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EP 4 680 764 A2

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(88) Date of publication A3: 21.11.2024 (43) Date of publication: 21.01.2026 Bulletin 2026/04 (21) Application number: 24717528.4 (22) Date of filing: 15.03.2024

(51) International Patent Classification (IPC):  C12Q 1/6806(2018.01) C12Q 1/6844(2018.01) (52) Cooperative Patent Classification (CPC): C12Q 1/6806; C12Q 1/6844   C-Sets: C12Q 1/6806, C12Q 2521/319, C12Q 2521/501, C12Q 2521/507, C12Q 2521/531, C12Q 2522/101, C12Q 2523/113, C12Q 2531/125, C12Q 2533/107, C12Q 2535/122, C12Q 2543/10, C12Q 2543/101, C12Q 2563/179; C12Q 1/6844, C12Q 2521/501, C12Q 2521/507, C12Q 2522/101, C12Q 2523/113, C12Q 2531/125, C12Q 2535/122, C12Q 2543/101, C12Q 2563/179; (86) International application number: PCT/US2024/020154 (87) International publication number: WO 2024/196764 (26.09.2024 Gazette 2024/39)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC ME MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA Designated Validation States: GE KH MA MD TN (30) Priority: 17.03.2023 US 202363490865 P 17.03.2023 US 202363490868 P 17.03.2023 US 202363490871 P 17.03.2023 US 202363490873 P (71) Applicant: Bruker Spatial Biology, Inc. Billerica, Massachusetts 01821 (US)

(72) Inventors: BEECHEM, Joseph, M. Eugene, OR 97405 (US) RUFF, David Seattle, WA 98109 (US) WU, Lidan Seattle, WA 98105 (US) (74) Representative: D Young & Co LLP  3 Noble Street London EC2V 7BQ London EC2V 7BQ (GB)