Technical Field
[0002] The present disclosure relates to a cyclic di-nucleotide analogue, a pharmaceutical
composition thereof and a use thereof as STING agonist and for activation of the STING
pathway.
Background
[0003] Stimulator of Interferon Genes (STING), also known as T1MEM173, MITA, MPYS and ERIS,
is an important signaling molecule in innate immune signaling. The protein encoded
by this gene contains a 5-stranded transmembrane structure and plays an important
regulatory role in the immune response associated with viral or bacterial infection.
STING is a pattern recognition receptor which detects exogenous nucleic acids in the
cytoplasm and activates signal transduction pathways associated with type I interferon
responses. In addition, it has been shown that STING is involved in the regulation
of apoptotic signaling by interacting with the type II major histocompatibility complex
(MHCII). Studies performed on human tumors with spontaneous T-cell infiltration have
shown that CD8+ T-cell infiltration is closely associated with the transcriptional
profile of type I interferons (
Harlin et al., Cancer Res, 2009; 69(7): OF1). Mechanistic studies carried out on mouse models have shown that T cell activation
processes against tumor-associated antigens show abnormalities in experimental animals
with defective type I interferon signaling (
Diamond et al., J. Exp. Med., 2011; 208(10): 1989;
Fuerte et al., J. Exp. Med, 2011; 208(10):2005). Further studies on tumor recognition by the innate immune system
in vivo and on the signaling pathways involved in this process, such as tumor-triggered IFN
expression mediated by antigen-presenting cells (APCs), have revealed that the STING
signaling pathway can be activated by cytoplasmic DNA, and that these exogenous nucleic
acids can be recognized by cyclic-GMP-AMP synthetase (cGAS) and then catalyzes the
generation of cyclized nucleic acids such as cyclic GMP-AMP (cGMP) which can act as
endogenous ligands activating STING signaling (
Sun et al, Science, 2013; 339(15): 786). Activated STING can subsequently induce autophosphorylation of TBK1 kinase and
phosphorylation of interferon regulatory factor 3 (IRF-3), and phosphorylated IRF3
can further activate the gene transcription process of type I interferon and regulate
the synthesis and secretion of type I interferon, which in turn induces an immune
response. In summary, it has been shown that the STING signaling pathway plays an
extremely important role in the tumor recognition process by the innate immune system,
and the activation of this signaling pathway on antigen-presenting cells is directly
related to the activation of T cells against tumor-associated antigens. Based on its
role in tumor immune recognition, it can be expected that activation of STING signaling
by drugs or other pharmacological approaches can enhance IFN expression and have a
positive effect on tumor therapy. Therefore, the development of STING signaling agonists
for the treatment of tumor diseases has become a hot research topic.
[0004] In addition, it has been shown that the stimulation of STING signaling pathway activation
also contributes to antiviral responses. Loss of functional response at the cellular
or organismal level demonstrates that viral load cannot be controlled in the absence
of STING. Activation of the STING signaling pathway triggers immune response leading
to anti-vascular and pro-inflammatory cytokines against the virus and mobilizes the
innate and acquired immune systems. Thus, small molecule compounds with agonistic
effects on the STING signaling pathway have potential for the treatment of chronic
viral infections and could be used, for example, to treat HBV.
[0005] Several cyclic di-nucleotide analogs with agonistic effects on the STING signaling
pathway have been disclosed (
WO2014/093936,
WO2014/189805,
WO2014/189806,
WO2016/120305,
WO2016/145102,
WO2017/027645,
WO2017/075477,
WO2017/093933,
WO2017/123657,
WO2017/123669,
WO2017/161349,
WO2017/186711,
WO2018/009466,
WO2018/009648,
WO2018/009652,
WO2018/ 045204,
WO2018/060323,
WO2018/065360,
WO2018/098203,
WO2018/100558), but at present, no STING agonist has been approved for marketing.
Content of the present disclosure
[0006] The technical problem to be solved in the present disclosure is to provide a novel
cyclic di-nucleotide analogue, a pharmaceutical composition thereof and a use thereof.
The cyclic di-nucleotide analogue of the present disclosure has good STING modulating
effect and can effectively treat, alleviate and/or prevent various diseases caused
by immunosuppression, such as tumors, infectious diseases, neurodegenerative diseases,
psychiatric disorders or autoimmune diseases.
[0007] The present disclosure provides a cyclic di-nucleotide analogue (I), an isomer, a
prodrug, a stable isotope derivative or a pharmaceutically acceptable salt thereof;
wherein, each of Z1, Z2 is independently O, S, SO2, CH2, CF2 or Se;
B1 is


B2 is




L and L1 are each independently a connecting bond or CR1R2;
L' and L1' are each independently a connecting bond or CR11R21;
L2 is O, S or CR3R4; L2' is O, S or CR31R41;
X1 is O, S or CR5R6; X11 is O, S or CR51R61;
X2 is O, S, or CR7R8; and X21 is O, S, or CR71R81;
X3 and X31 are each independently OH, SH or BH3-;
R and R' are each independently hydrogen, C2-6 alkenyl, C2-6 alkynyl or C1-6 alkyl; the C2-6 alkenyl, C2-6 alkynyl or C1-6 alkyl is unsubstituted or selectively substituted at any position by 1 to 3 substituents
selected from halogen, hydroxyl, amino, azido and cyano;
R1 and R2 are each independently hydrogen, halogen, cyano, hydroxyl, thiol, amino, azido, C2-6 alkenyl, C2-6 alkynyl, C1-6 alkyl, halo-C1-6 alkyl, halo-C1-6 alkoxy, halo-C1-6 alkylthio, C1-6 alkylamino, OC(O)Ra or ORa; the C2-6 alkenyl, C2-6 alkynyl, or C1-6 alkyl is unsubstituted or selectively substituted at any position by 1 to 3 substituents
selected from halogen, hydroxyl, amino, azido, and cyano;
R11 and R21 are each independently hydrogen, halogen, cyano, hydroxyl, thiol, amino, azido, C2-6 alkenyl, C2-6 alkynyl, C1-6 alkyl, halo-C1-6 alkyl, halo-C1-6 alkoxy, halo-C1-6 alkylthio, C1-6 alkylamino, OC(O)Ra, or ORa; the C2-6 alkenyl, C2-6 alkynyl, or C1-6 alkyl is unsubstituted or selectively substituted at any position by 1 to 3 substituents
selected from halogen, hydroxyl, amino, azido, and cyano;
alternatively, R1 and R2 together form carbonyl;
alternatively, R11 and R21 together form carbonyl;
alternatively, R is -CH2-, R1 is -O-, R and R1 are interconnected to form heterocycloalkyl;
alternatively, R' is -CH2-, R11 is -O-, R' and R11 are interconnected to form heterocycloalkyl;
R3 and R4 are each independently hydrogen, deuterium, halogen or C1-6 alkyl;
R31 and R41 are each independently hydrogen, deuterium, halogen or C1-6 alkyl;
R5 and R6 are each independently hydrogen, deuterium, halogen or C1-6 alkyl;
R51 and R61 are each independently hydrogen, deuterium, halogen or C1-6 alkyl;
R7 and R8 are each independently hydrogen, deuterium, halogen or C1-6 alkyl;
R71 and R81 are each independently hydrogen, deuterium, halogen or C1-6 alkyl;
Y and Y1 are each independently CRE or N;
U is CHRE' or NRD';
U1 is CH or N;
V, V1, V2 and V3 are each independently CRE" or N;
W is O or S;
W1, W2, W3 and W4 are each independently N or CRF';
each of RA, RB, RC, RE, RE', RE", RF, RF' and RG is independently H, halogen, -CN, - NO2, -N3, Rc, -SRc, -ORc, -OC(O)Rc, -OC(O)ORc, -OC(O)NRbRc, -C(O)ORc, -C(O)Rc, - C(O)NRbRc, -NRbRc, -NRbC(O)Rc, -N(Rb)C(O)ORc, -N(Ra)C(O)NRbRc, -NRbS(O)2Rc, - NRbC(=NH)Rc, -NRbC(=NRc)NH2, -S(O)1-2Rc, -S(O)2NRbRc or -NRaS(O)2NRbRc;
each of RD and RD' is independently H or Rc;
each of Ra and Rb is independently H, C2-6 alkenyl, C2-6 alkynyl, C1-10 alkyl, halo-C1-6 alkyl, aryl, heteroaryl, cycloalkyl, heterocycloalkyl, aryl-C1-6 alkyl, heteroaryl-C1-6 alkyl, cycloalkyl-C1-6 alkyl, or heterocycloalkyl-C1-6 alkyl;
each Rc is independently H, substituted or unsubstituted C1-10 alkyl, substituted or unsubstituted C2-8 alkenyl, substituted or unsubstituted C2-8 alkynyl, substituted or unsubstituted C3-10 cycloalkyl, substituted or unsubstituted C6-10 aryl, substituted or unsubstituted 3-10 membered heterocycloalkyl, substituted or
unsubstituted 5-10 membered heteroaryl, substituted or unsubstituted C6-10 aryl-C1-6 alkyl, substituted or unsubstituted C3-10 cycloalkyl-C1-6 alkyl, substituted or unsubstituted 3-10 membered heterocycloalkyl-C1-6 alkyl, substituted or unsubstituted 5-10 membered heteroaryl-C1-6 alkyl; the C1-10 alkyl, C2-8 alkenyl, C2-8 alkynyl, C3-10 cycloalkyl, C6-10 aryl, 3-10 membered heterocycloalkyl, 5-10 membered heteroaryl, C6-10 aryl-C1-6 alkyl, C3-10 cycloalkyl-C1-6 alkyl, 3-10 membered heterocycloalkyl-C1-6 alkyl, or 5-10 membered heteroaryl-C1-6 alkyl is unsubstituted or selectively substituted at any position by one or more
Rd;
each Rd is independently halogen, halo-C1-6 alkyl, halo-C1-6 alkoxy, C1-6 alkyl, -CN, - N3, -SRe, -ORe, -C(O)Re, -NReRe', substituted or unsubstituted C6-10 aryl, substituted or unsubstituted 5-10 membered heteroaryl, substituted or unsubstituted
C3-10 cycloalkyl, or substituted or unsubstituted 3-10 membered heterocycloalkyl; the C6-10 aryl, 5-10 membered heteroaryl, C3-10 cycloalkyl or 3-10 membered heterocycloalkyl is unsubstituted or selectively substituted
at any position by one or more substituents selected from halogen, hydroxyl, cyano,
amino, C1-4 alkyl, halo-C1-4 alkyl, C1-4 alkoxy, C1-4 alkylamino and halo-C1-4 alkoxy;
each of Re and Re' is independently C2-6 alkenyl, C2-6 alkynyl, C1-10 alkyl, halo-C1-6 alkyl, aryl, heteroaryl, cycloalkyl, heterocycloalkyl, aryl-C1-6 alkyl, heteroaryl-C1-6 alkyl, cycloalkyl-C1-6 alkyl, or heterocycloalkyl-C1-6 alkyl.
[0008] In some embodiments, the cyclic di-nucleotide analogue (I), the isomer, prodrug,
stable isotope derivative or pharmaceutically acceptable salt, having the structural
general formula of
wherein, Z1, Z2 are each independently O, S, SO2, CH2, CF2 or Se;
B1 is

B2 is




L and L1 are each independently a connecting bond or CR1R2;
L' and L1' are each independently a connecting bond or CR11R21;
L2 is O, S or CR3R4; L2' is O, S or C R31R41;
X1 is O, S or CR5R6; X11 is O, S or CR51R61;
X2 is O, S or CR7R8; and X21 is O, S or CR71R81;
X3 and X31 are each independently OH, SH or BH3-;
R and R' are each independently hydrogen, C2-6 alkenyl, C2-6 alkynyl or C1-6 alkyl; the C2-6 alkenyl, C2-6 alkynyl or C1-6 alkyl is unsubstituted or selectively substituted at any position by 1 to 3 substituents
selected from halogen, hydroxyl, amino, azido and cyano;
R1 and R2 are each independently hydrogen, halogen, cyano, hydroxyl, thiol, amino, azido, C2-6 alkenyl, C2-6 alkynyl, C1-6 alkyl, halo-C1-6 alkyl, halo-C1-6 alkoxy, halo-C1-6 alkylthio, C1-6 alkylamino, OC(O)Ra or ORa; the C2-6 alkenyl, C2-6 alkynyl, or C1-6 alkyl is unsubstituted or selectively substituted at any position by 1 to 3 substituents
selected from halogen, hydroxyl, amino, azido, and cyano;
R11 and R21 are each independently hydrogen, halogen, cyano, hydroxyl, thiol, amino, azido, C2-6 alkenyl, C2-6 alkynyl, C1-6 alkyl, halo-C1-6 alkyl, halo-C1-6 alkoxy, halo-C1-6 alkylthio, C1-6 alkylamino, OC(O)Ra or ORa; the C2-6 alkenyl, C2-6 alkynyl, or C1-6 alkyl is unsubstituted or selectively substituted at any position by 1 to 3 substituents
selected from halogen, hydroxyl, amino, azido, and cyano;
alternatively, R1 and R2 together form carbonyl;
alternatively, R11 and R21 together form carbonyl;
alternatively, R is -CH2-, R1 is -O-, R and R1 are interconnected to form heterocycloalkyl;
alternatively, R' is -CH2-, R11 is -O-, R' and R11 are interconnected to form heterocycloalkyl;
R3 and R4 are each independently hydrogen, deuterium, halogen or C1-6 alkyl;
R31 and R41 are each independently hydrogen, deuterium, halogen or C1-6 alkyl;
R5 and R6 are each independently hydrogen, deuterium, halogen or C1-6 alkyl;
R51 and R61 are each independently hydrogen, deuterium, halogen or C1-6 alkyl;
R7 and R8 are each independently hydrogen, deuterium, halogen or C1-6 alkyl;
R71 and R81 are each independently hydrogen, deuterium, halogen or C1-6 alkyl;
Y and Y1 are each independently CRE or N;
U is CHRE' or NRD';
U1 is CH or N;
V and V1 are each independently CRE" or N;
W is O or S;
each of RA, RB, RC, RE, RE', RE", RF and RG is independently H, halogen, -CN, -NO2, -N3, Rc, -SRc, -ORc, -OC(O)Rc, -OC(O)ORc, -OC(O)NRbRc, -C(O)ORc, -C(O)Rc, -C(O)NRbRc, -NRbRc, -NRbC(O)Rc, -N(Rb)C(O)ORc, -N(Ra)C(O)NRbRc, -NRbS(O)2Rc, -NRbC(=NH)Rc, - NRbC(=NRc)NH2, -S(O)1-2Rc, -S(O)2NRbRc or -NRaS(O)2NRbRc;
each of RD and RD' is independently H or Rc;
each of Ra and Rb is independently H, C2-6 alkenyl, C2-6 alkynyl, C1-10 alkyl, halo-C1-6 alkyl, aryl, heteroaryl, cycloalkyl, heterocycloalkyl, aryl-C1-6 alkyl, heteroaryl-C1-6 alkyl, cycloalkyl-C1-6 alkyl, or heterocycloalkyl-C1-6 alkyl;
each Rc is independently H, substituted or unsubstituted C1-10 alkyl, substituted or unsubstituted C2-8 alkenyl, substituted or unsubstituted C2-8 alkynyl, substituted or unsubstituted C3-10 cycloalkyl, substituted or unsubstituted C6-10 aryl, substituted or unsubstituted 3-10 membered heterocycloalkyl, substituted or
unsubstituted 5-10 membered heteroaryl, substituted or unsubstituted C6-10 aryl-C1-6 alkyl, substituted or unsubstituted C3-10 cycloalkyl-C1-6 alkyl, substituted or unsubstituted 3-10 membered heterocycloalkyl-C1-6 alkyl, substituted or unsubstituted 5-10 membered heteroaryl-C1-6 alkyl; the C1-10 alkyl, C2-8 alkenyl, C2-8 alkynyl, C3-10 cycloalkyl, C6-10 aryl, 3-10 membered heterocycloalkyl, 5-10 membered heteroaryl, C6-10 aryl-C1-6 alkyl, C3-10 cycloalkyl-C1-6 alkyl, 3-10 membered heterocycloalkyl-C1-6 alkyl, or 5-10 membered heteroaryl-C1-6 alkyl is unsubstituted or selectively substituted at any position by one or more
Rd;
each Rd is independently halogen, halo-C1-6 alkyl, halo-C1-6 alkoxy, C1-6 alkyl, -CN, - N3, -SRe, -ORe, -C(O)Re, -NReRe', substituted or unsubstituted C6-10 aryl, substituted or unsubstituted 5-10 membered heteroaryl, substituted or unsubstituted
C3-10 cycloalkyl, or substituted or unsubstituted 3-10 membered heterocycloalkyl; the C6-10 aryl, 5-10 membered heteroaryl, C3-10 cycloalkyl or 3-10 membered heterocycloalkyl is unsubstituted or selectively substituted
at any position by one or more substituents selected from halogen, hydroxyl, cyano,
amino, C1-4 alkyl, halo-C1-4 alkyl, C1-4 alkoxy, C1-4 alkylamino and halo-C1-4 alkoxy;
each of Re and Re' is independently C2-6 alkenyl, C2-6 alkynyl, C1-10 alkyl, halo-C1-6 alkyl, aryl, heteroaryl, cycloalkyl, heterocycloalkyl, aryl-C1-6 alkyl, heteroaryl-C1-6 alkyl, cycloalkyl-C1-6 alkyl, or heterocycloalkyl-C1-6 alkyl.
[0009] In some embodiments, the cyclic di-nucleotide analogue (I), the isomer, prodrug,
stable isotope derivative or pharmaceutically acceptable salt thereof, having the
structural general formula of
wherein, Z1, Z2 are each independently O, S, SO2, CH2, CF2 or Se;
B1 is

B2 is




L and L1 are each independently a connecting bond or CR1R2;
L' and L1' are each independently a connecting bond or CR11R21;
L2 is O, S or CR3R4; L2' is O, S or CR31R41;
X1 is O, S or CR5R6; X11 is O, S or CR51R61;
X2 is O, S or CR7R8; and X21 is O, S or CR71R81;
X3 and X31 are each independently OH, SH or BH3-;
R and R' are each independently hydrogen, C2-6 alkenyl, C2-6 alkynyl or C1-6 alkyl; the C2-6 alkenyl, C2-6 alkynyl or C1-6 alkyl is unsubstituted or selectively substituted at any position by 1 to 3 substituents
selected from halogen, hydroxyl, amino, azido and cyano;
R1 and R2 are each independently hydrogen, halogen, cyano, hydroxyl, thiol, amino, azido, C2-6 alkenyl, C2-6 alkynyl, C1-6 alkyl, halo-C1-6 alkyl, halo-C1-6 alkoxy, halo-C1-6 alkylthio, C1-6 alkylamino, OC(O)Ra, or ORa; the C2-6 alkenyl, C2-6 alkynyl, or C1-6 alkyl is unsubstituted or selectively substituted at any position by 1 to 3 substituents
selected from halogen, hydroxyl, amino, azido, and cyano;
R11 and R21 are independently hydrogen, halogen, cyano, hydroxyl, thiol, amino, azido, C2-6 alkenyl,
C2-6 alkynyl, C1-6 alkyl, halo-C1-6 alkyl, halo-C1-6 alkoxy, halo-C1-6 alkylthio, C1-6 alkylamino, OC(O)Ra, or ORa; the C2-6 alkenyl, C2-6 alkynyl, or C1-6 alkyl is unsubstituted or selectively substituted at any position by 1 to 3 substituents
selected from halogen, hydroxyl, amino, azido, and cyano;
alternatively, R1 and R2 together form carbonyl;
alternatively, R11 and R21 together form carbonyl;
alternatively, R is -CH2-, R1 is -O-, R and R1 are interconnected to form heterocycloalkyl;
alternatively, R' is -CH2-, R11 is -O-, R' and R11 are interconnected to form heterocycloalkyl;
R3 and R4 are each independently hydrogen, deuterium, halogen or C1-6 alkyl;
R31 and R41 are each independently hydrogen, deuterium, halogen or C1-6 alkyl;
R5 and R6 are each independently hydrogen, deuterium, halogen or C1-6 alkyl;
R51 and R61 are each independently hydrogen, deuterium, halogen or C1-6 alkyl;
R7 and R8 are each independently hydrogen, deuterium, halogen or C1-6 alkyl;
R71 and R81 are each independently hydrogen, deuterium, halogen or C1-6 alkyl;
Y and Y1 are each independently CRE or N;
U is CHRE' or NRD';
U1 is CH or N;
V and V1 are each independently CRE" or N;
W is O or S;
each of RA, RB, RC, RE, RE', RE", RF and RG is independently H, halogen, -CN, -NO2, -N3, Rc, -SRc, -ORc, -OC(O)Rc, -OC(O)ORc, -OC(O)NRbRc, -C(O)ORc, -C(O)Rc, -C(O)NRbRc, -NRbRc, -NRbC(O)Rc, -N(Rb)C(O)ORc, -N(Ra)C(O)NRbRc, -NRbS(O)2Rc, -NRbC(=NH)Rc, - NRbC(=NRc)NH2, -S(O)1-2Rc, -S(O)2NRbRc or -NRaS(O)2NRbRc;
each of RD and RD' is independently H or Rc;
each of Ra and Rb is independently H, C2-6 alkenyl, C2-6 alkynyl, C1-10 alkyl, halo-C1-6 alkyl, aryl, heteroaryl, cycloalkyl, heterocycloalkyl, aryl-C1-6 alkyl, heteroaryl-C1-6 alkyl, cycloalkyl-C1-6 alkyl, or heterocycloalkyl-C1-6 alkyl;
each Rc is independently H, substituted or unsubstituted C1-10 alkyl, substituted or unsubstituted C2-8 alkenyl, substituted or unsubstituted C2-8 alkynyl, substituted or unsubstituted C3-10 cycloalkyl, substituted or unsubstituted C6-10 aryl, substituted or unsubstituted 3-10 membered heterocycloalkyl, substituted or
unsubstituted 5-10 membered heteroaryl, substituted or unsubstituted C6-10 aryl-C1-6 alkyl, substituted or unsubstituted C3-10 cycloalkyl-C1-6 alkyl, substituted or unsubstituted 3-10 membered heterocycloalkyl-C1-6 alkyl, substituted or unsubstituted 5-10 membered heteroaryl-C1-6 alkyl; the C1-10 alkyl, C2-8 alkenyl, C2-8 alkynyl, C3-10 cycloalkyl, C6-10 aryl, 3-10 membered heterocycloalkyl, 5-10 membered heteroaryl, C6-10 aryl-C1-6 alkyl, C3-10 cycloalkyl-C1-6 alkyl, 3-10 membered heterocycloalkyl-C1-6 alkyl, or 5-10 membered heteroaryl-C1-6 alkyl is unsubstituted or selectively substituted at any position by one or more
Rd;
each Rd is independently halogen, halo-C1-6 alkyl, halo-C1-6 alkoxy, C1-6 alkyl, -CN, - N3, -SRe, -ORe, -C(O)Re, -NReRe', substituted or unsubstituted C6-10 aryl, substituted or unsubstituted 5-10 membered heteroaryl, substituted or unsubstituted
C3-10 cycloalkyl, or substituted or unsubstituted 3-10 membered heterocycloalkyl; the C6-10 aryl, 5-10 membered heteroaryl, C3-10 cycloalkyl or 3-10 membered heterocycloalkyl is unsubstituted or selectively substituted
at any position by one or more substituents selected from halogen, hydroxyl, cyano,
amino, C1-4 alkyl, halo-C1-4 alkyl, C1-4 alkoxy, C1-4 alkylamino and halo-C1-4 alkoxy;
each of Re and Re' is independently C2-6 alkenyl, C2-6 alkynyl, C1-10 alkyl, halo-C1-6 alkyl, aryl, heteroaryl, cycloalkyl, heterocycloalkyl, aryl-C1-6 alkyl, heteroaryl-C1-6 alkyl, cycloalkyl-C1-6 alkyl, or heterocycloalkyl-C1-6 alkyl.
[0010] In some embodiments, each R
c is independently H, substituted or unsubstituted C
1-10 alkyl, substituted or unsubstituted C
3-10 cycloalkyl, substituted or unsubstituted C
6-10 aryl, substituted or unsubstituted 3-10 membered heterocycloalkyl, substituted or
unsubstituted 5-10 membered heteroaryl, substituted or unsubstituted C
6-10 aryl-C
1-6 alkyl, substituted or unsubstituted C
3-10 cycloalkyl-C
1-6 alkyl, substituted or unsubstituted 3-10 membered heterocycloalkyl-C
1-6 alkyl, substituted or unsubstituted 5-10 membered heteroaryl-C
1-6 alkyl; the C
1-10 alkyl, C
3-10 cycloalkyl, C
6-10 aryl, 3-10 membered heterocycloalkyl, 5-10 membered heteroaryl, C
6-10 aryl-C
1-6 alkyl, C
3-10 cycloalkyl-C
1-6 alkyl, 3-10 membered heterocycloalkyl-C
1-6 alkyl or 5-10 membered heteroaryl-C
1-6 alkyl is unsubstituted or selectively substituted at any position by one or more
R
d; R
d is as defined above.
[0011] In the definition of R
c, the substituted or unsubstituted C
1-10 alkyl is preferably substituted or unsubstituted C
1-6 alkyl, such as methyl, ethyl, propyl, isopropyl,
tert-butyl, n-butyl; wherein the methyl, ethyl, propyl, isopropyl,
tert-butyl, or n-butyl is substituted or unsubstituted;
in the definition of R
c, the substituted or unsubstituted C
3-10 cycloalkyl is preferably cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, 2,3-dihydro-1
H-inden-1-yl, 2,3-dihydro-1
H-inden-2-yl; wherein the cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, 2,3-dihydro-1
H-inden-1-yl, or 2,3-dihydro-1
H-inden-2-yl is substituted or unsubstituted;
in the definition of R
c, the substituted or unsubstituted C
6-10 aryl is preferably substituted or unsubstituted phenyl;
in the definition of R
c, the substituted or unsubstituted 3-10 membered heterocycloalkyl is preferably tetrahydrofuran-3-yl,
tetrahydro-2
H-pyran-3-yl, tetrahydro-2
H-pyran-4-yl, pyrrolidin-3-yl, piperidin-3-yl, piperidin-4-yl; wherein the tetrahydrofuran-3-yl,
tetrahydro-2
H-pyran-3-yl, tetrahydro-2
H-pyran-4-yl, pyrrolidin-3-yl, piperidin-3-yl, piperidin-4-yl is substituted or unsubstituted;
in the definition of R
c, the substituted or unsubstituted 5-10 membered heteroaryl is preferably substituted
or unsubstituted 5-6-membered heteroaryl.
[0012] In some embodiments, each R
c is independently H, C
1-4 alkyl or halo-C
1-4 alkyl.
[0013] In some embodiments, each R
b is independently H or C
1-4 alkyl.
[0014] In some embodiments, Z
1 is O.
[0015] In some embodiments, Z
2 is O.
[0016] In some embodiments, in the definition of B-4 to B-16, Y is N.
[0017] In some embodiments, in the definition of B-4 to B-16, Y is CR
E, and R
E is preferably H, F, Cl, -CF
3, -CH
3, -CN, -NH
2.
[0018] In some embodiments, in the definition of B-4 to B-16, Y
1 is N.
[0019] In some embodiments, in the definition of B-4 to B-16, Y
1 is CR
E, and R
E is preferably H, F, Cl, -CF
3, -CH
3, -CN or -NH
2.
[0020] In some embodiments, in the definition of B-6 to B-9, B-13, B-16 to B-17, U is NR
D' and R
D' is H or -CH
3.
[0021] In some embodiments, in the definition of B-1 to B-5, B-10 to B-13, B-15, V is N
or CH.
[0022] In some embodiments, in the definition of B-1 to B-12, B-17, V
1 is N or CH.
[0023] In some embodiments, in the definition of B-1 to B-3, R
A is H, halogen, -OR
c, -NR
bR
c; wherein R
b and R
c are as defined above.
[0024] In some embodiments, in the definition of B-4, B-5, B-10 to B-12, B-15, R
B is H, halogen, -OR
c, -NR
bR
c; wherein R
b and R
c are as defined above.
[0025] In some embodiments, in the definition of B-1, B-2, B-4, B-6 to B-8, B-10 to B-13,
B-17, the R
C is H, F, Cl, -OR
c, -SR
c, -NR
bR
c or R
c; wherein R
c is as defined above.
[0026] In some embodiments, in the definition of B-15, B-16, R
G is H.
[0027] In some embodiments, in the definition of B-15, B-16, R
F is H.
[0028] In some embodiments, in the definition of B-18 to B-20, V
3 is N or CH.
[0029] In some embodiments, in the definition of B-18 to B-20, V
2 is CH.
[0030] In some embodiments, in the definition of B-18 to B-20, W
1 is N or CH.
[0031] In some embodiments, in the definition of B-18 to B-20, W
2 is CR
F'; wherein R
F' is H or -NH
2.
[0032] In some embodiments, in the definition of B-18 to B-20, W
3 is CH.
[0033] In some embodiments, in the definition of B-18, W
4 is CH.
[0034] In some embodiments, B
1 is any of the following structures:

[0035] In some embodiments, B
1 is

[0036] In some embodiments, B
1 is

[0037] In some embodiments, B
1 is any of the following structures:

[0039] In some embodiments, B
1 is any of the following structures:

[0040] In some embodiments, B
1 is any of the following structures:

[0041] In some embodiments, B
1 is any of the following structures:

or

[0043] In some embodiments, B
2 is:

[0044] In some embodiments, B
2 is:

[0045] In some embodiments, B
2 is:

[0046] In some embodiments, when L is a connecting bond, then L
1 is CR
1R
2; R
1 and R
2 are defined as previously described.
[0047] In some embodiments, when L is CR
1R
2, then L
1 is a connecting bond; R
1 and R
2 are defined as previously described.
[0048] In some embodiments, when L' is a connecting bond, then L
1' is CR
11R
21; R
11 and R
21 are defined as previously described.
[0049] In some embodiments, when L' is CR
11R
21, then L
1' is a connecting bond; R
11 and R
21 are defined as previously described.
[0050] In some embodiments, L
2 is CH
2.
[0051] In some embodiments, L
2' is CH
2.
[0052] In some embodiments, X
1 is O.
[0053] In some embodiments, X
11 is O.
[0054] In some embodiments, X
2 is O.
[0055] In some embodiments, X
21 is O.
[0056] In some embodiments, R
a is C
1-4 alkyl or halo-C
1-4 alkyl.
[0057] In some embodiments, R
a is C
1-4 alkyl.
[0058] In some embodiments, R
1 and R
2 are each independently hydrogen, halogen, hydroxyl, or OR
a; R
a is defined as previously described.
[0059] In some embodiments, R
11 and R
21 are each independently hydrogen, halogen, hydroxyl, or OR
a; R
a is defined as previously described.
[0060] In some embodiments, R
1 is hydrogen.
[0061] In some embodiments, R
2 is hydrogen, fluorine, hydroxyl or methoxy.
[0062] In some embodiments, R
11 is hydrogen.
[0063] In some embodiments, R
21 is hydrogen, fluorine, hydroxyl or methoxy.
[0064] In some embodiments, R is hydrogen.
[0065] In some embodiments, R' is hydrogen.
[0066] In some embodiments, certain groups in the compound represented by Formula I, the
isomer, prodrug, stable isotope derivative or pharmaceutically acceptable salt thereof
can be as defined below, and undescribed groups can be as defined in any of the above
embodiments:
Z1 is O;
Z2 is O;
B1 is

B2 is

L and L1 are each independently a connecting bond or CR1R2;
L' and L1' are each independently a connecting bond or CR11R21; and, L, L1, L' and L1' are defined in the following combinations:
1) L is a connecting bond, L1 is CR1R2, L' is CR11R21, L1' is a connecting bond, or
2) L is CR1R2, L1 is a connecting bond, L' is a connecting bond, L1' is CR11R21;
L2 is CH2;
L2' is CH2;
X1 is O;
X11 is O;
X2 is O;
X21 is O;
X3 and X31 are each independently OH or SH;
R and R' are each independently hydrogen;
R1 and R2 are each independently hydrogen, halogen, hydroxyl or ORa;
R11 and R21 are each independently hydrogen, halogen, hydroxyl or ORa;
each Ra is independently C1-4 alkyl or halo-C1-4 alkyl.
[0067] In some embodiments, certain groups in the compound represented by Formula I, the
isomer, prodrug, stable isotope derivative or pharmaceutically acceptable salt thereof
can be as defined below, and undescribed groups can be as defined in any of the above
embodiments:
Z1 is O;
Z2 is O;
B1 is

B2 is

L and L1 are each independently a connecting bond or CR1R2;
L' and L1' are each independently a connecting bond or CR11R21; and, L, L1, L' and L1' are as defined in the following combinations:
1) L is a connecting bond, L1 is CR1R2, L' is CR11R21, L1' is a connecting bond, or
2) L is CR1R2, L1 is a connecting bond, L' is a connecting bond and L1' is CR11R21;
L2 is CH2;
L2' is CH2;
X1 is O;
X11 is O;
X2 is O;
X21 is O;
X3 and X31 are each independently OH or SH;
R and R' are each independently hydrogen;
R1 and R2 are each independently hydrogen, halogen, hydroxyl or ORa;
R11 and R21 are each independently hydrogen, halogen, hydroxyl or ORa;
each Ra is independently C1-4 alkyl or halo-C1-4 alkyl.
[0068] In some embodiments, certain groups in the compound represented by Formula I, the
isomer, prodrug, stable isotope derivative or pharmaceutically acceptable salt thereof
can be as defined below, and undescribed groups can be as defined in any of the above
embodiments:
Z1 is O;
Z2 is O;
B1 is

B2 is

L and L1 are each independently a connecting bond or CR1R2;
L' and L1' are each independently a connecting bond or CR11R21; and, L, L1, L' and L1' are as defined in the following combinations:
- 1) L is a connecting bond, L1 is CR1R2, L' is CR11R21, L1' is a connecting bond, or
- 2) L is CR1R2, L1 is a connecting bond, L' is a connecting bond, L1' is CR11R21;
L2 is CH2;
L2' is CH2;
X1 is O;
X11 is O;
X2 is O;
X21 is O;
X3 and X31 are each independently SH;
R and R' are each independently hydrogen;
R1 is hydrogen;
R2 is hydrogen, halogen, hydroxyl or ORa;
R11 is hydrogen;
R21 is hydrogen, halogen, hydroxyl or ORa;
each Ra is independently C1-4 alkyl.
[0069] In some embodiments, certain groups in the compound represented by Formula I, the
isomer, prodrug, stable isotope derivative or pharmaceutically acceptable salt thereof
can be as defined below, and undescribed groups can be as defined in any of the above
embodiments:
Z1 is O;
Z2 is O;
B1 is

B2 is

L and L1 are each independently a connecting bond or CR1R2;
L' and L1' are each independently a connecting bond or CR11R21,; and, L, L1, L' and L1' are as defined in the following combinations:
- 1) L is a connecting bond, L1 is CR1R2, L' is CR11R21, L1' is a connecting bond, or
- 2) L is CR1R2, L1 is a connecting bond, L' is a connecting bond and L1' is CR11R21;
L2 is CH2;
L2' is CH2;
X1 is O;
X11 is O;
X2 is O;
X21 is O;
X3 and X31 are SH;
R and R' are hydrogen;
R1 is hydrogen;
R2 is hydrogen, halogen, hydroxyl or ORa;
R11 is hydrogen;
R21 is hydrogen, halogen, hydroxyl or ORa;
each Ra is independently C1-4 alkyl.
[0070] In some embodiments, the compound of Formula I, the isomer, prodrug, stable isotope
derivative or pharmaceutically acceptable salt thereof, is the compound of Formula
II, III, IV or V, an isomer, prodrug, stable isotope derivative or pharmaceutically
acceptable salt thereof:

wherein, B
1, B
2, Z
1, Z
2, R, R', R
1, R
2, R
11, R
21, X
3, X
31, L
2 and L
2' are defined as described previously.
[0071] The combinations including any of the B
1, B
2, Z
1, Z
2, R, R', R
1, R
2, R
11, R
21, X
3, X
31, L2 and L
2' embodiments as described in Formula I are included in the scope of the Formula II,
III, IV or V in the present disclosure.
[0072] All embodiments of Formula II, III, IV or V described below are included in the scope
of the Formula II, III, IV or V in the present disclosure.
[0073] In some preferred embodiments of Formula II, III, IV or V, R
1 is H; R
2 is -OH, F, -N3, -SCF
3 or -OCH
3.
[0074] In some preferred embodiments of Formula II, III, IV or V, R
11 is H; R
21 is -OH, F, - N
3, -SCF
3 or -OCH
3.
[0075] In some preferred embodiments of Formula II, III, IV or V, R is -CH
2-, R
1 is -O-, R and R
1 are interconnected to form heterocycloalkyl.
[0076] In some preferred embodiments of Formula II, III, IV or V, R' is -CH
2-, R
11 is -O-, R' and R
11 are interconnected to form heterocycloalkyl.
[0077] In some preferred embodiments of Formula II, III, IV or V, Z
1 is O; Z2 is O.
[0078] In some preferred embodiments of Formula II, III, IV or V, L2 is -CH2-; L
2' is -CH2-.
[0079] In some embodiments, the compound of Formula I, the isomer thereof, prodrug, stable
isotope derivative or pharmaceutically acceptable salt thereof, is preferably the
compound of Formula VI or VII, an isomer, prodrug, stable isotope derivative or pharmaceutically
acceptable salt thereof:

wherein, B
1, B
2, R
2 and R
21 are defined as previously described.
[0080] The combinations including any of the B
1, B
2, R
2 and R
21 embodiments as described in Formula I are included in the scope of the Formula VI
or VII in the present disclosure.
[0081] All embodiments of Formula VI or VII described below are included in the scope of
the Formula VI or VII in the present disclosure.
[0082] In some preferred embodiments of Formula VI or VII, R
2 is -OH.
[0083] In some preferred embodiments of Formula VI or VII, R
2 is F.
[0084] In some preferred embodiments of Formula VI or VII, R
21 is -OH.
[0085] In some preferred embodiments of Formula VI or VII, R
21 is F.
[0086] In some preferred embodiments of Formula VI or VII, B
1 is

[0087] In some preferred embodiments of Formula VI or VII, B
2 is

[0088] In some preferred embodiments of Formula VI or VII, the stereo configuration is (Sp,
Sp), (Sp, Rp), (Rp, Rp), or (Rp, Sp).
[0089] In some preferred embodiments of Formula VI, R
2 is -OH; R
21 is -OH.
[0090] In some preferred embodiments of Formula VII, R
2 is -OH or -OCH3; R
21 is -OH or F.
[0092] In some embodiments, the compound of Formula I, the isomer, prodrug, stable isotope
derivative or pharmaceutically acceptable salt is any of the following structures:

[0096] The compound of formula (I), the pharmaceutically acceptable salt thereof can be
synthesized by a general chemical method.
[0097] In general, the preparation of the salt can be carried out by reacting the free base
or acid with an equivalent chemical equivalent or an excess of an acid (inorganic
or organic acid) or a base (inorganic or organic base) in a suitable solvent or solvent
composition.
[0098] The present disclosure also provides a pharmaceutical composition comprising a therapeutically
effective amount of an active component and a pharmaceutically acceptable excipient;
the active component comprises one or more of the cyclic di-nucleotide analogue (I),
the isomer thereof, prodrug, stable isotope derivative and pharmaceutically acceptable
salt thereof.
[0099] The active component in the pharmaceutical composition can also include other therapeutic
agents for viral infections or other infectious diseases (e.g., HIV, HBV, HCV infection,
etc.), autoimmune diseases (e.g., rheumatoid arthritis, lupus erythematosus, psoriasis,
etc.) or malignancies.
[0100] In the pharmaceutical composition, the pharmaceutically acceptable excipient can
include a pharmaceutically acceptable carrier, diluent, and/or excipient.
[0101] According to the purpose of the treatment, the pharmaceutical composition can be
formulated into various types of unit dosage forms, such as tablets, pills, powders,
liquids, suspensions, emulsions, granules, capsules, suppositories, and injections
(solutions and suspensions) and the like, and preferably liquids, suspensions, emulsions,
suppositories and injections (solutions and suspensions), etc.
[0102] In order to shape the pharmaceutical composition in the form of a tablet, any excipient
known and widely used in the art can be used. For example, carriers such as lactose,
white sugar, sodium chloride, glucose, urea, starch, calcium carbonate, kaolin, crystalline
cellulose, and silicic acid; binders such as water, ethanol, propanol, common syrup,
dextrose solution, starch solution, gelatin solution, carboxymethyl cellulose, shellac,
methyl cellulose and potassium phosphate, polyvinylpyrrolidone, etc.; disintegrating
agents, such as dry starch, sodium alginate, agar powder, kelp powder, sodium bicarbonate,
calcium carbonate, fatty acid esters of polyethylene dehydrated sorbitol, sodium dodecyl
sulfate, monoglyceryl stearate, starch and lactose; disintegration inhibitors such
as white sugar, glyceryl tristearate, coconut oil and hydrogenated oil; adsorption
promoters such as quaternary ammonium bases and sodium dodecyl sulfate; wetting agents
such as glycerin, starch, etc.; adsorbents such as starch, lactose, kaolin, bentonite
and colloidal silicic acid; and lubricating agents such as pure talc, stearate, boric
acid powder and polyethylene glycol. It is also possible to use a usual coating material
to formulate a sugar-coated tablet, a gelatin film tablet, a casing tablet, a film-coated
tablet, a two-layer film tablet, and a multilayer tablet.
[0103] In order to shape the pharmaceutical composition in the form of a pill, any excipient
known and widely used in the art may be used, for example, carriers such as lactose,
starch, coconut oil, hardened vegetable oil, kaolin and talc, etc.; binders such as
gum arabic powder, tragacanth powder, gelatin and ethanol, etc.; disintegrating agents
such as agar and kelp powder.
[0104] In order to shape the pharmaceutical composition in the form of a suppository, any
excipient known and widely used in the art can be used, for example, polyethylene
glycol, coconut oil, higher alcohols, esters of higher alcohols, gelatin and semi-synthetic
glycerides, etc.
[0105] For the preparation of a pharmaceutical composition in the form of an injection,
the solution or suspension may be sterilized (preferably by adding an appropriate
amount of sodium chloride, glucose or glycerin, etc.) to prepare an injection which
is isotonic with blood. Any of the commonly used carriers in the art can also be used.
For example, water, ethanol, propylene glycol, ethoxylated isostearyl alcohol, polyoxylated
isostearyl alcohol, and fatty acid esters of polyethylene dehydrated sorbitol. In
addition, usual solubilizers, buffers, analgesics can be added.
[0106] In the present disclosure, the content of the composition in the pharmaceutical composition
is not particularly limited and can be selected in a wide range, usually 5-95% by
mass, preferably 30-80% by mass.
[0107] In the present disclosure, the administration method of the pharmaceutical composition
is not particularly limited. Formulations of various dosage forms can be selected
depending on the age, sex and other conditions and symptoms of the patient. For example,
tablets, pills, solutions, suspensions, emulsions, granules or capsules are administered
orally; injections can be administered alone or in combination with solutions for
injection (e.g., glucose solution and amino acid solution); suppositories are given
to the rectum.
[0108] The present disclosure also provides a use of the cyclic di-nucleotide analogue (I),
the isomer thereof, prodrug, stable isotope derivative or pharmaceutically acceptable
salt, or the pharmaceutical composition in the preparation of interferon gene stimulating
factor STING modulators. The interferon gene stimulating factor STING modulator is
preferably interferon gene stimulating factor STING agonist. The STING agonist refers
to the compound of formula (I), the isomer, prodrug, stable isotope derivative or
pharmaceutically acceptable salt thereof, or the pharmaceutical composition capable
of activating STING signaling.
[0109] The present disclosure also provides a use of the cyclic di-nucleotide analogue (I),
the isomer thereof, prodrug, stable isotope derivative or pharmaceutically acceptable
salt, or the pharmaceutical composition in the preparation of a medicament for modulation
of the proliferation of T cells or other immune cells.
[0110] The present disclosure also provide a use of the cyclic di-nucleotide analogue (I),
the isomer thereof, prodrug, stable isotope derivative or pharmaceutically acceptable
salt, or the pharmaceutical composition in the preparation of a vaccine adjuvant.
[0111] The present disclosure also provide a use of the cyclic di-nucleotide analogue (I),
the isomer thereof, prodrug, stable isotope derivative or pharmaceutically acceptable
salt, or the pharmaceutical composition in the preparation of a medicament for the
treatment and/or alleviation of a STING-mediated neoplastic disease and non-neoplastic
disease. The STING-mediated diseases refer to diseases caused by immunosuppression
or hyperactivation due to STING signaling. The types of related diseases include,
but are not limited to: viral infections or other infectious diseases, autoimmune
diseases, and malignancies.
[0112] The present disclosure preferably provides a use of the cyclic di-nucleotide analogue
(I), the isomer thereof, prodrug, stable isotope derivative or pharmaceutically acceptable
salt, or the pharmaceutical composition in the preparation of a medicament for the
treatment and/or alleviation of malignancies caused by immunosuppression.
[0113] The present disclosure further provides a method of treating viral infections or
other infectious diseases, malignancies, autoimmune diseases with the cyclic di-nucleotide
analogue (I), the isomer thereof, prodrug, stable isotope derivative or pharmaceutically
acceptable salt, or the pharmaceutical composition, comprising: administering to a
mammal a required dose of the cyclic di-nucleotide analogue (I), the isomer thereof,
prodrug, stable isotope derivative or pharmaceutically acceptable salt, or the pharmaceutical
composition.
[0114] The mammal is preferably human.
[0115] The present disclosure preferably provides the compound represented by formula (I),
the isomer thereof, prodrug, stable isotope derivative or pharmaceutically acceptable
salt, or the pharmaceutical composition in the preparation of a medicament for the
treatment and/or alleviation of a STING-mediated disease; the STING-mediated disease
is those caused by STING mediated immunosuppression, the diseases can include: viral
infections or other infectious diseases (e.g., HIV, HBV, HCV infection, etc.), autoimmune
diseases (e.g., rheumatoid arthritis, lupus erythematosus, psoriasis, etc.), or malignancies.
[0116] The present disclosure further provides a use of the compound represented by formula
(I), the isomer thereof, prodrug, stable isotope derivative or pharmaceutically acceptable
salt, or the pharmaceutical composition in the preparation of a medicament for the
treatment and/or alleviation of malignancies.
[0117] The present disclosure further provides a use of the compound represented by formula
(I), the isomer thereof, prodrug, stable isotope derivative or pharmaceutically acceptable
salt, or the pharmaceutical composition in the preparation of a medicament for the
treatment and/or alleviation of viral or other infections.
[0118] The present disclosure further provides a use of the compound represented by formula
(I), the isomer thereof, prodrug, stable isotope derivative or pharmaceutically acceptable
salt, or the pharmaceutical composition in the preparation of a medicament for the
treatment and/or alleviation of autoimmune diseases.
[0119] The present disclosure further provides the cyclic di-nucleotide analogue (I), the
isomer thereof, prodrug, stable isotope derivative or pharmaceutically acceptable
salt, or the pharmaceutical composition in combination with one or more other kinds
of therapeutic agents and/or therapeutic methods for use in the treatment, alleviation
and/or prevention of STING-mediated diseases. The STING-mediated diseases are those
caused by STING-mediated immunosuppression, and the diseases can include: viral or
other infections (e.g., HIV, HBV, HCV infections, etc.), autoimmune diseases (e.g.,
rheumatoid arthritis, lupus erythematosus, psoriasis, etc.), or cancer.
[0120] The present disclosure preferably provides the cyclic di-nucleotide analogue (I),
the isomer thereof, prodrug, stable isotope derivative or pharmaceutically acceptable
salt, or the pharmaceutical composition in combination with one or more other kinds
of therapeutic agents and/or therapeutic methods for use in the treatment and/or alleviation
of cancer.
[0121] The present disclosure preferably provides the cyclic di-nucleotide analogue (I),
the isomer thereof, prodrug, stable isotope derivative or pharmaceutically acceptable
salt, or the pharmaceutical composition in combination with one or more other kinds
of therapeutic agents and/or therapeutic methods for use in the treatment and/or alleviation
of STING-mediated cancer.
[0122] In the present disclosure, the other kinds of therapeutic agents (e.g., other kinds
of therapeutic agents for the treatment of cancer) can be made into a therapeutic
dosage form with the cyclic di-nucleotide analogue (I) for a single dosage form, or
separate therapeutic dosage forms for sequential administration.
[0123] The present disclosure further provides a combination formulation comprising the
compound of formula (I), the isomer thereof, prodrug, stable isotope derivative or
pharmaceutically acceptable salt, or the pharmaceutical composition and other kinds
of therapeutic agents and/or therapeutic methods for the treatment of cancer.
[0124] In the present disclosure, the other kinds of therapeutic agents for the treatment
of cancer can include, but are not limited to one or more of: microtubule protein
inhibitors, alkylating agents, topozyme I/II inhibitors, platinum compounds, antimetabolites,
hormones and hormone analogs, signal transduction pathway inhibitors, angiogenesis
inhibitors, targeted therapeutic agents (e.g., specific kinase inhibitors), immunotherapeutic
agents, pro-apoptotic agents, and cell cycle signaling pathway inhibitors.
[0125] In the present disclosure, the other kinds of therapeutic methods for the treatment
of cancer can include, but are not limited to, one or more of: tumor immunotherapy
and radiotherapy.
[0126] In the present disclosure, the other kinds of therapeutic agents for the treatment
of cancer are preferably immunotherapeutic agents.
[0127] In the present disclosure, the microtubulin inhibitor may be selected from, but is
not limited to, one or more of: the vincristine family (e.g., vinblastine, vincristine,
vinorelbine, vindesine sulfate), the taxane family (docetaxel, paclitaxel), and eribulin
mesylate.
[0128] In the present disclosure, the alkylating agent may be selected from, but is not
limited to: nitrogen mustard, N-oxo-nitrogen mustard hydrochloride, cyclobutoic nitrogen
mustard, uracil mustard, cyclophosphamide, ifosfamide, thiotepa, carboquone, trisethyleneiminoquinone,
improsulfan tosylate, mannosesufan, treosulfan, busulfan, nimustine hydrochloride,
dibromomannitol, melphalan, dacarbazine, ranimustine, carmustine, lomustine, streptozotocin,
temozolomide, procarbazine, ethyleneimine derivatives, methanesulfonates, nitrosoureas,
triazenes.
[0129] In the present disclosure, the topozyme I/II inhibitors may be selected from, but
not limited to, one or more of: doxorubicin, daunorubicin, epirubicin, idarubicin,
irinotecan, topotecan, rubitecan, belotecan, etoposide, teniposide, adriamycin, and
dexrazoxane, camptothecin.
[0130] In the present disclosure, the platinum compound may be selected from, but not limited
to, one or more of: cisplatin, carboplatin, oxaliplatin, and nedaplatin.
[0131] In the present disclosure, the antimetabolites may be selected from, but not limited
to, one or more of: folate antagonists, pyrimidine analogs, purine analogs, adenosine
deaminase inhibitors, such as: methotrexate, 5-fluorouracil, floxuridine, cytarabine,
6-mercaptopurine, 6-thioguanine, fludarabine phosphate, pentostatin, and gemcitabine.
[0132] In the present disclosure, the immunotherapeutic agent may be selected from, but
not limited to one or more of: immunomodulators, tumor microenvironment modulators,
and anti-angiogenic factors. The immunomodulators may include, but are not limited
to: 1) protein antagonists (e.g., immune checkpoint inhibitors) that inhibit T-cell
activity: one or more of CTLA4 (e.g., one or more of ipilimumab, tremelimumab, abatacept,
belatacept, BMS-986249, BMS-986218, AGEN-1884 and KN-046), PD-1 (e.g., one or more
of camrelizumab, toripalimab, sintilimab, cemiplimab, pembrolizumab, nivolumab, tislelizumab,
spartalizumab,, dostarlimab, genolimzumab, cetrelimab, HLX-10, BCD-100, AK-105, MEDI-0680,
CS-1003, BAT-1306, HX-008, sasanlimab, AGEN-2034, BI-754091, GLS-010, MGA-012, AK-104
and AK-103), PD-L1 (e.g., one or more of durvalumab, avelumab, atezolizumab, envafolimab,
cosibelimab, CS1001, SHR-1316, lazertinib, bintrafusp alfa, TQB-2450, CA-170, CX-072,
BGB-A333, BMS-936559, GEN-1046, KL-A167 and 10-103), LAG3, and TIM3; 2) protein agonists
that stimulate T-cell activity: one or more of GITR, OX40, OX40L, 4-1BB (CD137), CD27
and CD40; 3) one or more of TLR2 agonists, TLR4 agonists, TLR5 agonists, TLR7 agonists,
TLR8 agonists and TLR9 agonists; (4) IDO inhibitors, CD73 inhibitors.
[0133] In the present disclosure, the signal transduction pathway inhibitors (STI) may be
selected from, but not limited to, one or more of: BCR/ABL kinase inhibitors, epidermal
growth factor receptor inhibitors, her-2/neu receptor inhibitors, AKT family kinase
inhibitors, PI3K signaling pathway inhibitors, and cell cycle checkpoint inhibitors.
[0134] In the present disclosure, the angiogenesis inhibitors may be selected from, but
not limited to, one or more of: VEGF/VEGFR signaling pathway inhibitors, Src family
kinase inhibitors, Src signaling pathway inhibitors, and c-Fes kinase inhibitors.
[0135] In the present disclosure, the targeted therapeutic agents may be selected from,
but not limited to: one or more of erlotinib, imatinib, apatinib, nilotinib, crizotinib,
dasatinib, pazopanib, regorafenib, ruxolitinib, sorafenib, sunitinib, vandetanib,
vemurafenib, bosutinib, gefitinib, afatinib, axitinib, dabrafenib, dacomitinib, nintedanib,
levatinib, masitinib, midostaurin,neratinib, ponatinib, radotinib, trametinib, brivanib
alaninate, cediranib, cabozantinib malate, ibrutinib,icotinib, lapatinib, cobimetinib,
idelalisib, ponatinib, alisertib, dinaciclib, linsitinib, orantinib, rigosertib, tipifarnib,
tivozanib, pimasertib, buparlisib, and fedratinib.
[0136] In the present disclosure, the tumor immunotherapy may be selected from, but not
limited to, one or more of: antitumor vaccines (e.g., synthetic peptides, DNA vaccines,
and recombinant viruses), oncolytic viruses, cytokine therapies (e.g., IL2 and GM-CSF),
and chimeric antigen receptor T-cell therapies (CAR-T).
[0137] In the present disclosure, the viral and other infections may include: the infections
caused by viruses such as influenza viruses, hepatitis B virus (HBV), hepatitis C
virus (HCV), human papillomavirus (HPV), cytomegalovirus (CMV), Epstein-Barr virus
(EBV), poliovirus, varicella-zoster virus, coxsackievirus, or human immunodeficiency
virus (HIV).
[0138] In the present disclosure, the malignant tumors include metastatic and non-metastatic
cancers, also include familial hereditary and sporadic cancers, and may also include
solid and non-solid tumors.
[0139] In the present disclosure, specific examples of the solid tumors may include, but
not limited to: eye, bone, lung, stomach, pancreas, breast, prostate, brain (including
glioblastoma and medulloblastoma), ovary (including those stromal cells, germ cells
and mesenchymal cells arising from epithelial cells), bladder, testis, spinal cord,
kidney (including adenocarcinoma, nephroblastoma), mouth, lip, throat, oral cavity
(including squamous cell carcinoma), nasal cavity, small intestine, colon, rectum,
parathyroid gland, gallbladder, bile duct, cervix, heart, subpharyngeal gland, bronchus,
liver, ureter, vagina, anus, laryngeal gland, thyroid gland (including thyroid cancer
and medullary carcinoma), esophagus, nasopharyngeal gland pituitary, salivary gland,
adrenal gland, intraepithelial neoplasia of head and neck (including Bowen's disease
and Paget's disease), sarcoma (including smooth muscle sarcoma, rhabdomyosarcoma,
liposarcoma, fibrosarcoma, osteosarcoma), skin (including melanoma, Kaposi's sarcoma,
basocellular carcinoma and squamous cell carcinoma) and other related tumors.
[0140] In the present disclosure, the solid tumor is preferably one or more of eye cancer,
bone cancer, lung cancer, stomach cancer, pancreatic cancer, breast cancer, prostate
cancer, brain cancer (including but not limited to glioblastoma, adult neural tube
cell tumor), ovarian cancer, bladder cancer, cervical cancer, testicular cancer, kidney
cancer (including but not limited to adenocarcinoma, nephroblastoma), oral cancer
(including squamous cell carcinoma), tongue cancer, laryngeal cancer, nasopharyngeal
cancer, head and neck cancer, colon cancer, small intestine cancer, rectal cancer,
parathyroid cancer, thyroid cancer, esophageal cancer, gallbladder cancer, bile duct
cancer, cervical cancer, liver cancer, lung cancer (including but not limited to small
cell lung cancer, non-small cell lung cancer), chorionic epithelioma, osteosarcoma,
ewing tumor, soft tissue sarcoma and skin cancer.
[0141] In the present disclosure, specific examples of the non-solid tumors (including hematological
tumors) may include, but not limited to: lymphoid leukemia (including acute lymphoblastic
leukemia, lymphoma, myeloma, chronic lymphocytic leukemia, Hodgkin's lymphoma, non-Hodgkin's
lymphoma, T-cell chronic lymphatic leukemia, B-cell chronic lymphatic leukemia), myeloid-associated
leukemia (including acute myeloid leukemia, chronic myeloid leukemia) and AIDs-associated
leukemia.
[0142] In the present disclosure, the autoimmune diseases may include, but not limited to:
one or more of rheumatoid arthritis, systemic lupus erythematosus, mixed connective
tissue disease (MCTD), systemic scleroderma (including: CREST syndrome), dermatomyositis,
nodular vasculitis, renal diseases (including: pulmonary hemorrhagic nephritis syndrome,
acute glomerulonephritis, primary membranoproliferative glomerulonephritis, etc.),
endocrine-related diseases (including: type I diabetes, gonadal insufficiency, pernicious
anemia, hyperthyroidism, etc.), liver disease (including: primary biliary cirrhosis,
autoimmune cholangitis, autoimmune hepatitis, primary sclerosing cholangitis, etc.)
and autoimmune reactions caused by infections (e.g., AIDS, malaria, etc.).
[0143] Unless otherwise stated, the following terms appearing in the specification and claims
of the disclosure have the following meanings:
[0144] The term "alkyl" refers to a saturated straight or branched-chain hydrocarbon group
comprising 1 to20 carbon atoms, preferably 1 to 10 carbon atoms, more preferably 1
to8, 1 to6, 1 to4, 1 to3 carbon atoms, representative examples of alkyl groups include,
but are not limited to: methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl,
tert-butyl, isobutyl,
n-pentyl,
n-hexyl,
n-heptyl, octyl, nonyl, decyl, 1,1-dimethylpropyl, 1,2-dimethylpropyl, 2,2-dimethylpropyl,
1-ethylpropyl, 2-methylbutyl, 3-methylbutyl, 1-ethyl-2-methylpropyl, 1,1,2-trimethylpropyl,
1,1-dimethylbutyl, 1,2-dimethylbutyl, 2,2-dimethylbutyl, 1,3-dimethylbutyl, 2,3-dimethylbutyl,
2-ethylbutyl, 2-methylpentyl, 3-methylpentyl, 4-methylpentyl, 4,4-dimethylpentyl,
2-methylhexyl, 3-methylhexyl, 4-methylhexyl, 5-methylhexyl, 2,3-dimethylpentyl, 2,4-dimethylpentyl,
2,2-dimethylpentyl, 3,3-dimethylpentyl, 2-ethylpentyl, 3-ethylpentyl, 2,2,4-trimethylpentyl,
undecyl, dodecyl, and their various isomers, etc.
[0145] The term "cycloalkyl" refers to a saturated or partially unsaturated (containing
1 or 2 double bonds) monocyclic or polycyclic group containing 3 to20 carbon atoms.
The term "monocyclic cycloalkyl" is preferably a 3 to 10 membered monocyclic alkyl,
more preferably a 3 to8 membered monocyclic alkyl, such as: cyclopropyl, cyclobutyl,
cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, cyclodecyl, cyclohexenyl. The term
"polycyclic cycloalkyl" includes "bridged cycloalkyl", "fused cycloalkyl" and "spiro
cycloalkyl". Monocyclic cycloalkyl or polycyclic cycloalkyl can be linked to the parent
molecule by any carbon atom on the ring.
[0146] The term "heterocycloalkyl" refers to a saturated or partially unsaturated (containing
1 or 2 double bonds) non-aromatic cyclic group consisting of carbon atom(s) and heteroatom(s)
selected from nitrogen, oxygen or sulfur, which may be monocyclic or polycyclic, in
the present disclosure the number of heteroatom(s) in the heterocycloalkyl group is
preferably 1, 2, 3 or 4, the nitrogen, carbon or sulfur atom in the heterocycloalkyl
group may optionally be oxidized. The nitrogen atom may optionally be further substituted
with other groups to form tertiary amines or quaternary ammonium salts. The "monocyclic
heterocycloalkyl" is preferably a 3 to 10 membered monocyclic heterocycloalkyl, more
preferably a 3 to 8 membered monocyclic heterocycloalkyl. For example: aziridinyl,
tetrahydrofuran-2-yl, morpholin-4-yl, thiomorpholin-4-yl, thiomorpholine-S-oxide-4-yl,
piperidin-1-yl,
N-alkylpiperidin-4-yl, pyrrolidin-1-yl,
N-alkylpyrrolidin-2-yl, piperazin-1-yl, 4-alkylpiperazin-1-yl, etc. "Polycyclic heterocycloalkyl"
includes "fused heterocycloalkyl", "spiro heterocycloalkyl" and "bridged heterocycloalkyl".
Monocyclic heterocycloalkyl and polycyclic heterocycloalkyl can be linked to the parent
molecule by any ring atom on the ring. The above ring atoms refer specifically to
the carbon and/or nitrogen atoms that constitute the ring skeleton.
[0147] The term "cycloalkyl alkyl" refers to a cycloalkyl group connected to the parent
nucleus structure through an alkyl group. Thus, the term "cycloalkyl alkyl" encompasses
the above definitions of alkyl and cycloalkyl.
[0148] The term "heterocycloalkyl alkyl" refers to a heterocycloalkyl group connected to
the parent nucleus structure through an alkyl group. Thus, the term "heterocycloalkyl"
encompasses the above definitions of alkyl and heterocycloalkyl.
[0149] The term "alkoxy" refers to a cyclic or non-cyclic alkyl group having indicated carbon
atoms attached through an oxygen bridge, and includes alkyloxy, cycloalkyloxy and
heterocycloalkyloxy. Thus, "alkoxy" includes the above definitions of alkyl, heterocycloalkyl,
and cycloalkyl.
[0150] The term "alkenyl" refers to a straight, branched or cyclic non-aromatic hydrocarbon
group containing at least 1 carbon-carbon double bond. There may be 1 to 3 carbon-carbon
double bonds present, preferably 1 carbon-carbon double bond present. The term "C2-4
alkenyl" refers to an alkenyl group having 2 to 4 carbon atoms, and the term "C2-6
alkenyl" refers to an alkenyl group having 2 to 6 carbon atoms, including vinyl, propylenyl,
butenyl, 2-methylbutenyl, and cyclohexenyl. The alkenyl groups can be substituted.
[0151] The term "alkynyl" refers to a straight, branched or cyclic hydrocarbon group containing
at least 1 carbon-carbon triple bond. There may be 1 to 3 carbon-carbon triple bonds
present, preferably 1 carbon-carbon triple bond present. The term "C
2-6 alkynyl" refers to an alkynyl group having 2 to 6 carbon atoms, including ethynyl,
propinyl, butynyl and 3-methylbutynyl.
[0152] The term "aryl" refers to any stable 6 to 10 monocyclic or bicyclic aromatic group,
such as: phenyl, naphthyl, tetrahydronaphthyl, 2,3-dihydroindenyl, or biphenyl.
[0153] The term "heteroaryl" refers to an aromatic cyclic group formed by replacing at least
1 carbon atom on the ring by a heteroatom selected from nitrogen, oxygen or sulfur,
which may be a 5 to 7 membered monocyclic structure or a 7 to 12 membered bicyclic
structure, preferably 5 to 6 membered heteroaryl. In the present disclosure, the number
of the heteroatom is preferably 1, 2 or 3, and includes: pyridyl, pyrimidinyl, pyridazin-3(2
H)-one group, furanyl, thienyl, thiazolyl, pyrrolyl, imidazolyl, pyrazolyl, oxazolyl,
isoxazolyl, 1,2,5-oxadiazolyl, 1,2,4-oxadiazolyl, 1,2,4-triazolyl, 1,2,3-triazolyl,
tetrazolyl, indazolyl, isoindazolyl, indolyl, isoindolyl, benzofuranyl, benzothienyl,
benzo[
d][1,3]dioxolyl, benzothiazolyl, benzoxazolyl, quinolinyl, isoquinolinyl, quinazolinyl,
etc.
[0154] The term "arylalkyl" refers to an aryl group connected to the parent nucleus structure
through an alkyl group. Thus, the term "arylalkyl" encompasses the above definitions
of the alkyl and aryl.
[0155] The term "heteroaryl-alkyl" refers to a heterocycloalkyl group connected to the parent
nucleus structure through an alkyl group. Thus, the term "heteroarylalkyl" encompasses
the above definitions of the alkyl and heteroaryl.
[0156] The term "halogen" indicates fluorine, chlorine, bromine or iodine.
[0157] The term "halo-alkyl" refers to an alkyl group arbitrarily substituted with halogen.
Thus, "halo-alkyl" includes the definitions of the halogen and alkyl above.
[0158] The term "halo-alkoxy" refers to an alkoxy group arbitrarily substituted with halogen.
Thus, the term "halo- alkoxy" encompasses the above definitions of the halogen and
alkoxy.
[0159] The term "amino" refers to -NH
2 and the term "alkylamino" refers to that at least one hydrogen atom on the amino
group is the substituted by an alkyl group, including but not limited to: -NHCH
3, -N(CH
3)
2, -NHCH
2CH
3, -N(CH
2CH
3)
2.
[0160] The term "nitro" refers to -NO
2.
[0161] The term "cyano" refers to -CN.
[0162] The term "azido" refers to -N3.
[0163] The term "room temperature" in the present disclosure refers to 15-30 °C.
[0164] The isotope-substituted derivative includes an isotope-substituted derivative that
any hydrogen atom of the compound of formula (I) is replaced by 1 to 5 deuterium atoms,
or any carbon atom of the compound of formula (I) is replaced by 1-3 C
14 atom, or any oxygen atom of the compound of formula I is replaced by 1 to 3 O
18 atom.
[0165] The term "prodrug" refers to a compound capable of converting to its original active
compound after metabolism
in vivo. Representatively, a prodrug is an inactive substance or less active than the active
parent compound, but may provide convenient handling, administration, or improved
metabolic properties.
[0166] The "pharmaceutically acceptable salts" described in the present disclosure are discussed
in
Berge, et al., "pharmaceutically acceptable salts", J. Pharm. Sci., 66, 1-19 (1977), and it is apparent to pharmaceutical chemists that the salts are substantially
non-toxic and provide the desired pharmacokinetic properties, palatability, absorption,
distribution, metabolism or excretion, etc. The compounds described herein may have
acidic groups, basic groups, or amphoteric groups, and typical pharmaceutically acceptable
salts include those obtained by reaction of compounds of the present disclosure with
acids, such as: hydrochloride, hydrobromide, sulfate, pyrosulfate, bisulfate, sulfite,
bisulfite, phosphate, monohydrogen phosphate, dihydrogen phosphate, metaphosphate,
pyrophosphate, nitrate, acetate, propionate, decanoate, octanoate, formate, acrylate,
isobutyrate, caproate, heptanoate, oxalate, malonate, succinate, octanedioate, benzoate,
methylbenzoate, phthalate, maleate, methanesulfonate,
p-toluenesulfonate, (
D,L)-tartaric acid, citric acid, maleic acid, (
D,L)-malic acid, fumaric acid, succinate, lactate, trifluoromethanesulfonate, naphthalene-1-sulfonate,
mandelate, pyruvate, stearate, ascorbate, salicylate. When the compounds of the disclosure
contain acidic groups, pharmaceutically acceptable salts thereof may also include:
alkali metal salts, such as lithium, sodium or potassium salts; alkaline earth metal
salts, such as zinc, calcium or magnesium salts; organic alkali salts, such as salts
formed with ammonia, alkylamines (including but not limited to: methylamine, triethylamine),
hydroxyalkylamines, amino acids (including but not limited to: lysine, arginine),
N-methylglucosamine, etc.
[0167] The term "isomer" in the present disclosure means that the compound of formula (I)
of the present disclosure may have asymmetric centers and racemates, racemic mixtures
and individual diastereoisomers, all of which are included in the present disclosure,
including stereoisomers and geometric isomers. In the present disclosure, the individual
stereoisomers (enantiomers and diastereoisomers), as well as mixtures thereof, are
included within the scope of the present disclosure when the compound of formula (I)
or its salt can be present in stereoisomeric form (e.g., it contains one or more asymmetric
carbon atoms and/or phosphorus atoms). The present disclosure also includes the individual
isomers of the compounds represented by formula (I) or salts, as well as mixtures
of isomers with one or more of the chiral centers reversed. The scope of the present
disclosure includes mixtures of stereoisomers, and purified enantiomeric or enantiomeric/diastereoisomeric
enriched mixtures. The present disclosure includes stereoisomeric mixtures formed
by all enantiomers and diastereoisomers in all possible different combinations. The
present disclosure includes all combinations and subsets of stereoisomers of all specific
groups as defined above. The compound of formula (I) in the present disclosure contains
chiral P-atoms with Rp or Sp conformation, so that compounds with individual stereo-configuration
of (Sp, Sp), (Sp, Rp), (Rp, Rp) or (Rp, Sp) and any mixture thereof are included in
the scope of the present disclosure.
[0168] The above preferred conditions of the present disclosure may be arbitrarily combined
without departing from the general knowledge in the art to obtain the preferred embodiments
of the present disclosure.
[0169] The reagents and raw materials used in the present disclosure are commercially available.
Brief description of the drawings
[0170] FIG.1 is the tumor volume change curves of compounds 6-p3 (1 mg/kg, 2 mg/kg, i.t.)
and Ref.1 (2 mg/kg, i.t.) in the colon cancer CT26 subcutaneously implanted tumor
in mouse.
Detailed description of the embodiment
[0171] The following Embodiments serve to illustrate the present disclosure, but the Embodiments
should not be considered as limiting the scope of the disclosure. Some of the experimental
methods of the following Embodiments that are not indicated the specific conditions,
can in according with the commonly used reaction conditions and procedures, or in
accordance with the product specifications.
[0172] All the structures of the compounds in the present disclosure were confirmed by Nuclear
Magnetic Resonance (
1H NMR) and/or Mass Spectra (MS).
[0173] 1H NMR chemical shifts (δ) were recorded in ppm (10
-6). NMR Spectra were recorded on Bruker AVANCE-400 spectrometer. The proper solvents
were Chloroform-d (CDCl
3), Methanol-d4 (CD
3OD), and Dimethyl sulfoxide-d
6 (DMSO-
d6), tetramethylsilane as internal standard (TMS).
[0174] The analytical low-resolution mass spectra (LCMS) were recorded on Agilent 1200 HPLC/6120
using an XBridge C18, 3.0×50 mm, 3µm, column temperature: 35°C; or recorded on ThermoUltiMate
3000HPLC/MSQPLUS using an XBridge C18, 3.0×50 mm, 3.5µm, column temperature: 30°C.
The gradient elution method 1 of Agilent: 95-5% solvent A
1 and 5-95% solvent B
1 (0-2.0min), and then 95% solvent B
1 and 5% solvent A
1 (for 1.1min). Percentage as used herein is volume percentage of the volume of a solvent
in the total solvent volume. Solvent A
1: 0.01% aqueous solution of trifluoroacetic acid (TFA); Solvent B
1: 0.01% trifluoroacetic acid acetonitrile solution. Percentage is the volume of a
solvent in the total solvent volume. The gradient elution method 2 of Thermo: 95-5%
solvent A
2 and 5-95% solvent B
2 (0-2min), and then 95% solvent B
2 and 5% solvent A
2 (for 1.8min), Percentage is the volume of a solvent in the total solvent volume.
Solvent A
2: 10 mM aqueous solution of ammonium bicarbonate; Solvent B
2: acetonitrile.
[0175] All the compounds in the present disclosure were separated by preparative high-performance
liquid chromatography or flash column chromatography.
[0176] Preparative high-performance liquid chromatography purification (
prep-HPLC) was performed on Shimadzu LC-20 HPLC, chromatographic column: waters xbridge
Pre C18, 10um, 19mmx250mm. Separation method 1 (acidic condition): mobile phase A:
0.05% aqueous solution of trifluoroacetic acid, mobile phase B: acetonitrile; elution
B was 40%, elution time: 20min. Separation method 2 (alkali condition): mobile phase
A: 10 mmol/L aqueous solution of ammonium bicarbonate, mobile phase B: acetonitrile;
the gradient elution B was from 10% to 80%, elution time: 30min. Separation method
3 (alkali condition): mobile phase A: 10 mmol/L aqueous solution of ammonium bicarbonate,
mobile phase B: acetonitrile; the gradient elution B was from 0% to 15%, elution time:
30min. Separation method 4 (alkali condition): mobile phase A: 10 mmol/L aqueous solution
of ammonium bicarbonate, mobile phase B: acetonitrile; the gradient elution B was
from 0% to 4%, elution time: 10min; the gradient elution B was from 4% to 8%, elution
time: 15min. Separation method 5 (alkali condition): mobile phase A: 10 mmol/L aqueous
solution of ammonium bicarbonate, mobile phase B: acetonitrile; the gradient elution
B was from 0% to 5%, elution time: 10min; the gradient elution B was from 5% to 10%,
elution time: 15min. Separation method 6 (alkali condition): mobile phase A: 10 mmol/L
aqueous solution of ammonium bicarbonate, mobile phase B: acetonitrile; the gradient
elution B was from 10% to 30%, elution time: 5min; the gradient elution B was from
30% to 75%, elution time: 20min. Separation method 7 (alkali condition): mobile phase
A: 10 mmol/L aqueous solution of ammonium bicarbonate, mobile phase B: acetonitrile;
the gradient elution B was from 0% to 10%, elution time: 7min; the gradient elution
B was from 10% to 40%, elution time: 18min. Detection wavelength: 214nm&254nm; the
flow rate: 15.0 mL/min.
[0177] Flash column chromatography (flash system/Cheetah™) was performed on Agela Technologies
MP200. Normal-phase chromatography column was Flash columm Silica-CS (25g, 40g, 80g,
120g or 330g), Agela Technologies, Tianjing. Ethyl acetate/ petroleum ether or dichloromethane/methanol
was chosen as elution system. Reversed-phase chromatography column was C18 columm
(12g, 20g or 40g), Santai Technologies, Changzhou. Acetonitrile/ aqueous solution
of ammonium bicarbonate (10mmol/L) were chosen as elution system.
[0178] All the compounds in the present disclosure were analyzed by high- performance liquid
chromatography. High- performance liquid chromatography (HPLC) was performed on Waters
e2695, 2498 UV/VIS Detector, chromatography column: Waters Xselect CHS C18 (4.6
∗150mm) 5µm, mobile phase A: acetonitrile, mobile phase B: acetic acid triethylamine
buffer solution which was adjusted pH to 5.0 with acetic acid. Gradient elution of
mobile phase B from 95% to 15%, elution time: 30min. Detection wavelength: 214nm&254nm;
column temperature: 35°C.
Embodiment 1: Synthesis of intermediate 1-8
[0179]

[0180] Step 1: To a suspension of adenosine (50g, 187mmol) in acetic acid/sodium acetate
buffer solution (pH=4.0, 0.5M, 1L) was added liquid bromine (60g, 374mmol), keep the
system temperature below 10°C. After addition, the reaction system was stirred at
room temperature for 48h. The saturated aqueous solution of sodium bisulfate was added
into the reaction solution to remove the excess bromine, and then adjusted pH to neutral
with an aqueous solution of sodium hydroxide (1M), the reaction solution was stirred
for 2h under ice-water bath. The precipitate was formed and collected by filtration,
dried under vacuum to afford intermediate 1-1 (29g). m/z: [M+H]+346.0/348.0.
[0181] Step 2: To a suspension of intermediate 1-1 (10g, 28.9mmol) in methanol (100 mL)
was added sodium methanolate (9.36g, 173mmol), the reaction system was stirred at
reflux for 5h, methanol was concentrated under reduced pressure. The residue was dissolved
in a mixed solvent of methanol/ dichloromethane (1/10). The solution was filtered
through a Büchner funnel which was covered with a layer of silica gel. The filtrate
was concentrated under reduced pressure to afford intermediate 1-2 (3.8g). m/z: [M+H]+
298.2.
[0182] Step 3: To a solution of intermediate 1-2 (10g, 336mmol) in pyridine (40mL) was added
chlorotrimethylsilane (16mL, 121mmol) under nitrogen at 0°C. The reaction system was
stirred at room temperature for 2h, benzoyl chloride (9.4mL, 80.7mmol) was slowly
added to the above reaction system. The resulting solution was stirred at room temperature
for overnight, and then ammonium hydroxide solution (25-28%) was added thereto and
stirred for 30min. The solvent was concentrate under reduced pressure. The residue
was purified by Flash column chromatography (0-10% methanol/ dichloromethane) to afford
intermediate 1-3 (7.3g) as a white solid. m/z: [M+H]+ 402.2.
[0183] Step 4: To a solution of intermediate 1-3 (7g, 17.4mmol) in anhydrous pyridine (40mL)
was added 4,4'-dimethoxytrityl chloride (DMTrCl, 5.9g, 17.4mmol) at 0°C under nitrogen.
The reaction system was stirred at room temperature for 3h and then quenched by addition
of water (1mL). The solvent was concentrated under reduced pressure. The residue was
purified by Flash column chromatography (0-10% methanol/ dichloromethane) to afford
intermediate 1-4 (7.4 g) as a yellow solid. m/z: [M+H]+ 704.2.
[0184] Step 5: To a solution of intermediate 1-4 (1.2g, 1.70mmol) in pyridine (5mL) was
added tert-butyldimethylsilyl chloride (TBSCl, 0.31g, 2.05mmol) and imidazole (0.29g,
4.30mmol) at 0°C under nitrogen. The reaction system was stirred at room temperature
for 16h, and then cooled with ice-water, diluted with water (10mL) and ethyl acetate
(50mL), the organic layer washed with brine (50mL×2), the organic layer was separated
and dried over anhydrous sodium sulfate, filtered, and concentrated under reduced
pressure. The residue was purified by Flash column chromatography (0-60% ethyl acetate/petroleum
ether) to afford intermediate 1-5 (less polar, 276mg, white solid) and 1-6 (more polar,
670mg, off-white solid). Intermediate 1-5: m/z: [M+H]+ 818.3; TLC R
f=0.42 (DCM/MeOH =15/1);
1H NMR (400 MHz, DMSO-
d6): δ 11.00 (s, 1H), 8.48 (s, 1H), 8.02 (d,
J=7.8 Hz, 2H), 7.62 (t,
J=7.4 Hz, 1H), 7.53 (t,
J=7.6 Hz, 2H), 7.39 (d,
J=7.8 Hz, 2H), 7.29-7.16 (m, 7H), 6.83 (d,
J=8.6 Hz, 4H), 5.90 (d,
J=5.8 Hz, 1H), 5.13 (d,
J=6.0 Hz, 1H), 5.08 (t,
J=5.5 Hz, 1H), 4.27 (dd,
J=9.6, 5.2 Hz, 1H), 4.07 (d,
J=4.0 Hz, 1H), 4.00 (s, 3H), 3.71 (s, 6H), 3.27-2.23 (m, 1H), 3.14-3.10 (m, 1H), 0.74
(s, 9H), 0.10 (d,
J=6.6 Hz, 6H). Intermediate 1-6: m/z: [M+H]
+ 818.3;TLC R
f=0.23 (DCM/MeOH =10/1);
1H NMR (400 MHz, DMSO-
d6): δ 11.00 (s, 1H), 8.50 (s, 1H), 8.01 (d,
J=7.6 Hz, 2H), 7.62 (m, 1H), 7.52 (m, 2H), 7.32 (d,
J=7.5 Hz, 2H), 7.26-7.16 (m, 7H), 6.82 (dd,
J=8.8, 2.3 Hz, 4H), 5.83 (d,
J=5.2 Hz, 1H), 5.40 (d,
J=6.1 Hz, 1H), 5.06 (m, 1H), 4.59 (t,
J=4.5 Hz, 1H), 4.06 (s, 3H), 3.71 (s, 6H), 3.35-3.33(m, 1H), 3.31-3.26 (m, 1H), 3.09-3.01
(m, 1H), 0.85 (s, 9H), 0.08 (d,
J=7.8 Hz, 6H).
[0185] Step 6: To a solution of intermediate 1-6 (400mg, 0.48mmol) in pyridine (4mL) was
added diphenyl phosphite (460mg, 1.92mmol) and then stirred at room temperature for
30min. Triethylamine (0.4mL) and water (0.4 mL) was successively added to the above
reaction system and stirred for 30min. Dicholormethane (5 mL) and aqueous solution
of sodium bicarbonate (5 mL, 5%) was successively added to the above reaction system.
The organic layer washed with water, and then the separated organic layer was dried
over anhydrous sodium sulfate, filtered and concentrated. The residue was purified
by Flash column chromatography (0-10% methanol/dichloromethane) to afford intermediate
1-7 (triethylamine salt, 600mg) as a white solid. m/z: [M+H]
+ 882.3.
[0186] Step 7: To a solution of intermediate 1-7 (2.8g, 2.85mmol) in a mixed solvent of
dichloromethane (20mL) and water (0.3mL) was added a dichloromethane solution of dichloroacetic
acid (DCA) (0.6M, 23.7mL), and then stirred at room temperature for 1h. To the reaction
solution was added pyridine (20mL), the resulting mixture was stirred at room temperature
for 10min. The solvent was concentrate under reduced pressure to afford intermediate
1-8 (pyridinium salt, curd product). m/z: [M+H]
+ 580.1.
Embodiment 2: Synthesis of compounds 1-p1, 1-p2, 1-p3 and 1-p4
[0187]

[0188] Step 1: Intermediate 1-8 (2.85mmol) was dissolved in anhydrous acetonitrile (15 mL)
and then the solvent was concentrated under reduced pressure, repeated twice and left
10mL acetonitrile at last time, 4A molecular sieve (0.8g) was added thereto. Compound
1-9 (
CASNo: 104992-55-4, 3.3 g, 3.42 mmol) was dissolved in anhydrous acetonitrile (15mL) and then the solvent
was concentrated under reduced pressure, repeated twice and left 5mL acetonitrile
at last time. To the solution of 1-8 in acetonitrile was slowly added the acetonitrile
solution of compound 1-9 at 0°C, the reaction system was stirred at room temperature
for 0.5h, ((Dimethylaminomethylidene)amino)-3
H-1,2,4-dithiazoline-3-thione (DDTT, 697mg, 3.42 mmol) was added thereto, and stirred
for additional 40min. The molecular sieve was removed by filtration, and the filtrate
was concentrated under reduced pressure to afford compound 1-10 (7.5g). m/z: [M+H]
+ 1499.3.
[0189] Step 2: Compound 1-10 (3.7g, 2.35mmol) was dissolved in dichloromethane (35mL) and
water (0.7mL); dichloromethane solution of DCA (0.6 M, 31mL, 18.8mmol) was dropped
thereto at room temperature. The reaction system was stirred at room temperature for
2h. Triethylsilane (20 mL) was dropped thereto, and the reaction solution was stirred
at room for additional 1h. Pyridine (10mL) was dropped thereto, the the reaction solution
was concentrated under reduced pressure. The residue was purified by
prep-HPLC to afford compound 1-11 (400 mg) as a white solid. m/z: [M+H]
+ 1196.2.
[0190] Step 3&4: To pyridine (5mL) was slowly added diphenyl chlorophosphate (DPCP, 1g,
3.8 mmol) dropwise at -40°C, to the above solution was slowly added anhydrous dichloromenthane
solution (5mL) of compound 1-11 (230mg, 0.19mmol) dropwise at -40°C, and then stirred
at this temperature for 30min, and obtained the reaction solution of compound 1-12.
To the solution of compound 1-12 was directly added 3
H-1,2-benzodithiol-3-one (64mg, 0.38mmol) and stirred for 1h. Water (68mg, 0.38mmol)
was added thereto and stirred for additional 1h. The reaction solution was diluted
with ethyl acetate and washed with aqueous solution of sodium bicarbonate (2.7%, 30
mL), the organic layer was separated and concentrated to afford compound 1-13. Compound
1-13 was separated by
prep-HPLC (separation method 2) to afford 3 isomers: 1-13-p1 (60mg) as a yellow solid,
1-13-p2 (30mg) as a white solid, and 1-13-p3 (40mg) as a white solid.
[0191] Step 5: To a solution of compound 1-13-p1 (80mg, 0.066mmol) in acetonitrile (2mL)
was added tert-butylamine (2mL). The reaction system was stirred at room temperature
for 0.5h. The solvent was concentrate under reduced pressure. The residue was dissolved
in methanol (4mL), methanolic hydrochloric acid solution (2M, 4mL) was added thereto,
the reaction solution was stirred at 45°C for 1h, and the solvent was concentrate
under reduced pressure to afford compound 1-14-p1 (100mg) as a yellow solid. m/z:
[M+H]
+ 1143.1.
[0192] Step 6: To a solution of compound 1-14-p1 (100mg) in methanol (6 mL) was added ammonium
hydroxide solution (6mL). The reaction system was stirred at 45°C for overnight. The
solvent was concentrate under reduced pressure. The residual liquid was lyophilized
to afford compound 1-15-p1 (100mg, crude compound) as a yellow solid. m/z: [M+H]
+ 935.2.
[0193] Step 7: Compound 1-15-p1 (45mg) was subjected to azeotropic dehydration three times
with anhydrous pyridine (10mL) and then dissolved in pyridine (2mL), and then triethylamine
(0.66mL) and triethylamine trihydrofluoride (387mg) was added thereto under nitrogen.
The resulting solution was stirred at 45°C for 3h. The solvent was concentrate under
reduced pressure. The residue was purified by
prep-HPLC (separation method 3) to afford compound 1-p1 (0.34mg, m/z: [M+H]
+ 706.8, HPLC-RT: 8.584min) and 1-p2 (0.30mg, m/z: [M+H]
+ 706.8, HPLC-RT: 8.662 min), as white solids.
Synthesis of compound 1-p3:
[0194] In a same way, to a solution of compound 1-13-p2 (50mg, 0.041mmol) in acetonitrile
(2.0 mL) was added tert-butylamine (2mL), and stirred at room temperature for 0.5h,
the solvent was concentrate under reduced pressure. The residue was dissolved in methanol
(1mL), methanolic hydrochloric acid solution (2mL, 2M) was added thereto, the reaction
solution was stirred at 40°C for 4h, and then the solvent was concentrate under reduced
pressure. The residue was dissolved in methanol (1mL), ammonium hydroxide solution
(1mL) was added thereto and stirred at 50°C for 16h, and then the reaction solution
was purged with nitrogen to remove most of the ammonia. The solvent was concentrate
under reduced pressure. The residual liquid was lyophilized. The crude compound was
subjected to azeotropic dehydration three times with anhydrous pyridine (10mL) and
then dissolved in pyridine (2mL), triethylamine (0.66mL) and triethylamine trihydrofluoride
(0.36mL) was added thereto under nitrogen. The resulting solution was stirred at 50°C
for 2h. The solvent was concentrate under reduced pressure. The residue was purified
by
prep-HPLC (separation method 3) to afford compound 1-p3 (0.40 mg, m/z: [M+H]
+ 706.8, HPLC-RT: 9.789 min) as an off-white solid.
Synthesis of compound 1-p4:
[0195] Compound 1-p4 (2.4mg, m/z: [M+H]
+ 706.8, HPLC-RT: 9.960 min) was obtained as a white solid in the same manner as compound
1-p3, by using compound 1-13-p3 (40mg, 0.033 mmol) as a starting material.
1H NMR (400 MHz, DMSO-
d6+D
2O): δ 8.37(s, 1H), 8.16(s,1 H), 7.97(s, 1H), 5.93 (d,
J=7.8 Hz, 1H), 5.74 (d,
J=8.0, 1H), 5.35 (m, 1H), 5.14 (m, 1H), 4.70 (m, 1H), 4.59 (s, 1H), 4.18 (s, 1H), 4.15
(m, 1H), 3.85 (m, 2H), 3.53 (m, 1H), 3.06 (m,1H);
31P NMR (161 MHz, DMSO-
d6+D
2O): δ 58.47, 46.58.
Embodiment 3: Synthesis of intermediates 2-8 and 2-9
[0196]

[0197] Step 1: To a solution of 7-aminothiazolo[4,5-
d]pyrimidin-2(3
H)-one (refer to
J. Med. Chem. 1990, 33, 407-415, compound 28) (6.5g, 38.7mmol) and tetraacetylribose (11g, 46.4mmol) in acetonitrile
(120mL) was added
N,O-bis(trimethylsilyl)acetamide (BSA, 13.6g, 116mmol), and then stirred at reflux for
1h. To the above reaction solution was added trimethylsilyl trifluoromethylsulphonate
(TMSOTf, 17.2g, 77.4mmol) after cooling to room temperature, and stirred at reflux
for additional 48h. The reaction solution was cooled to room temperature, and saturated
aqueous solution of sodium bicarbonate was slowly added thereto, the mixture was extracted
with ethyl acetate (150mL×3), the combined organic layers were washed with water,
the separated organic layer was dried over anhydrous sodium sulfate, filtered and
concentrated. The residue was purified by Flash column chromatography (ethyl acetate/petroleum
ether=3/4) to afford intermediate 2-1 (4.3g) as a light yellow solid. m/z: [M+H]
+ 427.0.
[0198] Step 2: To a solution of intermediate 2-1 (0.5g, 1.17mmol) in pyridine (5mL) was
added chlorotrimethylsilane (0.07mL, 0.58mmol) at 0°C under nitrogen, and stirred
at for 5min, to the above reaction solution was added benzoyl chloride (0.32mL, 2.81mmol),
the reaction system was stirred at room temperature for overnight, and then quenched
by addition of water (50mL). The aqueous layer was extracted with ethyl acetate (50mL×2),
the combined organic layers were washed with water, and the separated organic layer
was dried over anhydrous sodium sulfate, filtered and concentrated. The residue was
purified by Flash column chromatography (ethyl acetate/ petroleum ether =9/10) to
afford intermediate 2-2 (0.45g) as a light yellow solid. m/z: [M+H]
+ 634.8.
[0199] Step 3: To a solution of intermediate 2-2 (3g, 4.7mmol) in acetonitrile (150mL) was
added aqueous solution of lithium hydroxide (47mL, 1M), the reaction system was stirred
at room temperature for 15min and then neutralized pH to 6 with hydrochloric acid
(2M). The reaction solution was concentrated to 1/3 of the total volume, the solid
was precipitated, filtered, the filter cake washed with water for 3 times, and then
dried under vacuum to afford intermediate 2-3 (1g) as a yellow solid. m/z:[M+H]
+ 405.0.
[0200] Step 4: To a solution of intermediate 2-3 (7.6g, 18.8mmol) in pyridine (95mL) was
added DMTrCl (9.5g, 28.5mmol) under nitrogen. The reaction system was stirred at room
temperature for overnight. The solvent was concentrate under reduced pressure. The
residue was purified by Flash column chromatography (methanol/dichloromethane=1/25)
to afford intermediate 2-4 (8.3g) as an off-white solid. m/z: [M+H]
+ 706.8.
[0201] Step 5: To a solution of intermediate 2-4 (8.3g, 11.7mmol) and imidazole (2g, 29.3mmol)
in pyridine (60mL) was added TBSCl (2.1g, 14mmol) at 0°C under nitrogen. The reaction
system was stirred at room temperature for overnight, and then quenched by addition
of water (1mL) and saturated aqueous solution of sodium bicarbonate (100mL), the aqueous
layer was extracted with ethyl acetate (200mL×2), the combined organic layers were
washed with water, the organic layer was dried over anhydrous sodium sulfate, filtered
and concentrated, the residue was purified by Flash column chromatography (methanol/dichloromethane=1/50∼1/20)
to afford intermediate 2-5 (4g, off-white solid, LCMS-RT (Thermo): 2.813 min) and
2-6 (2g, off-white solid, LCMS-RT (Thermo): 2.763 min. Intermediate 2-5: m/z:[M+H]
+821.1;
1H NMR (400 MHz, DMSO-
d6): δ 11.71 (s, 1H), 8.66 (s, 1H), 8.05-8.03 (m, 2H), 7.68-7.64 (m, 1H), 7.56-7.52
(m, 2H), 7.43-7.32 (m, 2H), 7.27-7.17 (m, 7H), 6.84-6.82 (m, 4H), 6.05-6.04 (m, 1H),
5.29-5.28 (m, 1H), 4.88-4.84 (m, 1H), 4.53-4.50 (m, 1H), 4.05-3.98 (m, 1H), 3.71 (s,
6H), 3.35-3.27 (m, 1H), 3.08-3.04 (m, 1H), 0.82 (s, 9H), 0.05 (s, 3H), 0.01 (s, 3H);
Intermediate 2-6: m/z:[M+H]
+821.1;
1H NMR (400 MHz, DMSO-
d6): δ 11.73 (s, 1H), 8.62 (s, 1H), 8.06-8.04 (m, 2H), 7.68-7.65 (m, 1H), 7.57-7.53(m,
2H), 7.41-7.36 (m, 2H), 7.27-7.17 (m, 7H), 6.85-6.82(m, 4H), 6.09-6.08 (m, 1H), 5.00-4.95
(m, 2H), 4.31-4.27 (m, 1H), 4.05-4.03 (m, 1H), 3.72 (s, 6H), 3.23-3.16 (m, 2H), 0.95
(s, 9H), 0.05 (s, 6H).
[0202] Step 6: To a solution of intermediate 2-5 (0.5g, 0.61mmol) in pyridine (5mL) was
added diphenyl phosphite (0.57g, 1.43mmol) at 0°C under nitrogen, the reaction system
was stirred for 1h, and then triethylamine (0.6mL) and water (0.6mL) was added thereto.
The resulting mixture was stirred at room temperature for 5min and then diluted with
water (50mL), the aqueous layer was extracted with dicholormethane (30mL×2), the combined
organic layers were washed with water, and the separated organic layer was dried over
anhydrous sodium sulfate, filtered and concentrated. The residue was purified by Flash
column chromatography (methanol/dichloromethane=1/10) to afford intermediate 2-7 (triethylamine
salt, 0.6g) as an off-white solid. m/z: [M+H]
+ 884.5.
[0203] Step 7: To a solution of intermediate 2-7 (0.6g, 0.61mmol) in a mixed solvent of
dichloromethane (10mL) and water (1mL) was added a dichloromethane solution of DCA
(0.6 M, 9.1mL). The reaction mixture was stirred for 0.5h, and then pyridine (20mL)
was added thereto, the resulting mixture was concentrate under reduced pressure to
afford intermediate 2-8 (pyridinium salt, curd product). m/z: [M+H]
+ 582.9.
[0204] Synthesis of intermediate 2-9: intermediate 2-9 (pyridinium salt) was obtained in
the same manner as intermediate 2-8, by using intermediate 2-6 as a starting material.
m/z:[M+H]
+583.0.
Embodiment 4: Synthesis of compounds 2-p1, 2-p2, 2-p3 and 2-p4
[0205]

[0206] Step 1: Intermediate 2-8 (0.68mmol) and 1-9 (0.81g, 0.82mmol) were subjected to azeotropic
dehydration twice with anhydrous acetonitrile (10mL) respectively, and then dissolved
in acetonitrile (5mL) respectively for use. To the solution of intermediate 2-8 in
acetonitrile, which contained 4A molecular sieve, was slowly added the acetonitrile
solution of intermediate 1-9 at 0°C under nitrogen, the resulting mixture was stirred
for 1h. To the above reaction system was added DDTT (0.16g, 0.79mmol) and stirred
for additional 1h. The molecular sieve was removed by filtration, and the filtrate
was concentrated under reduced pressure to afford compound 2-9 (1g). m/z: [M+H]
+ 1501.5.
[0207] Step 2: To the solution of compound 2-9 (0.33g, 0.22mmol) in a mixed solvent of dichloromethane
(3mL) and water (0.3mL) was added dichloromethane solution of DCA (0.6 M, 2.93mL)
under nitrogen. The reaction system was stirred for 0.5h, and then pyridine (1mL)
was added thereto and concentrated under reduced pressure, and the residue was purified
by Flash column chromatography (acetonitrile/ aqueous solution of ammonium bicarbonate
(10mmol/L) =60%) to afford compound 2-10 (0.25g, pyridinium salt) as a white solid.
m/z: [M+H]+ 1199.6.
[0208] Step 3&4: Compound 2-10 (250mg, 0.21mmol) was subjected to azeotropic dehydration
three times with pyridine (1mL) and then dissolved in a mixed solvent of pyridine
(2mL) and dichloromethane (2mL). 2-chloro-5,5-dimethyl-1,3,2-dioxaphosphorinane 2-oxide
(DMOPC) (775mg, 4.2mmol) was added thereto, the mixture was stirred at room temperature
for 10min to obtain the reaction solution of compound 2-11. To the above reaction
solution of compound 2-11 was directly added water (756mg, 42mmol) and 3
H-1,2-benzodithiol-3-one (71mg, 0.42mmol), and stirred at room temperature for 20min,
aqueous solution of sodium bicarbonate (2.7%, 50mL) was added thereto. The aqueous
layer was extracted with ethyl acetate, and the separated organic layer was concentrated.
The residue was purified by Flash column chromatography (acetonitrile/ aqueous solution
of ammonium bicarbonate (10mmol/L) =10-80%) to afford compound 2-12-p1 (30mg, LCMS-RT(Thermo):
2.147min), 2-12-p2 (35 mg, LCMS-RT(Thermo): 2.247min), and 2-12-p3 (50mg, LCMS- RT(Thermo):
2.327min), as white solids.
[0209] Step 5: To a solution of compound 2-12-p1 (30mg, 0.01mmol) in acetonitrile (2mL)
was added tert-butylamine (2mL), the reaction system was stirred at room temperature
for 0.5h, and then directly concentrated to afford compound 2-13-p1 (30mg, crude product).
m/z: [M+H]+ 1159.9.
[0210] Step 6: To a solution of compound 2-13-p1 (30mg, crude) in methanol (2mL) was added
ammonium hydroxide solution (2mL), the reaction system was stirred at 45°C for overnight
in a sealed tube, and then the reaction solution was concentrated in reduced pressure.
The residue was subjected to azeotropic dehydration three times with pyridine (2mL)
and then dissolved in pyridine (2mL). To the above reaction system was added triethylamine
(0.35g, 3.5mmol) and triethylamine trihydrofluoride (0.28g, 1.75mmol) under nitrogen.
The resulting mixture was stirred at 45°C for 6h. The solvent was concentrate under
reduced pressure. The residue was adjust pH to 8 with aqueous solution of ammonium
bicarbonate (1M), and then purified by
prep-HPLC (separation method 3) to afford compound 2-p1 (di-ammonium salt, 2mg, m/z: [M+H]
+ 723.8, HPLC-RT: 8.116min) and 2-p2 (di-ammonium salt, 2mg, m/z: [M+H]
+ 723.8, HPLC-RT: 10.121min), as white solids.
Synthesis of compound 2-p3:
[0211] To a solution of compound 2-12-p2 (30mg, 0.01mmol) in acetonitrile (2mL) was added
tert-butylamine (2mL). The mixture was stirred at room temperature for 0.5h, and then
concentrated under reduced pressure to afford compound 2-13-p2. To a solution of compound
2-13-p2 in methanol (2mL) was added ammonium hydroxide solution (2mL), the reaction
system was stirred in a sealed tube at 45°C for overnight, and then the reaction solution
was directly concentrated under reduced pressure. The residue was subjected to azeotropic
dehydration three times with pyridine (2mL) and then dissolved in pyridine (2mL).
To the above reaction system was added triethylamine (0.35g, 3.5mmol) and triethylamine
trihydrofluoride (0.28g, 1.75mmol) under nitrogen. The resulting mixture was stirred
at 45°C for 6h. The solvent was concentrate under reduced pressure. The residue was
adjust pH to 8 with aqueous solution of ammonium bicarbonate (1M), and then purified
by
prep-HPLC (separation method 3) to afford compound 2-p3 (di-ammonium salt, 0.3mg, m/z:
[M+H]
+ 723.7, HPLC-RT: 10.121min) as a white solid.
Synthesis of compound 2-p4:
[0212] Compound 2-p4 (di-ammonium salt, 0.4 mg, m/z: [M+H]
+ 723.7, HPLC-RT: 9.632 min) was obtained as a white solid in the same manner as compound
2-p3, by using compound 2-12-p3 (17mg, 0.01mmol) as a starting material.
Embodiment 5: Synthesis of compounds 3-p1, 3-p1/3-p2 and 3-p3
[0213]

[0214] Step 1: Compound 3-1 (
CAS No.: 129451-95-8) (2.36g, 2.4mmol) and Intermediate 2-9 (1.05g, 2mmol) were subjected to azeotropic
dehydration twice with anhydrous acetonitrile (10mL) respectively, and then dissolved
in acetonitrile (5mL) respectively for use. To the solution of intermediate 2-9 in
acetonitrile, which contained 4A molecular sieve, was slowly added the acetonitrile
solution of compound 3-1 at 0°C under nitrogen, the resulting mixture was stirred
for 1h, To the above reaction system was added DDTT (0.49g, 2.4mmol), and stirred
for additional 1h. The molecular sieve was removed by filtration, and the filtrate
was concentrated under reduced pressure to afford compound 3-3 (1.3g). m/z: [M+H]
+ 1501.5.
[0215] Step 2: To the solution of compound 3-3 (1g, 0.66mmol) in a mixed solvent of dichloromethane
(3mL) and water (0.3mL) was added dichloromethane solution of DCA (0.6 M, 10mL). The
reaction system was stirred for 0.5h. Pyridine (3mL) was added thereto, the mixture
was concentrated under reduced pressure. The residue was purified by Flash column
chromatography (acetonitrile/ aqueous solution of ammonium bicarbonate (10mmol/L)
=60%) to afford compound 3-4 (390mg, pyridinium salt) as a white solid. m/z: [M+H]
+ 1199.6.
[0216] Step 3&4: Compound 3-4 (250mg, 0.21mmol) was subjected to azeotropic dehydration
three times with pyridine (1mL) and then dissolved in a mixed solvent of pyridine
(2mL) and dichloromethane (2mL). To the above reaction system was added DMOPC (775
mg, 4.2mmol), the mixture was stirred at room temperature for 10min to obtain the
reaction solution of compound 3-5. To the reaction solution of compound 3-5 was added
water (756mg, 42mmol) and 3H-1,2-benzodithiol-3-one (71mg, 0.42mmol) and stirred at
room temperature for 20min, and then aqueous solution of sodium bicarbonate (2.7%,
50mL) was added thereto, the aqueous layer was extracted with ethyl acetate, and the
separated organic layer was concentrated. The residue was purified by Flash column
chromatography (acetonitrile/ aqueous solution of ammonium bicarbonate (10mmol/L)=10-80%)
to afford compound 3-6-p1 (50mg, LCMS-RT(Thermo): 2.143min), 3-6-p2 (15mg, LCMS-RT(Thermo):
2.230min), and 3-6-p3 (18mg, LCMS- RT(Thermo): 2.320), as white solids.
[0217] Step 5: To a solution of compound 3-6-p1 (20mg, 0.02mmol) in acetonitrile (2mL) was
added tert-butylamine (2mL), the reaction system was stirred at room temperature for
0.5h, and then directly concentrated to afford compound 3-7-p1 (25mg, crude product).
m/z: [M+H]
+ 1159.9.
[0218] Step 6: To a solution of compound 3-7-p1 (25mg, crude) in methanol (2mL) was added
ammonium hydroxide solution (2mL), the reaction system was stirred in a sealed tube
at 45°C for overnight, and then the reaction solution was concentrated in reduced
pressure. The residue was subjected to azeotropic dehydration three times with pyridine
(2mL) and then dissolved in pyridine (2mL). To the above reaction system was added
triethylamine (0.35g, 3.5mmol) and triethylamine trihydrofluoride (0.28g, 1.75mmol)
under nitrogen. The resulting mixture was stirred at 50°C for 6h. The solvent was
concentrate under reduced pressure. The residue was adjust pH to 8 with aqueous solution
of ammonium bicarbonate (1M), and then purified by
prep-HPLC (separation method 3) to afford compound 3-p1 (di-ammonium salt, 0.7mg, m/z:
[M+H]+ 723.8, HPLC-RT: 9.726min) and a mixture of 3-p1/3-p2 (di-ammonium salt, 1.1mg,
m/z: [M+H]
+ 723.8, HPLC- RT: 9.726 min and 11.161min).
Synthesis of compound 3-p3:
[0219] To a solution of compound 3-6-p3 (50mg, 0.04mmol) in acetonitrile (2mL) was added
tert-butylamine (2mL). The mixture was stirred at room temperature for 0.5h, and then
concentrated under reduced pressure to afford compound 3-7-p3. To a solution of compound
3-7-p3 in methanol (2mL) was added ammonium hydroxide solution (2mL), the reaction
system was stirred in a sealed tube at 45°C for overnight, and then the reaction solution
was directly concentrated under reduced pressure. The residue was subjected to azeotropic
dehydration three times with pyridine (2mL) and then dissolved in pyridine (2mL).
To the above reaction system was added triethylamine (0.35g, 3.5mmol) and triethylamine
trihydrofluoride (0.28g, 1.75mmol) under nitrogen. The resulting mixture was stirred
at 45°C for 6h. The solvent was concentrate under reduced pressure. The residue was
adjust pH to 8 with aqueous solution of ammonium bicarbonate (1M), and then purified
by
prep-HPLC (separation method 3) to afford compound 3-p3 (di-ammonium salt, 2.2mg, m/z:
[M+H]
+ 723.7, HPLC-RT: 11.76min) as a white solid.
Embodiment 6: Synthesis of intermediate 4-3
[0220]

[0221] Step 1: To a solution of 2'-fluoro-2'-deoxyadenosine (
CAS No.: 64183-27-3) (9.7g, 36.0mmol) in pyridine (110mL) was added chlorotrimethylsilane (23.5g, 216mmol)
at 0°C under nitrogen. The mixture was stirred at room temperature for 2h, and then
benzoyl chloride (7.6g, 54 mmol) was added thereto. The resulting mixture was stirred
at room temperature for overnight. To the reaction solution was added water (40mL)
and stirred for 1h, and then ammonium hydroxide solution (40mL) was added thereto
and stirred for additional 2h. Additional water (40mL) was added thereto, the mixture
was extracted with ethyl acetate (500mL×2), and the combined organic layers were dried
over anhydrous sodium sulfate, and then concentrated to 1/5 of the total volume, filtered,
the filter cake was dried under vacuum to afford intermediate 4-1 (10g) as a white
solid. m/z: [M+H]
+ 374.0.
[0222] Step 2: To a solution of intermediate 4-1 (1.2g, 3.2mmol) in pyridine (15mL) was
added DMTrCl (1.6g, 4.8mmol) under nitrogen. The reaction system was stirred at room
temperature for overnight. To the reaction solution was added water (50mL), the mixture
was extracted with ethyl acetate (40mL×3), and the combined organic layers were dried
over anhydrous sodium sulfate and concentrated under reduced pressure. The residue
was purified by Flash column chromatography (2% methanol/dichloromethane) to afford
intermediate 4-2 (1.9g) as a light yellow solid. m/z: [M+H]
+ 676.0.
[0223] Step 3: To a solution of intermediate 4-2 (1.35g, 2mmol) in dichloromethane (10mL)
was added
N,N-diisopropylethylamine (1.1mL, 6mmol) and 2-cyanoethyl
N,N-diisopropylchloro- phosphoramidite (947mg, 4mmol) at 0°C under nitrogen. The resulting
mixture was stirred at room temperature for 2h. To the reaction solution was added
water (50mL) and saturated aqueous solution of sodium bicarbonate (20mL). The resulting
mixture was extracted with ethyl acetate (50mL×3), and the combined organic layers
were dried over anhydrous sodium sulfate, filtered and concentrated under reduced
pressure. The residue was purified by Flash column chromatography (3% methanol/ dichloromethane)
to afford intermediate 4-3 (1.2g) as a yellow solid. m/z: [M+H]
+ 876.1.
Embodiment 7: Synthesis of compounds 4-p1, 4-p2 and 4-p3
[0224]

[0225] Step 1: To a solution of intermediate 4-4 (4-4 was obtained as a mixture of stereoisomers
in the same manner as Embodiment 4 steps 1-4, by using intermediates 4-3 and 2-8 as
starting materials) (50mg, 0.05mmol) in acetonitrile (2mL) was added tert-butylamine
(2mL). The resulting mixture was stirred at room temperature for 0.5h, and then concentrated
under reduced pressure. The residue was purified by Flash column chromatography (acetonitrile/
aqueous solution of ammonium bicarbonate (10mmol/L) =10-80%) to afford compound 4-5
(15mg) as a white solid. m/z: [M+H]
+ 1047.8.
[0226] Step 2: To the solution of compound 4-5 (30mg, 0.03mmol) in methanol (2mL) was added
ammonium hydroxide solution (2mL), the reaction solution was stirred in a sealed tube
at 45°C for overnight, and then the solvent was concentrated in reduced pressure.
The residue was subjected to azeotropic dehydration three times with anhydrous pyridine
(2mL) and then dissolved in pyridine (1mL), and then triethylamine (0.91g, 9mmol)
and triethylamine trihydrofluoride (0.58g, 3.6mmol) was added thereto under nitrogen.
The resulting mixture was stirred at 50°C for 6h. The solvent was concentrate under
reduced pressure. The residue was adjusted pH to 8 with ammonium hydroxide solution,
and then directly purified by
prep-HPLC (separation method 3) to afford compound 4-p1 (di-ammonium salt, 0.76mg, m/z:
[M+H]
+ 725.8, HPLC-RT: 9.04min), 4-p2 (di-ammonium salt, 0.82mg, m/z: [M+H]
+ 725.8, HPLC-RT: 10.45min), and 4-p3 (di-ammonium salt, 0.97mg, m/z: [M+H]
+ 725.8, HPLC-RT: 10.35min), as white solids.
Embodiment 8: Synthesis of compounds 5-p1, 5-p2, 5-p3 and 5-p4
[0227]

[0228] Step 1: To a solution of compound 5-1-p1 (5-1-p1, 5-2-p2 and 5-3-p3 were obtained
in the same manner as Embodiment 4 steps 1-4, by using intermediates 3-2 and 3'-TBDMS-IBU-RG
phosphoramidite (
CASNo: 1445905-51-0) as starting materials, LCMS- RT(Thermo) of 5-1-p1, 5-2-p2 and 5-3-p3 were 2.11min,
2.14min and 2.31min, respectively) (30mg, 0.03mmol) in acetonitrile (2mL) was added
tert-butylamine (2mL). The resulting mixture was stirred at room temperature for 0.5h,
and then concentrated to afford compound 5-2-p1 (30mg, crude product). m/z: [M+H]
+ 1141.6.
[0229] Step 2: To the solution of compound 5-2-p1 (30mg, crude) in methanol (2mL) was added
ammonium hydroxide solution (2mL), the reaction solution was stirred in a sealed tube
at 45°C for overnight, and then the solvent was concentrated in reduced pressure.
The residue was subjected to azeotropic dehydration three times with anhydrous pyridine
(2mL) and then dissolved in pyridine (1mL), and then triethylamine (0.35g, 3.5mmol)
and triethylamine trihydrofluoride (0.28g, 1.75mmol) was added thereto under nitrogen.
The resulting mixture was stirred at 50°C for 6h. The solvent was concentrate under
reduced pressure. The residue was adjusted pH to 8 with ammonium hydroxide solution,
and then directly purified by
prep-HPLC (separation method 3) to afford compound 5-p1 (di-ammonium salt, 0.89mg, m/z:
[M+H]
+ 739.8, HPLC-RT: 6.495min) as a white solid.
Synthesis of compounds 5-p2 and 5-p3
[0230] Compounds 5-p2 (di-ammonium salt, 13mg, m/z: [M+H]
+ 739.8, HPLC-RT: 10.666min,
1H NMR (400 MHz, DMSO-
d6+D
2O): δ 8.22 (s, 1H), 8.10 (s, 1H), 5.99 (d,
J=7.7 Hz, 1H), 5.85 (d,
J=8.5 Hz, 1H), 5.34-5.28 (m, 1H), 5.21-5.25 (m, 1H), 5.12-5.16 (m, 1H), 4.43-4.31 (m,
1H), 4.16-4.12 (m, 1H), 4.08-4.06 (m, 1H), 4.01-3.97 (m, 2H), 3.66 (d,
J=11.8 Hz, 1H), 3.59-3.55 (m, 1H);
31P NMR (162 MHz, DMSO-
d6): δ 59.36, 57.52) and 5-p3 (di-ammonium salt, 0.7mg, m/z: [M+H]
+ 739.9, HPLC-RT: 10.663min) were obtained as white solids in the same manner as compound
5-p1, by using compound 5-1-p2 (76.5mg, crdue) as a starting material.
Synthesis of compound 5-p4
[0231] Compounds 5-p4 (di-ammonium salt, 1.41mg, m/z: [M+H]
+ 739.9, HPLC-RT: 11.973min) was obtained as a white solid in the same manner as compound
5-p1, by using compound 5-1-p3 (100 mg, crude) as a starting material.
Embodiment 9: Synthesis of intermediate 6-14
[0232]

[0233] Step 1: Tetraacetylribose (150g, 472mmol) was dissolved in acetone (1L), and iodine
(11.9g, 47.2mmol) was added thereto at 0°C. After addition, the reaction system was
stirred at room temperature for 12h. The saturated aqueous solution of sodium bisulfate
was added into the reaction solution to remove the excess iodine, and then extracted
with ethyl acetate (500mL×3), and the combined organic layers were washed with brine,
the separated organic layer was dried over anhydrous sodium sulfate, the filtrate
was concentrated under reduced pressure. The residue was purified by Flash column
chromatography (0-60% ethyl acetate/ petroleum ether) to afford intermediate 6-1 (100g)
as a yellow oil. m/z: [M+H]
+ 275.0.
[0234] Step 2: To the suspension of intermediate 6-1 (100g, 365mmol) in methanol (100mL)
was added potassium carbonate (150g, 1.09mmol). The reaction system was stirred at
room temperature for 12h, and then filtered, the filtrate was concentrated under reduced
pressure. The residue was purified by Flash column chromatography (0-10% methanol/dichloromethane)
to afford intermediate 6-2 (60.3g) as a white solid. m/z: [M+H]
+ 191.0.
[0235] Step 3: To a solution of intermediate 6-2 (60g, 316mmol) in pyridine (300mL) was
added DMTrCl (128g, 379mmol) in small portions at 0°C under nitrogen. The reaction
system was stirred at room temperature for 12h and then quenched by addition of water
(100mL). The solvent was concentrate under reduced pressure. The residue was purified
by Flash column chromatography (0-50% petroleum ether/ ethyl acetate) to afford intermediate
6-3 (150.3g) as a white solid.
[0236] Step 4: To a solution of intermediate 6-3 (150g, 305mmol) in DMF (500mL) was added
sodium hydride (18.3g, 458mmol, 60%) under nitrogen at 0°C. The reaction system was
stirred at 0°C for 0.5h. To the reaction system was added iodomethane (56.3g, 396mmol)
and stirred at room temperature for 3h. The mixture was diluted with water (100mL)
and ethyl acetate (300mL × 3). The organic layer washed with brine, and then separated
and dried over anhydrous sodium sulfate, the filtrate was concentrated under reduced
pressure to afford intermediate 6-4 (150 g) as a white solid. m/z: [M+H]
+507.1.
[0237] Step 5: To a solution of intermediate 6-4 (150g, 296mmol) in dichloromethane (500mL)
was added DCA (344g, 2.67mol) at 0°C. The reaction system was stirred at 0°C for 3h,
and then quenched by addition of saturated aqueous solution of sodium carbonate, the
aqueous layer was extracted with dichloromethane (300mL×3), the combined organic layers
were washed with brine (150mL×2), the separated organic layer was dried over anhydrous
sodium sulfate, and then concentrated under reduced pressure. The residue was purified
by Flash column chromatography (0-10% methanol/dichloromethane) to afford intermediate
6-5 (50.2g) as a white solid. m/z: [M+H]
+ 205.0.
[0238] Step 6: To a solution of intermediate 6-5 (50g, 245mmol) in dichloromethane (500mL)
was added benzoyl chloride (41.2g, 294mmol) at 0°C. The reaction system was stirred
at room temperature for 12h, and then the reaction was quenched by addition of water,
the aqueous layer was extracted with dichloromethane (150mL×3), the combined organic
layers were washed with brine (150mL×2), the separated organic layer was dried over
anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue
was purified by Flash column chromatography (0-70% petroleum ether/ethyl acetate)
to afford intermediate 6-6 (65.3g) as a white solid. m/z: [M+H]
+ 309.0.
[0239] Step 7: Intermediate 6-6 (65g, 211mmol) was dissolved in an aqueous solution of trifluoroacetic
acid (150mL, 80%), the reaction system was stirred at room temperature for 5h. Most
of the solvent was removed under reduced pressure, and then the reaction solution
washed with saturated aqueous solution of sodium bicarbonate, the aqueous layer was
extracted with dichloromethane (100mL×3), the organic layer washed with brine (150
mL×2), the separated organic layer was dried over anhydrous sodium sulfate and concentrated
under reduced pressure to afford intermediate 6-7 (50.2g) as an off-white solid.
[0240] Step 8: To a solution of intermediate 6-7 (50g, 187mmol) in pyridine (150mL) was
slowly added acetic anhydride (114g, 1.11mol), the reaction system was stirred at
room temperature for 12h. Most of the solvent was removed under reduced pressure,
and then the reaction solution washed with saturated aqueous solution of sodium bicarbonate,
the aqueous layer was extracted with ethyl acetate (100mL×3), the combined organic
layers were washed with brine (100mL×2), the separated organic layer was dried over
anhydrous sodium sulfate, filtered, the filtrate was concentrated under reduced pressure.
The residue was purified by Flash column chromatography (0-70% petroleum ether/ ethyl
acetate) to afford intermediate 6-8 (60.2g) as an off-white solid.
[0241] Step 9: To a solution of 7-aminothiazolo[4,5-
d]pyrimidin-2(3
H)-one (6.5g, 38.7mmol) and intermediate 6-8 (16.3g, 46.4mmol) in acetonitrile (120mL)
was added BSA (13.6g, 116mmol), the reaction system was stirred at reflux for 1h.
To the reaction solution was added TMSOTf (17.2g, 77.4mmol) after the reaction solution
was cooled to room temperature and stirred at reflux for additional 72h, and then
to the reaction solution was slowly added saturated aqueous solution of sodium bicarbonate
after the reaction solution was cooled to room temperature, and then the aqueous layer
was extracted with ethyl acetate (150mL×3), the combined organic layers were washed
with water, the separated organic layer was dried over anhydrous sodium sulfate, filtered
and concentrated. The residue was purified by Flash column chromatography (ethyl acetate/
petroleum ether=3/4) to afford intermediate 6-9 (5g) as a light yellow solid. m/z:
[M+H]
+461.0.
[0242] Step 10: To a solution of intermediate 6-9 (5g, 10.9mmol) in pyridine (5mL) was added
benzoyl chloride (5.3g, 38mmol) at 0°C. The reaction system was stirred at room temperature
for overnight, and then quenched by addition of water (50mL), the aqueous layer was
extracted with dichloromethane (150mL×3), the combined organic layers were washed
with water, the separated organic layer was dried over anhydrous sodium sulfate, filtered
and concentrated. The residue was purified by Flash column chromatography (petroleum
ether/ethyl acetate=1/1) to afford intermediate 6-10 (6.8g) as a white solid. m/z:
[M+H]
+ 669.1.
[0243] Step 11: To a solution of intermediate 6-10 (6.8g, 10.1mmol) in a mixed solvent of
tetrahydrofuran and methanol (60/20mL) was added aqueous solution of lithium hydroxide
(36 mL, 1M) at 0°C, the reaction system was stirred at 0°C for 2h and then neutralized
pH to 6 with acetic acid. The reaction solution was concentrated to 1/3 of the total
volume, the solid was precipitated, filtered, the filter cake washed with water for
3 times, and then dried under vacuum to afford intermediate 6-11 (3.8g) as a yellow
solid. m/z:[M+H]
+ 419.0.
[0244] Step 12: To a solution of intermediate 6-11 (3.8g, 9.09mmol) in pyridine (60mL) was
added DMTrCl (3.6g, 10.9mmol) under nitrogen. The reaction system was stirred at room
temperature for overnight. The solvent was concentrate under reduced pressure. The
residue was purified by Flash column chromatography (petroleum ether/ethyl acetate=1/1)
to afford intermediate 6-12 (6g) as a light yellow solid.
[0245] Step 13: To a solution of intermediate 6-12 (5g, 6.94mmol) in pyridine (5mL) was
added diphenyl phosphite (4.9g, 20.8mmol), the reaction solution was stirred at room
temperature for 1h. Triethylamine (2mL) and water (1mL) was successively added thereto.
The reaction solution was stirred at room temperature for 5min, and then diluted with
water (50mL) and extracted with dicholormethane (60mL×3), the combined organic layers
were washed with aqueous solution of sodium bicarbonate (5%), the separated organic
layer was dried over anhydrous sodium sulfate, filtered and concentrated. The residue
was purified by Flash column chromatography (methanol/dichloromethane=1/10) to afford
intermediate 6-13 (5.1g, triethylamine salt) as an off-white solid. m/z: [M+H]
+ 886.3.
[0246] Step 14: To a solution of intermediate 6-13 (3g, 3.39mmol) in dichloromethane (20mL)
was added a dichloromethane solution of DCA (0.6M, 50mL) at 0°C. The reaction mixture
was stirred at room temperature for 1h and then triethylsilane (1mL) and pyridine
(3mL) were added thereto, the resulting mixture was stirred at room temperature for
additional 10min, the solvent was concentrated under reduced pressure to afford intermediate
6-14 (2.5g, pyridinium salt).
Embodiment 10: Synthesis of compounds 6-p1, 6-p2, 6-p3 and 6-p4
[0247]

[0248] Step 1: To a solution of compound 6-15 (6-15 was obtained as a mixture of stereoisomers
in the same manner as Embodiment 4 steps 1-4, by using intermediates 6-14 and 4-3
as starting materials) (50mg, 0.05mmol) in acetonitrile (2mL) was added tert-butylamine
(2mL). The resulting mixture was stirred at room temperature for 0.5h, and then concentrated
under reduced pressure to afford compound 6-16 (40mg). m/z: [M+H]
+ 948.0.
[0249] Step 2: To a solution of compound 6-16 (40mg, crude) in methanol (1mL) was added
ammonium hydroxide solution (1mL), the mixture was stirred in a sealed tube at 50°C
for 5h, and then cooled to room temperature and quenched by addition of acetic acid,
the mixture was lyophilized. The residue was purified by
prep-HPLC (separation method 4) to afford compound 6-p1 (di-ammonium salt, 3.6mg, m/z:
[M+H]
+ 740.0, HPLC-RT: 10.814min), 6-p2 (di-ammonium salt, 3.2mg, m/z: [M+H]
+ 740.0, HPLC-RT: 11.380min), 6-p3 (di-ammonium salt, 3.9mg, m/z: [M+H]
+ 740.0, HPLC-RT: 10.370min;
1H NMR (400 MHz, D
2O): δ 8.36 (s, 2H), 8.12(s, 1H), 6.61 (s, 1H), 6.11 (s, 1H), 5.79 (s, 1H), 5.39 (d,
J=51.6 Hz, 1H),4.93-5.02 (m, 1H), 4.36-4.48 (m, 3H), 3.84-4.03(m, 4H),3.51 (s, 3 H);
31P NMR (162 MHz, D
2O): δ 56.49, 51.13;
19F NMR (162 MHz, D
2O): δ -202.92.) and 6-p4 (di-ammonium salt, 2.3mg, m/z: [M+H]
+ 740.0, HPLC-RT: 11.650min), as white solids.
Embodiment 11: Synthesis of intermediate 7-1
[0250]

[0251] Intermediate 7-1 was obtained as a yellow solid in the same manner as Embodiment
3 intermediate 2-8, by using tetraacetylribose and 5-aminothiazolo[4,5-
d]pyrimidine-2,7(3
H,6
H)-dione (refer to
J. Med. Chem. 1990, 33, 407-415, compound 4) as starting materials. m/z: [M+H]
+ 599.1.
Embodiment 12: Synthesis of compounds 7-p1, 7-p2, 7-p3 and 7-p4
[0252]

[0253] Step 1: To a solution of compound 7-2 (7-2 was obtained as a mixture of stereoisomers
in the same manner as Embodiment 4 steps 1-4, by using intermediates 7-1 and 4-3 as
starting materials) (330mg, 0.3mmol) in acetonitrile (2mL) was added tert-butylamine
(2mL). The resulting mixture was stirred at room temperature for 1h, and then concentrated
under reduced pressure. The residue was purified by Flash column chromatography (acetonitrile/aqueous
solution of ammonium bicarbonate (10mmol/L)=0∼60%) to afford compound 7-3 (100mg)
as a white solid. m/z: [M+H]
+ 1063.8.
[0254] Step 2: To the solution of compound 7-3 (100 mg, 94µmol) in methanol (2mL) was added
ammonium hydroxide solution (2mL), the reaction solution was stirred in a sealed tube
at 55°C for 5h, and then the solvent was concentrated in reduced pressure. The residue
was subjected to azeotropic dehydration three times with anhydrous pyridine (2mL)
and then dissolved in pyridine (1mL) and then triethylamine (1mL) and triethylamine
trihydrofluoride (0.5mL) was added thereto under nitrogen. The resulting mixture was
stirred at 50°C for 3h. The solvent was concentrate under reduced pressure. The residue
was neutralized with ammonium hydroxide solution, and then directly purified by
prep-HPLC (separation method 5) to afford compound 7-p1 (di-ammonium salt, 1.29mg, m/z:
[M+H]
+ 741.3, HPLC-RT: 9.058min), 7-p2 (di-ammonium salt, 4.41mg, m/z: [M+H]
+ 741.3, HPLC-RT: 9.590min), 7-p3 (di-ammonium salt, 2.3mg, m/z: [M+H]
+ 741.3, HPLC-RT: 10.438min) and 7-p4 (di-ammonium salt, 24mg, m/z: [M+H]
+ 741.7, HPLC-RT: 10.929min), as white solids.
Embodiment 13: Synthesis of compounds 8-p1, 8-p2, 8-p3 and 8-p4
[0255]

[0256] Step 1: To a solution of compound 8-1 (8-1 was obtained as a mixture of stereoisomers
in the same manner as Embodiment 4 steps 1-4, by using intermediates 2-9 and 4-3 as
starting materials) (618mg, 0.61mmol) in acetonitrile (3mL) was added tert-butylamine
(3mL). The resulting mixture was stirred at room temperature for 1h, and then concentrated
under reduced pressure. The residue was purified by Flash column chromatography (acetonitrile/aqueous
solution of ammonium bicarbonate (10mmol/L) =10∼80%) to afford compounds 8-2-p1 (67
mg), 8-2-p2 (40mg), 8-2-p3 (40mg), and 8-2-p4 (87mg), as white solids. m/z: [M+H]
+ 1047.7.
[0257] Step 2: To a solution of compound 8-2-p1 (50mg, 52µmol) in methanol (1mL) was added
ammonium hydroxide solution (1mL), the reaction solution was stirred in a sealed tube
at 50°C for 4h, and then the solvent was concentrated in reduced pressure. The residue
was subjected to azeotropic dehydration three times with anhydrous pyridine (1mL)
and then dissolved in pyridine (1mL) and then triethylamine (1mL) and triethylamine
trihydrofluoride (0.5mL) was added thereto under nitrogen. The resulting mixture was
stirred at 55°C for 4h. The solvent was concentrate under reduced pressure. The residue
was neutralized with ammonium hydroxide solution, and then directly purified by
prep-HPLC (separation method 5) to afford compound 8-p1 (di-ammonium salt, 7mg, m/z: [M+H]
+ 725.5, HPLC-RT: 11.702min) as a white solid.
Synthesis of compound 8-p2:
[0258] Compound 8-p2 (di-ammonium salt, 1.98mg, m/z: [M+H]
+ 725.6, HPLC-RT: 10.602 min) was obtained as a white solid in the same manner as compound
8-p1, by using compound 8-2-p2 (40mg, 0.04mmol) as a starting material.
Synthesis of compound 8-p3:
[0259] Compound 8-p3 (di-ammonium salt, 1.1mg, m/z: [M+H]
+ 726.1, HPLC-RT: 10.556min) was obtained as a white solid in the same manner as compound
8-p1, by using compound 8-2-p3 (40mg, 0.04mmol) as a starting material.
Synthesis of compound 8-p4:
[0260] Compound 8-p4 (di-ammonium salt, 7.5mg, m/z: [M+H]
+ 725.6, HPLC-RT: 12.102min) was obtained as a white solid in the same manner as compound
8-p1, by using compound 8-2-p4 (80mg, 0.08mmol) as a starting material.
Embodiment 14: Synthesis of intermediates 9-4 and 9-5
[0261]

[0262] Step 1: The solution of isocarbostyril (25g, 172mmol), tetraacetylribose (137g, 431mmol)
and BSA (105g, 517mmol) in anhydrous acetonitrile was stirred at reflux for 1h, and
then TMSOTf (62mL, 344mmol) was added thereto after the reaction solution was cooled
to room temperature, the mixture was stirred at reflux for additional 5h, saturated
aqueous solution of sodium bicarbonate was slowly added thereto to adjusted pH to
about 7 after the reaction solution was cooled to room temperature, the aqueous layer
was extracted with ethyl acetate (150mL×3), the combined organic layers were washed
with water, the separated organic layer was dried over anhydrous sodium sulfate, filtered
and concentrated. The residue was purified by Flash column chromatography (petroleum
ether/ethyl acetate=2/1) to afford intermediate 9-1 (41.1g) as an orange oil. m/z:
[M+H]
+ 404.0.
[0263] Step 2: To a solution of intermediate 9-1 (41.1g, 102mmol) in acetonitrile (300mL)
was added aqueous solution of lithium hydroxide (510mL, 1M), and then the reaction
system was stirred at room temperature for 1.5h. An amount of white solid was precipitated,
filtered, the filter cake washed with water for 3 times, and then dried under vacuum
to afford intermediate 9-2 (18.5g) as a white solid. m/z:[M+H]
+278.0.
[0264] Step 3: Intermediate 9-2 (17.5g, 63.1mmol) was subjected to azeotropic dehydration
three times with anhydrous pyridine and then dissolved in pyridine (100mL) under nitrogen.
To the above reaction solution was added pyridine solution of DMTrCl (22.5g, 66.3mmol,
50mL) at 0°C. The resulting mixture was stirred at this temperature for 3h. The reaction
was quenched by addition of water (50mL), the aqueous layer was extracted with ethyl
acetate (100mL×2), and the combined organic layers were concentrated under reduced
pressure. The residue was purified by Flash column chromatography (petroleum ether/ethyl
acetate=1/1) to afford intermediate 9-3 (32.6 g) as an off-white solid. m/z:[M+Na]
+ 602.0.
[0265] Step 4: Intermediate 9-3 (12g, 20.7mmol) and imidazole (4.9g, 72.5mmol) were subjected
to azeotropic dehydration three times with anhydrous pyridine and then dissolved in
pyridine (50mL) under nitrogen, TBSCl (3.4g, 22.8mmol) was added thereto at 0°C. The
reaction system was stirred at room temperature for overnight, and water (50mL) was
added thereto, the aqueous layer was extracted with ethyl acetate (100mL×2), the combined
organic layers were washed with water, dried over anhydrous sodium sulfate, filtered
and concentrated, the residue was purified by Flash column chromatography (petroleum
ether/ethyl acetate=10/1∼6/1) to afford intermediates 9-4 (4.3g, off-white solid,
less polar) 9-5 (3.5g, off-white solid, more polar). 9-4: m/z: [M+Na]
+716.0;
1H NMR (400 MHz, DMSO-
d6): δ 8.28 (d,
J=8.4 Hz, 1H), 7.71 (t,
J=8.0 Hz, 2H), 7.65 (d, J=8.0 Hz, 1H), 7.53-7.45 (m, 3H), 7.36-7.32 (m, 6H), 7.26 (t,
J=8.0 Hz, 1H), 6.93 (d,
J=8.4 Hz, 4H), 6.46 (d,
J=8.0 Hz, 1H), 6.29 (d,
J=4.0 Hz, 1H), 5.14 (d,
J=6.0 Hz, 1H), 4.28-4.26 (m, 1H), 4.20-4.16 (m, 1H), 4.14-4.20(m, 1H), 4.05-4.00 (m,
1H), 3.74 (s, 6H), 3.37-3.35 (m, 1H), 0.82 (m, 9H), 0.03 (s, 3H), 0.01 (s, 3H); 9-5:
m/z:[M+Na]
+716.0;
1H NMR (400 MHz, DMSO-
d6): δ 8.27 (d,
J=8.0 Hz, 1H), 7.75-7.70 (m, 2H), 7.66-7.64 (m, 1H), 7.52 (t,
J=8.0 Hz, 1H), 7.45-7.43 (m, 2H), 7.35-7.25 (m, 7H), 6.92 (d,
J=8.4 Hz, 4H), 6.50 (d,
J=7.6 Hz, 1H), 6.27 (d,
J=3.6 Hz, 1H), 5.39 (d,
J=6.0 Hz, 1H), 4.28-4.26 (m, 1H), 4.05-4.00(m, 2H), 3.74 (s, 6H), 3.46-3.44 (m, 1H),
3.22-3.18 (m, 1H), 0.78 (m, 9H), 0.03 (s, 3H), 0.01 (s, 3H).
Embodiment 15: Synthesis of compounds 9-p1, 9-p2 and 9-p3
[0266]

[0267] Step 1: To a solution of intermediate 9-5 (3.1g, 4.47mmol) in pyridine (20mL) was
added diphenyl phosphite (3.1g, 13.4mmol) at 0°C under nitrogen, the reaction system
was stirred for 0.5h, and then triethylamine (3mL) and water (3mL) was added thereto.
The resulting mixture was stirred at room temperature for 5min and then diluted with
water (50mL), extracted with propan-2-ol/ chloroform (30mL×2), the combined organic
layers were washed with water, the separated organic layer was dried over anhydrous
sodium sulfate, filtered and concentrated. The residue was purified by Flash column
chromatography (dichloromethane/methanol/ triethylamine=100/5/1) to afford compound
9-6 (4.5g, triethylamine salt) as an off-white solid. m/z: [M-H]
-756.0.
[0268] Step 2: To a solution of compound 9-6 (3g, 3.49mmol) in a mixed solvent of dichloromethane
(28mL) and water (1mL) was added dichloromethane solution of DCA (0.6 M, 46.5mL).
The mixture was stirred at room temperature for 30min, to the the reaction solution
was added triethylsilane (28 mL) and stirred for additional 1h. When the color of
the reaction solution changes from brown to colorless, pyridine (28mL) was added thereto,
the solvent was concentrated under reduced pressure to afford compound 9-7 (3g, pyridinium
salt, curd product). m/z: [M+H]
+ 445.0.
[0269] Step 3&4: Compound 9-7 (3g, crude) was dissolved in anhydrous acetonitrile (15mL)
and then concentrated under reduced pressure, repeated three times. The residue was
dissolved in acetonitrile (50mL), and 4A molecular sieve (1g) was added thereto. 3'-TBDMS-ibu-rG
Phosphoramidite (
CAS No.: 1445905-51-0, 3.4g, 3.49mmol) was dissolved in anhydrous acetonitrile (15 mL) and concentrated
under reduced pressure, repeated three times. The residue was dissolved in acetonitrile
(20mL), and 4A molecular sieve (2g) was added thereto. To a solution of 9-7 in acetonitrile
was slowly added the acetonitrile solution of 3'-TBDMS-ibu-rG Phosphoramidite at 0°C,
the resulting mixture was stirred at room temperature for 0.5h, and then DDTT (697
mg, 3.42 mmol) was added thereto and stirred at room temperature for additional 3h.
The molecular sieve was removed by filtration. To the mixtue was added water (1mL)
and then slowly added DCA (3.6g, 27.9mmol) dropwise, the resulting mixture was stirred
at room temperature for 2h, triethylsilane (28mL) was added thereto and stirred for
additional 1h, pyridine (28mL) was added thereto, and the reaction solution was concentrated
under reduced pressure. The residue was purified by Flash column chromatography (acetonitrile/
aqueous solution of ammonium bicarbonate (10mmol/L)=50%) to afford compound 9-10 (680mg)
as a white solid. m/z: [M+H]
+ 1053.9.
[0270] Step 5: To a solution of compound 9-10 (200mg, 0.19mmol) in anhydrous pyridine (8mL)
was added DMOPC (0.7g, 3.8mmol) for one charge, the mixture was stirred at room temperature
for 0.5h, to the above reaction solution was added 3
H-1,2-benzodithiol-3-one (38mg, 0.23mmol), and then stirred at room temperature for
0.5h, the reaction was quenched by addition of aqueous solution of sodium bicarbonate
(2.7%). The mixture was extracted with ethyl acetate (50mL×2), and the combined organic
layers were washed with brine, dried over anhydrous sodium sulfate, filtered and concentrated
under reduced pressure. The residue was purified by
prep-HPLC (separation method 5) to afford compound 9-11-p1 (13.5mg, LCMS-RT (Thermo):
2.023min), 9-11-p2 (30.2mg, LCMS-RT (Thermo): 2.157min), and 9-11-p3 (38.0mg, LCMS-RT
(Thermo): 2.300min), as white solids. m/z: [M+H]
+ 1067.9.
[0271] Step 6: Compound 9-11-p1 (10mg, 0.094mmol) was dissolved in methylamine ethanol solution
(1mL, 30%) and stirred at room temperature for 1h, and then concentrated under reduced
pressure, the residue was dissolved in pyridine (0.5mL), triethylamine (0.5mL) and
triethylamine trihydrofluoride (0.25mL) was added thereto, the reaction system was
stirred at 50°C for 5h and then concentrated under reduced pressure. The residue was
diluted with methanol, and then ammonium hydroxide solution was slowly dropped to
adjusted pH to 8∼9. The resulting mixture was purified by
prep-HPLC (separation method 5) to afford compound 9-p1 (4.07mg, HPLC-RT: 11.311min;m/z:
[M+H]
+ 716.8;
1H NMR (400 MHz, DMSO-
d6): δ 8.26 (d,
J=6.0 Hz, 1H), 8.19 (s, 1H), 7.73 (t,
J=7.6 Hz, 1H), 7.68 (d,
J=8.0 Hz, 1H), 7.53 (t,
J=8.0 Hz, 1H), 7.42 (d,
J=7.6 Hz, 1H), 6.69 (d,
J=7.6 Hz, 1H), 6.51-6.47 (m, 2H), 6.22 (d,
J=4.0 Hz, 1H), 5.90-5.82 (m, 2H), 5.52-5.51 (m, 1H), 5.08-5.06 (m, 1H), 4.95-4.90 (m,
1H), 4.88-4.86 (m, 1H), 4.65-4.61 (m, 1H), 4.57-4.55 (m, 1H), 4.40-4.36 (m, 2H), 4.25-4.19
(m, 2H), 4.10-3.99 (m, 2H), 3.94-3.87 (m, 2H);
31P NMR (162 MHz, DMSO-
d6): δ 56.49, 54.10.) a as white solid.
Synthesis of compounds 9-p2:
[0272] Compound 9-p2 (4.45mg, HPLC-RT: 11.389min; m/z: [M+H]
+ 706.8;
1H NMR (400 MHz, DMSO-
d6): δ 8.26 (d,
J=8.0 Hz, 1H), 8.02 (s, 1H), 7.74 (t,
J=7.2 Hz, 1H), 7.67 (d,
J=7.6 Hz, 1H), 7.53 (t,
J=7.6 Hz, 1H), 7.37 (d,
J=7.6 Hz, 1H), 6.91-6.34 (m, 4H), 5.87-5.85 (m, 1H), 5.37-5.19 (m, 2H), 4.38-4.37 (m,
1H), 4.21-4.19 (m, 1H), 4.11-4.05 (m, 2H), 3.74-3.70 (m, 1H), 3.01-2.99 (m, 8H);
31P NMR (162 MHz, DMSO-
d6): δ 59.18, 56.65, 54.32, 47.74.) was obtained as a white solid in the same manner
as compound 9-p1, by using compound 9-11-p2 as a starting material and purified by
prep-HPLC (separation method 5).
Synthesis of compounds 9-p3:
[0273] Compound 9-p3 (7.0 mg, HPLC-RT: 10.912min; m/z: [M+H]
+ 716.8;
1H NMR (400 MHz, DMSO-
d6): δ 8.26 (d,
J=8.0 Hz, 1H), 7.97 (s, 1H), 7.73 (t,
J=7.6 Hz, 1H), 7.65 (d,
J=7.6 Hz, 1H), 7.53 (t,
J=7.6 Hz, 1H), 7.44 (d,
J=7.6 Hz, 1H), 6.62 (d,
J=8.0 Hz, 1H), 6.56-6.52 (m, 1H), 6.33 (d,
J=6.8 Hz, 1H), 5.86 (d,
J=8.4 Hz, 1H), 5.27-5.23 (m, 1H), 5.06-5.04 (m, 2H), 4.46-4.40 (m, 2H), 4.22-4.18 (m,
1H), 4.12-4.08 (m, 2H), 3.96-3.92 (m, 2H), 3.78-3.75 (m, 1H), 3.01-2.95 (m, 4H);
31P NMR (162 MHz, DMSO-
d6): δ 57.77, 50.27.) was obtained as a white solid in the same manner as compound 9-p1,
by using compound 9-11-p3 as a starting material, and purified by
prep-HPLC (separation method 5).
Embodiment 16: Synthesis of compounds 10-p1, 10-p2 and 10-p3
Synthesis of intermediates 10-4 and 10-5:
[0274]

[0275] Intermediates 10-4 (2.0g, more polar) and 10-5 (3.4g, less polar) were obtained as
white solids in the same manner as compounds 9-4 and 9-5, by using quinazolin-4(3
H)-one as a starting material. m/z: [M+H]
+ 695.0; 10-4:
1H NMR (400 MHz, DMSO-
d6): δ 8.50 (s, 1H), 8.16 (d,
J=8.0 Hz, 2H), 7.86-7.82 (m, 1H), 7.67 (d,
J=8.0 Hz, 2H), 7.58-7.54 (m, 1H), 7.40 (d,
J=8.0 Hz, 2H), 7.31-7.22 (m, 7H), 6.89-6.87 (m, 4H), 6.09 (d,
J=4.0 Hz, 1H), 5.10 (d,
J=8.0 Hz, 1H), 4.38-4.36 (m, 1H), 4.13-4.09 (m, 2H), 3.72 (s, 6H), 0.81 (s, 9H), 0.01-0.00
(s, 6H); 10-5:
1H NMR (400 MHz, DMSO-
d6): δ 8.53 (s, 1H), 8.15 (d,
J=8.0 Hz, 1H), 7.86-7.82 (m, 1H), 7.67 (d,
J=8.0 Hz, 1H), 7.58-7.54 (m, 1H), 7.41-7.39 (m,2H), 7.30-7.19 (m, 7H), 6.85 (d,
J=8.0 Hz,4H), 6.06-6.05 (m, 1H), 5.36 (d,
J=8.0 Hz, 1H), 4.31-4.26 (m, 2H), 4.02-4.00 (m, 1H), 3.71 (s, 6H), 0.79 (s, 9H), 0.02-0.00
(s, 6H).

[0276] Synthesis of compounds 10-11-p1, 10-11-p2 and 10-11-p3: compounds 10-11-p1 (7.0mg,
LCMS-RT (Thermo): 2.020min), 10-11-p2 (26mg, LCMS-RT (Thermo): 2.170min) and 10-11-p3
(19mg, LCMS-RT (Thermo):2.350min) were obtained as white solids in the same manner
as Embodiment 15 steps 1∼5, by using compound 10-5 as a starting material, and purified
by
prep-HPLC (separation method 6). m/z: [M+H]
+ 1068.9.
Synthesis of compound 10-p1:
[0277] To compound 10-11-p1 (7.0mg, 0.006mmol) was added methylamine ethanol solution (1
mL, 30%), the reaction system was stirred at room temperature for 1h and then concentrated
under reduced pressure, the residue was dissolved in anhydrous pyridine (0.5mL), triethylamine
(0.5mL) and triethylamine trihydrofluoride (0.25mL) was added thereto under nitrogen,
the reaction solution was stirred at 50°C for 1h. The solvent was concentrated under
reduced pressure, the residue was diluted with methanol, and then ammonium hydroxide
solution was slowly dropped to adjusted pH to 8∼9. The mixture was purified by
prep-HPLC (separation method 4) to afford compound 10-p1 (0.73mg, HPLC-RT: 10.671min,
m/z: [M+H]
+717.8) as a white solid.
Synthesis of compound 10-p2:
[0278] Compound 10-p2 (7.10mg, HPLC-RT: 10.826min; m/z: [M+H]
+717.8;
1H NMR (400 MHz, DMSO-
d6): δ 8.41 (s, 1H), 8.25-8.20 (m, 1H), 8.03 (s, 1H), 7.89-7.85 (m, 1H), 7.72-6.69 (m,
1H), 7.61-7.57 (m, 1H), 7.61-7.57 (m, 1H), 6.52-6.48 (m, 2H), 6.07-6.00 (m, 1H), 5.91-5.79
(m, 1H), 0.56 (s, 1H), 5.34-5.30 (m, 1H), 5.20-5.19 (m, 1H), 5.09-5.01 (m, 1H), 4.70-4.64
(m, 1H), 4.36-4.33 (m, 1H), 4.27-4.18 (m, 1H), 4.14-4.05 (m, 2H), 3.95-3.70 (m, 3H),
1.24 (s, 2H).) was obtained as a white solid in the same manner as compound 10-p1,
by using compound 10-11-p2 as a starting material, and purified by
prep-HPLC (separation method 4).
Synthesis of compound 10-p3:
[0279] Compound 10-p3 (5.46mg, HPLC-RT:10.925min; m/z: [M+H]
+717.8;
1H NMR (400 MHz, DMSO-
d6): δ 8.44 (s, 1H), 8.22-8.20 (m, 1H), 7.99 (s, 1H), 7.89-7.85 (m, 1H), 7.68 (d,
J=8.0 Hz,1H), 7.61-7.57 (m, 1H), 6.56 (s, 2H), 6.09 (d,
J=4.0 Hz,2H), 5.83 (d,
J=8.0 Hz,1 H), 5.34-5.32 (m, 1H), 5.26 (s, 1H), 5.08 (m, 1H), 4.71-4.68 (m, 1H), 4.46-4.45
(m, 1H), 4.25 (m, 1H), 4.14-3.95 (m, 5H), 3.79-3.76 (m,1H), 1.24 (s, 2H).) was obtained
as a white solid in the same manner as compound 10-p1, by using compound 10-11-p3
as a starting material, and purified by
prep-HPLC (separation method 4).
Embodiment 17: Synthesis of compounds 11-p1, 11-p2, 11-p3 and 11-p4
Synthesis of intermediates 11-4 and 11-5:
[0280]

[0281] Synthesis of compounds 11-4 and 11-5: compounds 11-4 (650mg, more polar) and 11-5
(610mg, less polar) were obtained as white solids in the same manner as compounds
9-4 and 9-5, by using pyrido[2,3-
d]pyrimidin-4-one as a starting material. m/z: [M+H]
+ 696.0; 11-4:
1H NMR (400 MHz, DMSO-
d6): δ 8.97 (d,
J=4.0 Hz, 1H), 8.74 (s, 1H), 8.56 (d,
J=8.0 Hz, 1H), 7.60-7.57 (m, 1H), 7.42 (d,
J=8.0 Hz, 2H), 7.31-7.20 (m, 1H), 6.88 (d,
J=12.0 Hz, 4H), 6.02 (s, 1H), 5.40 (d,
J=4.0 Hz, 1H),4.30-4.29 (m, 2H), 4.01-3.98 (m, 1H), 3.71 (s, 6H), 3.40 - 3.37 (m, 1H),
3.21-3.18 (m, 1H), 0.74 (s, 9H),0.00 (s, 6H); 11-5:
1H NMR (400 MHz, DMSO-
d6): δ 8.94 (d,
J=4.0 Hz,1H), 8.67 (s, 1H), 8.55 (d,
J=8.0 Hz,1H), 7.56-7.53 (m, 1H), 7.41(d,
J=8.0 Hz, 2H), 7.29-7.25 (m, 1H), 6.86 (d,
J=8.0 Hz, 4H), 6.00 (s, 1H), 5.13 (d,
J=4.0 Hz, 1H), 4.13-4.08 (m, 2H), 4.00-3.98 (m, 1H), 3.39 (s, 6H), 3.36-3.32 (m, 1H),
3.27-3.25 (m, 1H), 0.79 (s, 9H), 0.00 (s, 6H).

[0282] Synthesis of compounds 11-11-p1, 11-11-p2, 11-11-p3 and 11-11-p4: compounds 11-11-p1
(27mg), 11-11-p2 (15mg), 11-11-p3 (19mg) and 11-11-p4 (36mg) were obtained as white
solids in the same manner as Embodiment 15 steps 1∼5, by using compound 11-5 as a
starting material, and purified by
prep-HPLC (separation method 6).
Synthesis of compound 11-p1:
[0283] To compound 11-11-p1 (20mg, 0.019mmol) was added methylamine ethanol solution (2
mL, 30%), the reaction system was stirred at room temperature for 10h and then concentrated
under reduced pressure, the residue was dissolved in anhydrous pyridine (0.5mL), and
triethylamine (0.5mL) and triethylamine trihydrofluoride (0.25mL) were added thereto
under nitrogen, the reaction solution was stirred at 50°C for 1h. The solvent was
concentrated under reduced pressure. The residue was diluted with methanol, and then
ammonium hydroxide solution was slowly added to dropped pH to 8∼9. The mixture was
purified by
prep-HPLC (separation method 7) to afford compound 11-p1 (2.45mg, HPLC-RT: 8.963min, m/z:
[M+H]
+718.8) as a white solid.
Synthesis of compound 11-p2:
[0284] Compound 11-p2 (3.07mg, HPLC-RT: 8.527min, m/z: [M+H]
+718.7) was obtained as a white solid in the same manner as compound 11-p1, by using
compound 11-11-p2 as a starting material, and purified by
prep-HPLC (separation method 5).
Synthesis of compound 11-p3:
[0285] Compound 11-p3 (1.28mg, HPLC-RT: 9.103min, m/z: [M+H]
+719.1) was obtained as a white solid in the same manner as compound 11-p1, by using
compound 11-11-p3 as a starting material, and purified by
prep-HPLC (separation method 5).
Synthesis of compound 11-p4:
[0286] Compound 11-p4 (3.03mg, HPLC-RT: 9.403min, m/z: [M+H]
+718.6) was obtained as a white solid in the same manner as compound 11-p1, by using
compound 11-11-p4 as a starting material, and purified by
prep-HPLC (separation method 5).
Embodiment 18: Synthesis of intermediate 12-1
[0287]

[0288] Step 1: 2,6-dichloronicotinonitrile (25g, 145mmol) and ammonium hydroxide solution
(250mL) was charged into a sealed tube. The reaction system was stirred at 120°C for
48h and then cooled to 10°C, the solid was filtered and washed with cold water, the
the filter cake was dried under vacuum to afford 2,6-diaminonicotinonitrile (12.5g)
as a light yellow solid. m/z: [M+H]+135.0.
[0289] Step 2: To a solution of 2,6-diaminonicotinonitrile (12.5g, 93.2mmol) in acetic acid
(120mL) was slowly added concentrated sulfuric acid (3mL), the reaction system was
stirred at reflux for 9h, and then cooled to room temperature and concentrated under
reduced pressure. The residue was triturated with ammonium hydroxide solution, the
solid was filtered, the filter cake washed with cold water, and dried under vacuum
to afford 7-aminopyrido[2,3-
d] pyrimidin-4(3
H)-one (15.1g) as a light yellow solid. m/z: [M+H]
+ 163.0.
[0290] Step 3: 7-aminopyrido[2,3-
d]pyrimidin-4(3
H)-one (15.1g, 93.1mmol) was dissolved in pyridine (1.5L), isobutyryl chloride (29.7g,
279mmol) was slowly added thereto, the reaction system was stirred at room temperature
for 18h and then water (1.5L) was added thereto, the reaction solution was extracted
with chloroform /propan-2-ol (1.5L), the organic layer was concentrated under reduced
pressure. The residue was purified by Flash column chromatography (0-6.2%methanol/
dichloromethane) to afford compound 12-1 (11.9g) as a white solid. m/z: [M+H]+233.1.
Embodiment 19: Synthesis of compounds 12-p1, 12-p2, 12-p3 and 12-p4
Synthesis of intermediates 12-4 and 12-5:
[0291]

[0292] Intermediates 12-4 (1.73g, more polar) and 12-5 (1.1g, less polar) were obtained
as white solids in the same manner as compounds 9-4 and 9-5, by using compound 12-1
as a starting material. m/z: [M+H]+781.0; 12-4:
1H NMR (400 MHz, DMSO-
d6): δ 11.15 (s, 1H), 8.70 (s, 1H), 8.52 (d,
J=8.8 Hz, 1H), 8.33 (d,
J=8.8 Hz, 1H), 7.43 (d,
J=7.6 Hz, 2H), 7.32 (d,
J=7.6 Hz, 2H), 7.29 (d,
J=8.4 Hz, 4H), 6.89 (d,
J=8.4 Hz, 4H), 6.03 (d,
J=2.8 Hz, 1H), 5.40 (d,
J=6.0 Hz, 1H), 4.30 (t,
J=3.2 Hz, 1H), 4.05-4.02 (m, 2H), 3.73 (s, 6H), 3.40-3.36 (m, 1H), 3.25-3.22 (m, 1H),
2.83-2.79 (m, 1H), 1.12 (s, 3H), 1.11 (s, 3H), 0.77 (s, 9H), 0.03 (s, 3H),-0.04 (s,
3H); 12-5:
1H NMR (400 MHz, DMSO-
d6): δ 11.13 (s, 1H), 8.67 (s, 1H), 8.54 (d,
J=8.8 Hz, 1H), 8.31 (d,
J=8.8 Hz, 1H), 7.45 (d,
J=7.6 Hz, 2H), 7.33 (d,
J=7.6 Hz, 2H), 7.31 (d,
J=8.4 Hz, 4H), 6.90 (d,
J=8.4 Hz, 4H), 6.04 (d,
J=2.8 Hz, 1H), 5.16 (d,
J=6.0 Hz, 1H), 4.40 (t,
J=3.2 Hz, 1H), 4.17-4.12 (m, 2H), 3.74 (s, 6H), 3.43-3.40 (m, 1H), 3.30-3.27 (m, 1H),
2.82-2.79 (m, 1H), 1.13 (s, 3H), 1.11 (s, 3H), 0.85 (s, 9H), 0.05 (s, 6H).

[0293] Synthesis of compounds 12-11-p1, 12-11-p2, 12-11-p3 and 12-11-p4: compounds 12-11-pl
(25mg), 12-11-p2 (7mg), 12-11-p3 (40mg) and 12-11-p4 (32mg) were obtained as white
solids in the same manner as Embodiment 15 steps 1∼5, by using compound 12-5 as a
starting material, and purified by
prep-HPLC (separation method 6).
Synthesis of compound 12-p1:
[0294] To compound 12-11-p1 (25mg, 19 µmol) was added methylamine ethanol solution (2mL,
30%), the reaction system was stirred at room temperature for 3h and then concentrated
under reduced pressure, the residue was dissolved in anhydrous pyridine (0.5mL), triethylamine
(0.5mL) and triethylamine trihydrofluoride (0.25mL) was added thereto under nitrogen,
the reaction solution was stirred at 50°C for 1h. The solvent was concentrated under
reduced pressure. The residue was diluted with methanol, and then ammonium hydroxide
solution was slowly dropped to adjusted pH to 8∼9. The mixture was purified by
prep-HPLC (separation method 4) to afford compound 12-p1 (0.9mg, HPLC-RT: 8.891min, m/z: [M+H]+733.7)
as a white solid.
Synthesis of compound 12-p2:
[0295] Compound 12-p2 (0.5mg, HPLC-RT: 8.571min, m/z: [M+H]+733.7) was obtained as a white
solid in the same manner as compound 12-p1, by using compound 12-11-p2 as a starting
material, and purified by
prep-HPLC (separation method 4).
Synthesis of compound 12-p3:
[0296] Compound 12-p3 (1.5mg, HPLC-RT: 10.020min, m/z: [M+H]+733.8) was obtained as a white
solid in the same manner as compound 12-p1, by using compound 12-11-p3 as a starting
material, and purified by
prep-HPLC (separation method 4).
Synthesis of compound 12-p4:
[0297] Compound 12-p4 (3.07mg, HPLC-RT: 9.468min, m/z: [M+H]+733.8) was obtained as a white
solid in the same manner as compound 12-p1, by using compound 12-11-p4 as a starting
material, and purified by
prep-HPLC (separation method 4)
Embodiment 20: Synthesis of intermediates 13-4 and 13-5
[0298]

[0299] Synthesis of imidazo[1,2-c]pyrimidin-5(6
H)-one: The suspension of cytosine (18g, 0.16mol), aqueous solution of chloroacetaldehyde
(63.6g, 0.32mol, 40%) and sodium acetate (32.8g, 0.40mol) in water (180mL) was stirred
at 80°C for 3h, and then the reaction system was cooled to 10°C, the solid was filtered
and washed with cold water, the filter cake was dried under vacuum to afford imidazo[1,2-
c]pyrimidin-5(6
H)-one (17.8g) as a brown solid. m/z: [M+H]+ 136.0.
[0300] Step 1: To a suspension of imidazo[1,2-
c]pyrimidin-5(6
H)-one (17.8g, 0.13mmol), tetraacetylribose (46.1g, 0.14mmol) and BSA (39.7g, 0.19mmol)
in acetonitrile (200mL) was added TMSOTf (43.3g, 0.19mol) dropwise at 0°C, the reaction
system was stirred at 60°C for 3h and then concentrated under reduced pressure to
afford compound 13-1 (100g, crude product) as a brown oil. m/z: [M+H]
+ 394.0.
[0301] Step 2: Compound 13-1 (45g, crude) was dissolved in methanol (100mL), ammonia in
methanol (81.7mL, 7M) was slowly added thereto, the reaction system was stirred at
room temperature for overnight, the solid was filtered and washed with methanol, the
filter cake was dried under vacuum to afford compound 13-2 (8g) as a brown solid.
m/z: [M+H]
+ 268.0.
[0302] Step 3: Compound 13-2 (12.5g, 5.76mmol) was subjected to azeotropic dehydration three
times with anhydrous pyridine (20mL) and then dissolved in pyridine (100mL). To the
above reaction solution was added DMTrCl (19.52g, 5.76mmol) in small portions at 0∼5°C
under nitrogen. The reaction system was stirred at room temperature for overnight,
the solvent was concentrated under reduced pressure, and the residue was diluted with
chloroform (100mL). The organic layer washed with saturated aqueous solution of sodium
bicarbonate (30mLx2), the organic layer was separated and dried over anhydrous sodium
sulfate, filtered and concentrated. The residue was purified by Flash column chromatography
(methanol/ dichloromethane=0-3%) to afford compound 13-3 (16.65g) as a brown solid.
m/z:[M+Na]
+ 570.0.
[0303] Step 4: Compound 13-3 (5.38g, 9.44mmol) was subjected to azeotropic dehydration three
times with anhydrous pyridine (20mL) and then dissolved in pyridine (60mL). To the
mixture was added TBSCl (2.13g, 14.2mmol) and imidazole (2.25g, 33.1mmol) at 0°C under
nitrogen. The reaction system was stirred at room temperature for overnight, the solvent
was concentrated under reduced pressure, and the residue was diluted with chloroform
(100mL). The organic layer washed with saturated aqueous solution of sodium bicarbonate
(30mLx2), the separated organic layer was dried over anhydrous sodium sulfate, filtered
and the filtrate was concentrated under reduced pressure. The residue was purified
by Flash column chromatography (0-3% methanol/ dichloromethane) to afford compounds
13-4 (1.32g, less polar) and 13-5 (3.68g, more polar), as white foam solids. m/z:
[M+H]+ 684.0; 13-4:
1H NMR (400 MHz, DMSO-
d6): δ 7.88 (d,
J=5.6 Hz, 1H), 7.82 (s, 1H), 7.76 (s, 1H), 7.40-7.26 (m, 9H), 6.92-6.90 (m, 4H), 6.68
(d,
J=6.0 Hz, 1H), 5.90 (d,
J=9.2 Hz, 1H), 5.33 (d,
J=4.8 Hz, 1H), 4.50 (t,
J=4.4 Hz, 1H), 4.15-4.14 (m, 2H), 3.74 (s, 6H), 3.28-3.26 (m, 2H), 0.77 (s, 9H), 0.02
(s, 3H), -0.12 (s, 3H). 13-5:
1H NMR (400 MHz, DMSO-
d6): δ 7.92 (d,
J=6.0 Hz, 1H), 7.78 (s, 2H), 7.36-7.21 (m, 9H), 6.88-6.86 (m, 4H), 6.68 (d,
J=8.8 Hz, 1H), 5.81 (d,
J=4.8 Hz, 1H), 5.63 (d,
J=6.0 Hz, 1H), 4.40-4.35 (m, 2H), 4.27 (t,
J=4.8 Hz, 1H),4.06-4.04 (m, 1H), 3.74 (s, 6H), 3.20-3.16 (m, 1H), 0.80 (s, 9H), 0.06
(s, 3H), -0.01 (s, 3H).
Embodiment 21: Synthesis of compounds 13-p1, 13-p2, 13-p3 and 13-p4
[0304]

[0305] Synthesis of compounds 13-11-p1, 13-11-p2, 13-11-p3 and 13-11-p4: compounds 13-11-p1
(55mg, LCMS-RT (Thermo):1.874, 1.944min), 13-11-p2 (25mg, LCMS-RT (Thermo):2.030min),
and 13-11-p3 (11mg, LCMS-RT (Thermo):2.170min) were obtained as white solids in the
same manner as Embodiment 15 steps 1∼5, by using compound 13-5 as a starting material,
and purified by Flash column chromatography (acetonitrile/ aqueous solution of ammonium
bicarbonate (10mmol/L) =40%). m/z: [M+H]+ 1057.9.
Synthesis of compounds 13-p1 and 13-p2:
[0306] Compound 13-11-p1 (43mg, 0.04mmol) was dissolved in methylamine ethanol solution
(3 mL, 30%) and stirred at room temperature for 3h, and then concentrated under reduced
pressure, the residue was dissolved in anhydrous pyridine (0.5mL), triethylamine (0.5mL)
and triethylamine trihydrofluoride (0.25mL) was added thereto under nitrogen, the
reaction solution was stirred at 50°C for 3h and then concentrated under reduced pressure.
The residue was diluted with methanol and ammonium hydroxide solution was slowly dropped
to adjusted pH to 8∼9. The mixture was purified by
prep-HPLC (separation method 5) to afford compounds 13-p1 (2.28mg, HPLC-RT: 6.048min, m/z:
[M+H]+ 706.8) and 13-p2 (0.68mg, HPLC-RT: 7.781min, m/z: [M+H]+ 706.8), as white solids.
Synthesis of compound 13-p3:
[0307] Compound 13-p3 (3.82mg, HPLC-RT: 6.445min, m/z: [M+H]+ 706.8) was obtained as a white
solid in the same manner as compounds 13-p1/13-p2, by using compound 13-11-p2 as a
starting material, and purified by
prep-HPLC (separation method 4).
Synthesis of compound 13-p4:
[0308] Compound 13-p4 (4.03mg, HPLC-RT: 10.395min, m/z: [M+H]+ 706.8) was obtained as a
white solid in the same manner as compounds 13-p1/13-p2, by using compound 13-11-p3
as a starting material, and purified by
prep-HPLC (separation method 5).
Embodiment 22: Synthesis of compounds 14-p1, 14-p2, 14-p3 and 14-p4
Synthesis of intermediates 14-4 and 14-5:
[0309]

[0310] Intermediates 14-4 (1.3g, more polar) and 14-5 (0.56g, less polar) were obtained
as white solids in the same manner as compounds 13-4 and 13-5, by using imidazo[5,1-
f][1,2,4]triazin-4(3
H)-one (
CAS No.: 865444-76-4) as a starting material. m/z: [M+H]+ 685.0.

[0311] Synthesis of compounds 14-11-p1, 14-11-p2, 14-11-p3 and 14-11-p4: compounds 14-11-p1
(5mg, LCMS-RT (Thermo): 1.850min), 14-11-p2 (17mg, LCMS-RT (Thermo): 1.880min), 14-11-p3
(22mg, LCMS-RT (Thermo): 2.020min) and 14-11-p4 (18mg, LCMS-RT (Thermo): 2.140min)
were obtained as white solids in the same manner as Embodiment 15 steps 1∼5, by using
compound 14-5 as a starting material, and purified by
prep-HPLC (separation method 7). m/z: [M+H]
+ 1059.0.
Synthesis of compound 14-p1:
[0312] Compound 14-11-p1 (5.0mg, 0.05mmol) was dissolved in methylamine ethanol solution
(3.0mL, 30%), the mixture was stirred at room temperature for 3h and then concentrated
under reduced pressure, the residue was dissolved in anhydrous pyridine (0.5mL), triethylamine
(0.5mL) and triethylamine trihydrofluoride (0.3mL) was added thereto under nitrogen,
the reaction solution was stirred at 50°C for 2h. The solvent was concentrated under
reduced pressure. The residue was diluted with methanol and then ammonium hydroxide
solution was slowly dropped to adjusted pH to 8∼9. The mixture was purified by
prep-HPLC (separation method 5) to afford compound 14-p1 (3.65mg, HPLC-RT: 1.914min, m/z: [M+H]+707.5)
as a white solid.
Synthesis of compound 14-p2:
[0313] Compound 14-p2 (17.5mg, HPLC-RT: 3.123min, m/z: [M+H]+707.5) was obtained as a white
solid in the same manner as compound 14-p1, by using compound 14-11-p2 as a starting
material, and purified by
prep-HPLC (separation method 4).
Synthesis of compound 14-p3:
[0314] Compound 14-p3 (1.04mg, HPLC-RT: 3.193min, m/z: [M+H]+707.5) was obtained as a white
solid in the same manner as compound 14-p1, by using compound 14-11-p3 as a starting
material, and purified by
prep-HPLC (separation method 4).
Synthesis of compound 14-p4:
[0315] Compound 14-p4 (1.04mg, HPLC-RT: 36.036min, m/z: [M+H]+707.5) was obtained as a white
solid in the same manner as compound 14-p1, by using compound 14-11-p4 as a starting
material, and purified by
prep-HPLC (separation method 4).
Embodiments of bioassays
Embodiment 1: Type 1 IFN activation assay
[0316] Plated THP-1 dual cells (Invivogen) as 100000 cells/well in a 96 well-plate, and
then phorbol 12-myristate 13-acetate (PMA) was added and the final concentration of
PMA was 30ng/mL. After 24h incubation, cells were washed with fresh medium for twice,
and 3-folds dilution compounds were added to appropriate wells. The compounds were
diluted by PB buffer (50mM HEPES, 100mM KCl, 3mM MgCl
2, 0.1mM DTT, 85mM sucrose, 1mM ATP, 0.1mM GTP, 0.2% bovine serum albumin and 5µg/ml
digitonin) and the top dose was 10µM, minimum dose was 0.0015µM. The cells were incubated
for 30min, and then washed with fresh medium for twice again. Add fresh medium, and
the cells were incubated for additional 24h. After incubation, 10ul supernant and
50µL QUANTI-Luc (Invivogen) was add to a 96 well-plate, fluorescence was read with
TECAN. The express level of IFN was proportional to the fluorescence intensity. Drew
the curve with Graphpad Prism and analyse EC
50 of the compounds.
Compound No. |
EC50 (µM) |
Compound No. |
EC50 (µM) |
1-p1 |
>10 |
8-p3 |
99.53 |
1-p2 |
>10 |
8-p4 |
38.80 |
1-p3 |
>10 |
9-p1 |
>100 |
1-p4 |
25.615 |
9-p2 |
>100 |
2-p1 |
45.755 |
9-p3 |
4.865 |
2-p2 |
>100 |
10-p1 |
70.69 |
2-p3 |
97.411 |
10-p2 |
118.8 |
2-p4 |
2.077 |
10-p3 |
0.494 |
4-p1 |
6.834 |
11-p1 |
60.81 |
4-p2 |
0.625 |
11-p2 |
9.805 |
4-p3 |
0.417 |
11-p3 |
17.50 |
5-p1 |
>10 |
11-p4 |
0.421 |
5-p2 |
51.981 |
12-p1 |
>100 |
5-p3 |
58.238 |
12-p2 |
19.01 |
5-p4 |
3.223 |
12-p3 |
8.628 |
6-p1 |
2.273 |
12-p4 |
0.565 |
6-p2 |
1.063 |
13-p1 |
188.1 |
6-p3 |
0.429 |
13-p2 |
351.6 |
6-p4 |
0.311 |
13-p3 |
176.0 |
7-p1 |
3.464 |
13-p4 |
4.991 |
7-p2 |
40.62 |
14-p1 |
>100 |
7-p3 |
0.275 |
14-p2 |
>100 |
7-p4 |
2.307 |
14-p3 |
40.35 |
8-p1 |
>100 |
14-p4 |
21.52 |
8-p2 |
>100 |
Ref.1 |
2.942 |
Embodiment 2: STING IFNβ secretion assay
[0317] Plated 40uL THP-1 cells (ATCC) as 16000 cells/well in a 96 well-plate (Corning, 3596).
3-folds dilution compounds were added to appropriate wells. The compounds were diluted
with buffer (RPMI1640+2mM L-glutamine + 1x non-essential amino acids + 1mM sodium
pyruvate + 0.5% Fetal bovine serum), and the top dose of the compound was 100µM, minimum
dose was 1.23µM. After 5h incubation, 2µL supernant was added to 384-well plate (Greiner,
Cat: 784075) and the secretion of IFN-β was detected with AllphaLISA IFN-β kit(PerkinElmer,
Cat:AL577C). 1x buffer was prepared to dilute the receptor and dornor, and then 4µL
20µg/mL Anti-pIFNP AlphaLISA receptor was added to every well. After 30mins incubation
at room temperature, 4µL 2nM biotinylated Anti-pIFNP antibody was added to a 384 well-plate,
incubated overnight at 4°C. 10µL 40µg/mL Streptavidin (SA) dornor which was diluted
with 1xbuffer was added, and then incubated at room temperature for 30min. Fluorescence
was read with TECAN. The expressed level of IFN-β was proportional to the fluorescence
intensity. Drew the curve with Graphpad Prism and analysed EC
50 of the compounds.
Compound No. |
EC50 (µM) |
2-p4 |
47.64 |
4-p2 |
36.22 |
4-p3 |
94.39 |
6-p2 |
58.09 |
6-p3 |
9.84 |
6-p4 |
9.40 |
Embodiment 3: Efficacy study in a CT-26 colon tumor xenograft model in mice
[0318] Cell Culture: The CT26 colon tumor cells (ATCC) were maintained in vitro as a monolayer
culture in RPMI1640 medium supplemented with 10% fetal bovine serum at 37°C in an
atmosphere of 5% CO2 in air. The tumor cells were routinely passaged twice per week
by trypsin-EDTA treatment. The cells growing in an exponential growth phase were harvested
and counted for tumor inoculation.
[0319] Animals: BALB/c nude mice, 6-8weeks, 19-22g, animal supplier: Beijing Vital River
Laboratory Animal Technology Co., Ltd.
[0320] 4 groups were set up as follows:
Group |
No. |
Treatment |
Dose |
Dosing Route |
Actual Schedule |
1 |
8 |
Vehicle |
- |
i.t. |
Q3D x 3 doses |
2 |
8 |
Ref.1 |
2 mg/kg |
i.t. |
Q3D x 3 doses |
3 |
8 |
Compound 6-p3 |
1 mg/kg |
i.t. |
Q3D x 3 doses |
4 |
8 |
Compound 6-p3 |
2 mg/kg |
i.t. |
Q3D x 3 doses |
Note: i.t.: intra-tumor injection, Q3D: once every 3 days |
[0321] Experiment method: Each mouse was inoculated subcutaneously with CT-26 tumor cells
(1×10
5) in 0.1mL over right flank region. The growth of tumor was observed regularly, when
the tumor volume reached 150mm
3, mice were randomized based on tumor volume and body weight, and treated with schedule.
The weight and tumor size of mice were measured 2-3 times a week during the whole
experiment.
[0322] Tumor size formula: Tumor volumes (mm
3)=0.5×(long diameters of the tumorxshort diameters of the tumor
2).
[0323] The tumor growth curves of different 4 groups are shown in Figures 1. The result
indicated that compared with the positive control Ref.1, the compound of the disclosure
can show better efficacy on the subcutaneous CT-26 colon tumor model in mice.