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(11)

EP 4 824 801 A2

(12)

(88) Date of publication A3: 31.07.2025 (43) Date of publication: 21.10.2026 Bulletin 2026/43 (21) Application number: 24841548.1 (22) Date of filing: 13.12.2024

(51) International Patent Classification (IPC):  G16B 30/10(2019.01) C12Q 1/6806(2018.01) G16B 40/00(2019.01) (52) Cooperative Patent Classification (CPC): C12Q 1/6806   C-Sets: C12Q 1/6806, C12Q 2565/537, C12Q 2563/173, C12Q 2535/122; (86) International application number: PCT/US2024/060135 (87) International publication number: WO 2025/129074 (19.06.2025 Gazette 2025/25)

(84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC ME MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA Designated Validation States: GE KH MA MD TN (30) Priority: 14.12.2023 US 202363610279 P 15.12.2023 US 202363611026 P 01.11.2024 US 202463715419 P 04.11.2024 US 202463715950 P (71) Applicant: Illumina, Inc. San Diego, CA 92122 (US)

(72) Inventors: ARTIOLI, Gianluca Andrea San Diego, California 92122 (US) BASUKI, Johan Sebastian San Diego, California 92122 (US) FRASER, Louise Jane San Diego, California 92122 (US) GORMLEY, Niall Anthony San Diego, California 92122 (US) MARTINS VITORIANO, Maria Ines San Diego, California 92122 (US) MORRELL, Natalie San Diego, California 92122 (US) RICOULT, Sebastien Georg Gabriel San Diego, California 92122 (US) SARKAR, Krishnarjun San Diego, California 92122 (US) VON HATTEN, Xavier San Diego, California 92122 (US) WEIR, Jacqueline C. San Diego, California 92122 (US) BEKRITSKY, Mitchell San Diego, California 92122 (US) ANDREWS, Daniel San Diego, California 92122 (US) MUSGRAVE-BROWN, Esther San Diego, California 92122 (US) THOMSON, Vicki San Diego, California 92122 (US) (74) Representative: Forresters IP LLP  Skygarden Erika-Mann-Straße 11 80636 München 80636 München (DE)