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(11)

EP 4 200 411 A1

(12)

(43) Date of publication: 28.06.2023 Bulletin 2023/26 (21) Application number: 21769161.7 (22) Date of filing: 23.08.2021

(51) International Patent Classification (IPC):  C12N 9/12(2006.01) C12Q 1/6844(2018.01) C12P 19/34(2006.01) C12Q 1/6869(2018.01) (52) Cooperative Patent Classification (CPC): C12P 19/34; C12Y 207/07031; C12N 9/1264 (86) International application number: PCT/GB2021/052189 (87) International publication number: WO 2022/038386 (24.02.2022 Gazette 2022/08)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA ME Designated Validation States: KH MA MD TN (30) Priority: 21.08.2020 GB 202013102 (71) Applicant: Nuclera Nucleics Ltd Cambridge, Cambridgeshire CB24 9NP (GB)

(72) Inventors: OST, Tobias William Barr Cambridge Science Park Milton Road Cambridge CB4 0GD (GB) MCINROY, Gordon Ross Cambridge Science Park Milton Road Cambridge CB4 0GD (GB) GABER, Zachary Beyer Cambridge Science Park Milton Road Cambridge CB4 0GD (GB) SWERDLOW, Harold Cambridge Science Park Milton Road Cambridge CB4 0GD (GB) TOGNOLONI, Cecilia Cambridge Science Park Milton Road Cambridge CB4 0GD (GB) (74) Representative: Barnes, Colin Lloyd et al Nuclera Nucleics Ltd One Vision Park Station Road, Impington Cambridge CB24 9NP Cambridge CB24 9NP (GB)