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(11)

EP 4 823 593 A1

(12)

(43) Date of publication: 21.10.2026 Bulletin 2026/43 (21) Application number: 24834551.4 (22) Date of filing: 15.12.2024

(51) International Patent Classification (IPC):  A61K 38/00(2006.01) (52) Cooperative Patent Classification (CPC): A61K 38/00; C12N 2740/10022; C12N 2740/10023; C07K 14/43581; C07K 14/705; C07K 2319/735; C07K 2319/85; C07K 2319/80; C07K 2319/10; C07K 2319/01; C07K 14/4716; C12Y 301/01004; C12N 15/85 (86) International application number: PCT/US2024/060268 (87) International publication number: WO 2025/129158 (19.06.2025 Gazette 2025/25)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC ME MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA Designated Validation States: GE KH MA MD TN (30) Priority: 15.12.2023 US 202363610858 P 21.06.2024 US 202463662813 P (71) Applicants: The Broad Institute, Inc. Cambridge, MA 02142 (US) MASSACHUSETTS INSTITUTE OF TECHNOLOGY Cambridge, MA 02139 (US)

(72) Inventors: ZHANG, Feng Cambridge, Massachusetts 02142 (US) SEGEL, Michael Cambridge, Massachusetts 02139 (US) LASH, Blake Cambridge, Massachusetts 02139 (US) STREBINGER, Daniel Cambridge, Massachusetts 02142 (US) (74) Representative: V.O.  P.O. Box 87930 2508 DH Den Haag 2508 DH Den Haag (NL)