EP 0731837 A1 19960918 - BACTERIA USED TO PRODUCE STABLE FUSION PROTEINS AND METHOD FOR THEIR IDENTIFICATION
Title (en)
BACTERIA USED TO PRODUCE STABLE FUSION PROTEINS AND METHOD FOR THEIR IDENTIFICATION
Title (de)
BAKTERIEN ZUR HERSTELLUNG STABILER FUSIONSPROTEINE UND VERFAHREN ZU DEREN NACHWEIS
Title (fr)
BACTERIES UTILISEES POUR PREPARER DES PROTEINES DE FUSION STABLES ET PROCEDE PERMETTANT DE LES IDENTIFIER
Publication
Application
Priority
- DE 4344350 A 19931223
- EP 9404286 W 19941222
Abstract (en)
[origin: WO9517509A1] The invention pertains to bacteria used to produce stable fusion proteins from a carrier protein and a passenger protein, with the bacteria having the genetic marker fpt. Having this genetic marker allows a better production of protein fusions, which have a destabilizing effect on bacteria. In particular, the invention pertains to bacteria used to produce fusion proteins where the fusion protein is presented stably on the surface and the bacteria have the genetic markers ompT<-> and dsbA<-> in addition to the marker fpt. The invention also pertains to methods for identifying bacteria which on their surface present heterologous proteins with an affinity to a bonding partner and methods for constructing vectors coding for these proteins. Finally, the invention pertains also to bacteria which present stably at least one fusion protein on their surface and have the genetic markers fpt, ompT<-> and dsbA<->, and to their use, for example, for diagnostic purposes.
[origin: WO9517509A1] The invention pertains to bacteria used to produce stable fusion proteins from a carrier protein and a passenger protein, with the bacteria having the genetic marker fpt. Having this genetic marker allows a better production of protein fusions, which have a destabilizing effect on bacteria. In particular, the invention pertains to bacteria used to produce fusion proteins where the fusion protein is presented stably on the surface and the bacteria have the genetic markers ompT<-> and dsbA<-> in addition to the marker fpt. The invention also pertains to methods for identifying bacteria which on their surface present heterologous proteins with an affinity to a bonding partner and methods for constructing vectors coding for these proteins. Finally, the invention pertains also to bacteria which present stably at least one fusion protein on their surface and have the genetic markers fpt, ompT<-> and dsbA<->, and to their use, for example, for diagnostic purposes.
IPC 1-7
C12N 15/12; C12N 15/62; C12N 15/65; C12N 15/10; C12N 15/70; C12N 1/21
IPC 8 full level
C07K 16/00 (2006.01); C12N 1/21 (2006.01); C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12N 15/12 (2006.01); C12N 15/62 (2006.01); C12N 15/65 (2006.01); C12N 15/70 (2006.01); C12P 21/02 (2006.01); C12Q 1/02 (2006.01); C40B 40/02 (2006.01); C12R 1/19 (2006.01)
CPC (source: EP US)
C07K 16/00 (2013.01 - EP US); C12N 15/1037 (2013.01 - EP US); C12N 15/62 (2013.01 - EP US); C12N 15/65 (2013.01 - EP US); C12N 15/70 (2013.01 - EP US); C40B 40/02 (2013.01 - EP US); C07K 2319/00 (2013.01 - EP US); C07K 2319/02 (2013.01 - EP US)
Citation (search report)
See references of WO 9517509A1
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
DE 4344350 A1 19950629; DE 4344350 C2 19950921; EP 0731837 A1 19960918; JP H11514201 A 19991207; US 6040141 A 20000321; WO 9517509 A1 19950629
DOCDB simple family (application)
DE 4344350 A 19931223; EP 9404286 W 19941222; EP 95905579 A 19941222; JP 51720794 A 19941222; US 66635496 A 19960923