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(11)

EP 4 504 970 A1

(12)

(43) Date of publication: 12.02.2025 Bulletin 2025/07 (21) Application number: 23721254.3 (22) Date of filing: 10.04.2023

(51) International Patent Classification (IPC):  C12Q 1/6869(2018.01) (52) Cooperative Patent Classification (CPC): C12Q 1/6869   C-Sets: C12Q 1/6869, C12Q 2521/307, C12Q 2521/525; (86) International application number: PCT/US2023/065602 (87) International publication number: WO 2023/197015 (12.10.2023 Gazette 2023/41)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC ME MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA Designated Validation States: KH MA MD TN (30) Priority: 08.04.2022 US 202263329262 P 10.03.2023 US 202318182122 (71) Applicant: New England Biolabs, Inc. Ipswich, MA 01938-2723 (US)	(72) Inventors: CORREA, Ivan, R., Jr. Ipswich, MA 01938 (US) WOLF, Eric Ipswich, MA 01938 (US) DAI, Nan Ipswich, MA 01938 (US) YIGIT, Erbay Ipswich, MA 01938 (US) GRÜNBERG, Sebastian Ipswich, MA 01938 (US) (74) Representative: Mathys & Squire  The Shard 32 London Bridge Street London SE1 9SG London SE1 9SG (GB)