EP 0370103 A4 19911127 - PROTEASE-DEFICIENT GRAM-POSITIVE BACTERIA AND THEIR USE AS HOST ORGANISMS FOR THE PRODUCTION OF RECOMBINANT PRODUCTS
Title (en)
PROTEASE-DEFICIENT GRAM-POSITIVE BACTERIA AND THEIR USE AS HOST ORGANISMS FOR THE PRODUCTION OF RECOMBINANT PRODUCTS
Publication
Application
Priority
US 19048388 A 19880505
Abstract (en)
[origin: WO8910976A1] The residual protease activity in AP<->/NP<-> strains of Bacillus is due to two additional proteases, a residual serine protease (RSP) and a sulfhydryl-dependent residual cysteine protease (RCP), which together account for the degradation of recombinant proteins in cultures of B. subtilis (AP<->/NP<->). An analysis has been developed which allows for the identification and development of mutant strains of B. subtilis which are deficient in not only the neutral and alkaline proteases, but also in one or both of the residual proteases. These strains are highly suitable for use as host organisms for the production of heterologous proteins which are susceptible to the activity of the residual proteases.
IPC 1-7
IPC 8 full level
C12N 1/20 (2006.01); C12N 9/56 (2006.01); C12N 15/75 (2006.01); C12Q 1/37 (2006.01); C12Q 1/68 (2006.01); C12Q 1/689 (2018.01); C12R 1/07 (2006.01); C12R 1/125 (2006.01); C12R 1/445 (2006.01)
CPC (source: EP)
C12N 9/54 (2013.01); C12N 15/75 (2013.01); C12Q 1/37 (2013.01); C12Q 1/689 (2013.01); G01N 2333/32 (2013.01); G01N 2333/96466 (2013.01)
Citation (search report)
- [XD] WO 8706264 A1 19871022 - NEW YORK HEALTH RES INST [US]
- See references of WO 8910976A1
Designated contracting state (EPC)
AT BE CH DE FR GB IT LI LU NL SE
DOCDB simple family (publication)
WO 8910976 A1 19891116; AU 3765189 A 19891129; AU 622916 B2 19920430; DK 1590 A 19900205; DK 1590 D0 19900104; EP 0370103 A1 19900530; EP 0370103 A4 19911127; FI 900045 A0 19900104; FI 900045 A 19900104; HU 894054 D0 19900728; HU T53154 A 19900928; IL 89767 A0 19890928; JP H03500606 A 19910214; NZ 228424 A 19920625; PT 90463 A 19891130; ZA 892325 B 19900328
DOCDB simple family (application)
US 8901056 W 19890314; AU 3765189 A 19890314; DK 1590 A 19900104; EP 89906977 A 19890314; FI 900045 A 19900104; HU 405489 A 19890314; IL 8976789 A 19890328; JP 50624289 A 19890314; NZ 22842489 A 19890321; PT 9046389 A 19890504; ZA 892325 A 19890329