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(11)

EP 4 263 827 A2

(12)

(88) Date of publication A3: 18.08.2022 (43) Date of publication: 25.10.2023 Bulletin 2023/43 (21) Application number: 21857017.4 (22) Date of filing: 15.12.2021

(51) International Patent Classification (IPC):  C12N 15/10(2006.01) C12Q 1/6806(2018.01) C12N 15/11(2006.01) G16B 25/20(2019.01) (52) Cooperative Patent Classification (CPC): C12N 15/1031; G16B 30/20   C-Sets: C12N 15/1031, C12Q 2521/313, C12Q 2521/501, C12Q 2525/301; (86) International application number: PCT/US2021/010063 (87) International publication number: WO 2022/132198 (23.06.2022 Gazette 2022/25)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA ME Designated Validation States: KH MA MD TN (30) Priority: 15.12.2020 US 202063125530 P 23.06.2021 US 202163213807 P 23.06.2021 US 202163213859 P (71) Applicant: New England Biolabs, Inc. Ipswich, MA 01938-2723 (US)	(72) Inventors: LOHMAN, Gregory Ipswich, MA 01938 (US) POTAPOV, Vladimir Ipswich, MA 01938 (US) PRYOR, John, M. Ipswich, MA 01938 (US) KUCERA, Rebecca Ipswich, MA 01938 (US) BILOTTI, Katharina Ipswich, MA 01938 (US) MORGAN, Richard, D. Ipswich, MA 01938 (US) (74) Representative: Griffin, Philippa Jane et al Mathys & Squire The Shard 32 London Bridge Street London SE1 9SG London SE1 9SG (GB)