(19)

(11)

EP 4 824 242 A1

(12)

(43) Date of publication: 21.10.2026 Bulletin 2026/43 (21) Application number: 24834189.3 (22) Date of filing: 16.12.2024

(51) International Patent Classification (IPC):  C12N 9/12(2006.01) C12N 15/00(2006.01) C12N 15/52(2006.01) (52) Cooperative Patent Classification (CPC): C12N 9/1252; C12N 15/52; C12Y 207/07007; C12N 15/00 (86) International application number: PCT/US2024/060411 (87) International publication number: WO 2025/129195 (19.06.2025 Gazette 2025/25)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC ME MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA Designated Validation States: GE KH MA MD TN (30) Priority: 15.12.2023 US 202363610498 P (71) Applicant: New England Biolabs, Inc. Ipswich, MA 01938 (US)

(72) Inventors: CROSBY, Heidi Ipswich, MA 01938 (US) MINSKY, Burcu, Baykal Ipswich, MA 01938 (US) ONG, Jennifer Ipswich, MA 01938 (US) PATTON, Gregory Ipswich, MA 01938 (US) TANNER, Nathan Ipswich, MA 01938 (US) MA, Dong Ipswich, MA 01938 (US) JOHNSON, Sean, R. Ipswich, MA 01938 (US) GRAY, Andrew Ipswich, MA 01938 (US) (74) Representative: Mathys & Squire  32 London Bridge Street The Shard London SE1 9SG London SE1 9SG (GB)