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(11)

EP 4 185 702 A1

(12)

(43) Date of publication: 31.05.2023 Bulletin 2023/22 (21) Application number: 21846426.1 (22) Date of filing: 22.07.2021

(51) International Patent Classification (IPC):  C12N 15/86(2006.01) A61P 21/00(2006.01) A61K 48/00(2006.01) (52) Cooperative Patent Classification (CPC): A61P 21/00; C12N 15/86; C12N 2750/14143; C12N 2750/14122; C07K 14/005; C12N 2800/40; C12N 2830/008; C12N 2830/42; C12N 2830/50; C12N 2310/20; C40B 40/08; C40B 40/02; C12N 15/1058; A61K 48/0008; A61K 48/0041 (86) International application number: PCT/US2021/042812 (87) International publication number: WO 2022/020616 (27.01.2022 Gazette 2022/04)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA ME Designated Validation States: KH MA MD TN (30) Priority: 22.07.2020 US 202063055265 P 29.10.2020 US 202063107394 P 02.05.2021 US 202163183038 P (71) Applicants: The Broad Institute, Inc. Cambridge, MA 02142 (US) President And Fellows Of Harvard College Cambridge, Massachusetts 02138 (US) MASSACHUSETTS INSTITUTE OF TECHNOLOGY Cambridge, MA 02139 (US)

(72) Inventors: SABETI, Pardis Cambridge, Massachusetts 02138 (US) TABEBORDBAR, Mohammadsharif Cambridge, Massachusetts 02142 (US) YE, Simon Cambridge, Massachusetts 02139 (US) (74) Representative: Impact Intellectual Property LLP  1 Sans Walk London EC1R 0LT London EC1R 0LT (GB)