Global Patent Index - EP 0666987 A4

EP 0666987 A4 19970827 - MEASUREMENT OF TOTAL MOLECULE IN A SAMPLE AND METHODS BASED THEREON.

Title (en)

MEASUREMENT OF TOTAL MOLECULE IN A SAMPLE AND METHODS BASED THEREON.

Title (de)

MESSUNG DER GESAMTZAHL VON MOLEKÜLEN IN EINER PROBE UND DARAUF BERUHENDE VERFAHREN.

Title (fr)

EVALUATION DE LA QUANTITE TOTALE D'UNE MOLECULE DANS UN ECHANTILLON ET PROCEDES BASES SUR CELLE-CI.

Publication

EP 0666987 A4 19970827 (EN)

Application

EP 93925154 A 19931029

Priority

  • US 9310550 W 19931029
  • US 96879392 A 19921030

Abstract (en)

[origin: WO9410571A1] The measurement of the total amount of a target molecule is used in the detection, diagnosis, determining the severity of and staging of a variety of diseases or disorders and the monitoring of therapy. The measurements of the total amount of a target molecule is accomplished by treating a sample containing cells, such as a body fluid, with a non-ionic detergent in a manner that makes available for binding to one or more binding partners the total amount of a target molecule present in the cell membrane, the cytoplasm of the cell and/or in the soluble compartments of the body fluid. Once the total target molecule is available, it is measured using any of a number of assay techniques, preferably by sandwich enzyme immunoassay. The methods of the present application may be used to detect and measure molecules associated with microbial pathogens, in particular intracellular pathogens such as Chlamydia, as well as erythrocyte blood group antigens, tumor antigens, cytokines and cell adhesion molecules, for diagnosis, enumeration of cells expressing such molecules, and other methods.

IPC 1-7

G01N 33/53; G01N 33/536; G01N 33/541; G01N 33/543; G01N 33/563; G01N 33/569; G01N 33/577; C12Q 1/00; C12Q 1/04; C11D 1/00; C11D 1/66

IPC 8 full level

G01N 33/53 (2006.01); C12Q 1/68 (2006.01); G01N 33/531 (2006.01); G01N 33/543 (2006.01); G01N 33/569 (2006.01); G01N 33/571 (2006.01); G01N 33/574 (2006.01); G01N 33/68 (2006.01)

CPC (source: EP)

C12Q 1/6806 (2013.01); C12Q 1/6886 (2013.01); G01N 33/569 (2013.01); G01N 33/56911 (2013.01); G01N 33/56972 (2013.01); G01N 33/56983 (2013.01); G01N 33/571 (2013.01); G01N 33/6863 (2013.01); C12Q 2600/106 (2013.01)

Citation (search report)

Designated contracting state (EPC)

AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 9410571 A1 19940511; AU 5457194 A 19940524; AU 686577 B2 19980212; CA 2148219 A1 19940511; CA 2148219 C 19981229; EP 0666987 A1 19950816; EP 0666987 A4 19970827; JP H07509783 A 19951026

DOCDB simple family (application)

US 9310550 W 19931029; AU 5457194 A 19931029; CA 2148219 A 19931029; EP 93925154 A 19931029; JP 51140094 A 19931029