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EP 4 281 586 A2

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(88) Date of publication A3: 01.09.2022 (43) Date of publication: 29.11.2023 Bulletin 2023/48 (21) Application number: 22743385.1 (22) Date of filing: 25.01.2022

(51) International Patent Classification (IPC):  C12Q 1/6874(2018.01) C12Q 1/6881(2018.01) C12Q 1/6876(2018.01) (52) Cooperative Patent Classification (CPC): C12N 15/1075; C12N 15/1013; G01N 33/54326; G01N 33/569; G01N 33/545   C-Sets: C12N 15/1075, C12Q 2563/179; (86) International application number: PCT/US2022/013720 (87) International publication number: WO 2022/159896 (28.07.2022 Gazette 2022/30)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA ME Designated Validation States: KH MA MD TN (30) Priority: 25.01.2021 US 202163141064 P (71) Applicant: Fluent Biosciences Inc. Watertown, MA 02472 (US)	(72) Inventors: XUE, Yi Shrewsbury, MA 01545 (US) FONTANEZ, Kristina Arlington, MA 02476 (US) KIANI, Sepher Watertown, MA 02472 (US) MELTZER, Robert Belmont, MA 02478 (US) (74) Representative: Graham Watt & Co LLP  St. Botolph's House 7-9 St. Botolph's Road Sevenoaks TN13 3AJ Sevenoaks TN13 3AJ (GB)