(19)

(11)

EP 4 165 177 A1

(12)

(43) Date of publication: 19.04.2023 Bulletin 2023/16 (21) Application number: 21799712.1 (22) Date of filing: 06.05.2021

(51) International Patent Classification (IPC):  C12N 9/22(2006.01) C12N 15/10(2006.01) C12N 15/113(2010.01) C12N 15/63(2006.01) (52) Cooperative Patent Classification (CPC): C12N 9/22; C12N 15/102; C12N 2310/20; C07K 2319/09; C07K 2319/21; C07K 2319/60 (86) International application number: PCT/US2021/031143 (87) International publication number: WO 2021/226369 (11.11.2021 Gazette 2021/45)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA ME Designated Validation States: KH MA MD TN (30) Priority: 08.05.2020 US 202063022321 P 29.05.2020 US 202063032465 P 19.11.2020 US 202063116156 P 27.04.2021 US 202163180587 P (71) Applicant: Metagenomi, Inc. Emeryville, CA 94608 (US)

(72) Inventors: THOMAS, Brian Emeryville, California 94608 (US) BROWN, Christopher Emeryville, California 94608 (US) KANTOR, Rose Emeryville, California 94608 (US) DEVOTO, Audra Emeryville, California 94608 (US) BUTTERFIELD, Cristina Emeryville, California 94608 (US) ALEXANDER, Lisa Emeryville, California 94608 (US) GOLTSMAN, Daniela S.A. Emeryville, California 94608 (US) LIU, Jason Emeryville, California 94608 (US) LAMOTHE, Rebecca Emeryville, California 94608 (US) ESPINOSA, Diego Emeryville, California 94608 (US) STORLIE, Meghan Emeryville, California 94608 (US) COST, Greg Emeryville, California 94608 (US) (74) Representative: Mewburn Ellis LLP  Aurora Building Counterslip Bristol BS1 6BX Bristol BS1 6BX (GB)