EP 1218501 A1 20020703 - CREATION OF VARIABLE LENGTH AND SEQUENCE LINKER REGIONS FOR DUAL-DOMAIN OR MULTI-DOMAIN MOLECULES
Title (en)
CREATION OF VARIABLE LENGTH AND SEQUENCE LINKER REGIONS FOR DUAL-DOMAIN OR MULTI-DOMAIN MOLECULES
Title (de)
VORBEREITUNG VON LINKER-REGIONEN MIT VARIABLER LÄNGE ZUR HERSTELLUNG VON BI- ODER MULTIDOMÄNPROTEINE
Title (fr)
CREATION DE REGIONS DE LIAISON A SEQUENCE ET LONGUEUR VARIABLES, POUR MOLECULES A DOMAINE DOUBLE OU MULTIPLE
Publication
Application
Priority
- US 0025965 W 20000922
- US 15597899 P 19990924
Abstract (en)
[origin: WO0123543A1] Disclosed are methods and compositions for creating a DNA, RNA or protein molecule with two or more nucleic acid or polypeptide domains, respectively, joined by a linker region. These methods are used to generate random linker libraries of nucleic acids that encode dual-domain or multi-domain polypeptides. The linker regions are characterized by both length and sequence variability.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C07K 14/705 (2006.01); C07K 16/00 (2006.01); C07K 19/00 (2006.01); C12N 15/10 (2006.01); C12N 15/62 (2006.01); C12N 15/83 (2006.01); C12P 21/02 (2006.01)
CPC (source: EP KR)
C07K 14/70503 (2013.01 - EP); C12N 15/10 (2013.01 - KR); C12N 15/1093 (2013.01 - EP); C12N 15/62 (2013.01 - EP)
Citation (search report)
See references of WO 0123543A1
Citation (examination)
- D.J. TURNER, M.A. RITTER & A.J.T. GEORGE: "Importance of the linker in expression of single-chain Fv antibody fragments: optimisation of peptide sequence using phage display technology", J. IMMUNOL. METHODS, vol. 205, 1997, pages 43 - 54
- Y. TANG, N. JIANG, C. PARAKH & D. HILVERT: "Selection of linkers for a catalytic single-chain antibody using phage display technology.", J. BIOL. CHEM., vol. 271, no. 26, 1996, pages 15682 - 15686
- A. SIMONCSITS, M.L. TJÖRNHAMMAR, S. WANG & S. PONGOR: "Isolation of altered specificity mutants of the single-chain 434 repressor that recognize asymetric DNA sequences containing the TTA and TTAC subsites.", NUCL. ACIDS RES., vol. 27, no. 17, September 1999 (1999-09-01), pages 3474 - 3480
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0123543 A1 20010405; AU 7601700 A 20010430; AU 782856 B2 20050901; CA 2385609 A1 20010405; EP 1218501 A1 20020703; JP 2003510073 A 20030318; KR 20020059413 A 20020712; RU 2002110820 A 20040127; ZA 200202066 B 20030528
DOCDB simple family (application)
US 0025965 W 20000922; AU 7601700 A 20000922; CA 2385609 A 20000922; EP 00965277 A 20000922; JP 2001526926 A 20000922; KR 20027003842 A 20020323; RU 2002110820 A 20000922; ZA 200202066 A 20020313