(19)

(11)

EP 3 973 075 A1

(12)

(43) Date of publication: 30.03.2022 Bulletin 2022/13 (21) Application number: 20728173.4 (22) Date of filing: 22.05.2020

(51) International Patent Classification (IPC):  C12Q 1/6851(2018.01) C12Q 1/6834(2018.01) (52) Cooperative Patent Classification (CPC): C12Q 1/6851; C12Q 1/6834   C-Sets: C12Q 1/6851, C12Q 2521/131, C12Q 2525/186, C12Q 2527/119, C12Q 2537/157, C12Q 2565/301; C12Q 1/6834, C12Q 2521/131, C12Q 2525/186, C12Q 2527/119, C12Q 2537/157, C12Q 2565/301; (86) International application number: PCT/GB2020/051250 (87) International publication number: WO 2020/234605 (26.11.2020 Gazette 2020/48)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA ME Designated Validation States: KH MA MD TN (30) Priority: 22.05.2019 GB 201907209 (71) Applicant: Nuclera Nucleics Ltd Cambridge Cambridgeshire CB4 0GD (GB)	(72) Inventors: CHEN, Michael Chun Hao Cambridge CB4 0GD (GB) MCINROY, Gordon Ross Cambridge CB4 0GD (GB) (74) Representative: Stratagem IPM Limited  Meridian Court Comberton Road Toft, Cambridge CB23 2RY Toft, Cambridge CB23 2RY (GB)