EP 1064536 A2 20010103 - METHOD FOR ENHANCING THE CONTRAST FOR A TRANSMISSION ELECTRON MICROSCOPE
Title (en)
METHOD FOR ENHANCING THE CONTRAST FOR A TRANSMISSION ELECTRON MICROSCOPE
Title (de)
VERFAHREN ZUR KONTRASTVERSTÄRKUNG FÜR EIN TRANSMISSIONSELEKTRONENMIKROSKOP
Title (fr)
MICROSCOPE ELECTRONIQUE A TRANSMISSION
Publication
Application
Priority
- DE 9900727 W 19990312
- DE 19811395 A 19980316
Abstract (en)
[origin: US6563112B1] The intention is to provide a process for the contrast enhancement of a specific particle in an image of a specimen, taken by a transmission electron microscope, in which a calculated contrast-rich image is created by way of the background intensities calculated from the intensities of a first image being drawn off pixel by pixel, whereby the background intensities are calculated as a function of the intensities of a second image. In this situation, it is intended that this process should feature a higher range of application than processes known hitherto, and, in particular, are well-suited for contrast enhancement for gold particles in immuno-gold marking. It is proposed, for this purpose, that the first image be taken under conditions in which the particle features the highest possible contrast, and that the second image is taken in a selected energy window which is selected in such a way that the contrast difference between the two images for the particle differs by the corresponding contrast difference for at least a second specimen constituent.
IPC 1-7
IPC 8 full level
G01N 23/04 (2006.01); G01N 23/08 (2006.01); G01Q 30/02 (2010.01); G01Q 30/04 (2010.01); H01J 37/22 (2006.01); H01J 37/28 (2006.01)
CPC (source: EP US)
G01N 23/06 (2013.01 - EP US); H01J 37/28 (2013.01 - EP US)
Citation (search report)
See references of WO 9947910A2
Designated contracting state (EPC)
AT BE CH DE ES FR GB LI LU NL SE
DOCDB simple family (publication)
US 6563112 B1 20030513; AU 3809599 A 19991011; DE 19811395 A1 19990923; EP 1064536 A2 20010103; JP 2002506992 A 20020305; WO 9947910 A2 19990923; WO 9947910 A3 20000113
DOCDB simple family (application)
US 62393300 A 20001027; AU 3809599 A 19990312; DE 19811395 A 19980316; DE 9900727 W 19990312; EP 99920547 A 19990312; JP 2000537054 A 19990312