(19)
(11) EP 4 274 894 A2

(12)

(88) Date of publication A3:
11.08.2022

(43) Date of publication:
15.11.2023 Bulletin 2023/46

(21) Application number: 22702103.7

(22) Date of filing: 11.01.2022
(51) International Patent Classification (IPC): 
C12N 15/11(2006.01)
C12N 9/12(2006.01)
 C12N 15/10(2006.01)
C12N 9/22(2006.01)
(52) Cooperative Patent Classification (CPC):
C12N 15/111; C12N 9/22; C12N 9/1276; C12N 2310/20; C12N 2310/3519; C12N 15/102; C12Y 207/07049; C12Y 301/00
(86) International application number:
PCT/US2022/012054
(87) International publication number:
WO 2022/150790 (14.07.2022 Gazette 2022/28)
(84) Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated Extension States:
BA ME
Designated Validation States:
KH MA MD TN

(30) Priority: 11.01.2021 US 202163136194 P
16.04.2021 US 202163176180 P
16.04.2021 US 202163176202 P
28.05.2021 US 202163194865 P
28.05.2021 US 202163194913 P
09.08.2021 US 202163231230 P
14.10.2021 US 202163255897 P

(71) Applicants:
  • The Broad Institute, Inc.
    Cambridge, MA 02142 (US)
  • President and Fellows of Harvard College
    Cambridge, MA 02138 (US)
  • The Trustees of Princeton University
    Princeton, NJ 08540 (US)
  • The Regents of The University of California
    Oakland CA 94607-5200 (US)

(72) Inventors:
  • LIU, David, R.
    Cambridge, MA 02142 (US)
  • CHEN, Peter, J.
    Cambridge, MA 02142 (US)
  • ADAMSON, Brittany
    Princeton, NJ 08540 (US)
  • HUSSMANN, Jeffrey
    Oakland, CA 94607-5200 (US)

(74) Representative: Atkins, James Gordon John et al
Kilburn & Strode LLP Lacon London 84 Theobalds Road
London WC1X 8NL
London WC1X 8NL (GB)

   


(54) PRIME EDITOR VARIANTS, CONSTRUCTS, AND METHODS FOR ENHANCING PRIME EDITING EFFICIENCY AND PRECISION