INTRODUCTION
[0001] Current approaches to treatment of cancer and other disorders may include combinations
of local therapies, such as surgery and radiation therapy, with systemic therapies,
such as chemotherapy and administration of pharmaceuticals or other agents. The therapeutic
success of any treatment is proportional to the delivered dose of the pharmaceutical
or other agent to the site in the subject where the cancer or disorder is localized.
The dose is limited by toxicity to normal tissue and delivery to the affected site
in the subject.
[0002] Controlled drug release is one method of allowing the use and specific delivery of
increased doses of the pharmaceutical or other agent to the affected site in the subject
while limiting toxicity caused by the pharmaceutical or agent. Despite numerous advances
in the area of controlled drug release to increase delivery of pharmaceuticals and
other agents to affected sites in a subject and to release the agents at the affected
site in a controlled manner, sufficient localized release of the agents to a localized
affected site remains problematic.
[0003] EP2255790A1 discloses a liposome composition comprising naproxen, and a method of obtaining same.
US2011/0105995A1 discloses uniform-sized, multi-drug carrying, and photosensitive liposomes for advanced
drug delivery.
SUMMARY
[0004] Compositions for controlled drug release and methods of using these compositions
are provided herein. The compositions are based on pH sensitive liposomes which are
capable of releasing a therapeutic agent at a target location and in a controllable
manner. The invention provides a pH sensitive lipid vesicle composition comprising
a lipid layer, the lipid layer comprising pH sensitive lipids, an agent, and an organic
halogen selected from chloral hydrate or bromal hydrate and having a concentration
of at least 0.1M in the vesicle, wherein exposure of the composition to ionizing radiation
allows the agent to be released from the lipid vesicle. The lipid vesicle is preferably
a liposome, and the agent may be a cancer therapeutic agent, a polypeptide, a toxin,
a radiotherapeutic agent, a radiosensitizing agent, an angiogenesis inhibitor, an
imaging agent or combinations thereof. The agent may be released in a controllable
fashion and in a localized manner depending on the amount of organic halogen incorporated
into the pH sensitive lipid vesicle and the intensity, duration and localized use
of ionizing radiation.
[0005] In another aspect of the invention, pharmaceutical compositions comprising the pH
sensitive lipid vesicles of the invention and a pharmaceutically acceptable carrier
are provided.
[0006] The compositions for use of the invention are defined by the claims.
[0007] Methods of delivering an agent to a target in a subject are also disclosed. These
methods include administering the pH sensitive lipid vesicle described herein to the
subject and then exposing the target to ionizing radiation, whereby the agent is released
from the lipid vesicle near the target in the subject.
[0008] Methods of controlled drug release are provided in which the pH sensitive lipid vesicles
described herein are administered to the subject and a localized area of the subject
is exposed to ionizing radiation causing the agent to be released in a localized area.
[0009] In a further aspect, the composition of the invention is provided for use as a medicament,
i.e. for use in methods of treating a condition. The methods may include administering
the pH sensitive lipid vesicles described herein to a subject with the condition and
exposing a portion of the subject to ionizing radiation. The agent is capable of treating
the condition and is released from the lipid vesicle in the portion of the subject
exposed to ionizing radiation. Suitably the portion of the subject exposed to ionizing
radiation is the portion of the subject in which the condition is in need of treatment
with the agent. In one aspect, such a treatment moderates or reduces at least one
toxic or negative side effect associated with treatment with the agent.
BRIEF DESCRIPTION OF THE DRAWINGS
[0010]
Figure 1 is a schematic diagram of a method for controlled drug release based on a
pH sensitive lipid vesicle. A lipid vesicle comprising pH sensitive components, such
as lipids or polymers, an aqueous environment, an agent and an organic halogen is
depicted. When exposed to ionizing radiation, such as X-rays, the organic halogen
reacts with water in the lipid vesicle to release H+, decrease the pH inside the vesicle
and cause the pH sensitive lipid vesicle to become permeable and release the agent.
Figure 2 is a set of graphs showing the release of a fluorescent marker, calcein,
from liposomes after exposure to X-rays over time. Figure 2A shows the results using
liposomes containing 1 M chloral hydrate. Figure 2B shows the results using liposomes
containing 0.5 M chloral hydrate. Figure 2C shows the results using liposomes containing
0.1 M chloral hydrate. In all three graphs, squares represent release from liposomes
after no irradiation exposure. The triangles and circles represent the release of
the fluorescent marker from the liposomes after exposure to X-rays. The triangles
represent liposomes that also contained 0.1M glucose in the vesicles.
DETAILED DESCRIPTION
[0011] The ability to release a therapeutic drug only at a desired location and in a controllable
manner is a crucial step in limiting the potential harmful systemic sequels inherent
to non-localized drug delivery. This challenge is yet unmet especially for cancer
therapy where the chemical dose is drastically limited by the remote toxicity. Ideally,
a drug delivery method would comprise drug transportation to the desired site in a
specialized vehicle which protects the drug from acting until an external controlled
stimulus triggers the drug release at the target site. Stealth liposomes are a good
example of a vehicle that can carry drug cargo in a protected state and evade detection
by the body's self defense mechanisms. However, the contained drug is not quickly
released only at the desired site and in a controllable manner. Local and controlled
drug release is a major challenge for drug therapy and various approaches have been
proposed to solve this issue.
[0012] One approach for drug delivery makes use of photo sensitive liposomes, which release
the trapped drug upon UV photolysis (Bisby et al. 1999; Bisby et al. 2000; Bisby et
al. 2000). Photoisomerization drives the photochromic lipid to a bulky state which
destabilizes the bilayer and increases its permeability for the entrapped drug. Although
very promising, this approach has limits imposed by the use of UV light as activator.
The human body is not transparent to UV radiation. The second drawback is given by
the high energy requirements for the UV laser pulse (cca 15 mJ/ 10 nsec). Another
approach is described in International Application No.
PCT/US2010/029413.
[0013] pH sensitive liposomes are one of the most studied and promising approaches for drug
delivery (Hazemoto et al. 1990; Kitano et al. 1991; Slepushkin et al. 1997; Lee et
al. 1998; Hafez et al. 2000). pH sensitive liposomes are able to deliver their payload
as a result of the variation of the internal or external pH, which destabilizes the
membrane and increases the permeability for the entrapped drug. A second drug delivery
mechanism is represented by their ability to fuse when exposed to the appropriate
pH. Although it has been supposed that the intra-tumor pH has lower values and this
is one of the main mechanisms of targeted drug release from these liposomes, this
assumption has never been satisfactorily proved. Use of pH sensitive liposome to deliver
agents to tumor tissues has not been widely demonstrated. On the other hand, the internal
pH suffers from variability, and the release rate can be extremely low in many conditions.
Moreover, the pH sensitive liposomes can be potentially used only if the diseased
site (or the desired location) experiences strong enough pH changes.
[0014] The pH sensitive lipid vesicles described herein utilize ionizing radiation to trigger
release of the agent only at the irradiated site. The described controlled drug delivery
scheme makes use of pH sensitive liposomes loaded with an agent and an organic halogen.
The liposomes or lipid vesicles are designed to release the agent upon interaction
with highly localized therapeutic X-ray radiation. In particular, the actinic beam
will be used to control the pH inside the liposomes. A decrease in pH inside the vesicle
will be triggered due to the aqueous solution of organic halogens inside the vesicle
yielding an increased acidity upon interaction with ionizing radiation (Andrews and
Shore 1950; Freeman et al. 1953; Freeman et al. 1953; Andrews et al. 1957; Woods and
Spinks 1957; Heusinger et al. 1959; Platford and Spinks 1959; Woods and Spinks 1960).
[0015] Briefly, the agent and a radiation sensitive compound (such as an organic halogen)
are encased in the water interior of a pH sensitive lipid vesicle such as a liposome.
The free radicals released by radiolysis interact with the organic halogen and increase
the acidity, i.e. free protons, inside the vesicles. The protons interact with the
pH sensitive components in the lipid membrane (lipids and/or polymers) and the agent
is released as a consequence of the increased permeability of the membrane.
[0016] Thus the lipid vesicles described herein may be administered to a subject and the
agent may be sequestered within the lipid vesicle until the subject or a target area
on or in the subject is exposed to ionizing radiation, thereby effecting release of
the agent from the lipid vesicle in a subject or in a target area of the subject.
The compositions may be used for controlled release of the agent to a target, such
as a target tissue, in a localized area. For example, the compositions may be used
to deliver an agent to a tumor, damaged tissue, site of infection or site of inflammation.
[0017] The lipid vesicles encasing all the components required for drug delivery can have
a variety of compositions and internal aqueous contents. The lipid vesicles can be
micelles, microspheres or liposomes. Liposomes may be unilamellar or multilamellar.
The lipid vesicles are suitably unilamellar, sterically stabilized, stealth, size
adjusted for a reduced absorption by the mononuclear phagocyte system (MPS), and the
lipidic phase should contain lipids or polymers assuring pH sensitivity. The lipid
vesicles may be made of any suitable lipid, including but not limited to, polar lipids,
such as phospholipids, such as phosphoglycerides, such as phosphatidylethanolamine,
phophatidylcholine, phosphatidylserine, cardiolipin, palmitoylhomocysteine, cholesterol
or combinations thereof. Other lipid moieties may also be included in the lipid vesicles
such as triacylglycerols, waxes, sphingolipids, and sterols and their fatty acid esters,
or combinations thereof. The lipid vesicle's lipid layer may also include polypeptides,
such as transmembrane polypeptides, protein channels or other polypeptides capable
of associating with or localizing to the lipid layer.
[0018] The lipid vesicles are pH sensitive lipid vesicles. Those of skill in the art will
appreciate many pH sensitive lipids and pH sensitive polymers are available to generate
pH sensitive lipid vesicles. The pH sensitive lipids may include, but are not limited
to phosphatidylethanolamine, palmitoylhomocysteine or analogs or derivatives thereof,
such as dioleoylphosphatidylethanolamine. pH sensitive polymers may include a pH sensitive
moiety including, but not limited to alklyacrylic acid, ethylacrylic acid, propylacrylic
acid, butylacrylic acid, and methylacrylic acid. See
U.S. Patent Nos. 7,951,846,
7,655,468,
7,186,399,
6,426,086 and
5,283,122 and
U.S. Patent Publication Nos. 20110027171,
20100129430, and
20100009926. The pH sensitive lipids or polymers may be included in the lipid vesicle at between
1 and 25 mol%, suitably 2-20 mol %, suitably 2-5 mol %. The actual composition and
lipid ratio in the pH sensitive lipid vesicles may be varied and should be chosen
in such a way as to initiate a sustained release at the achieved pH while sealing
at the initial pH.
[0019] The lipid vesicles may additionally include a functional group such as a targeting
molecule or polyethylene glycol. The targeting molecule or polyethylene glycol may
be exposed on or attached to the outer surface of the liposome to target the lipid
vesicle to a specific tissue or to increase the half- life of the lipid vesicle after
administration to a subject. The targeting molecule may be a polypeptide or protein.
Suitable targeting molecules include, but are not limited to, an antibody, a receptor
such as the folate receptor or a ligand for a receptor that helps target the lipid
vesicle to a specific tissue. The targeting molecule may be attached to the outer
surface of the lipid vesicle, be a peripheral or integral membrane protein or may
be attached by other means known to those skilled in the art such as myristoylation,
acetylation, prenylation, palmitoylation, glycosylphosphatidyinositol (GPI) anchors
or via cholesterol.
[0020] The liposome may be made by any method, including those known to those skilled in
the art, such as sonication, extrusion, reverse phase evaporation, the infusion procedure,
detergent dilution and the Mozifari method. Suitably the lipid vesicles are made by
extrusion. In the Examples, the liposomes were made by extrusion. Suitably, the liposomes
are at least about 20nm, 100nm, 200nm, 250nm in diameter. Suitably the liposomes are
no larger than about 400nm, 500nm, 1 micron, 2 microns or 4 microns in diameter. The
size of the liposome may depend on its selected use. Suitably, the liposomes are of
a size such that they are capable of selectively extravasating from leaky blood vessels
near a tumor site or a site of inflammation.
[0021] The radiation sensitive compound, suitably an organic halogen, should be added in
the desired concentration during preparation of the lipid vesicles. The agent can
be added during lipid vesicle preparation (passive loading) or after preparation (active
or passive loading). The unincorporated components can be removed by centrifugation,
dialysis, gel column filtration, etc. The lipid vesicles suitably are prepared with
and contain an aqueous media. The aqueous medium can be water or water-based and may
also contain salts, sugars, such as glucose, or other additives that may serve to
stabilize the vesicle, make the vesicle more pH sensitive or affect the activity of
the agent.
[0022] The agent controlled release process is initiated by ionizing radiation interacting
with water in the aqueous media of the lipid vesicle. Without being bound by theory,
the primary interaction takes place between X-ray photons and water, and the Compton
Effect dominates in this energy range for low Z. Organic halogen compounds, such as
chloral hydrate, are some of the most sensitive to ionizing radiation. The hydrogen
halide formed in high yield acidifies the aqueous solution of the lipid vesicle by
decreasing the pH. The acid formation is dependent on the organic halogen concentration,
radiation dose amount, radiation dose time of exposure, and temperature. Those of
skill in the art will understand that the concentration and ionizing radiation dose
will depend on the tissue being treated, agent being delivered and subject as well
as other factors. The radiolysis of organic halogenated compounds in aqueous solution
is a chain reaction; it has been extensively proved that the interaction with radiation
results in the formation of hydrogen halide and the acid produced modulates the solutions'
pH. The reaction mechanisms and the reaction products (other than acids) are not known
in detail but the action is undoubtedly indirect, through the radicals produced in
water by the actinic beam. The energy loss in the therapeutic energy range for water
solutions is due entirely to the Compton effect; the high yield of the acid formation
(at least 100 molecules/ion pair) can be explained only if we account for a chain
reaction in the acid formation mechanism. This high yield is essential in modulating
the internal pH of liposomes using rather modest radiation doses. The acid produced
can decrease the solution pH by a few units, more than enough to destabilize the lipid
bilayer and release the agent, or to deliver the agent by promoting the fusion of
the pH sensitive liposomes with cells. In the Examples, 6MeV X-ray, 400Rad, 2 minutes
duration was sufficient to mediate release of the agent from liposome comprising 0.1M
to 1M chloral hydrate. Those of skill in the art will understand that the amount of
agent released within a particular time after exposure to radiation will be related
to the particular dose of ionizing radiation provided and the amount of organic halopgen
incorporated in the liposome along with other factors. Doses of radiation may be any
dosage used to treat individuals without causing undue toxicity. Suitably the dose
of radiation may be between 200 and 600 Rad.
[0023] The radiation sensitive agent used herein is an organic halogen selected from chloral
hydrate, or bromal hydrate (Woods and Spinks 1957; Heusinger, Woods et al. 1959; Woods
and Spinks 1960). The amount of organic halogen added to the lipid vesicles is dependent
on the pH necessary to allow permeabilization of the lipid vesicle and the dose of
ionizing radiation that will be used, and the organic halogen has a concentration
of at least 0.1 M in the vesicle. Generally a lower amount of organic halogen will
lead to a lower change in pH in the lipid vesicle after exposure to ionizing radiation.
In the Examples, 0.1 M to 1M chloral hydrate was added to the liposomes. Suitably
the amount of organic halogen added is between 0.01M and 2M, suitably from 0.1 to
1M. As demonstrated in the Examples glucose can be added to the lipid vesicle to increase
the efficiency of release from the vesicles. Glucose can be added between 0.01 M and
1 M. Suitably glucose is added at between 0.1 M and 1 M.
[0024] The agent contained within the lipid vesicles may be any suitable pharmaceutical
or other active agent, including but not limited to, chemotherapeutic agents, toxins,
radiotherapeutic agents, radiosensitizing agents, imaging agents, tracking agents,
antimicrobials, anti-inflammatory agents, polypeptides such as recombinant proteins,
cytokines or antibodies, small chemicals or any combination thereof. In alternative
embodiments, the active agent may be an angiogenesis inhibitor or an apoptosis inducer.
[0025] Illustrative anticancer drugs that may be administered include acivicin, aclarubicin,
acodazole, acronycine, adozelesin, alanosine, aldesleukin, allopurinol sodium, altretamine,
aminoglutethimide, amonafide, ampligen, amsacrine, androgens, anguidine, aphidicolin
glycinate, asaley, asparaginase, 5-azacitidine, azathioprine, Bacillus calmette-guerin
(BCG), Baker's Antifol (soluble), beta-2'-deoxythioguanosine, bisantrene HCL, bleomycin
sulfate, busulfan, buthionine sulfoximine, BWA 773U82, BW 502U83.HCl, BW 7U85 mesylate,
ceracemide, carbetimer, carboplatin, carmustine, chlorambucil, chloroquinoxaline-sulfonamide,
chlorozotocin, chromomycin A3, cisplatin, cladribine, corticosteroids, Corynebacterium
parvum, CPT-11, crisnatol, cyclocytidine, cyclophosphamide, cytarabine, cytembena,
dabis maleate, dacarbazine, dactinomycin, daunorubicin HCl, deazauridine, dexrazoxane,
dianhydrogalactitol, diaziquone, dibromodulcitol, didemnin B, diethyldithiocarbamate,
diglycoaldehyde, dihydro-5-azacytidine, doxorubicin, echinomycin, edatrexate, edelfosine,
eflornithine, Elliott's solution, elsamitrucin, epirubicin, esorubicin, estramustine
phosphate, estrogens, etanidazole, ethiofos, etoposide, fadrazole, fazarabine, fenretinide,
filgrastim, finasteride. Flavone acetic acid, floxuridine, fludarabine phosphate,
5-fluorouracil, Fluosol.RTM., flutamide, gallium nitrate, gemcitabine, goserelin acetate,
hepsulfam, hexamethylene bisacetamide, homoharringtonine, hydrazine sulfate, 4-hydroxyandrostenedione,
hydrozyurea, idarubicin HCl, ifosfamide, interferon alfa, interferon beta, interferon
gamma, interleukin-1 alpha and beta, interleukin-3, interleukin-4, interleukin-6,
4-ipomeanol, iproplatin, isotretinoin, leucovorin calcium, leuprolide acetate, levamisole,
liposomal daunorubicin, liposome encapsulated doxorubicin, lomustine, lonidamine,
maytansine, mechlorethamine hydrochloride, melphalan, menogaril, merbarone, 6-mercaptopurine,
mesna, methanol extraction residue of Bacillus calmette-guerin, methotrexate, N-methylformamide,
mifepristone, mitoguazone, mitomycin-C, mitotane, mitoxantrone hydrochloride, monocyte/macrophage
colony-stimulating factor, nabilone, nafoxidine, neocarzinostatin, octreotide acetate,
ormaplatin, oxaliplatin, paclitaxel, pala, pentostatin, piperazinedione, pipobroman,
pirarubicin, piritrexim, piroxantrone hydrochloride, PIXY-321, plicamycin, porfimer
sodium, prednimustine, procarbazine, progestins, pyrazofurin, razoxane, sargramostim,
semustine, spirogermanium, spiromustine, streptonigrin, streptozocin, sulofenur, suramin
sodium, tamoxifen, taxotere, tegafur, teniposide, terephthalamidine, teroxirone, thioguanine,
thiotepa, thymidine injection, tiazofurin, topotecan, toremifene, tretinoin, trifluoperazine
hydrochloride, trifluridine, trimetrexate, tumor necrosis factor, uracil mustard,
vinblastine sulfate, vincristine sulfate, vindesine, vinorelbine, vinzolidine, Yoshi
864, zorubicin, and mixtures thereof.
[0026] Exposure of the compositions described herein to ionizing radiation allows the agent
to be released from the lipid vesicles by decreasing the pH and causing the lipid
vesicle to become permeable as described above. Suitably, exposure of the lipid vesicles
to ionizing radiation releases at least 25% of the agent within 4 hours after exposure.
More suitably, exposure of the lipid vesicle to ionizing radiation releases at least
50% of the agent within 8 hours after exposure. More suitably, exposure of the lipid
vesicle to ionizing radiation releases at least 75% of the agent within 8 hours after
exposure. More suitably, exposure of the lipid vesicle to ionizing radiation releases
at least 90% of the agent within 8 hours after exposure.
[0027] The lipid vesicle compositions described herein may be used to make pharmaceutical
compositions. Pharmaceutical compositions comprising the compositions described above
and a pharmaceutically acceptable carrier are also provided. A pharmaceutically acceptable
carrier is any carrier suitable for
in vivo administration. Examples of pharmaceutically acceptable carriers suitable for use
in the composition include, but are not limited to, water, buffered solutions, saline,
glucose or dextrose solutions, glycerol, oil-based or bacterial culture fluids. Additional
components of the compositions may suitably include, for example, excipients such
as stabilizers, preservatives, diluents, emulsifiers and lubricants. Examples of pharmaceutically
acceptable carriers or diluents include stabilizers such as carbohydrates (e.g., sorbitol,
mannitol, starch, sucrose, glucose, dextran), proteins such as albumin or casein,
protein-containing agents such as bovine serum or skimmed milk and buffers (e.g.,
phosphate buffer). Especially when such stabilizers are added to the compositions,
the composition is suitable for freeze-drying or spray-drying. The composition may
also be prepared in conventional forms, either as a liquid solution or suspension
or in a solid form suitable for preparation as a solution or suspension in a liquid
prior to injection, or as an emulsion.
[0028] Methods of delivering an agent to a target in a subject are also disclosed. The methods
include administering the lipid vesicles described herein to a subject and exposing
the target to ionizing radiation. The ionizing radiation and the organic halogen interact
to reduce the pH of the lipid vesicle and destabilize or increase the permeability
of the lipid vesicle resulting in the release of the agent from the lipid vesicle
at the target site to result in controlled delivery of the agent to the target. The
target may be a specific tissue or organ such as the liver, kidneys, spleen or may
be the site of a tumor, infection, inflammation or other site of disease. Suitably,
the lipid vesicle is administered prior to administration of the ionizing radiation.
Suitably, the ionizing radiation source is X-rays as used in the Examples. Other suitable
sources of ionizing radiation include gamma-rays and beta-rays. Proton therapy is
a promising approach for cancer therapy and proton therapy will also provide a useful
source of ionizing radiation. The methods include methods of controlled drug release
in which the agent is released from the lipid vesicles in a localized area within
the subject and/or at a specific time.
[0029] Methods of treating a condition, suitably a medical condition, by administering the
lipid vesicle compositions described herein to a subject in need of treatment for
the condition. The subject or a portion of the subject is then exposed to ionizing
radiation. The agent is capable of treating the condition when released from the pH
sensitive lipid vesicle in the portion of the subject exposed to ionizing radiation.
By releasing the agent only in or near the portion of the subject exposed to ionizing
radiation, the dose of the agent may be increased locally without adverse side-effects
that may be associated with treatment with the agent systemically. Thus agents whose
side-effects limit the effective dosage range may be used at higher effective doses
in combination with the pH sensitive lipid vesicles described herein. Alternatively,
many agents have adverse side-effects that are not associated with the effectiveness
of the agent. The methods described herein may result in decreased side-effects associated
with treatment with the agent.
[0030] The lipid vesicle or liposome is generally administered to the subject prior to administration
of the ionizing radiation, which triggers release of the active agent, but the compositions
and ionizing radiation may be administered simultaneously in certain circumstances.
Suitably the lipid vesicle or liposome is administered at 10 minutes, 30 minutes,
1 hour, 2 hours, 4 hours, 8 hours, 24 hours, 48 hours, 3 days, 4 days, 5 days, 6 days
or up to 7 days prior to administration of ionizing radiation. The compositions may
be administered locally or systemically. The lipid vesicle may be administered by
any means available, including those known to those skilled in the art, such as intravenous,
intratumoral, intraperitoneal, intramuscular, intra-arterial, intraventricular, subcutaneous,
dermal, or transdermal delivery. The lipid vesicle may be exposed to ionizing radiation
by exposing the subject or a part of the subject to the radiation. Suitably, the agent
is administered in an amount effective to treat the condition and achieve a therapeutic
effect in the subject, e.g., an antineoplastic effect.
[0031] The methods may be used to treat subjects having one or more of a wide variety of
medical conditions, including but not limited to cancer, such as meningiomas, hepatic
cell carcinoma, pancreatic tumors, infectious diseases including fungal, bacterial,
or parasitic diseases, inflammatory diseases including psoriasis and arthritis and
atrial-ventricular malformations. Treatment of cancer includes, but is not limited
to, killing cancer cells, slowing metastases, slowing or stopping angiogenesis, slowing
or stopping the growth of the cancer cells or the size or mass of a tumor, making
the cancer more responsive to a secondary therapy, such as radiation treatment. Treating
an infectious disease includes, but is not limited to, reducing the length or severity
of the infection, reducing morbidity, reducing mortality or killing the infectious
agent. Treating an inflammatory disease includes, but is not limited to, reducing
the amount or size of inflammation, reducing the severity of the inflammation, and
reducing the length of the inflammatory outbreak.
[0032] The pH sensitive vesicles or liposomes may also comprise an agent selected from an
imaging agent such as a fluorescent marker, a dye, a radiological marker or other
tracking agent. These vesicles may be used in methods of tracking or determining the
dosage or exposure to ionizing radiation. For example the tracking agent may be used
as a visual aid to ensure that the proper dosage of ionizing radiation was administered
to a subject to reach a desired site or cause a desired reaction.
[0033] Administration of the compositions described herein to a subject appears to exhibit
beneficial effects in a dose-dependent manner. Thus, within broad limits, administration
of larger quantities of the compositions is expected to achieve increased beneficial
biological effects than administration of a smaller amount. Moreover, efficacy is
also contemplated at dosages below the level at which toxicity associated with the
agent is seen.
[0034] It will be appreciated that the specific dosage administered in any given case will
be adjusted in accordance with the compositions being administered, the disease to
be treated or inhibited, the condition of the subject, and other relevant medical
factors that may modify the activity of the compositions or the response of the subject,
as is well known by those skilled in the art. For example, the specific dose for a
particular subject depends on age, body weight, general state of health, diet, the
timing and mode of administration, the rate of excretion, medicaments used in combination
and the severity of the particular disorder to which the therapy is applied. Dosages
for a given patient can be determined using conventional considerations, e.g., by
customary comparison of the differential activities of the compositions described
herein and of the agent provided without a lipid vesicle delivery vehicle, such as
by means of an appropriate conventional pharmacological or prophylactic protocol.
[0035] The maximal dosage for a subject is the highest dosage that does not cause undesirable
or intolerable side effects. The number of variables in regard to an individual prophylactic
or treatment regimen is large, and a considerable range of doses is expected. The
route of administration will also impact the dosage requirements. It is anticipated
that dosages of the compositions will reduce symptoms of the condition at least 10%,
20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% or 100% compared to pre-treatment symptoms
or symptoms is left untreated. It is specifically contemplated that pharmaceutical
preparations and compositions may palliate or alleviate symptoms of the disease without
providing a cure, or, in some embodiments, may be used to cure the disease or disorder.
[0036] Suitable effective dosage amounts for administering the compositions may be determined
by those of skill in the art, but typically range from about 1 microgram to about
100,000 micrograms per kilogram of body weight of the agent weekly, although they
are typically about 1,000 micrograms or less per kilogram of body weight weekly. In
some embodiments, the effective dosage amount ranges from about 10 to about 10,000
micrograms per kilogram of body weight weekly. In another embodiment, the effective
dosage amount ranges from about 50 to about 5,000 micrograms per kilogram of body
weight weekly. In another embodiment, the effective dosage amount ranges from about
75 to about 1,000 micrograms per kilogram of body weight weekly. The effective dosage
amounts described herein refer to total amounts administered, that is, if more than
one agent is administered, the effective dosage amounts correspond to the total amount
administered. The compositions can be administered as a single dose or as divided
doses. For example, the composition may be administered two or more times separated
by 4 hours, 6 hours, 8 hours, 12 hours, a day, two days, three days, four days, one
week, two weeks, or by three or more weeks. The number of liposomes or vesicles in
a dose will depend largely on the amount of the agent per liposome or per mL of the
solution.
[0037] The following examples are meant only to be illustrative and are not meant as limitations
on the scope of the invention or of the appended claims.
EXAMPLES
[0038] Pharmaceuticals, therapeutics or other agents can be released from pH sensitive liposomes
when the external or internal pH decreases depending on the liposomes. The liposomes
are pH sensitive and include an organic halogen in the liposome. The pH of an organic
halogen solution decreases upon X-ray irradiation. The decreased pH facilitates drug
release by increasing the liposomal membrane permeability of the pH sensitive vesicles.
Materials and Methods
[0039] pH sensitive liposomes which are stable at neutral pH but become destabilized at
slightly acid pH were prepared as previously described by extrusion through polycarbonate
filters (Hafez, 2000 and in International PCT Application No.
PCT/US2010/029413). The liposomes were composed of phosphatidylethanolamine, distearoylphosphatidylcholine,
cholesterol, and 1,2-distearoyl-
sn-glycero-3-phosphoethanolamine-N-(methoxy(polyethylene glycol)-2000] in a molar ratio
of 1:0.5:0.5:0.075 in unbuffered chloral hydrate (C
2H
3Cl
3O
2) solutions of 1M, 0.5M, and 0.1 M, with or without addition of 0.1M glucose (pH was
adjusted to 7.1 for all samples). The fluorescent drug simulator, calcein, was added
during liposome preparation at 5mM concentration, sufficient for fluorescence self
quenching. Control samples with identical composition but without glucose were prepared
at the same time with the test samples. All test samples were irradiated with 6MeV
X-ray, 400Rad, 2 minutes (therapeutic dose). The fluorescence as indicator of drug
release was measured before and after irradiation with a Horiba fluorometer (Fluoromax
4P) in kinetics mode. The maximum release was estimated from the fluorescence measured
upon complete membrane destabilization with 0.2% Triton X-100.
Results
[0040] The results are presented in Figure 2. The dashed line represents release of calcein
after treatment of the vesicles with 0.2% Triton X-100 and is considered 100% release.
All test samples indicated extensive calcein release upon irradiation. As a general
rule, addition of glucose at 0.1 M increased slightly the efficiency of release for
all samples, and this confirms previous experimentation showing that glucose addition
facilitates pH decreases upon irradiation (Hilsenrod 1956). The non-irradiated sample
containing 1M chloral hydrate showed some calcein leakage at rest (Figure 2a) and
suggested that at this concentration the liposomes are not highly stable. The samples
containing 0.5M (Figure 2b) and 0.1M (Figure 2c) chloral hydrate showed a higher stability
and minimal or no leakage at rest. Both the samples including 0.5M or 0.1M chloral
hydrate showed over 50% release of the calcein by 8 hours after the exposure to X-rays.
Conclusions
[0041] We demonstrated that X-ray radiation can control the release of drugs from pH sensitive
liposomes containing organic halogen solutions. Further optimization will focus on
using various lipid compositions for faster release and improved stability at rest
and stability and controlled release in the context of a subject.
References
[0042]
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1. A pH sensitive lipid vesicle composition comprising a lipid layer, the lipid layer
comprising pH sensitive lipids, an agent, and an organic halogen selected from chloral
hydrate or bromal hydrate and having a concentration of at least 0.1M in the vesicle,
wherein exposure of the composition to ionizing radiation allows the agent to be released
from the lipid vesicle, wherein the lipid vesicle is preferably a liposome, and optionally
wherein the agent is a cancer therapeutic agent, a polypeptide, a toxin, a radiotherapeutic
agent, a radiosensitizing agent, an angiogenesis inhibitor, an imaging agent or combinations
thereof.
2. The composition of claim 1, wherein the lipid layer comprises lipids selected from
phosphatidylethanolamine, palmitoylhomocysteine, diacylsuccinylglycerols, dioleoylphosphatidylethanolamine,
or analogs or derivatives thereof.
3. The composition of any one of claims 1-2, wherein the lipid layer comprises pH sensitive
polymers with a moiety selected from alklyacrylic acid, ethylacrylic acid, propylacrylic
acid, butylacrylic acid, and methylacrylic acid.
4. The composition of any one of claims 1-3, wherein the lipid layer comprises lipid
anchored polyethylene glycol, cholesterol, or at least one polypeptide capable of
localizing in the lipid layer.
5. The composition of any one of claims 1-4, further comprising a targeting molecule,
and optionally wherein the targeting molecule is selected from a targeting polypeptide,
a folate receptor, an antibody or combinations thereof.
6. The composition of any one of claims 1-5, wherein the organic halogen is chloral hydrate.
7. A pharmaceutical composition comprising the pH sensitive lipid vesicle composition
of any one of claims 1-6 and a pharmaceutically acceptable carrier.
8. The composition of any one of claims 1-6 for use in a method for treatment of a subject
by therapy, wherein the composition delivers an agent to a target in the subject,
the method comprising:
a. Administering the composition to the subject and
b. Exposing the target to ionizing radiation, whereby the agent is released from the
lipid vesicle near the target in the subject, and optionally wherein the target comprises
a tumor,
wherein the subject has one or more medical conditions selected from cancer, inflammatory
disease, fungal, bacterial, and/or parasitic disease.
9. The composition for use according to claim 8, wherein the ionizing radiation source
is X-rays or gamma rays.
10. The composition for use according to claim 8, wherein the lipid vesicle is administered
prior to exposing the target to ionizing radiation.
11. The composition for use according to any one of claims 8-10 in a method of controlled
drug release, the method comprising the method of any one of claims 8-10, wherein
the agent is released in a localized area.
12. The composition of any one of claims 1-6 for use as a medicament.
13. The composition of any one of claims 1-7 for use in a method of treating a condition
comprising administering the composition of any one of claims 1-7 to a subject with
the condition and exposing a portion of the subject to ionizing radiation, wherein
the agent is capable of treating the condition and whereby the agent is released from
the lipid vesicle in the portion of the subject exposed to ionizing radiation, and
wherein the condition is cancer.
14. The composition for use according to claim 13, wherein the use reduces the side-effects
associated with treatment of the agent in the absence of the lipid vesicle.
1. pH-empfindliche Lipidvesikel-Zusammensetzung, die eine Lipidschicht umfasst, wobei
die Lipidschicht pH-empfindliche Lipide, ein Mittel und ein organisches Halogen umfasst,
das aus Chloralhydrat oder Bromalhydrat ausgewählt ist, und eine Konzentration von
zumindest 0,1 M in dem Vesikel aufweist, wobei das Aussetzen der Zusammensetzung gegenüber
einer Ionenstrahlung die Freisetzung des Mittels aus dem Lipidvesikel ermöglicht,
wobei das Lipidvesikel vorzugsweise ein Liposom ist und wobei das Mittel vorzugsweise
ein Krebstherapeutikum, ein Polypeptid, ein Toxin, ein Radiotherapeutikum, ein Radiosensibilisierungsmittel,
ein Angiogenesehemmer, ein Bildgebungsmittel oder eine Kombination davon ist.
2. Zusammensetzung nach Anspruch 1, wobei die Lipidschicht Lipide umfasst, die aus Phosphatidylethanolamin,
Palmitoylhomocystein, Diacylsuccinylglycerinen, Dioleoylphosphatidylethanolamin und
Analoga oder Derivaten davon ausgewählt sind.
3. Zusammensetzung nach einem der Ansprüche 1 bis 2, wobei die Lipidschicht pH-empfindliche
Polymere mit einer Gruppierung umfasst, die aus Alkylacrylsäure, Ethylacrylsäure,
Propylacrylsäure, Butylacrylsäure und Methylacrylsäure ausgewählt ist.
4. Zusammensetzung nach einem der Ansprüche 1 bis 3, wobei die Lipidschicht lipidverankertes
Polyethylenglykol, Cholesterin oder zumindest ein Polypeptid umfasst, das zur Lokalisierung
in der Lipidschicht in der Lage ist.
5. Zusammensetzung nach einem der Ansprüche 1 bis 4, die außerdem ein abzielendes Molekül
umfasst, wobei das abzielende Molekül gegebenenfalls aus einem abzielenden Polypeptid,
einem Folatrezeptor, einem Antikörper und Kombinationen davon ausgewählt ist.
6. Zusammensetzung nach einem der Ansprüche 1 bis 5, wobei das organische Halogen Choralhydrat
ist.
7. Pharmazeutische Zusammensetzung, die eine pH-empfindliche Lipidvesikel-Zusammensetzung
nach einem der Ansprüche 1 bis 6 und einen pharmazeutisch annehmbaren Träger umfasst.
8. Zusammensetzung nach einem der Ansprüche 1 bis 6 zur Verwendung in einem Verfahren
zur Behandlung eines Individuums mittels Therapie, wobei die Zusammensetzung ein Mittel
zu einem Ziel in dem Individuum zuführt, wobei das Verfahren Folgendes umfasst:
a) das Verabreichen der Zusammensetzung an das Individuum und
b) das Aussetzen des Ziels gegenüber einer Ionenstrahlung, wobei das Mittel aus dem
Lipidvesikel in der Nähe des Ziels im Individuum freigesetzt wird, wobei das Ziel
gegebenenfalls einen Tumor umfasst,
wobei das Individuum eine oder mehrere Krankheiten aufweist, die aus Krebs, einer
Entzündungserkrankung, einer Pilz-, Bakterien- und/oder Parasitenerkrankung ausgewählt
ist.
9. Zusammensetzung zur Verwendung nach Anspruch 8, wobei die Ionenstrahlenquelle Röntgenstrahlen
oder Gammastrahlen sind.
10. Zusammensetzung zur Verwendung nach Anspruch 8, wobei das Lipidvesikel vor dem Aussetzen
des Ziels gegenüber der Ionenstrahlung verabreicht wird.
11. Zusammensetzung zur Verwendung nach einem der Ansprüche 8 bis 10 in einem Verfahren
einer kontrollierten Arzneimittelfreisetzung, wobei das Verfahren ein Verfahren nach
einem der Ansprüche 8 bis 10 umfasst, wobei das Mittel in dem lokalisierten Bereich
freigesetzt wird.
12. Zusammensetzung nach einem der Ansprüche 1 bis 6 zur Verwendung als Medikament.
13. Zusammensetzung nach einem der Ansprüche 1 bis 7 zur Verwendung in einem Verfahren
zur Behandlung eines Leidens, welches das Verabreichen einer Zusammensetzung nach
einem der Ansprüche 1 bis 7 an ein Individuum mit dem Leiden und das Aussetzen eines
Teils des Individuums gegenüber Ionenstrahlung umfasst, wobei das Mittel zur Behandlung
des Leidens in der Lage ist und wobei das Mittel aus dem Lipidvesikel in dem der Ionenstrahlung
ausgesetzten Teil des Individuums freigesetzt wird und wobei das Leiden Krebs ist.
14. Zusammensetzung zur Verwendung nach Anspruch 13, wobei die Verwendung die mit einer
Behandlung des Mittels ohne das Lipidvesikel in Zusammenhang stehenden Nebenwirkungen
verringert.
1. Composition de vésicule lipidique sensible au pH comprenant une couche lipidique,
la couche lipidique comprenant des lipides sensibles au pH, un agent, et un halogène
organique choisi parmi de l'hydrate de chloral ou hydrate de bromal et ayant une concentration
d'au moins 0,1 M dans la vésicule, dans laquelle l'exposition de la composition à
un rayonnement ionisant permet à l'agent d'être libéré de la vésicule lipidique, la
vésicule lipidique étant de préférence un liposome, et facultativement dans laquelle
l'agent est un agent thérapeutique anticancéreux, un polypeptide, une toxine, un agent
radiothérapeutique, un agent radio-sensibilisant, un inhibiteur d'angiogenèse, un
agent d'imagerie ou des combinaisons de ceux-ci.
2. Composition selon la revendication 1, dans laquelle la couche lipidique comprend des
lipides choisis parmi de la phosphatidyléthanolamine, palmitoylhomocystéine, diacylsuccinylglycérols,
dioléoylphosphatidyléthanolamine, ou des analogues ou dérivés de ceux-ci.
3. Composition selon l'une quelconque des revendications 1 à 2, dans laquelle la couche
lipidique comprend des polymères sensibles au pH avec un fragment choisi parmi de
l'acide alkylacrylique, acide éthylacrylique, acide propylacrylique, acide butylacrylique,
et acide méthylacrylique.
4. Composition selon l'une quelconque des revendications 1 à 3, dans laquelle la couche
lipidique comprend du polyéthylène glycol ancré à des lipides, du cholestérol, ou
au moins un polypeptide capable d'une localisation dans la couche lipidique.
5. Composition selon l'une quelconque des revendications 1 à 4, comprenant en outre une
molécule de ciblage, et facultativement dans laquelle la molécule de ciblage est choisie
parmi un polypeptide de ciblage, un récepteur de folate, un anticorps ou des combinaisons
de ceux-ci.
6. Composition selon l'une quelconque des revendications 1 à 5, dans laquelle l'halogène
organique est de l'hydrate de chloral.
7. Composition pharmaceutique comprenant la composition de vésicule lipidique sensible
au pH selon l'une quelconque des revendications 1 à 6 et un support pharmaceutiquement
acceptable.
8. Composition selon l'une quelconque des revendications 1 à 6 pour une utilisation dans
un procédé de traitement d'un sujet par thérapie, dans laquelle la composition délivre
un agent à une cible dans le sujet, le procédé comprenant les étapes consistant à
:
a. administrer la composition au sujet et
b. exposer la cible à un rayonnement ionisant, de sorte que l'agent est libéré de
la vésicule lipidique proche de la cible chez le sujet, et facultativement dans laquelle
la cible comprend une tumeur,
dans laquelle le sujet a une ou plusieurs affections médicales choisies parmi un cancer,
une maladie inflammatoire, une maladie fongique, bactérienne et/ou parasitaire.
9. Composition pour une utilisation selon la revendication 8, dans laquelle la source
de rayonnement ionisant est constituée de rayons X ou de rayons gamma.
10. Composition pour une utilisation selon la revendication 8, dans laquelle la vésicule
lipidique est administrée avant d'exposer la cible à un rayonnement ionisant.
11. Composition pour une utilisation selon l'une quelconque des revendications 8 à 10
dans un procédé de libération de médicament régulée, le procédé comprenant le procédé
selon l'une quelconque des revendications 8 à 10, dans lequel l'agent est libéré dans
une zone localisée.
12. Composition selon l'une quelconque des revendications 1 à 6 pour une utilisation comme
médicament.
13. Composition selon l'une quelconque des revendications 1 à 7 pour une utilisation dans
un procédé de traitement d'un état comprenant l'administration de la composition selon
l'une quelconque des revendications 1 à 7 à un sujet présentant l'état et l'exposition
d'une partie du sujet à un rayonnement ionisant, dans laquelle l'agent est capable
de traiter l'état et de sorte que l'agent est libéré de la vésicule lipidique dans
la partie du sujet exposé à un rayonnement ionisant, et dans lequel l'état est un
cancer.
14. Composition pour une utilisation selon la revendication 13, dans laquelle l'utilisation
réduit les effets secondaires associés à un traitement de l'agent en l'absence de
la vésicule lipidique.