EP 1066371 A1 20010110 - EUKARYOTIC CELLS STABLY EXPRESSING GENES FROM MULTIPLE TRANSFECTED EPISOMES
Title (en)
EUKARYOTIC CELLS STABLY EXPRESSING GENES FROM MULTIPLE TRANSFECTED EPISOMES
Title (de)
EUKARYOTISCHE ZELLEN, WELCHE AUSGEHEND VON MULTIPLEN TRANSFIZIERTEN EPISOMEN, GENE STABIL EXRIMIEREN
Title (fr)
CELLULES EUCARYOTES EXPRIMANT DES GENES DE MANIERE STABLE A PARTIR DE MULTIPLES EPISOMES TRANSFECTES
Publication
Application
Priority
- US 9903307 W 19990212
- US 4096198 A 19980318
- US 13011498 A 19980806
Abstract (en)
[origin: WO9947647A1] A method is described for producing recombinant eukaryotic cell lines expressing multiple proteins or RNAs of interest. Eukaryotic host cells are transfected with (a) a first episome which contains a sequence that promotes autonomous replication of the episome in the cells and a first gene encoding a protein of interest; and (b) a second episome containing a sequence that promotes autonomous replication of the episome in the cells and a second gene encoding a protein of interest. Transfected cells are obtained expressing one or more proteins that promote nuclear retention of the episomes. The cells are grown under conditions wherein the episomes express the first and second genes.
IPC 1-7
IPC 8 full level
C12N 15/10 (2006.01)
CPC (source: EP)
C12N 15/10 (2013.01)
Citation (search report)
See references of WO 9947647A1
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9947647 A1 19990923; AU 2767999 A 19991011; CA 2324518 A1 19990923; EP 1066371 A1 20010110; IL 138523 A0 20011031
DOCDB simple family (application)
US 9903307 W 19990212; AU 2767999 A 19990212; CA 2324518 A 19990212; EP 99908186 A 19990212; IL 13852399 A 19990212