MULTILAYER ENGINEERED HEART MUSCLE

(19)

(11)

EP 3 988 138 A8

(12)	CORRECTED EUROPEAN PATENT APPLICATION
	Note: Bibliography reflects the latest situation

(15)	Correction information:
	Corrected version no 1 (W1 A1)

(48)	Corrigendum issued on:
	07.09.2022 Bulletin 2022/36

(43)	Date of publication:
	27.04.2022 Bulletin 2022/17

(21)	Application number: 20203316.3

(22)	Date of filing: 22.10.2020

(51)

International Patent Classification (IPC):

A61L 27/24^(2006.01)
A61L 27/52^(2006.01)

A61L 27/38^(2006.01)

(52)	Cooperative Patent Classification (CPC):
	A61L 27/24; A61L 27/3826; A61L 27/3886; A61L 27/3895; A61L 27/52; A61L 2430/20

(84)	Designated Contracting States:
	AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
	Designated Extension States:
	BA ME
	Designated Validation States:
	KH MA MD TN

(71)	Applicant: Georg-August-Universität Göttingen Stiftung Öffentlichen Rechts, Universitätsmedizin
	37075 Göttingen (DE)

(72)	Inventors:
	ZIMMERMANN, Wolfram-Hubertus 37073 Göttingen (DE) TIBURCY, Malte 37083 Göttingen (DE) MEYER, Tim 37083 Göttingen (DE)

(74)	Representative: Wichmann, Hendrik
	Wuesthoff & Wuesthoff Patentanwälte PartG mbB Schweigerstraße 2 81541 München 81541 München (DE)

(54)	MULTILAYER ENGINEERED HEART MUSCLE

(57) The application describes a method for manufacturing a multilayer engineered heart muscle. Specifically, the method relates to (i) providing a liquid reconstitution mixture in a mould and (ii) culturing said mixture. The method is characterized by a sequential addition of one or more further liquid reconstitution mixtures in order to obtain a multilayer engineered heart muscle. Said muscle ideally has the form of a patch, a pouch, or a cylinder. Furthermore, the application relates to a multilayer engineered heart muscle comprising collagen, cardiac myocytes and non-myocytes originating from at least 2 layers. Said multilayer engineered heart muscle forms the basis for several in vitro and in vivo applications such as the production of a multilayer engineered heart muscle for use in a patient, for example for use in heart repair.