FIELD OF THE INVETION
[0001] The present invention relates to remedies for AIDS (Acquired Immunodeficiency Syndrome).
Background Art
[0002] AIDS is a disease caused by HIV (Human Immunodeficiency Virus) infection, and the
number of patients suffering AIDS has markedly increased in recent years. In therapy
for AIDS, there have been used nucleoside-type anti-HIV agents such as Zidovudine
(Azidothymidine, AZT) and Didanosine (ddI).
[0003] However, these conventional anti-HIV agents do not provide sufficient therapeutic
effect and thus development of new AIDS therapeutic means is demanded.
DISCLOSURE OF THE INVENTION
[0004] The present inventors have conducted careful studies in an attempt to improve the
therapeutic effect of nucleoside-type anti-HIV agents on AIDS patients. They have
found that the combined use of a hot water extract of tubercle bacillus and a nucleoside-type
anti-HIV agent dramatically improves the mortality of AIDS patients as compared with
the case in which the anti-HIV agent alone is administered, leading to completion
of the invention.
[0005] Accordingly, the present invention provides remedies for AIDS, which comprise as
active ingredients (a) a composition containing as the primary component a polysaccharide
derived from a hot water extract of tubercle bacillus and (b) a nucleoside-type anti-HIV
agent.
[0006] The present invention also provides enhancer drugs for improving the therapeutic
effect of a nucleoside-type anti-HIV agent on AIDS, which comprise as an active ingredient
(a) a composition containing as the primary component a polysaccharide derived from
a hot water extract of tubercle bacillus.
[0007] The present invention further provides use of (a) a composition containing as the
primary component a polysaccharide derived from a hot water extract of tubercle bacillus,
for the manufacture of enhancer drugs which improve therapeutic effect of a nucleoside-type
anti-HIV agent on AIDS.
[0008] The present invention still further provides use of (a) a composition containing
as the primary ingredient a polysaccharide derived from a hot water extract of tubercle
bacillus and (b) a nucleoside-type anti-HIV agent, for the manufacture of remedies
for AIDS.
[0009] The present invention yet further provides a method for treating AIDS, which comprises
administering to an HIV-infected patient (a) a composition containing as the primary
component a polysaccharide derived from a hot water extract of tubercle bacillus and
(b) a nucleoside-type anti-HIV agent.
BRIEF DESCRIPTION OF DRAWINGS
[0010]
Fig. 1 is a graph showing the chronological change of the survival rate of mice suffering
murine AIDS (MAIDS).
BEST MODE FOR CARRYING OUT THE INVENTION
[0011] The composition (a); i.e., a composition containing as the primary component a polysaccharide
derived from a hot water extract of tubercle bacillus, which is used in the present
invention is known to have excellent recovery action against reduction of white blood
cells caused by radiotherapy for cancers (see among others "The Clinical Report (Basic
and Clinical Report)," 24(4), 1973 (1990) and "Nippon Igaku Hoshasen Gakkai Zasshi,"
50(8), 993(1990)).
[0012] However, the effect of this composition (a) on AIDS is still unknown.
[0013] The composition (a) contains a polysaccharide as the primary component. Preferably,
the composition (a) contains a polysaccharide whose primary constituents are arabinose,
mannose, and glucose, and a small amount of nucleic acid. The polysaccharide preferably
has a molecular weight ranging from 5 x 10
2 to 5 x 10
4 as measured by the gel filtration method. The nucleic acid content of composition
(a) is preferably 0.05-0.3 wt.%. The composition may contain 1-5 wt.% of protein.
Preferably, the mannose content of the polysaccharide is 10-72 wt.%, the arabinose
content 3-30 wt.%, and the glucose content 5-30 wt.%, and particularly preferred are
40-50 wt.% mannose, 15-25 wt.% arabinose, and 5-15 wt.% glucose.
[0014] The composition (a) is obtained by purifying a hot-water extract of tubercle bacillus.
In more detail, composition (a) is obtained by subjecting cells of tubercle bacillus
to extraction with hot water, and the extract is then subjected to protein removal
treatment and to treatment for removing polysaccharides having molecular weights of
10
5 or more. The tubercle bacillus usable in the present invention can be optionally
selected from both of human type tubercle bacillus and animal type tubercle bacillus,
but human type tubercle bacillus is preferred. Exemplified as the animal type tubercle
bacillus are
Mycobacterium (M) bovis, M. avium, M. microti, M. kansaii, M. marium and
M. intracellulae. Exemplified as the human type tubercle bacillus are an Aoyama B strain, H37Rv strain
and H37Ra strain, particularly the Aoyama B strain being preferred. Among the aforementioned
steps, extraction with hot water is preferably performed with 80-120°C water. Exemplified
as the extraction water are water, a saline solution and a NaOH solution, particularly
water being preferred. The protein removal treatment is preferably performed by causing
proteins to precipitate by use of a protein precipitant such as sulfosalicylic acid,
trichloroacetic acid, or phosphotungstic acid; and subsequently collecting the supernatant.
The treatment to remove polysaccharides having molecular weights of 10
5 or more is preferably performed by causing polysaccharides having high molecular
weights to precipitate by use of ethanol, methanol, or acetone in a suitable amount;
and subsequently collecting the supernatant.
[0015] The component (b); a nucleoside-type anti-HIV agent, is not particularly limited.
Preferably exemplified as component (b) are one or more members selected from among
Zidovudine (Azidothymidine, AZT), Didanosine (ddI), Zalcitabin (ddC), Stavudine (d4T)
and Lamivudime (3TC). Azidothymidine is particularly preferred.
[0016] In the present invention, the composition (a) is preferably administered to a subject
in need thereof by injection, particularly preferably subcutaneous injection. The
nucleoside-type anti-HIV agent (b) is preferably administered to a subject in need
thereof perorally or by injection, depending on the type of the agent. Examples of
the forms of the agent for oral administration include granules, tablets, capsules,
and liquids.
[0017] The dose of the composition (a) is preferably 2-200 µg per day, more preferably 20-100
µg per day, as calculated in terms of the saccharide content of arabinose. The dose
of the nucleoside anti-HIV agent (b), which differs in accordance with the type of
drug and administration route, is preferably 50-1500 mg per day, more preferably 200-1000
mg per day, in the case of oral administration.
[0018] When the composition (a) and the nucleoside-type anti-HIV agent (b) are administered
to a subject in need thereof, they are preferably respectively prepared into pharmaceutical
compositions suitable for the aforementioned administration routes by incorporating
thereto a generally employed, pharmaceutically acceptable carrier. Examples of carriers
useful for the preparation of pharmaceutical compositions include vehicles, binders,
lubricants, disintegrants, coating agents, emulsifiers, suspensions, solvents, stabilizers,
absorption aids, water for injection use, and tonicity agents.
EXAMPLES
[0019] The present invention will next be described by way of examples, which should not
be construed as limiting the invention thereto.
Referential Example 1 (Preparation of composition (a)):
[0020] Mycobacterium tuberculosis strain Aoyama B which had been lyophilized and stored at -20° was subjected to seed
culture at 37 ± 1°C in a Sauton-potato medium
(1). The resultant cells were inoculated to a production medium
(2) and incubated for 5-7 weeks at 37 ± 1°C. The harvested cells were washed with water
for injection use, and to the wet cells was added water for injection use in an amount
20 times that of the weight of the wet cells. The mixture was heated at 100°C for
120 minutes, to thereby obtain an extract. The extract was filtered by use of a 0.45
µm-membrane filter and concentrated under reduced pressure so that the saccharide
content (converted to D-arabinose by the phenol-sulfuric acid method) fell within
the range of 4.0-6.0 mg/ml, to thereby obtain a concentrate. Subsequently, in order
to remove proteins, one (w/v)% sulfosalicylic acid was added to the concentrate. The
mixture was allowed to stand for 15-20 minutes at a temperature of not higher than
10°C. Precipitates were removed by centrifugal separation (10°C or lower temperature,
1,150 x G, 10 minutes), to thereby recover the supernatant. The protein concentration
of the supernatant was not more than 0.30 mg/ml (Lowry method, calculated as tyrosine).
The supernatant was further processed to remove sulfosalicylic acid until the content
of sulfosalicylic acid was below the detection limit (10 ppm or less, colorimetric
method using ferric chloride solution). The resultant solution was concentrated under
reduced pressure so that the saccharide content fell within the range of 1.8-2.2 mg/ml,
and the concentrate was combined with sodium chloride (0.9 (w/v)%) and cold ethanol
whose volume is the same as that of the concentrate. The mixture was allowed to stand
for not less than 40 hours at a temperature of not higher than 10°C, and then the
precipitates polysaccharide of high molecular weight region were removed by centrifugal
separation (10°C or lower temperature, 2,040 x G, 10 minutes). Subsequently, the supernatant
was combined with four times the amount of cold ethanol, and the mixture was allowed
to stand for not less than 40 hours at a temperature of not higher than 10°C, to thereby
recover precipitates. The precipitates were dissolved in water for injection use and
after the saccharide content was adjusted to 1.8-2.2 mg/ml, the solution was subjected
to filtration by use of a 0.45 µm membrane filter and to sterilization in an autoclave
(121°C, 20 minutes), to thereby prepare a composition (a) solution.
(1) Sauton-potato medium
[0021] Washed potato slices were soaked in a Sauton medium, followed by sterilization for
15 minutes at 115°C. The resultant material was used as a Sauton-potato medium.
Sauton Medium |
L-asparagine (monohydrate) |
4.0 g |
Citric acid (monohydrate) |
2.0 g |
Magnesium sulfate (heptahydrate) |
0.5 g |
Potassium monohydrogenphosphate (anhydrate) |
0.5 g |
Ammonium iron citrate |
0.05 g |
Glycerol |
60 ml |
[0022] The above ingredients were dissolved in water to make a total volume of 1,000 ml.
By use of sodium hydroxide solution, pH was adjusted to 7.0-7.3.
(2) Production medium
[0023]
L-asparagine (monohydrate) |
4.0 g |
Citric acid (monohydrate) |
2.0 g |
Magnesium sulfate (heptahydrate) |
0.5 g |
Potassium monohydrogenphosphate (anhydrate) |
0.5 g |
Ammonium iron citrate |
0.05 g |
Glycerol |
60 ml |
[0024] The above ingredients were dissolved in water to make a total volume of 1,000 ml,
followed by sterilization in an autoclave (121°C, 20 minutes). By use of sodium hydroxide
solution, pH was adjusted to 7.0-7.3.
[0025] The physicochemical properties of the composition (a) solution were as follows.
(1) Appearance: |
Pale yellow clear liquid |
(2) pH: |
4.50-5.30 |
(3) Protein content: |
3.5 wt.% (as an amino acid) in a freeze-dried product |
(4) Nucleic acid content: |
0.1 wt.% in a freeze-dried product |
(5) Constituent monosaccharides of polysaccharide: |
Mannose 43.4 wt.%, |
Arabinose 18.2 wt.%, and |
Glucose 10.4 wt.%. |
[0026] Methods for determining the constituent monosaccharides of polysaccharide: The polysaccharide
was hydrolyzed with 2N trifluoroacetic acid for two hours at 100°C, and then subjected
to liquid chromatography making use of 2-cyanoacetamide fluoroscein derivative (S.
Honda,
et al., Anal. Chem., 52, 1079 (1980)).
Test Example:
[0027] In accordance with the method described in Antimicrobial Agents and Chemotherapy,
Apr. 1990, p.605-609, culture supernatant (0.1 ml) of SC-1 cells infected with LP-BM5
murine leukemia virus was intraperitoneally administered to each of a group of male
C57BL/6 mice, to thereby create murine AIDS (MAIDS) mice. AZT and/or composition (a)
(a solution obtained in Referential Example 1) were/was administered to the MAIDS
mice from the day following the viral infection. AZT was orally administered to each
mouse at a dose of 10 mg/kg/day every four days. In the case of composition (a), the
solution obtained in Referential Example 1 was intraperitoneally administered to each
mouse at a dose of 10 mg/kg/day (calculated as the saccharide content) every two days.
[0028] After administration of AZT and/or composition(a), the MAIDS mice were bred for 145
days under observation. The survival rate was determined, and the therapeutic effects
of AZT and composition (a) were evaluated. The survival rates after 130 days are summarized
in Table 1 and the chronological changes of the survival rates are shown in Fig. 1.
Table 1
Group |
No. of mice |
Alive |
Dead |
Suvival rate (%) |
Control |
22 |
8 |
14 |
36 |
Comp. (a) |
10 |
3 |
7 |
30 |
AZT |
10 |
6 |
4 |
60 |
Comp. (a) + AZT |
10 |
8 |
2 |
80* |
Statistical significance between the control group and the treated group in a survival
rate was evaluated at day 130 after the viral infection. |
*: p=0.027, compared with control (Fisher's exact method). |
[0029] As shown in Table 1 and Fig. 1, mortality of MAIDS mice administered with AZT and
composition (a) was clearly improved as compared with that of MAIDS mice administered
with AZT alone.
INDUSTRIAL APPLICABILITY
[0030] As described above, the therapeutic method of the present invention provides remarkably
improved therapeutic effect on AIDS patients as compared with the case in which a
nucleoside-type anti-HIV agent alone is administered.
1. Remedies for AIDS, which comprise as active ingredients (a) a composition containing
as the primary component a polysaccharide derived from a hot water extract of human-type
tubercle bacillus and (b) a nucleoside-type anti-HIV agent.
2. The remedies according to Claim 1, wherein the composition (a) comprises a polysaccharide
constituted by arabinose, mannose and glucose as primary constituent monosaccharides,
and nucleic acid.
3. The remedies according to Claim 1, wherein the composition (a) comprises a polysaccharide
having a molecular weight of 5 x 102 - 5 x 104 (as determined by gel filtration) and constituted by arabinose, mannose and glucose
as primary constituent monosaccharides, and nucleic acid.
4. The remedies according to any of Claims 1 to 3, wherein the tubercle bacillus is human-type
tubercle bacillus.
5. The remedies according to any of Claims 1 to 4, wherein the tubercle bacillus is an
Aoyama B strain.
6. The remedies according to any of Claims 1 to 5, wherein the nucleoside-type anti-HIV
agent (b) is selected from the group consisting of Zidovudine (Azidothymidine, AZT),
Didanosine (ddI), Zalcitabin (ddC), Stavudine (d4T) and Lamivudime (3TC).
7. Enhancer drugs for improving the therapeutic effect of a nucleoside-type anti-HIV
agent on AIDS, which comprises as active ingredients (a) a composition containing
as the primary component a polysaccharide derived from a hot water extract of tubercle.
8. Use of (a) a composition containing as the primary component a polysaccharide derived
from a hot water extract of tubercle bacillus and (b) a nucleoside-type anti-HIV agent,
for the manufacture of remedies for AIDS.
9. The use according to Claim 8, wherein the composition (a) comprises a polysaccharide
constituted by arabinose, mannose and glucose as primary constituent monosaccharides,
and nucleic acid.
10. The use according to Claim 8, wherein the composition (a) comprises a polysaccharide
having a molecular weight of 5 x 102 - 5 x 104 (as determined by gel filtration) and constituted by arabinose, mannose and glucose
as primary constituent monosaccharides, and nucleic acid.
11. The use according to any of Claims 8 to 10, wherein the tubercle bacillus is human-type
tubercle bacillus.
12. The use according to any of Claims 8 to 11, wherein the tubercle bacillus is an Aoyama
B strain.
13. The use according to any of Claims 8 to 12, wherein the nucleoside-type anti-HIV agent
(b) is selected from the group consisting of Zidovudine (Azidothymidine, AZT), Didanosine
(ddI), Zalcitabin (ddC), Stavudine (d4T) and Lamivudime (3TC).
14. Use of (a) a composition containing as the primary component a polysaccharide derived
from a hot water extract of human-type tubercle bacillus, for the manufacture of enhancer
drugs for improving the therapeutic effect of a nucleoside-type anti-HIV agent on
AIDS.
15. A method for treating AIDS, which method comprises administering to an HIV-infected
patient (a) a composition containing as the primary component a polysaccharide derived
from a hot water extract of human-type tubercle bacillus and (b) a nucleoside-type
anti-HIV agent.
16. The method according to Claim 15, wherein the composition (a) comprises a polysaccharide
constituted by arabinose, mannose and glucose as primary constituent monosaccharides,
and nucleic acid.
17. The method according to Claim 15, wherein the composition (a) comprises a polysaccharide
having a molecular weight of 5 x 102 - 5 x 104 (as determined by gel filtration) and constituted by arabinose, mannose and glucose
as primary constituent monosaccharides, and nucleic acid.
18. The method according to any of Claims 15 to 17, wherein the tubercle bacillus is human-type
tubercle bacillus.
19. The method according to any of Claims 15 to 17, wherein the tubercle bacillus is an
Aoyama B strain.
20. The method according to any of Claims 15 to 19, wherein the nucleoside-type anti-HIV
agent (b) is selected from the group consisting of Zidovudine (Azidothymidine, AZT),
Didanosine (ddI), Zalcitabin (ddC), Stavudine (d4T) and Lamivudime (3TC).