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(11) | EP 1 291 440 A1 |
(12) | EUROPEAN PATENT APPLICATION |
published in accordance with Art. 158(3) EPC |
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(54) | KIT AND METHOD FOR DETERMINING HLA TYPE |
(57) HLA genotype of a test specimen is determined by hybridization of a nucleic acid
sequence derived from the test specimen by using a substrate on which oligonucleotides
of 10-24 nucleotide length derived from sequences of a group of genes belonging to
HLA class I or class II antigen on a human genome and including polymorphism of gene
as alloantigens in the sequences are immobilized through covalent bonds. There are
provided a typing kit and typing method that are suitable for processing of a large
number of specimens and enable high accuracy typing by one test. |
Technical Field
Background Art
1. Human leukocyte antigen (HLA) types and importance of histocompatibility
2. Genes involved in HLA typing and polymorphism
3. Means for HLA typing
4. Importance of high accuracy HLA typing
Disclosure of the Invention
the aforementioned typing kit, wherein HLA-DR is an antigen derived from any of DRA, DRB1, DRB3, DRB4 and DRB5 gene loci or a pseudogene; and
the aforementioned typing kit, wherein HLA-DP is an antigen derived from any of DPA1, DPA2, DPB1 and DPB2 gene loci or a pseudogene.
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 404 and 405 for amplifying DQB1;
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 404 and 406 for amplifying DQB1;
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 407 and 409 for amplifying DQB1;
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 408 and 409 for amplifying DQB1;
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 410 and 412 for amplifying DQB1;
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 411 and 412 for amplifying DQB1;
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 413 and 417 for amplifying DRB1;
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 414 and 417 for amplifying DRB1;
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 415 and 417 for amplifying DRB1; and
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 416 and 417 for amplifying DRB1.
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 418 and 420 for amplifying DQA1;
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 419 and 420 for amplifying DQA1;
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 421 and 422 for amplifying DQB1;
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 423 and 424 for amplifying DRA1;
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 425 and 428 for amplifying DRB1;
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 426 and 428 for amplifying DRB1;
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 427 and 428 for amplifying DRB1;
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 429 and 431 for amplifying DRB3;
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 430 and 431 for amplifying DRB3;
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 432 and 433 for amplifying DRB4;
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 434 and 435 for amplifying DRB4;
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 436 and 437 for amplifying DRB5;
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 438 and 439 for amplifying DRB6;
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 438 and 440 for amplifying DRB6;
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 441 and 442 for amplifying DRB7;
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 443 and 444 for amplifying DPA1;
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 445 and 446 for amplifying DPB1; and
a pair of oligonucleotides having nucleotide sequences of SEQ ID NOS: 445 and 447 for amplifying DPB1.
<1> Synthetic oligonucleotide for use in the present invention
<2> Preparation of substrate for use in present invention
<3> Arrangement of oligonucleotides on substrate
<4> Preparation of nucleic acid from test sample and preparation of nucleic acid probe
<5> Hybridization of oligonucleotides on substrate and nucleic acid target
Correspondence of DQA1 types and high accuracy typing capture oligos | |
Type | Corresponding capture oligos |
DQA1*0101 | 1,4,7,10,12,14,16,18,22,24,27,31,35,39,42,47,51,53 |
DQA1*01021 | 1,4,7,10,12,14,16,22,24,27,31,35,39,42,47,51,53 |
DQA1*01022 | 1,4,7,10,12,14,16,20,22,24,2731,35,39,42,47,51,53 |
DQA1*0103 | 2,4,7,10,15,20,22,26,27,31,35,39,42,47,51,53 |
DQA1*0104 | 1,4,7,10,12,14,16,18,22,24,27,31,35,39,42,47,51,53 |
DQA1*0105 | 1,4,7,10,12,14,16,18,22,24,27,31,35,39,42,47,51,53 |
DQA1*0201 | 1,5,8,10,15,19,23,24,28,32,37,39,42,47,52,53 |
DQA1*03011 | 2,5,9,13,17,19,23,24,29,33,37,41,43,48,49,52,53 |
DQA1*0302 | 2,5,9,13,17,19,23,24,29,33,36,41,43,49,52,53 |
DQA1*0303 | 1,5,9,12,17,19,23,24,29,33,36,41,43,49,52,53 |
DQA1*0401 | 2,6,8,10,12,14,16,21,22,25,30,34,38,41,44,48,52,54 |
DQA1*05011 | 3,6,8,10,12,14,16,21,22,25,30,34,38,41,45,50,52,53 |
DQA1*05012 | 1,6,8,10,12,14,16,20,22,25,30,34,38,41,45,50,52,53 |
DQA1*05013 | 3,6,8,10,12,14,16,20,22,25,30,34,38,41,45,50,52,53 |
DQA1*0502 | 8,10,12,14,16,20,22,25,30,34,38,40,45,50,52,53 |
DQA1*0503 | 3,6,8,10,12,14,16,20,22,25,30,34,38,41,45,50,52,53 |
DQA1*0504 | 3,6,8,11,14,16,20,22,25,30,34,38,41,45,50,52,53 |
DQA1*06011 | 2,6,8,10,15,21,22,25,30,34,38,41,46,48,52,53 |
DQA1*06012 | 8,10,15,21,22,25,30,34,38,41,46,48,52,53 |
Correspondence of DQB types and low accuracy typing capture oligos | |
Type | Corresponding capture oligos |
DQB1*0501 | 56 |
DQB1*0502 | 56 |
DQB1*05031 | 56 |
DQB1*06011 | 55 |
DQB1*06013 | 55 |
DQB1*0602 | 55 |
DQB1*0603 | 55 |
DQB1*0604 | 56 |
DQB1*06051 | 56 |
DQB1*0609 | 56 |
DQB1*0610 | 55 |
DQB1*0611 | 55 |
DQB1*0612 | 56 |
DQB1*0614 | 55 |
DQB1*0201 | 57, 58 |
DQB1*0202 | 57, 58 |
DQB1*0203 | 57, 58 |
DQB1*03011 | 57 |
DQB1*03012 | 57 |
DQB1*0302 | 57 |
DQB1*03032 | 57 |
DQB1*0304 | 57 |
DQB1*0305 | 57 |
DQB1*0306 | 57 |
DQB1*0307 | 57 |
DQB1*0308 | 57 |
DQB1*0401 | 57 |
Correspondence of DQB types and high accuracy typing capture oligos | |
Type | Corresponding capture oligos |
DQB1*0501 | 62,65,68,70,74,79,82,86,89,92,97,99,103,114,116,118,126,131,137,138 |
DQB1*0502 | 62,65,68,70,74,79,82,86,89,93,97,99,104,114,116,118,126,130,137,138 |
DQB1*05031 | 62,65,68,70,74,79,82,86,94,97,99,106,114,116,118,126,130,137,138 |
DQB1*05032 | 74,79,82,86,94,97,99,105,114,116 |
DQB1*0504 | 65,68,70,74,80,83,86,89,93,97,99,104,114,117,119,126,131 |
DQB1*06011 | 63,66,69,75,80,84,94,97,99,106,114,117,120,124,127,132,135,139 |
DQB1*06012 | 66,68,70,75,80,84,94,98,99,106,114,117,120,124,127,132,135,139 |
DQB1*06013 | 63,66,69,75,80,84,94,97,99,106,114,117,120,124,127,132,135,139 |
DQB1*0602 | 64,67,68,70,76,80,87,89,95,97,99,105,114,116,121,125,127,132,135,139 |
DQB1*0603 | 62,67,68,70,77,79,87,89,95,97,99,105,114,116,121,125,127,132,135,139 |
DQB1*0604 | 62,67,68,70,77,79,87,89,93,97,99,103,114,116,122,125,127,132,134,138 |
DQB1*06051 | 62,65,68,70,77,8087,89,93,97,99,103,114,116,122,125,127,132,134,138 |
DQB1*06052 | 77,80,87,89,93,97,99107,114,116,122,125,127,132 |
DQB1*0606 | 77,80,87,89,93,97,99,103,114,116,122,128,129,131 |
DQB1*0607 | 67,68,70,77,79,87,89,95,97,99,105,114,116,122,125,127,132 |
DQB1*0608 | 67,68,70,77,79,87,89,95,97,99,103,114,116,121,127,132 |
DQB1*0609 | 62,67,68,7077,80,87,89,93,97,99,103,114,116,122,125,127,132,134,138 |
DQB1*0610 | 64,67,68,70,76,80,87,89,95,97,99,104,114,116,121,125,127,132,135,139 |
DQB1*0611 | 67,68,70,76,80,87,89,95,97,99, 105,114,116,121,125,127,132,135,139 |
DQB1*0612 | 62,67,68,70,77,80,87,89,93,97,99,103,114,116,121,125,127,132,134,138 |
DQB1*0613 | 64,67,68,70,76,80,87,89,95,97,99,103,114,116,121,125,127,132,135 |
DQB1*0614 | 67,68,70,77,79,87,89,95,97,99, 105,114,116,121,125,127,132,135,139 |
DQB1*0201 | 60,61,62,67,71,78,81,85,90,100,108,114,116,123,128,129,131,136,140 |
DQB1*0202 | 59,60,61,62,67,71,78,81,85,90,100,108,114,117,123,128,129,131,136,140 |
DQB1*0203 | 62,67,71,78,81,85,90,100,109,114,116,117,123,128,129,131,136,140 |
DQB1*03011 | 62,66,68,70,75,80,88,91,93,101,110,114,116,122,125,127,132,136,140 |
DQB1*03012 | 66,68,70,75,80,87,91,93,101,110,114,116,122,125,127,132,136,140 |
DQB1*0302 | 62,67,68,70,76,80,88,94,101,111,114,116,122,125,127,132,136,140 |
DQB1*03032 | 62,67,68,70,76,80,88,94,101,110,114,116,122,125,127,132,136,140 |
DQB1*0304 | 62,66,68,70,75,80,88,91,93,101,111,114,116,122,125,127,132,136,140 |
DQB1*0305 | 62,67,72,74,80,87,94,101,111,114,116,122,125,127,132,136,140 |
DQB1*0306 | 62,67,68,70,76,80,88,94,101,110,115,117,119,126,133,136,140 |
DQB1*0307 | 62,67,68,70,76,80,88,96,101,111,114,116,122,125,127,132,136,140 |
DQB1*0308 | 62,67,68,70,76,80,88,94,101,111,114,116,121,125,127,132,136,140 |
DQB1*0401 | 64,67,73,74,80,87,94,102,112,113,115,117,119,126,133,136,140 |
DQB1*0402 | 64,67,72,74,80,87,94,102,112,113,115,117,119,126,133,136,140 |
Correspondence of DRA types and high accuracy typing capture oligos | |
Type | Corresponding capture oligos |
DRA*0101 | 141,143 |
DRA*0102 | 142,144 |
Correspondence of DRB1 types and low accuracy and high accuracy typing capture oligos (1), alleles whose serological antigen type is DR1 | ||
Type | Corresponding capture oligos | |
low accuracy | High accuracy | |
DRB1*0101 | 145 | 166, 173, 187, 213, 246, 250 |
DRB1*01021 | 145 | 166, 173, 187, 213, 232, 236, 250 |
DRB1*01022 | 145 | 166, 173, 187, 213, 228 ,232, 236, 250 |
DRB1*0103 | 145 | 166, 173, 187, 211, 215, 246, 250 |
DRB1*0104 | 145 | 173, 187, 213, 230, 250 |
DRB1*0105 | 145 | 173, 187, 192, 213, 246, 250 |
DRB1*0106 | 145 |
Correspondence of DRB1 types and low accuracy and high accuracy typing capture oligos (2), alleles whose serological antigen type is DR2 | ||
Type | Corresponding capture oligos | |
low accuracy | high accuracy | |
DRB1*15011 | 149 | 157,187,194,201,211 ,212, 234, 247 |
DRB1*15012 | 149 | 157,187,194,201,211,212,233,247 |
DRB1*15021 | 149 | 157,18,7194,201,211 ,212, 246, 247 |
DRB1*15022 | 187, 194, 211 ,212, 247, 250 | |
DRB1*15023 | 149 | 157,181,187,194,201,211, 212 |
DRB1*1503 | 149 | 157,178,187,194,201,211, 212, 234, 247 |
DRB1*1504 | 149 | 157, 187, 194, 201, 210, 212, 234, 247 |
DRB1*1505 | 149 | 157, 187, 194, 201, 212, 234, 247 |
DRB1*1506 | 149 | 187, 194, 196 201, 211 ,212, 247 |
DRB1*1507 | 149 | 157, 187, 201, 211, 212, 247 |
DRB1*1508 | 149 | 157, 187, 194 201 212, 246, 247 |
DRB1*1509 | 149 | |
DRB1*1510 | 149 | |
DRB1*16011 | 149 | 157, 187 201, 210 220, 246, 247 |
DRB1*16012 | 149 | 157, 187, 20, 210, 246, 247 |
DRB1*16021 | 149 | 157, 187, 201, 220, 246, 247 |
DRB1*16022 | 149 | 187, 201, 247 |
DRB1*1603 | 149 | 157, 187 201, 210 ,221, 246, 247 |
DRB1*1604 | 149 | 187, 201, 210, 222, 247 |
DRB1*1605 | 149 | 187, 201, 211, 220, 247 |
DRB1*1607 | 149 | 157, 187, 201, 211, 220, 241, 246, 247 |
DRB1*1608 | 149 | 157, 184 201, 210, 220, 246, 247 |
Correspondence of DRB1 Types and low accuracy and high accuracy typing capture oligos (3), alleles whose serological antigen type is DR3 | ||
Type | Corresponding capture oligos | |
low accuracy | high accuracy | |
DRB1*03011 | 146 | 171, 179, 184, 194, 216, 226, 229, 234, 229, 234, 248, 250 |
DRB1*03012 | 146 | 171, 179, 184, 194, 216, 226 ,230, 234 ,230, 234, 248, 250 |
DRB1*03021 | 146 | 174, 184 ,216 226, 229, 246, 248, 250 |
DRB1*03022 | 146 | 174, 184, 216, 226, 230, 246, 248, 250 |
DRB1*0303 | 146 | 174, 184, 216, 226, 229, 234, 248, 250 |
DRB1*0304 | 146 | 171, 179 ,187 ,194 ,216, 226 ,229, 248, 250 |
DRB1*0305 | 146 | 171, 179, 184, 194, 216, 226, 229, 246, 248, 250 |
DRB1*0306 | 146 | 171, 179, 184, 216, 229, 234,248, 250 |
DRB1*0307 | 146 | 179, 184, 194, 216, 226, 229, 234, 248, 250 |
DRB1*0308 | 146 | 171, 179, 184, 194, 206 ,216, 226, 229, 234, 248 |
DRB1*0309 | 146 | 171, 182, 184, 194, 216, 226. 229, 246, 250 |
DRB1*0310 | 146 | 171, 179, 184, 194, 200, 216, 226, 229, 234, 244, 248 |
DRB1*0311 | 146 | 171, 179, 184, 194, 216, 227, 229, 234, 248, 250 |
DRB1*0312 | 146 | 171, 179, 184, 194, 199, 216, 226, 229, 248 |
DRB1*0313 | 146 | |
DRB1*0314 | 146 | |
DRB1*0315 | 146 | |
DRB1*0316 | 146 | |
DRB1*0317 | 146 |
Correspondence of DRB1 types and low accuracy and high accuracy typing capture oligos (4), alleles whose serological antigen type is DR4 | ||
Type | Corresponding capture oligos | |
low accuracy | high accuracy | |
DRB1*04011 | 147 | 160, 169, 180, 216, 246, 249, 250 |
DRB1*04012 | 147 | 160, 169, 183, 216, 246, 249, 250 |
DRB1*0402 | 147 | 160, 169, 180, 211, 215, 234, 249, 250 |
DRB1*0403 | 160, 169, 180, 213, 223, 234, 249, 250 | |
DRB1*04031 | 147 | |
DRB1*04032 | 147 | 161, 169, 180, 201, 213, 223, 234, 249 |
DRB1*0404 | 147 | 160, 169, 180, 213, 234, 249, 250 |
DRB1*04051 | 147 | 160, 169, 180, 199, 213, 246, 249 |
DRB1*04052 | 147 | 160, 169, 180, 193, 199, 213, 246 |
DRB1*0406 | 147 | 160, 169, 180, 187, 213, 223, 234, 250 |
DRB1*0407 | 147 | 160, 169, 180, 213, 223, 246, 249, 250 |
DRB1*0408 | 147 | 160, 169, 180, 213, 246, 249, 250 |
DRB1*0409 | 169, 180, 199, 216, 249 | |
DRB1*0410 | 147 | 160, 169, 180, 199, 213, 234, 249 |
DRB1*0411 | 147 | 160, 169, 180, 199, 213, 223, 234, 249 |
DRB1*0412 | 147 | 160, 169, 199, 211, 222, 234, 249 |
DRB1*0413 | 169, 180, 216, 234, 249, 250 | |
DRB1*0414 | 169, 180, 211, 215, 249, 250 | |
DRB1*0415 | 147 | 160, 169, 180, 206, 210, 249 |
DRB1*0416 | 169, 180, 207, 216, 249 | |
DRB1*0417 | 169, 180, 199, 213, 223, 249 | |
DRB1*0418 | 180, 211, 222, 234, 249, 250 | |
DRB1*0419 | 169, 180, 187, 213, 246, 250 | |
DRB1*0420 | 169, 180, 187, 213, 223, 250 | |
DRB1*0421 | 147 | 160, 169, 180, 187, 216, 246, 250 |
DRB1*0422 | 147 | 160, 169, 180, 216, 226, 229, 234, 249, 250 |
DRB1*0423 | 147 | 160, 169, 180, 213, 245, 249, 250 |
DRB1*0424 | 147 | 160, 169, 180, 199, 214, 246, 249 |
DRB1*0425 | 147 | 160, 169, 180, 210, 222, 249, 250 |
DRB1*0426 | 147 | 160, 169, 180, 209, 216, 249 |
DRB1*0427 | 147 | 160, 169, 118, 213, 223, 236, 249, 250 |
DRB1*0428 | 147 | 160, 169, 180, 194, 199 ,213, 246, 249 |
DRB1*0429 | 147 | 160, 169, 180, 197, 199 ,213, 246, 249 |
DRB1*0430 | 147 | 160, 169, 180, 195, 213, 246, 249 |
DRB1*0431 | 147 | 160, 169, 180, 213, 222, 246, 249, 250 |
DRB1*0432 | 147 | 160, 169, 180, 219, 249,250 |
DRB1*0433 | 147 | |
DRB1*0434 | 147 |
Correspondence of DRB1 types and low accuracy and high accuracy typing capture oligos (5), alleles whose serological antigen type is DR5 and whose subtype is DR11 | ||
Type | Corresponding capture oligos | |
low accuracy | high accuracy | |
DRB1*11011 | 146 | 194, 206, 210, 246, 249 |
DRB1*11012 | 146 | 194, 206, 210, 239, 246, 249 |
DRB1*11013 | 146 | 194, 206, 210, 220, 246, 249 |
DRB1*1102 | 146 | 194, 206, 211, 215, 234, 249 |
DRB1*1103 | 146 | 194, 206, 210, 220, 246, 249 |
DRB1*11041 | 146 | 194, 206, 210, 234, 249 |
DRB1*11042 | 146 | 194, 206, 210, 234, 239, 249 |
DRB1*1105 | 146 | 194, 206, 210, 246, 249 |
DRB1*1106 | 146 | 194, 206, 210, 236, 249 |
DRB1*1107 | 146 | 194, 206, 216, 226, 229, 234, 249 |
DRB1*11081 | 194, 206, 246, 249 | |
DRB1*11082 | 194, 206, 246, 249 | |
DRB1*1109 | 179, 184, 194, 206, 210, 246, 248 | |
DRB1*1110 | 179, 188, 194, 206, 210, 248 | |
DRB1*1111 | 194, 206, 210, 215, 246, 249 | |
DRB1*1112 | 188, 194, 206, 210 | |
DRB1*1113 | 146 | 179, 188, 194, 206, 214, 232, 234, 248 |
DRB1*1114 | 146 | 194, 206, 211, 215, 246, 249 |
DRB1*1115 | 146 | 186, 194, 206, 210, 246 |
DRB1*1116 | 146 | 179, 184, 194, 206, 211, 215, 248 |
DRB1*1117 | 146 | 179, 188, 206, 214, 223, 232, 234, 248 |
DRB1*1118 | 146 | 194, 206, 211, 234, 249 |
DRB1*1119 | 146 | 194, 206, 211, 246, 249 |
DRB1*1120 | 146 | 179, 184, 194, 206, 211, 215, 246, 248 |
DRB1*1121 | 146 | 194, 206, 211, 215, 249 |
DRB1*1122 | 147 | 162, 194, 206 210, 246, 247, 249 |
DRB1*1123 | 146 | 194, 206, 210, 222, 249 |
DRB1*1124 | 146 | 185, 194, 206, 210 |
DRB1*1125 | 146 | 194, 206, 210, 222, 234, 249 |
DRB1*1126 | 146 | 194, 206, 213, 234, 249 |
DRB1*1127 | 194, 206, 210, 230, 249 | |
DRB1*11271 | 146 | |
DRB1*11272 | 146 | |
DRB1*1128 | 146 | 184, 194 206 |
DRB1*1129 | 146 | 187, 194, 206, 210, 246 |
DRB1*1130 | 154 | 165, 194, 206, 210, 246, 249 |
DRB1*1131 | 146 | 194, 208, 211, 246, 249 |
DRB1*1132 | 146 | 194, 206, 210, 224, 246, 249 |
DRB1*1133 | 146 | 194, 210, 242, 246, 249, 250 |
DRB1*1134 | 146 | 194, 206, 213, 234, 249 |
DRB1*1135 | 146 | 194, 210, 234, 242, 249, 250 |
DRB1*1136 | 146 | |
DRB1*1137 | 146 |
Correspondence of DRB1 types and low accuracy and high accuracy typing capture oligos (6), alleles whose serological antigen type is DR5 and whose subtype is DR12 | ||
Type | Corresponding capture oligos | |
low accuracy | high accuracy | |
DRB1*1201 | 146 | 158, 172, 191, 194, 202, 211, 220, 232, 236, 248 |
DRB1*12021 | 146 | 158, 172, 191, 194, 202, 210, 220, 232, 246, 248 |
DRB1*12022 | 146 | 158, 172, 191, 194, 202, 210, 220, 248 |
DRB1*12032 | 146 | 158, 172, 191, 194, 202, 211, 220, 234, 248 |
DRB1*1204 | 146 | 158, 172, 191, 194, 206, 211, 220, 232, 248 |
DRB1*1205 | 146 | 158, 172, 190, 194, 202, 211, 220, 232, 236, 248 |
DRB1*1206 | 146 |
Correspondence of DRB1 types and low accuracy and high accuracy typing capture oligos (7), alleles whose serological antigen type is DR6 and whose subtype is DR13 | ||
Type | Corresponding capture oligos | |
low accuracy | high accuracy | |
DRB1*1301 | 146 | 179, 184, 194, 211, 215, 234, 248, 250 |
DRB1*1302 | 179, 184, 194 211, 215, 246, 248, 250 | |
DRB1*13021 | 146 | |
DRB1*13022 | 146 | |
DRB1*12031 | 146 | 199, 211 218, 239, 246, 249 |
DRB1*13032 | 199, 211 218, 239, 246, 249 | |
DRB1*13032 | 146 | |
DRB1*1304 | 146 | 194, 199, 211, 215, 234, 249 |
DRB1*1305 | 146 | 179, 184, 194, 210, 246, 248, 250 |
DRB1*1306 | 146 | 179, 184, 194, 211, 234, 248, 250 |
DRB1*13071 | 146 | 210, 246, 249, 250 |
DRB1*13072 | 146 | 201, 210, 246, 249 |
DRB1*1308 | 146 | 179, 188, 211, 215, 234, 248, 250 |
DRB1*1309 | 146 | 179,184,194,211, 212, 234, 248, 250 |
DRB1*1310 | 146 | 179, 184, 194, 211, 218, 234, 248,250 |
DRB1*1311 | 146 | 194, 210, 249, 250 |
DRB1*1312 | 146 | 199, 211, 246, 249 |
DRB1*1313 | 146 | 199, 211, 222, 246, 249 |
DRB1*1314 | 194, 210, 246, 249, 250 | |
DRB1*13142 | 146 | |
DRB1*1315 | 146 | 174, 184, 194, 211, 215, 234, 248, 250 |
DRB1*1316 | 146 | 179, 184, 194, 211, 215, 235, 248, 250 |
DRB1*1317 | 146 | 158, 194, 211, 215, 234, 249, 250 |
DRB1*1318 | 146 | 179, 184, 194, 210, 222, 234, 248, 250 |
DRB1*1319 | 146 | 174, 188, 211, 215, 222, 234, 248, 250 |
DRB1*1320 | 146 | 179, 184, 194, 215, 234, 248, 250 |
DRB1*1321 | 146 | 194, 199, 210, 246, 249 |
DRB1*1322 | 146 | 194, 211, 215, 234, 249, 250 |
DRB1*1323 | 146 | 194, 211, 215, 239, 246, 249, 250 |
DRB1*1324 | 146 | 194, 210, 215, 234, 249, 250 |
DRB1*1325 | 146 | 194, 249, 250 |
DRB1*1326 | 146 | 174, 184, 201, 210, 220, 248 |
DRB1*1327 | 146 | 171, 179, 184, 194, 211, 215, 220, 234, 248, 250 |
DRB1*1328 | 146 | 179, 184, 194, 211 215, 237, 248, 250 |
DRB1*1329 | 146 | 179, 184, 194, 215, 246, 248, 250 |
DRB1*1330 | 146 | 194, 199 211, 246, 249 |
DRB1*1331 | 146 | 179, 184 194 211 215, 246, 248, 250 |
DRB1*1332 | 146 | 179, 184, 199, 211, 215, 234, 248 |
DRB1*1333 | 146 | 199, 211, 218, 225, 229, 249 |
DRB1*1334 | 146 | 179, 189, 194 ,211, 215, 240, 246, 248, 250 |
DRB1*1335 | 146 | |
DRB1*1336 | 146 | |
DRB1*1337 | 146 | |
DRB1*1338 | 146 | |
DRB1*1339 | 146 | |
DRB1*1340 | 146 | |
DRB1*1341 | 146 |
Correspondence of DRB1 types and low accuracy and high accuracy typing capture oligos (8), alleles whose serological antigen type is DR6 and whose subtype is DR14 | ||
Type | Corresponding capture oligos | |
low accuracy | high accuracy | |
DRB1*1401 | 146 | 179, 188, 200, 214, 223, 232, 234, 244, 248 |
DRB1*1402 | 146 | 174, 184, 213, 246, 248, 250 |
DRB1*1403 | 146 | 174, 184, 222, 246, 248, 250 |
DRB1*1404 | 146 | 158, 179, 188, 200, 214, 223, 232, 234, 244, 248 |
DRB1*1405 | 146 | 159, 179, 188, 205, 214, 223, 232, 234, 248 |
DRB1*1406 | 146 | 174, 184, 213, 234, 248, 250 |
DRB1*1407 | 146 | 179, 188, 200, 214, 223, 232, 244, 248 |
DRB1*1408 | 146 | 179, 188, 203, 214, 223, 232, 234, 244, 248 |
DRB1*1409 | 146 | 179, 184, 213, 246, 248, 250 |
DRB1*1410 | 147 | 160, 179, 188, 200, 214, 223, 232, 234, 244, 248 |
DRB1*1411 | 146 | 158, 179, 188, 206, 214, 223, 232, 248 |
DRB1*1412 | 174, 184, 222, 248, 250 | |
DRB1*1413 | 146 | 174, 184, 199, 213, 248 |
DRB1*1414 | 146 | 179, 188, 214, 223, 232, 246, 248, 250 |
DRB1*1415 | 158, 179, 188, 210, 222, 234, 248, 250 | |
DRB1*1416 | 146 | 179, 188, 200, 211, 215, 234, 244, 248 |
DRB1*1417 | 146 | 179, 184, 194, 213, 234, 248, 250 |
DRB1*1418 | 146 | 174, 184, 205, 214, 223, 232, 234, 248 |
DRB1*1419 | 146 | 174, 184, 216, 246, 248, 250 |
DRB1*1420 | 146 | 174, 188, 213, 248, 250 |
DRB1*1421 | 146 | 179, 184, 194, 216, 248, 250 |
DRB1*1422 | 146 | 179, 188 200, 210, 244, 246, 248 |
DRB1*1423 | 146 | 179, 188, 214, 223, 232, 234, 248, 250 |
DRB1*1424 | 146 | 174, 184, 211 212, 246, 248, 250 |
DRB1*1425 | 146 | 200, 210, 244, 249 |
DRB1*1426 | 146 | 170, 179, 188, 200, 214, 223, 232, 234, 244, 248 |
DRB1*1427 | 146 | 174, 184 210, 222, 246, 248, 250 |
DRB1*1428 | 146 | 158, 179,188, 200, 214, 223, 232, 244, 248 |
DRB1*1429 | 146 | 174, 184, 213, 236, 248, 250 |
DRB1*1430 | 146 | 179, 184 194, 213, 246, 248, 250 |
DRB1*1431 | 146 | 158, 179, 188, 200, 214, 232, 234, 244, 2480 |
DRB1*1432 | 146 | |
DRB1*1433 | 146 | 179, 184, 194, 213, 223, 234, 248, 250 |
DRB1*1434 | 146 | |
DRB1*1435 | 146 | |
DRB1*1436 | 146 |
Correspondence of DRB1 types and low accuracy and high accuracy typing capture oligos (9), alleles whose serological antigen type is DR7, DR8, DR9 or DR10 | ||
Type | Corresponding capture oligos | |
low accuracy | high accuracy | |
DRB1*0701 | 164, 169, 170, 176, 188, 198, 202, 211, 217, 227, 231, 246, 247 | |
DRB1*07011 | 148 | |
DRB1*07012 | 148 | |
DRB1*0703 | 148 | 164, 169, 170, 177, 188, 198, 202, 211, 217, 227, 231, 247 |
DRB1*0704 | 148 | 164, 169, 170, 176, 188, 198, 202, 211, 217, 227, 230, 247 |
DRB1*0801 | 146 | 158, 199, 210, 222, 239, 246, 249 |
DRB1*08021 | 146 | 158, 210, 222, 246, 249, 250 |
DRB1*08022 | 146 | 158, 210, 222, 246, 249, 250 |
DRB1*08032 | 146 | 158, 199, 211, 222, 246, 249 |
DRB1*08041 | 146 | 158, 210, 222, 234, 249, 250 |
DRB1*08042 | 158, 210, 222, 238, 239, 249, 250 | |
DRB1*08043 | 146 | 158, 210, 222, 238, 239, 249, 250 |
DRB1*0805 | 146 | 158, 199, 210, 249 |
DRB1*0806 | 146 | 158, 199, 210, 222, 234, 249 |
DRB1*0807 | 146 | 158, 210, 222, 239, 246, 249, 250 |
DRB1*0808 | 146 | 158, 200, 210, 222, 244, 249 |
DRB1*0809 | 168, 179, 188, 210, 222, 239, 246, 248, 250 | |
DRB1*0810 | 146 | 158, 199, 211, 222, 234, 249 |
DRB1*0811 | 146 | 158, 210, 222, 239, 246, 249, 250 |
DRB1*0812 | 146 | 158, 199, 211, 222, 249 |
DRB1*0813 | 146 | 158, 222, 249, 250 |
DRB1*0814 | 146 | 167, 199, 211 222, 2467, 249 |
DRB1*0815 | 146 | 158203211222, 244, 246, 249 |
DRB1*0816 | 146 | 158,185,199,210,222 |
DRB1*0817 | 146 | 158, 194, 199, 210, 222, 249 |
DRB1*0818 | 146 | 158, 199, 211, 246, 249 |
DRB1*0819 | 146 | 58, 211 222, 246, 249, 250 |
DRB1*0820 | 146 | |
DRB1*0821 | 146 | 168, 179, 188, 210, 222, 239, 246, 248, 250 |
DRB1*09012 | 150 | 163, 169, 175, 184, 202, 210, 214, 223, 231, 243 |
DRB1*10011 | 151 | |
DRB1*10012 | 151 |
Correspondence of DRB3 types and low accuracy typing and high accuracy typing capture oligos | ||
Type | Corresponding capture oligos | |
low accuracy | High accuracy | |
DRB3*01011 | 152 | 252, 255, 258, 260, 265, 267, 271, 273, 275, 277, 279 |
DRB3*01012 | 152 | 252, 255, 258, 260, 265, 267, 271, 273, 275, 277, 279 |
DRB3*01013 | 152 | 252, 255, 258, 260, 265, 268, 271, 273, 275, 277, 279 |
DRB3*01014 | 152 | 252, 255, 258, 260, 265, 267, 271, 273, 275, 278, 279 |
DRB3*0102 | 153 | 253, 255, 258, 260, 265, 267, 271, 273, 275, 277, 279 |
DRB3*0103 | 152 | 252, 255, 258, 260, 265, 267, 271, 273, 275, 277, 279 |
DRB3*0104 | 152 | 252, 255, 258, 260, 265, 267, 271, 273, 275, 277, 279 |
DRB3*0105 | 152 | 252, 256, 258, 260, 265, 267, 271, 273, 275, 277, 279 |
DRB3*0106 | 152 | 252, 255, 258, 261, 265, 267, 271, 273, 275, 277, 279 |
DRB3*0107 | 152 | 252, 255, 258, 262, 266, 269, 272, 273, 275, 277, 279 |
DRB3*0201 | 154 | 254, 257, 259, 262, 266, 269, 272, 273, 275, 277, 280 |
DRB3*02021 | 154 | 254, 257, 259, 262, 266, 269, 272, 273, 275, 277, 279 |
DRB3*02022 | 154 | 254, 257, 259, 262, 266, 269, 272, 274, 275, 277, 279 |
DRB3*02023 | 154 | 254, 257, 259, 262, 266, 269, 272, 273, 276, 277, 279 |
DRB3*0203 | 154 | 254, 257, 259, 263, 266, 269, 272, 273, 275, 277, 279 |
DRB3*0204 | 154 | 254, 257, 259, 262, 266, 269, 271, 274, 275, 277, 280 |
DRB3*0205 | 154 | 254, 257, 258, 262, 266, 269, 272, 273, 275, 277, 279 |
DRB3*0206 | 154 | 254, 257, 259, 264, 266, 269, 272, 273, 275, 277, 279 |
DRB3*0207 | 154 | 254, 257, 259, 262, 266, 267, 272, 273, 275, 277, 279 |
DRB3*0208 | 154 | 254, 257, 259, 262, 266, 270, 272, 273, 275, 277, 279 |
DRB3*0210 | 154 | 254, 257, 259, 262, 265, 269, 272, 273, 275, 277, 279 |
DRB3*0211 | 154 | 254, 257, 259, 262, 266, 269, 272, 273, 275, 277, 279 |
DRB3*0212 | 154 | 254, 257, 259, 262, 266, 269, 272, 273, 275, 277, 279 |
DRB3*0301 | 154 | 254, 257, 258, 261, 265, 267, 272, 273, 275, 277, 280 |
DRB3*0302 | 154 | 254, 257, 259, 261, 265, 267, 272, 273, 275, 277, 280 |
DRB3*0303 | 154 | 254, 257, 258, 261, 265, 267, 271, 273, 275, 277, 279 |
Correspondence of DRB4 types and low accuracy typing and high accuracy typing capture oligos | ||
Type | Corresponding capture oligos | |
low accuracy | high accuracy | |
DRB4*01011 | 155 | 281, 284, 286, 288 |
DRB4*0102 | 282, 284, 287 | |
DRB4*0103101 | 155 | 281, 284, 287, 288 |
DRB4*0103102 N | 287 | |
DRB4*01032 | 155 | 281, 284, 287, 289 |
DRB4*0104 | 155 | 283, 284 |
DRB4*0105 | 155 | 281, 285 |
DRB4*0201N | 155 | 281, 284, 286 |
DRB4*0301N | 286 |
Correspondence of DRB5 types and low accuracy typing and high accuracy typing capture oligos | ||
Type | Corresponding capture oligos | |
low accuracy | high accuracy | |
DRB5*01011 | 156 | 290, 292, 295, 297, 302, 304, 306 |
DRB5*01012 | 156 | 290, 292, 295, 297, 300, 305, 306 |
DRB5*0102 | 156 | 291, 293, 295, 297, 302, 304, 306 |
DRB5*0103 | 156 | 291, 293, 295, 298, 304, 306 |
DRB5*0104 | 156 | 290, 292, 295, 297, 303, 306 |
DRB5*0105 | 156 | 290, 294, 295, 297, 302, 304, 306 |
DRB5*0106 | 156 | 290, 292, 296, 299, 301, 305, 307 |
DRB5*0107 | 156 | 290, 292, 296, 297, 302, 305, 307 |
DRB5*0108 N | 156 | 291, 293, 295, 297, 302, 304, 306 |
DRB5*0109 | 156 | 290, 292, 295, 297, 302, 304, 306 |
DRB5*0110 N | 156 | 291, 293, 295, 297, 302, 304, 306 |
DRB5*0202 | 156 | 291, 293, 296, 299, 301, 305, 306 |
DRB5*0203 | 156 | 291, 293, 296, 299, 301, 305, 306 |
DRB5*0204 | 156 | 291, 293, 295, 299, 301, 305, 307 |
Correspondence of DRB2, DRB6 and DRB7 types and low accuracy typing and high accuracy typing capture oligos | ||
Type | Corresponding capture oligos | |
low accuracy | high accuracy | |
DRB2*0101 | 251 | |
DRB6*0101 | 155 | 308, 310, 312, 314, 316, 318 |
DRB6*0201 | 155 | 309, 311, 313, 315, 317, 319 |
DRB6*0202 | 309, 311, 313, 315, 317, 320 | |
DRB7*01011 | 321 | |
DRB7*01012 | 322 | |
DRB7*01011 | 155 | 323 |
Correspondence of DPA types and high accuracy typing capture oligos | |
Type | Corresponding capture oligos |
DPA1*0103 | 324, 326, 330, 331, 333, 336, 338, 341, 343, 345, 347 |
DPA1*0104 | 324, 326, 330, 331, 334, 336, 338, 341, 343, 345, 347 |
DPA1*0105 | 324, 326, 330, 331, 333, 336, 338, 341, 343, 345, 348 |
DPA1*0106 | 324, 326, 330, 331, 333, 337, 339, 341, 343, 345, 347 |
DPA1*02011 | 324, 327, 330, 331, 333, 337, 339, 342, 343, 345, 348 |
DPA1*02012 | 324, 326, 330, 331, 333, 337, 339, 342, 343, 345, 348 |
DPA1*02013 | 324, 326, 330, 331, 333, 337, 338, 342, 343, 345 |
DPA1*02014 | 324, 330, 331, 333, 337, 339, 342, 343, 345, 348 |
DPA1*02021 | 325, 327, 329, 331, 333, 337, 340, 342, 343, 345, 348 |
DPA1*02022 | 325, 327, 329, 331, 333, 337, 338, 342, 343, 345, 348 |
DPA1*0203 | 324, 327, 330, 331, 333, 336, 338, 342, 343, 345, 347 |
DPA1*0301 | 325, 327, 330, 331, 333, 336, 338, 341, 344, 345, 347 |
DPA1*0302 | 325, 327, 330, 331, 333, 336, 338, 341, 343, 345, 347 |
DPA1*0401 | 324, 326, 328, 332, 335, 336, 338, 342, 343, 346, 348 |
Correspondence of DPB high accuracy typing capture (1) | |
Type | Corresponding capture oligos |
DPB1*01011 | 349, 254, 358, 361, 366, 367, 376 |
DPB1*01012 | 350, 355, 358, 364, 365, 368, 373, 376 |
DPB1*02011 | 350, 354, 358, 364, 365, 368, 373, 376 |
DPB1*02012 | 350, 354, 358, 364, 365, 369, 376 |
DPB1*02013 | 350, 354, 358, 362, 365, 371, 376 |
DPB1*0202 | 349, 356, 358, 364, 365, 370, 374, 377 |
DPB1*0301 | 350, 354, 358, 363, 365, 367, 376 |
DPB1*0401 | 350, 354, 358, 364, 365, 368, 373, 376 |
DPB1*0402 | 350, 354, 358, 362, 365, 371, 376 |
DPB1*0501 | 349, 356, 358, 364, 365, 370, 374, 377 |
DPB1*0601 | 350, 354, 358, 364, 365, 368, 373, 376 |
DPB1*0801 | 351, 356, 358, 364, 365, 370, 374, 376 |
DPB1*0901 | 351, 356, 358, 364, 365, 368, 373, 376, 386, 388, 390, 393, 396 |
DPB1*1001 | 349, 356, 360, 361, 366, 367, 377, 384, 385, 388, 391, 393, 396 |
DPB1*11011 | 349, 356, 359, 361, 366, 367, 377, 384, 386, 388, 391, 393, 396 |
DPB1*11012 | 349, 356, 358, 361, 365, 367, 376, 386, 388, 392, 393, 396 |
DPB1*1301 | 351, 356, 358, 364, 365, 370, 374, 377, 382, 386, 388, 390, 393, 396 |
DPB1*1401 | 349, 354, 360, 361, 366, 367, 377, 384, 386, 388, 391, 395, 396 |
DPB1*1501 | 350, 354, 358, 364, 365, 368, 373, 376, 386, 388, 391, 393, 396 |
DPB1*1601 | 351, 356, 358, 364, 365, 370, 374, 376, 386, 388, 391, 393, 396 |
DPB1*1701 | 349, 354, 358, 364, 365, 368, 373, 376, 382, 386, 388, 391, 395, 396 |
DPB1*1801 | 350, 354, 358, 364, 365, 371, 376, 386, 388, 392, 393, 396 |
DPB1*1901 | 349, 356, 358, 364, 365, 370, 374, 377, 382, 386, 388, 391, 393, 396 |
DPB1*20011 | 349, 356, 358, 364, 365, 370, 374, 377, 382, 386, 388, 391, 393, 396 |
DPB1*20012 | 349, 356, 358, 362, 365, 371, 376, 386, 388, 391, 393, 396 |
DPB1*2101 | 350, 354, 358, 362, 365, 371, 376, 386, 388, 391, 393, 396 |
DPB1*2201 | 350, 354, 358, 364, 365, 367, 376, 382, 386, 388, 391, 394, 396 |
DPB1*2301 | 350, 354, 358, 363, 365, 371, 376, 382, 386, 388, 391, 394, 396 |
DPB1*2401 | 349, 356, 358, 364, 365, 368, 373, 377, 382, 386, 388, 390, 393, 396 |
DPB1*2501 | 349, 356, 358, 361, 366, 367, 376, 382, 386, 388, 393, 396 |
DPB1*26011 | 349, 356, 358, 361, 365, 367, 376, 382, 386, 388, 390, 393, 396 |
DPB1*26012 | 349, 356, 358, 361, 365, 367, 376, 382, 386, 388, 391, 393, 396 |
DPB1*2701 | 350, 354, 368, 363, 365, 368, 373, 377, 382, 386, 388, 391, 395, 396 |
DPB1*2801 | 349, 356, 358, 364, 365, 370, 374, 377, 386, 388, 390, 393, 396 |
DPB1*2901 | 351, 356, 358, 364, 365, 371, 376, 386, 388, 391, 393, 396 |
DPB1*3001 | 350, 354, 358, 363, 365, 367, 377, 382, 387, 391, 393, 396 |
DPB1*3101 | 350, 354, 358, 364, 365, 372, 375, 376, 386, 388, 391, 394, 396 |
DPB1*3201 | 350, 354, 358, 363, 365, 367, 376, 386, 388, 391, 394, 396 |
DPB1*3301 | 350, 354, 358, 362, 365, 367, 377, 382, 387, 391, 395, 396 |
DPB1*3401 | 351, 356, 358, 364, 365, 370, 374, 376, 382, 386, 388, 390, 393, 396 |
DPB1*3501 | 349, 356, 358, 362, 365, 371, 376, 382, 386, 388, 391, 393, 396 |
DPB1*3601 | 349, 356, 358, 364, 365, 368, 373, 376, 386, 388, 390, 393, 396 |
DPB1*3701 | 350, 354, 358, 362, 365, 371, 376, 382, 386, 388, 391, 393, 397 |
DPB1*3801 | 350, 354, 358, 361, 365, 367, 376, 382, 386, 388, 391, 394, 396 |
DPB1*3901 | 350, 354, 358, 361, 365, 367, 376, 382, 386, 388, 391, 395, 396 |
DPB1*4001 | 350, 354, 358, 364, 365, 368, 373, 378, 386, 388, 391, 394, 396 |
Correspondence of DPB types and high accuracy typing capture oligos (2) | |
Type | Corresponding capture oligos |
DPB1*4101 | 349, 356, 358, 362, 365, 370, 374, 377, 386, 388, 390, 393, 396 |
DPB1*4401 | 351, 356, 358, 364, 365, 368, 373, 377, 382, 386, 388, 390, 393, 396 |
DPB1*4501 | 350, 354, 358, 364, 365, 370, 374, 376, 386, 388, 391, 394, 396 |
DPB1*4601 | 350, 354, 358, 364, 365, 371, 376, 386, 388, 391, 394, 396 |
DPB1*4701 | 350, 354, 358, 362, 365, 368, 373, 376, 386, 388, 391, 394, 396 |
DPB1*4801 | 350, 354, 358, 361, 365, 368, 373, 376, 382, 386, 388, 391, 394, 396 |
DPB1*4901 | 349, 354, 358, 364, 365, 370, 374, 377, 382, 386, 388, 390, 393, 396 |
DPB1*5001 | 350, 354, 358, 363, 365, 368, 373, 376, 382, 386, 388, 391, 394, 396 |
DPB1*5101 | 349, 356, 358, 364, 365, 367, 377, 382, 386, 388, 390, 393, 396 |
DPB1*5201 | 350, 354, 358, 361, 365, 368, 373, 376, 382, 386, 388, 391, 395, 396 |
DPB1*5301 | 351, 356, 358, 364, 365, 371, 376, 386, 388, 390, 393, 396 |
DPB1*5401 | 351, 356, 358, 364, 365, 367, 376, 386, 388, 391, 393, 396 |
DPB1*5501 | 349, 356, 358, 363, 365, 367, 377, 382, 386, 388, 390, 393, 396 |
DPB1*5601 | 350, 354, 358, 364, 365, 370, 374, 377, 382, 386, 388, 390, 393, 396 |
DPB1*5701 | 351, 356, 358, 362, 365, 367, 376, 386, 388, 391, 393, 396 |
DPB1*5801 | 350, 354, 358, 364, 365, 368, 373, 377, 382, 386, 388, 391, 394, 396 |
DPB1*5901 | 350, 354, 358, 364, 365, 368, 373, 379, 382, 386, 388, 391, 394, 396 |
DPB1*6001 | 349, 356, 358, 364, 365, 370, 374, 380, 381, 385, 386, 388, 390, 393 |
DPB1*6101N | 350, 354, 358, 362, 365, 367, 376, 382, 386, 388, 391, 395, 396 |
DPB1*6201 | 350, 354, 358, 362, 365, 367, 376, 382, 386, 388, 391, 393, 396 |
DPB1*6301 | 352, 356, 358, 364, 365, 370, 374, 377, 386, 388, 391, 393, 396 |
DPB1*6401N | 350, 354, 358, 361, 365, 367, 376, 382, 386, 388, 390, 393, 396 |
DPB1*6501 | 351, 356, 358, 363, 365, 367, 376, 382, 386, 388, 391, 394, 396 |
DPB1*6601 | 351, 356, 358, 364, 365, 367, 377, 382, 386, 388, 390, 393, 396 |
DPB1*6701 | 350, 354, 358, 364, 365, 368, 373, 376, 382, 386, 388, 390, 393, 396 |
DPB1*6801 | 349, 356, 358, 364, 365, 370, 374, 377, 384, 386, 388, 391, 393, 396 |
DPB1*6901 | 353, 356, 358, 364, 365, 370, 374, 377, 382, 386, 388, 390, 393, 396 |
DPB1*7001 | 350, 354, 358, 364, 365, 367, 376, 386, 388, 391, 394, 396 |
DPB1*7101 | 350, 354, 358, 363, 365, 367, 377, 382, 386, 388, 391, 394, 396 |
DPB1*7201 | 350, 354, 358, 364, 365, 368, 373, 377, 382, 386, 388, 390, 394, 396 |
DPB1*7301 | 349, 356, 359, 364, 366, 367, 377, 386, 388, 391, 395, 396 |
DPB1*7401 | 350, 354, 358, 364, 365, 368, 373, 376, 382, 386, 388, 390, 394, 396 |
DPB1*7501 | 351, 356, 358, 363, 365, 368, 373, 377, 382, 386, 388, 390, 393, 396 |
DPB1*7601 | 350, 357, 358, 361, 365, 368, 373, 376, 382, 386, 388, 391, 394, 396 |
DPB1*7701 | 349, 356, 358, 364, 365, 370, 374, 377, 382, 389, 390, 393, 396 |
DPB1*7801 | 349, 356, 358, 364, 365, 368, 373, 376, 382, 386, 388, 390, 393, 396 |
DPB1*7901 | 349, 356, 358, 364, 365, 368, 373, 376, 382, 386, 388, 390, 393, 396 |
Brief Description of the Drawings
Fig. 1 shows positions of capture oligos (for HLA-DQA1) immobilized on a substrate. Numbers in the figure represent sequence numbers (the same shall apply in Figs. 2-7).
Fig. 2 shows positions of capture oligos (for HLA-DQB1) immobilized on a substrate.
Fig. 3 shows positions of capture oligos (for HLA-DRA) immobilized on a substrate.
Fig. 4 shows positions of capture oligos (for HLA-DRB1) immobilized on a substrate.
Fig. 5 shows positions of capture oligos (for HLA-DRB3, HLA-DRB4, HLA-DRB5 and HLA-DRB6) immobilized on a substrate.
Fig. 6 shows positions of capture oligos (for HLA-DPA1) immobilized on a substrate.
Fig. 7 shows positions of capture oligos (for HLA-DPB1) immobilized on a substrate.
Fig. 8 shows a schematic view of a kit of the present invention and a detection result of DRA typing using the same (detection result typed as DRA*0101).
Fig. 9 shows a schematic view of the kit of the present invention and a detection result of DRB low resolution typing using the same (detection result typed as DR4).
Fig. 10 shows a schematic view of the kit of the present invention and a detection result of DRB1 high resolution typing using the same (detection result typed as DRB*04011).
Fig. 11 shows positions of capture oligos (for HLA-DPB1) immobilized on a substrate.
Fig. 12 shows positions of capture oligos (for HLA-DQB1) immobilized on a substrate.
Fig. 13 shows positions of capture oligos (for HLA-DRB1) immobilized on a substrate.
Fig. 14 shows positions of capture oligos (for HLA-DRB pseudogene) immobilized on a substrate.
Fig. 15 shows positions of capture oligos (for HLA-DPB3, 4, 5) immobilized on a substrate.
Fig. 16 shows positions of capture oligos (for HLA-A) immobilized on a substrate.
Fig. 17 shows positions of capture oligos (for HLA-B) immobilized on a substrate.
Fig. 18 shows a schematic view of the kit of the present invention (A) and a photograph showing detection result of DPB1 typing using the same (detection result typed as DPB1*01011) (B).
Fig. 19 shows a schematic view of the kit of the present invention (A) and a photograph showing detection result of DQB1 typing using the same (detection result typed as DQB1*05031) (B).
Fig. 20 shows a schematic view of the kit of the present invention (A) and a photograph showing detection result of DRB1 typing using the same (detection result typed as DRB1*0101) (B).
Fig. 21 shows a schematic view of the kit of the present invention (A) and a photograph showing detection result of DRB9 typing using the same (detection result typed as DRB9 0101) (B).
Fig. 22 shows a schematic view of the kit of the present invention (A) and a photograph showing detection result of DRB3 typing using the same (detection result typed as DRB3 0301) (B).
Fig. 23 shows a schematic view of the kit of the present invention (A) and a photograph showing detection result of A typing using the same (detection result typed as A 01011) (B).
Fig. 24 shows a schematic view of the kit of the present invention (A) and a photograph showing detection result of B typing using the same (detection result typed as B 0724) (B).
Best Mode for Carrying out the Invention
Example 1. Synthesis of oligonucleotides
Example 2. Spotting of capture oligos on substrate (utilizing oligonucleotide having amino group at 5' end)
Example 3. Spotting of capture oligos on substrate (utilizing oligonucleotide having hydroxyl group at 5' end)
Example 4. Preparation of nucleic acid target
Example 5. Hybridization
Example 6. Detection of chemical color development
Example 7. Typing using Patchworks
Correspondence of the patchwork regions and satellite regions for types of DPB1 | |||||||||||
patchwork region | satellite region | ||||||||||
DPB1‡01011 | DPB1 | DPB4 | DPB7 | DPB12 | DPB15 | DPB18 | DPB21 | ||||
DPB1‡6501 | DPB1 | DPB4 | DPB7 | DPB12 | DPB15 | DPB18 | DPB21 | ||||
DPB1‡01012 | DPB1 | DPB4 | DPB7 | DPB12 | DPB15 | DPB18 | DPB21 | 473 | |||
DPB1‡8901 | DPB1 | DPB4 | DPB7 | DPB12 | DPB15 | DPB19 | DPB21 | ||||
DPB1‡1301 | DPB2 | DPB4 | DPB7 | DPB12 | DPB16 | DPB20 | DPB21 | ||||
DPB1‡26011 | DPB2 | DPB4 | DPB7 | DPB12 | DPB15 | DPB18 | DPB21 | 473 | 495 | ||
DPB1‡26012 | DPB2 | DPB4 | DPB7 | DPB12 | DPB15 | DPB18 | DPB21 | ||||
DPB1‡2701 | DPB2 | DPB4 | DPB7 | DPB12 | DPB15 | DPB19 | DPB21 | ||||
DPB1‡8501 | DPB2 | DPB4 | DPB7 | DPB12 | DPB15 | DPB19 | DPB21 | 456 | |||
DPB1‡3901 | DPB1 | DPB4 | DPB7 | DPB12 | DPB15 | DPB19 | DPB22 | ||||
DPB1‡4001 | DPB1 | DPB4 | DPB7 | DPB12 | DPB15 | DPB19 | DPB23 | ||||
DPB1‡0401 | DPB1 | DPB3 | DPB7 | DPB12 | DPB15 | DPB19 | DPB22 | ||||
DPB1‡3301 | DPB1 | DPB3 | DPB7 | DPB12 | DPB16 | DPB19 | DPB22 | ||||
DPB1‡6601 | DPB2 | DPB3 | DPB7 | DPB12 | DPB15 | DPB19 | DPB22 | 456 | |||
DPB1‡2301 | DPB1 | DPB5 | DPB7 | DPB12 | DPB15 | DPB19 | DPB22 | ||||
DPB1‡7101 | DPB1 | DPB5 | DPB7 | DPB12 | DPB16 | DPB19 | DPB22 | ||||
DPB1‡5501 | DPB2 | DPB5 | DPB7 | DPB12 | DPB16 | DPB19 | DPB21 | 456 | |||
DPB1‡5801 | DPB2 | DPB6 | DPB7 | DPB12 | DPB16 | DPB19 | DPB21 | 456 | |||
DPB1‡6301 | DPB1 | DPB6 | DPB7 | DPB12 | DPB15 | DPB19 | DPB21 | ||||
DPB1‡6201 | DPB1 | DPB6 | DPB7 | DPB12 | DPB15 | DPB19 | DPB23 | ||||
DPB1‡1601 | DPB1 | DPB4 | DPB8 | DPB12 | DPB16 | DPB19 | DPB21 | ||||
DPB1‡4901 | DPB1 | DPB4 | DPB8 | DPB12 | DPB15 | DPB19 | DPB22 | ||||
DPB1‡5301 | DPB1 | DPB4 | DPB8 | DPB12 | DPB15 | DPB19 | DPB23 | ||||
DPB1‡0801 | DPB1 | DPB5 | DPB8 | DPB12 | DPB16 | DPB18 | DPB21 | ||||
DPB1‡6801 | DPB1 | DPB5 | DPB8 | DPB12 | DPB15 | DPB18 | DPB21 | ||||
DPB1‡7501 | DPB1 | DPB5 | DPB8 | DPB12 | DPB15 | DPB18 | DPB22 | ||||
DPB1‡02012 | DPB1 | DPB5 | DPB8 | DPB12 | DPB16 | DPB19 | DPB22 | ||||
DPB1‡3201 | DPB1 | DPB5 | DPB8 | DPB12 | DPB16 | DPB19 | DPB22 | 481 | |||
DPB1‡4601 | DPB1 | DPB5 | DPB8 | DPB12 | DPB16 | DPB19 | DPB22 | 482 | |||
DPB1‡6001 | DPB1 | DPB5 | DPB8 | DPB12 | DPB15 | DPB19 | DPB22 | 487 | |||
DPB1‡7701 | DPB1 | DPB5 | DPB8 | DPB12 | DPB15 | DPB19 | DPB22 | 464 | |||
DPB1‡8001 | DPB1 | DPB5 | DPB8 | DPB12 | DPB15 | DPB19 | DPB22 | ||||
DPB1‡8201 | DPB1 | DPB5 | DPB8 | DPB12 | DPB15 | DPB19 | DPB22 | ||||
DPB1‡0901 | DPB2 | DPB5 | DPB8 | DPB12 | DPB16 | DPB18 | DPB21 | 482 | 456 | ||
DPB1‡1001 | DPB2 | DPB5 | DPB8 | DPB12 | DPB16 | DPB18 | DPB21 | 456 | |||
DPB1‡3701 | DPB2 | DPB5 | DPB8 | DPB12 | DPB16 | DPB18 | DPB21 | ||||
DPB1‡3501 | DPB2 | DPB5 | DPB8 | DPB12 | DPB15 | DPB18 | DPB21 | 482 | 456 | ||
DPB1‡7901 | DPB2 | DPB5 | DPB8 | DPB12 | DPB15 | DPB18 | DPB21 | ||||
DPB1‡8801 | DPB2 | DPB5 | DPB8 | DPB12 | DPB16 | DPB18 | DPB21 | 482 | |||
DPB1‡1701 | DPB2 | DPB5 | DPB8 | DPB12 | DPB16 | DPB19 | DPB21 | 482 | 456 | ||
DPB1‡0402 | DPB2 | DPB5 | DPB8 | DPB12 | DPB15 | DPB19 | DPB22 | ||||
DPB1‡1801 | DPB1 | DPB5 | DPB8 | DPB12 | DPB15 | DPB19 | DPB23 | ||||
DPB1‡4801 | DPB1 | DPB6 | DPB8 | DPB12 | DPB16 | DPB19 | DPB22 | ||||
DPB1‡5101 | DPB1 | DPB3 | DPB8 | DPB12 | DPB15 | DPB19 | DPB22 | ||||
DPB1‡8101 | DPB1 | DPB3 | DPB8 | DPB12 | DPB16 | DPB19 | DPB22 | ||||
DPB1‡02013 | DPB1 | DPB5 | DPB9 | DPB12 | DPB16 | DPB19 | DPB22 | ||||
DPB1‡4701 | DPB1 | DPB5 | DPB10 | DPB12 | DPB16 | DPB19 | DPB22 | ||||
DPB1‡1901 | DPB1 | DPB5 | DPB10 | DPB12 | DPB16 | DPB20 | DPB21 | ||||
DPB1‡3001 | DPB2 | DPB5 | DPB10 | DPB12 | DPB16 | DPB19 | DPB21 | 456 | |||
DPB1‡5401 | DPB2 | DPB5 | DPB10 | DPB12 | DPB16 | DPB18 | DPB21 | 456 | |||
DPB1‡0202 | DPB1 | DPB6 | DPB10 | DPB12 | DPB16 | DPB19 | DPB22 | ||||
DPB1‡2201 | DPB1 | DPB6 | DPB10 | DPB12 | DPB16 | DPB19 | DPB21 | ||||
DPB1‡0501 | DPB1 | DPB6 | DPB10 | DPB12 | DPB15 | DPB19 | DPB21 | ||||
DPB1‡3801 | DPB1 | DPB6 | DPB10 | DPB12 | DPB15 | DPB19 | DPB21 | 465 | |||
DPB1‡2101 | DPB2 | DPB6 | DPB10 | DPB12 | DPB16 | DPB19 | DPB21 | ||||
DPB1‡3601 | DPB2 | DPB6 | DPB10 | DPB12 | DPB15 | DPB19 | DPB21 | ||||
DPB1‡2401 | DPB1 | DPB3 | DPB10 | DPB12 | DPB15 | DPB19 | DPB22 | ||||
DPB1‡8401 | DPB1 | DPB5 | DPB11 | DPB12 | DPB15 | DPB18 | DPB22 | ||||
DPB1‡11011 | DPB2 | DPB4 | DPB7 | DPB13 | DPB17 | DPB19 | DPB21 | 467 | 473 | ||
DPB1‡11012 | DPB2 | DPB4 | DPB7 | DPB13 | DPB17 | DPB19 | DPB21 | 466 | 467 | 473 | |
DPB1‡1501 | DPB1 | DPB4 | DPB7 | DPB13 | DPB17 | DPB19 | DPB23 | 467 | 473 | ||
DPB1‡7401 | DPB2 | DPB4 | DPB7 | DPB13 | DPB17 | DPB19 | DPB23 | 467 | 473 | ||
DPB1‡3101 | DPB1 | DPB3 | DPB7 | DPB13 | DPB15 | DPB19 | DPB21 | ||||
DPB1‡5601 | DPB2 | DPB3 | DPB7 | DPB13 | DPB15 | DPB18 | DPB21 | ||||
DPB1‡7201 | DPB1 | DPB3 | DPB7 | DPB13 | DPB15 | DPB19 | DPB22 | ||||
DPB1‡3401 | DPB1 | DPB6 | DPB7 | DPB13 | DPB15 | DPB19 | DPB23 | 493 | |||
DPB1‡5201 | DPB2 | DPB5 | DPB7 | DPB13 | DPB15 | DPB18 | DPB21 | ||||
DPB1‡03012 | DPB2 | DPB5 | DPB8 | DPB13 | DPB15 | DPB18 | DPB21 | 482 | 503 | ||
DPB1‡1401 | DPB2 | DPB5 | DPB8 | DPB13 | DPB15 | DPB18 | DPB21 | 482 | 456 | ||
DPB1‡6101N | DPB2 | DPB5 | DPB8 | DPB13 | DPB15 | DPB18 | DPB2 | 482 | |||
DPB1‡7001 | DPB2 | DPB5 | DPB8 | DPB13 | DPB15 | DPB18 | DPB21 | 482 | 457 | ||
DPB1‡2501 | DPB2 | DPB5 | DPB8 | DPB13 | DPB15 | DPB18 | DPB21 | ||||
DPB1‡4501 | DPB2 | DPB5 | DPB8 | DPB13 | DPB15 | DPB18 | DPB21 | 456 | |||
DPB1‡6701 | DPB2 | DPB5 | DPB7 | DPB13 | DPB15 | DPB18 | DPB21 | 456 | |||
DPB1‡7801 | DPB2 | DPB5 | DPB8 | DPB13 | DPB15 | DPB18 | DPB21 | 482 | |||
DPB1‡2901 | DPB2 | DPB5 | DPB8 | DPB13 | DPB16 | DPB18 | DPB21 | 482 | |||
DPB1‡0601 | DPB2 | DPB5 | DPB8 | DPB13 | DPB16 | DPB19 | DPB21 | 482 | |||
DPB1‡20011 | DPB2 | DPB5 | DPB8 | DPB13 | DPB15 | DPB19 | DPB21 | 482 | |||
DPB1‡20012 | DPB2 | DPB5 | DPB8 | DPB13 | DPB15 | DPB19 | DPB21 | 482 | |||
DPB1‡6401N | DPB2 | DPB5 | DPB8 | DPB13 | DPB16 | DPB19 | DPB21 | ||||
DPB1‡6901 | DPB2 | DPB5 | DPB8 | DPB13 | DPB17 | DPB19 | DPB21 | 482 | |||
DPB1*5001 | DPB1 | DPB5 | DPB8 | DPB13 | DPB15 | DPB18 | DPB21 | 482 | |||
DPB1‡5701 | DPB1 | DPB5 | DPB8 | DPB13 | DPB15 | DPB18 | DPB21 | 482 | |||
DPB1‡5901 | DPB1 | DPB5 | DPB8 | DPB13 | DPB15 | DPB19 | DPB22 | ||||
DPB1‡7301 | DPB1 | DPB5 | DPB8 | DPB13 | DPB15 | DPB18 | DPB22 | ||||
DPB1‡8701 | DPB2 | DPB5 | DPB7 | DPB13 | DPB15 | DPB19 | DPB22 | ||||
DPB1‡2801 | DPB1 | DPB3 | DPB8 | DPB13 | DPB15 | DPB19 | DPB23 | ||||
DPB1‡7601 | DPB2 | DPB3 | DPB8 | DPB13 | DPB15 | DPB18 | DPB21 | 482 | 456 | ||
DPB1‡4401 | DPB2 | DPB6 | DPB8 | DPB13 | DPB16 | DPB18 | DPB21 | 482 | |||
DPB1‡03011 | DPB2 | DPB5 | DPB10 | DPB13 | DPB15 | DPB18 | DPB21 | 482 | |||
DPB1‡4101 | DPB1 | DPB5 | DPB8 | DPB14 | DPB16 | DPB19 | DPB22 | ||||
DPB1‡8301 | DPB1 | DPB5 | DPB8 | DPB14 | DPB15 | DPB19 | DPB22 | ||||
DPB1‡8601 | DPB2 | DPB5 | DPB8 | DPB9 | DPB16 | DPB19 | DPB22 | 482 |
Correspondence of Patchwork DPB1 and SEQ ID NOS. for DPB1 | |||
Patchwork | SEQ ID NOS. | ||
F | 458 | 460 | 462 |
DPB2 | 459 | 461 | 463 |
DPB3 | 468 | ||
DPB4 | 469 | 472 | |
DPB5 | 470 | ||
DPB6 | 471 | ||
DPB7 | 474 | ||
DPB8 | 475 | 479 | 480 |
DPB9 | 476 | ||
DPB10 | 477 | ||
DPB11 | 478 | ||
DPB12 | 483 | ||
DPB13 | 484 | 486 | |
DPB14 | 485 | ||
DPB15 | 489 | 92 | |
DPB16 | 490 | ||
DPB17 | 491 | ||
DPB18 | 496 | 498 | |
DPB19 | 497 | ||
DPB20 | 499 | ||
DPB21 | 500 | ||
DPB22 | 501 | ||
DPB23 | 502 |
Correspondence of the patchworks and SEQ ID Nos for DQB1 | ||||||
patchwork SEQ ID NO: | ||||||
DQB1 | 507 | |||||
DQB2 | 508 | |||||
DQB3 | 509 | |||||
DQB4 | 510 | |||||
DQB5 | 511 | |||||
DQB6 | 512 | |||||
DQB7 | 517 | 520 | 522 | |||
DQB8 | 518 | 521 | 523 | |||
DQB9 | 519 | |||||
DQB10 | 533 | 536 | 537 | |||
DQB11 | 534 | |||||
DQB12 | 535 | |||||
DQB13 | 540 | 543 | 544 | 545 | 546 | 547 |
DQB14 | 541 | |||||
DQB15 | 542 | |||||
DQB16 | 559 | |||||
DQB17 | 560 | |||||
DQB18 | 561 | 565 | 568 | 569 | ||
DQB19 | 562 | 566 | ||||
DQB20 | 563 | 567 | ||||
DQB21 | 564 | |||||
DQB22 | 572 | |||||
DQB23 | 573 | |||||
DQB24 | 574 | |||||
DQB25 | 575 | |||||
DQB26 | 576 | |||||
DQB27 | 577 | 579 | ||||
DQB28 | 578 | |||||
DQB29 | 586 | |||||
DQB30 | 587 | |||||
DQB31 | 588 | |||||
DQB32 | 589 | |||||
DQB33 | 528 | 532 | ||||
DQB34 | 554 | 557 | ||||
DQB35 | 555 | 558 |
Correspondence of the patchworks and SEQ ID Nos for DRB1 | |||||||||||||||
patchwork | SEQ ID NO: | ||||||||||||||
D1 | 600 | 603 | 604 | 605 | 606 | 607 | |||||||||
D2 | 601 | ||||||||||||||
D3 | 602 | ||||||||||||||
D4 | 609 | ||||||||||||||
D5 | 610 | 614 | 615 | 616 | 617 | ||||||||||
D6 | 611 | ||||||||||||||
D7 | 612 | ||||||||||||||
D8 | 613 | ||||||||||||||
D9 | 619 | 623 | 626 | 627 | |||||||||||
D10 | 620 | 624 | 627 | 629 | 630 | ||||||||||
D11 | 621 | 625 | |||||||||||||
D12 | 622 | ||||||||||||||
D13 | 633 | 643 | 650 | ||||||||||||
D14 | 635 | 645 | |||||||||||||
D15 | 637 | 647 | 653 | 657 | 661 | ||||||||||
D16 | 639 | 649 | |||||||||||||
D17 | 641 | ||||||||||||||
D18 | 634 | 644 | 651 | 655 | 659 | 663 | |||||||||
D19 | 636 | 646 | 652 | 656 | 660 | ||||||||||
D20 | 638 | 648 | 654 | 658 | 662 | ||||||||||
D21 | 640 | ||||||||||||||
D22 | 642 | ||||||||||||||
D23 | 667 | 673 | |||||||||||||
D24 | 668 | 674 | 678 | ||||||||||||
D25 | 669 | 675 | 679 | 681 | 682 | 683 | |||||||||
D26 | 670 | 676 | 680 | ||||||||||||
027 | 671 | 677 | |||||||||||||
D28 | 672 | ||||||||||||||
D29 | 689 | 691 | 693 | 695 | |||||||||||
D30 | 690 | 692 | 694 | ||||||||||||
D31 | 701 | ||||||||||||||
D32 | 702 | ||||||||||||||
D33 | 703 | ||||||||||||||
D34 | 704 | ||||||||||||||
D35 | 705 | ||||||||||||||
D36 | 706 | ||||||||||||||
D37 | 707 | ||||||||||||||
D38 | 708 | ||||||||||||||
D39 | 709 | ||||||||||||||
D40 | 710 | ||||||||||||||
D41 | 711 | ||||||||||||||
D42 | 712 | ||||||||||||||
D43 | 713 | 722 | 723 | 724 | |||||||||||
D44 | 714 | ||||||||||||||
D45 | 719 | ||||||||||||||
D46 | 720 | ||||||||||||||
D47 | 721 | ||||||||||||||
D48 | 726 | 729 | |||||||||||||
D49 | 727 | 730 | 732 | ||||||||||||
D50 | 728 | 731 | |||||||||||||
D51 | 733 | 741 | |||||||||||||
D52 | 734 | 742 | |||||||||||||
D53 | 735 | 743 | |||||||||||||
D54 | 736 | 744 | |||||||||||||
D55 | 737 | 745 | 747 | ||||||||||||
D56 | 738 | 746 | |||||||||||||
D57 | 739 | ||||||||||||||
D58 | 740 | ||||||||||||||
D59 | 748 | 754 | 756 | 758 | 759 | 760 | 761 | 762 | 763 | 764 | 765 | 767 | 768 | 769 | 770 |
D60 | 749 | 755 | 757 | ||||||||||||
D61 | 750 | ||||||||||||||
D62 | 752 | ||||||||||||||
D63 | 771 | 778 | 779 | 780 | 781 | ||||||||||
D64 | 772 | ||||||||||||||
D65 | 773 | ||||||||||||||
D66 | 774 | ||||||||||||||
D67 | 775 | ||||||||||||||
D68 | 776 | ||||||||||||||
D69 | 777 | ||||||||||||||
D70 | 784 | 787 | 788 | ||||||||||||
D71 | 785 | ||||||||||||||
D72 | 786 | ||||||||||||||
D73 | 792 | ||||||||||||||
D74 | 793 | 795 | 796 | 797 | 798 | ||||||||||
D75 | 794 | ||||||||||||||
D76 | 597 | 598 | 599 |
Correspondence of the patchwork regions and satellite regions for types of DRB 3-5 | |||||||||||||||||||
patchwork | satellite | ||||||||||||||||||
DRB3‡01011 | R1 | R3 | R7 | R17 | R19 | R21 | R25 | R27 | R31 | R32 | R34 | R40 | R43 | R45 | R50 | R52 | 832 | ||
DRB3‡0101201 | R1 | R3 | R7 | R17 | R19 | R21 | R25 | R27 | R31 | R32 | R34 | R40 | R43 | R45 | R50 | R52 | 832 | ||
DRB3‡0101202 | R1 | R3 | R7 | R17 | R19 | R21 | R25 | R27 | R31 | R32 | R34 | R40 | R43 | R45 | R50 | R52 | 832 | ||
DRB3‡01014 | R1 | R3 | R7 | R17 | R19 | R21 | R25 | R27 | R31 | R32 | R34 | R40 | R43 | R45 | R50 | R52 | 832 | 847 | |
DRB3‡0102 | R1 | R3 | R7 | R17 | R19 | R21 | R25 | R27 | R31 | R32 | R34 | R40 | R43 | R45 | R50 | R52 | 832 | 853 | |
DRB3‡0103 | R1 | R3 | R8 | R17 | R19 | R21 | R25 | R27 | R31 | R32 | R34 | R40 | R43 | R45 | R50 | R52 | 832 | ||
DRB3‡0104 | R1 | R3 | R7 | R17 | R19 | R21 | R25 | R27 | R31 | R32 | R34 | R40 | R43 | R45 | R50 | R52 | 832 | 852 | |
DRB3‡0105 | R1 | R3 | R10 | R17 | R19 | R21 | R25 | R27 | R31 | R32 | R34 | R40 | R43 | R45 | R50 | R52 | 832 | ||
DRB3‡0106 | R1 | R3 | R7 | R17 | R19 | R21 | R25 | R27 | R31 | R32 | R34 | R40 | R43 | R45 | R50 | R52 | |||
DRB3‡0209 | R1 | R2 | R8 | R16 | R19 | R21 | R24 | R27 | R31 | R32 | R34 | R40 | R43 | R46 | R50 | R52 | 832 | ||
DRB3‡0301 | R1 | R2 | R8 | R17 | R19 | R21 | R25 | R27 | R31 | R32 | R34 | R40 | R43 | R46 | R50 | R53 | |||
DRB3‡03012 | R1 | R2 | R8 | R17 | R19 | R21 | R25 | R27 | R31 | R32 | R34 | R40 | R43 | R46 | R50 | R53 | 832 | ||
DRB3‡0302 | R1 | R2 | R8 | R16 | R19 | R21 | R25 | R27 | R31 | R32 | R34 | R40 | R43 | R46 | R50 | R53 | |||
DRB3‡0303 | R1 | R2 | R8 | R17 | R19 | R21 | R25 | R27 | R31 | R32 | R34 | R40 | R43 | R45 | R50 | R52 | |||
DRB3‡01013 | R1 | R3 | R7 | R17 | R19 | R21 | R25 | R27 | R32 | R33 | R34 | R40 | R43 | R45 | R50 | R52 | 832 | ||
DRB3‡0107 | R1 | R3 | R7 | R17 | R19 | R21 | R24 | R27 | R28 | R34 | R40 | R43 | R46 | R50 | R52 | 834 | 847 | ||
DRB3‡02022 | R1 | R2 | R8 | R16 | R19 | R21 | R24 | R27 | R28 | R34 | R40 | R43 | R46 | R50 | R52 | 834 | 847 | ||
DRB3‡02010 | R1 | R2 | R8 | R16 | R19 | R21 | R24 | R27 | R28 | R34 | R40 | R43 | R46 | R50 | R52 | 832 | |||
DRB3‡02012 | R1 | R2 | R8 | R16 | R19 | R21 | R24 | R27 | R28 | R34 | R40 | R43 | R46 | R50 | R52 | 832 | 847 | ||
DRB3‡0205 | R1 | R2 | R8 | R17 | R19 | R21 | R24 | R27 | R28 | R34 | R40 | R43 | R46 | R50 | R52 | 832 | 847 | ||
DRB3‡02021 | R1 | R2 | R8 | R16 | R19 | R21 | R24 | R27 | R28 | R34 | R40 | R43 | R46 | R50 | R52 | 834 | 847 | ||
DRB3‡02011 | R1 | R2 | R8 | R16 | R19 | R21 | R24 | R27 | R28 | R35 | R40 | R43 | R46 | R50 | R52 | 832 | 847 | ||
DRB3‡02023 | R1 | R2 | R8 | R16 | R19 | R21 | R24 | R27 | R28 | R34 | R40 | R43 | R46 | R50 | R52 | 834 | 847 | 845 | |
DRB3‡0201 | R1 | R2 | R8 | R16 | R19 | R21 | R24 | R27 | R28 | R34 | R40 | R43 | R46 | R50 | R53 | 834 | 847 | ||
DRB3‡0203 | R1 | R2 | R8 | R16 | R19 | R21 | R22 | R27 | R28 | R34 | R40 | R43 | R46 | R50 | R52 | 847 | |||
DRB3‡0206 | R1 | R2 | R8 | R16 | R19 | R21 | R23 | R27 | R28 | R34 | R40 | R43 | R46 | R50 | R52 | 832 | 847 | ||
DRB3‡0204 | R1 | R2 | R8 | R16 | R19 | R21 | R24 | R27 | R28 | R34 | R40 | R43 | R45 | R50 | R52 | 832 | 847 | ||
DRB3‡02013 | R1 | R4 | R8 | R16 | R19 | R21 | R24 | R27 | R28 | R34 | R40 | R43 | R46 | R50 | R52 | 832 | 847 | ||
DRB3‡0207 | R1 | R2 | R8 | R16 | R19 | R21 | R24 | R27 | R31 | R34 | R40 | R43 | R46 | R50 | R52 | 832 | 847 | ||
DRB3‡0208 | R1 | R2 | R8 | R16 | R19 | R21 | R24 | R27 | R29 | R34 | R40 | R43 | R46 | R50 | R52 | 832 | 847 | ||
DRB4‡0103101 | R1 | R5 | R10 | R14 | R18 | R20 | R24 | R27 | R30 | R34 | R39 | R43 | R48 | R50 | R53 | ||||
DRB4‡01032 | R1 | R5 | R10 | R14 | R18 | R20 | R24 | R27 | R30 | R34 | R39 | R43 | R48 | R50 | R53 | ||||
DRB4‡01011 | R1 | R5 | R10 | R14 | R18 | R20 | R24 | R27 | R30 | R34 | R39 | R43 | R48 | R50 | R53 | ||||
DRB4‡01033 | R1 | R5 | R10 | R14 | R18 | R20 | R24 | R27 | R30 | R34 | R39 | R43 | R48 | R51 | R53 | ||||
DRB4‡0102 | R5 | R10 | R14 | R18 | R20 | R24 | R27 | R30 | R34 | R39 | R43 | R48 | R50 | R53 | 843 | ||||
DRB4‡0104 | R1 | R5 | R10 | R14 | R18 | R20 | R24 | R27 | R30 | R34 | R39 | R43 | R48 | R50 | R53 | 844 | |||
DRB4‡0105 | R1 | R5 | R10 | R14 | R18 | R20 | R24 | R27 | R30 | R34 | R39 | R43 | R48 | R50 | R53 | ||||
DRB4‡0201N | R1 | R6 | R11 | R14 | R18 | R20 | R24 | R27 | R30 | R34 | R39 | R43 | R48 | R50 | R53 | ||||
DRB5‡01011 | R1 | R2 | R12 | R15 | R18 | R20 | R26 | R27 | R30 | R36 | R38 | R44 | R49 | R50 | R52 | 833 | |||
DRB5‡01012 | R1 | R2 | R12 | R15 | R18 | R20 | R26 | R27 | R30 | R36 | R38 | R43 | R49 | R50 | R52 | 833 | |||
DRB5‡0105 | R1 | R2 | R12 | R15 | R18 | R20 | R26 | R27 | R30 | R36 | R38 | R44 | R49 | R50 | R52 | ||||
DRB5‡0104 | R1 | R2 | R12 | R15 | R18 | R20 | R26 | R27 | R30 | R36 | R38 | R43 | R47 | R50 | R52 | ||||
DRB5‡0106 | R1 | R2 | R12 | R15 | R18 | R20 | R26 | R27 | R30 | R35 | R41 | R43 | R49 | R50 | R53 | ||||
DRB5‡0107 | R1 | R2 | R12 | R15 | R18 | R20 | R26 | R27 | R30 | R35 | R38 | R44 | R49 | R50 | R52 | ||||
DRB5‡0109 | R1 | R2 | R12 | R15 | R18 | R20 | R26 | R27 | R30 | R36 | R42 | R44 | R49 | R50 | R52 | ||||
DRB5‡0108N | R1 | R2 | R12 | R13 | R18 | R20 | R23 | R27 | R30 | R36 | R38 | R44 | R49 | R50 | R52 | ||||
DRB5‡0102 | R1 | R2 | R12 | R13 | R18 | R20 | R23 | R27 | R30 | R36 | R38 | R44 | R49 | R50 | R52 | ||||
DRB5‡0110N | R1 | R2 | R12 | R13 | R18 | R20 | R23 | R27 | R30 | R36 | R38 | R44 | R49 | R50 | R52 | ||||
DRB5‡0103 | R1 | R2 | R12 | R13 | R18 | R20 | R23 | R27 | R30 | R36 | R31 | R44 | R49 | R50 | R52 | ||||
DRB5‡0202 | R1 | R2 | R12 | R13 | R18 | R20 | R23 | R27 | R30 | R35 | R41 | R43 | R49 | R50 | R53 | ||||
DRB5‡0203 | R1 | R2 | R12 | R13 | R18 | R20 | R23 | R27 | R30 | R35 | R41 | R43 | R49 | R50 | R52 | ||||
DRB5‡0204 | R1 | R2 | R12 | R13 | R18 | R20 | R23 | R27 | R30 | R36 | R41 | R43 | R49 | R50 | R53 | 848 | |||
DRB5‡0205 | R1 | R2 | R12 | R13 | R18 | R20 | R23 | R27 | R30 | R34 | R37 | R43 | R49 | R50 | R53 | 848 |
Correspondence of the patchworks and SEQ ID NOs for DRB 3-5 | |||||
Patchwork | SEQ ID NO: | ||||
R1 | 603 | 604 | 607 | ||
R2 | 610 | 803 | |||
R3 | 611 | 804 | 805 | ||
R4 | 800 | ||||
R5 | 801 | ||||
R6 | 802 | ||||
R7 | 620 | ||||
R8 | 621 | 810 | 812 | 813 | |
R9 | 806 | ||||
R10 | 807 | ||||
R11 | 808 | ||||
R12 | 809 | 811 | |||
R13 | 814 | ||||
R14 | 815 | ||||
R15 | 816 | ||||
R16 | 817 | ||||
R17 | 818 | 819 | 820 | ||
R18 | 643 | 821 | 822 | ||
R19 | 823 | 824 | |||
R20 | 825 | 826 | 659 | ||
R21 | 827 | 828 | 652 | 829 | |
R22 | 673 | ||||
R23 | 668 | 678 | |||
R24 | 682 | 831 | |||
R25 | 676 | 680 | |||
R26 | 830 | ||||
R27 | 689 | 691 | |||
R28 | 701 | ||||
R29 | 702 | ||||
R30 | 703 | ||||
R31 | 704 | ||||
R32 | 837 | ||||
R33 | 838 | ||||
R34 | 726 | ||||
R35 | 727 | 730 | |||
R36 | 728 | 731 | |||
R37 | 733 | ||||
R38 | 736 | 744 | 839 | ||
R39 | 737 | ||||
R40 | 746 | ||||
R41 | 739 | ||||
R42 | 1072 | ||||
R43 | 658 | 659 | 662 | 667 | 841 |
R44 | 655 | 840 | |||
R45 | 774 | ||||
R46 | 775 | ||||
R47 | 772 | 842 | |||
R48 | 773 | ||||
R49 | 771 | 780 | |||
R50 | 784 | 787 | 788 | ||
R51 | 785 | ||||
R52 | 792 | 850 | |||
R53 | 793 | 795 | 851 |
Correspondence of the patchworks and SEQ ID Nos for B | ||||||||||
patchwork | SEQ ID NO: | |||||||||
1B | 1080 | |||||||||
2B | 1081 | |||||||||
3B | 1082 | |||||||||
4B | 1083 | |||||||||
5B | 1084 | |||||||||
6B | 1085 | 1088 | ||||||||
7B | 1086 | |||||||||
8B | 1087 | |||||||||
9B | 1089 | 1094 | ||||||||
10B | 1090 | |||||||||
11B | 1091 | 1095 | ||||||||
12B | 1092 | 1096 | ||||||||
13B | 1093 | |||||||||
14B | 1097 | 1103 | 1106 | |||||||
15B | 1098 | 1104 | ||||||||
16B | 1099 | |||||||||
17B | 1100 | 1105 | 1107 | 1108 | ||||||
18B | 1101 | |||||||||
19B | 1102 | |||||||||
20B | 1109 | 1113 | 1115 | |||||||
21B | 1110 | 1114 | ||||||||
22B | 1111 | |||||||||
23B | 1112 | |||||||||
24B | 1116 | 1120 | ||||||||
25B | 1117 | 1121 | 1122 | |||||||
26B | 1118 | |||||||||
27B | 1119 | |||||||||
28B | 1123 | 1129 | 1133 | |||||||
29B | 1124 | 1130 | 1134 | 1135 | 1136 | 1137 | 1138 | 1139 | 1140 | 1141 |
30B | 1125 | 1131 | ||||||||
31B | 1126 | |||||||||
32B | 1127 | 1132 | ||||||||
33B | 1128 | |||||||||
34B | 1142 | 1149 | 1153 | 1156 | 1157 | |||||
35B | 1143 | 1150 | 1154 | |||||||
36B | 1144 | 1151 | ||||||||
37B | 1145 | |||||||||
38B | 1146 | |||||||||
39B | 1147 | |||||||||
40B | 1148 | 1152 | 1155 | |||||||
41B | 1158 | 1162 | ||||||||
42B | 1159 | |||||||||
43B | 1160 | 1163 | 1164 | |||||||
44B | 1161 | |||||||||
45B | 1165 | |||||||||
46B | 1166 | |||||||||
47B | 1167 | 1169 | ||||||||
48B | 1168 | |||||||||
49B | 1170 | 1179 | ||||||||
50B | 1175 | 1180 | ||||||||
51B | 1183 | 1212 | 1218 | 1222 | ||||||
528 | 1195 | 1213 | 1219 | 1223 | 1226 | |||||
53B | 1206 | 1215 | ||||||||
54B | 1209 | 1216 | 1220 | 1224 | ||||||
55B | 1211 | 1217 | 1221 | 1225 | ||||||
56B | 1227 | 1246 | ||||||||
57B | 1229 | 1247 | 1251 | 1253 | 1255 | 1256 | ||||
58B | 1239 | 1249 | 1252 | 1254 | ||||||
59B | 1242 | 1250 | ||||||||
60B | 1259 | 1274 | 1277 | |||||||
61B | 1261 | 1275 | 1278 | 1279 | 1280 | 1281 | 1282 | 1283 | 1284 | |
62B | 1269 | 1276 | ||||||||
63B | 1288 | 1295 | ||||||||
64B | 1296 | 1299 |
Correspondence of Primers | ||
SEQ ID NOS. | SEQ ID NOS. | |
Primers for DRB1 | 590, 591, 591 | 593 |
Primers for DPB1 | 448, 449 | 450-455 |
Primers for DQB1 | 504, 505 | 506 |
Primers for A | 899, 900, 903-905 | 901, 902, 906, 907 |
Primers for B | 1073, 1075-1077 | 1074, 1078, 1079 |
[References]
(1) Kobayashi, T. et al.: The significance of HLA-DRB1 matching in clinical renal transplantation, Transplantation, 54: 238, 1992
(2) Olerup, O. et al.: HLA-DR typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours: An alternative to serological DR typing in clinical practice including donor-recipient matching in cadaveric transplantation, Tissue Antigen, 39: 225, 1992
(3) Inoko, H. et al.: PCR-RFLP, Handbook of HLA typing techniques (edited by Hui, K.M. & Bidwell, J.L.), CRC press, Florida, U.S.A., 1993, p.9
(4) Erlich, H. et al.: HLA-DR, -DQ and -DP typing using PCR amplification and immobilized probes, European Journal of Immunogenetics, 18: 33, 1991
(5) Carrington, M. et al.: Typing of HLA-DQA1 and DQB1 using DNA single-strand conformation polymorphism, Hum. Immunol., 33: 208, 1992
Industrial Applicability
carrying out low accuracy typing of HLA genotype of the specimen by performing first PCR amplification using the primers for low accuracy genotyping according to claim 21 and a nucleic acid sequence derived from the specimen as a template, allowing hybridization of the amplification product with the oligonucleotides contained in the typing kit according to any one of claims 1-16, and detecting occurrence of hybridization of the nucleic acid sequence derived from the specimen and each of the oligonucleotides; and
carrying out high accuracy HLA genotyping of the specimen by performing second PCR amplification based on the above determination result using primers appropriately selected from the primers for high accuracy genotyping according to claim 22 and a nucleic acid sequence derived from the specimen as a template, allowing hybridization of the amplification product with the oligonucleotides contained in the typing kit according to any one of claims 1-16, and detecting occurrence of hybridization of the nucleic acid sequence derived from the specimen and each of the oligonucleotides.