(19)
(11) EP 4 392 563 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
02.04.2025 Bulletin 2025/14

(45) Mention of the grant of the patent:
26.02.2025 Bulletin 2025/09

(21) Application number: 23765455.3

(22) Date of filing: 31.08.2023
(51) International Patent Classification (IPC): 
C12N 15/113(2010.01)
C12N 9/22(2006.01)
C12N 15/62(2006.01)
 C07K 14/26(2006.01)
C12N 9/78(2006.01)
(52) Cooperative Patent Classification (CPC):
C07K 14/26; C12N 15/113; C12N 2310/20; C12N 9/78; C12N 9/22; C12N 15/62
(86) International application number:
PCT/EP2023/073881
(87) International publication number:
WO 2024/047151 (07.03.2024 Gazette 2024/10)

(54)

A NOVEL TYPE OF CRISPR/CAS SYSTEM

NEUARTIGES CRISPR/CAS-SYSTEM

NOUVEAU TYPE DE SYSTÈME CRISPR/CAS

(84) Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC ME MK MT NL NO PL PT RO RS SE SI SK SM TR

(30) Priority: 31.08.2022 GB 202212688

(43) Date of publication of application:
03.07.2024 Bulletin 2024/27

(60) Divisional application:
25159758.9

(73) Proprietor: SNIPR Biome ApS
2100 Copenhagen (DK)

(72) Inventors:
  • SEMSEY, Szabolcs
    2100 Copenhagen (DK)
  • MOUGIAKOS, Ioannis
    2100 Copenhagen (DK)
  • SØNDBERG, Emilie
    2100 Copenhagen (DK)
  • CLUBE, Jasper
    2100 Copenhagen (DK)

(74) Representative: CMS Cameron McKenna Nabarro Olswang LLP 
Cannon Place 78 Cannon Street
London EC4N 6AF
London EC4N 6AF (GB)


(56) References cited: : 
   
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  • DATABASE European Nucleotide Archi [online] 23 June 2016 (2016-06-23), ANONYMOUS: "Klebsiella pneumoniae subsp. pneumoniae strain 234-12 plasmid pKpn23412-362, complete sequence", XP093107256, retrieved from EMBL Database accession no. CP011314
  • DATABASE GenBank [online] NCBI; 26 July 2022 (2022-07-26), ANONYMOUS: "Zea mays cultivar Jing724 bHLH transcription factor ZmR1 (ZmR1) gene, - Nucleotide - NCBI", XP093106650, retrieved from NCBI Database accession no. ON256214.1
  • BECKER LAURA ET AL: "Complete Genome Sequence of a CTX-M-15-Producing Klebsiella pneumoniae Outbreak Strain from Multilocus Sequence Type 514", GENOME ANNOUNCEMENTS, vol. 3, no. 4, 9 July 2015 (2015-07-09), US, XP093107138, ISSN: 2169-8287, DOI: 10.1128/genomeA.00742-15
  • DATABASE European Nucleotide Archi [online] 23 June 2016 (2016-06-23), ANONYMOUS: "Klebsiella pneumoniae subsp. pneumoniae strain 234-12 plasmid pKpn23412-362, complete sequence", XP093107256, retrieved from EMBL Database accession no. CP011314
  • SCHLÜTER ANDREAS ET AL: "IncH-Type Plasmid Harboring bla CTX-M-15 , bla DHA-1 , and qnrB4 Genes Recovered from Animal Isolates", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, vol. 58, no. 7, 21 April 2014 (2014-04-21), US, pages 3768 - 3773, XP093106674, ISSN: 0066-4804, DOI: 10.1128/AAC.02695-14
  • DATABASE GenBank [online] NCBI; 26 July 2016 (2016-07-26), ANONYMOUS: "Klebsiella pneumoniae Kp15 plasmid pENVA complete sequence - Nucleotide - NCBI", XP093106685, retrieved from NCBI Database accession no. HG918041.1
  • YOUNG JOSHUA K ET AL: "The repurposing of type I-E CRISPR-Cascade for gene activation in plants", COMMUNICATIONS BIOLOGY, vol. 2, 383, 18 October 2019 (2019-10-18), England, pages 1 - 7, XP055808274, Retrieved from the Internet <URL:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6802105/pdf/42003_2019_Article_637.pdf> [retrieved on 20210527], DOI: 10.1038/s42003-019-0637-6
  • YOUNG JOSHUA K ET AL: "The repurposing of type I-E CRISPR-Cascade for gene activation in plants - supplementary tables", COMMUNICATIONS BIOLOGY, 18 October 2019 (2019-10-18), XP093106642
  • DATABASE GenBank [online] NCBI; 26 July 2022 (2022-07-26), ANONYMOUS: "Zea mays cultivar Jing724 bHLH transcription factor ZmR1 (ZmR1) gene, - Nucleotide - NCBI", XP093106650, retrieved from NCBI Database accession no. ON256214.1
  • MICHELLE L. LUO ET AL: "Repurposing endogenous type I CRISPR-Cas systems for programmable gene repression", NUCLEIC ACIDS RESEARCH, vol. 43, no. 1, 17 October 2014 (2014-10-17), GB, pages 674 - 681, XP055244392, ISSN: 0305-1048, DOI: 10.1093/nar/gku971
  • MICHELLE L LUO ET AL: "SUPPLEMENTARY INFORMATION - Repurposing endogenous Type I CRISPR-Cas systems for programmable gene repression", 17 October 2014 (2014-10-17), XP055408210, Retrieved from the Internet <URL:https://academic.oup.com/nar/article/43/1/674/2903426#supplementary-data> [retrieved on 20170920]
  • LILIANA MEDINA-APARICIO ET AL: "The CRISPR-Cas system in Enterobacteriaceae", PATHOGENS AND DISEASE, vol. 76, no. 1, 9 January 2018 (2018-01-09), XP055591440, DOI: 10.1093/femspd/fty002
  • ZHENG YANLI ET AL: "Endogenous Type I CRISPR-Cas: From Foreign DNA Defense to Prokaryotic Engineering", FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, vol. 8, 4 March 2020 (2020-03-04), XP055880718, DOI: 10.3389/fbioe.2020.00062
   
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