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EP 4 544 043 A1

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(43) Date of publication: 30.04.2025 Bulletin 2025/18 (21) Application number: 23745674.4 (22) Date of filing: 22.06.2023

(51) International Patent Classification (IPC):  C12N 15/11(2006.01) (52) Cooperative Patent Classification (CPC): C12N 15/11; C12N 2310/351; C12N 2310/321; C12N 2310/322; C12N 2310/3341; C12N 2310/315   C-Sets: C12N 2310/321, C12N 2310/3521; C12N 2310/322, C12N 2310/3533; C12N 2310/322, C12N 2310/3525; (86) International application number: PCT/US2023/026027 (87) International publication number: WO 2023/250112 (28.12.2023 Gazette 2023/52)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC ME MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA Designated Validation States: KH MA MD TN (30) Priority: 22.06.2022 US 202263354602 P 22.06.2022 US 202263354604 P (71) Applicant: Flagship Pioneering Innovations VI, LLC Cambridge, MA 02142 (US)

(72) Inventors: ANASTASSIADIS, Theonie Cambridge, Massachusetts 02142 (US) BUTLER, David, Charles, Donnell Cambridge, Massachusetts 02142 (US) KUBICA, Neil Cambridge, Massachusetts 02142 (US) LI, Qingyi Cambridge, Massachusetts 02142 (US) NGOUNOU WETIE, Armand, Gatien Cambridge, Massachusetts 02142 (US) YU, Hongchuan Cambridge, Massachusetts 02142 (US) KIESMAN, William, F. Cambridge, Massachusetts 02142 (US) WANG, Guangliang Cambridge, Massachusetts 02142 (US) (74) Representative: Carpmaels & Ransford LLP  One Southampton Row London WC1B 5HA London WC1B 5HA (GB)