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(11)

EP 4 136 229 A1

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(43) Date of publication: 22.02.2023 Bulletin 2023/08 (21) Application number: 21788834.6 (22) Date of filing: 16.04.2021

(51) International Patent Classification (IPC):  C12N 15/11(2006.01) C12Q 1/6876(2018.01) C12Q 1/70(2006.01) C12Q 1/6844(2018.01) C12Q 1/686(2018.01) (52) Cooperative Patent Classification (CPC): C12Q 1/701 (86) International application number: PCT/US2021/027844 (87) International publication number: WO 2021/212088 (21.10.2021 Gazette 2021/42)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA ME Designated Validation States: KH MA MD TN (30) Priority: 16.04.2020 US 202063010840 P (71) Applicant: Diacarta LLC Richmond, CA 94806 (US)	(72) Inventors: SUN, Qing Fairfield, CA 94524 (US) SHA, Michael, Y Castro Valley, CA 94552 (US) REN, Hui San Francisco, CA 94080 (US) LI, Jonathan Danville, CA 94506 (US) ZHANG, Aiguo San Ramon, CA 94583 (US) (74) Representative: Schüssler, Andrea  Kanzlei Huber & Schüssler Truderinger Strasse 246 81825 München 81825 München (DE)