Global Patent Index - EP 0620860 A4

EP 0620860 A4 19970502 - PREPARATION OF RECOMBINANT BORRELIA PROTEINS.

Title (en)

PREPARATION OF RECOMBINANT BORRELIA PROTEINS.

Publication

EP 0620860 A4 19970502 (EN)

Application

EP 92922390 A 19921016

Priority

  • US 9208697 W 19921016
  • US 77904891 A 19911018
  • US 88876592 A 19920527

Abstract (en)

[origin: WO9308299A1] Highly-purified immunologically-effected recombinant protein encoded by a gene of a full-length wild-type Borrelia lipoprotein, particularly the OspA encoded by the OspA gene of B.burgdorferi, useful in vaccines and test kits for Lyme disease, is prepared by inducing protein production from a host organism transformed by a plasmid vector containing the cloned gene and subsequently recovering and purifying the protein. The grown organism is lysed and contacted with a surfactant, particularly Triton X-114, which selectively extracts the desired protein from the lysed cells. Upon heating the resulting mixture to mildly-elevated temperature, a detergent phase separates out and is recovered separate from the other phases. The detergent phase is further purified from residual protein by column chromatography.

IPC 1-7

C12P 21/06; C12Q 1/00; C07K 3/00; A61K 39/00

IPC 8 full level

A61K 39/00 (2006.01); A61P 31/04 (2006.01); C07K 1/14 (2006.01); C07K 1/16 (2006.01); C07K 14/00 (2006.01); C07K 14/20 (2006.01); C07K 16/00 (2006.01); C12N 15/09 (2006.01); C12P 21/02 (2006.01); C12P 21/06 (2006.01); C12Q 1/00 (2006.01); G01N 33/53 (2006.01); G01N 33/569 (2006.01); C12R 1/01 (2006.01); C12R 1/19 (2006.01)

CPC (source: EP)

A61P 31/04 (2018.01); C07K 14/20 (2013.01); A61K 39/00 (2013.01); Y02A 50/30 (2018.01)

Citation (search report)

  • [X] EP 0418827 A1 19910327 - MAX PLANCK GESELLSCHAFT [DE], et al
  • [X] WO 9109870 A1 19910711 - MIKROGEN MOLEKULARBIOL ENTW [DE]
  • [E] WO 9304175 A1 19930304 - SMITHKLINE BEECHAM BIOLOG [BE], et al
  • [X] SCHAIBLE UE ET AL.: "Monoclonal antibodies specific for the outer surface protein A (OspA) of Borrelia burgdorferi prevent Lyme borreliosis in severe combined immunodeficiency (scid) mice", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 87, May 1990 (1990-05-01), WASHINGTON US, pages 3768 - 3772, XP002025454
  • See also references of WO 9308299A1

Designated contracting state (EPC)

AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL SE

DOCDB simple family (publication)

WO 9308299 A1 19930429; AU 2801192 A 19930521; AU 676140 B2 19970306; CA 2121121 A1 19930429; CA 2121121 C 20030610; EP 0620860 A1 19941026; EP 0620860 A4 19970502; FI 941749 A0 19940415; FI 941749 A 19940525; IL 103462 A0 19930315; JP H06511154 A 19941215; NO 310417 B1 20010702; NO 941376 D0 19940415; NO 941376 L 19940617; PT 100980 A 19940131; PT 100980 B 19990730

DOCDB simple family (application)

US 9208697 W 19921016; AU 2801192 A 19921016; CA 2121121 A 19921016; EP 92922390 A 19921016; FI 941749 A 19940415; IL 10346292 A 19921018; JP 50775192 A 19921016; NO 941376 A 19940415; PT 10098092 A 19921016