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EP 4 558 642 A1

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(43) Date of publication: 28.05.2025 Bulletin 2025/22 (21) Application number: 23843830.3 (22) Date of filing: 18.07.2023

(51) International Patent Classification (IPC):  C12Q 1/6806(2018.01) C12Q 1/6869(2018.01) (52) Cooperative Patent Classification (CPC): C12Q 1/6806   C-Sets: C12Q 1/6806, C12Q 2525/191, C12Q 2533/101, C12Q 2525/301, C12Q 2525/307, C12Q 2533/107, C12Q 2531/125; (86) International application number: PCT/US2023/070442 (87) International publication number: WO 2024/020410 (25.01.2024 Gazette 2024/04)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC ME MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA Designated Validation States: KH MA MD TN (30) Priority: 22.07.2022 US 202263391664 P (71) Applicant: Pacific Biosciences of California, Inc. Menlo Park, CA 94025 (US)	(72) Inventors: WILLIAMS, Lindsey Menlo Park, California 94025 (US) SHEE, Chandan Menlo Park, California 94025 (US) BERGHUIS, Bojan Menlo Park, California 94025 (US) STAKER, Bryan P. Menlo Park, California 94025 (US) LAKDAWALLA, Abizar Menlo Park, California 94025 (US) (74) Representative: Mewburn Ellis LLP  Aurora Building Counterslip Bristol BS1 6BX Bristol BS1 6BX (GB)