EP 0938299 A1 19990901 - METHODS FOR THE PRODUCTION OF PROTEIN PARTICLES USEFUL FOR DELIVERY OF PHARMACOLOGICAL AGENTS
Title (en)
METHODS FOR THE PRODUCTION OF PROTEIN PARTICLES USEFUL FOR DELIVERY OF PHARMACOLOGICAL AGENTS
Title (de)
VERFAHREN ZUR HERSTELLUNG VON PROTEINPARTIKELN ZUR VERABREICHUNG PHARMAKOLOGISCHER STOFFE
Title (fr)
PROCEDES DE PRODUCTION DE PARTICULES DE PROTEINES UTILES DANS LA DIFFUSION D'AGENTS PHARMACOLOGIQUES
Publication
Application
Priority
- US 9714661 W 19970819
- US 2396896 P 19960819
Abstract (en)
[origin: WO9807410A1] A method has been developed for the formation of submicron particles (nanoparticles) by heat-denaturation of proteins (such as human serum albumin) in the presence of multivalent ions (such as calcium). Also provided are novel products produced by the invention method. An appropriate concentration of multivalent ions, within a relatively narrow range of concentrations, induces the precipitation of protein in the form of colloidal particles, at a temperature which is well below the heat denaturation temperature of the protein (as low as 60 DEG C for serum albumin). Temperatures at which invention method operates are sufficiently low to permit incorporation of other molecules (e.g., by co-precipitation), into submicron particles according to the invention, including compounds which cannot withstand high temperatures. Invention methods facilitate the production of protein nanoparticles and microparticles containing various molecules (such as nucleic acids, oligonucleotides, polynucleotides, DNA, RNA, polysaccharides, ribozymes, pharmacologically active compounds, and the like) useful for therapeutic, diagnostic and other purposes. The addition of multivalent cations serves both to induce precipitation, and to allow linking of negatively charged molecules, such as DNA, to the negatively charged protein.
IPC 1-7
IPC 8 full level
A61K 9/16 (2006.01); A61K 9/51 (2006.01); A61K 49/22 (2006.01)
CPC (source: EP)
A61K 9/1658 (2013.01); A61K 9/5169 (2013.01); A61K 9/5192 (2013.01); A61K 49/223 (2013.01)
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9807410 A1 19980226; AU 3916997 A 19980306; CA 2263765 A1 19980226; CA 2263765 C 20100330; EP 0938299 A1 19990901; EP 0938299 A4 20010117
DOCDB simple family (application)
US 9714661 W 19970819; AU 3916997 A 19970819; CA 2263765 A 19970819; EP 97936517 A 19970819