 4H), 0.85 - 0.75 (m, 2H), 0.73 - 0.57 (m, 2H).
HRMS (IE) m/z calculated C24H25NO3S [M+]: 407.1555, found: 407.1551.

Example L15: 3-(pyridin-4-yl)benzyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate

Method L gave the title compound as a yellow solid (0.018 g, 42 %).
1H-NMR (300 MHz, CDCl3) δ (ppm): 8.66 (d, J = 4.7 Hz, 2H), 8.44 (dd, J = 4.7, 1.4 Hz, 1H), 8.29 (dd, J = 8.2, 1.4 Hz, 1H), 7.56 (d, J = 7.8 Hz, 1H), 7.46 (s, 1H), 7.44 - 7.35 (m, 3H), 7.23 (d, J = 1.4 Hz, 1H), 7.14 - 7.06 (m, 2H), 5.79 (bs, 1H), 5.49 (s, 1H), 5.24 - 5.10 (m, 2H), 2.71 - 2.55 (m, 1H), 2.35 (s, 3H), 2.14 (s, 3H), 1.01 - 0.84 (m, 2H), 0.80 - 0.55 (m, 2H).
HRMS (IE) m/z calculated C31H27N3O3S [M+]: 521.1773, found: 521.1783.
HPLC (95.0%): Rt 31.35 min

Method M Example M1: 1,1'-(2,6-dimethyl-4-(5-(pyridin-3-yl)benzo[b]thiophen-3-yl)-1,4-dihydropyridine-3,5-diyl)diethanone

To a mixture of 1,1'-(4-(5-bromobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-diyl)diethanone (53 mg, 0.13 mmol), 3-pyridylboronic acid (24.8 mg, 0.20 mmol) and [1,1'-bis(diphenylphosphino)ferrocene]dichloropalladium(II) complex with dichloromethane (4.6 mg, 0.0056 mmol) in dry DCM (3 mL) under nitrogen atmosphere at RT, aq 2M Cs2CO3 (0.40 mmol) was added dropwise. The mixture was then refluxed for 24h under a N2 stream, cooled to RT, filtered to remove the catalyst and concentrated in vacuum. Column chromatography afforded the title compound.
1H-NMR (400 MHz, DMSO-d6) δ = 2.20 (s, 6H), 2.32 (s, 6H), 5.60 (s, 1H), 7.30 (s, 1H), 7.56 (dd, 1H), 7.68 (dd, 1H), 8.02 (d, 1H), 8.13 (dd, 1H), 8.52 (d, 1H), 8.61 (s, 1H), 8.97 (s, 1H), 9.17 (s, 1H).
HPLC-MS: Rt 3.645 min, m/z 403.1 (MH+).

Method N Example N1: Methyl 5-acetyl-2-cyclopropyl-6-methyl-4-(7-phenylbenzo[b]thiophen-3-yl)-1,4-dihydropyridine-3-carboxylate

A mixture of methyl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate (0.050 g, 0.11 mmol), tributyl(phenyl) stannane (0.073 mL, 0.22 mmol) and palladium(II)bis(triphenylphosphine)dichloride (0.008 g, 0.011 mmol) in toluene (2 mL) was heated at 90°C for 12 h. The mixture was allowed to cool to RT, filtered over Celite, concentrated and purified by column chromatography (5:1 hexane: ethyl acetate) affording a yellow solid (0.025 g, 50%).
1H-NMR (300 MHz, CDCl3) δ = 8.15 - 8.06 (m, 1H), 7.72 - 7.64 (m, 2H), 7.53 - 7.29 (m, 5H), 7.12 (s, 1H), 5.62 (s, 1H), 5.51 (s, 1H), 3.70 (s, 3H), 2.61 (tt, J = 8.7, 5.7 Hz, 1H), 2.37 (s, 3H), 2.17 (s, 3H), 1.03 - 0.84 (m, 2H), 0.74 - 0.57 (m, 2H).
HRMS (IE) m/z calculated C27H25NO3S [M+]: 443.1555, found: 443.1557.

ABBREVIATIONS

The followiing abbreviations have been used along the present specification:

ACN:
Acetonitrile
Ac:
Acetyl
Dba:
dibenzylideneacetone
DCM:
Dichloromethane
DIBAL:
Diisobutylaluminium hydride
DIPEA:
N,N-Diisopropylethylamine
DMAP:
4-Dimethylaminopyridine
DME:
1,2-Dimethoxyethane
DMEM:
Dulbecco's Modified Eagle's Medium
DMF:
Dimethylformamide
DMSO:
Dimethylsulfoxide
dppf:
1,1'-bis(diphenylphosphino)ferrocene
EDTA:
Ethylenediaminetetraacetic acid
ESI:
Electrospray ionization
EtOH:
Ethanol
FBS:
Fetal bovine serum
HBSS:
Hank's Balanced Solution
HOBT:
Benzotriazol-1-ol
HPLC:
High performance liquid chromatography
HRMS:
High ressolution mass spectrometry
IE:
Electron ionization
i-PrOH:
Isopropanol
MeOH:
Methanol
MS4Å:
4Å Molecular sieves
MW:
molecular weight
NMP:
N-methylpyrrolidone
NMR:
Nuclear magnetic resonance
PEI:
Polyethyleneimine
Ph:
Phenyl
PPA:
Polyphosphoric acid
RT:
Room temperature
Rt:
Retention time
tBut:
ter-butyl
TES:
N-[tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid
TFA:
Trifluoroacetic acid
THF:
Tetrahydrofurane

 A compound of formula (I): wherein: - R1 is a group selected from: a) -COR5, b) -COOR5, c) -CN, d) -C(O)NH2 - R5 is a group selected from: a) linear or branched C1-C6 alkyl optionally substituted by 1, 2 or 3 substituents selected from -N(R6)R7 and -OR6, halogen atom, C3-C6 cycloalkyl and alkynyl group, b) C3-C6 cycloalkyl, - R2 is a group selected from: a) -COOR8, b) -COR8, c) -C(O)N(R8)R9, d) -CN, e) -S(O)nR8, wherein n is an integer from 1 to 2, - R8 and R9 are independently selected from: a) linear or branched C1-C6 alkyl optionally substituted by 1, 2 or 3 substituents selected from A1 or B2, b) A1 group, c) hydrogen atom,
or - R8 and R9 together with the nitrogen atom to which they are attached form a 5-6 membered heterocycle which optionally comprises 1 heteroatom selected from O and N, and said heterocycle being optionally substituted by 1 or 2 groups independently selected from linear or branched C1-C4 alkyl - R3 is a group selected from: a) linear or branched C1-C6 alkyl optionally substituted by 1, 2 or 3 substituents selected from halogen atom, - N(R6)R7, and -OR6, b) C3-C6 cycloalkyl optionally substituted by 1, 2 or 3 halogen atoms, c) hydrogen atom, d) -NH2, e) -CN, - R4 is a group selected from: a) A1 group, b) linear or branched C1-C6 alkyl optionally substituted by 1, 2 or 3 substituents selected from A1 or B2, c) -N(R6)R7, d) -CN, e) -CO-H; f) -CO-Me and g) CO-OMe h) hydrogen atom, - X1, X2, X3, X4, and X5 are independently selected from C-B1, N and C-H, - A1 is selected from: a) C3-C6 cycloalkyl which ring is optionally substituted by 1, 2, 3 or 4 substituents selected from =O and B3; b) a 3 to 6 membered saturated heterocyclyl ring comprising 1, 2 or 3 heteroatoms selected from O, S and N, and which ring is optionally substituted by 1, 2 or 3 substituents selected from =O and B3; c) phenyl or 5 to 6 membered heteroaryl group, either ones are optionally substituted by 1, 2 or 3 substituents selected from B1; - each B1 is independently selected from halogen atom, -CF3 group, 5 to 6 membered heteroaryl, linear or branched C1-C6 alkyl, -CN, -N(R6)R7, -OR6, -C(=O)R6, -C(=O)OR6, -C(=O)N(R6)R7, -OC(=O)-R6, -N(R6)C(=O)R7, -NR7SO2R6, -SO2N(R6)R7, -SR6, -S(O)R6 and -S(O)2R6, - each B2 is independently selected from halogen atom, -CN, -N(R6)R7, -OR6, -C(=O)R6, -C(=O)OR6, -C(=O)N(R6)R7, -OC(=O)-R6, -N(R6)C(=O)R7, -NR7SO2R6, -SO2N(R6)R7, -SR6, -S(O)R6, -S(O)2R6, and alkynyl group,- each B3 is independently selected from halogen atom, linear or branched C1-C6 alkyl, -CN, -N(R6)R7, -OR6, -C(=O)R6, -C(=O)OR6, -C(=O)N(R6)R7, -OC(=O)-R6, -N(R6)C(=O)R7, -NR7SO2R6, -SO2N(R6)R7, -SR6, -S(O)R6, -S(O)2R6, each R6 and R7 independently represents: - hydrogen atom, - linear or branched C1-C12 alkyl, C3-C6 cycloalkyl and C4-C6 heterocycloalkyl, which are optionally substituted by 1, 2 or 3 substituents selected from =O (oxo), halogen atom, hydroxy, phenyl, C3-C6 cycloalkyl, linear or branched C1-C6 alkoxy, amino, alkylamino, dialkylamino, linear or branched C1-C6 alkylcarbonyl, - phenyl or 5 to 6 membered heteroaryl group, which are optionally substituted by 1, 2 or 3 substituents selected from halogen atom, cyano group, linear or branched C1-C6 alkyl, linear or branched C1-C6 haloalkyl, hydroxy, linear or branched C1-C6 alkoxy, amino, alkylamino, dialkylamino; - R6 and R7 form together with the nitrogen atom to which they are attached, a 3-to 8 membered ring which optionally contains a further heteroatom selected from O, N and S, and which ring is optionally substituted by 1, 2 or 3 substituents selected from =O (oxo), linear or branched C1-C6 alkyl, linear or branched C1-C6 haloalkyl, linear or branched C1-C6 alkylcarbonyl; - with the proviso that when R1 is -COOR5 and R2 is -COOR8 then R4 is not a methyl group, and pharmaceutically acceptable salts thereof. Compound according to claim 1 wherein X1, X2, X3 and X5 represent C-H or C-B1, wherein B1 represents halogen atom. Compound according to claim 2 wherein X4 is a group selected from C-B1 and N. Compound according to claim 3 wherein B1 is selected from -CN group and halogen atom. Compound according to any one of claims 2 to 4 wherein R1 is a group selected from -COR5, -COOR5 and -CN group. Compound according to claim 5 wherein R5 is selected from: - C1-C4 alkyl wherein the terminal methyl is unsubstituted or substituted by three fluorine atoms (-CF3) - C1-C3 alkyl optionally substituted at any position by an alkynyl group, and - C3-C5 cycloalkyl, Compound according to any one of claims 2 to 6 wherein R2 represents -COOR8. Compound according to claim 7 wherein R8 represents independently: - linear or branched C1-C6 alkyl optionally substituted by 1, 2 or 3 substituents selected from fluorine atoms and C3-C5 cycloalkyl optionally substituted by 1, 2 or 3 fluorine atoms, or - A1 group, which represents C3-C6 cycloalkyl which is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of fluorine atoms and C1-C3 alkyl groups. Compound according to any one of claims 2 to 8 wherein R3 is a group selected from linear or branched C1-C4 alkyl and C3-C4 cycloalkyl, said groups being optionally substituted by 1, 2 or 3 fluorine atoms. Compound according to any one of claims 2 to 9 wherein R4 is a group selected from: - -N(R6)R7, wherein R6 and R7 are independently selected from a hydrogen atom and a linear or branched C1-C3 alkyl, - A1 group, which represents C3-C6 cycloalkyl, - linear or branched C1-C3 alkyl optionally substituted by 1, 2 or 3 substituents selected from 1, 2 or 3 fluorine atoms or 1 hydroxyl group. Compound according to claim 1 wherein X1, X2, X3 and X5 represent -CH, X4 represents C-B1, wherein B1 represents -CN group or bromine atom, R1 is a group selected from -C(O)CH3, -C(O)OCH3, -C(O)OCH2-alkynyl and CN, R2 is a group selected from -C(O)O-lineal or branched C1-C5 alkyl group optionally substituted by 1, 2 or 3 fluorine atoms and C(O)OCH2-cyclopropyl optionally substituted by 1, 2 or 3 fluorine atoms, R3 is a group selected from linear or branched C1-C3 alkyl and C3-C4 cycloalkyl and R4 is a group selected from a linear or branched C1-C3 alkyl, C3-C4 cycloalkyl and -NH2. Compound according to claim 1 wherein X1, X2, X3 and X5 represent a -CH, X4 represents C-B1, wherein B1 represents -CN group, R1 is a group selected from - C(O)CH3, -C(O)OCH3, R2 is a group selected from -C(O)OCH2-cyclopropyl optionally substituted by 1, 2 or 3 fluorine atoms, and -C(O)OCH2-CF3, R3 is a group selected from methyl and cyclopropyl and R4 is a group selected from cyclopropyl and -NH2. A compound according to claim 1, which is selected from the group consisting of: Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydro-pyridine-3-carboxylate Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydro-pyridine-3-carboxylate (Enantiomer 1) Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydro-pyridine-3-carboxylate (Enantiomer 2) 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxamide 1-(4-(benzo[b]thiophen-3-yl)-5-benzoyl-2,6-dimethyl-1,4-dihydropyridin-3-yl)ethan-1-one Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-methyl-2-phenyl-1,4-dihydropyridine-3-carboxylate 1-(4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-5-nicotinoyl-1,4-dihydro pyridin-3-yl)ethan-1-one Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydro-[2,3'-bipyridine]-3-carboxylate 2,2,2-trifluoroethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate 5-Acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylic acid 1-(4-(Benzo[b]thiophen-3-yl)-2,6-dimethyl-5-(4-methylpiperazine-1-carbonyl)-1,4-dihydropyridin-3-yl)ethan-1-one 5-Acetyl-4-(benzo[b]thiophen-3-yl)-N,N-diethyl-2,6-dimethyl-1,4-dihydropyridine-3-carboxamide 1-(4-(Benzo[b]thiophen-3-yl)-2,6-dimethyl-5-(morpholine-4-carbonyl)-1,4-dihydropyridin-3-yl)ethan-1-one 2-Methoxyethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate 3-Acetamidopropyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Benzyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydro-pyridine-3-carboxylate 2-Morpholinoethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate 2-(Dimethylamino)ethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate 2-Acetamidoethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-(methoxymethyl)-6-methyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-((dimethylamino)methyl)-6-methyl-1,4-dihydropyridine-3-carboxylate Pyridin-4-ylmethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate 4-Methoxybenzyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Pyridin-2-ylmethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-methyl-2-(morpholino-methyl)-1,4-dihydropyridine-3-carboxylate Dimethyl 4-(benzo[b]thiophen-3-yl)-2,6-bis(morpholinomethyl)-1,4-dihydropyridine-3,5-dicarboxylate 2-Hydroxyethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate 1-(4-(Benzo[b]thiophen-3-yl)-5-(cyclopropanecarbonyl)-6-cyclopropyl-2-methyl-1,4-dihydropyridin-3-yl)ethan-1-one Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-(1-(tert-butoxycarbonyl) azetidin-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate (1-(tert-Butoxycarbonyl)piperidin-4-yl)methyl 5-acetyl-4-(benzo[b] thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Cyclohexylmethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Methyl 4-(benzo[b]thiophen-3-yl)-5-(cyclopropanecarbonyl)-2,6-dicyclopropyl-1,4-dihydropyridine-3-carboxylate 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-N-phenyl-1,4-dihydropyridine-3-carboxamide Tetrahydro-2H-pyran-4-yl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate (Tetrahydro-2H-pyran-4-yl)methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Cyclohexyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-(2-methoxy-2-oxoethyl)-6-methyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-2,6-dimethyl-4-(2-methylbenzo[b]thiophen-3-yl)-1,4-dihydropyridine-3-carboxylate Cyclopropylmethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-(2-methoxyethyl)-6-methyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-((benzyloxy)methyl)-6-methyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-methyl-2-(phenoxymethyl)-1,4-dihydropyridine-3-carboxylate Phenethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Methyl 4-(benzo[b]thiophen-3-yl)-5-(cyclopropanecarbonyl)-6-cyclopropyl-2-methyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-methyl-2-(trifluoromethyl)-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-benzyl-2-methyl-1,4-dihydropyridine-3-carboxylate Methyl 4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-5-(2-phenylacetyl)-1,4-dihydropyridine-3-carboxylate Methyl 4-(benzo[b]thiophen-3-yl)-5-(2-methoxyacetyl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-(methoxymethyl)-2-methyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-(fluoromethyl)-6-methyl-1,4-dihydropyridine-3-carboxylate Cyclopropylmethyl 5-acetyl-4-(5-fluorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate 1-(tert-Butoxycarbonyl)piperidin-4-yl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Cyclopentylmethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Cyclopropylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate 1-methylpiperidin-4-yl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Cyclopentyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate 4,4-dimethylcyclohexyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Cyclobutyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-4-(5-fluorobenzo[b]thiophen-3-yl)-6-methyl-2-(trifluoromethyl)-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-2,6-dimethyl-4-(thieno[3,2-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-methyl-6-(trifluoromethyl)-1,4-dihydropyridine-3-carboxylate Cyclopropylmethyl 5-acetyl-4-(5-chlorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Cyclohexylmethyl 5-acetyl-4-(5-chlorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Cyclohexylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate Cyclopentylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate Cyclohexyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate Cyclopentyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-4-(5-chlorobenzo[b]thiophen-3-yl)-6-methyl-2-(trifluoromethyl)-1,4-dihydropyridine-3-carboxylate Benzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate (Tetrahydro-2H-pyran-4-yl)methyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate Benzyl 5-acetyl-4-(5-fluorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Benzyl 5-acetyl-4-(5-chlorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate (Tetrahydro-2H-pyran-4-yl)methyl 5-acetyl-4-(5-chlorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate (Tetrahydro-2H-pyran-4-yl)methyl 5-acetyl-4-(5-fluorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Benzyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-methyl-2-(trifluoromethyl)-1,4-dihydropyridine-3-carboxylate Pyridin-4-ylmethyl 5-acetyl-4-(5-fluorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate 4-Fluorobenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate Pyridin-3-ylmethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate 1,1'-(4-(benzo[b]thiophen-3-yl)-2-benzyl-6-methyl-1,4-dihydropyridine-3,5-diyl)bis(ethan-1-one) 1-(5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-yl)-2-phenylethan-1-one Methyl 5-acetyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-2-(trifluoromethyl)-1,4-dihydropyridine-3-carboxylate 1-(2-methyl-5-(piperidine-1-carbonyl)-4-(thieno[2,3-b]pyridin-3-yl)-6-(trifluoromethyl)-1,4-dihydropyridin-3-yl)ethan-1-one 4-(((5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydro-pyridine-3-carbonyl)oxy)methyl)benzoic acid Benzyl 5-acetyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-2-(trifluoromethyl)-1,4-dihydropyridine-3-carboxylate Pyridin-3-ylmethyl 5-acetyl-4-(5-fluorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Pyridin-3-ylmethyl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Pyridin-3-ylmethyl 5-acetyl-4-(7-cyanobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Pyridin-3-ylmethyl 5-acetyl-4-(5-chlorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Pyridin-3-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate Pyridin-4-ylmethyl 5-acetyl-4-(5-chlorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate 4-(Cyclopropylcarbamoyl)benzyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Pyridin-4-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4dihydropyridine-3-carboxylate 4-Bromobenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate 3-Bromobenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate 2-Bromobenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate (3-Fluoropyridin-4-yl)methyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate Pyrimidin-5-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate (5-Bromopyridin-3-yl)methyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate 2-Phenylpropan-2-yl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate 3-cyanobenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate 4-cyanobenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate (6-Chloropyridin-3-yl)methyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate 3-Morpholinobenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate 4,4-Dimethylcyclohexyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate (2-Chloropyridin-4-yl)methyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate Tetrahydro-2H-pyran-4-yl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate 4,4-Difluorocyclohexyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate 5-Acetyl-N-benzyl-N,2,6-trimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxamide Oxetan-3-yl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate Isopropyl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-6-methyl-2-(trifluoromethyl)-1,4-dihydropyridine-3-carboxylate Cyclopropylmethyl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-2-cyclopropyl-6-methyl-4-(7-(2,2,2-trifluoroacetyl) benzo[b]thiophen-3-yl)-1,4-dihydropyridine-3-carboxylate 2-Phenylpropan-2-yl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-2-amino-4-(benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate Methyl 2-amino-4-(benzo[b]thiophen-3-yl)-5-(cyclopropanecarbonyl)-6-methyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-2-amino-4-(5-fluorobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-2-amino-4-(7-bromobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate Cyclopentyl 5-acetyl-2-amino-4-(7-bromobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate Dimethyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate Cyclopentyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate Dimethyl 2,6-diamino-4-(benzo[b]thiophen-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate cyclopropylmethyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate 4,4-difluorocyclohexyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate methyl 2-amino-5-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate 4-fluorobenzyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate methyl 5-acetyl-2-amino-4-(5-fluorothieno[2,3-b]pyridin-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate methyl 2-acetamido-5-acetyl-4-(5-fluorothieno[2,3-b]pyridin-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate cyclopentylmethyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate 3-(cyclopropylmethyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate dimethyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-cyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate 3-(cyclopropylmethyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-cyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate dimethyl 2,6-diamino-4-(7-cyanobenzo[b]thiophen-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate 5-(cyclopropylmethyl) 3-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-cyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate 3-(4-fluorobenzyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate 3-(4-fluorobenzyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-cyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate Cyclopropylmethyl 2-amino-5-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate 4-Fluorobenzyl 2-amino-5-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate Cyclopropylmethyl 6-amino-5-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-1,4-dihydropyridine-3-carboxylate 3-Cyclopentyl 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate Cyclopropylmethyl 5-acetyl-2-amino-4-(6-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate Cyclohexyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate Cyclohexylmethyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate Cyclopropylmethyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-5-(cyclopropanecarbonyl)-6-methyl-1,4-dihydropyridine-3-carboxylate 3-Fluorobenzyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate 3-(Cyclobutylmethyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate 3-((3,3-Difluorocyclobutyl)methyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate Cyclopropylmethyl 2-amino-5-carbamoyl-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate 3-((2,2-Difluorocyclopropyl)methyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate 5-Cyclopropyl 3-(cyclopropylmethyl) 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate 3-((2,2-Difluorocyclopropyl)methyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-cyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate 3-(Cyclopropylmethyl) 5-methyl 2-amino-4-(7-cyano-5-fluorobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate 3-(cyclopropylmethyl) 5-methyl 2-amino-4-(7-cyano-4-fluorobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate 3-Isopropyl 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate 3-((2,2-Difluoro-3,3-dimethylcyclopropyl)methyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate 5-Methyl 3-neopentyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate 3-(Cyclopropylmethyl) 5-methyl 2-amino-4-(7-cyanothieno[3,2-b]pyridin-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate 5-Methyl 3-neopentyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-cyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate Bis(cyclopropylmethyl) 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate 3-(Cyclopropylmethyl) 5-(prop-2-yn-1-yl) 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate 3-(Cyclopropylmethyl) 5-methyl 2-amino-4-(5,7-dicyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate 5-(But-2-yn-1-yl) 3-(cyclopropylmethyl) 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate 5-Methyl 3-(2,2,2-trifluoroethyl) 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate 3-(Cyclopropylmethyl) 5-(2,2,2-trifluoroethyl) 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate 3-(Cyclopropylmethyl) 5-methyl 2-amino-4-(6-chloro-7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate 3-(2-Fluoro-2-methylpropyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate 3-(Cyclopropylmethyl) 5-methyl 2-amino-4-(7-cyano-6-(trifluoromethyl)benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate 5-Methyl 3-prop-2-yn-1-yl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate 3-(Cyclopropylmethyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-(trifluoromethyl)-1,4-dihydropyridine-3,5-dicarboxylate 4-(Benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-dicarbonitrile 4-(6-hydroxybenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-dicarbonitrile 4-(6-methoxybenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-dicarbonitrile 2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3,5-dicarbonitrile 4-(benzo[b]thiophen-3-yl)-2-methyl-6-phenyl-1,4-dihydropyridine-3,5-dicarbonitrile 4-(benzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3,5-dicarbonitrile 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carbonitrile Ethyl 4-(benzo[b]thiophen-3-yl)-5-cyano-2,6-dimethyl-1,4-dihydropyridine -3-carboxylate Ethyl 4-(benzo[b]thiophen-3-yl)-5-cyano-2-methyl-6-phenyl-1,4-dihydropyridine-3-carboxylate Ethyl 4-(benzo[b]thiophen-3-yl)-5-cyano-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate Methyl 4-(benzo[b]thiophen-3-yl)-5-cyano-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate 1,1'-(4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-diyl)diethanone 1,1'-(4-(6-hydroxybenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-diyl)bis(ethan-1-one) 1,1'-(4-(6-Methoxybenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-diyl)diethanone 1,1'-(4-(Benzo[b]thiophen-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridine-3,5-diyl)diethanone 1,1'-(4-(5-chlorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-diyl)diethanone 1,1'-(4-(5-bromobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-diyl)diethanone 1,1'-(4-(5-fluorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-diyl)bis(ethan-1-one) 1,1'-(2,6-dimethyl-4-(5-morpholinobenzo[b]thiophen-3-yl)-1,4-dihydropyridine-3,5-diyl)bis(ethan-1-one) 1,1'-(2,6-dimethyl-4-(5-(4-methylpiperazin-1-yl)benzo[b]thiophen-3-yl)-1,4-dihydropyridine-3,5-diyl)bis(ethan-1-one) 1,1'-(4-(5-(benzylamino)benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-diyl)bis(ethan-1-one) 3-(3,5-diacetyl-2,6-dimethyl-1,4-dihydropyridin-4-yl)benzo[b]thiophene-5-carbonitrile 3-(3,5-diacetyl-2,6-dimethyl-1,4-dihydropyridin-4-yl)benzo[b]thiophene-5-carboxylic acid N-cyclopropyl-3-(3,5-diacetyl-2,6-dimethyl-1,4-dihydropyridin-4-yl)benzo[b]thiophene-5-carboxamide N-(cyclopropylmethyl)-3-(3,5-diacetyl-2,6-dimethyl-1,4-dihydropyridin-4-yl)benzo[b]thiophene-5-carboxamide 1,1'-(2,6-Dimethyl-4-(5-(4-methylpiperazine-1-carbonyl)benzo[b]thiophen-3-yl)-1,4-dihydropyridine-3,5-diyl)bis(ethan-1-one) 1,1'-(2,6-Dimethyl-4-(5-(morpholine-4-carbonyl)benzo[b]thiophen-3-yl)-1,4-dihydropyridine-3,5-diyl)bis(ethan-1-one) 1,1'-(2,6-Dimethyl-4-(thieno[3,2-c]pyridin-3-yl)-1,4-dihydropyridine-3,5-diyl)bis(ethan-1-one) 1,1'-(2,6-Dimethyl-4-(thieno[2,3-c]pyridin-3-yl)-1,4-dihydropyridine-3,5-diyl)bis(ethan-1-one) 1,1'-(2,6-Dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3,5-diyl)bis(ethan-1-one) Dimethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-2,3-dicarboxylate Dimethyl 5-acetyl-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-2,3-dicarboxylate Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate Dimethyl 4-(benzo[b]thiophen-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate Ethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-4-(5-chlorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate 1,1'-(4-(benzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydro-pyridine-3,5-diyl)diethanone 1-(4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-5-(methylsulfonyl)-1,4-dihydro-pyridin-3-yl)ethanone Methyl 5-acetyl-4-(5-fluorobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-4-(5-bromobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate methyl 5-acetyl-4-(5-cyanobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate 3-(3-acetyl-5-(methoxycarbonyl)-2,6-dimethyl-1,4-dihydropyridin-4-yl)benzo[b]thiophene-5-carboxylic acid methyl 5-acetyl-4-(5-(cyclopropylcarbamoyl)benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-4-(5-((cyclopropylmethyl)carbamoyl)benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-2,6-dimethyl-4-(5-(4-methylpiperazine-1-carbonyl)benzo[b]thiophen-3-yl)-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-2,6-dimethyl-4-(5-(morpholine-4-carbonyl)benzo[b]thiophen-3-yl)-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-2,6-dimethyl-4-(thieno[3,2-c]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-c]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-2-cyclopropyl-4-(5-fluorobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate Dimethyl 2,6-dicyclopropyl-4-(5-fluorobenzo[b]thiophen-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate Methyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate Dimethyl 2,6-dicyclopropyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-4-(5-chlorobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate Dimethyl 4-(5-chlorobenzo[b]thiophen-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate Methyl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-2-cyclopropyl-4-(7-(cyclopropylcarbamoyl)benzo[b] thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate Dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate Methyl 5-acetyl-2-cyclopropyl-4-(7-(methoxycarbonyl) benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate Dimethyl 2,6-dicyclopropyl-4-(7-(methoxycarbonyl)benzo[b]thiophen-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate 3-(3-Acetyl-6-cyclopropyl-5-(methoxycarbonyl)-2-methyl-1,4-dihydropyridin-4-yl)benzo[b]thiophene-7-carboxylic acid Benzyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate Pyridin-4-ylmethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate bis(pyridin-4-ylmethyl) 2,6-dicyclopropyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate Pyridin-4-ylmethyl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Pyridin-4-ylmethyl 5-acetyl-4-(5-chlorobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate Pyridin-4-ylmethyl 5-acetyl-4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate Pyridin-4-ylmethyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate Benzyl 5-acetyl-2-cyclopropyl-4-(7-(methoxycarbonyl)benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate Dibenzyl 2,6-dicyclopropyl-4-(7-(methoxycarbonyl)benzo[b]thiophen-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate Benzyl 5-acetyl-4-(7-(methoxycarbonyl)benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate Methyl 3-(3,5-diacetyl-2,6-dimethyl-1,4-dihydropyridin-4-yl)benzo[b]thiophene-7-carboxylate 3-(3,5-Diacetyl-2,6-dimethyl-1,4-dihydropyridin-4-yl)benzo[b]thiophene-7-carbonitrile Pyridin-4-ylmethyl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate 4-Fluorobenzyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate bis(4-fluorobenzyl) 2,6-dicyclopropyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate 4-cyanobenzyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-4-(7-chlorobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate methyl 5-acetyl-2-cyclopropyl-6-methyl-4-(7-(trifluoromethyl)benzo[b]thiophen-3-yl)-1,4-dihydropyridine-3-carboxylate 3-chlorobenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate 2-phenylpropan-2-yl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate Cyclohexyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate 2-Phenylpropan-2-yl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate 1-(4-(7-Bromobenzo[b]thiophen-3-yl)-5-(cyclopropanecarbonyl)-6-cyclopropyl-2-methyl-1,4-dihydropyridin-3-yl)ethan-1-one 3-chlorobenzyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate 2-(4-Fluorophenyl)propan-2-yl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate 2-(4-fluorophenyl)propan-2-yl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate 2-(4-fluorophenyl)propan-2-yl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-2-cyclopropyl-4-(7-fluorobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate Cyclopropylmethyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate Cyclopentyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate Cyclopropylmethyl 5-acetyl-4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2-cyclobutyl-6-methyl-1,4-dihydropyridine-3-carboxylate Dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate Methyl 5-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2-cyclopentyl-6-methyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2-cyclohexyl-6-methyl-1,4-dihydropyridine-3-carboxylate Methyl 5-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridine-3-carboxylate 3-((4-Methylpiperazin-1-yl)methyl)benzyl 5-acetyl-4-(7-bromobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3-carboxylate 3-(cyclopropylmethyl) 5-methyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate 3-(cyclopropylmethyl) 5-methyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate cyclopropylmethyl 5-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate dimethyl 2-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-6-cyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate dimethyl 2-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate methyl 5-acetyl-2-((2-aminoethoxy)methyl)-4-(benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate methyl 5-acetyl-2-((2-aminoethoxy)methyl)-4-(7-bromobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate methyl 5-acetyl-2-((2-aminoethoxy)methyl)-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate Dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-formyl-1,4-dihydropyridine-3,5-dicarboxylate Dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-(hydroxymethyl)-1,4-dihydropyridine-3,5-dicarboxylate Dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-formyl-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate Dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-(hydroxymethyl)-6-methyl-1,4-dihydropyridine-3,5-dicarboxylate Dimethyl 2-cyano-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate Cyclopropylmethyl 5-acetyl-4-(6-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridine-3-carboxylate Cyclopropylmethyl 2,5-diacetyl-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-2-amino-4-(benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate hydrochloride Methyl 5-acetyl-2-amino-4-(benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate trifluoromethanesulfonate 4-(Pyridin-4-yl)benzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4 dihydropyridine-3-carboxylate 4-(Pyridin-3-yl)benzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate 3-(Pyridin-4-yl)benzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate 3-(Pyridin-3-yl)benzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate 2-(Pyridin-4-yl)benzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate 2-(pyridin-3-yl)benzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate [3,4'-Bipyridin]-5-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate [3,3'-Bipyridin]-5-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate [2,4'-Bipyridin]-5-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate [2,3'-Bipyridin]-5-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate [2,3'-Bipyridin]-4-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate [2,4'-Bipyridin]-4-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-2-cyclopropyl-6-methyl-4-(7-(pyridin-4-yl)benzo[b]thiophen-3-yl)-1,4-dihydropyridine-3-carboxylate Methyl 5-acetyl-2-cyclopropyl-4-(7-cyclopropylbenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridine-3-carboxylate 3-(Pyridin-4-yl)benzyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate 1,1'-(2,6-Dimethyl-4-(5-(pyridin-3-yl)benzo[b]thiophen-3-yl)-1,4-dihydro-pyridine-3,5-diyl)diethanone Methyl 5-acetyl-2-cyclopropyl-6-methyl-4-(7-phenylbenzo[b]thiophen-3-yl)-1,4-dihydropyridine-3-carboxyl ate Compound as defined in any one of claims 1 to 13 for use in the treatment of a disease or pathological condition susceptible of improvement by antagonism of androgen receptor and/or glucocorticoid receptor selected from prostate cancer, castration-resistant prostate cancer, pancreatic cancer, bladder cancer, renal cancer, gastric cancer, lung cancer, breast cancer, colon cancer, colorectal cancer, ovarian cancer, and other solid tumours, melanoma, metastasizing cancers, benign prostate hyperplasia, polycystic ovary syndrome (PCOS), hair loss, hirsutism, acne, hypogonadism, muscle wasting diseases and cachexia, and Cushing's syndrome, anti-psychotic drug induced weight gain, obesity, post-traumatic stress disorder and alcoholism. A pharmaceutical composition comprising a compound as defined in any one of claims 1 to 13 and a pharmaceutically acceptable diluent or carrier. A pharmaceutical composition according to claim 15 further comprising a therapeutically effective amount of a therapeutic agent selected from agents for treating prostate cancer, castration-resistant prostate cancer, pancreatic cancer, bladder cancer, renal cancer, gastric cancer, lung cancer, breast cancer, colon cancer, colorectal cancer, ovarian cancer, and other solid tumours, melanoma, metastasizing cancers, benign prostate hyperplasia, polycystic ovary syndrome (PCOS), hair loss, hirsutism, acne, hypogonadism, muscle wasting diseases and cachexia, and Cushing's syndrome, anti-psychotic drug induced weight gain, obesity, post-traumatic stress disorder and alcoholism. A combination product comprising a compound according to any one claims 1 to 13 and a therapeutic agent used for the treatment of prostate cancer, castration-resistant prostate cancer, pancreatic cancer, bladder cancer, renal cancer, gastric cancer, lung cancer, breast cancer, colon cancer, colorectal cancer, ovarian cancer, and other solid tumours, melanoma, metastasizing cancers, benign prostate hyperplasia, polycystic ovary syndrome (PCOS), hair loss, hirsutism, acne, hypogonadism, muscle wasting diseases and cachexia, and Cushing's syndrome, anti-psychotic drug induced weight gain, obesity, post-traumatic stress disorder and alcoholism, in particular a therapeutic agent selected from gonadotropin-releasing hormone (GnRH) receptor agonist or antagonist, androgen receptor antagonist, CYP17 inhibitor, VEGF inhibitor, EGFR inhibitor, PI3K inhibitor, AKT inhibitor, mTOR inhibitor, c-Met inhibitor, Src, inhibitor, PARP inhibitor, angiopoietin, ALK inhibitor, ROS-1 inhibitor, anti-(IGF) antibodies, taxane anti-neoplastic agent, topoisomerase II inhibitor, anti-tumor antibiotic, HSP90 inhibitor, aurora kinase inhibitor, PSA-directed vaccine, GR antagonists, 11-beta HSD inhibitors, one or more immunotherapeutic agent selected from the group consisting of antibodies anti-CTLA4, antibodies anti-PD1 and antibodies anti-PDL1, in particular an antibody selected from the group consisting of ipilimumab, tremelimumab, nivolumab, pembrolizumab, CT-011, AMP-224, MPDL3280A, MEDI4736 and MDX-1105. Eine Verbindung mit der Formel (I): worin: - R1 eine Gruppe ist ausgewählt aus: a) -COR5, b) -COOR5, c) -CN, d) -C(O)NH2 - R5 eine Gruppe ist ausgewählt aus: a) linearem oder verzweigtem C1-C6-Alkyl optional substituiert durch 1, 2 oder 3 Substituenten ausgewählt aus -N(R6)R7 und - OR6, Halogenatom, C3-C6-Cycloalkyl und Alkynylgruppe, b) C3-C6-Cycloalkyl, - R2 eine Gruppe ist ausgewählt aus: a) -COOR8, b) -COR8, c) -C(O)N(R8)R9, d) -CN, e) -S(O)nR8, wobei n eine ganze Zahl von 1 bis 2 ist, - R8 und R9 unabhängig voneinander ausgewählt sind: a) linearem oder verzweigtem C1-C6-Alkyl optional substituiert durch 1, 2 oder 3 Substituenten ausgewählt aus A1 oder B2, b) A1-Gruppe, c) Wasserstoffatom,
oder - R8 und R9 zusammen mit dem Stickstoffatom, an das sie gebunden sind, einen 5- bis 6-gliedrigen Heterozcyklus bilden, der optional 1 Heteroatom ausgewählt aus O und N enthält, und wobei der Heterocyclus optional substituiert ist durch 1 oder 2 Gruppen unabhängig voneinander ausgewählt aus linearem oder verzweigtem C1-C4-Alkyl - R3 eine Gruppe ist ausgewählt aus: a) linearem oder verzweigtem C1-C6-Alkyl optional substituiert durch 1, 2 oder 3 Substituenten ausgewählt aus Halogenatom, - N(R6)R7, und -OR6, b) C3-C6-Cycloalkyl optional substituiert durch 1, 2 oder 3 Halogenatome, c) Wasserstoffatom, d) -NH2, e) -CN, - R4 eine Gruppe ist ausgewählt aus: a) A1-Gruppe, b) linearem oder verzweigtem C1-C6-Alkyl optional substituiert durch 1, 2 oder 3 Substituenten ausgewählt aus A1 oder B2, c) -N(R6)R7, d) -CN, e) -CO-H; f) -CO-Me und g) CO-OMe h) Wasserstoffatom, - X1, X2, X3, X4, und X5 unabhängig voneinander ausgewählt aus C-B1, N und C-H, - A1 ausgewählt ist aus: a) C3-C6-Cycloalkyl dessen Ring optional substituiert ist durch 1, 2, 3 oder 4 Substituenten ausgewählt aus =O und B3; b) einem 3- bis 6-gliedrigen gesättigten Heterocyclylring enthaltend 1, 2 oder 3 Heteroatome ausgewählt aus O, S und N, und wobei der Ring optional substituiert ist durch 1, 2 oder 3 Substituenten ausgewählt aus =O und B3; c) Phenyl oder einer 5- bis 6-gliedrigen Heteroarylgruppe, wobei beide Gruppen optional substituiert sind durch 1, 2 oder 3 Substituenten ausgewählt aus B1; - wobei jedes B1 unabhängig ausgewählt ist aus Halogenatom, -CF3-Gruppe, 5- bis 6-gliedrigem Heteroaryl, linearem oder verzweigtem C1-C6-Alkyl, -CN, -N(R6)R7, -OR6, -C(=O)R6, -C(=O)OR6, -C(=O)N(R6)R7, -OC(=O)-R6, -N(R6)C(=O)R7, -NR7SO2R6, -SO2N(R6)R7, -SR6, -S(O)R6 und -S(O)2R6, - jedes B2 unabhängig ausgewählt ist aus Halogenatom, -CN, -N(R6)R7, -OR6, -C(=O)R6, -C(=O)OR6, -C(=O)N(R6)R7,-OC(=O)-R6, -N(R6)C(=O)R7, -NR7SO2R6, -SO2N(R6)R7, -SR6, -S(O)R6, -S(O)2R6,und Alkynylgruppe, - jedes B3 unabhängig ausgewählt ist aus Halogenatom, linearem oder verzweigtem C1-C6-Alkyl, -CN, -N(R6)R7, -OR6, -C(=O)R6, -C(=O)OR6, -C(=O)N(R6)R7, -OC(=O)-R6, -N(R6)C(=O)R7, -NR7SO2R6, -SO2N(R6)R7, -SR6, -S(O)R6, -S(O)2R6, jedes R6 und R7 unabhängig voneinander darstellt: - Wasserstoffatom, - lineares oder verzweigtes C1-C12-Alkyl, C3-C6-Cycloalkyl und C4-C6-Heterocycloalkyl, die optional substituiert sind durch 1, 2 oder 3 Substituenten ausgewählt aus =O (Oxo), Halogenatom, Hydroxyl, Phenyl, C3-C6-Cycloalkyl, linearem oder verzweigtem C1-C6-Alkoxy, Amino, Alkylamino, Dialkylamino, linearem oder verzweigtem C1-C6-Alkylcarbonyl, - Phenyl oder 5- bis 6-gliedrige Heteroarylgruppe, die optional substituiert sind durch 1, 2 oder 3 Substituenten ausgewählt aus Halogenatom, Cyanogruppe, linearem oder verzweigtem C1-C6-Alkyl, linearem oder verzweigtem C1-C6-Haloalkyl, Hydroxyl, linearem oder verzweigtem C1-C6-Alkoxy, Amino, Alkylamino, Dialkylamino; - R6 und R7 zusammen mit dem Stickstoffatom, an das sie gebunden sind, einen 3- bis 8-gliedrigen Ring bilden, der optional ein weiteres Heteroatom enthält ausgewählt aus O, N und S, und wobei der Ring optional substituiert ist durch 1, 2 oder 3 Substituentens ausgewählt aus =O (Oxo), linearem oder verzweigtem C1-C6-Alkyl, linearem oder verzweigtem C1-C6-Haloalkyl, linearem oder verzweigtem C1-C6-Alkylcarbonyl; - mit der Maßgabe, dass, wenn R1 -COOR5 ist und R2 -COOR8 ist, dann R4 keine Methylgruppe darstellt. und pharmazeutisch annehmbare Salze davon. Verbindung gemäß Anspruch 1, wobei X1, X2, X3 und X5 C-H oder C-B1 darstellen, wobei B1 Halogenatom darstellt. Verbindung gemäß Anspruch 2, wobei X4 eine Gruppe ist ausgewählt aus C-B1 und N. Verbindung gemäß Anspruch 3, wobei B1 ausgewählt ist aus -CN-Gruppe und Halogenatom. Verbindung gemäß irgendeinem der Ansprüche 2 bis 4, wobei R1 eine Gruppe ist ausgewählt aus -COR5, -COOR5 und -CN-Gruppe. Verbindung gemäß Anspruch 5, wobei R5 ausgewählt ist aus: - C1-C4-Alkyl, wobei das endständige Methyl unsubstituiert ist oder durch drei Fluoratome (-CF3) substituiert ist - C1-C3-Alkyl optional substituiert an irgendeiner Position durch eine Alkynylgruppe, und - C3-C5-Cycloalkyl. Verbindung gemäß irgendeinem der Ansprüche 2 bis 6, wobei R2 - COOR8 darstellt. Verbindung gemäß Anspruch 7, wobei R8 unabhängig darstellt: - lineares oder verzweigtes C1-C6-Alkyl optional substituiert durch 1, 2 oder 3 Substituenten ausgewählt aus Fluoratomen und C3-C5-Cycloalkyl optional substituiert durch 1, 2 oder 3 Fluoratom, oder - A1-Gruppe, die C3-C6-Cycloalkyl darstellt, das optional substituiert ist durch 1, 2 oder 3 Substituenten ausgewählt aus der Gruppe bestehend aus Fluoratomen und C1-C3-Alkylgruppen. Verbindung gemäß irgendeinem der Ansprüche 2 bis 8, wobei R3 eine Gruppe ist ausgewählt aus linearem oder verzweigtem C1-C4-Alkyl und C3-C4-Cycloalkyl, wobei die Gruppen optional substituiert sind durch 1, 2 oder 3 Fluoratome. Verbindung gemäß irgendeinem der Ansprüche 2 bis 9, wobei R4 eine Gruppe ist ausgewählt aus: - -N(R6)R7, wobei R6 und R7 unabhängig voneinander ausgewählt sind aus Wasserstoffatom und einem linearen oder verzweigten C1-C3-Alkyl, - A1-Gruppe, die C3-C6-Cycloalkyl darstellt, - linearem oder verzweigtem C1-C3-Alkyl optional substituiert durch 1, 2 oder 3 Substituenten ausgewählt aus 1, 2 oder 3 Fluoratomen oder 1 Hydroxylgruppe. Verbindung gemäß Anspruch 1, wobei X1, X2, X3 und X5 -CH darstellen, X4 C-B1 darstellt, wobei B1 -CN-Gruppe oder Bromatom darstellt, R1 eine Gruppe ist ausgewählt aus -C(O)CH3, -C(O)OCH3 -C(O)OCH2-Alkynyl und CN, R2 eine Gruppe ist ausgewählt aus -C(O)O-linearer oder verzweigter C1-C5-Alkylgruppe optional substituiert durch 1, 2 oder 3 Fluoratome und C(O)OCH2-Cyclopropyl optional substituiert durch 1, 2 oder 3 Fluoratome, R3 eine Gruppe ist ausgewählt aus linearem oder verzweigtem C1-C3-Alkyl und C3-C4-Cycloalkyl und R4 eine Gruppe ist ausgewählt aus linearem oder verzweigtem C1-C3-Alkyl, C3-C4-Cycloalkyl und -NH2. Verbindung gemäß Anspruch 1, wobei X1, X2, X3 und X5 -CH darstellt, X4 C-B1 darstellt, wobei B1 -CN-Gruppe darstellt, R1 eine Gruppe ist ausgewählt aus -C(O)CH3, -C(O)OCH3, R2 eine Gruppe ist ausgewählt aus -C(O)OCH2-Cyclopropyl optional substituiert durch 1, 2 oder 3 Fluoratome, und -C(O)OCH2-CF3, R3 eine Gruppe ist ausgewählt aus Methyl und Cyclopropyl und R4 eine Gruppe ist ausgewählt aus Cyclopropyl und -NH2. Eine Verbindung gemäß Anspruch 1, die ausgewählt ist aus der Gruppe bestehend aus: Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydro-pyridin-3-carboxylat Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydro-pyridin-3-carboxylat (Enantiomer 1) Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydro-pyridin-3-carboxylat (Enantiomer 2) 5-Acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxamid 1-(4-(Benzo[b]thiophen-3-yl)-5-benzoyl-2,6-dimethyl-1,4-dihydropyridin-3-yl)ethan-1-on Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-methyl-2-phenyl-1,4-dihydropyridin-3-carboxylat 1-(4-(Benzo[b]thiophen-3-yl)-2,6-dimethyl-5-nicotinoyl-1,4-dihydro pyridin-3-yl)ethan-1-on Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydro-[2,3'-bipyridin]-3-carboxylat 2,2,2-Trifluorethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat 5-Acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carbonsäure 1-(4-(Benzo[b]thiophen-3-yl)-2,6-dimethyl-5-(4-methylpiperazin-1-carbonyl)-1,4-dihydropyridin-3-yl)ethan-1-on 5-Acetyl-4-(benzo[b]thiophen-3-yl)-N,N-diethyl-2,6-dimethyl-1,4-dihydropyridin-3-carboxamid 1-(4-(Benzo[b]thiophen-3-yl)-2,6-dimethyl-5-(morpholin-4-carbonyl)-1,4-dihydropyridin-3-yl)ethan-1-on 2-Methoxyethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat 3-Acetamidopropyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Benzyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydro-pyridin-3-carboxylat 2-Morpholinoethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat 2-(Dimethylamino)ethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat 2-Acetamidoethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-(methoxymethyl)-6-methyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-((dimethylamino)methyl)-6-methyl-1,4-dihydropyridin-3-carboxylat Pyridin-4-ylmethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat 4-Methoxybenzyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Pyridin-2-ylmethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-methyl-2-(morpholino-methyl)-1,4-dihydropyridin-3-carboxylat Dimethyl 4-(benzo[b]thiophen-3-yl)-2,6-bis(morpholinomethyl)-1,4-dihydropyridin-3,5-dicarboxylat 2-Hydroxyethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat 1-(4-(Benzo[b]thiophen-3-yl)-5-(cyclopropancarbonyl)-6-cyclopropyl-2-methyl-1,4-dihydropyridin-3-yl)ethan-1-on Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-(1-(tert-butoxycarbonyl) azetidin-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat (1-(tert-Butoxycarbonyl)piperidin-4-yl)methyl 5-acetyl-4-(benzo[b] thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Cyclohexylmethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Methyl 4-(benzo[b]thiophen-3-yl)-5-(cyclopropancarbonyl)-2,6-dicyclopropyl-1,4-dihydropyridin-3-carboxylat 5-Acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-N-phenyl-1,4-dihydropyridin-3-carboxamid Tetrahydro-2H-pyran-4-yl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat (Tetrahydro-2H-pyran-4-yl)methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Cyclohexyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-(2-methoxy-2-oxoethyl)-6-methyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-2,6-dimethyl-4-(2-methylbenzo[b]thiophen-3-yl)-1,4-dihydropyridin-3-carboxylat Cyclopropylmethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-(2-methoxyethyl)-6-methyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-((benzyloxy)methyl)-6-methyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-methyl-2-(phenoxymethyl)-1,4-dihydropyridin-3-carboxylat Phenethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Methyl 4-(benzo[b]thiophen-3-yl)-5-(cyclopropancarbonyl)-6-cyclopropyl-2-methyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-methyl-2-(trifluormethyl)-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-benzyl-2-methyl-1,4-dihydropyridin-3-carboxylat Methyl 4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-5-(2-phenylacetyl)-1,4-dihydropyridin-3-carboxylat Methyl 4-(benzo[b]thiophen-3-yl)-5-(2-methoxyacetyl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-(methoxymethyl)-2-methyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-(fluormethyl)-6-methyl-1,4-dihydropyridin-3-carboxylat Cyclopropylmethyl 5-acetyl-4-(5-fluorbenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat 1-(tert-Butoxycarbonyl)piperidin-4-yl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Cyclopentylmethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Cyclopropylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat 1-Methylpiperidin-4-yl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Cyclopentyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat 4,4-Dimethylcyclohexyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Cyclobutyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(5-fluorbenzo[b]thiophen-3-yl)-6-methyl-2-(trifluormethyl)-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-2,6-dimethyl-4-(thieno[3,2-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-methyl-6-(trifluormethyl)-1,4-dihydropyridin-3-carboxylat Cyclopropylmethyl 5-acetyl-4-(5-chlorbenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Cyclohexylmethyl 5-acetyl-4-(5-chlorbenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Cyclohexylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat Cyclopentylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat Cyclohexyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat Cyclopentyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(5-chlorbenzo[b]thiophen-3-yl)-6-methyl-2-(trifluormethyl)-1,4-dihydropyridin-3-carboxylat Benzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat (Tetrahydro-2H-pyran-4-yl)methyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat Benzyl 5-acetyl-4-(5-fluorbenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Benzyl 5-acetyl-4-(5-chlorbenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat (Tetrahydro-2H-pyran-4-yl)methyl 5-acetyl-4-(5-chlorbenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat (Tetrahydro-2H-pyran-4-yl)methyl 5-acetyl-4-(5-fluorbenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Benzyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-methyl-2-(trifluormethyl)-1,4-dihydropyridin-3-carboxylat Pyridin-4-ylmethyl 5-acetyl-4-(5-fluorbenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat 4-Fluorbenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat Pyridin-3-ylmethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat 1,1'-(4-(Benzo[b]thiophen-3-yl)-2-benzyl-6-methyl-1,4-dihydropyridin-3,5-diyl)bis(ethan-1-on) 1-(5-Acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-yl)-2-phenylethan-1-on Methyl 5-acetyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-2-(trifluormethyl)-1,4-dihydropyridin-3-carboxylat 1-(2-Methyl-5-(piperidin-1-carbonyl)-4-(thieno[2,3-b]pyridin-3-yl)-6-(trifluormethyl)-1,4-dihydropyridin-3-yl)ethan-1-on 4-(((5-Acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydro-pyridin-3-carbonyl)oxy)methyl)benzoesäure Benzyl 5-acetyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-2-(trifluormethyl)-1,4-dihydropyridin-3-carboxylat Pyridin-3-ylmethyl 5-acetyl-4-(5-fluorbenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Pyridin-3-ylmethyl 5-acetyl-4-(7-brombenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Pyridin-3-ylmethyl 5-acetyl-4-(7-cyanobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Pyridin-3-ylmethyl 5-acetyl-4-(5-chlorbenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Pyridin-3-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat Pyridin-4-ylmethyl 5-acetyl-4-(5-chlorbenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat 4-(Cyclopropylcarbamoyl)benzyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Pyridin-4-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat 4-Brombenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat 3-Brombenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat 2-Brombenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat (3-Fluorpyridin-4-yl)methyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat Pyrimidin-5-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat (5-Brompyridin-3-yl)methyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat 2-Phenylpropan-2-yl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat 3-Cyanobenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat 4-Cyanobenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat (6-Chlorpyridin-3-yl)methyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat 3-Morpholinobenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat 4,4-Dimethylcyclohexyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat (2-Chlorpyridin-4-yl)methyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat Tetrahydro-2H-pyran-4-yl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat 4,4-Difluorcyclohexyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat 5-Acetyl-N-benzyl-N,2,6-trimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxamid Oxetan-3-yl 5-acetyl-4-(7-brombenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridin-3-carboxylat Isopropyl 5-acetyl-4-(7-brombenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(7-brombenzo[b]thiophen-3-yl)-6-methyl-2-(trifluormethyl)-1,4-dihydropyridin-3-carboxylat Cyclopropylmethyl 5-acetyl-4-(7-brombenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-2-cyclopropyl-6-methyl-4-(7-(2,2,2-trifluoracetyl) benzo[b]thiophen-3-yl)-1,4-dihydropyridin-3-carboxylat 2-Phenylpropan-2-yl 5-acetyl-4-(7-brombenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-2-amino-4-(benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat Methyl 2-amino-4-(benzo[b]thiophen-3-yl)-5-(cyclopropancarbonyl)-6-methyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-2-amino-4-(5-fluorbenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-2-amino-4-(7-brombenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat Cyclopentyl 5-acetyl-2-amino-4-(7-brombenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat Dimethyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat Cyclopentyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat Dimethyl 2,6-diamino-4-(benzo[b]thiophen-3-yl)-1,4-dihydropyridin-3,5-dicarboxylat Cyclopropylmethyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat 4,4-Difluorcyclohexyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat Methyl 2-amino-5-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat 4-Fluorbenzyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-2-amino-4-(5-fluorthieno[2,3-b]pyridin-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat Methyl 2-acetamido-5-acetyl-4-(5-fluorthieno[2,3-b]pyridin-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat Cyclopentylmethyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat 3-(Cyclopropylmethyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat Dimethyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-cyclopropyl-1,4-dihydropyridin-3,5-dicarboxylat 3-(Cyclopropylmethyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-cyclopropyl-1,4-dihydropyridin-3,5-dicarboxylat Dimethyl 2,6-diamino-4-(7-cyanobenzo[b]thiophen-3-yl)-1,4-dihydropyridin-3,5-dicarboxylat 5-(Cyclopropylmethyl) 3-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-cyclopropyl-1,4-dihydropyridin-3,5-dicarboxylat 3-(4-Fluorbenzyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat 3-(4-Fluorbenzyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-cyclopropyl-1,4-dihydropyridin-3,5-dicarboxylat Cyclopropylmethyl 2-amino-5-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat 4-Fluorbenzyl 2-amino-5-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat Cyclopropylmethyl 6-amino-5-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-1,4-dihydropyridin-3-carboxylat 3-Cyclopentyl 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat Cyclopropylmethyl 5-acetyl-2-amino-4-(6-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat Cyclohexyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat Cyclohexylmethyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat Cyclopropylmethyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-5-(cyclopropancarbonyl)-6-methyl-1,4-dihydropyridin-3-carboxylat 3-Fluorbenzyl 5-acetyl-2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat 3-(Cyclobutylmethyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat 3-((3,3-Difluorcyclobutyl)methyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat Cyclopropylmethyl 2-amino-5-carbamoyl-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat 3-((2,2-Difluorcyclopropyl)methyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat 5-Cyclopropyl 3-(cyclopropylmethyl) 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat 3-((2,2-Difluorcyclopropyl)methyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-cyclopropyl-1,4-dihydropyridin-3,5-dicarboxylat 3-(Cyclopropylmethyl) 5-methyl 2-amino-4-(7-cyano-5-fluorbenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat 3-(Cyclopropylmethyl) 5-methyl 2-amino-4-(7-cyano-4-fluorbenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat 3-Isopropyl 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat 3-((2,2-Difluor-3,3-dimethylcyclopropyl)methyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat 5-Methyl 3-neopentyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat 3-(Cyclopropylmethyl) 5-methyl 2-amino-4-(7-cyanothieno[3,2-b]pyridin-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat 5-Methyl 3-neopentyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-cyclopropyl-1,4-dihydropyridin-3,5-dicarboxylat Bis(cyclopropylmethyl) 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat 3-(Cyclopropylmethyl) 5-(prop-2-yn-1-yl) 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat 3-(Cyclopropylmethyl) 5-methyl 2-amino-4-(5,7-dicyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat 5-(But-2-yn-1-yl) 3-(cyclopropylmethyl) 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat 5-Methyl 3-(2,2,2-trifluorethyl) 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat 3-(Cyclopropylmethyl) 5-(2,2,2-trifluorethyl) 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat 3-(Cyclopropylmethyl) 5-methyl 2-amino-4-(6-chlor-7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat 3-(2-Fluor-2-methylpropyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat 3-(Cyclopropylmethyl) 5-methyl 2-amino-4-(7-cyano-6-(trifluormethyl)benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat 5-Methyl 3-prop-2-yn-1-yl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat 3-(Cyclopropylmethyl) 5-methyl 2-amino-4-(7-cyanobenzo[b]thiophen-3-yl)-6-(trifluormethyl)-1,4-dihydropyridin-3,5-dicarboxylat 4-(Benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3,5-dicarbonitril 4-(6-Hydroxybenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3,5-dicarbonitril 4-(6-Methoxybenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3,5-dicarbonitril 2,6-Dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3,5-dicarbonitril 4-(Benzo[b]thiophen-3-yl)-2-methyl-6-phenyl-1,4-dihydropyridin-3,5-dicarbonitril 4-(Benzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridin-3,5-dicarbonitril 5-Acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carbonitril Ethyl 4-(benzo[b]thiophen-3-yl)-5-cyano-2,6-dimethyl-1,4-dihydropyridin -3-carboxylat Ethyl 4-(benzo[b]thiophen-3-yl)-5-cyano-2-methyl-6-phenyl-1,4-dihydropyridin-3-carboxylat Ethyl 4-(benzo[b]thiophen-3-yl)-5-cyano-2-cyclopropyl-6-methyl-1,4-dihydropyridin-3-carboxylat Methyl 4-(benzo[b]thiophen-3-yl)-5-cyano-2-cyclopropyl-6-methyl-1,4-dihydropyridin-3-carboxylat 1,1'-(4-(Benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3,5-diyl)diethanon 1,1 '-(4-(6-Hydroxybenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3,5-diyl)bis(ethan-1-on) 1,1 '-(4-(6-Methoxybenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3,5-diyl)diethanon 1,1'-(4-(Benzo[b]thiophen-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridin-3,5-diyl)diethanon 1,1'-(4-(5-Chlorbenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3,5-diyl)diethanon 1,1'-(4-(5-Brombenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3,5-diyl)diethanon 1,1'-(4-(5-Fluorbenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3,5-diyl)bis(ethan-1-on) 1,1'-(2,6-Dimethyl-4-(5-morpholinobenzo[b]thiophen-3-yl)-1,4-dihydropyridin-3,5-diyl)bis(ethan-1-on) 1,1'-(2,6-Dimethyl-4-(5-(4-methylpiperazin-1-yl)benzo[b]thiophen-3-yl)-1,4-dihydropyridin-3,5-diyl)bis(ethan-1-on) 1,1'-(4-(5-(Benzylamino)benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3,5-diyl)bis(ethan-1-on) 3-(3,5-Diacetyl-2,6-dimethyl-1,4-dihydropyridin-4-yl)benzo[b]thiophen-5-carbonitril 3-(3,5-Diacetyl-2,6-dimethyl-1,4-dihydropyridin-4-yl)benzo[b]thiophen-5-carbonsäure N-Cyclopropyl-3-(3,5-diacetyl-2,6-dimethyl-1,4-dihydropyridin-4-yl)benzo[b]thiophen-5-carboxamid N-(Cyclopropylmethyl)-3-(3,5-diacetyl-2,6-dimethyl-1,4-dihydropyridin-4-yl)benzo[b]thiophen-5-carboxamid 1,1'-(2,6-Dimethyl-4-(5-(4-methylpiperazin-1-carbonyl)benzo[b]thiophen-3-yl)-1,4-dihydropyridin-3,5-diyl)bis(ethan-1-on) 1,1'-(2,6-Dimethyl-4-(5-(morpholin-4-carbonyl)benzo[b]thiophen-3-yl)-1,4-dihydropyridin-3,5-diyl)bis(ethan-1-on) 1,1'-(2,6-Dimethyl-4-(thieno[3,2-c]pyridin-3-yl)-1,4-dihydropyridin-3,5-diyl)bis(ethan-1-on) 1,1'-(2,6-Dimethyl-4-(thieno[2,3-c]pyridin-3-yl)-1,4-dihydropyridin-3,5-diyl)bis(ethan-1-on) 1,1'-(2,6-Dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3,5-diyl)bis(ethan-1-on) Dimethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-2,3-dicarboxylat Dimethyl 5-acetyl-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-2,3-dicarboxylat Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridin-3-carboxylat Dimethyl 4-(benzo[b]thiophen-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridin-3,5-dicarboxylat Ethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(5-chlorbenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat 1,1'-(4-(Benzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydro-pyridin-3,5-diyl)diethanon 1-(4-(Benzo[b]thiophen-3-yl)-2,6-dimethyl-5-(methylsulfonyl)-1,4-dihydropyridin-3-yl)ethanon Methyl 5-acetyl-4-(5-fluorbenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(5-brombenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(5-cyanobenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat 3-(3-Acetyl-5-(methoxycarbonyl)-2,6-dimethyl-1,4-dihydropyridin-4-yl)benzo[b]thiophen-5-carbonsäure Methyl 5-acetyl-4-(5-(cyclopropylcarbamoyl)benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(5-((cyclopropylmethyl)carbamoyl)benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-2,6-dimethyl-4-(5-(4-methylpiperazin-1-carbonyl)benzo[b]thiophen-3-yl)-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-2,6-dimethyl-4-(5-(morpholin-4-carbonyl)benzo[b]thiophen-3-yl)-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-2,6-dimethyl-4-(thieno[3,2-c]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-c]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-2-cyclopropyl-4-(5-fluorbenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat Dimethyl 2,6-dicyclopropyl-4-(5-fluorbenzo[b]thiophen-3-yl)-1,4-dihydropyridin-3,5-dicarboxylat Methyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat Dimethyl 2,6-dicyclopropyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3,5-dicarboxylat Methyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(5-chlorbenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridin-3-carboxylat Dimethyl 4-(5-chlorbenzo[b]thiophen-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridin-3,5-dicarboxylat Methyl 5-acetyl-4-(7-brombenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-2-cyclopropyl-4-(7-(cyclopropylcarbamoyl)benzo[b] thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridin-3-carboxylat Dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridin-3,5-dicarboxylat Methyl 5-acetyl-2-cyclopropyl-4-(7-(methoxycarbonyl) benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat Dimethyl 2,6-dicyclopropyl-4-(7-(methoxycarbonyl)benzo[b]thiophen-3-yl)-1,4-dihydropyridin-3,5-dicarboxylat 3-(3-Acetyl-6-cyclopropyl-5-(methoxycarbonyl)-2-methyl-1,4-dihydropyridin-4-yl)benzo[b]thiophen-7-carbonsäure Benzyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat Pyridin-4-ylmethyl 5-acetyl-4-(benzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridin-3-carboxylat Bis(pyridin-4-ylmethyl) 2,6-dicyclopropyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3,5-dicarboxylat Pyridin-4-ylmethyl 5-acetyl-4-(7-brombenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Pyridin-4-ylmethyl 5-acetyl-4-(5-chlorbenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridin-3-carboxylat Pyridin-4-ylmethyl 5-acetyl-4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridin-3-carboxylat Pyridin-4-ylmethyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat Benzyl 5-acetyl-2-cyclopropyl-4-(7-(methoxycarbonyl)benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat Dibenzyl 2,6-dicyclopropyl-4-(7-(methoxycarbonyl)benzo[b]thiophen-3-yl)-1,4-dihydropyridin-3,5-dicarboxylat Benzyl 5-acetyl-4-(7-(methoxycarbonyl)benzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat Methyl 3-(3,5-diacetyl-2,6-dimethyl-1,4-dihydropyridin-4-yl)benzo[b]thiophen-7-carboxylat 3-(3,5-Diacetyl-2,6-dimethyl-1,4-dihydropyridin-4-yl)benzo[b]thiophen-7-carbonitril Pyridin-4-ylmethyl 5-acetyl-4-(7-brombenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridin-3-carboxylat 4-Fluorbenzyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat Bis(4-fluorbenzyl) 2,6-dicyclopropyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3,5-dicarboxylat 4-Cyanobenzyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(7-chlorbenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-2-cyclopropyl-6-methyl-4-(7-(trifluormethyl)benzo[b]thiophen-3-yl)-1,4-dihydropyridin-3-carboxylat 3-Chlorbenzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat 2-Phenylpropan-2-yl 5-acetyl-4-(7-brombenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridin-3-carboxylat Cyclohexyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat 2-Phenylpropan-2-yl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat 1-(4-(7-Brombenzo[b]thiophen-3-yl)-5-(cyclopropancarbonyl)-6-cyclopropyl-2-methyl-1,4-dihydropyridin-3-yl)ethan-1 -on 3-Chlorbenzyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat 2-(4-Fluorphenyl)propan-2-yl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat 2-(4-Fluorphenyl)propan-2-yl 5-acetyl-4-(7-brombenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridin-3-carboxylat 2-(4-Fluorphenyl)propan-2-yl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-2-cyclopropyl-4-(7-fluorbenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat Cyclopropylmethyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat Cyclopentyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat Cyclopropylmethyl 5-acetyl-4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(7-brombenzo[b]thiophen-3-yl)-2-cyclobutyl-6-methyl-1,4-dihydropyridin-3-carboxylat Dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat Methyl 5-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(7-brombenzo[b]thiophen-3-yl)-2-cyclopentyl-6-methyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-4-(7-brombenzo[b]thiophen-3-yl)-2-cyclohexyl-6-methyl-1,4-dihydropyridin-3-carboxylat Methyl 5-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridin-3-carboxylat 3-((4-Methylpiperazin-1-yl)methyl)benzyl 5-acetyl-4-(7-brombenzo[b]thiophen-3-yl)-2,6-dimethyl-1,4-dihydropyridin-3-carboxylat 3-(Cyclopropylmethyl) 5-methyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat 3-(Cyclopropylmethyl) 5-methyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridin-3,5-dicarboxylat Cyclopropylmethyl 5-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridin-3-carboxylat Dimethyl 2-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-6-cyclopropyl-1,4-dihydropyridin-3,5-dicarboxylat Dimethyl 2-cyano-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat Methyl 5-acetyl-2-((2-aminoethoxy)methyl)-4-(benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-2-((2-aminoethoxy)methyl)-4-(7-brombenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-2-((2-aminoethoxy)methyl)-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat Dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-formyl-1,4-dihydropyridin-3,5-dicarboxylat Dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-(hydroxymethyl)-1,4-dihydropyridin-3,5-dicarboxylat Dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-formyl-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat Dimethyl 4-(7-cyanobenzo[b]thiophen-3-yl)-2-(hydroxymethyl)-6-methyl-1,4-dihydropyridin-3,5-dicarboxylat Dimethyl 2-cyano-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3,5-dicarboxylat Cyclopropylmethyl 5-acetyl-4-(6-cyanobenzo[b]thiophen-3-yl)-2-cyclopropyl-6-methyl-1,4-dihydropyridin-3-carboxylat Cyclopropylmethyl 2,5-diacetyl-4-(7-cyanobenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-2-amino-4-(benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat hydrochlorid Methyl 5-acetyl-2-amino-4-(benzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat trifluormethansulfonat 4-(Pyridin-4-yl)benzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4 dihydropyridin-3-carboxylat 4-(Pyridin-3-yl)benzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat 3-(Pyridin-4-yl)benzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat 3-(Pyridin-3-yl)benzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat 2-(Pyridin-4-yl)benzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat 2-(Pyridin-3-yl)benzyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat [3,4'-Bipyridin]-5-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat [3,3'-Bipyridin]-5-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat [2,4'-Bipyridin]-5-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat [2,3'-Bipyridin]-5-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat [2,3'-Bipyridin]-4-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat [2,4'-Bipyridin]-4-ylmethyl 5-acetyl-2,6-dimethyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-2-cyclopropyl-6-methyl-4-(7-(pyridin-4-yl)benzo[b]thiophen-3-yl)-1,4-dihydropyridin-3-carboxylat Methyl 5-acetyl-2-cyclopropyl-4-(7-cyclopropylbenzo[b]thiophen-3-yl)-6-methyl-1,4-dihydropyridin-3-carboxylat 3-(Pyridin-4-yl)benzyl 5-acetyl-2-cyclopropyl-6-methyl-4-(thieno[2,3-b]pyridin-3-yl)-1,4-dihydropyridin-3-carboxylat 1,1'-(2,6-Dimethyl-4-(5-(pyridin-3-yl)benzo[b]thiophen-3-yl)-1,4-dihydropyridin-3,5-diyl)diethanon Methyl 5-acetyl-2-cyclopropyl-6-methyl-4-(7-phenylbenzo[b]thiophen-3-yl)-1,4-dihydropyridin-3-carboxylat. Verbindung wie in irgendeinem der Ansprüche 1 bis 13 definiert zur Verwendung bei der Behandlung einer Erkrankung oder eines pathologischen Zustands, empfänglich für eine Verbesserung durch Antagonismus des Androgenrezeptors und/oder Glucocorticoidrezeptors, ausgewählt aus Prostatakrebs, kastrationsresistentem Prostatakrebs, Bauchspeicheldrüsenkrebs, Blasenkrebs, Nierenkrebs, Magenkrebs, Lungenkrebs, Brustkrebs, Darmkrebs, Kolorektalkrebs, Eierstockkrebs und anderen festen Tumoren, Melanom, Metastasierungskrebs, benigner Prostatahyperplasie, polyzystischem Ovarialsyndrom (PCOS), Haarverlust, Hirsutismus, Akne, Hypogonadismus, Muskelschwunderkrankungen und Kachexie und Cushing-Syndrom, durch antipsychotische Arzneimittel induzierter Gewichtszunahme, Fettleibigkeit, posttraumatischer Belastungsstörung und Alkoholismus. Eine pharmazeutische Zusammensetzung, umfassend eine Verbindung wie in irgendeinem der Ansprüche 1 bis 13 definiert und ein pharmazeutisch annehmbares Verdünnungsmittel oder einen pharmazeutisch annehmbaren Träger. Eine pharmazeutische Zusammensetzung gemäß Anspruch 15, ferner umfassend eine therapeutisch wirksame Menge eines therapeutischen Mittels, ausgewählt aus Mitteln zur Behandlung von Prostatakrebs, kastrationsresistentem Prostatakrebs, Bauchspeicheldrüsenkrebs, Blasenkrebs, Nierenkrebs, Magenkrebs, Lungenkrebs, Brustkrebs, Darmkrebs, Kolorektalkrebs, Eierstockkrebs und anderen festen Tumoren, Melanom, Metastasierungskrebs, benigner Prostatahyperplasie, polyzystischem Eierstocksyndrom (PCOS), Haarverlust, Hirsutismus, Akne, Hypogonadismus, Muskelschwunderkrankungen und Kachexie und Cushing-Syndrom, durch antipsychotische Arzneimittel induzierter Gewichtszunahme, Fettleibigkeit, posttraumatischer Belastungsstörung und Alkoholismus. Ein Kombinationsprodukt, umfassend eine Verbindung gemäß irgendeinem der Ansprüche 1 bis 13 und ein therapeutisches Mittel, das für die Behandlung von Prostatakrebs, kastrationsresistentem Prostatakrebs, Bauchspeicheldrüsenkrebs, Blasenkrebs, Nierenkrebs, Magenkrebs, Lungenkrebs, Brustkrebs, Darmkrebs, Kolorektalkrebs, Eierstockkrebs und anderen festen Tumoren, Melanom, Metastasierungskrebs, benigner Prostatahyperplasie, polyzystischem Ovarialsyndrom (PCOS), Haarverlust, Hirsutismus, Akne, Hypogonadismus, Muskelschwunderkrankungen und Kachexie und Cushing-Syndrom, durch antipsychotische Arzneimittel induzierter Gewichtszunahme, Fettleibigkeit, posttraumatischer Belastungsstörung und Alkoholismus verwendet wird, insbesondere ein therapeutisches Mittel ausgewählt aus Gonadotropin-Releasing Hormon (GnRH)-Rezeptoragonisten oder -Antagonisten, Androgenrezeptorantagonisten, CYP17-Inhibitor, VEGF-Inhibitor, EGFR-Inhibitor, P13K-Inhibitor, AKT-Inhibitor, mTOR-Inhibitor, c-Met-Inhibitor, Src-Inhibitor, PARP-Inhibitor, Angiopoietin, ALK-Inhibitor, ROS-1-Inhibitor, Anti-(IGF)-Antikörpern, Taxan-Antineoplastikum, Topoisomerase II-Inhibitor, Anti-Tumor-Antibiotikum, HSP90-Inhibitor, Aurora-Kinase-Inhibitor, einem auf PSA gerichteten Impfstoff, GR-Antagonisten, 11-beta-HSD-Inhibitoren, einem oder mehreren immuntherapeutischen Mitteln, ausgewählt aus der Gruppe bestehend aus Anti-CTLA4- Antikörpern, Anti-PD1-Antikörpern und Anti-PDL1-Antikörpern, insbesondere einem Antikörper ausgewählt aus der Gruppe bestehend aus Ipilimumab, Tremelimumab, Nivolumab, Pembrolizumab, CT-011, AMP-224, MPDL3280A, MEDI4736 und MDX-1105. Composé de formule (I) : où : - R1 est un groupe choisi parmi : a) -COR5, b) -COOR5, c) -CN, d) -C(O)NH2 - R5 est un groupe choisi parmi : a) alkyle en C1-C6 linéaire ou ramifié éventuellement substitué par 1, 2 ou 3 substituants choisis parmi -N(R6)R7 et -OR6, un atome d'halogène, cycloalkyle en C3-C6 et alcynyle, b) cycloalkyle en C3-C6, - R2 est un groupe choisi parmi : a) -COOR8, b) -COR8, c) -C(O)N(R8)R9, d) -CN, e) -S(O)nR8, où n est un nombre entier de 1 à 2, - R8 et R9 sont indépendamment choisis parmi : a) alkyle en C1-C6 linéaire ou ramifié éventuellement substitué par 1, 2 ou 3 substituants choisis parmi A1 ou B2, b) groupe A1, c) atome d'hydrogène,
ou - R8 et R9 ensemble avec l'atome d'azote auquel ils sont attachés forment un hétérocycle à 5-6 éléments qui comprend éventuellement 1 hétéroatome choisi parmi 0 et N, et ledit hétérocycle étant éventuellement substitué par 1 ou 2 groupes indépendamment choisis parmi un alkyle en C1-C4 linéaire ou ramifié - R3 est un groupe choisi parmi : a) alkyle en C1-C6 linéaire ou ramifié éventuellement substitué par 1, 2 ou 3 substituants choisis parmi un atome d'halogène, -N(R6)R7, et -OR6, b) cycloalkyle en C3-C6 éventuellement substitué par 1, 2 ou 3 atomes d'halogène, c) atome d'hydrogène, d) -NH2, e) -CN, - R4 est un groupe choisi parmi : a) groupe A1, b) alkyle en C1-C6 linéaire ou ramifié éventuellement substitué par 1, 2 ou 3 substituants choisis parmi A1 ou B2, c) -N(R6)R7, d) -CN, e) -CO-H ; f) -CO-Me et g) CO-OMe h) atome d'hydrogène, - X1, X2, X3, X4, et X5 sont indépendamment choisis parmi C-B1, N et C-H, - A1 est choisi parmi : a) cycloalkyle en C3-C6 lequel noyau est éventuellement substitué par 1, 2, 3 ou 4 substituants choisis parmi =0 et B3; b) un noyau hétérocyclyle saturé à de 3 à 6 éléments comprenant 1, 2 ou 3 hétéroatomes choisis parmi 0, S et N, et lequel noyau est éventuellement substitué par 1, 2 ou 3 substituants choisis parmi =0 et B3 ; c) groupe phényle ou hétéroaryle à de 5 à 6 éléments, l'un ou l'autre est éventuellement substitué par 1, 2 ou 3 substituants choisis parmi B1 ; - chaque B1 est indépendamment choisi parmi un atome d'halogène, groupe -CF3, hétéroaryle à de 5 à 6 éléments, alkyle en C1-C6 linéaire ou ramifié, -CN, -N(R6)R7, -OR6, -C(=O)R6, -C(=O)OR6, -C(=O)N(R6)R7, -OC(=O)-R6, -N(R6)C(=O)R7, -NR7SO2R6, -SO2N(R6)R7, -SR6, -S(O)R6 et -S(O)2R6, - chaque B2 est indépendamment choisi parmi un atome d'halogène, -CN, -N(R6)R7, -OR6, -C(=O)R6, -C(=O)OR6, -C(=O)N(R6)R7, -OC(=O)-R6,-N(R6)C(=O)R7, -NR7SO2R6, -SO2N(R6)R7, -SR6, -S(O)R6, -S(O)2R6, et groupe alcynyle, - chaque B3 est indépendamment choisi parmi un atome d'halogène, groupe alkyle en C1-C6 linéaire ou ramifié, -CN, -N(R6)R7, -OR6, -C(=O)R6, -C(=O)OR6, -C(=O)N(R6)R7, -OC(=O)-R6, -N(R6)C(=O)R7, -NR7SO2R6, -SO2N(R6)R7, -SR6, -S(O)R6, -S(O)2R6, chaque R6 et R7 représente indépendamment : - un atome d'hydrogène, - alkyle en C1-C12 linéaire ou ramifié, cycloalkyle en C3-C6 et hétérocycloalkyle en C4-C6, lesquels sont éventuellement substitués par 1, 2 ou 3 substituants choisis parmi =O (oxo), un atome d'halogène, hydroxy, phényle, cycloalkyle en C3-C6, alcoxy en C1-C6 linéaire ou ramifié, amino, alkylamino, dialkylamino, alkylcarbonyle en C1-C6 linéaire ou ramifié, - phényle ou hétéroaryle à de 5 à 6 éléments, lesquels sont éventuellement substitués par 1, 2 ou 3 substituants choisis parmi un atome d'halogène, groupe cyano, alkyle en C1-C6 linéaire ou ramifié, haloalkyle en C1-C6 linéaire ou ramifié, hydroxy, alcoxy en C1-C6 linéaire ou ramifié, amino, alkylamino, dialkylamino ; - R6 et R7 forment avec l'atome d'azote auquel ils sont fixés, un noyau à de 3 à 8 éléments qui contient éventuellement un autre hétéroatome choisi parmi O, N et S, et lequel noyau est éventuellement substitué par 1, 2 ou 3 substituants choisis parmi =0 (oxo), alkyle en C1-C6 linéaire ou ramifié, haloalkyle en C1-C6 linéaire ou ramifié, alkylcarbonyle en C1-C6 linéaire ou ramifié ; - à condition que lorsque R1 est -COOR5 et R2 est -COOR8 alors R4 n'est pas un groupe méthyle, et sels pharmaceutiquement acceptables de celui-ci. Composé selon la revendication 1, où X1, X2, X3 et X5 représentent C-H ou C-B1, où B1 représente un atome d'halogène. Composé selon la revendication 2, où X4 est un groupe choisi parmi C-B1 et N. Composé selon la revendication 3, où B1 est choisi parmi un groupe -CN et un atome d'halogène. Composé selon l'une quelconque des revendications 2 à 4, où R1 est un groupe choisi parmi -COR5, -COOR5 et -CN. Composé selon la revendication 5, où R5 est choisi parmi : - un groupe alkyle en C1-C4 où le méthyle terminal est non substitué ou substitué par trois atomes de fluor (-CF3) - un groupe alkyle en C1-C3 éventuellement substitué à une position quelconque par un groupe alcynyle, et - un groupe cycloalkyle en C3-C5. Composé selon l'une quelconque des revendications 2 à 6, où R2 représente -COOR8. Composé selon la revendication 7, où R8 représente indépendamment : - alkyle en C1-C6 linéaire ou ramifié éventuellement substitué par 1, 2 ou 3 substituants choisis parmi des atomes de fluor et cycloalkyle en C3-C5 éventuellement substitué par 1, 2 ou 3 atomes de fluor, ou - groupe A1, qui représente un cycloalkyle en C3-C6 qui est éventuellement substitué par 1, 2 ou 3 substituants choisis dans le groupe consistant en atomes de fluor et groupes alkyle en C1-C3. Composé selon l'une quelconque des revendications 2 à 8, où R3 est un groupe choisi parmi un alkyle en C1-C4 linéaire ou ramifié et cycloalkyle en C3-C4, lesdits groupes étant éventuellement substitués par 1, 2 ou 3 atomes de fluor. Composé selon l'une quelconque des revendications 2 à 9, où R4 est un groupe choisi parmi : - -N(R6)R7, où R6 et R7 sont indépendamment choisis parmi un atome d'hydrogène et un alkyle en C1-C3 linéaire ou ramifié, - un groupe A1, qui représente un cycloalkyle en C3-C6, - alkyle en C1-C3 linéaire ou ramifié, éventuellement substitué par 1, 2 ou 3 substituants choisis parmi 1, 2 ou 3 atomes de fluor ou 1 groupe hydroxyle. Composé selon la revendication 1, où X1, X2, X3 et X5 représentent -CH, X4 représente C-B1, où B1 représente un groupe -CN ou atome de brome, R1 est un groupe choisi parmi -C(O)CH3, -C(O)OCH3, -C(O)OCH2-alcynyle et CN, R2 est un groupe choisi parmi un -C(O)O-groupe alkyle en C1-C5 linéaire ou ramifié éventuellement substitué par 1, 2 ou 3 atomes de fluor et C(O)OCH2-cyclopropyle éventuellement substitué par 1, 2 ou 3 atomes de fluor, R3 est un groupe choisi parmi un groupe alkyle en C1-C3 linéaire ou ramifié et cycloalkyle en C3-C4 et R4 est un groupe choisi parmi un alkyle en C1-C3 linéaire ou ramifié, cycloalkyle en C3-C4 et -NH2. Composé selon la revendication 1, où X1, X2, X3 et X5 représentent un -CH, X4 représente C-B1, où B1 représente un groupe -CN, R1 est un groupe choisi parmi -C(O)CH3, -C(O)OCH3, R2 est un groupe choisi parmi -C(O)OCH2-cyclopropyle éventuellement substitué par 1, 2 ou 3 atomes de fluor, et -C(O)OCH2-CF3, R3 est un groupe choisi parmi méthyle et cyclopropyle et R4 est un groupe choisi parmi cyclopropyle et -NH2. Composé selon la revendication 1, qui est choisi dans le groupe consistant en : 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydro-pyridine-3-carboxylate de méthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydro-pyridine-3-carboxylate de méthyle (Enantiomère 1) 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydro-pyridine-3-carboxylate de méthyle (Enantiomère 2) 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxamide 1-(4-(benzo[b]thiophén-3-yl)-5-benzoyl-2,6-diméthyl-1,4-dihydropyridin-3-yl)éthan-1-one 5-acétyl-4-(benzo[b]thiophén-3-yl)-6-méthyl-2-phényl-1,4-dihydropyridine-3-carboxylate de méthyle 1-(4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-5-nicotinoyl-1,4-dihydro pyridin-3-yl)éthan-1-one 5-acétyl-4-(benzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydro-[2,3'-bipyridine]-3-carboxylate de méthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de 2,2,2-trifluoroéthyle acide 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydro-pyridine-3-carboxylique 1-(4-(Benzo[b]thiophén-3-yl)-2,6-diméthyl-5-(4-méthylpipérazine-1-carbonyl)-1,4-dihydropyridin-3-yl)éthan-1-one 5-Acétyl-4-(benzo[b]thiophén-3-yl)-N,N-diéthyl-2,6-diméthyl-1,4-dihydropyridine-3-carboxamide 1-(4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-5-(morpholine-4-carbonyl)-1,4-dihydropyridin-3-yl)éthan-1-one 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de 2-méthoxyéthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de 3-acétamidopropyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydro-pyridine-3-carboxylate de benzyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de 2-morpholinoéthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de 2-(diméthylamino)éthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de 2-acétamidoéthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2-(méthoxyméthyl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2-((diméthylamino)méthyl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de pyridine-4-ylméthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de 4-méthoxybenzyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de pyridine-2-ylméthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-6-méthyl-2-(morpholino-méthyl)-1,4-dihydropyridine-3-carboxylate de méthyle 4-(benzo[b]thiophén-3-yl)-2,6-bis(morpholinométhyl)-1,4-dihydropyridine-3,5-dicarboxylate de diméthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de 2-hydroxyéthyle 1-(4-(benzo[b]thiophén-3-yl)-5-(cyclopropanecarbonyl)-6-cyclopropyl-2-méthyl-1,4-dihydropyridin-3-yl)éthan-1-one 5-acétyl-4-(benzo[b]thiophén-3-yl)-2-(1-(tert-butoxycarbonyl) azétidin-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-4-(benzo[b] thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de (1-(tert-butoxycarbonyl)pipéridin-4-yl)méthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de cyclohexylméthyle 4-(benzo[b]thiophén-3-yl)-5-(cyclopropanecarbonyl)-2,6-dicyclopropyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-N-phényl-1,4-dihydropyridine-3-carboxamide 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de tétrahydro-2H-pyran-4-yle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de (tétrahydro-2H-pyran-4-yl)méthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de cyclohexyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2-(2-méthoxy-2-oxoéthyl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-2,6-diméthyl-4-(2-méthylbenzo[b]thiophén-3-yl)-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de cyclopropylméthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2-(2-méthoxyéthyl)-6-méthyl-1,4-dihydropyridine-3-carboxylatedeméthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2-((benzyloxy)méthyl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-6-méthyl-2-(phénoxyméthyl)-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de phénéthyle 4-(benzo[b]thiophén-3-yl)-5-(cyclopropanecarbonyl)-6-cyclopropyl-2-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-6-méthyl-2-(trifluorométhyl)-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-6-benzyl-2-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-5-(2-phénylacétyl)-1,4-dihydropyridine-3-carboxylate de méthyle 4-(benzo[b]thiophén-3-yl)-5-(2-méthoxyacétyl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-6-(méthoxyméthyl)-2-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2-(fluorométhyl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-4-(5-fluorobenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de cyclopropylméthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de 1-(tert-butoxycarbonyl)pipéridin-4-yle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de cyclopentylméthyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de cyclopropylméthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de 1-méthylpipéridin-4-yle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de cyclopentyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de 4,4-diméthylcyclohexyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de cyclobutyle 5-acétyl-4-(5-fluorobenzo[b]thiophén-3-yl)-6-méthyl-2-(trifluorométhyl)-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-2,6-diméthyl-4-(thiéno[3,2-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2-méthyl-6-(trifluorométhyl)-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-4-(5-chlorobenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de cyclopropylméthyle 5-acétyl-4-(5-chlorobenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de cyclohexylméthyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de cyclohexylméthyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de cyclopentylméthyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de cyclohexyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de cyclopentyle 5-acétyl-4-(5-chlorobenzo[b]thiophén-3-yl)-6-méthyl-2-(trifluorométhyl)-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de benzyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de (tétrahydro-2H-pyran-4-yl)méthyle 5-acétyl-4-(5-fluorobenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de benzyle 5-acétyl-4-(5-chlorobenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de benzyle 5-acétyl-4-(5-chlorobenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de (tétrahydro-2H-pyran-4-yl)méthyle 5-acétyl-4-(5-fluorobenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de (tétrahydro-2H-pyran-4-yl)méthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-6-méthyl-2-(trifluorométhyl)-1,4-dihydropyridine-3-carboxylate de benzyle 5-acétyl-4-(5-fluorobenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de pyridine-4-ylméthyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de 4-fluorobenzyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de pyridin-3-ylméthyle 1,1'-(4-(benzo[b]thiophén-3-yl)-2-benzyl-6-méthyl-1,4-dihydropyridine-3,5-diyl)bis(éthan-1-one) 1-(5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridin-3-yl)-2-phényléthan-1-one 5-acétyl-6-méthyl-4-(thiéno[2,3-b]pyridin-3-yl)-2-(trifluorométhyl)-1,4-dihydropyridine-3-carboxylate de méthyle 1-(2-méthyl-5-(pipéridine-1-carbonyl)-4-(thiéno[2,3-b]pyridin-3-yl)-6-(trifluorométhyl)-1,4-dihydropyridin-3-yl)éthan-1-one acide 4-(((5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydro-pyridine-3-carbonyl)oxy)méthyl)benzoïque 5-acétyl-6-méthyl-4-(thiéno[2,3-b]pyridin-3-yl)-2-(trifluorométhyl)-1,4-dihydropyridine-3-carboxylate de benzyle 5-acétyl-4-(5-fluorobenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de pyridin-3-ylméthyle 5-acétyl-4-(7-bromobenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de pyridin-3-ylméthyle 5-acétyl-4-(7-cyanobenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydro-pyridine-3-carboxylate de pyridin-3-ylméthyle 5-acétyl-4-(5-chlorobenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydro-pyridine-3-carboxylate de pyridin-3-ylméthyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de pyridin-3-ylméthyle 5-acétyl-4-(5-chlorobenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydro-pyridine-3-carboxylate de pyridin-4-ylméthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de 4-(cyclopropylcarbamoyl)benzyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de pyridin-4-ylméthyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de 4-bromobenzyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de 3-bromobenzyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de 2-bromobenzyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de (3-fluoropyridin-4-yl)méthyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de pyrimidin-5-ylméthyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de (5-bromopyridin-3-yl)méthyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de 2-phénylpropan-2-yle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de 3-cyanobenzyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de 4-cyanobenzyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de (6-chloropyridin-3-yl)méthyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de 3-morpholinobenzyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de 4,4-diméthylcyclohexyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de (2-chloropyridin-4-yl)méthyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de tétrahydro-2H-pyran-4-yle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de 4,4-difluorocyclohexyle 5-acétyl-N-benzyl-N,2,6-triméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxamide 5-acétyl-4-(7-bromobenzo[b]thiophén-3-yl)-2-cyclopropyl-6-méthyl-1,4-dihydropyridine-3-carboxylate d'oxétan-3-yle 5-acétyl-4-(7-bromobenzo[b]thiophén-3-yl)-2-cyclopropyl-6-méthyl-1,4-dihydropyridine-3-carboxylate d'isopropyle 5-acétyl-4-(7-bromobenzo[b]thiophén-3-yl)-6-méthyl-2-(trifluorométhyl)-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-4-(7-bromobenzo[b]thiophén-3-yl)-2-cyclopropyl-6-méthyl-1,4-dihydropyridine-3-carboxylate de cyclopropylméthyle 5-acétyl-2-cyclopropyl-6-méthyl-4-(7-(2,2,2-trifluoroacétyl) benzo[b]thiophén-3-yl)-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-4-(7-bromobenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de 2-phénylpropan-2-yle 5-acétyl-2-amino-4-(benzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 2-amino-4-(benzo[b]thiophén-3-yl)-5-(cyclopropanecarbonyl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-2-amino-4-(5-fluorobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-2-amino-4-(7-bromobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-2-amino-4-(7-bromobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de cyclopentyle 2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de diméthyle 5-acétyl-2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de cyclopentyle 2,6-diamino-4-(benzo[b]thiophén-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate de diméthyle 5-acétyl-2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de cyclopropylméthyle 5-acétyl-2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de 4,4-difluorocyclohexyle 2-amino-5-cyano-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de 4-fluorobenzyle 5-acétyl-2-amino-4-(5-fluorothiéno[2,3-b]pyridin-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 2-acétamido-5-acétyl-4-(5-fluorothiéno[2,3-b]pyridin-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl- 2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de cyclopentylméthyle 2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de 3-(cyclopropylméthyl) 5-méthyle 2-amino-4-(7 -cyanobenzo[b]thiophén-3-yl)-6-cyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate de diméthyle 2-amino-4-(7 -cyanobenzo[b]thiophén-3-yl)-6-cyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate de 3-(cyclopropylméthyl) 5-méthyle 2,6-diamino-4-(7-cyanobenzo[b]thiophén-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate de diméthyle 2-amino-4-(7 -cyanobenzo[b]thiophén-3-yl)-6-cyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate de 5-(cyclopropylméthyl) 3-méthyle 2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de 3-(4-fluorobenzyl) 5-méthyle 2-amino-4-(7 -cyanobenzo[b]thiophén-3-yl)-6-cyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate de 3-(4-fluorobenzyl) 5-méthyle 2-amino-5-cyano-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de cyclopropylméthyle 2-amino-5-cyano-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de 4-fluorobenzyle 6-amino-5-cyano-4-(7-cyanobenzo[b]thiophén-3-yl)-2-cyclopropyl-1,4-dihydropyridine-3-carboxylate de cyclopropylméthyle 5-méthyl 2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de 3-cyclopentyle 5-acétyl-2-amino-4-(6-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de cyclopropylméthyle 5-acétyl-2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de cyclohexyle 5-acétyl-2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de cyclohexylméthyle 2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-5-(cyclopropanecarbonyl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de cyclopropylméthyle 5-acétyl-2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de 3-fluorobenzyle 2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de 3-(cyclobutylméthyl) 5-méthyle 2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de 3-((3,3-difluorocyclobutyl)méthyl) 5-méthyle 2-amino-5-carbamoyl-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de cyclopropylméthyle 2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de 3-((2,2-difluorocyclopropyl)méthyl) 5-méthyle 2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de 5-cyclopropyl 3-(cyclopropylméthyle) 2-amino-4-(7 -cyanobenzo[b]thiophén-3-yl)-6-cyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate de 3-((2,2-difluorocyclopropyl)méthyl) 5-méthyle 2-amino-4-(7-cyano-5-fluorobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de 3-(cyclopropylméthyl) 5-méthyle 2-amino-4-(7-cyano-4-fluorobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de 3-(cyclopropylméthyl) 5-méthyle 2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de 3-isopropyl 5-méthyle 2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de 3-((2,2-difluoro-3,3-diméthylcyclopropyl)méthyl) 5-méthyle 2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de 5-méthyl 3-néopentyle 2-amino-4-(7-cyanothiéno[3,2-b]pyridin-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de 3-(cyclopropylméthyl) 5-méthyle 2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-cyclopropyl-1,4-dihydro-pyridine-3,5-dicarboxylate de 5-méthyl 3-néopentyle 2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de bis(cyclopropylméthyle) 2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de 3-(cyclopropylméthyl) 5-(prop-2-yn-1-yle) 2-amino-4-(5,7-dicyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de 3-(cyclopropylméthyl) 5-méthyle 2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de 5-(but-2-yn-1-yl) 3-(cyclopropylméthyle) 2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de 5-méthyl 3-(2,2,2-trifluoroéthyle) 2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de 3-(cyclopropylméthyl) 5-(2,2,2-trifluoroéthyle) 2-amino-4-(6-chloro-7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de 3-(cyclopropylméthyl) 5-méthyle 2-amino-4-(7-cyanobenzo[b] thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de 3-(2-fluoro-2-méthylpropyl) 5-méthyle 2-amino-4-(7-cyano-6-(trifluorométhyl)benzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de 3-(cyclopropylméthyl) 5-méthyle 2-amino-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de 5-méthyl 3-prop-2-yn-1-yle 2-amino-4-(7 -cyanobenzo[b]thiophén-3-yl)-6-(trifluorométhyl)-1,4-dihydropyridine-3,5-dicarboxylate de 3-(cyclopropylméthyl) 5-méthyle 4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3,5-dicarbonitrile 4-(6-hydroxybenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3,5-dicarbonitrile 4-(6-méthoxybenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3,5-dicarbonitrile 2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3,5-dicarbonitrile 4-(benzo[b]thiophén-3-yl)-2-méthyl-6-phényl-1,4-dihydropyridine-3,5-dicarbonitrile 4-(benzo[b]thiophén-3-yl)-2-cyclopropyl-6-méthyl-1,4-dihydropyridine-3,5-dicarbonitrile 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carbonitrile 4-(benzo[b]thiophén-3-yl)-5-cyano-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate d'éthyle 4-(benzo[b]thiophén-3-yl)-5-cyano-2-méthyl-6-phényl-1,4-dihydropyridine-3-carboxylate d'éthyle 4-(benzo[b]thiophén-3-yl)-5-cyano-2-cyclopropyl-6-méthyl-1,4-dihydropyridine-3-carboxylate d'éthyle 4-(benzo[b]thiophén-3-yl)-5-cyano-2-cyclopropyl-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 1,1'-(4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3,5-diyl)diéthanone 1,1'-(4-(6-hydroxybenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydro-pyridine-3,5-diyl)bis(éthan-1-one) 1,1'-(4-(6-méthoxybenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydro-pyridine-3,5-diyl)diéthanone 1,1'-(4-(benzo[b]thiophén-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridine-3,5-diyl)diéthanone 1,1'-(4-(5-chlorobenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3,5-diyl)diéthanone 1,1'-(4-(5-bromobenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3,5-diyl)diéthanone 1,1'-(4-(5-fluorobenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3,5-diyl)bis(éthan-1-one) 1,1'-(2,6-diméthyl-4-(5-morpholinobenzo[b]thiophén-3-yl)-1,4-dihydropyridine-3,5-diyl)bis(éthan-1-one) 1,1'-(2,6-diméthyl-4-(5-(4-méthylpipérazin-1-yl)benzo[b]thiophén-3-yl)-1,4-dihydropyridine-3,5-diyl)bis(éthan-1-one) 1,1'-(4-(5-(benzylamino)benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3,5-diyl)bis(éthan-1-one) 3-(3,5-diacétyl-2,6-diméthyl-1,4-dihydropyridin-4-yl)benzo[b]thiophène-5-carbonitrile acide 3-(3,5-diacétyl-2,6-diméthyl-1,4-dihydropyridin-4-yl)benzo[b]thiophène-5-carboxylique N-cyclopropyl-3-(3,5-diacétyl-2,6-diméthyl-1,4-dihydropyridin-4-yl)benzo[b]thiophène-5-carboxamide N-(cyclopropylméthyl)-3-(3,5-diacétyl-2,6-diméthyl-1,4-dihydropyridin-4-yl)benzo[b]thiophène-5-carboxamide 1,1'-(2,6-diméthyl-4-(5-(4-méthylpipérazine-1-carbonyl)benzo[b]thiophén-3-yl)-1,4-dihydropyridine-3,5-diyl)bis(éthan-1-one) 1,r-(2,6-diméthyl-4-(5-(morpholine-4-carbonyl)benzo[b]thiophén-3-yl)-1,4-dihydropyridine-3,5-diyl)bis(éthan-1-one) 1,1'-(2,6-diméthyl-4-(thiéno[3,2-c]pyridin-3-yl)-1,4-dihydropyridine-3,5-diyl)bis(éthan-1-one) 1,1'-(2,6-diméthyl-4-(thiéno[2,3-c]pyridin-3-yl)-1,4-dihydropyridine-3,5-diyl)bis(éthan-1-one) 1,1'-(2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3,5-diyl)bis(éthan-1-one) 5-acétyl-4-(benzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-2,3-dicarboxylate de diméthyle 5-acétyl-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-2,3-dicarboxylate de diméthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2-cyclopropyl-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 4-(benzo[b]thiophén-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate de diméthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate d'éthyle 5-acétyl-4-(5-chlorobenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de méthyle 1,1'-(4-(benzo[b]thiophén-3-yl)-2-cyclopropyl-6-méthyl-1,4-dihydro-pyridine-3,5-diyl)diéthanone 1-(4-(benzo[b]thiophén-3-yl)-2,6-diméthyl-5-(méthylsulfonyl)-1,4-dihydro-pyridin-3-yl)éthanone 5-acétyl-4-(5-fluorobenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-4-(5-bromobenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-4-(5-cyanobenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de méthyle acide 3-(3-acétyl-5-(méthoxycarbonyl)-2,6-diméthyl-1,4-dihydropyridin-4-yl)benzo[b]thiophène-5-carboxylique 5-acétyl-4-(5-(cyclopropylcarbamoyl)benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-4-(5-((cyclopropylméthyl)carbamoyl)benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-2,6-diméthyl-4-(5-( 4-méthylpipérazine-1-carbonyl)benzo[b] thiophén-3-yl)-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-2,6-diméthyl-4-(5-(morpholine-4-carbonyl)benzo[b]thiophén-3-yl)-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-2,6-diméthyl-4-(thiéno[3,2-c]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-c]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-2-cyclopropyl-4-(5-fluorobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 2,6-dicyclopropyl-4-(5-fluorobenzo[b]thiophén-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate de diméthyle 5-acétyl-2-cyclopropyl-6-méthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de méthyle 2,6-dicyclopropyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate de diméthyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-4-(5-chlorobenzo[b]thiophén-3-yl)-2-cyclopropyl-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 4-(5-chlorobenzo[b]thiophén-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate de diméthyle 5-acétyl-4-(7-bromobenzo[b]thiophén-3-yl)-2-cyclopropyl-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-2-cyclopropyl-4-(7-(cyclopropylcarbamoyl)benzo[b] thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-4-(7-cyanobenzo[b]thiophén-3-yl)-2-cyclopropyl-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 4-(7-cyanobenzo[b]thiophén-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate de diméthyle 5-acétyl-2-cyclopropyl-4-(7-(méthoxycarbonyl) benzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 2,6-dicyclopropyl-4-(7-(méthoxycarbonyl)benzo[b]thiophén-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate de diméthyle acide 3-(3-acétyl-6-cyclopropyl-5-(méthoxycarbonyl)-2-méthyl-1,4-dihydropyridin-4-yl)benzo[b]thiophène-7-carboxylique 5-acétyl-2-cyclopropyl-6-méthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de benzyle 5-acétyl-4-(benzo[b]thiophén-3-yl)-2-cyclopropyl-6-méthyl-1,4-dihydropyridine-3-carboxylate de pyridin-4-ylméthyle 2,6-dicyclopropyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate de bis(pyridin-4-ylméthyle) 5-acétyl-4-(7-bromobenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de pyridin-4-ylméthyle 5-acétyl-4-(5-chlorobenzo[b]thiophén-3-yl)-2-cyclopropyl-6-méthyl-1,4-dihydropyridine-3-carboxylate de pyridin-4-ylméthyle 5-acétyl-4-(7-cyanobenzo[b]thiophén-3-yl)-2-cyclopropyl-6-méthyl-1,4-dihydropyridine-3-carboxylate de pyridin-4-ylméthyle 5-acétyl-2-cyclopropyl-6-méthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de pyridin-4-ylméthyle 5-acétyl-2-cyclopropyl-4-(7-(méthoxycarbonyl)benzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de benzyle 2,6-dicyclopropyl-4-(7-(méthoxycarbonyl)benzo[b]thiophén-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate de dibenzyle 5-acétyl-4-(7-(méthoxycarbonyl)benzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydropyridine-3-carboxylate de benzyle 3-(3,5-diacétyl-2,6-diméthyl-1,4-dihydropyridin-4-yl)benzo[b]thiophène-7-carboxylate de méthyle 3-(3,5-diacétyl-2,6-diméthyl-1,4-dihydropyridin-4-yl)benzo[b]thiophène-7-carbonitrile 5-acétyl-4-(7-bromobenzo[b]thiophén-3-yl)-2-cyclopropyl-6-méthyl-1,4-dihydropyridine-3-carboxylate de pyridin-4-ylméthyle 5-acétyl-2-cyclopropyl-6-méthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de 4-fluorobenzyle 2,6-dicyclopropyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate de bis(4-fluorobenzyle) 5-acétyl-2-cyclopropyl-6-méthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de 4-cyanobenzyle 5-acétyl-4-(7-chlorobenzo[b]thiophén-3-yl)-2-cyclopropyl-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-2-cyclopropyl-6-méthyl-4-(7-(trifluorométhyl)benzo[b]thiophén-3-yl)-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de 3-chlorobenzyle 5-acétyl-4-(7-bromobenzo[b]thiophén-3-yl)-2-cyclopropyl-6-méthyl-1,4-dihydropyridine-3-carboxylate de 2-phénylpropan-2-yle 5-acétyl-2-cyclopropyl-6-méthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de cyclohexyle 5-acétyl-2-cyclopropyl-6-méthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de 2-phénylpropan-2-yle 1-(4-(7-bromobenzo[b]thiophén-3-yl)-5-(cyclopropanecarbonyl)-6-cyclopropyl-2-méthyl-1,4-dihydropyridin-3-yl)éthan-1-one 5-acétyl-2-cyclopropyl-6-méthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de 3-chlorobenzyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de 2-(4-fluorophényl)propan-2-yle 5-acétyl-4-(7-bromobenzo[b]thiophén-3-yl)-2-cyclopropyl-6-méthyl-1,4-dihydropyridine-3-carboxylate de 2-(4-fluorophényl)propan-2-yle 5-acétyl-2-cyclopropyl-6-méthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de 2-(4-fluorophényl)propan-2-yle 5-acétyl-2-cyclopropyl-4-(7-fluorobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-2-cyclopropyl-6-méthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de cyclopropylméthyle 5-acétyl-2-cyclopropyl-6-méthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de cyclopentyle 5-acétyl-4-(7-cyanobenzo[b]thiophén-3-yl)-2-cyclopropyl-6-méthyl-1,4-dihydropyridine-3-carboxylate de cyclopropylméthyle 5-acétyl-4-(7-bromobenzo[b]thiophén-3-yl)-2-cyclobutyl-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 4-(7-cyanobenzo[b]thiophén-3-yl)-2-cyclopropyl-6-méthyl-1,4-dihydro-pyridine-3,5-dicarboxylate de diméthyle 5-cyano-4-(7-cyanobenzo[b]thiophén-3-yl)-2-cyclopropyl-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-4-(7-bromobenzo[b]thiophén-3-yl)-2-cyclopentyl-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-4-(7-bromobenzo[b]thiophén-3-yl)-2-cyclohexyl-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-cyano-4-(7-cyanobenzo[b]thiophén-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-4-(7-bromobenzo[b]thiophén-3-yl)-2,6-diméthyl-1,4-dihydro-pyridine-3-carboxylate de 3-((4-méthylpipérazin-1-yl)méthyl)benzyle 4-(7-cyanobenzo[b]thiophén-3-yl)-2-cyclopropyl-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de 3-(cyclopropylméthyl) 5-méthyle 4-(7-cyanobenzo[b]thiophén-3-yl)-2,6-dicyclopropyl-1,4-dihydropyridine-3,5-dicarboxylate de 3-(cyclopropylméthyl) 5-méthyle 5-cyano-4-(7-cyanobenzo[b]thiophén-3-yl)-2-cyclopropyl-6-méthyl-1,4-dihydropyridine-3-carboxylate de cyclopropylméthyle 2-cyano-4-(7-cyanobenzo[b]thiophén-3-yl)-6-cyclopropyl-1,4-dihydro-pyridine-3,5-dicarboxylate de diméthyle 2-cyano-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de diméthyle 5-acétyl-2-((2-aminoéthoxy)méthyl)-4-(benzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-2-((2-aminoéthoxy)méthyl)-4-(7-bromobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-2-((2-aminoéthoxy)méthyl)-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 4-(7-cyanobenzo[b]thiophén-3-yl)-2-cyclopropyl-6-formyl-1,4-dihydropyridine-3,5-dicarboxylate de diméthyle 4-(7-cyanobenzo[b]thiophén-3-yl)-2-cyclopropyl-6-(hydroxyméthyl)-1,4-dihydropyridine-3,5-dicarboxylate de diméthyle 4-(7-cyanobenzo[b]thiophén-3-yl)-2-formyl-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de diméthyle 4-(7-cyanobenzo[b]thiophén-3-yl)-2-(hydroxyméthyl)-6-méthyl-1,4-dihydropyridine-3,5-dicarboxylate de diméthyle 2-cyano-6-méthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3,5-dicarboxylate de diméthyle 5-acétyl-4-(6-cyanobenzo[b]thiophén-3-yl)-2-cyclopropyl-6-méthyl-1,4-dihydropyridine-3-carboxylate de cyclopropylméthyle 2,5-diacétyl-4-(7-cyanobenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydro-pyridine-3-carboxylate de cyclopropylméthyle 5-acétyl-2-amino-4-(benzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate hydrochlorure de méthyle 5-acétyl-2-amino-4-(benzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate trifluorométhanesulfonate de méthyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de 4-(pyridin-4-yl)benzyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de 4-(pyridin-3-yl)benzyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-l,4-dihydropyridine-3-carboxylate de 3-(pyridin-4-yl)benzyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de 3-(pyridin-4-yl)benzyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de 2-(pyridin-4-yl)benzyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de 2-(pyridin-3-yl)benzyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de [3,4'-bipyridin]-5-ylméthyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de [3,3'-bipyridin]-5-ylméthyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de [2,4'-bipyridin]-5-ylméthyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de [2,3'-bipyridin]-5-ylméthyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de [2,3'-bipyridin]-4-ylméthyle 5-acétyl-2,6-diméthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de [2,4'-bipyridin]-4-ylméthyle 5-acétyl-2-cyclopropyl-6-méthyl-4-(7-(pyridin-4-yl)benzo[b]thiophén-3-yl)-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-2-cyclopropyl-4-(7-cyclopropylbenzo[b]thiophén-3-yl)-6-méthyl-1,4-dihydropyridine-3-carboxylate de méthyle 5-acétyl-2-cyclopropyl-6-méthyl-4-(thiéno[2,3-b]pyridin-3-yl)-1,4-dihydropyridine-3-carboxylate de 3-(pyridin-4-yl)benzyle 1,1'-(2,6-diméthyl-4-(5-(pyridin-3-yl)benzo[b]thiophén-3-yl)-1,4-dihydro-pyridine-3,5-diyl)diéthanone 5-acétyl-2-cyclopropyl-6-méthyl-4-(7-phénylbenzo[b]thiophén-3-yl)-1,4-dihydropyridine-3-carboxylate de méthyle. Composé comme défini dans l'une quelconque des revendications 1 à 13 pour une utilisation dans le traitement d'une maladie ou d'un état pathologique susceptible d'amélioration par antagonisme du récepteur d'androgène et/ou récepteur de glucocorticoïde choisi parmi le cancer de la prostate, cancer de la prostate résistant à la castration, cancer du pancréas, cancer de la vessie, cancer du rein, cancer gastrique, cancer du poumon, cancer du sein, cancer du colon, cancer colorectal, cancer des ovaires, et d'autres tumeurs solides, mélanome, cancers métastasés, hyperplasie de la prostate bénigne, syndrome de l'ovaire polycystique (PCOS), perte des cheveux, hirsutisme, acné, hypogonadisme, maladies atrophiant les muscles et cachexie, et syndrome de Cushing, prise de poids induite par des anti-psychotiques, obésité, trouble du stress post-traumatique et alcoolisme. Composition pharmaceutique comprenant un composé selon l'une quelconque des revendications 1 à 13 et un diluant ou support pharmaceutiquement acceptable. Composition pharmaceutique selon la revendication 15 comprenant de plus une quantité thérapeutiquement efficace d'un agent thérapeutique choisi parmi des agents pour traiter le cancer de la prostate, cancer de la prostate résistant à la castration, cancer du pancréas, cancer de la vessie, cancer du rein, cancer gastrique, cancer du poumon, cancer du sein, cancer du colon, cancer colorectal, cancer des ovaires, et d'autres tumeurs solides, mélanome, cancers métastasés, hyperplasie de la prostate bénigne, syndrome de l'ovaire polycystique (PCOS), perte des cheveux, hirsutisme, acné, hypogonadisme, maladies atrophiant les muscles et cachexie, et syndrome de Cushing, prise de poids induite par des anti-psychotiques, obésité, trouble du stress post-traumatique et alcoolisme. Produit de combinaison comprenant un composé selon l'une quelconque des revendications 1 à 13 et un agent thérapeutique utilisé pour le traitement du cancer de la prostate, cancer de la prostate résistant à la castration, cancer du pancréas, cancer de la vessie, cancer du rein, cancer gastrique, cancer du poumon, cancer du sein, cancer du colon, cancer colorectal, cancer des ovaires, et d'autres tumeurs solides, mélanome, cancers métastasés, hyperplasie de la prostate bénigne, syndrome de l'ovaire polycystique (PCOS), perte des cheveux, hirsutisme, acné, hypogonadisme, maladies atrophiant les muscles et cachexie, et syndrome de Cushing, prise de poids induite par des anti-psychotiques, obésité, trouble du stress post-traumatique et alcoolisme, en particulier un agent thérapeutique choisi parmi un agoniste ou antagoniste du récepteur de l'hormone libérant de la gonadotropine (GnRH), antagoniste du récepteur d'androgène, inhibiteur de CYP17, inhibiteur de VEGF, inhibiteur de EGFR, inhibiteur de PI3K, inhibiteur de AKT, inhibiteur de mTOR, inhibiteur de c-Met, inhibiteur de Src, inhibiteur de PARP, angiopoïétine, inhibiteur de ALK, inhibiteur de ROS-1, anticorps anti-(IGF), agent anti-néoplastique de taxane, inhibiteur de topoisomérase II, antibiotique anti-tumoral, inhibiteur de HSP90, inhibiteur de kinase Aurora, vaccin ciblant le PSA, antagonistes de GR, inhibiteurs de 11-bêta HSD, un ou pluieurs agents immunothérapeutiques choisis dans le groupe consistant en anticorps anti-CTLA4, anticorps anti-PD1 et anticorps anti-PDL1, en particulier un anticorps choisi dans le groupe consistant en ipilimumab, tremelimumab, nivolumab, pembrolizumab, CT-011, AMP-224, MPDL3280A, MEDI4736 et MDX-1105. REFERENCES CITED IN THE DESCRIPTION

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