               SEQUENCE LISTING

<110> Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V.
      Universitaet Heidelberg

<120> Site-specific, kinetically inert conjugation of labels and/or carriers to target molecules such as His-tagged proteins via metal complex reagents

<130> AC1351 PCT S3

<150> EP19 18 0999.5
<151> 2019-06-18

<160> 16

<170> BiSSAP 1.3.6

<210> 1
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> Consensus sequence chelating peptide

<400> 1
Gly His His Pro His Gly 
1               5       

<210> 2
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> Consensus sequence chelating peptide

<400> 2
Gly His His Pro His Gly His His Pro His Gly 
1               5                   10      

<210> 3
<211> 16
<212> PRT
<213> Artificial Sequence


<220> 
<223> Consensus sequence chelating peptide

<400> 3
Gly His His Pro His Gly His His Pro His Gly His His Pro His Gly 
1               5                   10                  15      



<210> 4
<211> 20
<212> PRT
<213> Artificial Sequence


<220> 
<223> Metal cation ligand amino acid motif k=2

<220> 
<221> VARIANT
<222> 1
<223> X = G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R, U, O

<220> 
<221> VARIANT
<222> 2..4
<223> X = is absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N,
      R, U, O

<220> 
<221> VARIANT
<222> 5..10
<223> X = is absent or L, I, V

<220> 
<221> VARIANT
<222> 11
<223> X = G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R, U, O

<220> 
<221> VARIANT
<222> 12..14
<223> X= absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      U, O

<220> 
<221> VARIANT
<222> 15..20
<223> X = absent or L, I, V

<400> 4
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      
Xaa Xaa Xaa Xaa 
            20  

<210> 5
<211> 30
<212> PRT
<213> Artificial Sequence


<220> 
<223> Metal cation ligand amino acid motif k=3

<220> 
<221> VARIANT
<222> 1
<223> X = G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R, U, O

<220> 
<221> VARIANT
<222> 2..4
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      U, O

<220> 
<221> VARIANT
<222> 5..10
<223> X = absent or L, I, V

<220> 
<221> VARIANT
<222> 11
<223> X = G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R, U, O

<220> 
<221> VARIANT
<222> 12..14
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      U, O

<220> 
<221> VARIANT
<222> 15..20
<223> X = absent or L, I, V

<220> 
<221> VARIANT
<222> 21
<223> X = G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R, U, O

<220> 
<221> VARIANT
<222> 22..24
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      U, O

<220> 
<221> VARIANT
<222> 25..30
<223> X = absent or L, I, V

<400> 5
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            20                  25                  30  

<210> 6
<211> 40
<212> PRT
<213> Artificial Sequence


<220> 
<223> Metal cation ligand amino acid motif k=4

<220> 
<221> VARIANT
<222> 1
<223> X = G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R, U, O

<220> 
<221> VARIANT
<222> 2..4
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      U, O

<220> 
<221> VARIANT
<222> 5..10
<223> X = absent or L, I, V

<220> 
<221> VARIANT
<222> 11
<223> X = G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R, U, O

<220> 
<221> VARIANT
<222> 12..14
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      U, O

<220> 
<221> VARIANT
<222> 15..20
<223> X = absent or L, I, V

<220> 
<221> VARIANT
<222> 21
<223> X = G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R, U, O

<220> 
<221> VARIANT
<222> 22..24
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      U, O

<220> 
<221> VARIANT
<222> 25..30
<223> X = absent or L, I, V

<220> 
<221> VARIANT
<222> 31
<223> X = G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R, U, O

<220> 
<221> VARIANT
<222> 32..34
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      U, O

<220> 
<221> VARIANT
<222> 35..40
<223> X = absent or L, I, V

<400> 6
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            20                  25                  30          
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40  

<210> 7
<211> 50
<212> PRT
<213> Artificial Sequence


<220> 
<223> Metal cation ligand amino acid motif k=5

<220> 
<221> VARIANT
<222> 1
<223> X = G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R, U, O

<220> 
<221> VARIANT
<222> 2..4
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      U, O

<220> 
<221> VARIANT
<222> 5..10
<223> X = absent or L, I, V

<220> 
<221> VARIANT
<222> 11
<223> X = G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R, U, O

<220> 
<221> VARIANT
<222> 12..14
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      U, O

<220> 
<221> VARIANT
<222> 15..20
<223> X = absent or L, I, V

<220> 
<221> VARIANT
<222> 21
<223> X = G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R, U, O

<220> 
<221> VARIANT
<222> 22..24
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      U, O

<220> 
<221> VARIANT
<222> 25..30
<223> X = absent or L, I, V

<220> 
<221> VARIANT
<222> 31
<223> X = G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R, U, O

<220> 
<221> VARIANT
<222> 32..34
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      U, O

<220> 
<221> VARIANT
<222> 35..40
<223> X = absent or L, I, V

<220> 
<221> VARIANT
<222> 41
<223> X = G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R, U, O

<220> 
<221> VARIANT
<222> 42..44
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      U, O

<220> 
<221> VARIANT
<222> 45..50
<223> X is absent or L, I, V

<400> 7
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            20                  25                  30          
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40                  45              
Xaa Xaa 
    50  

<210> 8
<211> 60
<212> PRT
<213> Artificial Sequence


<220> 
<223> Metal cation ligand amino acid motif k=6

<220> 
<221> VARIANT
<222> 1
<223> X = G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R, U, O

<220> 
<221> VARIANT
<222> 2..4
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      U, O

<220> 
<221> VARIANT
<222> 5..10
<223> X = absent or L, I, V

<220> 
<221> VARIANT
<222> 11
<223> X = G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R, U, O

<220> 
<221> VARIANT
<222> 12..14
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      U, O

<220> 
<221> VARIANT
<222> 15..20
<223> X = absent or L, I, V

<220> 
<221> VARIANT
<222> 21
<223> X = G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R, U, O

<220> 
<221> VARIANT
<222> 22..24
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      U, O

<220> 
<221> VARIANT
<222> 25..30
<223> X = absent or L, I, V

<220> 
<221> VARIANT
<222> 31
<223> X = G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R, U, O

<220> 
<221> VARIANT
<222> 32..34
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      U, O

<220> 
<221> VARIANT
<222> 35..40
<223> X = absent or L, I, V

<220> 
<221> VARIANT
<222> 41
<223> X = G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R, U, O

<220> 
<221> VARIANT
<222> 42..44
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      U, O

<220> 
<221> VARIANT
<222> 45..50
<223> X = absent or L, I, V

<220> 
<221> VARIANT
<222> 51
<223> X = G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R, U, O

<220> 
<221> VARIANT
<222> 52..54
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      U, O

<220> 
<221> VARIANT
<222> 55..60
<223> X = absent or L, I, V

<400> 8
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            20                  25                  30          
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40                  45              
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
    50                  55                  60  

<210> 9
<211> 20
<212> PRT
<213> Artificial Sequence


<220> 
<223> Spaced histidine tag k=2

<220> 
<221> VARIANT
<222> 2..4
<223> X = absent or H

<220> 
<221> VARIANT
<222> 5..10
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      L, I, V, U, O

<220> 
<221> VARIANT
<222> 12..14
<223> X = absent or H

<220> 
<221> VARIANT
<222> 15..20
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      L, I, V, U, O

<400> 9
His Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa His Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      
Xaa Xaa Xaa Xaa 
            20  

<210> 10
<211> 30
<212> PRT
<213> Artificial Sequence


<220> 
<223> Spaced histidine tag k=3

<220> 
<221> VARIANT
<222> 2..4
<223> X = absent or H

<220> 
<221> VARIANT
<222> 5..10
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      L, I, V, U, O

<220> 
<221> VARIANT
<222> 12..14
<223> X = absent or H

<220> 
<221> VARIANT
<222> 15..20
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      L, I, V, U, O

<220> 
<221> VARIANT
<222> 22..24
<223> X = absent or H

<220> 
<221> VARIANT
<222> 25..30
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      L, I, V, U, O

<400> 10
His Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa His Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      
Xaa Xaa Xaa Xaa His Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            20                  25                  30  

<210> 11
<211> 40
<212> PRT
<213> Artificial Sequence


<220> 
<223> Spaced histidine tag k=4

<220> 
<221> VARIANT
<222> 2..4
<223> X = absent or H

<220> 
<221> VARIANT
<222> 5..10
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      L, I, V, U, O

<220> 
<221> VARIANT
<222> 12..14
<223> X = absent or H

<220> 
<221> VARIANT
<222> 15..20
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      L, I, V, U, O

<220> 
<221> VARIANT
<222> 22..24
<223> X = absent or H

<220> 
<221> VARIANT
<222> 25..30
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      L, I, V, U, O

<220> 
<221> VARIANT
<222> 32..34
<223> X = absent or H

<220> 
<221> VARIANT
<222> 35..40
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      L, I, V, U, O

<400> 11
His Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa His Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      
Xaa Xaa Xaa Xaa His Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa His Xaa 
            20                  25                  30          
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40  

<210> 12
<211> 50
<212> PRT
<213> Artificial Sequence


<220> 
<223> Spaced histidine tag k=5

<220> 
<221> VARIANT
<222> 2..4
<223> X = absent or H

<220> 
<221> VARIANT
<222> 5..10
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      L, I, V, U, O

<220> 
<221> VARIANT
<222> 12..14
<223> X = absent or H

<220> 
<221> VARIANT
<222> 15..20
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      L, I, V, U, O

<220> 
<221> VARIANT
<222> 22..24
<223> X = absent or H

<220> 
<221> VARIANT
<222> 25..30
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      L, I, V, U, O

<220> 
<221> VARIANT
<222> 32..34
<223> X = absent or H

<220> 
<221> VARIANT
<222> 35..40
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      L, I, V, U, O

<220> 
<221> VARIANT
<222> 42..44
<223> X = absent or H

<220> 
<221> VARIANT
<222> 45..50
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      L, I, V, U, O

<400> 12
His Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa His Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      
Xaa Xaa Xaa Xaa His Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa His Xaa 
            20                  25                  30          
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa His Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40                  45              
Xaa Xaa 
    50  

<210> 13
<211> 60
<212> PRT
<213> Artificial Sequence


<220> 
<223> Spaced histidine tag k=6

<220> 
<221> VARIANT
<222> 2..4
<223> X = absent or H

<220> 
<221> VARIANT
<222> 5..10
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      L, I, V, U, O

<220> 
<221> VARIANT
<222> 12..14
<223> X = absent or H

<220> 
<221> VARIANT
<222> 15..20
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      L, I, V, U, O

<220> 
<221> VARIANT
<222> 22..24
<223> X = absent or H

<220> 
<221> VARIANT
<222> 25..30
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      L, I, V, U, O

<220> 
<221> VARIANT
<222> 32..34
<223> X = absent or H

<220> 
<221> VARIANT
<222> 35..40
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      L, I, V, U, O

<220> 
<221> VARIANT
<222> 42..44
<223> X = absent or H

<220> 
<221> VARIANT
<222> 45..50
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      L, I, V, U, O

<220> 
<221> VARIANT
<222> 52..54
<223> X = absent or H

<220> 
<221> VARIANT
<222> 55..60
<223> X = absent or G, A, F, W, M, Y, C, D, E, Q, H, K, P, S, T, N, R,
      L, I, V, U, O

<400> 13
His Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa His Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      
Xaa Xaa Xaa Xaa His Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa His Xaa 
            20                  25                  30          
Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa His Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40                  45              
Xaa Xaa His Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
    50                  55                  60  

<210> 14
<211> 266
<212> PRT
<213> Artificial Sequence


<220> 
<223> His-GFP

<400> 14
Met Gly Ser Ser His His His His His His Gly Ser Ser Val Ser Lys 
1               5                   10                  15      
Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp 
            20                  25                  30          
Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly Glu Gly Glu Gly 
        35                  40                  45              
Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly 
    50                  55                  60                  
Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly 
65                  70                  75                  80  
Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe 
                85                  90                  95      
Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Ser 
            100                 105                 110         
Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu 
        115                 120                 125             
Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys 
    130                 135                 140                 
Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Phe Asn Ser 
145                 150                 155                 160 
His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala 
                165                 170                 175     
Asn Phe Lys Ile Arg His Asn Val Glu Asp Gly Ser Val Gln Leu Ala 
            180                 185                 190         
Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu 
        195                 200                 205             
Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu Ser Lys Asp Pro 
    210                 215                 220                 
Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala 
225                 230                 235                 240 
Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Gly Ile Glu Glu Asn 
                245                 250                 255     
Leu Tyr Phe Gln Ser Asn Ile Gly Ser Gly 
            260                 265     

<210> 15
<211> 591
<212> PRT
<213> Artificial Sequence


<220> 
<223> His-PercevalHR

<400> 15
Met Lys His His His His His His His Gly Ala Ser Met Lys Lys Val 
1               5                   10                  15      
Glu Ser Ile Ile Arg Pro Glu Lys Leu Glu Ile Val Lys Lys Ala Leu 
            20                  25                  30          
Ser Asp Ala Gly Tyr Val Gly Met Thr Val Ser Glu Val Lys Gly Ser 
        35                  40                  45              
Gly Val Gln Gly Gly Ile Phe Glu Arg Tyr Arg Gly Arg Val Tyr Ser 
    50                  55                  60                  
Ala Gly Tyr Asn Ser Asp Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys 
65                  70                  75                  80  
Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly 
                85                  90                  95      
Gly Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp 
            100                 105                 110         
Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Phe Gln Ser Lys 
        115                 120                 125             
Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu 
    130                 135                 140                 
Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
145                 150                 155                 160 
Gly Gly Ser Gly Gly Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly 
                165                 170                 175     
Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys 
            180                 185                 190         
Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu 
        195                 200                 205             
Thr Leu Lys Leu Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro 
    210                 215                 220                 
Thr Leu Val Thr Thr Leu Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr 
225                 230                 235                 240 
Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu 
                245                 250                 255     
Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr 
            260                 265                 270         
Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg 
        275                 280                 285             
Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly 
    290                 295                 300                 
His Lys Leu Glu Tyr Asn Gly Thr Ile Val Asp Leu Ile Pro Lys Val 
305                 310                 315                 320 
Lys Ile Glu Leu Val Val Lys Glu Glu Asp Val Asp Asn Val Ile Asp 
                325                 330                 335     
Ile Ile Cys Glu Asn Ala Arg Thr Gly Asn Pro Gly Asp Gly Lys Ile 
            340                 345                 350         
Phe Val Ile Pro Val Glu Arg Val Val Arg Val Arg Thr Lys Glu Glu 
        355                 360                 365             
Gly Ala Ser Gly Gly Gly Ser Gly Gly Gly Gly Ala Ser Gly Met Lys 
    370                 375                 380                 
Lys Val Glu Ala Ile Ile Arg Pro Glu Lys Leu Glu Ile Val Lys Lys 
385                 390                 395                 400 
Ala Leu Ser Asp Ala Gly Tyr Val Gly Met Thr Val Ser Glu Val Lys 
                405                 410                 415     
Gly Arg Gly Ala Gly Gly Gly Asp Leu Ile Pro Lys Val Lys Ile Glu 
            420                 425                 430         
Leu Val Val Lys Glu Glu Asp Val Asp Asn Val Ile Asp Ile Ile Cys 
        435                 440                 445             
Glu Asn Ala Arg Thr Gly Asn Pro Gly Asp Gly Lys Ile Phe Val Ile 
    450                 455                 460                 
Pro Val Glu Arg Ile Val Arg Val Arg Thr Lys Glu Glu Gly Ala Ser 
465                 470                 475                 480 
Gly Gly Gly Gly Gly Ser Gly Gly Ala Ser Gly Met Lys Lys Val Glu 
                485                 490                 495     
Ala Ile Ile Arg Pro Glu Lys Leu Glu Ile Val Lys Lys Ala Leu Asn 
            500                 505                 510         
Asp Asp Gly Tyr Val Gly Met Thr Val Ser Glu Val Lys Gly Arg Gly 
        515                 520                 525             
Ala Gly Gly Gly Asp Leu Ile Pro Lys Val Lys Ile Glu Leu Val Val 
    530                 535                 540                 
Lys Glu Glu Asp Val Asp Asn Ile Ile Asp Ile Ile Cys Glu Asn Ala 
545                 550                 555                 560 
Arg Thr Gly Asn Pro Gly Asp Gly Lys Ile Phe Val Ile Pro Val Glu 
                565                 570                 575     
Arg Val Val Arg Pro Arg Thr Lys Glu Glu Gly Lys Glu Ala Leu 
            580                 585                 590     

<210> 16
<211> 156
<212> PRT
<213> Artificial Sequence


<220> 
<223> enzyme Sortase A

<400> 16
Met Gln Ala Lys Pro Gln Ile Pro Lys Asp Lys Ser Lys Val Ala Gly 
1               5                   10                  15      
Tyr Ile Glu Ile Pro Asp Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly 
            20                  25                  30          
Pro Ala Thr Arg Glu Gln Leu Asn Arg Gly Val Ser Phe Ala Glu Glu 
        35                  40                  45              
Asn Glu Ser Leu Asp Asp Gln Asn Ile Ser Ile Ala Gly His Thr Phe 
    50                  55                  60                  
Ile Asp Arg Pro Asn Tyr Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys 
65                  70                  75                  80  
Gly Ser Met Val Tyr Phe Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys 
                85                  90                  95      
Met Thr Ser Ile Arg Asn Val Lys Pro Thr Ala Val Glu Val Leu Asp 
            100                 105                 110         
Glu Gln Lys Gly Lys Asp Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp 
        115                 120                 125             
Tyr Asn Glu Glu Thr Gly Val Trp Glu Thr Arg Lys Ile Phe Val Ala 
    130                 135                 140                 
Thr Glu Val Lys Leu Glu His His His His His His 
145                 150                 155     

