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EP 4 334 468 A2

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(88) Date of publication A3: 12.01.2023 (43) Date of publication: 13.03.2024 Bulletin 2024/11 (21) Application number: 22799461.3 (22) Date of filing: 03.05.2022

(51) International Patent Classification (IPC):  C12Q 1/6806(2018.01) C12Q 1/00(2006.01) C12Q 1/6874(2018.01) C12N 9/22(2006.01) C12Q 1/68(2018.01) C12Q 1/6886(2018.01) (52) Cooperative Patent Classification (CPC): C12Q 1/6886; G16B 20/00; G16B 40/00 (86) International application number: PCT/US2022/027536 (87) International publication number: WO 2022/235718 (10.11.2022 Gazette 2022/45)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA ME Designated Validation States: KH MA MD TN (30) Priority: 03.05.2021 US 202163183610 P 06.08.2021 US 202163230584 P (71) Applicants: The Regents of The University of California Oakland CA 94607-5200 (US) Wellcome Sanger Institute Hinxton, Cambridgeshire CB10 1SA (GB)

(72) Inventors: AHITUV, Nadav Walnut Creek, CA (US) YIZHAR-BARNEA, Ofer Foster City, CA 94404 (US) GEORGAKOPOULOS-SOARES, Ilias San Francisco, CA 94158 (US) MOURATIDIS, Ioannis 1150 Brussels (BE) HEMBERG, Martin Weston, MA 02493 (US) (74) Representative: Leonard, Thomas Charles et al Kilburn & Strode LLP Lacon London 84 Theobalds Road London WC1X 8NL London WC1X 8NL (GB)