(19)

(11)

EP 4 139 444 A2

(12)

(88) Date of publication A3: 03.03.2022 (43) Date of publication: 01.03.2023 Bulletin 2023/09 (21) Application number: 21792151.9 (22) Date of filing: 23.04.2021

(51) International Patent Classification (IPC):  C12N 9/02(2006.01) C12N 15/00(2006.01) C07H 21/04(2006.01) (52) Cooperative Patent Classification (CPC): C12N 9/00; C12Y 102/99006; C12N 9/0008; C12N 9/1029; C12Y 203/01174; C12N 9/14; C12N 9/88; C12N 9/0006; C12Y 101/05; C12Y 103/01038; C12N 9/001; C12N 9/1096; C12Y 102/0101; C12P 13/001; C12P 7/18 (86) International application number: PCT/US2021/028768 (87) International publication number: WO 2021/216952 (28.10.2021 Gazette 2021/43)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA ME Designated Validation States: KH MA MD TN (30) Priority: 24.04.2020 US 202063015428 P (71) Applicant: Genomatica, Inc. San Diego, CA 92121 (US)

(72) Inventors: SHAH, Amit, Mahendra San Diego, CA 92121 (US) ZHANG, Deqiang San Diego, CA 92121 (US) WARNER, Joseph, Roy San Diego, CA 92121 (US) GRIFFIN, Benjamin, Matthew San Diego, CA 92121 (US) KOMOR, Russell, Scott San Diego, CA 92121 (US) NAGARAJAN, Harish San Diego, CA 92121 (US) GHATAK, Sankha San Diego, CA 92121 (US) RICHARDSON, Toby, Howard San Diego, CA 92121 (US) (74) Representative: Lahrtz, Fritz  Simmons & Simmons LLP Lehel Carré Thierschplatz 6 80538 München 80538 München (DE)