Methods of amplifying and sequencing nucleic acids

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EP 2 159 285 A3

(12)	EUROPEAN PATENT APPLICATION

(88)	Date of publication A3:
	29.06.2011 Bulletin 2011/26

(43)	Date of publication A2:
	03.03.2010 Bulletin 2010/09

(21)	Application number: 09166658.6

(22)	Date of filing: 28.01.2004

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International Patent Classification (IPC):

C12N 15/10^(2006.01)

C12Q 1/68^(2006.01)

(84)	Designated Contracting States:
	AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR

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Priority:

29.01.2003 US 443471 P
23.04.2003 US 465071 P
06.06.2003 US 476602 P
06.06.2003 US 476504 P
06.06.2003 US 476313 P
06.06.2003 US 476592 P
25.08.2003 US 497985 P

(60)	Divisional application:
	10179652.2 / 2261372

(62)	Application number of the earlier application in accordance with Art. 76 EPC:
	04785758.6 / 1590477

(71)	Applicant: 454 Life Sciences Corporation
	Branford CT 06405 (US)

(72)	Inventors:
	Leamon, John H. Guilford, CT 06437 (US) Lohman, Kenton Guilford, CT 06437 (US) Rothberg, Jonathan M. Guilford, CT 06437 (US) Weiner, Michael P. Guilford, CT 06437 (US)

(74)	Representative: Aston, Heidi Frances
	Mintz, Levin, Cohn, Ferris, Glovsky and Popeo IP, LLP Alder Castle 10 Noble Street London EC2V 7JX London EC2V 7JX (GB)

(54)	Methods of amplifying and sequencing nucleic acids

(57) An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.

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