Global Patent Index - EP 1064536 A2

EP 1064536 A2 2001-01-03 - METHOD FOR ENHANCING THE CONTRAST FOR A TRANSMISSION ELECTRON MICROSCOPE

Title (en)

METHOD FOR ENHANCING THE CONTRAST FOR A TRANSMISSION ELECTRON MICROSCOPE

Title (de)

VERFAHREN ZUR KONTRASTVERSTÄRKUNG FÜR EIN TRANSMISSIONSELEKTRONENMIKROSKOP

Title (fr)

MICROSCOPE ELECTRONIQUE A TRANSMISSION

Publication

EP 1064536 A2 (DE)

Application

EP 99920547 A

Priority

  • DE 9900727 W
  • DE 19811395 A

Abstract (en)

[origin: US6563112B1] The intention is to provide a process for the contrast enhancement of a specific particle in an image of a specimen, taken by a transmission electron microscope, in which a calculated contrast-rich image is created by way of the background intensities calculated from the intensities of a first image being drawn off pixel by pixel, whereby the background intensities are calculated as a function of the intensities of a second image. In this situation, it is intended that this process should feature a higher range of application than processes known hitherto, and, in particular, are well-suited for contrast enhancement for gold particles in immuno-gold marking. It is proposed, for this purpose, that the first image be taken under conditions in which the particle features the highest possible contrast, and that the second image is taken in a selected energy window which is selected in such a way that the contrast difference between the two images for the particle differs by the corresponding contrast difference for at least a second specimen constituent.

IPC 1-7 (main, further and additional classification)

G01N 23/08; H01J 37/28

IPC 8 full level (invention and additional information)

G01N 23/04 (2006.01); G01N 23/08 (2006.01); G01Q 30/02 (2010.01); G01Q 30/04 (2010.01); H01J 37/22 (2006.01); H01J 37/28 (2006.01)

CPC (invention and additional information)

G01N 23/08 (2013.01); H01J 37/28 (2013.01)

Citation (search report)

See references of WO 9947910A3

Designated contracting state (EPC)

AT BE CH DE ES FR GB LI LU NL SE

EPO simple patent family

US 6563112 B1 20030513; AU 3809599 A 19991011; DE 19811395 A1 19990923; EP 1064536 A2 20010103; JP 2002506992 A 20020305; WO 9947910 A2 19990923; WO 9947910 A3 20000113

INPADOC legal status


2007-05-02 [18D] DEEMED TO BE WITHDRAWN

- Effective date: 20061003

2001-01-03 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20000906

2001-01-03 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE CH DE ES FR GB LI LU NL SE