(19)

(11)

EP 4 073 248 A1

(12)

(43) Date of publication: 19.10.2022 Bulletin 2022/42 (21) Application number: 20900344.1 (22) Date of filing: 11.12.2020

(51) International Patent Classification (IPC):  C12N 15/10(2006.01) C12Q 1/6834(2018.01) C23C 8/06(2006.01) G01N 1/40(2006.01) C12Q 1/66(2006.01) B01J 20/10(2006.01) A61K 47/32(2006.01) A61L 27/52(2006.01) (52) Cooperative Patent Classification (CPC): C12N 15/1003 (86) International application number: PCT/US2020/064508 (87) International publication number: WO 2021/119425 (17.06.2021 Gazette 2021/24)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA ME Designated Validation States: KH MA MD TN (30) Priority: 13.12.2019 US 201962947969 P (71) Applicant: Pacific Biosciences Of California, Inc. Menlo Park, CA 94025 (US)	(72) Inventors: LIU, Kelvin, Jeng-Fang Baltimore, MD 21202 (US) KILBURN, John Duncan Baltimore, MD 21202 (US) (74) Representative: London IP Ltd  100 High street Milford on Sea Lymington, Hampshire SO41 0QE Lymington, Hampshire SO41 0QE (GB)