               SEQUENCE LISTING

<110> Medizinische Hochschule Hannover

<120> All-in One vector for CAR and therapeutic effector molecule

<130> M1083PCT

<150> EP19181837.6
<151> 2019-06-21

<160> 35

<170> BiSSAP 1.3.6

<210> 1
<211> 244
<212> DNA
<213> Artificial Sequence


<220> 
<223> NFATenhsyn promoter

<220> 
<221> TATA_signal
<222> 202..208
<223> /note="TATA box"

<400> 1
tggaggaaaa actgtttcat acagaaggcg tggaggaaaa actgtttcat acagaaggcg     60

tggaggaaaa actgtttcat acagaaggcg tggaggaaaa actgtttcat acagaaggcg    120

tggaggaaaa actgtttcat acagaaggcg tggaggaaaa actgtttcat acagaaggcg    180

tctgcaggag actctagagg gtatataatg gtttaaactt aagcttggta ccgggccccc    240

gaag                                                                 244


<210> 2
<211> 364
<212> DNA
<213> Artificial Sequence


<220> 
<223> NFATmIL2 promoter

<220> 
<221> TATA_signal
<222> 291..297
<223> /note="TATA box"

<400> 2
gatatcgaat taggaggaaa aactgtttca tacagaaggc gtcaattagg aggaaaaact      60

gtttcataca gaaggcgtca attaggagga aaaactgttt catacagaag gcgtcaattg     120

gtcccatcga attaggagga aaaactgttt catacagaag gcgtcaatta ggaggaaaaa     180

ctgtttcata cagaaggcgt caattaggag gaaaaactgt ttcatacaga aggcgtcaat     240

tggtcccggg acattttgac acccccataa tatttttcca gaattaacag tataaattgc     300

atctcttgtt caagagttcc ctatcactct ctttaatcac tactcacagt aacctcaact     360

cctg                                                                  364


<210> 3
<211> 173
<212> DNA
<213> Artificial Sequence


<220> 
<223> NFκBenhsyn promoter

<220> 
<221> TATA_signal
<222> 131..137
<223> /note="TATA box"

<400> 3
ctcgagggga atttccgggg actttccggg aatttccggg gactttccgg gaatttccgg     60

gaatttccgg ggactttccg ggaatttccg gggactttcc gggaatttcc ctgcaggaga    120

ctctagaggg tatataatgg tttaaactta agcttggtac cgggcccccg aag           173


<210> 4
<211> 1623
<212> DNA
<213> Artificial Sequence


<220> 
<223> IL-12 coding sequence

<220> 
<221> CDS
<222> 1..1623
<223> /note="synthetic coding sequence for IL-12"
      /transl_table=1

<400> 4
atg tgt cac cag cag ctg gtc atc agc tgg ttc agc ctg gtg ttc ctg      48
Met Cys His Gln Gln Leu Val Ile Ser Trp Phe Ser Leu Val Phe Leu 
1               5                   10                  15      

gcc tct cct ctg gtg gcc atc tgg gag ctg aag aaa gac gtg tac gtg      96
Ala Ser Pro Leu Val Ala Ile Trp Glu Leu Lys Lys Asp Val Tyr Val 
            20                  25                  30          

gtg gaa ctg gac tgg tat ccc gat gct cct ggc gag atg gtg gtg ctg     144
Val Glu Leu Asp Trp Tyr Pro Asp Ala Pro Gly Glu Met Val Val Leu 
        35                  40                  45              

acc tgc gat acc cct gaa gag gac ggc atc acc tgg aca ctg gat cag     192
Thr Cys Asp Thr Pro Glu Glu Asp Gly Ile Thr Trp Thr Leu Asp Gln 
    50                  55                  60                  

tct agc gag gtg ctc ggc agc ggc aag acc ctg acc atc caa gtg aaa     240
Ser Ser Glu Val Leu Gly Ser Gly Lys Thr Leu Thr Ile Gln Val Lys 
65                  70                  75                  80  

gag ttt ggc gac gcc ggc cag tac acc tgt cac aaa ggc gga gaa gtg     288
Glu Phe Gly Asp Ala Gly Gln Tyr Thr Cys His Lys Gly Gly Glu Val 
                85                  90                  95      

ctg agc cac agc ctg ctg ctg ctc cac aag aaa gag gat ggc att tgg     336
Leu Ser His Ser Leu Leu Leu Leu His Lys Lys Glu Asp Gly Ile Trp 
            100                 105                 110         

agc acc gac atc ctg aag gac cag aaa gag ccc aag aac aag acc ttc     384
Ser Thr Asp Ile Leu Lys Asp Gln Lys Glu Pro Lys Asn Lys Thr Phe 
        115                 120                 125             

ctg aga tgc gag gcc aag aac tac agc ggc cgg ttc aca tgt tgg tgg     432
Leu Arg Cys Glu Ala Lys Asn Tyr Ser Gly Arg Phe Thr Cys Trp Trp 
    130                 135                 140                 

ctg acc acc atc agc acc gac ctg acc ttc agc gtg aag tcc agc aga     480
Leu Thr Thr Ile Ser Thr Asp Leu Thr Phe Ser Val Lys Ser Ser Arg 
145                 150                 155                 160 

ggc agc agt gat cct cag ggc gtt aca tgt ggc gcc gct aca ctg tct     528
Gly Ser Ser Asp Pro Gln Gly Val Thr Cys Gly Ala Ala Thr Leu Ser 
                165                 170                 175     

gcc gaa aga gtg cgg ggc gac aac aaa gaa tac gag tac agc gtg gaa     576
Ala Glu Arg Val Arg Gly Asp Asn Lys Glu Tyr Glu Tyr Ser Val Glu 
            180                 185                 190         

tgc caa gag gac agc gcc tgt cca gcc gcc gaa gag tct ctg cct atc     624
Cys Gln Glu Asp Ser Ala Cys Pro Ala Ala Glu Glu Ser Leu Pro Ile 
        195                 200                 205             

gaa gtg atg gtg gac gcc gtg cac aag ctg aag tac gag aac tac acc     672
Glu Val Met Val Asp Ala Val His Lys Leu Lys Tyr Glu Asn Tyr Thr 
    210                 215                 220                 

tcc agc ttt ttc atc cgg gac atc atc aag ccc gat cct cca aag aac     720
Ser Ser Phe Phe Ile Arg Asp Ile Ile Lys Pro Asp Pro Pro Lys Asn 
225                 230                 235                 240 

ctg cag ctg aag cct ctg aag aac agc aga cag gtg gaa gtg tcc tgg     768
Leu Gln Leu Lys Pro Leu Lys Asn Ser Arg Gln Val Glu Val Ser Trp 
                245                 250                 255     

gag tac ccc gac acc tgg tct aca ccc cac agc tac ttc agc ctg acc     816
Glu Tyr Pro Asp Thr Trp Ser Thr Pro His Ser Tyr Phe Ser Leu Thr 
            260                 265                 270         

ttt tgc gtg caa gtg cag ggc aag tcc aag cgc gag aaa aag gac cgg     864
Phe Cys Val Gln Val Gln Gly Lys Ser Lys Arg Glu Lys Lys Asp Arg 
        275                 280                 285             

gtg ttc acc gac aag acc agc gcc acc gtg atc tgc aga aag aac gcc     912
Val Phe Thr Asp Lys Thr Ser Ala Thr Val Ile Cys Arg Lys Asn Ala 
    290                 295                 300                 

agc atc agc gtc aga gcc cag gac cgc tac tac agc agc tct tgg agc     960
Ser Ile Ser Val Arg Ala Gln Asp Arg Tyr Tyr Ser Ser Ser Trp Ser 
305                 310                 315                 320 

gaa tgg gcc agc gtg cca tgt tct ggt ggc gga gga tct ggc gga ggt    1008
Glu Trp Ala Ser Val Pro Cys Ser Gly Gly Gly Gly Ser Gly Gly Gly 
                325                 330                 335     

gga agc ggc gga ggc gga tct aga aat ctg cct gtg gcc act cct gat    1056
Gly Ser Gly Gly Gly Gly Ser Arg Asn Leu Pro Val Ala Thr Pro Asp 
            340                 345                 350         

cct ggc atg ttc cct tgt ctg cac cac agc cag aac ctg ctg aga gcc    1104
Pro Gly Met Phe Pro Cys Leu His His Ser Gln Asn Leu Leu Arg Ala 
        355                 360                 365             

gtg tcc aac atg ctg cag aag gcc aga cag acc ctg gaa ttc tac ccc    1152
Val Ser Asn Met Leu Gln Lys Ala Arg Gln Thr Leu Glu Phe Tyr Pro 
    370                 375                 380                 

tgc acc agc gag gaa atc gac cac gag gac atc acc aag gat aag acc    1200
Cys Thr Ser Glu Glu Ile Asp His Glu Asp Ile Thr Lys Asp Lys Thr 
385                 390                 395                 400 

agt acc gtg gaa gcc tgc ctg cct ctg gaa ctg acc aag aac gag agc    1248
Ser Thr Val Glu Ala Cys Leu Pro Leu Glu Leu Thr Lys Asn Glu Ser 
                405                 410                 415     

tgc ctg aac agc cgg gaa acc agc ttc atc acc aac ggc tct tgc ctg    1296
Cys Leu Asn Ser Arg Glu Thr Ser Phe Ile Thr Asn Gly Ser Cys Leu 
            420                 425                 430         

gcc agc aga aag acc tcc ttc atg atg gcc ctg tgc ctg agc agc atc    1344
Ala Ser Arg Lys Thr Ser Phe Met Met Ala Leu Cys Leu Ser Ser Ile 
        435                 440                 445             

tac gag gac ctg aag atg tac cag gtg gaa ttc aag acc atg aac gcc    1392
Tyr Glu Asp Leu Lys Met Tyr Gln Val Glu Phe Lys Thr Met Asn Ala 
    450                 455                 460                 

aag ctg ctg atg gac ccc aag cgg cag atc ttc ctg gac cag aat atg    1440
Lys Leu Leu Met Asp Pro Lys Arg Gln Ile Phe Leu Asp Gln Asn Met 
465                 470                 475                 480 

ctg gcc gtg atc gac gag ctg atg cag gcc ctg aac ttc aac agc gag    1488
Leu Ala Val Ile Asp Glu Leu Met Gln Ala Leu Asn Phe Asn Ser Glu 
                485                 490                 495     

aca gtg ccc cag aag tct agc ctg gaa gaa ccc gac ttc tac aag acc    1536
Thr Val Pro Gln Lys Ser Ser Leu Glu Glu Pro Asp Phe Tyr Lys Thr 
            500                 505                 510         

aag atc aag ctg tgc atc ctg ctg cac gcc ttc cgc atc aga gcc gtg    1584
Lys Ile Lys Leu Cys Ile Leu Leu His Ala Phe Arg Ile Arg Ala Val 
        515                 520                 525             

acc atc gat aga gtg atg agc tac ctg aac gcc tcc tga                1623
Thr Ile Asp Arg Val Met Ser Tyr Leu Asn Ala Ser     
    530                 535                 540     

<210> 5
<211> 540
<212> PRT
<213> Artificial Sequence


<220> 
<223> [CDS]:1..1623 from SEQ ID NO 4

<400> 5
Met Cys His Gln Gln Leu Val Ile Ser Trp Phe Ser Leu Val Phe Leu 
1               5                   10                  15      
Ala Ser Pro Leu Val Ala Ile Trp Glu Leu Lys Lys Asp Val Tyr Val 
            20                  25                  30          
Val Glu Leu Asp Trp Tyr Pro Asp Ala Pro Gly Glu Met Val Val Leu 
        35                  40                  45              
Thr Cys Asp Thr Pro Glu Glu Asp Gly Ile Thr Trp Thr Leu Asp Gln 
    50                  55                  60                  
Ser Ser Glu Val Leu Gly Ser Gly Lys Thr Leu Thr Ile Gln Val Lys 
65                  70                  75                  80  
Glu Phe Gly Asp Ala Gly Gln Tyr Thr Cys His Lys Gly Gly Glu Val 
                85                  90                  95      
Leu Ser His Ser Leu Leu Leu Leu His Lys Lys Glu Asp Gly Ile Trp 
            100                 105                 110         
Ser Thr Asp Ile Leu Lys Asp Gln Lys Glu Pro Lys Asn Lys Thr Phe 
        115                 120                 125             
Leu Arg Cys Glu Ala Lys Asn Tyr Ser Gly Arg Phe Thr Cys Trp Trp 
    130                 135                 140                 
Leu Thr Thr Ile Ser Thr Asp Leu Thr Phe Ser Val Lys Ser Ser Arg 
145                 150                 155                 160 
Gly Ser Ser Asp Pro Gln Gly Val Thr Cys Gly Ala Ala Thr Leu Ser 
                165                 170                 175     
Ala Glu Arg Val Arg Gly Asp Asn Lys Glu Tyr Glu Tyr Ser Val Glu 
            180                 185                 190         
Cys Gln Glu Asp Ser Ala Cys Pro Ala Ala Glu Glu Ser Leu Pro Ile 
        195                 200                 205             
Glu Val Met Val Asp Ala Val His Lys Leu Lys Tyr Glu Asn Tyr Thr 
    210                 215                 220                 
Ser Ser Phe Phe Ile Arg Asp Ile Ile Lys Pro Asp Pro Pro Lys Asn 
225                 230                 235                 240 
Leu Gln Leu Lys Pro Leu Lys Asn Ser Arg Gln Val Glu Val Ser Trp 
                245                 250                 255     
Glu Tyr Pro Asp Thr Trp Ser Thr Pro His Ser Tyr Phe Ser Leu Thr 
            260                 265                 270         
Phe Cys Val Gln Val Gln Gly Lys Ser Lys Arg Glu Lys Lys Asp Arg 
        275                 280                 285             
Val Phe Thr Asp Lys Thr Ser Ala Thr Val Ile Cys Arg Lys Asn Ala 
    290                 295                 300                 
Ser Ile Ser Val Arg Ala Gln Asp Arg Tyr Tyr Ser Ser Ser Trp Ser 
305                 310                 315                 320 
Glu Trp Ala Ser Val Pro Cys Ser Gly Gly Gly Gly Ser Gly Gly Gly 
                325                 330                 335     
Gly Ser Gly Gly Gly Gly Ser Arg Asn Leu Pro Val Ala Thr Pro Asp 
            340                 345                 350         
Pro Gly Met Phe Pro Cys Leu His His Ser Gln Asn Leu Leu Arg Ala 
        355                 360                 365             
Val Ser Asn Met Leu Gln Lys Ala Arg Gln Thr Leu Glu Phe Tyr Pro 
    370                 375                 380                 
Cys Thr Ser Glu Glu Ile Asp His Glu Asp Ile Thr Lys Asp Lys Thr 
385                 390                 395                 400 
Ser Thr Val Glu Ala Cys Leu Pro Leu Glu Leu Thr Lys Asn Glu Ser 
                405                 410                 415     
Cys Leu Asn Ser Arg Glu Thr Ser Phe Ile Thr Asn Gly Ser Cys Leu 
            420                 425                 430         
Ala Ser Arg Lys Thr Ser Phe Met Met Ala Leu Cys Leu Ser Ser Ile 
        435                 440                 445             
Tyr Glu Asp Leu Lys Met Tyr Gln Val Glu Phe Lys Thr Met Asn Ala 
    450                 455                 460                 
Lys Leu Leu Met Asp Pro Lys Arg Gln Ile Phe Leu Asp Gln Asn Met 
465                 470                 475                 480 
Leu Ala Val Ile Asp Glu Leu Met Gln Ala Leu Asn Phe Asn Ser Glu 
                485                 490                 495     
Thr Val Pro Gln Lys Ser Ser Leu Glu Glu Pro Asp Phe Tyr Lys Thr 
            500                 505                 510         
Lys Ile Lys Leu Cys Ile Leu Leu His Ala Phe Arg Ile Arg Ala Val 
        515                 520                 525             
Thr Ile Asp Arg Val Met Ser Tyr Leu Asn Ala Ser 
    530                 535                 540 

<210> 6
<211> 534
<212> DNA
<213> Artificial Sequence


<220> 
<223> synthetic coding sequence for IL-18

<220> 
<221> CDS
<222> 1..534
<223> /note="synthetic coding sequence for IL-18"
      /transl_table=1

<400> 6
atg gcc tgg acc gtt ctc ctc ctc ggc ctc ctc tct cac tgc aca ggc     48
Met Ala Trp Thr Val Leu Leu Leu Gly Leu Leu Ser His Cys Thr Gly 
1               5                   10                  15      

tct gtg acc tcc tac ttt ggc aag ctt gaa tct aaa tta tca gtc ata     96
Ser Val Thr Ser Tyr Phe Gly Lys Leu Glu Ser Lys Leu Ser Val Ile 
            20                  25                  30          

aga aat ttg aat gac caa gtt ctc ttc att gac caa gga aat cgg cct    144
Arg Asn Leu Asn Asp Gln Val Leu Phe Ile Asp Gln Gly Asn Arg Pro 
        35                  40                  45              

cta ttt gaa gat atg act gat tct gac tgt aga gat aat gca ccc cgg    192
Leu Phe Glu Asp Met Thr Asp Ser Asp Cys Arg Asp Asn Ala Pro Arg 
    50                  55                  60                  

acc ata ttt att ata agt atg tat aaa gat agc cag cct aga ggt atg    240
Thr Ile Phe Ile Ile Ser Met Tyr Lys Asp Ser Gln Pro Arg Gly Met 
65                  70                  75                  80  

gct gta act atc tct gtg aag tgt gag aaa att tca act ctc tcc tgt    288
Ala Val Thr Ile Ser Val Lys Cys Glu Lys Ile Ser Thr Leu Ser Cys 
                85                  90                  95      

gag aac aaa att att tcc ttt aag gaa atg aat cct cct gat aac atc    336
Glu Asn Lys Ile Ile Ser Phe Lys Glu Met Asn Pro Pro Asp Asn Ile 
            100                 105                 110         

aag gat aca aaa agt gac atc ata ttc ttt cag aga agt gtc cca gga    384
Lys Asp Thr Lys Ser Asp Ile Ile Phe Phe Gln Arg Ser Val Pro Gly 
        115                 120                 125             

cat gat aat aag atg caa ttt gaa tct tca tca tac gaa gga tac ttt    432
His Asp Asn Lys Met Gln Phe Glu Ser Ser Ser Tyr Glu Gly Tyr Phe 
    130                 135                 140                 

cta gct tgt gaa aaa gag aga gac ctt ttt aaa ctc att ttg aaa aaa    480
Leu Ala Cys Glu Lys Glu Arg Asp Leu Phe Lys Leu Ile Leu Lys Lys 
145                 150                 155                 160 

gag gat gaa ttg ggg gat aga tct ata atg ttc act gtt caa aac gaa    528
Glu Asp Glu Leu Gly Asp Arg Ser Ile Met Phe Thr Val Gln Asn Glu 
                165                 170                 175     

gac tag                                                            534
Asp     
        

<210> 7
<211> 177
<212> PRT
<213> Artificial Sequence


<220> 
<223> [CDS]:1..534 from SEQ ID NO 6

<400> 7
Met Ala Trp Thr Val Leu Leu Leu Gly Leu Leu Ser His Cys Thr Gly 
1               5                   10                  15      
Ser Val Thr Ser Tyr Phe Gly Lys Leu Glu Ser Lys Leu Ser Val Ile 
            20                  25                  30          
Arg Asn Leu Asn Asp Gln Val Leu Phe Ile Asp Gln Gly Asn Arg Pro 
        35                  40                  45              
Leu Phe Glu Asp Met Thr Asp Ser Asp Cys Arg Asp Asn Ala Pro Arg 
    50                  55                  60                  
Thr Ile Phe Ile Ile Ser Met Tyr Lys Asp Ser Gln Pro Arg Gly Met 
65                  70                  75                  80  
Ala Val Thr Ile Ser Val Lys Cys Glu Lys Ile Ser Thr Leu Ser Cys 
                85                  90                  95      
Glu Asn Lys Ile Ile Ser Phe Lys Glu Met Asn Pro Pro Asp Asn Ile 
            100                 105                 110         
Lys Asp Thr Lys Ser Asp Ile Ile Phe Phe Gln Arg Ser Val Pro Gly 
        115                 120                 125             
His Asp Asn Lys Met Gln Phe Glu Ser Ser Ser Tyr Glu Gly Tyr Phe 
    130                 135                 140                 
Leu Ala Cys Glu Lys Glu Arg Asp Leu Phe Lys Leu Ile Leu Lys Lys 
145                 150                 155                 160 
Glu Asp Glu Leu Gly Asp Arg Ser Ile Met Phe Thr Val Gln Asn Glu 
                165                 170                 175     
Asp 
    

<210> 8
<211> 9555
<212> DNA
<213> Artificial Sequence


<220> 
<223> pCCL.PPT.NFATenhsyn.EGFP.PGK.CAR.wPRE

<220> 
<221> promoter
<222> 1785..2455
<223> /function="CMV promoter"

<220> 
<221> LTR
<222> 2456..2636
<223> /note="5´ LTR truncated"

<220> 
<221> misc_signal
<222> 2683..2808
<223> /note="packaging signal of HIV-1"

<220> 
<221> misc_signal
<222> 3301..3534
<223> /note="RRE of HIV-1"

<220> 
<221> misc_signal
<222> 3302..3543
<223> /note="PRE"

<220> 
<221> terminator
<222> 4027..4150
<223> /note="cPPT/CTScPPT/CTS, central polypurine tract and central
      termination sequence of HIV-1"

<220> 
<221> promoter
<222> 4174..4417
<223> /note="NFATenhsyn"

<220> 
<221> CDS
<222> 4430..5149
<223> /gene="EGFP"
      /transl_table=1

<220> 
<221> promoter
<222> 5158..5673
<223> /note="PGK promoter"

<220> 
<221> gene
<222> 5705..7159
<223> /gene="placeholder for CAR"

<220> 
<221> misc_signal
<222> 7186..7774
<223> /note="WPRE"

<220> 
<221> LTR
<222> 7861..8094
<223> /note="3´ LTR (∆ U3)"

<400> 8
agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca    60

gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag   120

gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc   180

gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg   240

tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc   300

ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg   360

cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg   420

gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga   480

cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac   540

tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa   600

aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca   660

aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag   720

gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac   780

cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa   840

ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc   900

accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag   960

tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac  1020

cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc  1080

gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc  1140

ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca  1200

cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc  1260

tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg  1320

ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct  1380

ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata  1440

ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc  1500

gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg  1560

acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca  1620

ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg  1680

tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa  1740

ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttggccattg catacgttgt  1800

atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac  1860

attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat  1920

atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg  1980

acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt  2040

tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag  2100

tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc  2160

attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag  2220

tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt  2280

ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc  2340

accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg  2400

gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccggggtc  2460

tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct  2520

taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga  2580

ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg  2640

cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact  2700

cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa  2760

attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg  2820

gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat  2880

aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc  2940

ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag  3000

acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat  3060

caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac  3120

aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat  3180

gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg  3240

agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat  3300

aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcctcaat  3360

gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt  3420

gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca  3480

gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat  3540

ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag  3600

taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat  3660

taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa  3720

gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat  3780

aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt  3840

aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt  3900

atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga  3960

agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta  4020

tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt  4080

agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca  4140

aaattttatc gatcacgaga ctagcctcga gcgtggagga aaaactgttt catacagaag  4200

gcgtggagga aaaactgttt catacagaag gcgtggagga aaaactgttt catacagaag  4260

gcgtggagga aaaactgttt catacagaag gcgtggagga aaaactgttt catacagaag  4320

gcgtggagga aaaactgttt catacagaag gcgtctgcag gagactctag agggtatata  4380

atggtttaaa cttaagcttg gtaccgggcc cccgaagacg cgtgccacc atg gtg agc  4438
                                                      Met Val Ser 
                                                      1           

aag ggc gag gag ctg ttc acc ggg gtg gtg ccc atc ctg gtc gag ctg    4486
Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu 
    5                   10                  15                  

gac ggc gac gta aac ggc cac aag ttc agc gtg tcc ggc gag ggc gag    4534
Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu 
20                  25                  30                  35  

ggc gat gcc acc tac ggc aag ctg acc ctg aag ttc atc tgc acc acc    4582
Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr 
                40                  45                  50      

ggc aag ctg ccc gtg ccc tgg ccc acc ctc gtg acc acc ctg acc tac    4630
Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr 
            55                  60                  65          

ggc gtg cag tgc ttc agc cgc tac ccc gac cac atg aag cag cac gac    4678
Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp 
        70                  75                  80              

ttc ttc aag tcc gcc atg ccc gaa ggc tac gtc cag gag cgc acc atc    4726
Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile 
    85                  90                  95                  

ttc ttc aag gac gac ggc aac tac aag acc cgc gcc gag gtg aag ttc    4774
Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe 
100                 105                 110                 115 

gag ggc gac acc ctg gtg aac cgc atc gag ctg aag ggc atc gac ttc    4822
Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe 
                120                 125                 130     

aag gag gac ggc aac atc ctg ggg cac aag ctg gag tac aac tac aac    4870
Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn 
            135                 140                 145         

agc cac aac gtc tat atc atg gcc gac aag cag aag aac ggc atc aag    4918
Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys 
        150                 155                 160             

gtg aac ttc aag atc cgc cac aac atc gag gac ggc agc gtg cag ctc    4966
Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu 
    165                 170                 175                 

gcc gac cac tac cag cag aac acc ccc atc ggc gac ggc ccc gtg ctg    5014
Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu 
180                 185                 190                 195 

ctg ccc gac aac cac tac ctg agc acc cag tcc gcc ctg agc aaa gac    5062
Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp 
                200                 205                 210     

ccc aac gag aag cgc gat cac atg gtc ctg ctg gag ttc gtg acc gcc    5110
Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala 
            215                 220                 225         

gcc ggg atc act ctc ggc atg gac gag ctg tac aag taa tcgaattccc     5159
Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys                
        230                 235                                

acggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc  5219

tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg  5279

tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt  5339

cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa  5399

acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca  5459

gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcggggcg cgccgagagc  5519

agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc  5579

ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc  5639

ctcgttgacc gaatcaccga cctctctccc cagggggatc caccggtcac gtggctagcg  5699

ccaccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5759

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5819

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5879

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5939

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5999

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6059

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6119

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6179

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6239

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6299

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6359

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6419

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6479

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6539

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6599

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6659

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6719

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6779

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6839

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6899

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6959

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  7019

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  7079

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  7139

nnnnnnnnnn nnnnnnnnnn taggatccgc cggcggccgc gtcgacaatc aacctctgga  7199

ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg  7259

tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt  7319

ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag  7379

gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc  7439

caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga  7499

actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa  7559

ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac  7619

ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct  7679

tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca  7739

gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta  7799

agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga  7859

ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct  7919

ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt  7979

aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac  8039

tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt  8099

agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt  8159

gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat  8219

ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat  8279

gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt  8339

ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct  8399

ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcgtcgaga  8459

cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca  8519

acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc  8579

tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg  8639

cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt  8699

ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc  8759

tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg  8819

gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta  8879

gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt  8939

ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat  8999

ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa  9059

tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc  9119

ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata  9179

cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga  9239

aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca  9299

ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat  9359

cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag  9419

agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc  9479

gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct  9539

cagaatgact tggttg                                                  9555

<210> 9
<211> 239
<212> PRT
<213> Artificial Sequence


<220> 
<223> [CDS]:4430..5149 from SEQ ID NO 8

<400> 9
Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      
Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          
Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              
Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  
Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  
Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      
Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         
Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             
Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 
Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 
Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     
Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         
Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 
Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 

<210> 10
<211> 407
<212> DNA
<213> Artificial Sequence


<220> 
<223> SFFV promoter

<400> 10
gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca      60

agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt     120

tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc     180

caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga     240

tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc     300

agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac     360

aacccctcac tcggcgcgcc agtcctccga cagactgagt cggccgg                   407


<210> 11
<211> 245
<212> DNA
<213> Artificial Sequence


<220> 
<223> EF-1 alpha promoter

<400> 11
tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg     60

gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag    120

tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc    180

agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtgtcgtg    240

acgcg                                                                245


<210> 12
<211> 671
<212> DNA
<213> Artificial Sequence


<220> 
<223> CMV promoter element

<400> 12
ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca      60

ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca     120

ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct     180

ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta     240

acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac     300

ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt     360

aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag     420

tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat     480

gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat     540

gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc     600

ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt     660

ttagtgaacc g                                                          671


<210> 13
<211> 232
<212> DNA
<213> Artificial Sequence


<220> 
<223> RSV promoter element

<400> 13
ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa     60

catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac    120

gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa    180

ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacaataa ac            232


<210> 14
<211> 454
<212> DNA
<213> Artificial Sequence


<220> 
<223> HIV U3 promoter element

<400> 14
ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac      60

acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca     120

ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc     180

aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg     240

gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga     300

gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc     360

gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga     420

tcctgcatat aagcagctgc tttttgcctg tact                                 454


<210> 15
<211> 589
<212> DNA
<213> Artificial Sequence


<220> 
<223> WPRE element

<400> 15
aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct      60

ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt     120

atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg     180

tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact     240

ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct     300

attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg     360

ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc     420

gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc     480

aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt     540

cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgc                 589


<210> 16
<211> 10469
<212> DNA
<213> Artificial Sequence


<220> 
<223> pCCL.PPT.NFATenhsyn.humanIL12.PGK.CAR.wPRE

<220> 
<221> promoter
<222> 1785..2455
<223> /note="CMV promoter"

<220> 
<221> LTR
<222> 2456..2636
<223> /note="5´ LTR truncated"

<220> 
<221> misc_signal
<222> 2683..2808
<223> /note="packaging signal of HIV-1"

<220> 
<221> misc_signal
<222> 3301..3534
<223> /note="RRE: rev response element of HIV-1"

<220> 
<221> misc_signal
<222> 3302..3543
<223> /note="PRE of HIV-1"

<220> 
<221> terminator
<222> 4027..4150
<223> /note="cPPT/CTScPPT/CTS: central polypurine tract and central
      termination sequence of HIV-1"

<220> 
<221> promoter
<222> 4174..4417
<223> /note="NFATenhsyn"

<220> 
<221> CDS
<222> 4430..6052
<223> /gene="sc IL2 humanized codon usage"
      /transl_table=1

<220> 
<221> promoter
<222> 6072..6587
<223> /note="PGK promoter"

<220> 
<221> gene
<222> 6619..8073
<223> /gene="placeholder for CAR"

<220> 
<221> LTR
<222> 7861..8094
<223> /note="3´ LTR (∆U3)"

<220> 
<221> misc_signal
<222> 8100..8688
<223> /note="WPRE"

<400> 16
agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca     60

gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag    120

gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc    180

gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg    240

tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc    300

ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg    360

cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg    420

gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga    480

cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac    540

tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa    600

aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca    660

aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag    720

gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac    780

cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa    840

ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc    900

accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag    960

tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac   1020

cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc   1080

gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc   1140

ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca   1200

cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc   1260

tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg   1320

ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct   1380

ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata   1440

ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc   1500

gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg   1560

acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca   1620

ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg   1680

tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa   1740

ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttggccattg catacgttgt   1800

atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac   1860

attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat   1920

atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg   1980

acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt   2040

tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag   2100

tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc   2160

attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag   2220

tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt   2280

ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc   2340

accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg   2400

gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccggggtc   2460

tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct   2520

taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga   2580

ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg   2640

cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact   2700

cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa   2760

attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg   2820

gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat   2880

aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc   2940

ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag   3000

acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat   3060

caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac   3120

aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat   3180

gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg   3240

agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat   3300

aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcctcaat   3360

gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt   3420

gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca   3480

gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat   3540

ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag   3600

taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat   3660

taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa   3720

gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat   3780

aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt   3840

aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt   3900

atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga   3960

agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta   4020

tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt   4080

agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca   4140

aaattttatc gatcacgaga ctagcctcga gcgtggagga aaaactgttt catacagaag   4200

gcgtggagga aaaactgttt catacagaag gcgtggagga aaaactgttt catacagaag   4260

gcgtggagga aaaactgttt catacagaag gcgtggagga aaaactgttt catacagaag   4320

gcgtggagga aaaactgttt catacagaag gcgtctgcag gagactctag agggtatata   4380

atggtttaaa cttaagcttg gtaccgggcc cccgaagacg cgtgccacc atg tgt cac   4438
                                                      Met Cys His 
                                                      1           

cag cag ctg gtc atc agc tgg ttc agc ctg gtg ttc ctg gcc tct cct     4486
Gln Gln Leu Val Ile Ser Trp Phe Ser Leu Val Phe Leu Ala Ser Pro 
    5                   10                  15                  

ctg gtg gcc atc tgg gag ctg aag aaa gac gtg tac gtg gtg gaa ctg     4534
Leu Val Ala Ile Trp Glu Leu Lys Lys Asp Val Tyr Val Val Glu Leu 
20                  25                  30                  35  

gac tgg tat ccc gat gct cct ggc gag atg gtg gtg ctg acc tgc gat     4582
Asp Trp Tyr Pro Asp Ala Pro Gly Glu Met Val Val Leu Thr Cys Asp 
                40                  45                  50      

acc cct gaa gag gac ggc atc acc tgg aca ctg gat cag tct agc gag     4630
Thr Pro Glu Glu Asp Gly Ile Thr Trp Thr Leu Asp Gln Ser Ser Glu 
            55                  60                  65          

gtg ctc ggc agc ggc aag acc ctg acc atc caa gtg aaa gag ttt ggc     4678
Val Leu Gly Ser Gly Lys Thr Leu Thr Ile Gln Val Lys Glu Phe Gly 
        70                  75                  80              

gac gcc ggc cag tac acc tgt cac aaa ggc gga gaa gtg ctg agc cac     4726
Asp Ala Gly Gln Tyr Thr Cys His Lys Gly Gly Glu Val Leu Ser His 
    85                  90                  95                  

agc ctg ctg ctg ctc cac aag aaa gag gat ggc att tgg agc acc gac     4774
Ser Leu Leu Leu Leu His Lys Lys Glu Asp Gly Ile Trp Ser Thr Asp 
100                 105                 110                 115 

atc ctg aag gac cag aaa gag ccc aag aac aag acc ttc ctg aga tgc     4822
Ile Leu Lys Asp Gln Lys Glu Pro Lys Asn Lys Thr Phe Leu Arg Cys 
                120                 125                 130     

gag gcc aag aac tac agc ggc cgg ttc aca tgt tgg tgg ctg acc acc     4870
Glu Ala Lys Asn Tyr Ser Gly Arg Phe Thr Cys Trp Trp Leu Thr Thr 
            135                 140                 145         

atc agc acc gac ctg acc ttc agc gtg aag tcc agc aga ggc agc agt     4918
Ile Ser Thr Asp Leu Thr Phe Ser Val Lys Ser Ser Arg Gly Ser Ser 
        150                 155                 160             

gat cct cag ggc gtt aca tgt ggc gcc gct aca ctg tct gcc gaa aga     4966
Asp Pro Gln Gly Val Thr Cys Gly Ala Ala Thr Leu Ser Ala Glu Arg 
    165                 170                 175                 

gtg cgg ggc gac aac aaa gaa tac gag tac agc gtg gaa tgc caa gag     5014
Val Arg Gly Asp Asn Lys Glu Tyr Glu Tyr Ser Val Glu Cys Gln Glu 
180                 185                 190                 195 

gac agc gcc tgt cca gcc gcc gaa gag tct ctg cct atc gaa gtg atg     5062
Asp Ser Ala Cys Pro Ala Ala Glu Glu Ser Leu Pro Ile Glu Val Met 
                200                 205                 210     

gtg gac gcc gtg cac aag ctg aag tac gag aac tac acc tcc agc ttt     5110
Val Asp Ala Val His Lys Leu Lys Tyr Glu Asn Tyr Thr Ser Ser Phe 
            215                 220                 225         

ttc atc cgg gac atc atc aag ccc gat cct cca aag aac ctg cag ctg     5158
Phe Ile Arg Asp Ile Ile Lys Pro Asp Pro Pro Lys Asn Leu Gln Leu 
        230                 235                 240             

aag cct ctg aag aac agc aga cag gtg gaa gtg tcc tgg gag tac ccc     5206
Lys Pro Leu Lys Asn Ser Arg Gln Val Glu Val Ser Trp Glu Tyr Pro 
    245                 250                 255                 

gac acc tgg tct aca ccc cac agc tac ttc agc ctg acc ttt tgc gtg     5254
Asp Thr Trp Ser Thr Pro His Ser Tyr Phe Ser Leu Thr Phe Cys Val 
260                 265                 270                 275 

caa gtg cag ggc aag tcc aag cgc gag aaa aag gac cgg gtg ttc acc     5302
Gln Val Gln Gly Lys Ser Lys Arg Glu Lys Lys Asp Arg Val Phe Thr 
                280                 285                 290     

gac aag acc agc gcc acc gtg atc tgc aga aag aac gcc agc atc agc     5350
Asp Lys Thr Ser Ala Thr Val Ile Cys Arg Lys Asn Ala Ser Ile Ser 
            295                 300                 305         

gtc aga gcc cag gac cgc tac tac agc agc tct tgg agc gaa tgg gcc     5398
Val Arg Ala Gln Asp Arg Tyr Tyr Ser Ser Ser Trp Ser Glu Trp Ala 
        310                 315                 320             

agc gtg cca tgt tct ggt ggc gga gga tct ggc gga ggt gga agc ggc     5446
Ser Val Pro Cys Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
    325                 330                 335                 

gga ggc gga tct aga aat ctg cct gtg gcc act cct gat cct ggc atg     5494
Gly Gly Gly Ser Arg Asn Leu Pro Val Ala Thr Pro Asp Pro Gly Met 
340                 345                 350                 355 

ttc cct tgt ctg cac cac agc cag aac ctg ctg aga gcc gtg tcc aac     5542
Phe Pro Cys Leu His His Ser Gln Asn Leu Leu Arg Ala Val Ser Asn 
                360                 365                 370     

atg ctg cag aag gcc aga cag acc ctg gaa ttc tac ccc tgc acc agc     5590
Met Leu Gln Lys Ala Arg Gln Thr Leu Glu Phe Tyr Pro Cys Thr Ser 
            375                 380                 385         

gag gaa atc gac cac gag gac atc acc aag gat aag acc agt acc gtg     5638
Glu Glu Ile Asp His Glu Asp Ile Thr Lys Asp Lys Thr Ser Thr Val 
        390                 395                 400             

gaa gcc tgc ctg cct ctg gaa ctg acc aag aac gag agc tgc ctg aac     5686
Glu Ala Cys Leu Pro Leu Glu Leu Thr Lys Asn Glu Ser Cys Leu Asn 
    405                 410                 415                 

agc cgg gaa acc agc ttc atc acc aac ggc tct tgc ctg gcc agc aga     5734
Ser Arg Glu Thr Ser Phe Ile Thr Asn Gly Ser Cys Leu Ala Ser Arg 
420                 425                 430                 435 

aag acc tcc ttc atg atg gcc ctg tgc ctg agc agc atc tac gag gac     5782
Lys Thr Ser Phe Met Met Ala Leu Cys Leu Ser Ser Ile Tyr Glu Asp 
                440                 445                 450     

ctg aag atg tac cag gtg gaa ttc aag acc atg aac gcc aag ctg ctg     5830
Leu Lys Met Tyr Gln Val Glu Phe Lys Thr Met Asn Ala Lys Leu Leu 
            455                 460                 465         

atg gac ccc aag cgg cag atc ttc ctg gac cag aat atg ctg gcc gtg     5878
Met Asp Pro Lys Arg Gln Ile Phe Leu Asp Gln Asn Met Leu Ala Val 
        470                 475                 480             

atc gac gag ctg atg cag gcc ctg aac ttc aac agc gag aca gtg ccc     5926
Ile Asp Glu Leu Met Gln Ala Leu Asn Phe Asn Ser Glu Thr Val Pro 
    485                 490                 495                 

cag aag tct agc ctg gaa gaa ccc gac ttc tac aag acc aag atc aag     5974
Gln Lys Ser Ser Leu Glu Glu Pro Asp Phe Tyr Lys Thr Lys Ile Lys 
500                 505                 510                 515 

ctg tgc atc ctg ctg cac gcc ttc cgc atc aga gcc gtg acc atc gat     6022
Leu Cys Ile Leu Leu His Ala Phe Arg Ile Arg Ala Val Thr Ile Asp 
                520                 525                 530     

aga gtg atg agc tac ctg aac gcc tcc tga tgtacaagta atcgaattcc       6072
Arg Val Met Ser Tyr Leu Asn Ala Ser                           
            535                 540                           

cacggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg   6132

ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac   6192

gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct   6252

tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca   6312

aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc   6372

agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcggggc gcgccgagag   6432

cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt   6492

cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc   6552

cctcgttgac cgaatcaccg acctctctcc ccagggggat ccaccggtca cgtggctagc   6612

gccaccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   6672

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   6732

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   6792

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   6852

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   6912

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   6972

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7032

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7092

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7152

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7212

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7272

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7332

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7392

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7452

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7512

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7572

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7632

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7692

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7752

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7812

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7872

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7932

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7992

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   8052

nnnnnnnnnn nnnnnnnnnn ntaggatccg ccggcggccg cgtcgacaat caacctctgg   8112

attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat   8172

gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt   8232

tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca   8292

ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg   8352

ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg   8412

aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca   8472

attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca   8532

cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc   8592

ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc   8652

agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattcgagct cggtaccttt   8712

aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg   8772

actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc   8832

tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct   8892

taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga   8952

ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag   9012

tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag   9072

tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa   9132

tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa   9192

tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat   9252

tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc   9312

tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcgtcgag   9372

acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac   9432

aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc   9492

ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc   9552

gcagcctgaa tggcgaatgg cgcgacgcgc cctgtagcgg cgcattaagc gcggcgggtg   9612

tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg   9672

ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg   9732

ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt   9792

agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt   9852

tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta   9912

tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa   9972

atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt  10032

cccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat  10092

acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg  10152

aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc  10212

attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga  10272

tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga  10332

gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg  10392

cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc  10452

tcagaatgac ttggttg                                                 10469

<210> 17
<211> 540
<212> PRT
<213> Artificial Sequence


<220> 
<223> [CDS]:4430..6052 from SEQ ID NO 16

<400> 17
Met Cys His Gln Gln Leu Val Ile Ser Trp Phe Ser Leu Val Phe Leu 
1               5                   10                  15      
Ala Ser Pro Leu Val Ala Ile Trp Glu Leu Lys Lys Asp Val Tyr Val 
            20                  25                  30          
Val Glu Leu Asp Trp Tyr Pro Asp Ala Pro Gly Glu Met Val Val Leu 
        35                  40                  45              
Thr Cys Asp Thr Pro Glu Glu Asp Gly Ile Thr Trp Thr Leu Asp Gln 
    50                  55                  60                  
Ser Ser Glu Val Leu Gly Ser Gly Lys Thr Leu Thr Ile Gln Val Lys 
65                  70                  75                  80  
Glu Phe Gly Asp Ala Gly Gln Tyr Thr Cys His Lys Gly Gly Glu Val 
                85                  90                  95      
Leu Ser His Ser Leu Leu Leu Leu His Lys Lys Glu Asp Gly Ile Trp 
            100                 105                 110         
Ser Thr Asp Ile Leu Lys Asp Gln Lys Glu Pro Lys Asn Lys Thr Phe 
        115                 120                 125             
Leu Arg Cys Glu Ala Lys Asn Tyr Ser Gly Arg Phe Thr Cys Trp Trp 
    130                 135                 140                 
Leu Thr Thr Ile Ser Thr Asp Leu Thr Phe Ser Val Lys Ser Ser Arg 
145                 150                 155                 160 
Gly Ser Ser Asp Pro Gln Gly Val Thr Cys Gly Ala Ala Thr Leu Ser 
                165                 170                 175     
Ala Glu Arg Val Arg Gly Asp Asn Lys Glu Tyr Glu Tyr Ser Val Glu 
            180                 185                 190         
Cys Gln Glu Asp Ser Ala Cys Pro Ala Ala Glu Glu Ser Leu Pro Ile 
        195                 200                 205             
Glu Val Met Val Asp Ala Val His Lys Leu Lys Tyr Glu Asn Tyr Thr 
    210                 215                 220                 
Ser Ser Phe Phe Ile Arg Asp Ile Ile Lys Pro Asp Pro Pro Lys Asn 
225                 230                 235                 240 
Leu Gln Leu Lys Pro Leu Lys Asn Ser Arg Gln Val Glu Val Ser Trp 
                245                 250                 255     
Glu Tyr Pro Asp Thr Trp Ser Thr Pro His Ser Tyr Phe Ser Leu Thr 
            260                 265                 270         
Phe Cys Val Gln Val Gln Gly Lys Ser Lys Arg Glu Lys Lys Asp Arg 
        275                 280                 285             
Val Phe Thr Asp Lys Thr Ser Ala Thr Val Ile Cys Arg Lys Asn Ala 
    290                 295                 300                 
Ser Ile Ser Val Arg Ala Gln Asp Arg Tyr Tyr Ser Ser Ser Trp Ser 
305                 310                 315                 320 
Glu Trp Ala Ser Val Pro Cys Ser Gly Gly Gly Gly Ser Gly Gly Gly 
                325                 330                 335     
Gly Ser Gly Gly Gly Gly Ser Arg Asn Leu Pro Val Ala Thr Pro Asp 
            340                 345                 350         
Pro Gly Met Phe Pro Cys Leu His His Ser Gln Asn Leu Leu Arg Ala 
        355                 360                 365             
Val Ser Asn Met Leu Gln Lys Ala Arg Gln Thr Leu Glu Phe Tyr Pro 
    370                 375                 380                 
Cys Thr Ser Glu Glu Ile Asp His Glu Asp Ile Thr Lys Asp Lys Thr 
385                 390                 395                 400 
Ser Thr Val Glu Ala Cys Leu Pro Leu Glu Leu Thr Lys Asn Glu Ser 
                405                 410                 415     
Cys Leu Asn Ser Arg Glu Thr Ser Phe Ile Thr Asn Gly Ser Cys Leu 
            420                 425                 430         
Ala Ser Arg Lys Thr Ser Phe Met Met Ala Leu Cys Leu Ser Ser Ile 
        435                 440                 445             
Tyr Glu Asp Leu Lys Met Tyr Gln Val Glu Phe Lys Thr Met Asn Ala 
    450                 455                 460                 
Lys Leu Leu Met Asp Pro Lys Arg Gln Ile Phe Leu Asp Gln Asn Met 
465                 470                 475                 480 
Leu Ala Val Ile Asp Glu Leu Met Gln Ala Leu Asn Phe Asn Ser Glu 
                485                 490                 495     
Thr Val Pro Gln Lys Ser Ser Leu Glu Glu Pro Asp Phe Tyr Lys Thr 
            500                 505                 510         
Lys Ile Lys Leu Cys Ile Leu Leu His Ala Phe Arg Ile Arg Ala Val 
        515                 520                 525             
Thr Ile Asp Arg Val Met Ser Tyr Leu Asn Ala Ser 
    530                 535                 540 

<210> 18
<211> 9380
<212> DNA
<213> Artificial Sequence


<220> 
<223> pCCL.PPT.NFATenhsyn.humanIL18.PGK.CAR.wPRE

<220> 
<221> promoter
<222> 1785..2455
<223> /note="CMV promoter"

<220> 
<221> LTR
<222> 2456..2636
<223> /note="5´ LTR truncated"

<220> 
<221> misc_signal
<222> 2683..2808
<223> /note="packaging signal of HIV-1"

<220> 
<221> misc_signal
<222> 3301..3534
<223> /note="RRE: rev response element of HIV-1"

<220> 
<221> terminator
<222> 4030..4147
<223> /note="cPPT/CTS, central polypurine tract and central termination
      sequence of HIV-1"

<220> 
<221> promoter
<222> 4174..4417
<223> /note="NFATenhsyn"

<220> 
<221> CDS
<222> 4430..4963
<223> /gene="signal peptide and human IL18"
      /transl_table=1

<220> 
<221> gene
<222> 5530..6984
<223> /note="placeholder for CAR"

<220> 
<221> misc_signal
<222> 7011..7599
<223> /note="WPRE"

<400> 18
agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca    60

gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag   120

gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc   180

gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg   240

tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc   300

ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg   360

cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg   420

gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga   480

cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac   540

tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa   600

aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca   660

aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag   720

gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac   780

cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa   840

ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc   900

accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag   960

tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac  1020

cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc  1080

gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc  1140

ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca  1200

cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc  1260

tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg  1320

ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct  1380

ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata  1440

ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc  1500

gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg  1560

acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca  1620

ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg  1680

tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa  1740

ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttggccattg catacgttgt  1800

atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac  1860

attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat  1920

atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg  1980

acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt  2040

tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag  2100

tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc  2160

attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag  2220

tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt  2280

ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc  2340

accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg  2400

gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccggggtc  2460

tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct  2520

taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga  2580

ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg  2640

cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact  2700

cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa  2760

attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg  2820

gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat  2880

aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc  2940

ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag  3000

acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat  3060

caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac  3120

aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat  3180

gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg  3240

agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat  3300

aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcctcaat  3360

gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt  3420

gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca  3480

gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat  3540

ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag  3600

taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat  3660

taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa  3720

gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat  3780

aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt  3840

aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt  3900

atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga  3960

agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta  4020

tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt  4080

agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca  4140

aaattttatc gatcacgaga ctagcctcga gcgtggagga aaaactgttt catacagaag  4200

gcgtggagga aaaactgttt catacagaag gcgtggagga aaaactgttt catacagaag  4260

gcgtggagga aaaactgttt catacagaag gcgtggagga aaaactgttt catacagaag  4320

gcgtggagga aaaactgttt catacagaag gcgtctgcag gagactctag agggtatata  4380

atggtttaaa cttaagcttg gtaccgggcc cccgaagacg cgtgccacc atg gcc tgg  4438
                                                      Met Ala Trp 
                                                      1           

acc gtt ctc ctc ctc ggc ctc ctc tct cac tgc aca ggc tct gtg acc    4486
Thr Val Leu Leu Leu Gly Leu Leu Ser His Cys Thr Gly Ser Val Thr 
    5                   10                  15                  

tcc tac ttt ggc aag ctt gaa tct aaa tta tca gtc ata aga aat ttg    4534
Ser Tyr Phe Gly Lys Leu Glu Ser Lys Leu Ser Val Ile Arg Asn Leu 
20                  25                  30                  35  

aat gac caa gtt ctc ttc att gac caa gga aat cgg cct cta ttt gaa    4582
Asn Asp Gln Val Leu Phe Ile Asp Gln Gly Asn Arg Pro Leu Phe Glu 
                40                  45                  50      

gat atg act gat tct gac tgt aga gat aat gca ccc cgg acc ata ttt    4630
Asp Met Thr Asp Ser Asp Cys Arg Asp Asn Ala Pro Arg Thr Ile Phe 
            55                  60                  65          

att ata agt atg tat aaa gat agc cag cct aga ggt atg gct gta act    4678
Ile Ile Ser Met Tyr Lys Asp Ser Gln Pro Arg Gly Met Ala Val Thr 
        70                  75                  80              

atc tct gtg aag tgt gag aaa att tca act ctc tcc tgt gag aac aaa    4726
Ile Ser Val Lys Cys Glu Lys Ile Ser Thr Leu Ser Cys Glu Asn Lys 
    85                  90                  95                  

att att tcc ttt aag gaa atg aat cct cct gat aac atc aag gat aca    4774
Ile Ile Ser Phe Lys Glu Met Asn Pro Pro Asp Asn Ile Lys Asp Thr 
100                 105                 110                 115 

aaa agt gac atc ata ttc ttt cag aga agt gtc cca gga cat gat aat    4822
Lys Ser Asp Ile Ile Phe Phe Gln Arg Ser Val Pro Gly His Asp Asn 
                120                 125                 130     

aag atg caa ttt gaa tct tca tca tac gaa gga tac ttt cta gct tgt    4870
Lys Met Gln Phe Glu Ser Ser Ser Tyr Glu Gly Tyr Phe Leu Ala Cys 
            135                 140                 145         

gaa aaa gag aga gac ctt ttt aaa ctc att ttg aaa aaa gag gat gaa    4918
Glu Lys Glu Arg Asp Leu Phe Lys Leu Ile Leu Lys Lys Glu Asp Glu 
        150                 155                 160             

ttg ggg gat aga tct ata atg ttc act gtt caa aac gaa gac tag        4963
Leu Gly Asp Arg Ser Ile Met Phe Thr Val Gln Asn Glu Asp     
    165                 170                 175             

tgtacaagta atcgaattcc cacggggttg gggttgcgcc ttttccaagg cagccctggg  5023

tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct  5083

gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt  5143

gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc  5203

gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga  5263

cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc  5323

tcagcggggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg  5383

gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag  5443

cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggat  5503

ccaccggtca cgtggctagc gccaccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5563

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5623

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5683

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5743

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5803

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5863

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5923

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5983

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6043

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6103

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6163

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6223

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6283

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6343

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6403

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6463

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6523

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6583

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6643

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6703

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6763

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6823

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6883

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6943

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntaggatccg ccggcggccg  7003

cgtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta  7063

tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc  7123

ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga  7183

ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac  7243

ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc  7303

cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc  7363

tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg  7423

gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc  7483

ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc  7543

gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg  7603

aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac  7663

tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg  7723

ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc  7783

taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg  7843

tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg  7903

tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca  7963

aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa  8023

taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt  8083

ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa  8143

ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag  8203

aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag  8263

gcctaggctt ttgcgtcgag acgtacccaa ttcgccctat agtgagtcgt attacgcgcg  8323

ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa  8383

tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga  8443

tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcgacgcgc cctgtagcgg  8503

cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc  8563

cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc  8623

ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct  8683

cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac  8743

ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac  8803

tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat  8863

ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa  8923

aatattaacg tttacaattt cccaggtggc acttttcggg gaaatgtgcg cggaacccct  8983

atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga  9043

taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc  9103

cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg  9163

aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc  9223

aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact  9283

tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc  9343

ggtcgccgca tacactattc tcagaatgac ttggttg                           9380

<210> 19
<211> 177
<212> PRT
<213> Artificial Sequence


<220> 
<223> [CDS]:4430..4963 from SEQ ID NO 18

<400> 19
Met Ala Trp Thr Val Leu Leu Leu Gly Leu Leu Ser His Cys Thr Gly 
1               5                   10                  15      
Ser Val Thr Ser Tyr Phe Gly Lys Leu Glu Ser Lys Leu Ser Val Ile 
            20                  25                  30          
Arg Asn Leu Asn Asp Gln Val Leu Phe Ile Asp Gln Gly Asn Arg Pro 
        35                  40                  45              
Leu Phe Glu Asp Met Thr Asp Ser Asp Cys Arg Asp Asn Ala Pro Arg 
    50                  55                  60                  
Thr Ile Phe Ile Ile Ser Met Tyr Lys Asp Ser Gln Pro Arg Gly Met 
65                  70                  75                  80  
Ala Val Thr Ile Ser Val Lys Cys Glu Lys Ile Ser Thr Leu Ser Cys 
                85                  90                  95      
Glu Asn Lys Ile Ile Ser Phe Lys Glu Met Asn Pro Pro Asp Asn Ile 
            100                 105                 110         
Lys Asp Thr Lys Ser Asp Ile Ile Phe Phe Gln Arg Ser Val Pro Gly 
        115                 120                 125             
His Asp Asn Lys Met Gln Phe Glu Ser Ser Ser Tyr Glu Gly Tyr Phe 
    130                 135                 140                 
Leu Ala Cys Glu Lys Glu Arg Asp Leu Phe Lys Leu Ile Leu Lys Lys 
145                 150                 155                 160 
Glu Asp Glu Leu Gly Asp Arg Ser Ile Met Phe Thr Val Gln Asn Glu 
                165                 170                 175     
Asp 
    

<210> 20
<211> 9684
<212> DNA
<213> Artificial Sequence


<220> 
<223> pCCL.PPT.NFATmIL2.EGFP.PGK.CAR.wPRE

<220> 
<221> misc_signal
<222> 2683..2808
<223> /note="HIV-1 packaging signal"

<220> 
<221> misc_feature
<222> 3302..3543
<223> /note="RRE: rev response element of HIV-1"

<220> 
<221> misc_feature
<222> 4030..4147
<223> /note="cPPT/CTScPPT/CTS: central polypurine tract and central
      termination sequence of HIV-1"

<220> 
<221> promoter
<222> 4178..4414
<223> /note="6x NFAT"

<220> 
<221> promoter
<222> 4421..4540
<223> /note="mIL2 promoter including TATA box"

<220> 
<221> CDS
<222> 4559..5278
<223> /note="eGFP"
      /transl_table=1

<220> 
<221> promoter
<222> 5287..5802
<223> /note="PGK promoter"

<220> 
<221> gene
<222> 5834..7288
<223> /gene="placeholder for CAR"

<220> 
<221> misc_signal
<222> 7315..7903
<223> /note="wPRE: woodchuck hepatitis virus posttranscriptional
      regulatory element"

<400> 20
agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca    60

gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag   120

gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc   180

gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg   240

tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc   300

ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg   360

cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg   420

gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga   480

cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac   540

tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa   600

aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca   660

aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag   720

gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac   780

cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa   840

ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc   900

accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag   960

tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac  1020

cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc  1080

gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc  1140

ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca  1200

cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc  1260

tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg  1320

ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct  1380

ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata  1440

ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc  1500

gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg  1560

acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca  1620

ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg  1680

tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa  1740

ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttggccattg catacgttgt  1800

atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac  1860

attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat  1920

atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg  1980

acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt  2040

tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag  2100

tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc  2160

attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag  2220

tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt  2280

ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc  2340

accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg  2400

gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccggggtc  2460

tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct  2520

taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga  2580

ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg  2640

cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact  2700

cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa  2760

attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg  2820

gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat  2880

aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc  2940

ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag  3000

acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat  3060

caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac  3120

aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat  3180

gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg  3240

agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat  3300

aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcctcaat  3360

gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt  3420

gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca  3480

gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat  3540

ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag  3600

taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat  3660

taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa  3720

gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat  3780

aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt  3840

aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt  3900

atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga  3960

agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta  4020

tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt  4080

agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca  4140

aaattttatc gatcacgaga ctagcctcga cagcgctgat atcgaattag gaggaaaaac  4200

tgtttcatac agaaggcgtc aattaggagg aaaaactgtt tcatacagaa ggcgtcaatt  4260

aggaggaaaa actgtttcat acagaaggcg tcaattggtc ccatcgaatt aggaggaaaa  4320

actgtttcat acagaaggcg tcaattagga ggaaaaactg tttcatacag aaggcgtcaa  4380

ttaggaggaa aaactgtttc atacagaagg cgtcaattgg tcccgggaca ttttgacacc  4440

cccataatat ttttccagaa ttaacagtat aaattgcatc tcttgttcaa gagttcccta  4500

tcactctctt taatcactac tcacagtaac ctcaactcct ggatccacgc gtgccacc    4558

atg gtg agc aag ggc gag gag ctg ttc acc ggg gtg gtg ccc atc ctg    4606
Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      

gtc gag ctg gac ggc gac gta aac ggc cac aag ttc agc gtg tcc ggc    4654
Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          

gag ggc gag ggc gat gcc acc tac ggc aag ctg acc ctg aag ttc atc    4702
Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              

tgc acc acc ggc aag ctg ccc gtg ccc tgg ccc acc ctc gtg acc acc    4750
Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  

ctg acc tac ggc gtg cag tgc ttc agc cgc tac ccc gac cac atg aag    4798
Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  

cag cac gac ttc ttc aag tcc gcc atg ccc gaa ggc tac gtc cag gag    4846
Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      

cgc acc atc ttc ttc aag gac gac ggc aac tac aag acc cgc gcc gag    4894
Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         

gtg aag ttc gag ggc gac acc ctg gtg aac cgc atc gag ctg aag ggc    4942
Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             

atc gac ttc aag gag gac ggc aac atc ctg ggg cac aag ctg gag tac    4990
Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 

aac tac aac agc cac aac gtc tat atc atg gcc gac aag cag aag aac    5038
Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 

ggc atc aag gtg aac ttc aag atc cgc cac aac atc gag gac ggc agc    5086
Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     

gtg cag ctc gcc gac cac tac cag cag aac acc ccc atc ggc gac ggc    5134
Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         

ccc gtg ctg ctg ccc gac aac cac tac ctg agc acc cag tcc gcc ctg    5182
Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             

agc aaa gac ccc aac gag aag cgc gat cac atg gtc ctg ctg gag ttc    5230
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 

gtg acc gcc gcc ggg atc act ctc ggc atg gac gag ctg tac aag taa    5278
Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys     
225                 230                 235                     

tcgaattccc acggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg  5338

acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac  5398

attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc  5458

ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg  5518

gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg  5578

agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcggggcg  5638

cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg  5698

ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc  5758

agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggatc caccggtcac  5818

gtggctagcg ccaccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5878

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5938

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5998

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6058

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6118

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6178

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6238

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6298

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6358

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6418

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6478

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6538

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6598

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6658

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6718

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6778

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6838

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6898

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6958

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  7018

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  7078

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  7138

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  7198

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  7258

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn taggatccgc cggcggccgc gtcgacaatc  7318

aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt  7378

ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg  7438

ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc  7498

ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt  7558

ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg  7618

ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg  7678

gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct  7738

gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc  7798

cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc  7858

ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc  7918

ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga  7978

aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg  8038

tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa  8098

cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct  8158

gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc  8218

tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat  8278

atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag  8338

catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa  8398

actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt  8458

tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc  8518

gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt  8578

tgcgtcgaga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg  8638

tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag  8698

cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc  8758

aacagttgcg cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg  8818

cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg  8878

ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc  8938

taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa  8998

aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc  9058

ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac  9118

tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt  9178

ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt  9238

ttacaatttc ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt  9298

tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca  9358

ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt  9418

ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga  9478

tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa  9538

gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct  9598

gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat  9658

acactattct cagaatgact tggttg                                       9684

<210> 21
<211> 239
<212> PRT
<213> Artificial Sequence


<220> 
<223> [CDS]:4559..5278 from SEQ ID NO 20

<400> 21
Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      
Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          
Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              
Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  
Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  
Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      
Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         
Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             
Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 
Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 
Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     
Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         
Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 
Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 

<210> 22
<211> 10598
<212> DNA
<213> Artificial Sequence


<220> 
<223> pCCL.PPT.NFATmIL2.humanIL12.PGK.CAR.wPRE

<220> 
<221> misc_signal
<222> 2683..2808
<223> /note="HIV-1 psi: packaging signal of human immunodeficiency
      virus type 1"

<220> 
<221> misc_signal
<222> 3302..3543
<223> /note="RRE: rev response element of HIV-1"

<220> 
<221> misc_feature
<222> 4030..4147
<223> /note="cPPT/CTScPPT/CTS: central polypurine tract and central
      termination sequence of HIV-1"

<220> 
<221> promoter
<222> 4178..4414
<223> /note="6 x NFAT"

<220> 
<221> promoter
<222> 4421..4540
<223> /note="mIL2 promoter"

<220> 
<221> CDS
<222> 4559..6181
<223> /gene="sc Il12 humanized codon usage"
      /transl_table=1

<220> 
<221> promoter
<222> 6201..6716
<223> /note="PGK promoter"

<220> 
<221> gene
<222> 6748..8202
<223> /gene="placeholder for CAR"

<220> 
<221> misc_signal
<222> 8229..8817
<223> /note="wPRE: woodchuck hepatitis virus posttranscriptional
      regulatory element"

<400> 22
agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca     60

gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag    120

gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc    180

gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg    240

tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc    300

ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg    360

cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg    420

gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga    480

cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac    540

tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa    600

aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca    660

aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag    720

gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac    780

cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa    840

ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc    900

accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag    960

tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac   1020

cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc   1080

gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc   1140

ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca   1200

cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc   1260

tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg   1320

ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct   1380

ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata   1440

ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc   1500

gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg   1560

acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca   1620

ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg   1680

tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa   1740

ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttggccattg catacgttgt   1800

atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac   1860

attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat   1920

atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg   1980

acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt   2040

tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag   2100

tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc   2160

attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag   2220

tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt   2280

ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc   2340

accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg   2400

gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccggggtc   2460

tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct   2520

taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga   2580

ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg   2640

cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact   2700

cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa   2760

attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg   2820

gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat   2880

aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc   2940

ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag   3000

acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat   3060

caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac   3120

aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat   3180

gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg   3240

agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat   3300

aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcctcaat   3360

gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt   3420

gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca   3480

gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat   3540

ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag   3600

taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat   3660

taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa   3720

gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat   3780

aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt   3840

aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt   3900

atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga   3960

agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta   4020

tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt   4080

agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca   4140

aaattttatc gatcacgaga ctagcctcga cagcgctgat atcgaattag gaggaaaaac   4200

tgtttcatac agaaggcgtc aattaggagg aaaaactgtt tcatacagaa ggcgtcaatt   4260

aggaggaaaa actgtttcat acagaaggcg tcaattggtc ccatcgaatt aggaggaaaa   4320

actgtttcat acagaaggcg tcaattagga ggaaaaactg tttcatacag aaggcgtcaa   4380

ttaggaggaa aaactgtttc atacagaagg cgtcaattgg tcccgggaca ttttgacacc   4440

cccataatat ttttccagaa ttaacagtat aaattgcatc tcttgttcaa gagttcccta   4500

tcactctctt taatcactac tcacagtaac ctcaactcct ggatccacgc gtgccacc     4558

atg tgt cac cag cag ctg gtc atc agc tgg ttc agc ctg gtg ttc ctg     4606
Met Cys His Gln Gln Leu Val Ile Ser Trp Phe Ser Leu Val Phe Leu 
1               5                   10                  15      

gcc tct cct ctg gtg gcc atc tgg gag ctg aag aaa gac gtg tac gtg     4654
Ala Ser Pro Leu Val Ala Ile Trp Glu Leu Lys Lys Asp Val Tyr Val 
            20                  25                  30          

gtg gaa ctg gac tgg tat ccc gat gct cct ggc gag atg gtg gtg ctg     4702
Val Glu Leu Asp Trp Tyr Pro Asp Ala Pro Gly Glu Met Val Val Leu 
        35                  40                  45              

acc tgc gat acc cct gaa gag gac ggc atc acc tgg aca ctg gat cag     4750
Thr Cys Asp Thr Pro Glu Glu Asp Gly Ile Thr Trp Thr Leu Asp Gln 
    50                  55                  60                  

tct agc gag gtg ctc ggc agc ggc aag acc ctg acc atc caa gtg aaa     4798
Ser Ser Glu Val Leu Gly Ser Gly Lys Thr Leu Thr Ile Gln Val Lys 
65                  70                  75                  80  

gag ttt ggc gac gcc ggc cag tac acc tgt cac aaa ggc gga gaa gtg     4846
Glu Phe Gly Asp Ala Gly Gln Tyr Thr Cys His Lys Gly Gly Glu Val 
                85                  90                  95      

ctg agc cac agc ctg ctg ctg ctc cac aag aaa gag gat ggc att tgg     4894
Leu Ser His Ser Leu Leu Leu Leu His Lys Lys Glu Asp Gly Ile Trp 
            100                 105                 110         

agc acc gac atc ctg aag gac cag aaa gag ccc aag aac aag acc ttc     4942
Ser Thr Asp Ile Leu Lys Asp Gln Lys Glu Pro Lys Asn Lys Thr Phe 
        115                 120                 125             

ctg aga tgc gag gcc aag aac tac agc ggc cgg ttc aca tgt tgg tgg     4990
Leu Arg Cys Glu Ala Lys Asn Tyr Ser Gly Arg Phe Thr Cys Trp Trp 
    130                 135                 140                 

ctg acc acc atc agc acc gac ctg acc ttc agc gtg aag tcc agc aga     5038
Leu Thr Thr Ile Ser Thr Asp Leu Thr Phe Ser Val Lys Ser Ser Arg 
145                 150                 155                 160 

ggc agc agt gat cct cag ggc gtt aca tgt ggc gcc gct aca ctg tct     5086
Gly Ser Ser Asp Pro Gln Gly Val Thr Cys Gly Ala Ala Thr Leu Ser 
                165                 170                 175     

gcc gaa aga gtg cgg ggc gac aac aaa gaa tac gag tac agc gtg gaa     5134
Ala Glu Arg Val Arg Gly Asp Asn Lys Glu Tyr Glu Tyr Ser Val Glu 
            180                 185                 190         

tgc caa gag gac agc gcc tgt cca gcc gcc gaa gag tct ctg cct atc     5182
Cys Gln Glu Asp Ser Ala Cys Pro Ala Ala Glu Glu Ser Leu Pro Ile 
        195                 200                 205             

gaa gtg atg gtg gac gcc gtg cac aag ctg aag tac gag aac tac acc     5230
Glu Val Met Val Asp Ala Val His Lys Leu Lys Tyr Glu Asn Tyr Thr 
    210                 215                 220                 

tcc agc ttt ttc atc cgg gac atc atc aag ccc gat cct cca aag aac     5278
Ser Ser Phe Phe Ile Arg Asp Ile Ile Lys Pro Asp Pro Pro Lys Asn 
225                 230                 235                 240 

ctg cag ctg aag cct ctg aag aac agc aga cag gtg gaa gtg tcc tgg     5326
Leu Gln Leu Lys Pro Leu Lys Asn Ser Arg Gln Val Glu Val Ser Trp 
                245                 250                 255     

gag tac ccc gac acc tgg tct aca ccc cac agc tac ttc agc ctg acc     5374
Glu Tyr Pro Asp Thr Trp Ser Thr Pro His Ser Tyr Phe Ser Leu Thr 
            260                 265                 270         

ttt tgc gtg caa gtg cag ggc aag tcc aag cgc gag aaa aag gac cgg     5422
Phe Cys Val Gln Val Gln Gly Lys Ser Lys Arg Glu Lys Lys Asp Arg 
        275                 280                 285             

gtg ttc acc gac aag acc agc gcc acc gtg atc tgc aga aag aac gcc     5470
Val Phe Thr Asp Lys Thr Ser Ala Thr Val Ile Cys Arg Lys Asn Ala 
    290                 295                 300                 

agc atc agc gtc aga gcc cag gac cgc tac tac agc agc tct tgg agc     5518
Ser Ile Ser Val Arg Ala Gln Asp Arg Tyr Tyr Ser Ser Ser Trp Ser 
305                 310                 315                 320 

gaa tgg gcc agc gtg cca tgt tct ggt ggc gga gga tct ggc gga ggt     5566
Glu Trp Ala Ser Val Pro Cys Ser Gly Gly Gly Gly Ser Gly Gly Gly 
                325                 330                 335     

gga agc ggc gga ggc gga tct aga aat ctg cct gtg gcc act cct gat     5614
Gly Ser Gly Gly Gly Gly Ser Arg Asn Leu Pro Val Ala Thr Pro Asp 
            340                 345                 350         

cct ggc atg ttc cct tgt ctg cac cac agc cag aac ctg ctg aga gcc     5662
Pro Gly Met Phe Pro Cys Leu His His Ser Gln Asn Leu Leu Arg Ala 
        355                 360                 365             

gtg tcc aac atg ctg cag aag gcc aga cag acc ctg gaa ttc tac ccc     5710
Val Ser Asn Met Leu Gln Lys Ala Arg Gln Thr Leu Glu Phe Tyr Pro 
    370                 375                 380                 

tgc acc agc gag gaa atc gac cac gag gac atc acc aag gat aag acc     5758
Cys Thr Ser Glu Glu Ile Asp His Glu Asp Ile Thr Lys Asp Lys Thr 
385                 390                 395                 400 

agt acc gtg gaa gcc tgc ctg cct ctg gaa ctg acc aag aac gag agc     5806
Ser Thr Val Glu Ala Cys Leu Pro Leu Glu Leu Thr Lys Asn Glu Ser 
                405                 410                 415     

tgc ctg aac agc cgg gaa acc agc ttc atc acc aac ggc tct tgc ctg     5854
Cys Leu Asn Ser Arg Glu Thr Ser Phe Ile Thr Asn Gly Ser Cys Leu 
            420                 425                 430         

gcc agc aga aag acc tcc ttc atg atg gcc ctg tgc ctg agc agc atc     5902
Ala Ser Arg Lys Thr Ser Phe Met Met Ala Leu Cys Leu Ser Ser Ile 
        435                 440                 445             

tac gag gac ctg aag atg tac cag gtg gaa ttc aag acc atg aac gcc     5950
Tyr Glu Asp Leu Lys Met Tyr Gln Val Glu Phe Lys Thr Met Asn Ala 
    450                 455                 460                 

aag ctg ctg atg gac ccc aag cgg cag atc ttc ctg gac cag aat atg     5998
Lys Leu Leu Met Asp Pro Lys Arg Gln Ile Phe Leu Asp Gln Asn Met 
465                 470                 475                 480 

ctg gcc gtg atc gac gag ctg atg cag gcc ctg aac ttc aac agc gag     6046
Leu Ala Val Ile Asp Glu Leu Met Gln Ala Leu Asn Phe Asn Ser Glu 
                485                 490                 495     

aca gtg ccc cag aag tct agc ctg gaa gaa ccc gac ttc tac aag acc     6094
Thr Val Pro Gln Lys Ser Ser Leu Glu Glu Pro Asp Phe Tyr Lys Thr 
            500                 505                 510         

aag atc aag ctg tgc atc ctg ctg cac gcc ttc cgc atc aga gcc gtg     6142
Lys Ile Lys Leu Cys Ile Leu Leu His Ala Phe Arg Ile Arg Ala Val 
        515                 520                 525             

acc atc gat aga gtg atg agc tac ctg aac gcc tcc tga tgtacaagta      6191
Thr Ile Asp Arg Val Met Ser Tyr Leu Asn Ala Ser                
    530                 535                 540                

atcgaattcc cacggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg   6251

gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca   6311

cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc   6371

cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc   6431

ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg   6491

gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcggggc   6551

gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt   6611

gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg   6671

cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggat ccaccggtca   6731

cgtggctagc gccaccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   6791

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   6851

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   6911

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   6971

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7031

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7091

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7151

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7211

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7271

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7331

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7391

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7451

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7511

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7571

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7631

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7691

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7751

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7811

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7871

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7931

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   7991

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   8051

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   8111

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn   8171

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntaggatccg ccggcggccg cgtcgacaat   8231

caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct   8291

tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg   8351

gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg   8411

cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt   8471

tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt   8531

gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg   8591

ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc   8651

tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat   8711

ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc   8771

cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattcgagct   8831

cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag   8891

aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt   8951

gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga   9011

acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc   9071

tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct   9131

ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa   9191

tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata   9251

gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca   9311

aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag   9371

ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc   9431

cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt   9491

ttgcgtcgag acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc   9551

gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca   9611

gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc   9671

caacagttgc gcagcctgaa tggcgaatgg cgcgacgcgc cctgtagcgg cgcattaagc   9731

gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc   9791

gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct   9851

ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa   9911

aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc   9971

cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca  10031

ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat  10091

tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg  10151

tttacaattt cccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat  10211

ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc  10271

aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct  10331

tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag  10391

atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta  10451

agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc  10511

tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca  10571

tacactattc tcagaatgac ttggttg                                      10598

<210> 23
<211> 540
<212> PRT
<213> Artificial Sequence


<220> 
<223> [CDS]:4559..6181 from SEQ ID NO 22

<400> 23
Met Cys His Gln Gln Leu Val Ile Ser Trp Phe Ser Leu Val Phe Leu 
1               5                   10                  15      
Ala Ser Pro Leu Val Ala Ile Trp Glu Leu Lys Lys Asp Val Tyr Val 
            20                  25                  30          
Val Glu Leu Asp Trp Tyr Pro Asp Ala Pro Gly Glu Met Val Val Leu 
        35                  40                  45              
Thr Cys Asp Thr Pro Glu Glu Asp Gly Ile Thr Trp Thr Leu Asp Gln 
    50                  55                  60                  
Ser Ser Glu Val Leu Gly Ser Gly Lys Thr Leu Thr Ile Gln Val Lys 
65                  70                  75                  80  
Glu Phe Gly Asp Ala Gly Gln Tyr Thr Cys His Lys Gly Gly Glu Val 
                85                  90                  95      
Leu Ser His Ser Leu Leu Leu Leu His Lys Lys Glu Asp Gly Ile Trp 
            100                 105                 110         
Ser Thr Asp Ile Leu Lys Asp Gln Lys Glu Pro Lys Asn Lys Thr Phe 
        115                 120                 125             
Leu Arg Cys Glu Ala Lys Asn Tyr Ser Gly Arg Phe Thr Cys Trp Trp 
    130                 135                 140                 
Leu Thr Thr Ile Ser Thr Asp Leu Thr Phe Ser Val Lys Ser Ser Arg 
145                 150                 155                 160 
Gly Ser Ser Asp Pro Gln Gly Val Thr Cys Gly Ala Ala Thr Leu Ser 
                165                 170                 175     
Ala Glu Arg Val Arg Gly Asp Asn Lys Glu Tyr Glu Tyr Ser Val Glu 
            180                 185                 190         
Cys Gln Glu Asp Ser Ala Cys Pro Ala Ala Glu Glu Ser Leu Pro Ile 
        195                 200                 205             
Glu Val Met Val Asp Ala Val His Lys Leu Lys Tyr Glu Asn Tyr Thr 
    210                 215                 220                 
Ser Ser Phe Phe Ile Arg Asp Ile Ile Lys Pro Asp Pro Pro Lys Asn 
225                 230                 235                 240 
Leu Gln Leu Lys Pro Leu Lys Asn Ser Arg Gln Val Glu Val Ser Trp 
                245                 250                 255     
Glu Tyr Pro Asp Thr Trp Ser Thr Pro His Ser Tyr Phe Ser Leu Thr 
            260                 265                 270         
Phe Cys Val Gln Val Gln Gly Lys Ser Lys Arg Glu Lys Lys Asp Arg 
        275                 280                 285             
Val Phe Thr Asp Lys Thr Ser Ala Thr Val Ile Cys Arg Lys Asn Ala 
    290                 295                 300                 
Ser Ile Ser Val Arg Ala Gln Asp Arg Tyr Tyr Ser Ser Ser Trp Ser 
305                 310                 315                 320 
Glu Trp Ala Ser Val Pro Cys Ser Gly Gly Gly Gly Ser Gly Gly Gly 
                325                 330                 335     
Gly Ser Gly Gly Gly Gly Ser Arg Asn Leu Pro Val Ala Thr Pro Asp 
            340                 345                 350         
Pro Gly Met Phe Pro Cys Leu His His Ser Gln Asn Leu Leu Arg Ala 
        355                 360                 365             
Val Ser Asn Met Leu Gln Lys Ala Arg Gln Thr Leu Glu Phe Tyr Pro 
    370                 375                 380                 
Cys Thr Ser Glu Glu Ile Asp His Glu Asp Ile Thr Lys Asp Lys Thr 
385                 390                 395                 400 
Ser Thr Val Glu Ala Cys Leu Pro Leu Glu Leu Thr Lys Asn Glu Ser 
                405                 410                 415     
Cys Leu Asn Ser Arg Glu Thr Ser Phe Ile Thr Asn Gly Ser Cys Leu 
            420                 425                 430         
Ala Ser Arg Lys Thr Ser Phe Met Met Ala Leu Cys Leu Ser Ser Ile 
        435                 440                 445             
Tyr Glu Asp Leu Lys Met Tyr Gln Val Glu Phe Lys Thr Met Asn Ala 
    450                 455                 460                 
Lys Leu Leu Met Asp Pro Lys Arg Gln Ile Phe Leu Asp Gln Asn Met 
465                 470                 475                 480 
Leu Ala Val Ile Asp Glu Leu Met Gln Ala Leu Asn Phe Asn Ser Glu 
                485                 490                 495     
Thr Val Pro Gln Lys Ser Ser Leu Glu Glu Pro Asp Phe Tyr Lys Thr 
            500                 505                 510         
Lys Ile Lys Leu Cys Ile Leu Leu His Ala Phe Arg Ile Arg Ala Val 
        515                 520                 525             
Thr Ile Asp Arg Val Met Ser Tyr Leu Asn Ala Ser 
    530                 535                 540 

<210> 24
<211> 9476
<212> DNA
<213> Artificial Sequence


<220> 
<223> pCCL.PPT.NFκBenhsyn.EGFP.PGK.CAR.wPRE

<220> 
<221> misc_signal
<222> 2683..2808
<223> /note="HIV-1 psi packaging signal of human immunodeficiency virus
      type 1"

<220> 
<221> misc_feature
<222> 3301..3534
<223> /note="RRE: Rev response element (RRE) of HIV-1 allows for
      Rev-dependent mRNA export from the nucleus to the cytoplasm"

<220> 
<221> misc_feature
<222> 4030..4147
<223> /note="cPPT/CTS: central polypurine tract and central termination
      sequence of HIV-1"

<220> 
<221> promoter
<222> 4172..4275
<223> /function="NFκBenhsyn"

<220> 
<221> misc_feature
<222> 4284..4338
<223> /note="promoter section enhsyn"

<220> 
<221> CDS
<222> 4351..5070
<223> /gene="eGFP"
      /transl_table=1

<220> 
<221> gene
<222> 5626..7080
<223> /gene="CAR"

<220> 
<221> misc_feature
<222> 7107..7695
<223> /note="WPRE: woodchuck hepatitis virus posttranscriptional
      regulatory element"

<400> 24
agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca    60

gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag   120

gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc   180

gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg   240

tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc   300

ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg   360

cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg   420

gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga   480

cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac   540

tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa   600

aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca   660

aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag   720

gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac   780

cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa   840

ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc   900

accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag   960

tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac  1020

cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc  1080

gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc  1140

ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca  1200

cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc  1260

tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg  1320

ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct  1380

ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata  1440

ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc  1500

gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg  1560

acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca  1620

ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg  1680

tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa  1740

ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttggccattg catacgttgt  1800

atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac  1860

attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat  1920

atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg  1980

acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt  2040

tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag  2100

tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc  2160

attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag  2220

tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt  2280

ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc  2340

accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg  2400

gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccggggtc  2460

tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct  2520

taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga  2580

ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg  2640

cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact  2700

cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa  2760

attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg  2820

gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat  2880

aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc  2940

ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag  3000

acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat  3060

caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac  3120

aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat  3180

gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg  3240

agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat  3300

aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcctcaat  3360

gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt  3420

gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca  3480

gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat  3540

ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag  3600

taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat  3660

taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa  3720

gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat  3780

aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt  3840

aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt  3900

atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga  3960

agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta  4020

tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt  4080

agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca  4140

aaattttatc gatcacgaga ctagcctcga ggggaatttc cggggacttt ccgggaattt  4200

ccggggactt tccgggaatt tccgggaatt tccggggact ttccgggaat ttccggggac  4260

tttccgggaa tttccctgca ggagactcta gagggtatat aatggtttaa acttaagctt  4320

ggtaccgggc ccccgaagac gcgtgccacc atg gtg agc aag ggc gag gag ctg   4374
                                 Met Val Ser Lys Gly Glu Glu Leu 
                                 1               5               

ttc acc ggg gtg gtg ccc atc ctg gtc gag ctg gac ggc gac gta aac    4422
Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn 
    10                  15                  20                  

ggc cac aag ttc agc gtg tcc ggc gag ggc gag ggc gat gcc acc tac    4470
Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr 
25                  30                  35                  40  

ggc aag ctg acc ctg aag ttc atc tgc acc acc ggc aag ctg ccc gtg    4518
Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val 
                45                  50                  55      

ccc tgg ccc acc ctc gtg acc acc ctg acc tac ggc gtg cag tgc ttc    4566
Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val Gln Cys Phe 
            60                  65                  70          

agc cgc tac ccc gac cac atg aag cag cac gac ttc ttc aag tcc gcc    4614
Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala 
        75                  80                  85              

atg ccc gaa ggc tac gtc cag gag cgc acc atc ttc ttc aag gac gac    4662
Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp 
    90                  95                  100                 

ggc aac tac aag acc cgc gcc gag gtg aag ttc gag ggc gac acc ctg    4710
Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu 
105                 110                 115                 120 

gtg aac cgc atc gag ctg aag ggc atc gac ttc aag gag gac ggc aac    4758
Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn 
                125                 130                 135     

atc ctg ggg cac aag ctg gag tac aac tac aac agc cac aac gtc tat    4806
Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr 
            140                 145                 150         

atc atg gcc gac aag cag aag aac ggc atc aag gtg aac ttc aag atc    4854
Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile 
        155                 160                 165             

cgc cac aac atc gag gac ggc agc gtg cag ctc gcc gac cac tac cag    4902
Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln 
    170                 175                 180                 

cag aac acc ccc atc ggc gac ggc ccc gtg ctg ctg ccc gac aac cac    4950
Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His 
185                 190                 195                 200 

tac ctg agc acc cag tcc gcc ctg agc aaa gac ccc aac gag aag cgc    4998
Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg 
                205                 210                 215     

gat cac atg gtc ctg ctg gag ttc gtg acc gcc gcc ggg atc act ctc    5046
Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu 
            220                 225                 230         

ggc atg gac gag ctg tac aag taa tcgaattccc acggggttgg ggttgcgcct   5100
Gly Met Asp Glu Leu Tyr Lys                                      
        235                                                      

tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa  5160

acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg  5220

gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg  5280

ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac  5340

tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc  5400

tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt  5460

gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc  5520

attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga  5580

cctctctccc cagggggatc caccggtcac gtggctagcg ccaccnnnnn nnnnnnnnnn  5640

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5700

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5760

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5820

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5880

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5940

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6000

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6060

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6120

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6180

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6240

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6300

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6360

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6420

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6480

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6780

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6840

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6900

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6960

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  7020

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  7080

taggatccgc cggcggccgc gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat  7140

tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc  7200

ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct  7260

ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca  7320

ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt  7380

ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg  7440

cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga  7500

agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt  7560

ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc  7620

cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt  7680

gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga cttacaaggc  7740

agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc  7800

ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg  7860

agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc  7920

ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct  7980

cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat  8040

tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc  8100

agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt  8160

ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct  8220

ctagctatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta  8280

atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag  8340

tgaggaggct tttttggagg cctaggcttt tgcgtcgaga cgtacccaat tcgccctata  8400

gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc  8460

ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata  8520

gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc  8580

gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga  8640

ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg  8700

ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat  8760

ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg  8820

ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata  8880

gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt  8940

tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat  9000

ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc ccaggtggca cttttcgggg  9060

aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct  9120

catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat  9180

tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc  9240

tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg  9300

ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg  9360

ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga  9420

cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttg      9476

<210> 25
<211> 239
<212> PRT
<213> Artificial Sequence


<220> 
<223> [CDS]:4351..5070 from SEQ ID NO 24

<400> 25
Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      
Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          
Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              
Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  
Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  
Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      
Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         
Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             
Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 
Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 
Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     
Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         
Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 
Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 

<210> 26
<211> 7705
<212> DNA
<213> Artificial Sequence


<220> 
<223> pCAS.NFATenhsyn.EGFP.PGK.CAR.PRE SIN

<220> 
<221> promoter
<222> 446..1116
<223> /note="CMV promoter"

<220> 
<221> misc_feature
<222> 1117..1137
<223> /note="part of R element"

<220> 
<221> misc_feature
<222> 1138..1217
<223> /note="U5 element"

<220> 
<221> misc_feature
<222> 1218..1236
<223> /note="PBS"

<220> 
<221> promoter
<222> 1504..1747
<223> /note="promoter NFATenhsyn"

<220> 
<221> CDS
<222> 1760..2479
<223> /gene="eGFP"
      /transl_table=1

<220> 
<221> gene
<222> 3035..4489
<223> /note="CAR"

<220> 
<221> misc_feature
<222> 4732..5135
<223> /note="WPRE: woodchuck hepatitis virus posttranscriptional
      regulatory element"

<220> 
<221> misc_feature
<222> 5220..5273
<223> /note="dU3 element"

<220> 
<221> misc_feature
<222> 5274..5294
<223> /note="part of R element"

<400> 26
ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac    60

ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc   120

gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gttactatgc   180

ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg   240

cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga   300

agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc   360

aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc   420

cagtgaatta gtactctagc ttaagccatt gcatacgttg tatccatatc ataatatgta   480

catttatatt ggctcatgtc caacattacc gccatgttga cattgattat tgactagtta   540

ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac   600

ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc   660

aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt   720

ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac   780

gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac   840

cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt   900

gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc   960

aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt  1020

tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg  1080

ggaggtctat ataagcagag ctcgtttagt gaaccggcca tttgaccatt caccacattg  1140

gtgtgcacct gggttgatgg ccggaccgtt gattccctga cgactacgag cacctgcatg  1200

aagcagaagg cttcatttgg tgaccccgac gtgatagtta gggaatagtg gtcggccaca  1260

gacggcgtgg cgatcctgtc tccatccgtc tcgtctatcg ggaggcgagt tcgatgaccc  1320

tggtggaggg ggctgcggct tagggaggca gaagctgagt accgtcggag ggagctccag  1380

ggcccggagc gactgacccc tgccgagaac tcagagggtc gtcggaagac ggagagtgag  1440

cccgacgacc accccaggca cgtctttggt cggcctgcgg atcaagcagg gcccgctcga  1500

gcgtggagga aaaactgttt catacagaag gcgtggagga aaaactgttt catacagaag  1560

gcgtggagga aaaactgttt catacagaag gcgtggagga aaaactgttt catacagaag  1620

gcgtggagga aaaactgttt catacagaag gcgtggagga aaaactgttt catacagaag  1680

gcgtctgcag gagactctag agggtatata atggtttaaa cttaagcttg gtaccgggcc  1740

cccgaagacg cgtgccacc atg gtg agc aag ggc gag gag ctg ttc acc ggg   1792
                     Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly 
                     1               5                   10      

gtg gtg ccc atc ctg gtc gag ctg gac ggc gac gta aac ggc cac aag    1840
Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys 
            15                  20                  25          

ttc agc gtg tcc ggc gag ggc gag ggc gat gcc acc tac ggc aag ctg    1888
Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu 
        30                  35                  40              

acc ctg aag ttc atc tgc acc acc ggc aag ctg ccc gtg ccc tgg ccc    1936
Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro 
    45                  50                  55                  

acc ctc gtg acc acc ctg acc tac ggc gtg cag tgc ttc agc cgc tac    1984
Thr Leu Val Thr Thr Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr 
60                  65                  70                  75  

ccc gac cac atg aag cag cac gac ttc ttc aag tcc gcc atg ccc gaa    2032
Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu 
                80                  85                  90      

ggc tac gtc cag gag cgc acc atc ttc ttc aag gac gac ggc aac tac    2080
Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr 
            95                  100                 105         

aag acc cgc gcc gag gtg aag ttc gag ggc gac acc ctg gtg aac cgc    2128
Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg 
        110                 115                 120             

atc gag ctg aag ggc atc gac ttc aag gag gac ggc aac atc ctg ggg    2176
Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly 
    125                 130                 135                 

cac aag ctg gag tac aac tac aac agc cac aac gtc tat atc atg gcc    2224
His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala 
140                 145                 150                 155 

gac aag cag aag aac ggc atc aag gtg aac ttc aag atc cgc cac aac    2272
Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn 
                160                 165                 170     

atc gag gac ggc agc gtg cag ctc gcc gac cac tac cag cag aac acc    2320
Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr 
            175                 180                 185         

ccc atc ggc gac ggc ccc gtg ctg ctg ccc gac aac cac tac ctg agc    2368
Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser 
        190                 195                 200             

acc cag tcc gcc ctg agc aaa gac ccc aac gag aag cgc gat cac atg    2416
Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met 
    205                 210                 215                 

gtc ctg ctg gag ttc gtg acc gcc gcc ggg atc act ctc ggc atg gac    2464
Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp 
220                 225                 230                 235 

gag ctg tac aag taa tcgaattccc acggggttgg ggttgcgcct tttccaaggc    2519
Glu Leu Tyr Lys                                                 
                                                                

agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc  2579

gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc  2639

gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc  2699

ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc  2759

gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat  2819

agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg  2879

gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag  2939

cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc  2999

cagggggatc caccggtcac gtggctagcg ccaccnnnnn nnnnnnnnnn nnnnnnnnnn  3059

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3119

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3179

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3239

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3299

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3359

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3419

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3479

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3539

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3599

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3659

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3719

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3779

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3839

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3899

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3959

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  4019

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  4079

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  4139

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  4199

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  4259

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  4319

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  4379

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  4439

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn taggatccgc  4499

cggcggccgc gtcgacggat cccccgggct gcaggaattc gagcatctta ccgccattta  4559

tacccatatt tgttctgttt ttcttgattt gggtatacat ttaaatgtta ataaaacaaa  4619

atggtggggc aatcatttac atttttaggg atatgtaatt actagttcag gtgtattgcc  4679

acaagacaaa catgttaaga aactttcccg ttatttacgc tctgttcctg ttaatcaacc  4739

tctggattac aaaatttgtg aaagattgac tgatattctt aactatgttg ctccttttac  4799

gctgtgtgga tatgctgctt taatgcctct gtatcatgct attgcttccc gtacggcttt  4859

cgttttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt  4919

tgtccgtcaa cgtggcgtgg tgtgctctgt gtttgctgac gcaaccccca ctggctgggg  4979

cattgccacc acctgtcaac tcctttctgg gactttcgct ttccccctcc cgatcgccac  5039

ggcagaactc atcgccgcct gccttgcccg ctgctggaca ggggctaggt tgctgggcac  5099

tgataattcc gtggtgttgt cggggaagct gacgtccttt cgaattcgaa agcttttaaa  5159

tatcgatgcg atgtacgggc cagatatacg cgtatctgag gggactaggg tgtgtttagg  5219

cgaaaagcgg ggcttcggtt gtacgcggtt aggagtcccc ttaggatata gtagtttcgc  5279

ttttgcatag ggagggggaa atgtagtctt atgcaatact cttgtacgta gtgcctagct  5339

cgatacaata aacgccattt gaccattcac cacattggtg tgcacctggg ttgatggccg  5399

gaccgttgat tccctgacga ctacgagcac ctgcatgaag cagaaggctt cattctcgag  5459

agctttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat  5519

tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag  5579

ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg  5639

ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc  5699

ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc  5759

agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa  5819

catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt  5879

tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg  5939

gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg  5999

ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag  6059

cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc  6119

caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa  6179

ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg  6239

taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc  6299

taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac  6359

cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg  6419

tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt  6479

gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt  6539

catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa  6599

atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga  6659

ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt  6719

gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg  6779

agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga  6839

gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga  6899

agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgctgg  6959

catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc  7019

aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc  7079

gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca  7139

taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac  7199

caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg  7259

ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc  7319

ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg  7379

tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac  7439

aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat  7499

actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata  7559

catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa  7619

agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg  7679

tatcacgagg ccctttcgtc ttcaag                                       7705

<210> 27
<211> 239
<212> PRT
<213> Artificial Sequence


<220> 
<223> [CDS]:1760..2479 from SEQ ID NO 26

<400> 27
Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      
Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          
Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              
Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  
Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  
Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      
Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         
Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             
Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 
Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 
Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     
Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         
Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 
Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 

<210> 28
<211> 7626
<212> DNA
<213> Artificial Sequence


<220> 
<223> pCAS.NFκBenhsyn.EGFP.PGK.CAR.PRE SIN

<220> 
<221> promoter
<222> 1496..1668
<223> /note="promoter NFκBenhsyn"

<220> 
<221> CDS
<222> 1681..2400
<223> /gene="eGFP"
      /transl_table=1

<220> 
<221> gene
<222> 2956..4410
<223> /gene="CAR"

<220> 
<221> misc_feature
<222> 4460..5062
<223> /note="PRE"

<220> 
<221> misc_feature
<222> 4653..5056
<223> /note="WPRE: woodchuck hepatitis virus posttranscriptional
      regulatory element"

<400> 28
ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac    60

ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc   120

gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gttactatgc   180

ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg   240

cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga   300

agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc   360

aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc   420

cagtgaatta gtactctagc ttaagccatt gcatacgttg tatccatatc ataatatgta   480

catttatatt ggctcatgtc caacattacc gccatgttga cattgattat tgactagtta   540

ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac   600

ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc   660

aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt   720

ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac   780

gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac   840

cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt   900

gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc   960

aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt  1020

tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg  1080

ggaggtctat ataagcagag ctcgtttagt gaaccggcca tttgaccatt caccacattg  1140

gtgtgcacct gggttgatgg ccggaccgtt gattccctga cgactacgag cacctgcatg  1200

aagcagaagg cttcatttgg tgaccccgac gtgatagtta gggaatagtg gtcggccaca  1260

gacggcgtgg cgatcctgtc tccatccgtc tcgtctatcg ggaggcgagt tcgatgaccc  1320

tggtggaggg ggctgcggct tagggaggca gaagctgagt accgtcggag ggagctccag  1380

ggcccggagc gactgacccc tgccgagaac tcagagggtc gtcggaagac ggagagtgag  1440

cccgacgacc accccaggca cgtctttggt cggcctgcgg atcaagcagg gcccgctcga  1500

ggggaatttc cggggacttt ccgggaattt ccggggactt tccgggaatt tccgggaatt  1560

tccggggact ttccgggaat ttccggggac tttccgggaa tttccctgca ggagactcta  1620

gagggtatat aatggtttaa acttaagctt ggtaccgggc ccccgaagac gcgtgccacc  1680

atg gtg agc aag ggc gag gag ctg ttc acc ggg gtg gtg ccc atc ctg    1728
Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      

gtc gag ctg gac ggc gac gta aac ggc cac aag ttc agc gtg tcc ggc    1776
Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          

gag ggc gag ggc gat gcc acc tac ggc aag ctg acc ctg aag ttc atc    1824
Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              

tgc acc acc ggc aag ctg ccc gtg ccc tgg ccc acc ctc gtg acc acc    1872
Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  

ctg acc tac ggc gtg cag tgc ttc agc cgc tac ccc gac cac atg aag    1920
Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  

cag cac gac ttc ttc aag tcc gcc atg ccc gaa ggc tac gtc cag gag    1968
Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      

cgc acc atc ttc ttc aag gac gac ggc aac tac aag acc cgc gcc gag    2016
Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         

gtg aag ttc gag ggc gac acc ctg gtg aac cgc atc gag ctg aag ggc    2064
Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             

atc gac ttc aag gag gac ggc aac atc ctg ggg cac aag ctg gag tac    2112
Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 

aac tac aac agc cac aac gtc tat atc atg gcc gac aag cag aag aac    2160
Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 

ggc atc aag gtg aac ttc aag atc cgc cac aac atc gag gac ggc agc    2208
Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     

gtg cag ctc gcc gac cac tac cag cag aac acc ccc atc ggc gac ggc    2256
Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         

ccc gtg ctg ctg ccc gac aac cac tac ctg agc acc cag tcc gcc ctg    2304
Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             

agc aaa gac ccc aac gag aag cgc gat cac atg gtc ctg ctg gag ttc    2352
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 

gtg acc gcc gcc ggg atc act ctc ggc atg gac gag ctg tac aag taa    2400
Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys     
225                 230                 235                     

tcgaattccc acggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg  2460

acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac  2520

attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc  2580

ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg  2640

gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg  2700

agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcggggcg  2760

cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg  2820

ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc  2880

agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggatc caccggtcac  2940

gtggctagcg ccaccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3000

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3060

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3120

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3180

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3240

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3300

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3360

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3420

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3480

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3780

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3840

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3900

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  3960

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  4020

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  4080

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  4140

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  4200

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  4260

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  4320

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  4380

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn taggatccgc cggcggccgc gtcgacggat  4440

cccccgggct gcaggaattc gagcatctta ccgccattta tacccatatt tgttctgttt  4500

ttcttgattt gggtatacat ttaaatgtta ataaaacaaa atggtggggc aatcatttac  4560

atttttaggg atatgtaatt actagttcag gtgtattgcc acaagacaaa catgttaaga  4620

aactttcccg ttatttacgc tctgttcctg ttaatcaacc tctggattac aaaatttgtg  4680

aaagattgac tgatattctt aactatgttg ctccttttac gctgtgtgga tatgctgctt  4740

taatgcctct gtatcatgct attgcttccc gtacggcttt cgttttctcc tccttgtata  4800

aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtccgtcaa cgtggcgtgg  4860

tgtgctctgt gtttgctgac gcaaccccca ctggctgggg cattgccacc acctgtcaac  4920

tcctttctgg gactttcgct ttccccctcc cgatcgccac ggcagaactc atcgccgcct  4980

gccttgcccg ctgctggaca ggggctaggt tgctgggcac tgataattcc gtggtgttgt  5040

cggggaagct gacgtccttt cgaattcgaa agcttttaaa tatcgatgcg atgtacgggc  5100

cagatatacg cgtatctgag gggactaggg tgtgtttagg cgaaaagcgg ggcttcggtt  5160

gtacgcggtt aggagtcccc ttaggatata gtagtttcgc ttttgcatag ggagggggaa  5220

atgtagtctt atgcaatact cttgtacgta gtgcctagct cgatacaata aacgccattt  5280

gaccattcac cacattggtg tgcacctggg ttgatggccg gaccgttgat tccctgacga  5340

ctacgagcac ctgcatgaag cagaaggctt cattctcgag agctttggcg taatcatggt  5400

catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg  5460

gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt  5520

tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg  5580

gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg  5640

actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa  5700

tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc  5760

aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc  5820

ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat  5880

aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc  5940

cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct  6000

cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg  6060

aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc  6120

cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga  6180

ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa  6240

ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta  6300

gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc  6360

agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg  6420

acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga  6480

tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg  6540

agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct  6600

gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg  6660

agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc  6720

cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa  6780

ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc  6840

cagttaatag tttgcgcaac gttgttgcca ttgctgctgg catcgtggtg tcacgctcgt  6900

cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc  6960

ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt  7020

tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc  7080

catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt  7140

gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata  7200

gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga  7260

tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag  7320

catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa  7380

aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt  7440

attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga  7500

aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag  7560

aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc  7620

ttcaag                                                             7626

<210> 29
<211> 239
<212> PRT
<213> Artificial Sequence


<220> 
<223> [CDS]:1681..2400 from SEQ ID NO 28

<400> 29
Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      
Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          
Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              
Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  
Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  
Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      
Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         
Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             
Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 
Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 
Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     
Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         
Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 
Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 

<210> 30
<211> 239
<212> PRT
<213> Artificial Sequence


<220> 
<223> [CDS]:4430..5149 from SEQ ID NO 8

<400> 30
Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      
Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          
Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              
Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  
Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  
Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      
Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         
Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             
Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 
Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 
Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     
Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         
Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 
Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 

<210> 31
<211> 224
<212> DNA
<213> Artificial Sequence


<220> 
<223> NFκBmIL2 promoter

<220> 
<221> TATA_signal
<222> 151..157
<223> /note="TATA box"

<400> 31
gggaatttcc ggggactttc cgggaatttc cggggacttt ccgggaattt ccgggaattt     60

ccggggactt tccgggaatt tccggggact ttccgggaat ttcccccggg acattttgac    120

acccccataa tatttttcca gaattaacag tataaattgc atctcttgtt caagagttcc    180

ctatcactct ctttaatcac tactcacagt aacctcaact cctg                     224


<210> 32
<211> 9544
<212> DNA
<213> Artificial Sequence


<220> 
<223> pCCL.PPT.NFκBmIL2.EGFP.PGK.CAR.WPRE

<220> 
<221> misc_signal
<222> 2683..2808
<223> /note="HIV-1 psi packaging signal of human immunodeficiency virus
      type 1"

<220> 
<221> misc_feature
<222> 3301..3535
<223> /note="RRE - Rev response element of HIV-1 allows for
      Rev-dependent mRNA export from the nucleus to the cytoplasm"

<220> 
<221> misc_feature
<222> 4030..4147
<223> /note="cPPT/CTS: central polypurine tract and central termination
      sequence of HIV-1"

<220> 
<221> promoter
<222> 4178..4281
<223> /note="NFκB"

<220> 
<221> promoter
<222> 4178..4400
<223> /note="NFκBmIL2"

<220> 
<221> misc_feature
<222> 4282..4400
<223> /note="mIL2"

<220> 
<221> CDS
<222> 4419..5138
<223> /gene="eGFP"
      /transl_table=1

<220> 
<221> misc_feature
<222> 5694..7148
<223> /note="CAR"

<220> 
<221> misc_feature
<222> 7163..7768
<223> /note="PRE"

<220> 
<221> misc_feature
<222> 7175..7763
<223> /note="WPRE: woodchuck hepatitis virus posttranscriptional
      regulatory element"

<400> 32
agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca    60

gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag   120

gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc   180

gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg   240

tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc   300

ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg   360

cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg   420

gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga   480

cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac   540

tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa   600

aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca   660

aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag   720

gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac   780

cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa   840

ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc   900

accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag   960

tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac  1020

cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc  1080

gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc  1140

ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca  1200

cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc  1260

tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg  1320

ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct  1380

ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata  1440

ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc  1500

gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg  1560

acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca  1620

ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg  1680

tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa  1740

ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttggccattg catacgttgt  1800

atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac  1860

attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat  1920

atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg  1980

acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt  2040

tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag  2100

tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc  2160

attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag  2220

tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt  2280

ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc  2340

accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg  2400

gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccggggtc  2460

tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct  2520

taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga  2580

ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg  2640

cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact  2700

cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa  2760

attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg  2820

gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat  2880

aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc  2940

ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag  3000

acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat  3060

caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac  3120

aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat  3180

gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg  3240

agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat  3300

aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcctcaat  3360

gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt  3420

gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca  3480

gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat  3540

ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag  3600

taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat  3660

taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa  3720

gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat  3780

aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt  3840

aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt  3900

atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga  3960

agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta  4020

tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt  4080

agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca  4140

aaattttatc gatcacgaga ctagcctcga cagcgctggg aatttccggg gactttccgg  4200

gaatttccgg ggactttccg ggaatttccg ggaatttccg gggactttcc gggaatttcc  4260

ggggactttc cgggaatttc ccccgggaca ttttgacacc cccataatat ttttccagaa  4320

ttaacagtat aaattgcatc tcttgttcaa gagttcccta tcactctctt taatcactac  4380

tcacagtaac ctcaactcct ggatccacgc gtgccacc atg gtg agc aag ggc gag  4436
                                          Met Val Ser Lys Gly Glu 
                                          1               5       

gag ctg ttc acc ggg gtg gtg ccc atc ctg gtc gag ctg gac ggc gac    4484
Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp 
            10                  15                  20          

gta aac ggc cac aag ttc agc gtg tcc ggc gag ggc gag ggc gat gcc    4532
Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala 
        25                  30                  35              

acc tac ggc aag ctg acc ctg aag ttc atc tgc acc acc ggc aag ctg    4580
Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu 
    40                  45                  50                  

ccc gtg ccc tgg ccc acc ctc gtg acc acc ctg acc tac ggc gtg cag    4628
Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val Gln 
55                  60                  65                  70  

tgc ttc agc cgc tac ccc gac cac atg aag cag cac gac ttc ttc aag    4676
Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys 
                75                  80                  85      

tcc gcc atg ccc gaa ggc tac gtc cag gag cgc acc atc ttc ttc aag    4724
Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys 
            90                  95                  100         

gac gac ggc aac tac aag acc cgc gcc gag gtg aag ttc gag ggc gac    4772
Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp 
        105                 110                 115             

acc ctg gtg aac cgc atc gag ctg aag ggc atc gac ttc aag gag gac    4820
Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp 
    120                 125                 130                 

ggc aac atc ctg ggg cac aag ctg gag tac aac tac aac agc cac aac    4868
Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn 
135                 140                 145                 150 

gtc tat atc atg gcc gac aag cag aag aac ggc atc aag gtg aac ttc    4916
Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe 
                155                 160                 165     

aag atc cgc cac aac atc gag gac ggc agc gtg cag ctc gcc gac cac    4964
Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His 
            170                 175                 180         

tac cag cag aac acc ccc atc ggc gac ggc ccc gtg ctg ctg ccc gac    5012
Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp 
        185                 190                 195             

aac cac tac ctg agc acc cag tcc gcc ctg agc aaa gac ccc aac gag    5060
Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu 
    200                 205                 210                 

aag cgc gat cac atg gtc ctg ctg gag ttc gtg acc gcc gcc ggg atc    5108
Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile 
215                 220                 225                 230 

act ctc ggc atg gac gag ctg tac aag taa tcgaattccc acggggttgg      5158
Thr Leu Gly Met Asp Glu Leu Tyr Lys                           
                235                                           

ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg  5218

gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca  5278

gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc  5338

ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg  5398

cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac  5458

cgcgatgggc tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg  5518

aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc  5578

ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc  5638

gaatcaccga cctctctccc cagggggatc caccggtcac gtggctagcg ccaccnnnnn  5698

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5758

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5818

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5878

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5938

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  5998

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6058

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6118

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6178

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6238

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6298

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6358

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6418

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6478

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6538

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6598

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6658

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6718

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6778

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6838

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6898

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  6958

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  7018

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  7078

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn  7138

nnnnnnnnnn taggatccgc cggcggccgc gtcgacaatc aacctctgga ttacaaaatt  7198

tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct  7258

gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg  7318

tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc  7378

gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt  7438

cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc  7498

gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg  7558

ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg  7618

cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc  7678

ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg  7738

atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga  7798

cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc  7858

taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag  7918

accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat  7978

aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact  8038

agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc  8098

atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact  8158

tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata  8218

aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc  8278

atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca  8338

tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt  8398

ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcgtcgaga cgtacccaat  8458

tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac  8518

tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc  8578

tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat  8638

ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg  8698

cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct  8758

tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta  8818

gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt  8878

tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg  8938

ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat  8998

tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt  9058

taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc ccaggtggca  9118

cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata  9178

tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga  9238

gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc  9298

ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg  9358

cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc  9418

ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat  9478

cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact  9538

tggttg                                                             9544

<210> 33
<211> 239
<212> PRT
<213> Artificial Sequence


<220> 
<223> [CDS]:4419..5138 from SEQ ID NO 32

<400> 33
Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      
Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          
Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              
Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  
Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  
Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      
Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         
Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             
Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 
Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 
Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     
Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         
Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 
Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 

<210> 34
<211> 9314
<212> DNA
<213> Artificial Sequence


<220> 
<223> pCCL.PPT.NFATenhsyn.humanIL21co.PGK.CAR.PRE

<220> 
<221> enhancer
<222> 1858..2237
<223> /note="CMV enhancer"

<220> 
<221> promoter
<222> 2238..2441
<223> /note="CMV promoter"

<220> 
<221> LTR
<222> 2456..2636
<223> /note="5´LTR"

<220> 
<221> misc_signal
<222> 2683..2808
<223> /note="HIV-1 psi packaging signal"

<220> 
<221> misc_signal
<222> 3301..3534
<223> /note="RRE rev response element fo HIV-1, allows fo Rev-dependent
      mRNA export for nucleus to cytoplasm"

<220> 
<221> misc_feature
<222> 4030..4147
<223> /note="cPPT/CTS central purine tract and central termination
      sequence of HIV-1"

<220> 
<221> promoter
<222> 4174..4417
<223> /note="NFATenhsyn"

<220> 
<221> gene
<222> 4430..4916
<223> /gene="human IL21, codon-optimized"

<220> 
<221> promoter
<222> 4921..5431
<223> /note="hPGK promoter"

<220> 
<221> gene
<222> 5464..6918
<223> /note="placeholder for CAR"

<220> 
<221> misc_signal
<222> 6945..7533
<223> /note="WPRE"

<220> 
<221> LTR
<222> 7620..8046
<223> /note="3´LTR (delta U3), self-inactivating 3´ long terminal
      repeat (LTR) of HIV-1"

<400> 34
agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca       60

gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag      120

gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc      180

gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg      240

tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc      300

ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg      360

cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg      420

gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga      480

cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac      540

tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa      600

aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca      660

aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag      720

gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac      780

cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa      840

ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc      900

accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag      960

tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac     1020

cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc     1080

gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc     1140

ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca     1200

cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc     1260

tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg     1320

ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct     1380

ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata     1440

ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc     1500

gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg     1560

acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca     1620

ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg     1680

tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa     1740

ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttggccattg catacgttgt     1800

atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac     1860

attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat     1920

atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg     1980

acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt     2040

tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag     2100

tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc     2160

attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag     2220

tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt     2280

ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc     2340

accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg     2400

gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccggggtc     2460

tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct     2520

taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga     2580

ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg     2640

cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact     2700

cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa     2760

attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg     2820

gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat     2880

aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc     2940

ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag     3000

acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat     3060

caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac     3120

aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat     3180

gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg     3240

agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat     3300

aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcctcaat     3360

gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt     3420

gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca     3480

gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat     3540

ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag     3600

taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat     3660

taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa     3720

gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat     3780

aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt     3840

aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt     3900

atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga     3960

agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta     4020

tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt     4080

agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca     4140

aaattttatc gatcacgaga ctagcctcga gcgtggagga aaaactgttt catacagaag     4200

gcgtggagga aaaactgttt catacagaag gcgtggagga aaaactgttt catacagaag     4260

gcgtggagga aaaactgttt catacagaag gcgtggagga aaaactgttt catacagaag     4320

gcgtggagga aaaactgttt catacagaag gcgtctgcag gagactctag agggtatata     4380

atggtttaaa cttaagcttg gtaccgggcc cccgaagacg cgtgccacca tggaacggat     4440

cgtgatctgc ctgatggtca tcttcctggg caccctggtg cacaagagca gctctcaggg     4500

ccaagaccgg cacatgatcc ggatgagaca gctgatcgac atcgtggacc agctgaagaa     4560

ctacgtgaac gacctggtgc ctgagttcct gcctgctcct gaggacgtgg aaacaaactg     4620

cgagtggagc gccttcagct gcttccagaa ggcccagctg aaaagcgcca acaccggcaa     4680

caacgagcgg atcatcaacg tgtccatcaa gaagctgaag cggaagcctc ctagcaccaa     4740

cgccggaaga aggcagaagc acagactgac ctgtcctagc tgcgacagct acgagaagaa     4800

gcctccaaaa gagttcctgg aacggttcaa gagcctgctg cagaagatga tccaccagca     4860

cctgagcagc agaacccacg gcagcgagga ttcttgatgt acaagtaatc gaattcccac     4920

ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc     4980

tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc     5040

cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc     5100

tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac     5160

ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc     5220

gcgccgaccg cgatgggctg tggccaatag cggctgctca gcggggcgcg ccgagagcag     5280

cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct     5340

gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct     5400

cgttgaccga atcaccgacc tctctcccca gggggatcca ccggtcacgt ggctagcgcc     5460

accnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     5520

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     5580

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     5640

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     5700

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     5760

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     5820

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     5880

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     5940

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6000

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6060

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6120

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6180

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6240

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6300

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6360

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6420

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6480

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6780

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6840

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6900

nnnnnnnnnn nnnnnnnnta ggatccgccg gcggccgcgt cgacaatcaa cctctggatt     6960

acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg     7020

gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct     7080

cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc     7140

aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca     7200

ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac     7260

tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt     7320

ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct     7380

ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc     7440

cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga     7500

cgagtcggat ctccctttgg gccgcctccc cgcctggaat tcgagctcgg tacctttaag     7560

accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact     7620

ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct     7680

ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa     7740

gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc     7800

tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag     7860

ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga     7920

gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt     7980

cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt     8040

atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct     8100

ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct     8160

gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg cgtcgagacg     8220

tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac     8280

gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt     8340

tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca     8400

gcctgaatgg cgaatggcgc gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg     8460

tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt     8520

tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc     8580

tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg     8640

gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg     8700

agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct     8760

cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg     8820

agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttccc     8880

aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca     8940

ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa     9000

aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt     9060

ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca     9120

gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag     9180

ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc     9240

ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca     9300

gaatgacttg gttg                                                       9314


<210> 35
<211> 9692
<212> DNA
<213> Artificial Sequence


<220> 
<223> pCCL.PPT.NFATenhsyn.TRAIL.PGK.CAR.PRE

<220> 
<221> enhancer
<222> 1858..2237
<223> /note="CMV enhancer"

<220> 
<221> promoter
<222> 2238..2441
<223> /note="CMV promoter"

<220> 
<221> LTR
<222> 2456..2636
<223> /note="5´LTR, truncated"

<220> 
<221> misc_signal
<222> 2683..2808
<223> /note="psi packaging signal of HIV-1"

<220> 
<221> misc_signal
<222> 3301..3534
<223> /note="RRE of HIV-1"

<220> 
<221> misc_signal
<222> 4030..4147
<223> /note="cPPT/CTS"

<220> 
<221> promoter
<222> 4174..4417
<223> /note="NFATenhsyn"

<220> 
<221> gene
<222> 4430..5275
<223> /note="TRAIL"

<220> 
<221> promoter
<222> 5299..5809
<223> /note="hPGK promoter"

<220> 
<221> gene
<222> 5842..7296
<223> /note="placeholder for CAR"

<220> 
<221> misc_signal
<222> 7323..7911
<223> /note="WPRE"

<220> 
<221> LTR
<222> 7998..8231
<223> /note="3´LTR (delta U3)"

<400> 35
agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca       60

gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag      120

gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc      180

gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg      240

tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc      300

ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg      360

cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg      420

gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga      480

cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac      540

tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa      600

aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca      660

aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag      720

gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac      780

cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa      840

ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc      900

accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag      960

tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac     1020

cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc     1080

gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc     1140

ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca     1200

cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc     1260

tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg     1320

ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct     1380

ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata     1440

ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc     1500

gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg     1560

acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca     1620

ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg     1680

tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa     1740

ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttggccattg catacgttgt     1800

atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac     1860

attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat     1920

atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg     1980

acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt     2040

tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag     2100

tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc     2160

attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag     2220

tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt     2280

ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc     2340

accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg     2400

gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccggggtc     2460

tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct     2520

taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga     2580

ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg     2640

cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact     2700

cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa     2760

attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg     2820

gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat     2880

aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc     2940

ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag     3000

acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat     3060

caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac     3120

aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat     3180

gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg     3240

agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat     3300

aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcctcaat     3360

gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt     3420

gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca     3480

gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat     3540

ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag     3600

taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat     3660

taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa     3720

gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat     3780

aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt     3840

aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt     3900

atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga     3960

agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta     4020

tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt     4080

agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca     4140

aaattttatc gatcacgaga ctagcctcga gcgtggagga aaaactgttt catacagaag     4200

gcgtggagga aaaactgttt catacagaag gcgtggagga aaaactgttt catacagaag     4260

gcgtggagga aaaactgttt catacagaag gcgtggagga aaaactgttt catacagaag     4320

gcgtggagga aaaactgttt catacagaag gcgtctgcag gagactctag agggtatata     4380

atggtttaaa cttaagcttg gtaccgggcc cccgaagacg cgtgccacca tggctatgat     4440

ggaagtgcaa ggcggaccta gcctgggcca gacatgtgtg ctgatcgtga tcttcaccgt     4500

gctgctgcag agcctgtgtg tggccgtgac ctacgtgtac ttcaccaacg agctgaagca     4560

gatgcaggac aagtacagca agagcggaat cgcctgcttc ctgaaagagg acgacagcta     4620

ctgggacccc aacgacgaag agagcatgaa cagcccctgc tggcaagtga agtggcagct     4680

gagacagctc gtgcggaaga tgatcctgcg gaccagcgaa gagacaatca gcaccgtgca     4740

agagaagcag cagaacatca gccctctcgt cagagagcgg ggacctcaga gagtggccgc     4800

tcacattact ggcaccagag gcagaagcaa caccctgagc agccccaaca gcaagaacga     4860

gaaggccctg ggcagaaaga tcaacagctg ggagagcagc agatccggcc acagctttct     4920

gagcaacctg cacctgagaa acggcgagct ggtcatccac gagaagggct tctactacat     4980

ctacagccag acctacttcc ggttccaaga ggaaatcaaa gagaatacca agaacgacaa     5040

gcagatggtg cagtacatct ataagtacac gagctacccc gatccgatcc tgctgatgaa     5100

gtccgccaga aacagctgct ggtccaagga tgccgagtac ggcctgtata gcatctacca     5160

aggcggcatc ttcgagctga aagagaatga ccggatcttc gtgtccgtga ccaatgagca     5220

cctgatcgac atggaccacg aggccagctt tttcggcgcc tttcttgtgg gctgatgtac     5280

aagtaatcga attcccacgg ggttggggtt gcgccttttc caaggcagcc ctgggtttgc     5340

gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc     5400

tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg     5460

ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc     5520

gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg     5580

ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc     5640

ggggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt     5700

agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac     5760

gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg gggatccacc     5820

ggtcacgtgg ctagcgccac cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     5880

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     5940

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6000

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6060

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6120

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6180

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6240

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6300

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6360

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6420

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6480

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6780

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6840

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6900

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     6960

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     7020

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     7080

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     7140

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     7200

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn     7260

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntagg atccgccggc ggccgcgtcg     7320

acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg     7380

ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc     7440

gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt     7500

tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca     7560

ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc     7620

ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc     7680

tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc     7740

tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc     7800

tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc     7860

ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc     7920

gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt     7980

aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt     8040

tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact     8100

agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc     8160

ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa     8220

aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa     8280

atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag     8340

caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt     8400

gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg     8460

cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc     8520

gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta     8580

ggcttttgcg tcgagacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac     8640

tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc     8700

ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc     8760

cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga cgcgccctgt agcggcgcat     8820

taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag     8880

cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc     8940

aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc     9000

ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt     9060

ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa     9120

caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg     9180

cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat     9240

taacgtttac aatttcccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg     9300

tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat     9360

gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat     9420

tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt     9480

aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag     9540

cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa     9600

agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg     9660

ccgcatacac tattctcaga atgacttggt tg                                   9692


