Diagnostic kit for blood pool imaging

Description

BACKGROUND OF THE INVENTION

[0001] One of the methods employed in the past to image the blood pool for diagnostic purposes involves the intravenous administration of 99m Tc serum albumin which is available commercially and is a sterile pyrogen-free solution of albumin labelled with Technetium 99m having an activity of greater than 100 microcuries/ml.

[0002] In utilizing this method of blood pool imaging, it is important to predose the patient immediately prior to the attempted visualization of the blood pool, since the 99m Tc serum albumin is very rapidly lost from the blood stream by exchange in the kidney.

[0003] Another method used in the past for imaging blood pools is the in vitro labelling of red blood cells (outside the host animal) followed by reinjection of the cells into the vascular system of the selected animal. By this method, it is possible to visualize both heart blcod pools plus major peripheral vessels up to 3 hours after injection of the labelled cells. As can be seen, this is a complicated procedure, and therefore is a more time- consuming and expensive method.

[0004] Still another procedure for imaging blood involves the injection of stannous pyrophosphate followed by injection of 99m Tc-pertechnetate. This technique is reported as successful in producing satisfactory imaging of blood pools.

[0005] In accordance with the present invention, there is provided a diagnostic kit suitable for the radioactive labelling of red blood cells in vivo, thus making possible the imaging of blood pools within the circulatory system of the patient being examined. As an integral part of the diagnostic kit of the present invention, there is also provided a novel chemical composition comprising a lyophilized mixture of a glucoheptonate salt and a pharmaceutically acceptable stannous salt in the ratio of 25 parts by weight of glucoheptonate salt to 3 parts by weight of stannous salt measured as calcium glucoheptonate and stannous chloride dihydrate or approximately 22.9 parts of glucoheptonate ion to 1.32 parts of tin calculated as available stannous ion.

[0006] An important feature of the present invention is the provision of an individual diagnostic kit containing a non-toxic stannous salt capable of supplying an amount of stannous ion equivalent to no more than 4.0 mg and preferably at least 2.5 mg of stannous chloride dihydrate. Less than 2.5 mg of the stannous chloride does not provide sufficient stannous ion to effect a satisfactory degree of labelling of red blood cells. Because of the known toxicity of stannous salts, no more than 4 mg of stannous chloride dihydrate or a stannous salt containing an equivalent amount of stannous ion should be incorporated in an individual dosage kit.

[0007] The present invention also includes the process for the preparation of the novel chemical composition and the diagnostic method for imagine blood pools in patients suspected of having abnormalities in the circulatory system.

[0008] In accordance with the process of the present invention, a sterile solution of a non-toxic, pharmaceutically acceptable salt of glucoheptonic acid, e.g., calcium glucoheptonate, is mixed with a non-toxic stannous salt in a ratio of 25 parts by weight of glucoheptonate salt to 3 parts by weight of stannous salt, calculated as calcium glucoheptonate and stannous chloride dihydrate.

[0009] The solution is adjusted to a neutral pH 6-8, subdivided, and lyophilized to produce individual vials containing a dry, sterile mixture comprising 25 mg. calcium glucoheptonate and 3 mg. stannous chloride·dihydrate.

[0010] In utilizing the kit of the present invention for imaging the blood pools of a patient to diagnose abnormalities.in the cardiovascular system, a vial containing the dry, sterile mixture of calcium glucoheptonate and stannous chloride dihydrate is reconstituted by mixing with 2-8 ml. of a USP saline solution. The reconstituted solution is then used for injection of the patient to be examined. After a period of 30 minutes, a second injection of 2-8 ml. of a sterile saline solution of sodium pertechnetate is made. Following the injection of sodium pertechnetate, it is possible, after waiting from 30 seconds to 2 minutes, to image the blood pools in the patient being.examined. This "in vivo" labeling of the red blood cells is exceptionally stable and approximately 95% of the radioactivity is retained by the red blood cells for at least 6 hours following injection. This simple procedure avoids the instability of the human serum albumin/99m Tc injection of the prior art as well as the expense and inconvenience of the in vitro labeling of the red blood cells noted as an alternate prior art method.

EXAMPLE 1

Blood Pool Imaging Kit

[0011] A solution is prepared under sterile conditions with 25 g. of calcium glucoheptonate in sterile, pyrogen-free water which has been purged with nitrogen. The solution of calcium glucoheptonate is stored and purged under nitrogen. In a separate container, 3 g. of stannous chloride dihydrate is dissolved in 1 ml. of hydrochloric acid, and the resulting solution diluted with nitrogen-purged, sterile water to a volume of 10 ml. The stannous chloride dihydrate solution is then added to the calcium glucoheptonate with stirring and flushing with nitrogen. The solution is mixed thoroughly, and the pH is adjusted to neutrality with a solution of sterile IN sodium hydroxide solution. The volume is then adjusted to 2000 ml. with sterile, nitrogen-purged water and subdivided into vials, each containing 2 ml. of the solution. The vials are then lyophilized and sealed under nitrogen. Each. vial contains 25 mg. of calcium glucoheptonate and 3 mg. of stannous chloride dihydrate.

EXAMPLE 2

Method of Using Blood Pool Imaging Kit

[0012] A solution of sodium chloride for injection USP (2 ml.) is added to a vial containing a lyophilized mixture of 3 mg. of stannous chloride dihydrate and 25.0 mg. calcium glucoheptonate. The resulting solution is used for the intravenous injection of patients for the purpose of imaging the blood pools for diagnostic purposes. The amount of solution used is based on the weight of the patient and sufficient volume is used so that 30 mcg./kg. of stannous ion, measured as stannous chloride dihydrate, is injected. It is recommended that no more than the contents of one vial be administered to any patient. After waiting a period of 30 minutes, a sterile saline solution of sodium ^pertech- netate_-Tc99m (2-20 mCi) is injected. This tags the red blood cells and permits imaging of the blood pools of the patient being examined almost immediately (from 30 seconds to 2 minutes).

Claims

1 A diagnostic kit used for blood pool imaging comprising a sterile sealed vial containing a lyophilized mixture of a non-toxic pharmaceutically acceptable salt of glucoheptonic acid and a non-toxic pharmaceutically acceptable stannous salt in a ratio of 22.9 parts by weight of glucoheptonate ion to 1.32 parts of stannous ion, provided that the individual dose of stannous ion does not exceed 4 mg, calculated as stannous chloride dihydrate.

2 A kit according to claim 1 in which the salt of glucoheptonic acid is calcium glucoheptonate and the stannous salt is stannous chloride dihydrate.

3 A kit according to claim 2 in which the lyophilized mixture comprises from 2.5-4 mg of stannous chloride dihydrate and 25 mg of calcium glucoheptonate.

4 A kit according to claim 3 that comprises a lyophilized mixture of 3 mg of stannous chloride dihydrate and 25 mg of calcium glucoheptonate.

5 A process for the preparation of a blood pool imaging kit that comprises preparing a sterile aqueous solution of a water-soluble non-toxic pharmaceutically acceptable salt of glucoheptonic acid and a non-toxic pharmaceutically acceptable stannous salt, subdividing the said solution into individual dose vials under nitrogen, and lyophilizing and sealing the said vials under nitrogen, in which the individual dose of stannous ion does not exceed 4 mg, calculated as stannous chloride dihydrate, and there is 22.9 parts by weight of glucoheptonate ion to 1.32 parts of stannous ion.

6 A process according to claim 5 in which the glucoheptonate salt is calcium glucoheptonate and the stannous salt is stannous chloride dihydrate.

7 A process according to claim 6 in which the individual dose vials contain from 2.5-4 mg of stannous salt calculated as stannous chloride dihydrate and 25 mg of calcium glucoheptonate.

8 A process according to claim 7 in which the individual dose vials contain 3 mg of stannous salt, calculated as stannous chloride dihydrate, and 25 mg of calcium glucoheptonate.

9 A method of imaging blood pools in patients suspected of having cardiovascular abnormalities which comprises the steps of

(1) intravenous injection of a solution comprising a water soluble, non-toxic, pharmaceutically acceptable salt of ketoglucoheptcnic acid and a water soluble non-toxic stannous salt in the ratio set forth in claim 1, provided that the individual dose of stannous ion does not exceed 4 mg, calculated as stannous chloride dihydrate,

(2) waiting a period of 30 minutes; and

(3) intravenous injection of a sterile saline solution containing from 2-20 mCi of sodium pertechnetate-Tc99m.

10 A method according to claim 9 in which the glucoheptonate salt used is calcium glucoheptonate and the stannous salt is stannous chloride dihydrate.