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(11)

EP 4 437 135 A1

(12)

(43) Date of publication: 02.10.2024 Bulletin 2024/40 (21) Application number: 22899249.1 (22) Date of filing: 21.07.2022

(51) International Patent Classification (IPC):  C12Q 1/6874(2018.01) C12Q 1/6837(2018.01) C12N 15/10(2006.01) G01N 33/53(2006.01) (52) Cooperative Patent Classification (CPC): C12Q 1/6816   C-Sets: C12Q 1/6816, C12Q 2521/301, C12Q 2525/307; (86) International application number: PCT/US2022/037791 (87) International publication number: WO 2023/096675 (01.06.2023 Gazette 2023/22)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA ME Designated Validation States: KH MA MD TN (30) Priority: 23.11.2021 WO PCT/US2021/060647 08.03.2022 US 202263317838 P 26.05.2022 US 202263346186 P (71) Applicants: Pleno, Inc. San Diego, CA 92121 (US) Exact Sciences Corporation Madison, Wisconsin 53719 (US)

(72) Inventors: BRODIN, Jeffrey San Diego, California 92121 (US) BERTI, Lorenzo San Diego, California 92121 (US) EIDSON, Donald Brian San Diego, California 92121 (US) SCHLEGEL, Christian San Diego, California 92121 (US) BLUM, Angela San Diego, California 92121 (US) SCHOWALTER, Rachel San Diego, California 92121 (US) VINCENT, Ludovic San Diego, California 92121 (US) VAN ROOYEN, Pieter San Diego, California 92121 (US) STONE, Gavin San Diego, California 92121 (US) ALLAWI, Hatim T. Madison, Wisconsin 53719 (US) (74) Representative: Murgitroyd & Company  165-169 Scotland Street Glasgow G5 8PL Glasgow G5 8PL (GB)