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(11)

EP 4 479 406 A1

(12)

(43) Date of publication: 25.12.2024 Bulletin 2024/52 (21) Application number: 23720013.4 (22) Date of filing: 15.02.2023

(51) International Patent Classification (IPC):  C07D 498/04(2006.01) C07H 19/00(2006.01) C09B 19/00(2006.01) C12Q 1/6869(2018.01) C07D 498/14(2006.01) C07H 21/00(2006.01) C12Q 1/6844(2018.01) (52) Cooperative Patent Classification (CPC): C07D 498/14; C12Q 1/6844; C12Q 1/6869; C07D 498/04; C09B 57/00; C07H 19/10; C07H 19/20   C-Sets: C12Q 1/6844, C12Q 2525/113; C12Q 1/6869, C12Q 2525/113; (86) International application number: PCT/US2023/062625 (87) International publication number: WO 2023/159042 (24.08.2023 Gazette 2023/34)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC ME MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA Designated Validation States: KH MA MD TN (30) Priority: 16.02.2022 US 202263310796 P (71) Applicant: Promega Corporation Madison, WI 53711-5399 (US)	(72) Inventors: SHI, Ce Madison, Wisconsin 53711-5399 (US) CAO, Jian Madison, Wisconsin 53711-5399 (US) ZHOU, Wenhui Madison, Wisconsin 53711-5399 (US) (74) Representative: Potter Clarkson  Chapel Quarter Mount Street Nottingham NG1 6HQ Nottingham NG1 6HQ (GB)