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EP 4 569 331 A1

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(43) Date of publication: 18.06.2025 Bulletin 2025/25 (21) Application number: 23758527.8 (22) Date of filing: 09.08.2023

(51) International Patent Classification (IPC):  G01N 33/68(2006.01) B82Y 15/00(2011.01) C07K 14/475(2006.01) B82Y 5/00(2011.01) C07K 14/00(2006.01) C12Q 1/6869(2018.01) (52) Cooperative Patent Classification (CPC): B82Y 5/00; C12Q 1/6869; G01N 33/6872; C07K 14/245; C07K 2319/00; B82Y 15/00   C-Sets: C12Q 1/6869, C12Q 2565/631; (86) International application number: PCT/EP2023/072113 (87) International publication number: WO 2024/033447 (15.02.2024 Gazette 2024/07)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC ME MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA Designated Validation States: KH MA MD TN (30) Priority: 09.08.2022 US 202263396539 P (71) Applicants: Oxford Nanopore Technologies PLC Oxford OX4 4DQ (GB) THE REGENTS OF THE UNIVERSITY OF CALIFORNIA Oakland, CA 94607-5200 (US)

(72) Inventors: WALLACE, Elizabeth Jayne Oxford OX4 4DQ (GB) JAYASINGHE, Lakmal Nishantha Oxford OX4 4DQ (GB) HAMBLEY, Richard George Oxford OX4 4DQ (GB) SCOTT, Alistair James Oxford OX4 4DQ (GB) MALAVIARACHCHIGE RABEL, Ranga Prabhath Oxford OX4 4DQ (GB) GRIFFITHS, Rhys Connor Oxford OX4 4DQ (GB) LECKENBY, Amber Elizabeth Oxford OX4 4DQ (GB) SINGH, Pratik Raj Oxford OX4 4DQ (GB) RIERA, Alberto Oxford OX4 4DQ (GB) DEGRADO, William F. Oakland, California 94607-5200 (US) SCHNAIDER, Lee Oakland, California 94607-5200 (US) POLIZZI, Nicholas Oakland, California 94607-5200 (US) (74) Representative: Greaves Brewster LLP  Copa House Station Road Cheddar, Somerset BS27 3AH Cheddar, Somerset BS27 3AH (GB)