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(11)

EP 4 816 547 A1

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(43) Date of publication: 07.10.2026 Bulletin 2026/41 (21) Application number: 24828238.6 (22) Date of filing: 27.11.2024

(51) International Patent Classification (IPC):  C12Q 1/6806(2018.01) (52) Cooperative Patent Classification (CPC): C12Q 1/6855   C-Sets: C12Q 1/6855, C12Q 2525/155, C12Q 2535/122, C12Q 2563/179, C12Q 2565/50; (86) International application number: PCT/US2024/057746 (87) International publication number: WO 2025/117738 (05.06.2025 Gazette 2025/23)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC ME MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA Designated Validation States: GE KH MA MD TN (30) Priority: 28.11.2023 US 202363603245 P (71) Applicant: Illumina, Inc. San Diego, CA 92122 (US)

(72) Inventors: FRASER, Louise Cambridge Limited 19 Granta Park, Great Abington Cambridge CB21 6DF (GB) MORRELL, Natalie Cambridge Limited 19 Granta Park, Great Abington Cambridge CB21 6DF (GB) BORBA, Ana Rita Cambridge Limited 19 Granta Park, Great Abington Cambridge CB21 6DF (GB) GORMLEY, Niall Anthony Cambridge Limited 19 Granta Park, Great Abington Cambridge CB21 6DF (GB) LEE, Ming-Hsiang San Diego, California 92122 (US) RAHMANIAN, Sorena San Diego, California 92122 (US) LOCOCO, Jennifer San Diego, California 92122 (US) CHOU, Danny San Diego, California 92122 (US) (74) Representative: Williams, Andrea  Marks & Clerk LLP 62-68 Hills Road Cambridge CB2 1LA Cambridge CB2 1LA (GB)