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EP 4 551 696 A2

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(88) Date of publication A3: 29.02.2024 (43) Date of publication: 14.05.2025 Bulletin 2025/20 (21) Application number: 23836274.3 (22) Date of filing: 06.07.2023

(51) International Patent Classification (IPC):  C12N 9/22(2006.01) C12N 15/113(2010.01) C12N 15/63(2006.01) C12N 15/10(2006.01) C07K 19/00(2006.01) C12N 15/62(2006.01) C12N 15/86(2006.01) (52) Cooperative Patent Classification (CPC): C12N 9/22; C12N 15/102; C12N 2310/20; C12N 15/902; C07K 2319/01; C07K 2319/09 (86) International application number: PCT/US2023/069709 (87) International publication number: WO 2024/011176 (11.01.2024 Gazette 2024/02)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC ME MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA Designated Validation States: KH MA MD TN (30) Priority: 08.07.2022 US 202263359359 P (71) Applicants: The Regents of the University of California Oakland, CA 94607 (US) Evercrisp Biosciences New York, NY 10169 (US)

(72) Inventors: DOUDNA, Jennifer A. Berkeley, California 94705 (US) KORTEMME, Tanja San Francisco, California 94143 (US) JAGGER, Benjamin San Francisco, California 94143 (US) KRIVACIC, Cody San Francisco, California 94143 (US) CHEN, Kai Berkeley, California 94709 (US) LIRAS, Spiros Cambridge, Massachusetts 02142 (US) RUSU, Victor Cambridge, Massachusetts 02142 (US) (74) Representative: Mewburn Ellis LLP  Aurora Building Counterslip Bristol BS1 6BX Bristol BS1 6BX (GB)