EP 0938586 A4 20020130 - KINASE ACTIVITY MEASUREMENT USING FLUORESCENCE POLARIZATION
Title (en)
KINASE ACTIVITY MEASUREMENT USING FLUORESCENCE POLARIZATION
Title (de)
MESSUNG DER KINASEAKTIVITÄT MITTELS FLUORESZENTPOLARISATION
Title (fr)
MESURE DE L'ACTIVITE DE LA KINASE PAR POLARISATION DE FLUORESCENCE
Publication
Application
Priority
- US 9719570 W 19971028
- US 2983196 P 19961028
Abstract (en)
[origin: WO9818956A1] A method for quantitating enzyme activity of phosphorylation and dephosphorylation of a peptide or protein substrate, comprising measuring the fluorescence polarization of a fluorescence-emitting reporter molecule in solution with the phosphate. Then, adding an enzyme, either a kinase or a phosphatase, and incubating the solution. Finally, measuring the fluorescence polarization of the solution after the enzyme has had an opportunity to react with the peptide or protein substrate.
IPC 1-7
IPC 8 full level
G01N 33/542 (2006.01); G01N 33/573 (2006.01); G01N 33/68 (2006.01)
CPC (source: EP)
G01N 33/542 (2013.01); G01N 33/573 (2013.01); G01N 33/68 (2013.01); G01N 33/6842 (2013.01)
Citation (search report)
- [PX] WO 9739326 A2 19971023 - ARIAD PHARMA INC [US], et al
- [OPX] SEETHALA R, MENZEL R, SQUIBB BRISTOL-MYERS: "A Fluorescence Polarization Tyrosine Kinase Assay for High Thr oughput Screening", ANNUAL CONFERENCE OF THE SOCIETY FOR BIOMOLECULAR SCREENING, XX, XX, no. 3, 22 September 1997 (1997-09-22) - 25 September 1997 (1997-09-25), XX, pages 6 - 59, XP002968015 & US 6203994 B1 20010320 - EPPS DENNIS E [US], et al
- [Y] DANDLIKER, WALTER B. ET AL: "Equilibrium and kinetic inhibition assays based upon fluorescence polarization", METHODS ENZYMOL. (1981), 74(IMMUNOCHEM. TECH., PT. C), 3-28, 1981, XP001029241
- [Y] CHECOVICH W J ET AL: "Fluorescence polaritation - a new tool for cell and molecular biology", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 375, 18 May 1995 (1995-05-18), pages 254 - 256, XP002100426, ISSN: 0028-0836
- [Y] ZEMLAN F P ET AL: "MONOCLONAL ANTIBODY PHF-9 RECOGNIZES PHOSPHORYLATED SERINE 404 OF TAU PROTEIN AND LABELS PAIRED HELICAL FILAMENTS", JOURNAL OF NEUROSCIENCE RESEARCH, WILEY-LISS, US, vol. 46, no. 1, 1 October 1996 (1996-10-01), pages 90 - 97, XP000646724, ISSN: 0360-4012
- [Y] WRIGHT D E ET AL: "FLUOROMETRIC ASSAY FOR CYCLIC AMP DEPENDENT PROTEIN KINASE EC-2.7.1.37 AND PHOSPHO PROTEIN PHOSPHATASE EC-3.1.3.16 ACTIVITIES", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 78, no. 10, 1981, 1981, pages 6048 - 6050, XP001029505, ISSN: 0027-8424
- [A] Z-Y ZHANG ET AL: "A Continuous Spectrophotometric and Fluorimetric Assay for Protein Tyrosine Phosphatase Using Phosphotyrosine-Containing Peptides", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 211, 1993, pages 7 - 15, XP002100427, ISSN: 0003-2697
- See references of WO 9818956A1
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB IE IT LI NL SE
DOCDB simple family (publication)
WO 9818956 A1 19980507; EP 0938586 A1 19990901; EP 0938586 A4 20020130
DOCDB simple family (application)
US 9719570 W 19971028; EP 97952171 A 19971028