Global Patent Index - EP 0658196 A4

EP 0658196 A4 19990707 - GENE INSERTION BY DIRECT LIGATION -i(IN VITRO).

Title (en)

GENE INSERTION BY DIRECT LIGATION -i(IN VITRO).

Title (de)

GENINSERTION DURCH DIREKTE -'(IN VITRO) LIGATION.

Title (fr)

INSERTION GENIQUE PAR LIAISON -i(IN VITRO) DIRECTE.

Publication

EP 0658196 A4 19990707 (EN)

Application

EP 94919295 A 19940527

Priority

  • US 9406079 W 19940527
  • US 7016493 A 19930528

Abstract (en)

[origin: WO9428114A1] A method is described for constructing recombinant double stranded DNA viruses, especially baculoviruses and granulosis viruses, by the direct ligation of DNA fragments in vitro. Also described are direct ligation virus vectors, which are insect viruses that have been modified by the insertion of at least one recognition site for a specific restriction endonuclease which does not cut the viral genome, so as to facilitate the insertion of foreign DNA segments by DNA ligation in vitro, and to the recombinant viruses formed by this direct ligation. Further described are modular expression vectors (plasmids) that are designed to facilitate the assembly of gene expression cassettes or other DNA fragments into virus insertion modules (as shown in the figure) which are inserted subsequently into direct ligation virus vectors at predefined sites and in a predefined orientation by ligation in vitro, and to recombinant viruses derived from said modular expression vectors.

IPC 1-7

C12N 7/00; C12N 7/01; C12N 15/00; C12N 15/85; C07H 21/04

IPC 8 full level

C12N 15/09 (2006.01); C07H 21/04 (2006.01); C07K 14/435 (2006.01); C12N 7/00 (2006.01); C12N 15/86 (2006.01); C12N 15/866 (2006.01)

CPC (source: EP)

C07K 14/43563 (2013.01); C12N 15/86 (2013.01); C07K 2319/02 (2013.01); C12N 2710/14143 (2013.01); C12N 2830/00 (2013.01); C12N 2830/002 (2013.01); C12N 2830/60 (2013.01)

Citation (search report)

  • [A] US 5155037 A 19921013 - SUMMERS MAX D [US]
  • [PA] WO 9404699 A1 19940303 - US HEALTH [US]
  • [A] EP 0228036 A2 19870708 - MICROGENESYS INC [US]
  • [XD] KITTS, P.A. ET AL.: "Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors", NUCLEIC ACIDS RESEARCH, vol. 18, no. 19, 1990, pages 5667 - 5672, XP002100321
  • [XD] KITTS, P.A. AND POSSEE, R.D.: "A method for producing recombinant baculovirus expression vectors at high frequency", BIOTECHNIQUES, vol. 14, no. 5, 1993, pages 810 - 817, XP002100322
  • [X] WEBB, N.R. AND SUMMERS, M.D.: "Expression of proteins using recombinant baculovirus vectors", TECHNIQUE, vol. 2, no. 4, 1990, pages 173 - 188, XP002100323
  • [AD] PEAKMAN, T.C. ET AL.: "Highly efficient generation of recombinant baculoviruses by enzymatically mediated site-specific in vitro recombination", NUCLEIC ACIDS RESEARCH, vol. 20, no. 3, 11 February 1992 (1992-02-11), pages 495-500, XP000670349
  • [A] MCCUTCHEN, B.F. ET AL.: "Development of a recombinant baculovirus expressing an insect-selective neurotoxin: potential for pest control", BIOTECHNOLOGY, vol. 9, no. 9, 1991, pages 848 - 852, XP002100324
  • See references of WO 9428114A1

Designated contracting state (EPC)

AT BE CH DE DK ES FR GB GR IE IT LI LU NL PT SE

DOCDB simple family (publication)

WO 9428114 A1 19941208; AU 684799 B2 19980108; AU 7048794 A 19941220; CA 2141206 A1 19941208; EP 0658196 A1 19950621; EP 0658196 A4 19990707; JP H07509617 A 19951026; TW 502065 B 20020911

DOCDB simple family (application)

US 9406079 W 19940527; AU 7048794 A 19940527; CA 2141206 A 19940527; EP 94919295 A 19940527; JP 50102395 A 19940527; TW 82109578 A 19931116