(19)
(11) EP 3 775 850 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
19.01.2022 Bulletin 2022/03

(45) Mention of the grant of the patent:
22.09.2021 Bulletin 2021/38

(21) Application number: 19721082.6

(22) Date of filing: 26.03.2019
(51) International Patent Classification (IPC): 
G01N 21/64(2006.01)
G02B 21/16(2006.01)
(52) Cooperative Patent Classification (CPC):
G02B 21/16; G01N 21/6458
(86) International application number:
PCT/IB2019/052442
(87) International publication number:
WO 2019/186393 (03.10.2019 Gazette 2019/40)

(54)

SCATTERING-ASSISTED SUPER-LOCALIZATION MICROSCOPY METHOD AND RESPECTIVE APPARATUS

VERFFAHREN UND VORRICHTUNG FÜR DIE STREULICHTUNTERSTÜTZTE SUPERLOKALISIERUNGSMIKROSKOPIE

PROCÉDÉ ET DISPOSITIF POUR LA MICROSCOPIE À SUPERLOCALISATION SUPPORTÉE PAR LA DIFFUSION


(84) Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30) Priority: 27.03.2018 IT 201800003984

(43) Date of publication of application:
17.02.2021 Bulletin 2021/07

(73) Proprietors:
  • Disruptive Technological Advances In Life Science S.r.l.
    00165 Roma (RM) (IT)
  • Fondazione Istituto Italiano di Tecnologia
    16163 Genova (IT)

(72) Inventors:
  • LEONETTI, Marco
    16163 Genova (IT)
  • ANTONACCI, Giuseppe
    16163 Genova (IT)
  • CECCARELLI, Raino
    00167 Roma (IT)

(74) Representative: Papa, Elisabetta 
Società Italiana Brevetti S.p.A Piazza di Pietra, 39
00186 Roma
00186 Roma (IT)


(56) References cited: : 
   
  • IZEDDIN I ET AL: "PSF shaping using adaptive optics for three-dimensional single-molecule super-resolution imaging and tracking", OPTICS EXPRESS, vol. 20, no. 5, 13 February 2012 (2012-02-13), pages 4957-4967, XP055052832, ISSN: 1094-4087, DOI: 10.1364/OE.20.004957
  • THOMPSON M A ET AL: "Extending Microscopic Resolution with Single-Molecule Imaging and Active Control", ANNUAL REVIEW OF BIOPHYSICS, vol. 41, 9 June 2012 (2012-06-09), pages 321-342, XP055505598, ISSN: 1936-122X, DOI: 10.1146/annurev-biophys-050511-102250
  • DESCHOUT H ET AL: "Precisely and accurately localizing single emitters in fluorescence microscopy", NATURE METHODS, vol. 11, no. 3, 27 February 2014 (2014-02-27), pages 253-266, XP055447270, New York ISSN: 1548-7091, DOI: 10.1038/nmeth.2843
  • JI N ET AL: "Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues", NATURE METHODS, vol. 7, no. 2, 27 December 2009 (2009-12-27), pages 141-147, XP055138052, ISSN: 1548-7091, DOI: 10.1038/nmeth.1411 cited in the application
   
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).