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EP 4 018 002 A1

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(43) Date of publication: 29.06.2022 Bulletin 2022/26 (21) Application number: 20854178.9 (22) Date of filing: 18.08.2020

(51) International Patent Classification (IPC):  C12Q 1/6874(2018.01) (52) Cooperative Patent Classification (CPC): C12Q 1/6874   C-Sets: C12Q 1/6874, C12Q 2525/113; (86) International application number: PCT/US2020/046851 (87) International publication number: WO 2021/034853 (25.02.2021 Gazette 2021/08)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA ME Designated Validation States: KH MA MD TN (30) Priority: 21.08.2019 US 201962890065 P (71) Applicants: Illumina, Inc. San Diego, CA 92122 (US) Illumina Singapore Pte. Ltd. Singapore 757716 (SG)

(72) Inventors: ZHAO, Yannan San Diego, CA 92122 (US) PUGLIESE, Kaitlin San Diego, CA 92122 (US) GARCIA, Erin San Diego, CA 92122 (US) VINCENT, Ludovic San Diego, CA 92122 (US) PRABHU, Anmiv San Diego, CA 92122 (US) GRAVINA, Silvia San Diego, CA 92122 (US) PEISAJOVICH, Sergio San Diego, CA 92122 (US) TEO, Yin, Nah 18 757716 Singapore (SG) YANG, Xiangyuan 18 757716 Singapore (SG) MANDELL, Jeffrey San Diego, CA 92122 (US) STUTCHMAN, Jonathon San Diego, CA 92122 (US) (74) Representative: Murgitroyd & Company  Murgitroyd House 165-169 Scotland Street Glasgow G5 8PL Glasgow G5 8PL (GB)