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(11) | EP 4 343 304 A3 |
(12) | EUROPEAN PATENT APPLICATION |
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(54) | FLOW CYTOMETER, LASER OPTICS ASSEMBLY THEREOF, AND METHODS OF ASSEMBLING THE SAME |
(57) A flow cytometer is disclosed. It comprises a flow cell defining a flow direction;
a collimation assembly including laser diode and a collimating lens configured to
produce a laser beam along an axis; a positive cylindrical lens disposed on the axis
configured to receive the laser beam from the collimation assembly; and a negative
cylindrical lens disposed on the axis and configured to receive the laser beam from
the positive cylindrical lens. A cylindrical objective lens is disposed on the axis
and is configured to receive the laser beam from the negative cylindrical lens and
project the laser beam onto the flow cell such that the laser beam incident on the
flow cell defines a first beam waist 1/e2 diameter in a direction parallel to the flow direction of the flow cell of 6.7 µm
to 9 µm and a second beam 1/e2 diameter in a direction perpendicular to the flow direction of the flow cell of 190
µm to 210 µm.
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