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EP 4 460 569 A1

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(43) Date of publication: 13.11.2024 Bulletin 2024/46 (21) Application number: 23737771.8 (22) Date of filing: 06.01.2023

(51) International Patent Classification (IPC):  C12N 15/52(2006.01) A61Q 13/00(2006.01) A61Q 17/04(2006.01) C12N 9/04(2006.01) C12N 9/12(2006.01) C12N 9/88(2006.01) C12N 15/74(2006.01) C12P 5/00(2006.01) C12P 17/06(2006.01) C12R 1/45(2006.01) A61K 8/99(2017.01) A61Q 15/00(2006.01) C12N 1/20(2006.01) C12N 9/10(2006.01) C12N 9/16(2006.01) C12N 9/90(2006.01) C12N 15/77(2006.01) C12P 7/04(2006.01) C12R 1/15(2006.01) (52) Cooperative Patent Classification (CPC): A61K 8/99; A61Q 13/00; A61Q 15/00; A61Q 17/04; C12N 1/20; C12N 9/88; C12N 9/90; C12N 15/77; C12P 17/06; C12R 2001/15; C12P 5/007; C12Y 402/03108; C12Y 402/03073; C12Y 301/07011; C12N 15/52; C12N 9/1029; C12N 9/1025; C12N 9/0006; C12N 9/1205; C12N 9/1229; C12N 9/1085; C12R 2001/45 (86) International application number: PCT/US2023/060212 (87) International publication number: WO 2023/133490 (13.07.2023 Gazette 2023/28)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC ME MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA Designated Validation States: KH MA MD TN (30) Priority: 07.01.2022 US 202263297302 P 09.06.2022 US 202263350634 P (71) Applicant: Ginkgo Bioworks, Inc. Boston, MA 02210 (US)	(72) Inventors: BALDERA AGUAYO, Pedro Alexis Boston, MA 02210 (US) CHATZIVASILEIOU, Alkiviadis Orfefs Boston, MA 02210 (US) FORMIGHIERI, Cinzia Boston, MA 02210 (US) (74) Representative: Mewburn Ellis LLP  Aurora Building Counterslip Bristol BS1 6BX Bristol BS1 6BX (GB)