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EP 4 577 647 A1

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(43) Date of publication: 02.07.2025 Bulletin 2025/27 (21) Application number: 23858091.4 (22) Date of filing: 24.08.2023

(51) International Patent Classification (IPC):  C12N 9/22(2006.01) C12N 15/11(2006.01) C07K 14/00(2006.01) C12N 15/90(2006.01) (52) Cooperative Patent Classification (CPC): C12N 9/22; C07K 14/47; C07K 2319/00; C12N 15/907; A61K 48/005; C12N 2310/20; C12N 9/78; C12Y 305/04002; C12Y 305/04001 (86) International application number: PCT/US2023/031071 (87) International publication number: WO 2024/044329 (29.02.2024 Gazette 2024/09)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC ME MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA Designated Validation States: KH MA MD TN (30) Priority: 24.08.2022 US 202263373411 P (71) Applicants: The Regents Of The University Of Michigan Ann Arbor, Michigan 48109-1280 (US) The Broad Institute, Inc. Cambridge, Massachusetts 02142 (US) President And Fellows Of Harvard College Cambridge, Massachusetts 02138 (US)

(72) Inventors: ZHANG, Yan Ann Arbor, Michigan 48109 (US) LIU, David R. Cambridge, Massachusetts 02142 (US) GAO, Xin D. Cambridge, Massachusetts 02142 (US) ZHAO, Kevin T. Cambridge, Massachusetts 02138 (US) HOU, Zhonggang Ann Arbor, Michigan 48109 (US) (74) Representative: Boult Wade Tennant LLP  Salisbury Square House 8 Salisbury Square London EC4Y 8AP London EC4Y 8AP (GB)