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EP 4 815 964 A1

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(43) Date of publication: 07.10.2026 Bulletin 2026/41 (21) Application number: 24898737.2 (22) Date of filing: 27.11.2024

(51) International Patent Classification (IPC):  A61K 31/7105(2006.01) A61K 38/46(2006.01) C12N 15/11(2006.01) C12N 9/22(2006.01) A61K 31/711(2006.01) A61K 48/00(2006.01) C12N 15/86(2006.01) C12N 9/78(2006.01) (52) Cooperative Patent Classification (CPC): C12N 2750/14143; C12N 15/86; C12N 15/113; C12N 2310/121; C12N 15/111; C12N 2310/20 (86) International application number: PCT/US2024/057689 (87) International publication number: WO 2025/117698 (05.06.2025 Gazette 2025/23)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC ME MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA Designated Validation States: GE KH MA MD TN (30) Priority: 01.12.2023 US 202363605049 P (71) Applicants: Massachusetts Institute of Technology Cambridge, MA 02139-4301 (US) The Brigham & Women's Hospital, Inc. Boston, Massachusetts 02115 (US) Beth Israel Deaconess Medical Center Boston, MA 02215 (US)

(72) Inventors: ABUDAYYEH, Omar Cambridge, MA 02141 (US) GOOTENBERG, Jonathan Cambridge, MA 02139 (US) SCHMITT-ULMS, Cian Cambridge, MA 02139 (US) KAYABOLEN, Alisan Cambridge, MA 02139 (US) (74) Representative: Hoffmann Eitle  Patent- und Rechtsanwälte PartmbB Arabellastraße 30 81925 München 81925 München (DE)